CSPI07G14130 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G14130
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	3-methyl-2-oxobutanoate hydroxymethyltransferase
Location	Chr7: 12609624 .. 12614977 (-)
RNA-Seq Expression	CSPI07G14130
Synteny	CSPI07G14130

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATGCTTGATGCTCTACGAAGTATACGAGGGTATCTTTCTCAAGTTTCAACCGTTTGTTCGTGATTTAGTTTGAGTAACGTTGGTCTTTCAATGGCGCTCTTCAAGCTTTTAACTATGGCCGTATCTCGAACAAGAACACAATCCCTCCTCAAAACCCTTCGTTACATGAGCAATGTCCCTGAAAATACGGTCTACGGCGGCCCCACCTCCCAAACTCCAAATCAAAGAGTCACTCTCACCAATCTTAGACAAAAGTACAAGAAAGGTGAACCCATCACCATGGTCACTGCTTATGATTACCCTTCCGGTGTCCATTTGGACATGGCTGGGATTGATATTGCTTTGGTAGGCGATTCGGCTGCCATGGTTGTGCACGGCCACGACACTACGTTGCCGATTAGTTTGGATGAAATCCTTGTTCATTGTAGGGCTGTGGCTCGTGGGGCTCGGCGGCCTCTTTTGATTGGTGATCTGCCCTTTGGTACTTATGAATCCAGCTCTGCACAGGTTTTTCTTTCTCTTTTACCTTTTAGGTGTTTGAAGAAATGACAAAGTTAGAGAATGTTTGTTTGTCTGTTGTCGCTTGAATGGGCTGTACGCGGAATTTAAGGGATTTGTGAATATAAAGGATTGAAAATGGTAAGTCTTAAATTCATATGAAAATATGTTTTCATTTAGTTTCTGGTTAGATTACGCCATCATAATGTCTCAAAATGCATAAACAGACCCAACTTACTTTTTGTTGGTATTTTGTTGCTTTTTGTTTTTACGACCATCACTTGATGTAAAGTAAATGTTTCACTTTTCCACTTTTATACTTCCTGTTCTTTTGTGACTCCTTTCAAGTGGGGTTTCTACCCTTTTCTTTGTTATGTAATTTCATATCACCAATAAAACTGATTCTCATACTAATTAATAAAGAAAAAAAAAAAAGAACATAAACTTTAAACGAACATATCTTGTGGAGTAATTATTTTGATAACTCGCTAGTTTAGAAATGTACATATTTGATGGGAAACTAGGTGGGATTGGTAGCTGTAATTGAATCGTACAAGCAACTTTTTTTAGGATGTCAAAACCTTAAACAAATCAGCAACTTCTAGTGGAGGAGGTAGAGCCTGAGGCTAAAGGCAATGTGGCGTGTGGGTGTAAATTGCATTCATGGAAGGGCTTGGGTAGAGAAAATCCCGGTAGAGGATCGACTGATATTTGAGTTTTAGGCATATTATGGAACGAATACACAGTTTCACGGAACACCATGTCTCTTGAGATGAAGATCTTTTTGTGCTCGATGTCATACAACTTGTAAAGCCTTCATGCCTTGGGGATAGCCAATAGCCAGCATGAGTTTGTATTGAAAATGGTCCCCAAATACAGCGAATTAAATCAAATGCATTTAAGACAAGTGATTATTGGAAGTAAAAGATAATTTCCTTCGTTTTTCCAAAGGACACACTGTACAATGAGATACTTTGTTTACTGTAGTGGATTGCAAATGTAAAAGGTTTCGTAATGAGTTTAATCAATCAGCAGAGGAATGGTCCAGTCGTTGATGCCATAAGGATGCTTTATTACCAACAATAGCACAAACGGATGTATTTGCAGGAAAGTCTGTGGGAGGGTCAAGAATGTATAGTCCTTGGAAAAGTTTAGCACTGCCAATCCTCTTCAAAGAGTACTTGTCCGGAATGATGCAAGTGTTGGTAGAAAATTGAACAAAAACTGATGAATCCTTTGTAATGGCACTCACTGAGAGCAGATTGACGGAAAAATCAAGCATATATAGAACATTTCTCAAAAGAAGTGTGTTAGATAATTTCACAGTACTGCTAGTGCTTTTTGACCACATAGGTGTTTTTATTTGGCAAACTAATGAATGCAGAACATGGTTCTTTGTCAGTAAAGAATTCTTCAGAACAACATATGCGTGTGGATGCTCCTGAGTCAATTATACAATATGGGGCAAAGAGAGTACCTGCTATGTGAGTGGTAGAACTACTTGAAGAGGCAGTAAATTATACAATATAGGGCCTCTGTTGGATTGTGATGTTGTTCAGATTGGGAGAGAGCTCACTGGTCTGTGTTATTCCCGAATTTGTACTGTTGGTGGAGGAAGTGCTCGAGTTACTGTTGTTGAGTCGCTGCTGTTTGGGCTTATATCCAGGAGGATAGCCATAGGGACTTTGTAACACTTGTAATTGGTATGTATTGAGATGTTACAATGAGTGCAAATAGGCCAATCTTTGCATTGTTTATGTGTAGAGTTGTTTTTCACGTTGTTTGAAGAGACAACAAGTGCGACGGCTGGAGCAACAGAAGAAAAAGAACTTATTGCTTGTTGTTCTTCTTCTTGAAGAAAAAGGGAGAATGCTTGAGAAACGGAAGGAATAGGTTCCATGAGGAGGAGTTGAGCACAGGCTTAAGCCAAAGACGCATTACGTCCCGTGAAGAAGTCCGTAAGTTATCCAATTTGAAGAAAATTAGTGATCTCTTGATTTCCTCCACATCGGCAATTTCCACGAGTACAAGTTGGCCTATAGGAGTTTAATTCACAACGAGAGTTTAAACTTTGTGAAATACATACTCACAGAGTCTTGATTTTGAGCGAGGGTTGCGAGAGATCTCTTCAACTGGAAAATGCAAGGAGCATTCTTTCGTTGAAAACACTCTTTGAAATCAAGCCATATACTTCTTACCGAATCTGTGAAGAAGATGCTGGCTGAAATCAGCTAGGATACTTAGTTCAAGATCCAGGATATGACGATGTTTTTGTTTCTGATCCATGAGGGAAGGAGATTGTCATTTGGCCGAGGAAGAGTTCCATCGACAAATCCGAATTTGTTTTGACTAATAGTCTGATGATCATCACACGCCTCCAGGAAACGTAGTTTTCTTCTGTTAACAGCTCAGTGACTAACACTAAACTTGTGTTGTCGTTATGGGGCAGATAATATGGATTGAGATTTCCTTGATTCTGATCAATTGAATGTTGAGGGCCATAAGATGTAGGAGGAACGTTTTCTTGAGTTTTAGAGGAGGTGGACAGAATCGGGTTTGCAGTTTGGGGGTTCTCGGTACGCATAGTGGTTTTGGTAGGGAAAGGTGGTTGAGAGGGATCAAAGTAAGGAGAAGTCACTCAGGAGGGAAAGTATGCTTTGGTAACGTATTGGAAGCTAAAAAGAATTCTAAATGACTTTCTCGCTTTATTGAATGGCTGTCCAATGATATACACATGTGAGAAGGGTAAATAAGTAGTTACCACGATAAGAGATGTTTAACTAACTAACGAATTCTAGACCTCTTAACTAATTGTTTAATAACTTCTCACTTGATGAACATCTCCAATTCTTAAAATGATCCAGCACTGTGTTGTATTATTGACAGGCTATTGATACGGCAGTGAGAATTCTAAAGGAAGGGGGAATGGATGCGATAAAATTGGAAGGGGGTTCCCATTCTAGAATAACAGCAGCAAGAGCTATAGTTGAAGCAGGGATTGCGGTAATGGGGCATGTTGGACTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAAGAATATCACCAGTGCTGTTAAGGTTCCTCCACTTCTCTACTTATCTCTTCCTCTTATTGTACTGTTTGCAGCTATTTCTTGGACACCATTCTGTGGTCATGTTGCATAGTTTTTTGTGTTTATTTCAGCCTTTATGCTGAATATGCATCTTTGCAGGTGGTGGAAACTGCAATCTCTCTACAAGAAGCTGGGTGTTTTGCTGTCGTCCTAGAATGTGTTCCTCCAGAAGTTGCTGCTGCGGCTACAGCAGCTCTCCGTATTCCCACCATGGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTTAGTTGATTGTTTGTTAATATTTCCTTTCCTTAACGTATTTCCTTTCGTTGAGCTGTTTGTTTGTCTCTCGTTTGGTCTGGCATATAATTAATAACTTCAAATTATTTCTTTACCTAGTTCAGGATTTGGAGGTTCTCTCCTGAATAAGAGAAGCATCAAAAGGTTGCCATGTTTAGTACTCCAAAGTGGTCCTCTTTTATGGTTTTTCAGTTTTGGTACAATGTAATTGGAAGTGAATGGTATAACTATTTTAAGTGTTTCAAGATCCCCTAAAGGGAGTGGATGCATCAGAATATAGAAGACATTCAAGAGTAGTGAAATTTTTTTTTCCGGTCAACAGTATACTCACTAACAAGTGGAAATGAAATAACAATTTGTTAATATTTGGGTTTGAATGCCACTCTCGTATTGAAATGAGAATGTGAAGAAGAAAATGGACATTGCATTTCAGTCAAGAGACAGGGTTTATGATCTATTTAAATATTTCAGACTACTTGTTGGTTTTTTATTTTTTGTTCTTTTAAATAGAAATGTCGGTTTCATCTTTCATACTTGTTTGATAAGGTTGATTGGTGTGTTGCTCATGTTGTACTTGATTACTTGACAACAGTCATAGTGTCGACTCAAATGTCCTTTGCAGTTGGACTAATTAATTTTCTTAAACTCAGGTCCTAGTTTACCATGATCTGCTCGGCATGCTTCAACACCCACATCATGCTAAGGTTATCAGACTCCACCTTTGTTTTGTATAGGTTTCTCCATGTTTTTTTTATGATGTTATGACTTCTTTTGTTTATGCCCTTTGCAGGTGACCCCGAAATTTTGTAAGCAATTTGCTCAAATTGGAGATGTCATCAACAAAGCTTTATTAGAATATAGGGAAGAAGTATCTAATGGTTCGTTTCCTGGACCGCTTCATAGCCCATATAAGATCAGCGACGCTGATTTTAATGGTTTCTTGAAAGAGTTACAAAATATGGGATTGGATGAGGCAGCATCAGCAGCTGCTGCTAAAGCCAACCGATGATTAAACTCCGGCCACATTAAATTGGCCCGCCTTCCTTGGTCTTGCTTTTTCCAAGTTTTAAATAATTCAGGGAATTTATAAGGTGGGACAATTTGTTCAAAGAATCAGTAGTTGAAAAATGCTTCAAACTCTTATATATAAGGATCGCAATCAACGTTTTCTTTAGGCAACCAGTTCCTTCTTAGATTTCCTCCATGATGATCCCTTTTTGTTAGTTAGCTGATATGAAGATGTGTTTGGATTCTGAACAAAATGAAAGTATCCAAAGAAAACGGAAAAGGAAGCAAAGCCACAGCCGCATGGTGACTCTTTTTCTAACCAAATATTTTAGTGTTTAGTTGTGGAATAAGCAAGCAAAAAGGGATATACCTATCTAAACTTAGGTTGTATTTTTTGCTTGCAATAAATAATAAGGGTTGAAGTTCTTTGTATTTGAAATTTGCTTGGTGG

