CSPI06G20400 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G20400
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	TLC domain-containing protein 2
Location	Chr6: 18444581 .. 18448233 (+)
RNA-Seq Expression	CSPI06G20400
Synteny	CSPI06G20400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTTGCCCCTCTCCTCTCTCCGCTCTCTTCATTTCTGTTTATGTCAATCGTCCTCTTCTCCCGCTGATACGGAACACACGATTTAGCTCCACACCGACTCCATGGCGGGTAGAACATTGTTGAAGGAGAAAAGCAGGGCATGGAGCTTCTTCTCCGCGACATTACTGCTTTGGTCAGTCTCTCTTTGGTTCGAAATTCTATTCGACCGACGATTCCGGCTGCTCTACGTCGTTTTCGGAGCTCTGTTCTACCAGTCCGCCAATTCCTCCATTAGATTACTTCTTTCCAAGGATCCTCTTTTAGTCAACACCTCCGTTTCTCTTCTTCATTCCATTATCACCTCTGCTTCAGGTTCTTTGCTGCTCTTTTACCATCTCGTTGTTTGTTTTCTCTTTCTTTTCTCCTTCTTTTAATTCTGTTTATAACTTCGCTTGTTGTTTTTAATTTTCATCATATATGACTGCCTTCCTTGCTCACTGTTTTGATTCTTTCTCTGGAAGTTACTTCTTCGTCTTGCTTAGCTTGAAGGTTTTACTGATTGAAATAAGAAGCTTTATTTGCACTATAAGAAAGACTCAGAGTTGGTCTTGTTTCGTTTTTGCAAGTTCTGTGTAGTAAATTTTGTGAATTATTAGTTATTGATGCGGTTTAGTGCGCATGCTACAGTTGGTTGTTTGAGCCAATGAACCGAGCTCAAATGAATTATTTTGGACCGCTTTTACTGTGCCAAGAATGTCAAGACTTTGTTCTTTACTCCTGTTGTATACCTTTGTCAAATTATGAAATTGAACCCCAGTTTTTAAGCTTTAGTTCTTGCATTTTCAACAATTTTTGTTGCATATTATGAATTTTAGATCATGGTTGTATTTATTCCATCAGAATGTAGGGGCATATGTAGGCGTCACTCTTGAATTTATTTTGAATGACTAAGATTATTTTGTGATGTGATTTTAAAATGGCTCTCAAACATGTCTGGCTTTGCTACCTCATATGAATTCAGTTAAAATTCACTCAATGAACTAAAACTAAGAAAAATACAGTTTAAAATTTTAAGATTAAATAAATGCCATGCTTCCTTCCTCTCTACTTTCTTATGTCTCGAACCCCTTTTCCCTAGAGATTCTGTTTTCTTATGTTTTCATCAAGTTTAAAGAAATGTGGAAAAATAGACTGCTTTAATTTATAGACAAGAAAAGAAAGGTTGCTAATCGATAAACAGATGAAGTGTCGGAGATAAAGGATGTTTTAGGAAAAGAAAGCTTCAGTTGGTTCCCATGTAAGTCTAGGAAAAGAAATCTTAGTAACAAGACAAGCTTCGTCCATATGACCTGTATGAAGATCGCCAACCTCACTATGTCCCTAAAATCAAACAAGTCAGTTGCATTTTACCATACTACGTAAACATTTCTTCCAGATCAAGGTAGGAAATTTTCAAGCCTCCCCTGTTTTTCATTCCCTATCAAGTTTGCTTGCCTTCTCTTCCTTCTTTCTGTACCAATCTCTGCATTGTATGCGTATCAATGCATAAGCGAAGGGAAAAAGATTTCACTGCTTGGTTGATGGAGAGAACAGAAACCGGGAAGATTTTGTATTCTAAAGATGCTTTGCTAAAATATTCCGGACTATTATGTGAGTAAAGGTTGTCTGCTGACCAAGAAAATGTCTCAAACTCGTTAATGATGTGAGAGATTTTCTCTTGGCCCCATTAGCAACTTCTTTGTATTGTTGAAGGTTGAGGAAATGAGGTTCAAAGCTTAAAGGTAAACTAACTATTTAATCGTAAGGTTTGAATAGATGTATTATCAATTCCTATTGTTCAAGATAGAGGAAGGCTACTTGATTGGCCTTGGTTATCACATGATTTTAACCCGAAGTGCTTTGTTGGCTCACTAATTTCTAAATGTCAATAGTTTATCCTATTGTTTGGGCCATCTAGTTAAGTCGTCTTTGCAATGATTTAATTGCAGTGCTGTTTGTTTTGGTGAACCGGTGGCTGAGTTATGATTCCGGTTCAGAGGGGATGTTTGAGCATTCACAATTGTTTGGAGGAACATGGCCGTGGGCTTTTGCAGCTTTGTGCTTCTCATGTGGATATTTTGCATATGATCAGTGGGACATGCTGCGATATGGACTATATAGTGGCTGGATTCCTGCGATCTTGGTTCATCATCTGGTTCTTCTCATTTGCTTCACGCTTGCTTTGTATAGGAATGTCACCATCAATTATCTCAATCTCACTCTCATTTGCGAGGTATGCTTTAGTTTATTCTTGTAAAATGTGCCTTGTGAATATGCTGCTTTCTTTCTTCATGTTTGTGGTTCTTTCTGGATTTAAGCATTTCTGTTACGATTACAATGTCTCCTTTGATGACTTCGACACAGGCACAAAGATGAGGTATCCAACTATTTAACGATGGTTTCAGATTTAAGTTGTTTTCAAAGGAACGTAATAGATAGATATTTTATCTAGTGCTTTTGGCTTGAACAGTCCATCTTAATCTCTTGTGGCACCCAGTTTCCTTTTTTGATCACTTTCTCAATGCACTTTCTCTCACTGAGTTCTCTTTGTATTCACCCTTTCACACTGTTCTACTCCCTTACACAATTTCTGACGCTTTACTCTTAGTTACCCATTCCCTTACCTTATTTTTCGTTGCATATTTTCTCTCCATGTATACTTTCTGTCTGGTACATTTGTGTATTTCAAACAATATCAAGTCAAAAACTTACTAAACACTTTTTAAAGTTCATGAACTAATTAGACACAAACCTCAAGAATCATTGACTAAACTTATAATTTAACCTAGTCTATTTCTACCCAATCTTTCTTACTATGTCTCTCCACTTGTCTTCTTTTTTTTACTTTTTGATCTCGTGATGTGCACACATACGGAACATGCCTTTTAGGTGGGAAATTAAACACCTTAGTTAGCAAATGATAGAGAGTGAACTTCTATGCCCGTATTGAAGGACCTTGTTAAATCATCGATTGACCCATATATTAGCTTAAACTTTTAGGTCAATCAATAATTTAATATGGTATTAGGGCAGATGGTCTAGAAGGTTTATGGTCACACTCTTGCAAACCAGATCTATTCTTAGAACAACCATACTCTATTAGAATCAAACCAAGTTTTCAGGTCACCTTTTACTTCCAGCTTTTGATATGCAGAACTAAAACTAGAATCGTTCTGATGTTCCAGATGCATTCTATCTTTCTCCATGTAAGGCGGTTGCGAAGGATGGCTGGTGTTCGTGATGCCAAGAGCATTATCGTGAAAGTTGAATGGATTCTCAACTGGCTAACATTTGTTTTCGCAAGGTTTGTTCCACACATTCTCATTACTGTCAAGCTCATCATAGATGCTTCCAAGTTTGATAAAGGCGTAGAGCTGCCACTTGCATTGTCTGGGATGGCTGGCATGAACCTGCTGAATGCTTTGCTTGGTATTGATCTTTTAAACGCTGTAAAGAAAGAGAGTAATTACAAGAAAACTGATGACACTCATCATGAATGAACGAGGACTAACTTTGGTGGAAATTAACACGGAAGAAAAAATTTCATAATATCAGAGTTTAATATTTAGTTGAAGAAACTTTGTACTATTAATATTGTTTTTAATATAGTTCTTAGGCTTTTAATAAGTTTAGGC

