Carg01161 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg01161 vs. NCBI nr
Match: KAG7027843.1 (hypothetical protein SDJN02_09020, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 982.2 bits (2538), Expect = 1.6e-282
Identity = 488/488 (100.00%), Postives = 488/488 (100.00%), Query Frame = 0

Query: 1   MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 60
           MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL
Sbjct: 1   MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 60

Query: 61  LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 120
           LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL
Sbjct: 61  LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 120

Query: 121 VSEGINQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD 180
           VSEGINQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD
Sbjct: 121 VSEGINQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD 180

Query: 181 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 240
           GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN
Sbjct: 181 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 240

Query: 241 RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGY 300
           RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGY
Sbjct: 241 RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGY 300

Query: 301 QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN 360
           QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN
Sbjct: 301 QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN 360

Query: 361 QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNG 420
           QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNG
Sbjct: 361 QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNG 420

Query: 421 DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK 480
           DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK
Sbjct: 421 DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK 480

Query: 481 FTSGFGAN 489
           FTSGFGAN
Sbjct: 481 FTSGFGAN 488

BLAST of Carg01161 vs. NCBI nr
Match: KAG6596294.1 (Histone acetyltransferase HAC1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 982.2 bits (2538), Expect = 1.6e-282
Identity = 488/488 (100.00%), Postives = 488/488 (100.00%), Query Frame = 0

Query: 1    MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 60
            MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL
Sbjct: 3211 MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 3270

Query: 61   LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 120
            LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL
Sbjct: 3271 LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 3330

Query: 121  VSEGINQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD 180
            VSEGINQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD
Sbjct: 3331 VSEGINQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD 3390

Query: 181  GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 240
            GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN
Sbjct: 3391 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 3450

Query: 241  RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGY 300
            RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGY
Sbjct: 3451 RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGY 3510

Query: 301  QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN 360
            QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN
Sbjct: 3511 QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN 3570

Query: 361  QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNG 420
            QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNG
Sbjct: 3571 QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNG 3630

Query: 421  DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK 480
            DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK
Sbjct: 3631 DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK 3690

Query: 481  FTSGFGAN 489
            FTSGFGAN
Sbjct: 3691 FTSGFGAN 3698

BLAST of Carg01161 vs. NCBI nr
Match: XP_023539547.1 (pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 978.4 bits (2528), Expect = 2.3e-281
Identity = 486/488 (99.59%), Postives = 487/488 (99.80%), Query Frame = 0

Query: 1   MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 60
           MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL
Sbjct: 136 MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 195

Query: 61  LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 120
           LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL
Sbjct: 196 LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 255

Query: 121 VSEGINQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD 180
           VSEGINQLTSQVYNLLSMVKSISNQPAGKGES+FPAWLKTEEQSLLQQNDFAADATVAAD
Sbjct: 256 VSEGINQLTSQVYNLLSMVKSISNQPAGKGESKFPAWLKTEEQSLLQQNDFAADATVAAD 315

Query: 181 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 240
           GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN
Sbjct: 316 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 375

Query: 241 RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGY 300
           RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGY
Sbjct: 376 RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGY 435

Query: 301 QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN 360
           QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN
Sbjct: 436 QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN 495

Query: 361 QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNG 420
           QPTGFSIQFCNISADSDLQP VNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNG
Sbjct: 496 QPTGFSIQFCNISADSDLQPLVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNG 555

Query: 421 DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK 480
           DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK
Sbjct: 556 DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK 615

Query: 481 FTSGFGAN 489
           FTSGFGAN
Sbjct: 616 FTSGFGAN 623

BLAST of Carg01161 vs. NCBI nr
Match: XP_022949597.1 (pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita moschata])

HSP 1 Score: 978.4 bits (2528), Expect = 2.3e-281
Identity = 485/488 (99.39%), Postives = 486/488 (99.59%), Query Frame = 0

Query: 1   MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 60
           MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL
Sbjct: 22  MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 81

Query: 61  LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 120
           LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL
Sbjct: 82  LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 141

Query: 121 VSEGINQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD 180
           VSEGINQLTSQVYNLLSMVKSISNQP GKGESEFPAWLKTEEQSLLQQNDFAADATVAAD
Sbjct: 142 VSEGINQLTSQVYNLLSMVKSISNQPVGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD 201

Query: 181 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 240
           GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN
Sbjct: 202 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 261

Query: 241 RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGY 300
           RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRM GY
Sbjct: 262 RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMRGY 321

Query: 301 QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN 360
           QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN
Sbjct: 322 QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN 381

Query: 361 QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNG 420
           QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSY+SNAIRPEGWLEWNG
Sbjct: 382 QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYVSNAIRPEGWLEWNG 441

Query: 421 DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK 480
           DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK
Sbjct: 442 DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK 501

Query: 481 FTSGFGAN 489
           FTSGFGAN
Sbjct: 502 FTSGFGAN 509

BLAST of Carg01161 vs. NCBI nr
Match: XP_022971304.1 (pectinesterase/pectinesterase inhibitor PPE8B-like [Cucurbita maxima])