mRNA sequence

CAATGCTTGATGCTCTACGAAGTATACGAGGGTATCTTTCTCAAGTTTCAACCGTTTGTTCGTGATTTAGTTTGAGTAACGTTGGTCTTTCAATGGCGCTCTTCAAGCTTTTAACTATGGCCGTATCTCGAACAAGAACACAATCCCTCCTCAAAACCCTTCGTTACATGAGCAATGTCCCTGAAAATACGGTCTACGGCGGCCCCACCTCCCAAACTCCAAATCAAAGAGTCACTCTCACCAATCTTAGACAAAAGTACAAGAAAGGTGAACCCATCACCATGGTCACTGCTTATGATTACCCTTCCGGTGTCCATTTGGACATGGCTGGGATTGATATTGCTTTGGTAGGCGATTCGGCTGCCATGGTTGTGCACGGCCACGACACTACGTTGCCGATTAGTTTGGATGAAATCCTTGTTCATTGTAGGGCTGTGGCTCGTGGGGCTCGGCGGCCTCTTTTGATTGGTGATCTGCCCTTTGGTACTTATGAATCCAGCTCTGCACAGGCTATTGATACGGCAGTGAGAATTCTAAAGGAAGGGGGAATGGATGCGATAAAATTGGAAGGGGGTTCCCATTCTAGAATAACAGCAGCAAGAGCTATAGTTGAAGCAGGGATTGCGGTAATGGGGCATGTTGGACTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAAGAATATCACCAGTGCTGTTAAGGTGGTGGAAACTGCAATCTCTCTACAAGAAGCTGGGTGTTTTGCTGTCGTCCTAGAATGTGTTCCTCCAGAAGTTGCTGCTGCGGCTACAGCAGCTCTCCGTATTCCCACCATGGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTCCTAGTTTACCATGATCTGCTCGGCATGCTTCAACACCCACATCATGCTAAGGTGACCCCGAAATTTTGTAAGCAATTTGCTCAAATTGGAGATGTCATCAACAAAGCTTTATTAGAATATAGGGAAGAAGTATCTAATGGTTCGTTTCCTGGACCGCTTCATAGCCCATATAAGATCAGCGACGCTGATTTTAATGGTTTCTTGAAAGAGTTACAAAATATGGGATTGGATGAGGCAGCATCAGCAGCTGCTGCTAAAGCCAACCGATGATTAAACTCCGGCCACATTAAATTGGCCCGCCTTCCTTGGTCTTGCTTTTTCCAAGTTTTAAATAATTCAGGGAATTTATAAGGTGGGACAATTTGTTCAAAGAATCAGTAGTTGAAAAATGCTTCAAACTCTTATATATAAGGATCGCAATCAACGTTTTCTTTAGGCAACCAGTTCCTTCTTAGATTTCCTCCATGATGATCCCTTTTTGTTAGTTAGCTGATATGAAGATGTGTTTGGATTCTGAACAAAATGAAAGTATCCAAAGAAAACGGAAAAGGAAGCAAAGCCACAGCCGCATGGTGACTCTTTTTCTAACCAAATATTTTAGTGTTTAGTTGTGGAATAAGCAAGCAAAAAGGGATATACCTATCTAAACTTAGGTTGTATTTTTTGCTTGCAATAAATAATAAGGGTTGAAGTTCTTTGTATTTGAAATTTGCTTGGTGG

Coding sequence (CDS)