mRNA sequence

GGTTGCCCCTCTCCTCTCTCCGCTCTCTTCATTTCTGTTTATGTCAATCGTCCTCTTCTCCCGCTGATACGGAACACACGATTTAGCTCCACACCGACTCCATGGCGGGTAGAACATTGTTGAAGGAGAAAAGCAGGGCATGGAGCTTCTTCTCCGCGACATTACTGCTTTGGTCAGTCTCTCTTTGGTTCGAAATTCTATTCGACCGACGATTCCGGCTGCTCTACGTCGTTTTCGGAGCTCTGTTCTACCAGTCCGCCAATTCCTCCATTAGATTACTTCTTTCCAAGGATCCTCTTTTAGTCAACACCTCCGTTTCTCTTCTTCATTCCATTATCACCTCTGCTTCAGTGCTGTTTGTTTTGGTGAACCGGTGGCTGAGTTATGATTCCGGTTCAGAGGGGATGTTTGAGCATTCACAATTGTTTGGAGGAACATGGCCGTGGGCTTTTGCAGCTTTGTGCTTCTCATGTGGATATTTTGCATATGATCAGTGGGACATGCTGCGATATGGACTATATAGTGGCTGGATTCCTGCGATCTTGGTTCATCATCTGGTTCTTCTCATTTGCTTCACGCTTGCTTTGTATAGGAATGTCACCATCAATTATCTCAATCTCACTCTCATTTGCGAGATGCATTCTATCTTTCTCCATGTAAGGCGGTTGCGAAGGATGGCTGGTGTTCGTGATGCCAAGAGCATTATCGTGAAAGTTGAATGGATTCTCAACTGGCTAACATTTGTTTTCGCAAGGTTTGTTCCACACATTCTCATTACTGTCAAGCTCATCATAGATGCTTCCAAGTTTGATAAAGGCGTAGAGCTGCCACTTGCATTGTCTGGGATGGCTGGCATGAACCTGCTGAATGCTTTGCTTGGTATTGATCTTTTAAACGCTGTAAAGAAAGAGAGTAATTACAAGAAAACTGATGACACTCATCATGAATGAACGAGGACTAACTTTGGTGGAAATTAACACGGAAGAAAAAATTTCATAATATCAGAGTTTAATATTTAGTTGAAGAAACTTTGTACTATTAATATTGTTTTTAATATAGTTCTTAGGCTTTTAATAAGTTTAGGC