HSP 1 Score: 969.1 bits (2504), Expect = 1.4e-278
Identity = 482/489 (98.57%), Postives = 486/489 (99.39%), Query Frame = 0

Query: 1   MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 60
           +APAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL
Sbjct: 110 LAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 169

Query: 61  LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 120
           LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL
Sbjct: 170 LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 229

Query: 121 VSEGINQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD 180
           VSEGINQLTSQVYNLLSMVKSISNQPAGKGES FPAWLK+EEQSLLQQNDFAADATVAAD
Sbjct: 230 VSEGINQLTSQVYNLLSMVKSISNQPAGKGESGFPAWLKSEEQSLLQQNDFAADATVAAD 289

Query: 181 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 240
           GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN
Sbjct: 290 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 349

Query: 241 RSFIDGWTTFRSATFAVSG-RGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSG 300
           RSFIDGWTTFRSATFAVSG RGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSG
Sbjct: 350 RSFIDGWTTFRSATFAVSGRRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSG 409

Query: 301 YQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDP 360
           YQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDP
Sbjct: 410 YQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDP 469

Query: 361 NQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWN 420
           NQPTGFSIQFCNISADSDLQPSVN TATYLGRPWKEYSRTIIMQSY+SNAIRPEGWLEWN
Sbjct: 470 NQPTGFSIQFCNISADSDLQPSVNTTATYLGRPWKEYSRTIIMQSYMSNAIRPEGWLEWN 529

Query: 421 GDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGV 480
           GDFALNTLFYAEFMNYGPGAGLAKR+NWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGV
Sbjct: 530 GDFALNTLFYAEFMNYGPGAGLAKRINWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGV 589

Query: 481 KFTSGFGAN 489
           KFTSGFGAN
Sbjct: 590 KFTSGFGAN 598

BLAST of Carg01161 vs. ExPASy Swiss-Prot
Match: Q43062 (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 2.0e-208
Identity = 352/479 (73.49%), Postives = 408/479 (85.18%), Query Frame = 0

Query: 9   QTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQL 68
           QT+CLKV ++EF+ S+  TI  V+QV  I S F    GD RL+ AISDCLDLLD S+D+L
Sbjct: 45  QTQCLKVSATEFAGSLKDTIDAVQQVASILSQFANAFGDFRLANAISDCLDLLDFSADEL 104

Query: 69  SWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQL 128
           +WSLSA+QN K KNNSTG LSSDL+TWLSAA VN +TC  GFEGTNSIV+GL+S G+ Q+
Sbjct: 105 NWSLSASQNQKGKNNSTGKLSSDLRTWLSAALVNQDTCSNGFEGTNSIVQGLISAGLGQV 164

Query: 129 TSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAADGTGDFTTV 188
           TS V  LL+ V   SNQ    G  + P+W+KT+++ LLQ +  + DA VA DGTG+FT V
Sbjct: 165 TSLVQELLTQVHPNSNQQGPNG--QIPSWVKTKDRKLLQADGVSVDAIVAQDGTGNFTNV 224

Query: 189 MEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWT 248
            +AVLAAPD SMRRYVIY+K+G Y ENVEIKKKKWN+MMIGDG+DAT+ISGNRSF+DGWT
Sbjct: 225 TDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMDATIISGNRSFVDGWT 284

Query: 249 TFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLYTHT 308
           TFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVF+RC + GYQDTLYTHT
Sbjct: 285 TFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRGYQDTLYTHT 344

Query: 309 MRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQ 368
           MRQFYR+C ISGTVDF+FGDATVVFQNC ILAKKGLPNQKN+ITAQGRKDPN+PTG SIQ
Sbjct: 345 MRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDPNEPTGISIQ 404

Query: 369 FCNISADSDLQ-PSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFALNTL 428
           FCNI+ADSDL+  SVN+T TYLGRPWK YSRT+IMQS++SN IRPEGWLEWNGDFALN+L
Sbjct: 405 FCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEWNGDFALNSL 464

Query: 429 FYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSGFG 487
           FY E+MNYGPGAGL  RV WPGY   N++++A N+TV+QFIEGNLWLPSTGVK+T+ FG
Sbjct: 465 FYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTGVKYTAEFG 521

BLAST of Carg01161 vs. ExPASy Swiss-Prot
Match: Q9SMY7 (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3702 GN=PME44 PE=2 SV=2)

HSP 1 Score: 667.5 bits (1721), Expect = 1.1e-190
Identity = 326/499 (65.33%), Postives = 390/499 (78.16%), Query Frame = 0

Query: 9   QTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQL 68
           Q + LKVP+SEF +S+ + + V+RQV  I S F  F GD RL  A+SDCLDLLD SS++L
Sbjct: 24  QYDYLKVPASEFVSSINTIVVVIRQVSSILSQFADFSGDRRLQNAVSDCLDLLDFSSEEL 83