ATGGCGCTCTTCAAGCTTTTAACTATGGCCGTATCTCGAACAAGAACACAATCCCTCCTCAAAACCCTTCGTTACATGAGCAATGTCCCTGAAAATACGGTCTACGGCGGCCCCACCTCCCAAACTCCAAATCAAAGAGTCACTCTCACCAATCTTAGACAAAAGTACAAGAAAGGTGAACCCATCACCATGGTCACTGCTTATGATTACCCTTCCGGTGTCCATTTGGACATGGCTGGGATTGATATTGCTTTGGTAGGCGATTCGGCTGCCATGGTTGTGCACGGCCACGACACTACGTTGCCGATTAGTTTGGATGAAATCCTTGTTCATTGTAGGGCTGTGGCTCGTGGGGCTCGGCGGCCTCTTTTGATTGGTGATCTGCCCTTTGGTACTTATGAATCCAGCTCTGCACAGGCTATTGATACGGCAGTGAGAATTCTAAAGGAAGGGGGAATGGATGCGATAAAATTGGAAGGGGGTTCCCATTCTAGAATAACAGCAGCAAGAGCTATAGTTGAAGCAGGGATTGCGGTAATGGGGCATGTTGGACTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAAGAATATCACCAGTGCTGTTAAGGTGGTGGAAACTGCAATCTCTCTACAAGAAGCTGGGTGTTTTGCTGTCGTCCTAGAATGTGTTCCTCCAGAAGTTGCTGCTGCGGCTACAGCAGCTCTCCGTATTCCCACCATGGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTCCTAGTTTACCATGATCTGCTCGGCATGCTTCAACACCCACATCATGCTAAGGTGACCCCGAAATTTTGTAAGCAATTTGCTCAAATTGGAGATGTCATCAACAAAGCTTTATTAGAATATAGGGAAGAAGTATCTAATGGTTCGTTTCCTGGACCGCTTCATAGCCCATATAAGATCAGCGACGCTGATTTTAATGGTTTCTTGAAAGAGTTACAAAATATGGGATTGGATGAGGCAGCATCAGCAGCTGCTGCTAAAGCCAACCGATGA

Protein sequence

MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGEPITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYREEVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR*

Homology

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BLAST of CSPI07G14130 vs. ExPASy Swiss-Prot
Match: Q9M315 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=KPHMT2 PE=1 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 6.4e-156
Identity = 271/334 (81.14%), Postives = 305/334 (91.32%), Query Frame = 0


Query: 14  TRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGEPITMVTAYDYPSG 73
           +R    + T+R+MSNVPE+TVYGGP  Q  NQRVTLT LRQK++KGEPITMVTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFGTY 133
           VH+D AGID+ LVGDSAAMVVHG+DTTLPISL+E+LVHCRAVARGA+RPLL+GDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QA+DTAVR+LKEGGMDAIKLEGGS SRITAA++IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPF 253
           GGFRPQGKNI SAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL IPT+GIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYREEVSNGSFPGPLHS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+AQ+G+VINKALLEY+EEVS   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISDADFNGFLKELQNMGLDEAASAAAAKANR 348
           PYKIS +D +GFL ELQ +GLD+AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

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BLAST of CSPI07G14130 vs. ExPASy Swiss-Prot
Match: O82357 (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=KPHMT1 PE=1 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 2.1e-154
Identity = 271/340 (79.71%), Postives = 309/340 (90.88%), Query Frame = 0


Query: 9   MAVSRTRTQSLLK---TLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGEPITMV 68
           MA S TR  S      ++R+MSN+PENTVYGGP  Q PNQRVTLT+LRQK+++GEPIT+V
Sbjct: 1   MASSLTRNCSRFSKAISVRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVV 60

Query: 69  TAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLI 128
           TAYDYPS VHLD AGID+ LVGDSA+MVVHGHDTTLPISLDE+LVHCRAVARGA+RPLL+
Sbjct: 61  TAYDYPSAVHLDTAGIDVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLV 120

Query: 129 GDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVMGHVGL 188
           GDLPFGTYESSS+QA+DTAVR+LKEGGMDAIKLEGGS SRITAA+AIVEAGIAV+GHVGL
Sbjct: 121 GDLPFGTYESSSSQAVDTAVRVLKEGGMDAIKLEGGSASRITAAKAIVEAGIAVIGHVGL 180

Query: 189 TPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPT 248
           TPQAISVLGGFRPQG+NI SAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL+IPT
Sbjct: 181 TPQAISVLGGFRPQGRNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALKIPT 240

Query: 249 MGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYREEVSNG 308
           +GIGAGPFCSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+A +G+VINKAL+EY+EEVS  
Sbjct: 241 IGIGAGPFCSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYANVGEVINKALMEYKEEVSKK 300

Query: 309 SFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKA 346
            FPGP HSPYKI+ ++ +GFL ELQ +G D+AASAAA  A
Sbjct: 301 VFPGPSHSPYKITASELDGFLTELQKLGFDKAASAAALAA 340

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BLAST of CSPI07G14130 vs. ExPASy Swiss-Prot
Match: Q9AWZ8 (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=KPHMT1 PE=2 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 8.4e-140
Identity = 250/334 (74.85%), Postives = 285/334 (85.33%), Query Frame = 0


Query: 24  RYMSNVPENTVYGGPTSQT------------PNQRVTLTNLRQKYKKGEPITMVTAYDYP 83
           R +S+VPE+ VYGGP  Q               +RVT+T LR K+++GEPIT+VTAYDYP
Sbjct: 18  RPLSHVPESAVYGGPRPQDVGAAAGAGAGAGATRRVTVTTLRGKHRRGEPITVVTAYDYP 77

Query: 84  SGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFG 143
           S VH+D AGID+ LVGDSAAMVVHGHDTTLPISLD +L HCRAVARGA RPLL+GDLPFG
Sbjct: 78  SAVHVDSAGIDVCLVGDSAAMVVHGHDTTLPISLDVMLEHCRAVARGATRPLLVGDLPFG 137

Query: 144 TYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVMGHVGLTPQAIS 203
            YESSS +A+D+AVR+LKEGGMDAIKLEGG+ SRI+AA+AIVEAGIAVMGHVGLTPQAIS
Sbjct: 138 CYESSSTRAVDSAVRVLKEGGMDAIKLEGGAPSRISAAKAIVEAGIAVMGHVGLTPQAIS 197

Query: 204 VLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAG 263
           VLGGFRPQGK + SAVKVVETA++LQEAGCF+VVLECVP  VAAAAT+AL+IPT+GIGAG
Sbjct: 198 VLGGFRPQGKTVDSAVKVVETALALQEAGCFSVVLECVPAPVAAAATSALQIPTIGIGAG 257

Query: 264 PFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYREEVSNGSFPGPL 323
           PFCSGQVLVYHDLLGM+QHPHHAKVTPKFCKQF  +G VINKAL EY++EV   SFPGP 
Sbjct: 258 PFCSGQVLVYHDLLGMMQHPHHAKVTPKFCKQFGNVGHVINKALSEYKQEVETRSFPGPS 317

Query: 324 HSPYKISDADFNGFLKELQNMGLDEAASAAAAKA 346
           H+PYKI+ AD +GF   LQ MGLDEAA+AAAA A
Sbjct: 318 HTPYKIAAADVDGFANALQKMGLDEAANAAAAAA 351

[Close]

BLAST of CSPI07G14130 vs. ExPASy Swiss-Prot
Match: Q9AWZ7 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=KPHMT2 PE=3 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 2.5e-120
Identity = 220/326 (67.48%), Postives = 263/326 (80.67%), Query Frame = 0


Query: 24  RYMSN-VPENTVYGG------PTSQTPNQRVTLTNLRQKYKKGEPITMVTAYDYPSGVHL 83
           R MSN   E  +YGG        S    +RVTL  LR K+++GEPI+MVTAYDYPSGVH+
Sbjct: 54  RAMSNGAAEPAIYGGGGGAQQAASSAAARRVTLATLRGKHRRGEPISMVTAYDYPSGVHV 113

Query: 84  DMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFGTYESS 143
           D AG DI LVGDSAAMV HGHD TLPISLD ++ HCRAVARGA R  L+GDLPFG+YE+S
Sbjct: 114 DAAGFDICLVGDSAAMVAHGHDNTLPISLDLMIEHCRAVARGAARTFLVGDLPFGSYEAS 173

Query: 144 SAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGF 203
           +AQA+ +AVR++KEGG+++IKLEG + SRI+AARAIV+AGIAVMGH+GLTPQ++S LGGF
Sbjct: 174 TAQAVGSAVRVMKEGGVNSIKLEGSAPSRISAARAIVDAGIAVMGHIGLTPQSVSALGGF 233

Query: 204 RPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPFCSG 263
           RPQGK + SAVKVVE A++LQEAGCFAVVLECVP  VAAAAT+AL IPT+GIGAGPFCSG
Sbjct: 234 RPQGKTVESAVKVVEAALALQEAGCFAVVLECVPAPVAAAATSALTIPTIGIGAGPFCSG 293

Query: 264 QVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYREEVSNGSFPGPLHSPYK 323
           QVLVYHDLLG  Q   HAKV+PKFCKQ+  IGDVIN+AL +Y++EV   SFPGP H+PYK
Sbjct: 294 QVLVYHDLLGTFQ-TSHAKVSPKFCKQYGNIGDVINRALSKYKQEVETQSFPGPSHTPYK 353

Query: 324 ISDADFNGFLKELQNMGLDEAASAAA 343
           ++  D + FL  L+  GL+ AA AAA
Sbjct: 354 LAATDVDAFLNALKMKGLNVAADAAA 378

[Close]

BLAST of CSPI07G14130 vs. ExPASy Swiss-Prot
Match: B0TC10 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) OX=498761 GN=panB PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.1e-70
Identity = 134/269 (49.81%), Postives = 191/269 (71.00%), Query Frame = 0


Query: 45  QRVTLTNLRQKYKKGEPITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPIS 104
           +++TL   ++  K+G+ + M+TAYDYP    +D + I+I LVGDS  MVV G+D+T+P++
Sbjct: 3   KKITLPQCKEMKKQGKRLRMITAYDYPFARLVDESEIEIILVGDSLGMVVLGYDSTVPVT 62

Query: 105 LDEILVHCRAVARGARRPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHS 164
           LDE++ HC+ V RGA   L++ D+PFG+Y  S   AI  A R+LKE G++A+K+EGG+  
Sbjct: 63  LDEMIHHCKPVVRGAPNTLVVADMPFGSYNVSKEDAIRNANRMLKESGIEAVKVEGGTRM 122