Coding sequence (CDS)

ATGGCGGGTAGAACATTGTTGAAGGAGAAAAGCAGGGCATGGAGCTTCTTCTCCGCGACATTACTGCTTTGGTCAGTCTCTCTTTGGTTCGAAATTCTATTCGACCGACGATTCCGGCTGCTCTACGTCGTTTTCGGAGCTCTGTTCTACCAGTCCGCCAATTCCTCCATTAGATTACTTCTTTCCAAGGATCCTCTTTTAGTCAACACCTCCGTTTCTCTTCTTCATTCCATTATCACCTCTGCTTCAGTGCTGTTTGTTTTGGTGAACCGGTGGCTGAGTTATGATTCCGGTTCAGAGGGGATGTTTGAGCATTCACAATTGTTTGGAGGAACATGGCCGTGGGCTTTTGCAGCTTTGTGCTTCTCATGTGGATATTTTGCATATGATCAGTGGGACATGCTGCGATATGGACTATATAGTGGCTGGATTCCTGCGATCTTGGTTCATCATCTGGTTCTTCTCATTTGCTTCACGCTTGCTTTGTATAGGAATGTCACCATCAATTATCTCAATCTCACTCTCATTTGCGAGATGCATTCTATCTTTCTCCATGTAAGGCGGTTGCGAAGGATGGCTGGTGTTCGTGATGCCAAGAGCATTATCGTGAAAGTTGAATGGATTCTCAACTGGCTAACATTTGTTTTCGCAAGGTTTGTTCCACACATTCTCATTACTGTCAAGCTCATCATAGATGCTTCCAAGTTTGATAAAGGCGTAGAGCTGCCACTTGCATTGTCTGGGATGGCTGGCATGAACCTGCTGAATGCTTTGCTTGGTATTGATCTTTTAAACGCTGTAAAGAAAGAGAGTAATTACAAGAAAACTGATGACACTCATCATGAATGA

Protein sequence

MAGRTLLKEKSRAWSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRLLLSKDPLLVNTSVSLLHSIITSASVLFVLVNRWLSYDSGSEGMFEHSQLFGGTWPWAFAALCFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMHSIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGVELPLALSGMAGMNLLNALLGIDLLNAVKKESNYKKTDDTHHE*

Homology

[Close]

BLAST of CSPI06G20400 vs. ExPASy Swiss-Prot
Match: A8WGS4 (TLC domain-containing protein 2 OS=Danio rerio OX=7955 GN=tlcd2 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 3.4e-06
Identity = 55/200 (27.50%), Postives = 99/200 (49.50%), Query Frame = 0


Query: 69  NTSVSLLHSIITSA-SVLFVLVNRWLSYDSGSEGMFEHSQLFGGTWPWAFAALCFSCGYF 128
           N S S +HS+IT   SVL   ++  +     +E + E   +F      + A +  S GYF
Sbjct: 42  NISTSFVHSLITGVWSVLCFCMHPQM-----AEDLIETHSVF------SHALVSVSIGYF 101

Query: 129 AYDQWDM-LRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMHSIFLHV 188
            YD  DM +   +   W   +L HH+V++ CF +++     + +  + L+ E++S+FLH+
Sbjct: 102 IYDFLDMVINQKIIHSW--ELLFHHVVVITCFGISVLTCRYVGFAVVALLVEINSVFLHL 161

Query: 189 RRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGVELPLAL 248
           R++ RMA +  AKS   +V  ++N  T+V  R      +T  L+++           +  
Sbjct: 162 RQVLRMANL--AKSTFYRVNSMINLGTYVVFRINTLAWMTRWLVLNRDLIPL-FSYTIGS 221

Query: 249 SGMAGMNLLNALLGIDLLNA 267
            G+A M  +N +L   L+ +
Sbjct: 222 VGLAIMTAMNIVLFYRLMRS 225

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BLAST of CSPI06G20400 vs. ExPASy Swiss-Prot
Match: Q8VC26 (TLC domain-containing protein 2 OS=Mus musculus OX=10090 GN=Tlcd2 PE=2 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 3.8e-05
Identity = 53/198 (26.77%), Postives = 93/198 (46.97%), Query Frame = 0


Query: 69  NTSVSLLHSIITSASVLFVLVNRWLSYDSGSEGMFEHSQLFGGTWPWAFAALCFSCGYFA 128
           N  VSL+HS+++       LV  W      ++ + +H        PWA   +  S GYFA
Sbjct: 40  NIFVSLIHSLLSGVG---ALVGLWQFPQMVTDPINDHP-------PWARVLVAVSVGYFA 99