Query: 69  SWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQL 128
           +WS SA++NPK K N TGD+ SD +TWLSAA  N  TCMEGF+GT+ +VK LV+  ++QL
Sbjct: 84  TWSASASENPKGKGNGTGDVGSDTRTWLSAALSNQATCMEGFDGTSGLVKSLVAGSLDQL 143

Query: 129 TSQVYNLLSMV------KSISNQ-PAGKGES----------------EFPAWLKTEEQSL 188
            S +  LL +V      K++S   P  KG                  +FP W++ +++ L
Sbjct: 144 YSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLRDTDEDESLQFPDWVRPDDRKL 203

Query: 189 LQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNI 248
           L+ N    D +VA DGTG+FT +M+A+  APD S  R+VIY+KKG+Y ENVEIKKKKWNI
Sbjct: 204 LESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNI 263

Query: 249 MMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRS 308
           +M+GDGID TVISGNRSFIDGWTTFRSATFAVSGRGF+ARDITF+NTAGPEKHQAVALRS
Sbjct: 264 VMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRS 323

Query: 309 DSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLP 368
           DSDLSVFFRC M GYQDTLYTHTMRQFYREC I+GTVDF+FGD TVVFQNC ILAK+GLP
Sbjct: 324 DSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLP 383

Query: 369 NQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSY 428
           NQKNTITAQGRKD NQP+GFSIQF NISAD+DL P +N T TYLGRPWK YSRT+ +++ 
Sbjct: 384 NQKNTITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNN 443

Query: 429 ISNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVS 485
           +S+ +RPEGWLEWN DFAL+TLFY EFMNYGPG+GL+ RV WPGYH  N + +A NFTVS
Sbjct: 444 MSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVS 503

BLAST of Carg01161 vs. ExPASy Swiss-Prot
Match: Q9LXK7 (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3702 GN=PME32 PE=2 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 2.5e-190
Identity = 321/494 (64.98%), Postives = 401/494 (81.17%), Query Frame = 0

Query: 4   AADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDS 63
           + DL Q ECL+VP  EF+ +  + +  + + + I S F K  G SR+S AI DC+DLLDS
Sbjct: 31  STDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKAGKSRVSNAIVDCVDLLDS 90

Query: 64  SSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSE 123
           ++++LSW +SA+Q+P  K+NSTGD+ SDL+TW+SAA  N +TC++GFEGTN I+K +V+ 
Sbjct: 91  AAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFEGTNGIIKKIVAG 150

Query: 124 GINQLTSQVYNLLSMVKSISNQP------------AGKGESEFPAWLKTEEQSLLQ-QND 183
           G++++ + V NLL+MV S  ++P            A  G S+FP+W+K  ++ LLQ  N 
Sbjct: 151 GLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSWVKPGDRKLLQTDNI 210

Query: 184 FAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGD 243
             ADA VAADGTG+FTT+ +AVLAAPD S +RYVI+VK+GVY ENVEIKKKKWNIMM+GD
Sbjct: 211 TVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGD 270

Query: 244 GIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLS 303
           GIDATVI+GNRSFIDGWTTFRSATFAVSGRGFIARDITF+NTAGPEKHQAVA+RSD+DL 
Sbjct: 271 GIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLG 330

Query: 304 VFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNT 363
           VF+RC M GYQDTLY H+MRQF+REC I+GTVDF+FGDAT VFQ+C I AK+GLPNQKN+
Sbjct: 331 VFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNS 390

Query: 364 ITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAI 423
           ITAQGRKDPN+PTGF+IQF NI+AD+DL  ++N TATYLGRPWK YSRT+ MQ+Y+S+AI
Sbjct: 391 ITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAI 450

Query: 424 RPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEG 483
            P GWLEWNG+FAL+TL+Y E+MN GPGA L +RV WPGYH LN ++EA NFTVSQ I+G
Sbjct: 451 NPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQG 510

Query: 484 NLWLPSTGVKFTSG 485
           NLWLPSTG+ F +G
Sbjct: 511 NLWLPSTGITFIAG 524

BLAST of Carg01161 vs. ExPASy Swiss-Prot
Match: Q96575 (Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 9.0e-124
Identity = 227/489 (46.42%), Postives = 319/489 (65.24%), Query Frame = 0

Query: 5   ADLEQTECLKVPSSEFSNSVLSTIGVVRQV---MLIFSPFTKFLGDSRLSTAISDCLDLL 64
           +DL   E +   S   S  +   +  V Q+   + + S     + D R   A++DCL+LL
Sbjct: 68  SDLMSNEIVTTDSDGLSILMKFLVNYVHQMNNAIPVVSKMKNQINDIRQEGALTDCLELL 127

Query: 65  DSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEG-TNSIVKGL 124
           D S D +S S++A       +  T    ++ ++WLS    N  TC++  +  T +++ G 
Sbjct: 128 DQSVDLVSDSIAAI------DKRTHSEHANAQSWLSGVLTNHVTCLDELDSFTKAMING- 187

Query: 125 VSEGINQLTSQVYNLLSMVKSI---SNQPAGKGESEFPAWLKTEEQSLLQQN--DFAADA 184
               +++L S+    L+M+ S+   ++     G  + P+W+ + ++ L++ +  D  A+A
Sbjct: 188 --TNLDELISRAKVALAMLASVTTPNDDVLRPGLGKMPSWVSSRDRKLMESSGKDIGANA 247