Query: 165 RITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAV 224
             T  RA+V+AGI VMGH+GLTPQ  + LGGF+ QGK   +A +++E A++L+ AG F++
Sbjct: 123 APT-VRALVDAGIPVMGHIGLTPQTAAQLGGFKVQGKTEDAAQQLLEDALALESAGAFSI 182

Query: 225 VLECVPPEVAAAATAALRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQF 284
           V+ECVP  +A   TA+L IPT+GIGAGP+C+GQVLV  DLLG+     + +  PKF KQ+
Sbjct: 183 VIECVPAGLARTITASLSIPTIGIGAGPYCNGQVLVIQDLLGI-----YDRFVPKFVKQY 242

Query: 285 AQIGDVINKALLEYREEVSNGSFPGPLHS 314
           AQ G  I  AL +Y  EV++G FPGP HS
Sbjct: 243 AQTGPAIRAALNDYAREVADGVFPGPEHS 265

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BLAST of CSPI07G14130 vs. ExPASy TrEMBL
Match: A0A0A0K8B2 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G338120 PE=3 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 8.3e-191
Identity = 346/347 (99.71%), Postives = 347/347 (100.00%), Query Frame = 0


Query: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60
           MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPI+LDEILVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR
Sbjct: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 347

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BLAST of CSPI07G14130 vs. ExPASy TrEMBL
Match: A0A1S3BH28 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103489751 PE=3 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 3.1e-185
Identity = 337/347 (97.12%), Postives = 339/347 (97.69%), Query Frame = 0


Query: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60
           MALFKLLTMA SRTR QSLLKTLRYMSNVPENTVY GPTSQTPNQRVTLTNLRQKYKKGE
Sbjct: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSSAQA+DTAVRILKEGGMDAIKLEGG+HSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 348
           EVSN SFPGP HSPYKISD D NGFLKELQNMGLDEAASAAAAKANR
Sbjct: 301 EVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 347

[Close]

BLAST of CSPI07G14130 vs. ExPASy TrEMBL
Match: A0A6J1JXR6 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111488459 PE=3 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.3e-177
Identity = 318/347 (91.64%), Postives = 335/347 (96.54%), Query Frame = 0


Query: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60
           MALF L+T A  R+ +QSLL TLRYMSNVPENTVYGGPTS TPNQRVTLTNL+QKYKKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSA+MVVHGHDTTLPI+LDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSS QA+DTAVR+LKEGGMDAIKLEGG+HSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKAL +Y+E
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGPLHSPYKISD D+NGFLKEL++MGLD AASAAAAKAN+
Sbjct: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 347

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BLAST of CSPI07G14130 vs. ExPASy TrEMBL
Match: A0A6J1GNW3 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456114 PE=3 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 2.0e-176
Identity = 317/347 (91.35%), Postives = 334/347 (96.25%), Query Frame = 0


Query: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60
           MALF L+T A  R+ +QSLL TLR MSNVPENTVYGGPTS TPNQRVTLTNL+QKYKKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSA+MVVHGHDTTLPI+LDE+LVHCR+VARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSS QA+DTAVR+LKEGGMDAIKLEGG+HSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKAL +Y+E
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGPLHSPYKISD D+NGFLKEL++MGLD AASAAAAKANR
Sbjct: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 347

[Close]

BLAST of CSPI07G14130 vs. ExPASy TrEMBL
Match: A0A1S4DXV9 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103491726 PE=3 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 7.5e-176
Identity = 317/346 (91.62%), Postives = 333/346 (96.24%), Query Frame = 0


Query: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60
           MA+FKLLT   S+TRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNL QKYKKGE
Sbjct: 1   MAVFKLLTWIASQTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLIQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGH++TLPISLDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHNSTLPISLDEMLVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180
           RP LI DLPFGTYESS  QA+DTAV+ILKEG MDAIKLEGG+HSRITAARAIV+AGIAVM
Sbjct: 121 RPFLIVDLPFGTYESSHVQAVDTAVKILKEGRMDAIKLEGGAHSRITAARAIVDAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAAT A
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATVA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLG+LQHPHHAKVTPKFCKQFAQIGDVIN+AL EYRE
Sbjct: 241 LRIPTLGIGAGPFCSGQVLVYHDLLGILQHPHHAKVTPKFCKQFAQIGDVINQALSEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKAN 347
           EVSNGSFPGPLH+PYKISDAD NGF+KELQNMGLDEAASAA+AKA+
Sbjct: 301 EVSNGSFPGPLHNPYKISDADLNGFMKELQNMGLDEAASAASAKAS 346

[Close]

BLAST of CSPI07G14130 vs. NCBI nr
Match: XP_004139651.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial [Cucumis sativus] >KGN44587.1 hypothetical protein Csa_016545 [Cucumis sativus])

HSP 1 Score: 676.0 bits (1743), Expect = 1.7e-190
Identity = 346/347 (99.71%), Postives = 347/347 (100.00%), Query Frame = 0


Query: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60
           MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPI+LDEILVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR
Sbjct: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 347

[Close]

BLAST of CSPI07G14130 vs. NCBI nr
Match: XP_008447278.1 (PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucumis melo])

HSP 1 Score: 657.5 bits (1695), Expect = 6.3e-185
Identity = 337/347 (97.12%), Postives = 339/347 (97.69%), Query Frame = 0


Query: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60
           MALFKLLTMA SRTR QSLLKTLRYMSNVPENTVY GPTSQTPNQRVTLTNLRQKYKKGE
Sbjct: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSSAQA+DTAVRILKEGGMDAIKLEGG+HSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 348
           EVSN SFPGP HSPYKISD D NGFLKELQNMGLDEAASAAAAKANR
Sbjct: 301 EVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 347

[Close]

BLAST of CSPI07G14130 vs. NCBI nr
Match: XP_038898896.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 642.5 bits (1656), Expect = 2.1e-180
Identity = 325/347 (93.66%), Postives = 335/347 (96.54%), Query Frame = 0


Query: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60
           MA+F L + A SRTRT+ LLKTLR MSNVPEN VYGGPTSQTPN RVTLTNLRQKYKKGE
Sbjct: 1   MAIFNLFSKAASRTRTRPLLKTLRNMSNVPENAVYGGPTSQTPNHRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHG+DTTLPISLDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGYDTTLPISLDEMLVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180
           RPLL+GDLPFGTYESSS QA+DTAVR+LKEGGMDAIKLEGG+HSRITAARAIVEAGIAVM
Sbjct: 121 RPLLVGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAAT+A
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATSA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKAL EYRE
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSEYRE 300

Query: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGPLHSPYKISD DFNGFLKELQNMGLDEAASAAAAKANR
Sbjct: 301 EVSNGSFPGPLHSPYKISDTDFNGFLKELQNMGLDEAASAAAAKANR 347

[Close]

BLAST of CSPI07G14130 vs. NCBI nr
Match: XP_022991968.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 631.3 bits (1627), Expect = 4.8e-177
Identity = 318/347 (91.64%), Postives = 335/347 (96.54%), Query Frame = 0


Query: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60
           MALF L+T A  R+ +QSLL TLRYMSNVPENTVYGGPTS TPNQRVTLTNL+QKYKKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSA+MVVHGHDTTLPI+LDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSS QA+DTAVR+LKEGGMDAIKLEGG+HSRITAARAIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKAL +Y+E
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGPLHSPYKISD D+NGFLKEL++MGLD AASAAAAKAN+
Sbjct: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 347

[Close]

BLAST of CSPI07G14130 vs. NCBI nr
Match: KAG6575974.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 628.2 bits (1619), Expect = 4.1e-176
Identity = 317/347 (91.35%), Postives = 334/347 (96.25%), Query Frame = 0


Query: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60
           MALF L+T A  R+ +QSLL TLR MSNVPENTVYGGPTS TPNQRVTLTNL+QKYKKGE
Sbjct: 117 MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 176

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSA+MVVHGHDTTLPI+LDE+LVHCR+VARGAR
Sbjct: 177 PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 236

Query: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180
           RPLLIGDLPFGTYESSS QA+DTAVR+LKEGGMDAIKLEGG+HSRITAARAIVEAGIAVM
Sbjct: 237 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 296

Query: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA
Sbjct: 297 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 356

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKAL +Y+E
Sbjct: 357 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 416

Query: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 348
           EVSNGSFPGPLHSPYKISD D+NGFLKEL++MGLD AASAAAAKANR
Sbjct: 417 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 463

[Close]

BLAST of CSPI07G14130 vs. TAIR 10
Match: AT3G61530.1 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 551.6 bits (1420), Expect = 4.5e-157
Identity = 271/334 (81.14%), Postives = 305/334 (91.32%), Query Frame = 0


Query: 14  TRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGEPITMVTAYDYPSG 73
           +R    + T+R+MSNVPE+TVYGGP  Q  NQRVTLT LRQK++KGEPITMVTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFGTY 133
           VH+D AGID+ LVGDSAAMVVHG+DTTLPISL+E+LVHCRAVARGA+RPLL+GDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QA+DTAVR+LKEGGMDAIKLEGGS SRITAA++IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPF 253
           GGFRPQGKNI SAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL IPT+GIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYREEVSNGSFPGPLHS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+AQ+G+VINKALLEY+EEVS   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISDADFNGFLKELQNMGLDEAASAAAAKANR 348
           PYKIS +D +GFL ELQ +GLD+AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

[Close]

BLAST of CSPI07G14130 vs. TAIR 10
Match: AT3G61530.2 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 551.6 bits (1420), Expect = 4.5e-157
Identity = 271/334 (81.14%), Postives = 305/334 (91.32%), Query Frame = 0


Query: 14  TRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGEPITMVTAYDYPSG 73
           +R    + T+R+MSNVPE+TVYGGP  Q  NQRVTLT LRQK++KGEPITMVTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFGTY 133
           VH+D AGID+ LVGDSAAMVVHG+DTTLPISL+E+LVHCRAVARGA+RPLL+GDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QA+DTAVR+LKEGGMDAIKLEGGS SRITAA++IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPF 253
           GGFRPQGKNI SAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL IPT+GIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYREEVSNGSFPGPLHS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+AQ+G+VINKALLEY+EEVS   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISDADFNGFLKELQNMGLDEAASAAAAKANR 348
           PYKIS +D +GFL ELQ +GLD+AAS AAA A +
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

[Close]

BLAST of CSPI07G14130 vs. TAIR 10
Match: AT2G46110.1 (ketopantoate hydroxymethyltransferase 1 )

HSP 1 Score: 546.6 bits (1407), Expect = 1.5e-155
Identity = 271/340 (79.71%), Postives = 309/340 (90.88%), Query Frame = 0


Query: 9   MAVSRTRTQSLLK---TLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGEPITMV 68
           MA S TR  S      ++R+MSN+PENTVYGGP  Q PNQRVTLT+LRQK+++GEPIT+V
Sbjct: 1   MASSLTRNCSRFSKAISVRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVV 60

Query: 69  TAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLI 128
           TAYDYPS VHLD AGID+ LVGDSA+MVVHGHDTTLPISLDE+LVHCRAVARGA+RPLL+
Sbjct: 61  TAYDYPSAVHLDTAGIDVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLV 120

Query: 129 GDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVMGHVGL 188
           GDLPFGTYESSS+QA+DTAVR+LKEGGMDAIKLEGGS SRITAA+AIVEAGIAV+GHVGL
Sbjct: 121 GDLPFGTYESSSSQAVDTAVRVLKEGGMDAIKLEGGSASRITAAKAIVEAGIAVIGHVGL 180

Query: 189 TPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPT 248
           TPQAISVLGGFRPQG+NI SAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL+IPT
Sbjct: 181 TPQAISVLGGFRPQGRNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALKIPT 240

Query: 249 MGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYREEVSNG 308
           +GIGAGPFCSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+A +G+VINKAL+EY+EEVS  
Sbjct: 241 IGIGAGPFCSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYANVGEVINKALMEYKEEVSKK 300

Query: 309 SFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKA 346
            FPGP HSPYKI+ ++ +GFL ELQ +G D+AASAAA  A
Sbjct: 301 VFPGPSHSPYKITASELDGFLTELQKLGFDKAASAAALAA 340

The following BLAST results are available for this feature:

BLAST of CSPI07G14130 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M315	6.4e-156	81.14	3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis...	[more]
O82357	2.1e-154	79.71	3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis...	[more]
Q9AWZ8	8.4e-140	74.85	3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativ...	[more]
Q9AWZ7	2.5e-120	67.48	3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativ...	[more]
B0TC10	1.1e-70	49.81	3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Heliobacterium modesticaldum...	[more]

BLAST of CSPI07G14130 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K8B2	8.3e-191	99.71	3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A1S3BH28	3.1e-185	97.12	3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A6J1JXR6	2.3e-177	91.64	3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1GNW3	2.0e-176	91.35	3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita moschata OX=3662 G...	[more]
A0A1S4DXV9	7.5e-176	91.62	3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC1...	[more]

BLAST of CSPI07G14130 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139651.1	1.7e-190	99.71	3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial [Cucumis sativ...	[more]
XP_008447278.1	6.3e-185	97.12	PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-lik...	[more]
XP_038898896.1	2.1e-180	93.66	3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Benincas...	[more]
XP_022991968.1	4.8e-177	91.64	3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbit...	[more]
KAG6575974.1	4.1e-176	91.35	3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial, partial [Cucu...	[more]

BLAST of CSPI07G14130 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G61530.1	4.5e-157	81.14	Phosphoenolpyruvate carboxylase family protein	[more]
AT3G61530.2	4.5e-157	81.14	Phosphoenolpyruvate carboxylase family protein	[more]
AT2G46110.1	1.5e-155	79.71	ketopantoate hydroxymethyltransferase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR040442	Pyruvate kinase-like domain superfamily	GENE3D	3.20.20.60		coord: 44..326 e-value: 1.6E-118 score: 396.9
IPR003700	Ketopantoate hydroxymethyltransferase	TIGRFAM	TIGR00222	TIGR00222	coord: 48..313 e-value: 1.4E-86 score: 288.3
IPR003700	Ketopantoate hydroxymethyltransferase	PFAM	PF02548	Pantoate_transf	coord: 46..309 e-value: 3.5E-105 score: 351.1
IPR003700	Ketopantoate hydroxymethyltransferase	PANTHER	PTHR20881	3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE	coord: 15..346
IPR003700	Ketopantoate hydroxymethyltransferase	HAMAP	MF_00156	PanB	coord: 47..314 score: 39.745171
IPR003700	Ketopantoate hydroxymethyltransferase	CDD	cd06557	KPHMT-like	coord: 49..308 e-value: 1.58276E-141 score: 399.101
None	No IPR available	PIRSR	PIRSR000388-1	PIRSR000388-1	coord: 55..309 e-value: 8.2E-88 score: 291.8
None	No IPR available	PANTHER	PTHR20881:SF1	3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE	coord: 15..346
IPR015813	Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily	SUPERFAMILY	51621	Phosphoenolpyruvate/pyruvate domain	coord: 46..312

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G14130.1	CSPI07G14130.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0015940	pantothenate biosynthetic process
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0003864	3-methyl-2-oxobutanoate hydroxymethyltransferase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003824	catalytic activity