Query: 129 YDQWDML-RYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMHSIFLHVR 188
            D  DML    L   W   +L HHL ++ C + A+     + +  ++L+ E++SI LH+R
Sbjct: 100 ADGVDMLWNQTLAQAW--DLLCHHLAVVSCLSTAVVSGHYVGFSMVSLLLELNSICLHLR 159

Query: 189 RLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGVELP-LAL 248
           +L  ++    A S+  +V    +  T V  R +P  L  + L +    +   + L  L +
Sbjct: 160 KLLLLS--HKAPSLAFRVSSWASLATLVLFRLLP--LGWMSLWLSRQHYQLSLALVLLCV 219

Query: 249 SGMAGMNLLNALLGIDLL 265
           +G+  +  ++   GI +L
Sbjct: 220 AGLVTVGSISISTGIRIL 221

[Close]

BLAST of CSPI06G20400 vs. ExPASy TrEMBL
Match: A0A0A0KH54 (TLC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G385110 PE=4 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 5.6e-153
Identity = 281/282 (99.65%), Postives = 281/282 (99.65%), Query Frame = 0


Query: 1   MAGRTLLKEKSRAWSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRLL 60
           MAGRTLLKEKSRAWSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRLL
Sbjct: 1   MAGRTLLKEKSRAWSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRLL 60

Query: 61  LSKDPLLVNTSVSLLHSIITSASVLFVLVNRWLSYDSGSEGMFEHSQLFGGTWPWAFAAL 120
           LSKDPLLVNTSVSLLHSIITSASVLFVLVNRWLSY SGSEGMFEHSQLFGGTWPWAFAAL
Sbjct: 61  LSKDPLLVNTSVSLLHSIITSASVLFVLVNRWLSYGSGSEGMFEHSQLFGGTWPWAFAAL 120

Query: 121 CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMH 180
           CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMH
Sbjct: 121 CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMH 180

Query: 181 SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGV 240
           SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGV
Sbjct: 181 SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGV 240

Query: 241 ELPLALSGMAGMNLLNALLGIDLLNAVKKESNYKKTDDTHHE 283
           ELPLALSGMAGMNLLNALLGIDLLNAVKKESNYKKTDDTHHE
Sbjct: 241 ELPLALSGMAGMNLLNALLGIDLLNAVKKESNYKKTDDTHHE 282

[Close]

BLAST of CSPI06G20400 vs. ExPASy TrEMBL
Match: A0A1S3CRX5 (TLC domain-containing protein 2 OS=Cucumis melo OX=3656 GN=LOC103504058 PE=4 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 9.2e-148
Identity = 271/282 (96.10%), Postives = 277/282 (98.23%), Query Frame = 0


Query: 1   MAGRTLLKEKSRAWSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRLL 60
           MAGRTLLKEKSRA SFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIR+L
Sbjct: 1   MAGRTLLKEKSRAGSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRVL 60

Query: 61  LSKDPLLVNTSVSLLHSIITSASVLFVLVNRWLSYDSGSEGMFEHSQLFGGTWPWAFAAL 120
           +SKDPLLVNTSVSLLHSI+TSASVLFVLVNRWLSYDS SEGMFEHSQLFGGTWPWA+AAL
Sbjct: 61  VSKDPLLVNTSVSLLHSIVTSASVLFVLVNRWLSYDSSSEGMFEHSQLFGGTWPWAYAAL 120

Query: 121 CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMH 180
           CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYL LTL+CEMH
Sbjct: 121 CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLILTLVCEMH 180

Query: 181 SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGV 240
           SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDA KFDKGV
Sbjct: 181 SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDAYKFDKGV 240

Query: 241 ELPLALSGMAGMNLLNALLGIDLLNAVKKESNYKKTDDTHHE 283
           ELPLALSGM GMNLLNALLGIDLLNAV+KESNYKKTDDTHHE
Sbjct: 241 ELPLALSGMTGMNLLNALLGIDLLNAVRKESNYKKTDDTHHE 282

[Close]

BLAST of CSPI06G20400 vs. ExPASy TrEMBL
Match: A0A6J1FID3 (TLC domain-containing protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111445599 PE=4 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 1.1e-132
Identity = 245/282 (86.88%), Postives = 262/282 (92.91%), Query Frame = 0


Query: 1   MAGRTLLKEKSRAWSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRLL 60
           MAGRT+LKEKSRA SFFSATL+LW+VSLWFEILFDRRF LLY+VFGALFYQSANS+IR L
Sbjct: 1   MAGRTVLKEKSRAGSFFSATLVLWTVSLWFEILFDRRFELLYLVFGALFYQSANSAIRFL 60

Query: 61  LSKDPLLVNTSVSLLHSIITSASVLFVLVNRWLSYDSGSEGMFEHSQLFGGTWPWAFAAL 120
           +SK+PL+VNT+VSLLHSIITSASVLFVL NRWLS+  GS+GMFEHSQLFGGTWPWA+AAL
Sbjct: 61  ISKEPLVVNTAVSLLHSIITSASVLFVLANRWLSH--GSDGMFEHSQLFGGTWPWAYAAL 120