Query: 185 TVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDAT 244
            VA DGTG + T+ EAV AAPDKS  RYVIYVK+G+Y ENVE+  +K  +M++GDG+ AT
Sbjct: 248 VVAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGIYKENVEVSSRKMKLMIVGDGMHAT 307

Query: 245 VISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRC 304
           +I+GN + +DG TTF SAT A  G+GFI +DI  +NTAGP KHQAVALR  +D SV  RC
Sbjct: 308 IITGNLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALRVGADKSVINRC 367

Query: 305 RMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQG 364
           R+  YQDTLY H+ RQFYR+ +++GT+DF+FG+A VVFQ C ++A+K    Q+N +TAQG
Sbjct: 368 RIDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCKLVARKPGKYQQNMVTAQG 427

Query: 365 RKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGW 424
           R DPNQ TG SIQFCNI A SDL+P +    TYLGRPWK+YSRT++M+SY+   I P GW
Sbjct: 428 RTDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKKYSRTVVMESYLGGLINPAGW 487

Query: 425 LEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLP 484
            EW+GDFAL TL+Y EFMN GPGAG +KRV WPGYH +   +EA  FTV++ I+G  WL 
Sbjct: 488 AEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHCITDPAEAMPFTVAKLIQGGSWLR 547

BLAST of Carg01161 vs. ExPASy Swiss-Prot
Match: P14280 (Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 438.3 bits (1126), Expect = 1.1e-121
Identity = 220/446 (49.33%), Postives = 301/446 (67.49%), Query Frame = 0

Query: 45  LGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPE 104
           + D R   A++DCL+LLD S D  S S++A        +      ++ ++WLS    N  
Sbjct: 107 INDIRQHGALTDCLELLDQSVDFASDSIAAIDKRSRSEH------ANAQSWLSGVLTNHV 166

Query: 105 TCMEGFEG-TNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQPAGKGES---EFPAWLKT 164
           TC++  +  T +++ G     + +L S+    L+M+ S++ Q      +   + P+W+ +
Sbjct: 167 TCLDELDSFTKAMING---TNLEELISRAKVALAMLASLTTQDEDVFMTVLGKMPSWVSS 226

Query: 165 EEQSLLQQN--DFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEI 224
            ++ L++ +  D  A+A VA DGTGD+ T+ EAV AAPDKS  RYVIYVK+G Y ENVE+
Sbjct: 227 MDRKLMESSGKDIIANAVVAQDGTGDYQTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEV 286

Query: 225 KKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKH 284
              K N+M++GDG+ AT I+G+ + +DG TTFRSAT A  G+GFI +DI  +NTAGP K 
Sbjct: 287 ASNKMNLMIVGDGMYATTITGSLNVVDGSTTFRSATLAAVGQGFILQDICIQNTAGPAKD 346

Query: 285 QAVALRSDSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTI 344
           QAVALR  +D+SV  RCR+  YQDTLY H+ RQFYR+ +++GTVDF+FG+A VVFQ C +
Sbjct: 347 QAVALRVGADMSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTVDFIFGNAAVVFQKCQL 406

Query: 345 LAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSR 404
           +A+K    Q+N +TAQGR DPNQ TG SIQFCNI A SDL+P +    TYLGRPWKEYSR
Sbjct: 407 VARKPGKYQQNMVTAQGRTDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKEYSR 466

Query: 405 TIIMQSYISNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSE 464
           T++M+SY+   I P GW EW+GDFAL TL+Y EFMN GPGAG +KRV WPGYH +   ++
Sbjct: 467 TVVMESYLGGLINPAGWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAK 526

Query: 465 ATNFTVSQFIEGNLWLPSTGVKFTSG 485
           A  FTV++ I+G  WL STGV +  G
Sbjct: 527 AMPFTVAKLIQGGSWLRSTGVAYVDG 543

BLAST of Carg01161 vs. ExPASy TrEMBL
Match: A0A6J1GCJ9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452924 PE=3 SV=1)

HSP 1 Score: 978.4 bits (2528), Expect = 1.1e-281
Identity = 485/488 (99.39%), Postives = 486/488 (99.59%), Query Frame = 0

Query: 1   MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 60
           MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL
Sbjct: 22  MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 81

Query: 61  LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 120
           LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL
Sbjct: 82  LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 141

Query: 121 VSEGINQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD 180
           VSEGINQLTSQVYNLLSMVKSISNQP GKGESEFPAWLKTEEQSLLQQNDFAADATVAAD
Sbjct: 142 VSEGINQLTSQVYNLLSMVKSISNQPVGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD 201

Query: 181 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 240
           GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN
Sbjct: 202 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 261

Query: 241 RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGY 300
           RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRM GY
Sbjct: 262 RSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMRGY 321

Query: 301 QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN 360
           QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN
Sbjct: 322 QDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPN 381