Query: 121 CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMH 180
           CFSCGYFAYDQWDML+Y LYSGWIPAILVHHLVLLICFTLALYRNVTINYL LTLICE+H
Sbjct: 121 CFSCGYFAYDQWDMLQYRLYSGWIPAILVHHLVLLICFTLALYRNVTINYLILTLICELH 180

Query: 181 SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGV 240
           SIFLHVRRLRRMAGVRDA+S IVKVEW LNWLTF FARFVPHILIT KLI+DASKFDKGV
Sbjct: 181 SIFLHVRRLRRMAGVRDAESFIVKVEWTLNWLTFTFARFVPHILITAKLIMDASKFDKGV 240

Query: 241 ELPLALSGMAGMNLLNALLGIDLLNAVKKESNYKKTDDTHHE 283
           ELPLAL GMA MNLLN  LGIDL NAVKKESNY+K D  HHE
Sbjct: 241 ELPLALFGMAAMNLLNVFLGIDLFNAVKKESNYQKNDHLHHE 280

[Close]

BLAST of CSPI06G20400 vs. ExPASy TrEMBL
Match: A0A6J1K0R9 (TLC domain-containing protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111489133 PE=4 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 4.2e-132
Identity = 243/282 (86.17%), Postives = 263/282 (93.26%), Query Frame = 0


Query: 1   MAGRTLLKEKSRAWSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRLL 60
           MAGRT+LKE+SRA SFFSATL+LW+VSLWFEILFDRRF LLY+VFGALFYQSANS+IR L
Sbjct: 1   MAGRTVLKEESRAGSFFSATLVLWTVSLWFEILFDRRFELLYLVFGALFYQSANSAIRFL 60

Query: 61  LSKDPLLVNTSVSLLHSIITSASVLFVLVNRWLSYDSGSEGMFEHSQLFGGTWPWAFAAL 120
           +SK+PL+VNT+VSLLHSIITSASVLF+LVNRWLS+  G +GMFEHSQLFGGTWPWA+AAL
Sbjct: 61  ISKEPLVVNTAVSLLHSIITSASVLFILVNRWLSH--GLDGMFEHSQLFGGTWPWAYAAL 120

Query: 121 CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMH 180
           CFSCGYFAYDQWDML+Y LYSGWIPAILVHHLVLLICFTLALYRNVTINYL LTLICE+H
Sbjct: 121 CFSCGYFAYDQWDMLQYRLYSGWIPAILVHHLVLLICFTLALYRNVTINYLILTLICELH 180

Query: 181 SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGV 240
           SIFLHVRRLRRMAGVRDA+S IVKVEW LNWLTF+FARFVPHILIT KLI+DASKFDKGV
Sbjct: 181 SIFLHVRRLRRMAGVRDAESFIVKVEWTLNWLTFIFARFVPHILITAKLIMDASKFDKGV 240

Query: 241 ELPLALSGMAGMNLLNALLGIDLLNAVKKESNYKKTDDTHHE 283
           ELPLAL GMA MNLLN  LGIDL NAVKKESNY+K D  HHE
Sbjct: 241 ELPLALFGMAAMNLLNVFLGIDLFNAVKKESNYQKNDHFHHE 280

[Close]

BLAST of CSPI06G20400 vs. ExPASy TrEMBL
Match: A0A6J1CKF5 (TLC domain-containing protein 2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011955 PE=4 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.2e-124
Identity = 236/282 (83.69%), Postives = 253/282 (89.72%), Query Frame = 0


Query: 1   MAGRTLLKEKSRAWSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRLL 60
           MAGR + KEKSRA SFFSATL+LW VS+WFEILF RR  LLYVVFGALFYQSAN ++R L
Sbjct: 1   MAGRRVPKEKSRAGSFFSATLVLWIVSIWFEILFYRRSELLYVVFGALFYQSANWTVRAL 60

Query: 61  LSKDPLLVNTSVSLLHSIITSASVLFVLVNRWLSYDSGSEGMFEHSQLFGGTWPWAFAAL 120
           +SKDPL VNTSVSLLHS ITSASVLF+LV++WLSY  GS GMFEHSQL GGTW  A+AAL
Sbjct: 61  VSKDPLFVNTSVSLLHSTITSASVLFILVSQWLSY--GSHGMFEHSQLVGGTWSGAYAAL 120

Query: 121 CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMH 180
           CFSCGYFAYDQWDML Y LYSGWIPAILVHHLVLLICFTLALYRNVTINYL LTLICE+H
Sbjct: 121 CFSCGYFAYDQWDMLLYQLYSGWIPAILVHHLVLLICFTLALYRNVTINYLILTLICELH 180

Query: 181 SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGV 240
           SIFLHVRRLRRMAGVRDAKSI+VKVEWILNWLTFVFAR VPHILIT KL++DASKFD+GV
Sbjct: 181 SIFLHVRRLRRMAGVRDAKSIVVKVEWILNWLTFVFARVVPHILITAKLVVDASKFDRGV 240

Query: 241 ELPLALSGMAGMNLLNALLGIDLLNAVKKESNYKKTDDTHHE 283
           ELPLALSGMAGMNLLNA LGIDL+NAVKKES Y+K D    E
Sbjct: 241 ELPLALSGMAGMNLLNAFLGIDLINAVKKESKYQKRDHPRRE 280

[Close]

BLAST of CSPI06G20400 vs. NCBI nr
Match: XP_004150440.1 (TLC domain-containing protein 2 [Cucumis sativus] >KGN47727.1 hypothetical protein Csa_018230 [Cucumis sativus])

HSP 1 Score: 550.1 bits (1416), Expect = 1.2e-152
Identity = 281/282 (99.65%), Postives = 281/282 (99.65%), Query Frame = 0


Query: 1   MAGRTLLKEKSRAWSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRLL 60
           MAGRTLLKEKSRAWSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRLL
Sbjct: 1   MAGRTLLKEKSRAWSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRLL 60

Query: 61  LSKDPLLVNTSVSLLHSIITSASVLFVLVNRWLSYDSGSEGMFEHSQLFGGTWPWAFAAL 120
           LSKDPLLVNTSVSLLHSIITSASVLFVLVNRWLSY SGSEGMFEHSQLFGGTWPWAFAAL
Sbjct: 61  LSKDPLLVNTSVSLLHSIITSASVLFVLVNRWLSYGSGSEGMFEHSQLFGGTWPWAFAAL 120

Query: 121 CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMH 180
           CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMH
Sbjct: 121 CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMH 180

Query: 181 SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGV 240
           SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGV
Sbjct: 181 SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGV 240

Query: 241 ELPLALSGMAGMNLLNALLGIDLLNAVKKESNYKKTDDTHHE 283
           ELPLALSGMAGMNLLNALLGIDLLNAVKKESNYKKTDDTHHE
Sbjct: 241 ELPLALSGMAGMNLLNALLGIDLLNAVKKESNYKKTDDTHHE 282

[Close]

BLAST of CSPI06G20400 vs. NCBI nr
Match: XP_008466713.1 (PREDICTED: TLC domain-containing protein 2 [Cucumis melo])

HSP 1 Score: 532.7 bits (1371), Expect = 1.9e-147
Identity = 271/282 (96.10%), Postives = 277/282 (98.23%), Query Frame = 0


Query: 1   MAGRTLLKEKSRAWSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRLL 60
           MAGRTLLKEKSRA SFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIR+L
Sbjct: 1   MAGRTLLKEKSRAGSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRVL 60

Query: 61  LSKDPLLVNTSVSLLHSIITSASVLFVLVNRWLSYDSGSEGMFEHSQLFGGTWPWAFAAL 120
           +SKDPLLVNTSVSLLHSI+TSASVLFVLVNRWLSYDS SEGMFEHSQLFGGTWPWA+AAL
Sbjct: 61  VSKDPLLVNTSVSLLHSIVTSASVLFVLVNRWLSYDSSSEGMFEHSQLFGGTWPWAYAAL 120

Query: 121 CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMH 180
           CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYL LTL+CEMH
Sbjct: 121 CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLILTLVCEMH 180

Query: 181 SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGV 240
           SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDA KFDKGV
Sbjct: 181 SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDAYKFDKGV 240

Query: 241 ELPLALSGMAGMNLLNALLGIDLLNAVKKESNYKKTDDTHHE 283
           ELPLALSGM GMNLLNALLGIDLLNAV+KESNYKKTDDTHHE
Sbjct: 241 ELPLALSGMTGMNLLNALLGIDLLNAVRKESNYKKTDDTHHE 282

[Close]

BLAST of CSPI06G20400 vs. NCBI nr
Match: XP_038884583.1 (TLC domain-containing protein 2 [Benincasa hispida])

HSP 1 Score: 497.3 bits (1279), Expect = 8.9e-137
Identity = 255/282 (90.43%), Postives = 270/282 (95.74%), Query Frame = 0


Query: 1   MAGRTLLKEKSRAWSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRLL 60
           MAGRT+LKEK RA SFFSATL+LWSVSL FEILFDRRF LLYVVFGALFYQSANS+IR L
Sbjct: 1   MAGRTVLKEKRRAGSFFSATLVLWSVSLCFEILFDRRFELLYVVFGALFYQSANSAIRFL 60

Query: 61  LSKDPLLVNTSVSLLHSIITSASVLFVLVNRWLSYDSGSEGMFEHSQLFGGTWPWAFAAL 120
           +SKDPLLVNTSVSLLHS ITSASVLFVLVNRWLSY  GSEG+FEHSQLFGGTWPWA+AAL
Sbjct: 61  VSKDPLLVNTSVSLLHSTITSASVLFVLVNRWLSY--GSEGIFEHSQLFGGTWPWAYAAL 120

Query: 121 CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMH 180
           CFSCGYFAYDQWDML+YGLYSGWIPAILVHHLVLLICFTLALYRNVTINYL LTLICEMH
Sbjct: 121 CFSCGYFAYDQWDMLQYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLILTLICEMH 180

Query: 181 SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGV 240
           SIFLHVRRLRRMAGVRDAKS+IVKVEWILNWLTFVFARFVPHILIT KLI+DASKFDKG+
Sbjct: 181 SIFLHVRRLRRMAGVRDAKSVIVKVEWILNWLTFVFARFVPHILITAKLIMDASKFDKGI 240