Query: 361 QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNG 420
           QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSY+SNAIRPEGWLEWNG
Sbjct: 382 QPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYVSNAIRPEGWLEWNG 441

Query: 421 DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK 480
           DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK
Sbjct: 442 DFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVK 501

Query: 481 FTSGFGAN 489
           FTSGFGAN
Sbjct: 502 FTSGFGAN 509

BLAST of Carg01161 vs. ExPASy TrEMBL
Match: A0A6J1I863 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470062 PE=3 SV=1)

HSP 1 Score: 969.1 bits (2504), Expect = 6.6e-279
Identity = 482/489 (98.57%), Postives = 486/489 (99.39%), Query Frame = 0

Query: 1   MAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 60
           +APAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL
Sbjct: 110 LAPAADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDL 169

Query: 61  LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 120
           LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL
Sbjct: 170 LDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGL 229

Query: 121 VSEGINQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAAD 180
           VSEGINQLTSQVYNLLSMVKSISNQPAGKGES FPAWLK+EEQSLLQQNDFAADATVAAD
Sbjct: 230 VSEGINQLTSQVYNLLSMVKSISNQPAGKGESGFPAWLKSEEQSLLQQNDFAADATVAAD 289

Query: 181 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 240
           GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN
Sbjct: 290 GTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGN 349

Query: 241 RSFIDGWTTFRSATFAVSG-RGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSG 300
           RSFIDGWTTFRSATFAVSG RGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSG
Sbjct: 350 RSFIDGWTTFRSATFAVSGRRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSG 409

Query: 301 YQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDP 360
           YQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDP
Sbjct: 410 YQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDP 469

Query: 361 NQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWN 420
           NQPTGFSIQFCNISADSDLQPSVN TATYLGRPWKEYSRTIIMQSY+SNAIRPEGWLEWN
Sbjct: 470 NQPTGFSIQFCNISADSDLQPSVNTTATYLGRPWKEYSRTIIMQSYMSNAIRPEGWLEWN 529

Query: 421 GDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGV 480
           GDFALNTLFYAEFMNYGPGAGLAKR+NWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGV
Sbjct: 530 GDFALNTLFYAEFMNYGPGAGLAKRINWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGV 589

Query: 481 KFTSGFGAN 489
           KFTSGFGAN
Sbjct: 590 KFTSGFGAN 598

BLAST of Carg01161 vs. ExPASy TrEMBL
Match: A0A5D3CHH9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00030 PE=3 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 7.7e-251
Identity = 431/484 (89.05%), Postives = 459/484 (94.83%), Query Frame = 0

Query: 5   ADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSS 64
           ADL QTECL VPSS+FSNS+LSTI VVRQVM IFSPF+K LGD RLSTAISDCLDLLDSS
Sbjct: 9   ADLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSS 68

Query: 65  SDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEG 124
           +DQLSWSLSA QNPKAK++STGDLSSDLKTWLSAA VNPETCM+GFEGTNSI+KGLVS G
Sbjct: 69  ADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGG 128

Query: 125 INQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAADGTGD 184
           INQLTSQ+Y+LLSMVKSI NQP     SEFP+WLK+E+++LLQ ND AADATVAADGTGD
Sbjct: 129 INQLTSQLYDLLSMVKSIPNQP-----SEFPSWLKSEDRNLLQLNDLAADATVAADGTGD 188

Query: 185 FTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFI 244
           FT VM+AVLAAPDKS RRYVIY+KKGVY ENVEIKKKKWN+MMIGDGIDAT+ISGNRSFI
Sbjct: 189 FTNVMDAVLAAPDKSTRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFI 248

Query: 245 DGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTL 304
           DGWTTFRSATFAVSGRGFIARDITFENTAG EKHQAVALRSDSDLSVFFRCR+ GYQDTL
Sbjct: 249 DGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTL 308

Query: 305 YTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTG 364
           YTHTMRQFYREC ISGTVDFLFGDATVVFQNC+ILAKKGLPNQKNTITAQGRKDPNQPTG
Sbjct: 309 YTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTG 368

Query: 365 FSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFAL 424
           FSIQFCNISADSDL+PSVN TATYLGRPWKEYSRTIIMQSYIS+AIRPEGWLEWNG+FAL
Sbjct: 369 FSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNGNFAL 428

Query: 425 NTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSG 484
           NTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTV+QFIEGN+WLPSTGVK+T+G
Sbjct: 429 NTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAG 487

Query: 485 FGAN 489
            GAN
Sbjct: 489 LGAN 487

BLAST of Carg01161 vs. ExPASy TrEMBL
Match: A0A1S3BKA0 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490630 PE=3 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 7.7e-251
Identity = 431/484 (89.05%), Postives = 459/484 (94.83%), Query Frame = 0

Query: 5   ADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSS 64
           ADL QTECL VPSS+FSNS+LSTI VVRQVM IFSPF+K LGD RLSTAISDCLDLLDSS
Sbjct: 98  ADLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSS 157

Query: 65  SDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEG 124
           +DQLSWSLSA QNPKAK++STGDLSSDLKTWLSAA VNPETCM+GFEGTNSI+KGLVS G
Sbjct: 158 ADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGG 217

Query: 125 INQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAADGTGD 184
           INQLTSQ+Y+LLSMVKSI NQP     SEFP+WLK+E+++LLQ ND AADATVAADGTGD
Sbjct: 218 INQLTSQLYDLLSMVKSIPNQP-----SEFPSWLKSEDRNLLQLNDLAADATVAADGTGD 277

Query: 185 FTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFI 244
           FT VM+AVLAAPDKS RRYVIY+KKGVY ENVEIKKKKWN+MMIGDGIDAT+ISGNRSFI
Sbjct: 278 FTNVMDAVLAAPDKSTRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFI 337

Query: 245 DGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTL 304
           DGWTTFRSATFAVSGRGFIARDITFENTAG EKHQAVALRSDSDLSVFFRCR+ GYQDTL
Sbjct: 338 DGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTL 397

Query: 305 YTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTG 364
           YTHTMRQFYREC ISGTVDFLFGDATVVFQNC+ILAKKGLPNQKNTITAQGRKDPNQPTG
Sbjct: 398 YTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTG 457

Query: 365 FSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFAL 424
           FSIQFCNISADSDL+PSVN TATYLGRPWKEYSRTIIMQSYIS+AIRPEGWLEWNG+FAL
Sbjct: 458 FSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNGNFAL 517

Query: 425 NTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSG 484
           NTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTV+QFIEGN+WLPSTGVK+T+G
Sbjct: 518 NTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAG 576

Query: 485 FGAN 489
            GAN
Sbjct: 578 LGAN 576

BLAST of Carg01161 vs. ExPASy TrEMBL
Match: A0A0A0L4S0 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G000060 PE=3 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 2.2e-250
Identity = 430/483 (89.03%), Postives = 457/483 (94.62%), Query Frame = 0

Query: 6   DLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSS 65
           DL QTECL VPSS+FSNS+LSTI VVRQVM IFSPF+K LGD RLSTAISDCLDLLDSS+
Sbjct: 99  DLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSA 158

Query: 66  DQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGI 125
           DQLSWSLSA QNPKAKN+STGDLSSDLKTWLSAA VNPETCM+GFEGTNSI+KGLVS G+
Sbjct: 159 DQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGV 218

Query: 126 NQLTSQVYNLLSMVKSISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAADGTGDF 185
           NQLTSQ+Y+LLSMVKSI NQP     SEFP+WLK+E+Q+LLQ ND AADATVAADGTGDF
Sbjct: 219 NQLTSQLYDLLSMVKSIPNQP-----SEFPSWLKSEDQNLLQINDLAADATVAADGTGDF 278

Query: 186 TTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFID 245
           T VM+AVLAAPD S+RRYVIY+KKGVY ENVEIKKKKWN+MMIGDGIDAT+ISGNRSFID
Sbjct: 279 TNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFID 338

Query: 246 GWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLY 305
           GWTTFRSATFAVSGRGFIARDITFENTAG EKHQAVALRSDSDLSVFFRCR+ GYQDTLY
Sbjct: 339 GWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLY 398

Query: 306 THTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTGF 365
           THTMRQFYREC ISGTVDFLFGDATVVFQNC+ILAKKGLPNQKNTITAQGRKDPNQPTGF
Sbjct: 399 THTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGF 458

Query: 366 SIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFALN 425
           SIQFCNISADSDL+PSVN TATYLGRPWKEYSRTIIMQSYIS+AIRPEGWLEWN +FALN
Sbjct: 459 SIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALN 518

Query: 426 TLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSGF 485
           TLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTV+QFIEGNLWLPSTGVK+T+G 
Sbjct: 519 TLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 576

Query: 486 GAN 489
           GAN
Sbjct: 579 GAN 576

BLAST of Carg01161 vs. TAIR 10
Match: AT4G33220.1 (pectin methylesterase 44 )

HSP 1 Score: 667.5 bits (1721), Expect = 8.0e-192
Identity = 326/499 (65.33%), Postives = 390/499 (78.16%), Query Frame = 0

Query: 9   QTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQL 68
           Q + LKVP+SEF +S+ + + V+RQV  I S F  F GD RL  A+SDCLDLLD SS++L
Sbjct: 24  QYDYLKVPASEFVSSINTIVVVIRQVSSILSQFADFSGDRRLQNAVSDCLDLLDFSSEEL 83

Query: 69  SWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQL 128
           +WS SA++NPK K N TGD+ SD +TWLSAA  N  TCMEGF+GT+ +VK LV+  ++QL
Sbjct: 84  TWSASASENPKGKGNGTGDVGSDTRTWLSAALSNQATCMEGFDGTSGLVKSLVAGSLDQL 143

Query: 129 TSQVYNLLSMV------KSISNQ-PAGKGES----------------EFPAWLKTEEQSL 188
            S +  LL +V      K++S   P  KG                  +FP W++ +++ L
Sbjct: 144 YSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLRDTDEDESLQFPDWVRPDDRKL 203

Query: 189 LQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNI 248
           L+ N    D +VA DGTG+FT +M+A+  APD S  R+VIY+KKG+Y ENVEIKKKKWNI
Sbjct: 204 LESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNI 263

Query: 249 MMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRS 308
           +M+GDGID TVISGNRSFIDGWTTFRSATFAVSGRGF+ARDITF+NTAGPEKHQAVALRS
Sbjct: 264 VMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRS 323

Query: 309 DSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLP 368
           DSDLSVFFRC M GYQDTLYTHTMRQFYREC I+GTVDF+FGD TVVFQNC ILAK+GLP
Sbjct: 324 DSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLP 383

Query: 369 NQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSY 428
           NQKNTITAQGRKD NQP+GFSIQF NISAD+DL P +N T TYLGRPWK YSRT+ +++ 
Sbjct: 384 NQKNTITAQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNN 443

Query: 429 ISNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVS 485
           +S+ +RPEGWLEWN DFAL+TLFY EFMNYGPG+GL+ RV WPGYH  N + +A NFTVS
Sbjct: 444 MSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVS 503

BLAST of Carg01161 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 666.4 bits (1718), Expect = 1.8e-191
Identity = 321/494 (64.98%), Postives = 401/494 (81.17%), Query Frame = 0

Query: 4   AADLEQTECLKVPSSEFSNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDS 63
           + DL Q ECL+VP  EF+ +  + +  + + + I S F K  G SR+S AI DC+DLLDS
Sbjct: 31  STDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKAGKSRVSNAIVDCVDLLDS 90

Query: 64  SSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSE 123
           ++++LSW +SA+Q+P  K+NSTGD+ SDL+TW+SAA  N +TC++GFEGTN I+K +V+ 
Sbjct: 91  AAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFEGTNGIIKKIVAG 150

Query: 124 GINQLTSQVYNLLSMVKSISNQP------------AGKGESEFPAWLKTEEQSLLQ-QND 183
           G++++ + V NLL+MV S  ++P            A  G S+FP+W+K  ++ LLQ  N 
Sbjct: 151 GLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSWVKPGDRKLLQTDNI 210

Query: 184 FAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFENVEIKKKKWNIMMIGD 243
             ADA VAADGTG+FTT+ +AVLAAPD S +RYVI+VK+GVY ENVEIKKKKWNIMM+GD
Sbjct: 211 TVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGD 270

Query: 244 GIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLS 303
           GIDATVI+GNRSFIDGWTTFRSATFAVSGRGFIARDITF+NTAGPEKHQAVA+RSD+DL 
Sbjct: 271 GIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLG 330

Query: 304 VFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQNCTILAKKGLPNQKNT 363
           VF+RC M GYQDTLY H+MRQF+REC I+GTVDF+FGDAT VFQ+C I AK+GLPNQKN+
Sbjct: 331 VFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNS 390

Query: 364 ITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKEYSRTIIMQSYISNAI 423
           ITAQGRKDPN+PTGF+IQF NI+AD+DL  ++N TATYLGRPWK YSRT+ MQ+Y+S+AI
Sbjct: 391 ITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAI 450

Query: 424 RPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVSQFIEG 483
            P GWLEWNG+FAL+TL+Y E+MN GPGA L +RV WPGYH LN ++EA NFTVSQ I+G
Sbjct: 451 NPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQG 510

Query: 484 NLWLPSTGVKFTSG 485
           NLWLPSTG+ F +G
Sbjct: 511 NLWLPSTGITFIAG 524

BLAST of Carg01161 vs. TAIR 10
Match: AT3G05620.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 436.0 bits (1120), Expect = 3.9e-122
Identity = 228/452 (50.44%), Postives = 299/452 (66.15%), Query Frame = 0

Query: 48  SRLSTAISDCLDLLDSSSDQLSWSL--------SAAQNPKAKNNSTGDLSSDLKTWLSAA 107
           SR   AI DC +L+  S  +L+WS+            +    ++       +LKTWLSAA
Sbjct: 99  SREQVAIEDCKELVGFSVTELAWSMLEMNKLHGGGGIDLDDGSHDAAAAGGNLKTWLSAA 158

Query: 108 GVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQP--AGKGESEF--P 167
             N +TC+EGFEGT    + L+   + Q+T  V N+L M   ++  P  A + ES    P
Sbjct: 159 MSNQDTCLEGFEGTERKYEELIKGSLRQVTQLVSNVLDMYTQLNALPFKASRNESVIASP 218

Query: 168 AWLKTEEQSLLQQND---FAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVY 227
            WL   ++SL+ ++D      +  VA DG G + T+ EA+  AP+ S +RYVIYVKKGVY
Sbjct: 219 EWLTETDESLMMRHDPSVMHPNTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVY 278

Query: 228 FENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT 287
            EN+++KKKK NIM++GDGI  T+I+G+R+F+ G TTFR+AT AVSGRGFIA+DITF NT
Sbjct: 279 KENIDLKKKKTNIMLVGDGIGQTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNT 338

Query: 288 AGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVV 347
           AGP+  QAVALR DSD S F+RC + GYQDTLY H++RQFYR+C I GT+DF+FG+   V
Sbjct: 339 AGPQNRQAVALRVDSDQSAFYRCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAV 398

Query: 348 FQNCTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRP 407
            QNC I  +  LP QK TITAQGRK PNQ TGF IQ   + A    QP      TYLGRP
Sbjct: 399 LQNCKIYTRVPLPLQKVTITAQGRKSPNQNTGFVIQNSYVLA---TQP------TYLGRP 458

Query: 408 WKEYSRTIIMQSYISNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR 467
           WK YSRT+ M +Y+S  ++P GWLEW G+FAL+TL+Y E+ N GPG   + RV WPGYH 
Sbjct: 459 WKLYSRTVYMNTYMSQLVQPRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYHI 518

Query: 468 LNQTSEATNFTVSQFIEGNLWLPSTGVKFTSG 485
           +++ + A +FTV  FI+G  WLP+TGV FT+G
Sbjct: 519 MDKRT-ALSFTVGSFIDGRRWLPATGVTFTAG 540

BLAST of Carg01161 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 427.6 bits (1098), Expect = 1.4e-119
Identity = 225/454 (49.56%), Postives = 296/454 (65.20%), Query Frame = 0

Query: 49  RLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTWLSAAGVNPETCME 108
           R  +A   C++LLD S D LS +LS+  +  AK         D+ TWLSAA  N +TC E
Sbjct: 149 RARSAYDSCVELLDDSVDALSRALSSVVSSSAK-------PQDVTTWLSAALTNHDTCTE 208

Query: 109 GFEGT-NSIVKGLVSEGINQLTSQVYNLLSMVKS-----------ISNQ---PAGKGESE 168
           GF+G  +  VK  ++  +  L+  V N L++  +           I N+      + E +
Sbjct: 209 GFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEEREEK 268

Query: 169 FPAWLKTEEQSLLQQ--NDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGV 228
           FP W++ +E+ +L+   +   AD  V+ DG G   T+ EA+  AP  S RR +IYVK G 
Sbjct: 269 FPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGR 328

Query: 229 YFE-NVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE 288
           Y E N+++ +KK N+M +GDG   TVISG +S  D  TTF +A+FA +G GFIARDITFE
Sbjct: 329 YEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFE 388

Query: 289 NTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDAT 348
           N AGP KHQAVALR  +D +V +RC + GYQDTLY H+ RQF+REC I GTVDF+FG+A 
Sbjct: 389 NWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAA 448

Query: 349 VVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLG 408
           VV QNC+I A+K +  QKNTITAQ RKDPNQ TG SI    + A SDLQ +  +T TYLG
Sbjct: 449 VVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLG 508

Query: 409 RPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGY 468
           RPWK +SRT+ M SYI   +   GWLEWN  FAL+TL+Y E++N GPG+GL +RV+WPGY
Sbjct: 509 RPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGY 568

Query: 469 HRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSG 485
             +N T+EA  FTV++FI G+ WLPSTGV F +G
Sbjct: 569 RVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAG 595

BLAST of Carg01161 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 424.5 bits (1090), Expect = 1.2e-118
Identity = 231/455 (50.77%), Postives = 295/455 (64.84%), Query Frame = 0

Query: 49  RLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLS-SDLKTWLSAAGVNPETCM 108
           R+ +A   CL+LLD S D L+ +LS+          +GD S SD+ TWLS+A  N +TC 
Sbjct: 136 RVRSAYDSCLELLDDSVDALTRALSSVV------VVSGDESHSDVMTWLSSAMTNHDTCT 195

Query: 109 EGF---EGTNSIVKGLVSEGINQLTSQVYNLLSM----VKSISNQPA-------GKGES- 168
           +GF   EG    VK  V   +  L+  V N L++    VK +S  P        G  E+ 
Sbjct: 196 DGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETE 255

Query: 169 EFPAWLKTEEQSLL--QQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKG 228
           E P WLK E++ LL    +   AD TV+ DG+G F T+ EA+  AP+ S RR+VIYVK G
Sbjct: 256 ELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAG 315

Query: 229 VY-FENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITF 288
            Y  EN+++ +KK N+M IGDG   TVI+G +S  D  TTF +ATFA +G GFI RD+TF
Sbjct: 316 RYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTF 375

Query: 289 ENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHISGTVDFLFGDA 348
           EN AGP KHQAVALR   D +V +RC + GYQD LY H+ RQF+REC I GTVDF+FG+A
Sbjct: 376 ENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNA 435

Query: 349 TVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYL 408
            V+ Q+C I A+K +  QK TITAQ RKDPNQ TG SI  C + A  DL+ S  +  TYL
Sbjct: 436 AVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYL 495

Query: 409 GRPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPG 468
           GRPWK YSR + M S + + I P GWLEWNG FAL++L+Y E+MN G G+G+ +RV WPG
Sbjct: 496 GRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPG 555

Query: 469 YHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSG 485
           YH +  T EA+ FTV+QFI G+ WLPSTGV F SG
Sbjct: 556 YHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSG 584