Query: 241 ELPLALSGMAGMNLLNALLGIDLLNAVKKESNYKKTDDTHHE 283
           ELPLALSGMAGMNLLNALLG+DLL+AV+KES Y+KTD +HHE
Sbjct: 241 ELPLALSGMAGMNLLNALLGMDLLSAVRKESYYQKTDHSHHE 280

[Close]

BLAST of CSPI06G20400 vs. NCBI nr
Match: XP_023550889.1 (TLC domain-containing protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 483.0 bits (1242), Expect = 1.7e-132
Identity = 245/282 (86.88%), Postives = 264/282 (93.62%), Query Frame = 0


Query: 1   MAGRTLLKEKSRAWSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRLL 60
           MAGRT+LKEKSRA SFFSATL+LW+VSLWFEILFDRRF LLY+VFGALFYQSANS+IR L
Sbjct: 1   MAGRTMLKEKSRAGSFFSATLVLWTVSLWFEILFDRRFELLYLVFGALFYQSANSAIRFL 60

Query: 61  LSKDPLLVNTSVSLLHSIITSASVLFVLVNRWLSYDSGSEGMFEHSQLFGGTWPWAFAAL 120
           +SK+PL+VNT+VSLLHSIITSASVLFVLVNRWLS+  GS+GMFEHSQLFGGTWPWA+AAL
Sbjct: 61  ISKEPLVVNTAVSLLHSIITSASVLFVLVNRWLSH--GSDGMFEHSQLFGGTWPWAYAAL 120

Query: 121 CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMH 180
           CFSCGYFAYDQWDML+Y LYSGWIPAILVHHLVLLICFTLALYRNVTINYL LTLICE+H
Sbjct: 121 CFSCGYFAYDQWDMLQYRLYSGWIPAILVHHLVLLICFTLALYRNVTINYLILTLICELH 180

Query: 181 SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGV 240
           SIFLHVRRLRRMAGVRDA+S IVKVEW LNWLTF+FARFVPHILIT KLI+DASKFDKGV
Sbjct: 181 SIFLHVRRLRRMAGVRDAESFIVKVEWTLNWLTFIFARFVPHILITAKLIMDASKFDKGV 240

Query: 241 ELPLALSGMAGMNLLNALLGIDLLNAVKKESNYKKTDDTHHE 283
           ELPLAL GMA MNLLN  LGIDL NAVKKESNY+K D  H+E
Sbjct: 241 ELPLALFGMAAMNLLNVFLGIDLFNAVKKESNYQKNDHLHNE 280

[Close]

BLAST of CSPI06G20400 vs. NCBI nr
Match: XP_022939854.1 (TLC domain-containing protein 2 [Cucurbita moschata] >KAG6578966.1 TLC domain-containing protein 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016488.1 TLC domain-containing protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 482.6 bits (1241), Expect = 2.3e-132
Identity = 245/282 (86.88%), Postives = 262/282 (92.91%), Query Frame = 0


Query: 1   MAGRTLLKEKSRAWSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRLL 60
           MAGRT+LKEKSRA SFFSATL+LW+VSLWFEILFDRRF LLY+VFGALFYQSANS+IR L
Sbjct: 1   MAGRTVLKEKSRAGSFFSATLVLWTVSLWFEILFDRRFELLYLVFGALFYQSANSAIRFL 60

Query: 61  LSKDPLLVNTSVSLLHSIITSASVLFVLVNRWLSYDSGSEGMFEHSQLFGGTWPWAFAAL 120
           +SK+PL+VNT+VSLLHSIITSASVLFVL NRWLS+  GS+GMFEHSQLFGGTWPWA+AAL
Sbjct: 61  ISKEPLVVNTAVSLLHSIITSASVLFVLANRWLSH--GSDGMFEHSQLFGGTWPWAYAAL 120

Query: 121 CFSCGYFAYDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMH 180
           CFSCGYFAYDQWDML+Y LYSGWIPAILVHHLVLLICFTLALYRNVTINYL LTLICE+H
Sbjct: 121 CFSCGYFAYDQWDMLQYRLYSGWIPAILVHHLVLLICFTLALYRNVTINYLILTLICELH 180

Query: 181 SIFLHVRRLRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGV 240
           SIFLHVRRLRRMAGVRDA+S IVKVEW LNWLTF FARFVPHILIT KLI+DASKFDKGV
Sbjct: 181 SIFLHVRRLRRMAGVRDAESFIVKVEWTLNWLTFTFARFVPHILITAKLIMDASKFDKGV 240

Query: 241 ELPLALSGMAGMNLLNALLGIDLLNAVKKESNYKKTDDTHHE 283
           ELPLAL GMA MNLLN  LGIDL NAVKKESNY+K D  HHE
Sbjct: 241 ELPLALFGMAAMNLLNVFLGIDLFNAVKKESNYQKNDHLHHE 280

[Close]

BLAST of CSPI06G20400 vs. TAIR 10
Match: AT1G21790.1 (TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein )

HSP 1 Score: 357.8 bits (917), Expect = 7.8e-99
Identity = 181/270 (67.04%), Postives = 223/270 (82.59%), Query Frame = 0


Query: 9   EKSRAWSFFSATLLLWSVSLWFEILFDRRFRLLYVVFGALFYQSANSSIRLLLSKDPLLV 68
           +K+ A  FF ATLLLW VS+ FEI F+ R  LL+V+ G  F+Q  N  +R  +S+DP+ V
Sbjct: 12  KKNGAGQFFLATLLLWLVSVVFEIAFNLRTELLWVICGGCFFQLMNWVVRSWISRDPIFV 71

Query: 69  NTSVSLLHSIITSASVLFVLVNRWLSYDSGSEGMFEHSQLFGGTWPWAFAALCFSCGYFA 128
           NTSVSLLHSIITSASV+F+L+N+ L+   G + MF+HS+L GGTW WA+ ALCFSCGYFA
Sbjct: 72  NTSVSLLHSIITSASVVFILLNQCLA--KGLDEMFDHSELVGGTWKWAYPALCFSCGYFA 131

Query: 129 YDQWDMLRYGLYSGWIPAILVHHLVLLICFTLALYRNVTINYLNLTLICEMHSIFLHVRR 188
           YDQWDML+Y LYSG IP+ILVHHLVLL+CFTLALYRNVTINYL LTLICEMHSIFLHVR+
Sbjct: 132 YDQWDMLQYRLYSGLIPSILVHHLVLLVCFTLALYRNVTINYLILTLICEMHSIFLHVRK 191

Query: 189 LRRMAGVRDAKSIIVKVEWILNWLTFVFARFVPHILITVKLIIDASKFDKGVELPLALSG 248
           LRRMAG+RD+ + +VK+EW+LNW  FVFAR +PHILIT+KLI DA KF KGVELPLALSG
Sbjct: 192 LRRMAGIRDSNTALVKLEWVLNWTAFVFARCIPHILITIKLIKDAHKFGKGVELPLALSG 251

Query: 249 MAGMNLLNALLGIDLLNAVKKESNYKKTDD 279
           MAGMN+LN  LG+DL +A ++E ++++  +
Sbjct: 252 MAGMNILNVGLGLDLFHAFRRERSHRRNQE 279

The following BLAST results are available for this feature:

BLAST of CSPI06G20400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A8WGS4	3.4e-06	27.50	TLC domain-containing protein 2 OS=Danio rerio OX=7955 GN=tlcd2 PE=2 SV=1	[more]
Q8VC26	3.8e-05	26.77	TLC domain-containing protein 2 OS=Mus musculus OX=10090 GN=Tlcd2 PE=2 SV=1	[more]

BLAST of CSPI06G20400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KH54	5.6e-153	99.65	TLC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G385110 PE=4 SV...	[more]
A0A1S3CRX5	9.2e-148	96.10	TLC domain-containing protein 2 OS=Cucumis melo OX=3656 GN=LOC103504058 PE=4 SV=...	[more]
A0A6J1FID3	1.1e-132	86.88	TLC domain-containing protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111445599 PE...	[more]
A0A6J1K0R9	4.2e-132	86.17	TLC domain-containing protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111489133 PE=4...	[more]
A0A6J1CKF5	3.2e-124	83.69	TLC domain-containing protein 2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC...	[more]

BLAST of CSPI06G20400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004150440.1	1.2e-152	99.65	TLC domain-containing protein 2 [Cucumis sativus] >KGN47727.1 hypothetical prote...	[more]
XP_008466713.1	1.9e-147	96.10	PREDICTED: TLC domain-containing protein 2 [Cucumis melo]	[more]
XP_038884583.1	8.9e-137	90.43	TLC domain-containing protein 2 [Benincasa hispida]	[more]
XP_023550889.1	1.7e-132	86.88	TLC domain-containing protein 2 [Cucurbita pepo subsp. pepo]	[more]
XP_022939854.1	2.3e-132	86.88	TLC domain-containing protein 2 [Cucurbita moschata] >KAG6578966.1 TLC domain-co...	[more]

BLAST of CSPI06G20400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G21790.1	7.8e-99	67.04	TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006634	TRAM/LAG1/CLN8 homology domain	SMART	SM00724	lag1_27	coord: 62..269 e-value: 1.0E-25 score: 101.4
IPR006634	TRAM/LAG1/CLN8 homology domain	PFAM	PF03798	TRAM_LAG1_CLN8	coord: 69..257 e-value: 9.9E-18 score: 64.6
IPR006634	TRAM/LAG1/CLN8 homology domain	PROSITE	PS50922	TLC	coord: 62..281 score: 12.947504
None	No IPR available	PANTHER	PTHR13439	CT120 PROTEIN	coord: 16..277
None	No IPR available	PANTHER	PTHR13439:SF4	TLC DOMAIN-CONTAINING PROTEIN FLD-1	coord: 16..277

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G20400.1	CSPI06G20400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071709	membrane assembly
biological_process	GO:0055091	phospholipid homeostasis
biological_process	GO:0007009	plasma membrane organization
biological_process	GO:0097035	regulation of membrane lipid distribution
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane