CSPI06G18550 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G18550
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationChr6: 16864666 .. 16866223 (-)
RNA-Seq ExpressionCSPI06G18550
SyntenyCSPI06G18550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTCTCTCTCTCTCTCTCTCTCTCTTCTTTTGGTTGTTTGGAAGCTCCTGTTGCTTCCCCTAATCACATCCCTTCCTTTCTACCTCGTATTCCCCCCATCGCATCTCACTCCGACCTCAGAAAAAGTCCCAATTCCATCTCCACCATCATCAATTTCACCCCTATCCTTCTCCCTTCTTTCATGGGTGACAGCCAACTACCCTCTAGTGAAGTCCACTTTGGTCAACCAGCCCTCCTGGATTGTTTTTTTCAGGAAATTAGTCTTGCCCTGATTCTTACCTGCTGAAAGGAATCACCAAGCTTTTGGATTTTTTTAATGTGCTTGCTTTTAGCTTCCCCTGGCACGCGTGATCACGATTGGGTCGTAGCCATTGCAGGTTTGATTTCGGGGTTATGCTGTTTTTGATTTGTTCAATTCTGAAATGTTATTTTGACGTTGTTGTGATTGTTGGGATTGGTTTTTATTTTTTTATTTTTCTTTTGAAGTTCATTCGGGTTTCTTGTGCTACTGTGTGGTTAATCTTGTGCTGGCTCTGTTTCTCAGGGCTTGATTTATAGATCGGATGGCAGAGAGTTGGGATGGAAGTTATGAGGATTCTGGAAGTATATCTGACGAGAGTAGTTATTATGCGAGACTGCATATTGAACCTATTTATGATTCGTTTTTGTGCCCTCTAACGAAGCAAGTAATGAGGGATCCTGTTACTATAGAAAGTGGGCAAACGTTTGAACGTGCGGCCATTGAAATGTGGTTCAACGAATGTAAGGAAAGTAGAAGGAGGCCAATTTGTCCAATGACACTTAAGGAATTAAGAAGCACGGAATTGAATCCCAGTATTGCTTTGCGGAATACTATTGAAGAGTGGACTGCTAGAAATGAAGCTGTTCAGCTAGATATGGCTCGTAAGTCACTTAACTTGAGTAGTCCAGAAAATGAAACTTTGGGATCTTTGAAGTATGTTCAACATGTCTGCCAAAAGGGTTTGTCAAGGCACATCGCACGAAATGCTGGGTTAATACCTATGATTGTTAGCTTGTTGAAGAGCACAAGTCGAAAAGTCCAGTTTAGAGCTTTGGAAACCCTTAGAATTGTGGCACAAGAAGACAGTGAGTGTAAGGTTGTCATTCACGAGTTATAGTATGATTTCATTGGCATGTCAAACTCGGAGTTTCGTGAACCTCTTGTTTCATTTGGCAGGAAATGTTAGCTGAAGGTGACACTCTTCACACAGTAGTAAAGTTCCTGCGTCATGAGCGTTCAAAGGAGAAGGAGGAAGCTGTAGCTTTGCTGTATGAGCTTTCCAAGTCTGAAGCCTTGTGTGAAGAGATCGGTTCAGTTAATGGGGCAATTCTTATATTAGTTGGAATGTCAAGCAGTAAATCTGAGAACATCTCCACAGTTGAAAATGCTGATAGAACATTAGAAAACCTGGAGGTGTGTGAGAATAACATTCGACAAATGGCTGAATATGGTAGACTGAGGCCTCTTCTGACACAGATTCTTGAAGGTATGTACGTGATCTCCAGTTATAGAGTTCTCTGTAGTTATTAGTAGTT

mRNA sequence

CTCTCTCTCTCTCTCTCTCTCTCTCTTCTTTTGGTTGTTTGGAAGCTCCTGTTGCTTCCCCTAATCACATCCCTTCCTTTCTACCTCGTATTCCCCCCATCGCATCTCACTCCGACCTCAGAAAAAGTCCCAATTCCATCTCCACCATCATCAATTTCACCCCTATCCTTCTCCCTTCTTTCATGGGTGACAGCCAACTACCCTCTAGTGAAGTCCACTTTGGTCAACCAGCCCTCCTGGATTGTTTTTTTCAGGAAATTAGTCTTGCCCTGATTCTTACCTGCTGAAAGGAATCACCAAGCTTTTGGATTTTTTTAATGTGCTTGCTTTTAGCTTCCCCTGGCACGCGTGATCACGATTGGGTCGTAGCCATTGCAGGGCTTGATTTATAGATCGGATGGCAGAGAGTTGGGATGGAAGTTATGAGGATTCTGGAAGTATATCTGACGAGAGTAGTTATTATGCGAGACTGCATATTGAACCTATTTATGATTCGTTTTTGTGCCCTCTAACGAAGCAAGTAATGAGGGATCCTGTTACTATAGAAAGTGGGCAAACGTTTGAACGTGCGGCCATTGAAATGTGGTTCAACGAATGTAAGGAAAGTAGAAGGAGGCCAATTTGTCCAATGACACTTAAGGAATTAAGAAGCACGGAATTGAATCCCAGTATTGCTTTGCGGAATACTATTGAAGAGTGGACTGCTAGAAATGAAGCTGTTCAGCTAGATATGGCTCGTAAGTCACTTAACTTGAGTAGTCCAGAAAATGAAACTTTGGGATCTTTGAAGTATGTTCAACATGTCTGCCAAAAGGGTTTGTCAAGGCACATCGCACGAAATGCTGGGTTAATACCTATGATTGTTAGCTTGTTGAAGAGCACAAGTCGAAAAGTCCAGTTTAGAGCTTTGGAAACCCTTAGAATTGTGGCACAAGAAGACAGTGAGTGTAAGGAAATGTTAGCTGAAGGTGACACTCTTCACACAGTAGTAAAGTTCCTGCGTCATGAGCGTTCAAAGGAGAAGGAGGAAGCTGTAGCTTTGCTGTATGAGCTTTCCAAGTCTGAAGCCTTGTGTGAAGAGATCGGTTCAGTTAATGGGGCAATTCTTATATTAGTTGGAATGTCAAGCAGTAAATCTGAGAACATCTCCACAGTTGAAAATGCTGATAGAACATTAGAAAACCTGGAGGTGTGTGAGAATAACATTCGACAAATGGCTGAATATGGTAGACTGAGGCCTCTTCTGACACAGATTCTTGAAGGTATGTACGTGATCTCCAGTTATAGAGTTCTCTGTAGTTATTAGTAGTT

Coding sequence (CDS)

ATGGCAGAGAGTTGGGATGGAAGTTATGAGGATTCTGGAAGTATATCTGACGAGAGTAGTTATTATGCGAGACTGCATATTGAACCTATTTATGATTCGTTTTTGTGCCCTCTAACGAAGCAAGTAATGAGGGATCCTGTTACTATAGAAAGTGGGCAAACGTTTGAACGTGCGGCCATTGAAATGTGGTTCAACGAATGTAAGGAAAGTAGAAGGAGGCCAATTTGTCCAATGACACTTAAGGAATTAAGAAGCACGGAATTGAATCCCAGTATTGCTTTGCGGAATACTATTGAAGAGTGGACTGCTAGAAATGAAGCTGTTCAGCTAGATATGGCTCGTAAGTCACTTAACTTGAGTAGTCCAGAAAATGAAACTTTGGGATCTTTGAAGTATGTTCAACATGTCTGCCAAAAGGGTTTGTCAAGGCACATCGCACGAAATGCTGGGTTAATACCTATGATTGTTAGCTTGTTGAAGAGCACAAGTCGAAAAGTCCAGTTTAGAGCTTTGGAAACCCTTAGAATTGTGGCACAAGAAGACAGTGAGTGTAAGGAAATGTTAGCTGAAGGTGACACTCTTCACACAGTAGTAAAGTTCCTGCGTCATGAGCGTTCAAAGGAGAAGGAGGAAGCTGTAGCTTTGCTGTATGAGCTTTCCAAGTCTGAAGCCTTGTGTGAAGAGATCGGTTCAGTTAATGGGGCAATTCTTATATTAGTTGGAATGTCAAGCAGTAAATCTGAGAACATCTCCACAGTTGAAAATGCTGATAGAACATTAGAAAACCTGGAGGTGTGTGAGAATAACATTCGACAAATGGCTGAATATGGTAGACTGAGGCCTCTTCTGACACAGATTCTTGAAGGTATGTACGTGATCTCCAGTTATAGAGTTCTCTGTAGTTATTAG

Protein sequence

MAESWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAIEMWFNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLSSPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQEDSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILVGMSSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEGMYVISSYRVLCSY*
Homology
BLAST of CSPI06G18550 vs. ExPASy Swiss-Prot
Match: Q9LM76 (U-box domain-containing protein 44 OS=Arabidopsis thaliana OX=3702 GN=PUB44 PE=1 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 1.4e-85
Identity = 160/278 (57.55%), Postives = 223/278 (80.22%), Query Frame = 0

Query: 13  GSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAIEMWFNECKESRR 72
           G  SD+SS++ R  ++ IY++F+CPLTK+VM DPVT+E+G+TFER AIE WF EC++S R
Sbjct: 7   GDQSDDSSHFER-GVDHIYEAFICPLTKEVMHDPVTLENGRTFEREAIEKWFKECRDSGR 66

Query: 73  RPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLSSPENETLGSLKY 132
            P CP+T +EL ST+++ SIALRNTIEEW +RN+A +LD+AR+SL L + E + L +L +
Sbjct: 67  PPSCPLTSQELTSTDVSASIALRNTIEEWRSRNDAAKLDIARQSLFLGNAETDILQALMH 126

Query: 133 VQHVCQKGLS-RHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQEDSECKEMLAEG 192
           V+ +C+   S RH  RN+ LI MI+ +LKSTS +V+++AL+TL++V + D E K ++AEG
Sbjct: 127 VRQICRTIRSNRHGVRNSQLIHMIIDMLKSTSHRVRYKALQTLQVVVEGDDESKAIVAEG 186

Query: 193 DTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILVGMSSSKSENIS 252
           DT+ T+VKFL HE SK +E AV+LL+ELSKSEALCE+IGS++GA+++LVG++SS SEN+S
Sbjct: 187 DTVRTLVKFLSHEPSKGREAAVSLLFELSKSEALCEKIGSIHGALILLVGLTSSNSENVS 246

Query: 253 TVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 290
            VE ADRTLEN+E  E  +RQMA YGRL+PLL ++LEG
Sbjct: 247 IVEKADRTLENMERSEEIVRQMASYGRLQPLLGKLLEG 283

BLAST of CSPI06G18550 vs. ExPASy Swiss-Prot
Match: Q9SFX2 (U-box domain-containing protein 43 OS=Arabidopsis thaliana OX=3702 GN=PUB43 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.3e-80
Identity = 159/287 (55.40%), Postives = 216/287 (75.26%), Query Frame = 0

Query: 4   SWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAIEMW 63
           SWDGS  D+ S  +         I+ IY++F+CPLTKQVM +PVT+E+GQTFER AIE W
Sbjct: 6   SWDGSQSDNSSQFEPG-------IDNIYEAFICPLTKQVMHNPVTLENGQTFEREAIEKW 65

Query: 64  FNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLSSPE 123
           F EC+E+ +   CP+T KEL  T+L+PSIALRNTIEEW ARN+A++LD+AR+SL L + E
Sbjct: 66  FQECRENGQPLSCPITSKELSITDLSPSIALRNTIEEWRARNDALKLDIARQSLYLGNAE 125

Query: 124 NETLGSLKYVQHVCQK-GLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQEDS 183
              L +LK V+ +C+     R    N  L+ +I  +LKS+S +V+ +AL+TL++V + D 
Sbjct: 126 TNILLALKNVREICRNIRKIRQRVCNPQLVRLITDMLKSSSHEVRCKALQTLQVVVEGDE 185

Query: 184 ECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILVGM 243
           E K ++AEGDT+ T+VKFL  E SK +E AV++L+ELSKSEALCE+IGS++GAI++LVG+
Sbjct: 186 ESKAIVAEGDTVRTIVKFLSQEPSKGREAAVSVLFELSKSEALCEKIGSIHGAIILLVGL 245

Query: 244 SSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 290
           +SSKSEN+STVE AD+TL NLE  E N+RQMA  GRL+PLL ++LEG
Sbjct: 246 TSSKSENVSTVEKADKTLTNLERSEENVRQMAINGRLQPLLAKLLEG 285

BLAST of CSPI06G18550 vs. ExPASy Swiss-Prot
Match: Q10FT0 (U-box domain-containing protein 24 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.7e-67
Identity = 132/271 (48.71%), Postives = 190/271 (70.11%), Query Frame = 0

Query: 28  EPIYDSFLCPLTKQVMRDPVTIESGQTFERAAIEMWFNECKESRRRPICPMTLKELRSTE 87
           E  +++F+CPLTKQVMRDPVTIE+GQTFER AI  WF EC+++ RRP CP+T +ELR TE
Sbjct: 12  EGAFEAFVCPLTKQVMRDPVTIETGQTFEREAILKWFRECRDNGRRPTCPLTQRELRDTE 71

Query: 88  LNPSIALRNTIEEWTARNEAVQLDMARKSL--------NLSSPENETLGSLKYVQHVCQK 147
           ++PS+ALR+ I EW ARNE   LD A  SL             E   L +L +V  +CQ+
Sbjct: 72  VSPSVALRSVIHEWRARNEEKDLDRACASLVGGFAGHAGDEEEEESALRALVHVSQICQR 131

Query: 148 -GLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQEDSECKEMLAEGDTLHTVV 207
              S+ + R  G++  +  +LKS SR+++ ++L+ LR++ +++ + KE L +GDT+ T++
Sbjct: 132 SAASKDLVRRRGVLRAVAEMLKSGSRRLRLKSLQVLRVLVEDNDDNKEELGKGDTIRTII 191

Query: 208 KFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILVGMSSSKSENISTVENADR 267
           KFL +E  +E+E AV+LL+ELS  E  CE IG+V GAIL+LVGM SSKSE+   V+ A+ 
Sbjct: 192 KFLSNEHVQERELAVSLLHELSGHEPTCERIGAVYGAILLLVGMGSSKSESAVAVDKAES 251

Query: 268 TLENLEVCENNIRQMAEYGRLRPLLTQILEG 290
           TL NL+  + N++QMA+ GRL+PLLT++L G
Sbjct: 252 TLRNLDRFDANVKQMADNGRLQPLLTRLLRG 282

BLAST of CSPI06G18550 vs. ExPASy Swiss-Prot
Match: Q9CAA7 (Putative U-box domain-containing protein 42 OS=Arabidopsis thaliana OX=3702 GN=PUB42 PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.3e-43
Identity = 108/281 (38.43%), Postives = 169/281 (60.14%), Query Frame = 0

Query: 13  GSISDESSYYARL--HIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAIEMWFNECKES 72
           G++S+  S   ++   +EP Y +F+CPLTK++M DPVT E+G T ER A+  WF+    S
Sbjct: 227 GNLSESLSMLPQVTQFMEPPYQAFICPLTKEIMEDPVTTETGVTCERQAVIEWFDSFGNS 286

Query: 73  RRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLSSPENETLGSL 132
                CP+T ++L +TEL+ ++ L+  I+EW  RNEA ++ +A  +L+L   E+  + +L
Sbjct: 287 DEIN-CPVTGQKL-TTELSANVVLKTIIQEWKVRNEAARIKVAHAALSLGGSESMVIDAL 346

Query: 133 KYVQHVCQ-KGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQEDS-ECKEML 192
           + +Q  C+ K  ++   R AG+I ++   L   S+ V+F  L+ LR +A E++ + KEM+
Sbjct: 347 RDLQMTCEGKEYNKVQVREAGIIQLLDRYLTYRSKDVRFELLKFLRTLADEETDDGKEMI 406

Query: 193 AEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILVGMSSSKSE 252
            +  T+  V+K L       +  A ALL ELSKS+  CE+IG+  GAIL+LV    ++  
Sbjct: 407 VKTITMSCVIKLLGSSHQPVRHAAQALLLELSKSQHACEKIGTARGAILMLVTAKYNREL 466

Query: 253 NISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 290
           +    E +D+ L NLE C  NI+QMAE G L PLL  + EG
Sbjct: 467 DSFASETSDQILRNLEKCPENIKQMAESGLLEPLLGHLAEG 505

BLAST of CSPI06G18550 vs. ExPASy Swiss-Prot
Match: Q681N2 (U-box domain-containing protein 15 OS=Arabidopsis thaliana OX=3702 GN=PUB15 PE=2 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 1.9e-15
Identity = 74/266 (27.82%), Postives = 132/266 (49.62%), Query Frame = 0

Query: 34  FLCPLTKQVMRDPVTIESGQTFERAAIEMWFNECKESRRRPICPMTLKELRSTELNPSIA 93
           FLCP+T ++M DPV I +GQT+E+ +I+ WF+   ++     CP T +EL    L P+ A
Sbjct: 294 FLCPITLEIMLDPVIIATGQTYEKESIQKWFDAGHKT-----CPKTRQELDHLSLAPNFA 353

Query: 94  LRNTIEEWTARN--EAVQLDMARKSLN-------------LSSPENETLGSLKYVQHVCQ 153
           L+N I +W  +N  +  + +++  S N              SS   E   S+K ++ + +
Sbjct: 354 LKNLIMQWCEKNNFKIPEKEVSPDSQNEQKDEVSLLVEALSSSQLEEQRRSVKQMRLLAR 413

Query: 154 KG-LSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQEDSECKEMLAEGDTLHTV 213
           +   +R +  NAG IP++V LL      +Q  A+ TL +    D   K++++    +  +
Sbjct: 414 ENPENRVLIANAGAIPLLVQLLSYPDSGIQENAVTTL-LNLSIDEVNKKLISNEGAIPNI 473

Query: 214 VKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILVGMSSSKSENISTVENAD 273
           ++ L +   + +E + A L+ LS  +     IG  NG I  LV +   +   +   ++A 
Sbjct: 474 IEILENGNREARENSAAALFSLSMLDENKVTIGLSNG-IPPLVDL--LQHGTLRGKKDAL 533

Query: 274 RTLENLEVCENNIRQMAEYGRLRPLL 284
             L NL +   N  +  + G ++PLL
Sbjct: 534 TALFNLSLNSANKGRAIDAGIVQPLL 550

BLAST of CSPI06G18550 vs. ExPASy TrEMBL
Match: A0A0A0KGQ4 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G355460 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.4e-164
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 1   MAESWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI 60
           MAESWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI
Sbjct: 1   MAESWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI 60

Query: 61  EMWFNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLS 120
           EMWFNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLS
Sbjct: 61  EMWFNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLS 120

Query: 121 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE 180
           SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE
Sbjct: 121 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE 180

Query: 181 DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV 240
           DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV
Sbjct: 181 DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV 240

Query: 241 GMSSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEGMYVISSYRVLC 300
           GMSSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEGMYVISSYRVLC
Sbjct: 241 GMSSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEGMYVISSYRVLC 300

Query: 301 SY 303
           SY
Sbjct: 301 SY 302

BLAST of CSPI06G18550 vs. ExPASy TrEMBL
Match: A0A1S3CMH2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103502616 PE=4 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 3.2e-154
Identity = 284/289 (98.27%), Postives = 287/289 (99.31%), Query Frame = 0

Query: 1   MAESWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI 60
           MAESWDGSYEDSGS+SDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI
Sbjct: 1   MAESWDGSYEDSGSVSDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI 60

Query: 61  EMWFNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLS 120
           EMWFNECKESRRRPICPMTLKEL+STELNPSIALRNTIEEWTARNEAVQLD ARKSLNL 
Sbjct: 61  EMWFNECKESRRRPICPMTLKELKSTELNPSIALRNTIEEWTARNEAVQLDKARKSLNLG 120

Query: 121 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE 180
           SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE
Sbjct: 121 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE 180

Query: 181 DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV 240
           DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV
Sbjct: 181 DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV 240

Query: 241 GMSSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 290
           GMSSSKSENI+TVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG
Sbjct: 241 GMSSSKSENITTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 289

BLAST of CSPI06G18550 vs. ExPASy TrEMBL
Match: A0A5A7T682 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold119G00090 PE=4 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.6e-153
Identity = 283/288 (98.26%), Postives = 286/288 (99.31%), Query Frame = 0

Query: 1   MAESWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI 60
           MAESWDGSYEDSGS+SDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI
Sbjct: 23  MAESWDGSYEDSGSVSDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI 82

Query: 61  EMWFNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLS 120
           EMWFNECKESRRRPICPMTLKEL+STELNPSIALRNTIEEWTARNEAVQLD ARKSLNL 
Sbjct: 83  EMWFNECKESRRRPICPMTLKELKSTELNPSIALRNTIEEWTARNEAVQLDKARKSLNLG 142

Query: 121 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE 180
           SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE
Sbjct: 143 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE 202

Query: 181 DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV 240
           DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV
Sbjct: 203 DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV 262

Query: 241 GMSSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILE 289
           GMSSSKSENI+TVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILE
Sbjct: 263 GMSSSKSENITTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILE 310

BLAST of CSPI06G18550 vs. ExPASy TrEMBL
Match: A0A6J1KDM5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111493934 PE=4 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 2.7e-145
Identity = 265/289 (91.70%), Postives = 280/289 (96.89%), Query Frame = 0

Query: 1   MAESWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI 60
           MAESWDGSY+DSGS SDES +YARLHIEPIYDSFLCPLTK+VMRDPVTIESGQTFERAAI
Sbjct: 1   MAESWDGSYDDSGSFSDESGFYARLHIEPIYDSFLCPLTKKVMRDPVTIESGQTFERAAI 60

Query: 61  EMWFNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLS 120
           EMWFNEC+ESRR+PICPMTLKELR+T+LNPSIALRNTIEEWTARNEAVQLDMARKSLNL 
Sbjct: 61  EMWFNECRESRRKPICPMTLKELRTTDLNPSIALRNTIEEWTARNEAVQLDMARKSLNLG 120

Query: 121 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE 180
           SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSR+VQFRALETL+IV QE
Sbjct: 121 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRRVQFRALETLKIVVQE 180

Query: 181 DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV 240
           D+ECKEMLAEGDTL TVVKFLRHE SKEKEEAVALLYELS S+ LCE+IGSVNGAILILV
Sbjct: 181 DNECKEMLAEGDTLRTVVKFLRHEHSKEKEEAVALLYELSTSQDLCEKIGSVNGAILILV 240

Query: 241 GMSSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 290
           GMSSS+SENI+TVENA+RTLENLE CENNIRQMAEYGRLRPLLTQILEG
Sbjct: 241 GMSSSRSENITTVENAERTLENLETCENNIRQMAEYGRLRPLLTQILEG 289

BLAST of CSPI06G18550 vs. ExPASy TrEMBL
Match: A0A6J1G303 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111450328 PE=4 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 1.3e-144
Identity = 264/289 (91.35%), Postives = 279/289 (96.54%), Query Frame = 0

Query: 1   MAESWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI 60
           MAESWDGSY+DSGS SDES +YARLHIEPIYDSFLCPLTK+VMRDPVTIESGQTFERAAI
Sbjct: 1   MAESWDGSYDDSGSFSDESGFYARLHIEPIYDSFLCPLTKKVMRDPVTIESGQTFERAAI 60

Query: 61  EMWFNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLS 120
           EMWFNEC+ESRR+PICPMTLKELR+T+LNPSIALRNTIEEWTARNEAVQLDMARKSLNL 
Sbjct: 61  EMWFNECRESRRKPICPMTLKELRTTDLNPSIALRNTIEEWTARNEAVQLDMARKSLNLG 120

Query: 121 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE 180
           SPE ETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSR+VQFRALETL+IV QE
Sbjct: 121 SPEKETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRRVQFRALETLKIVVQE 180

Query: 181 DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV 240
           D+ECKEMLAEGDTL TVVKFLRHE SKEKEEAVALLYELS S+ LCE+IGSVNGAILILV
Sbjct: 181 DNECKEMLAEGDTLRTVVKFLRHEHSKEKEEAVALLYELSTSQDLCEKIGSVNGAILILV 240

Query: 241 GMSSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 290
           GMSSS+SENI+TVENA+RTLENLE CENNIRQMAEYGRLRPLLTQILEG
Sbjct: 241 GMSSSRSENITTVENAERTLENLETCENNIRQMAEYGRLRPLLTQILEG 289

BLAST of CSPI06G18550 vs. NCBI nr
Match: XP_004144243.1 (U-box domain-containing protein 44 [Cucumis sativus] >KAE8647205.1 hypothetical protein Csa_019081 [Cucumis sativus])

HSP 1 Score: 560.8 bits (1444), Expect = 7.0e-156
Identity = 289/289 (100.00%), Postives = 289/289 (100.00%), Query Frame = 0

Query: 1   MAESWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI 60
           MAESWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI
Sbjct: 1   MAESWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI 60

Query: 61  EMWFNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLS 120
           EMWFNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLS
Sbjct: 61  EMWFNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLS 120

Query: 121 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE 180
           SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE
Sbjct: 121 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE 180

Query: 181 DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV 240
           DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV
Sbjct: 181 DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV 240

Query: 241 GMSSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 290
           GMSSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG
Sbjct: 241 GMSSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 289

BLAST of CSPI06G18550 vs. NCBI nr
Match: XP_008464830.1 (PREDICTED: U-box domain-containing protein 44 [Cucumis melo])

HSP 1 Score: 554.3 bits (1427), Expect = 6.5e-154
Identity = 284/289 (98.27%), Postives = 287/289 (99.31%), Query Frame = 0

Query: 1   MAESWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI 60
           MAESWDGSYEDSGS+SDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI
Sbjct: 1   MAESWDGSYEDSGSVSDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI 60

Query: 61  EMWFNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLS 120
           EMWFNECKESRRRPICPMTLKEL+STELNPSIALRNTIEEWTARNEAVQLD ARKSLNL 
Sbjct: 61  EMWFNECKESRRRPICPMTLKELKSTELNPSIALRNTIEEWTARNEAVQLDKARKSLNLG 120

Query: 121 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE 180
           SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE
Sbjct: 121 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE 180

Query: 181 DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV 240
           DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV
Sbjct: 181 DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV 240

Query: 241 GMSSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 290
           GMSSSKSENI+TVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG
Sbjct: 241 GMSSSKSENITTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 289

BLAST of CSPI06G18550 vs. NCBI nr
Match: KAA0038443.1 (U-box domain-containing protein 44 [Cucumis melo var. makuwa])

HSP 1 Score: 552.0 bits (1421), Expect = 3.2e-153
Identity = 283/288 (98.26%), Postives = 286/288 (99.31%), Query Frame = 0

Query: 1   MAESWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI 60
           MAESWDGSYEDSGS+SDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI
Sbjct: 23  MAESWDGSYEDSGSVSDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI 82

Query: 61  EMWFNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLS 120
           EMWFNECKESRRRPICPMTLKEL+STELNPSIALRNTIEEWTARNEAVQLD ARKSLNL 
Sbjct: 83  EMWFNECKESRRRPICPMTLKELKSTELNPSIALRNTIEEWTARNEAVQLDKARKSLNLG 142

Query: 121 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE 180
           SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE
Sbjct: 143 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE 202

Query: 181 DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV 240
           DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV
Sbjct: 203 DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV 262

Query: 241 GMSSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILE 289
           GMSSSKSENI+TVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILE
Sbjct: 263 GMSSSKSENITTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILE 310

BLAST of CSPI06G18550 vs. NCBI nr
Match: XP_038884569.1 (U-box domain-containing protein 44-like [Benincasa hispida])

HSP 1 Score: 535.4 bits (1378), Expect = 3.1e-148
Identity = 273/287 (95.12%), Postives = 281/287 (97.91%), Query Frame = 0

Query: 3   ESWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAIEM 62
           ESWDGSY+DSGSISD+SSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAIEM
Sbjct: 6   ESWDGSYDDSGSISDDSSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAIEM 65

Query: 63  WFNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLSSP 122
           WF ECKESRRRPICPMTLKELR+T+LNPSIALRNTIEEWTARNEAVQLDMARKSLNL SP
Sbjct: 66  WFKECKESRRRPICPMTLKELRNTDLNPSIALRNTIEEWTARNEAVQLDMARKSLNLGSP 125

Query: 123 ENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQEDS 182
           E ETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIV QED+
Sbjct: 126 EKETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVVQEDN 185

Query: 183 ECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILVGM 242
           ECK++LAEGDTLHTVVKFLRHE SKEKEEAVALLYELSKSEALCEEIGSVNGAILILVGM
Sbjct: 186 ECKDILAEGDTLHTVVKFLRHEHSKEKEEAVALLYELSKSEALCEEIGSVNGAILILVGM 245

Query: 243 SSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 290
           SSSKSENISTVENADRTLENLE CENNIRQMAEYGRL+PLLTQILEG
Sbjct: 246 SSSKSENISTVENADRTLENLEKCENNIRQMAEYGRLKPLLTQILEG 292

BLAST of CSPI06G18550 vs. NCBI nr
Match: XP_023546186.1 (U-box domain-containing protein 44-like [Cucurbita pepo subsp. pepo] >XP_023546187.1 U-box domain-containing protein 44-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525.0 bits (1351), Expect = 4.3e-145
Identity = 264/289 (91.35%), Postives = 281/289 (97.23%), Query Frame = 0

Query: 1   MAESWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAI 60
           MAESWDGSY+DSGS SDES +YARLHIEPIYDSFLCPLTK+VMRDPVTIESGQTFERAAI
Sbjct: 1   MAESWDGSYDDSGSFSDESGFYARLHIEPIYDSFLCPLTKKVMRDPVTIESGQTFERAAI 60

Query: 61  EMWFNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLS 120
           EMWFNEC+ESRR+PICPMTLKELR+T+LNPSIALRNTIEEWTARNEAVQLDMARKSLNL 
Sbjct: 61  EMWFNECRESRRKPICPMTLKELRTTDLNPSIALRNTIEEWTARNEAVQLDMARKSLNLG 120

Query: 121 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQE 180
           SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSR+VQFRALETL+IV QE
Sbjct: 121 SPENETLGSLKYVQHVCQKGLSRHIARNAGLIPMIVSLLKSTSRRVQFRALETLKIVVQE 180

Query: 181 DSECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILV 240
           D+ECKEMLAEGDTL TVVKFLRHERSKEKEEAVALLYELS S+ LCE+IGSVNGAILILV
Sbjct: 181 DNECKEMLAEGDTLRTVVKFLRHERSKEKEEAVALLYELSTSQDLCEKIGSVNGAILILV 240

Query: 241 GMSSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 290
           GMSSS+SEN++TVENA+RTLENL+ CENNIRQMAEYGRLRPLLTQILEG
Sbjct: 241 GMSSSRSENVTTVENAERTLENLQTCENNIRQMAEYGRLRPLLTQILEG 289

BLAST of CSPI06G18550 vs. TAIR 10
Match: AT1G20780.1 (senescence-associated E3 ubiquitin ligase 1 )

HSP 1 Score: 317.8 bits (813), Expect = 9.6e-87
Identity = 160/278 (57.55%), Postives = 223/278 (80.22%), Query Frame = 0

Query: 13  GSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAIEMWFNECKESRR 72
           G  SD+SS++ R  ++ IY++F+CPLTK+VM DPVT+E+G+TFER AIE WF EC++S R
Sbjct: 7   GDQSDDSSHFER-GVDHIYEAFICPLTKEVMHDPVTLENGRTFEREAIEKWFKECRDSGR 66

Query: 73  RPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLSSPENETLGSLKY 132
            P CP+T +EL ST+++ SIALRNTIEEW +RN+A +LD+AR+SL L + E + L +L +
Sbjct: 67  PPSCPLTSQELTSTDVSASIALRNTIEEWRSRNDAAKLDIARQSLFLGNAETDILQALMH 126

Query: 133 VQHVCQKGLS-RHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQEDSECKEMLAEG 192
           V+ +C+   S RH  RN+ LI MI+ +LKSTS +V+++AL+TL++V + D E K ++AEG
Sbjct: 127 VRQICRTIRSNRHGVRNSQLIHMIIDMLKSTSHRVRYKALQTLQVVVEGDDESKAIVAEG 186

Query: 193 DTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILVGMSSSKSENIS 252
           DT+ T+VKFL HE SK +E AV+LL+ELSKSEALCE+IGS++GA+++LVG++SS SEN+S
Sbjct: 187 DTVRTLVKFLSHEPSKGREAAVSLLFELSKSEALCEKIGSIHGALILLVGLTSSNSENVS 246

Query: 253 TVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 290
            VE ADRTLEN+E  E  +RQMA YGRL+PLL ++LEG
Sbjct: 247 IVEKADRTLENMERSEEIVRQMASYGRLQPLLGKLLEG 283

BLAST of CSPI06G18550 vs. TAIR 10
Match: AT1G76390.1 (ARM repeat superfamily protein )

HSP 1 Score: 301.2 bits (770), Expect = 9.3e-82
Identity = 159/287 (55.40%), Postives = 216/287 (75.26%), Query Frame = 0

Query: 4   SWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAIEMW 63
           SWDGS  D+ S  +         I+ IY++F+CPLTKQVM +PVT+E+GQTFER AIE W
Sbjct: 6   SWDGSQSDNSSQFEPG-------IDNIYEAFICPLTKQVMHNPVTLENGQTFEREAIEKW 65

Query: 64  FNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLSSPE 123
           F EC+E+ +   CP+T KEL  T+L+PSIALRNTIEEW ARN+A++LD+AR+SL L + E
Sbjct: 66  FQECRENGQPLSCPITSKELSITDLSPSIALRNTIEEWRARNDALKLDIARQSLYLGNAE 125

Query: 124 NETLGSLKYVQHVCQK-GLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQEDS 183
              L +LK V+ +C+     R    N  L+ +I  +LKS+S +V+ +AL+TL++V + D 
Sbjct: 126 TNILLALKNVREICRNIRKIRQRVCNPQLVRLITDMLKSSSHEVRCKALQTLQVVVEGDE 185

Query: 184 ECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILVGM 243
           E K ++AEGDT+ T+VKFL  E SK +E AV++L+ELSKSEALCE+IGS++GAI++LVG+
Sbjct: 186 ESKAIVAEGDTVRTIVKFLSQEPSKGREAAVSVLFELSKSEALCEKIGSIHGAIILLVGL 245

Query: 244 SSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 290
           +SSKSEN+STVE AD+TL NLE  E N+RQMA  GRL+PLL ++LEG
Sbjct: 246 TSSKSENVSTVEKADKTLTNLERSEENVRQMAINGRLQPLLAKLLEG 285

BLAST of CSPI06G18550 vs. TAIR 10
Match: AT1G76390.2 (ARM repeat superfamily protein )

HSP 1 Score: 301.2 bits (770), Expect = 9.3e-82
Identity = 159/287 (55.40%), Postives = 216/287 (75.26%), Query Frame = 0

Query: 4   SWDGSYEDSGSISDESSYYARLHIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAIEMW 63
           SWDGS  D+ S  +         I+ IY++F+CPLTKQVM +PVT+E+GQTFER AIE W
Sbjct: 6   SWDGSQSDNSSQFEPG-------IDNIYEAFICPLTKQVMHNPVTLENGQTFEREAIEKW 65

Query: 64  FNECKESRRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLSSPE 123
           F EC+E+ +   CP+T KEL  T+L+PSIALRNTIEEW ARN+A++LD+AR+SL L + E
Sbjct: 66  FQECRENGQPLSCPITSKELSITDLSPSIALRNTIEEWRARNDALKLDIARQSLYLGNAE 125

Query: 124 NETLGSLKYVQHVCQK-GLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQEDS 183
              L +LK V+ +C+     R    N  L+ +I  +LKS+S +V+ +AL+TL++V + D 
Sbjct: 126 TNILLALKNVREICRNIRKIRQRVCNPQLVRLITDMLKSSSHEVRCKALQTLQVVVEGDE 185

Query: 184 ECKEMLAEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILVGM 243
           E K ++AEGDT+ T+VKFL  E SK +E AV++L+ELSKSEALCE+IGS++GAI++LVG+
Sbjct: 186 ESKAIVAEGDTVRTIVKFLSQEPSKGREAAVSVLFELSKSEALCEKIGSIHGAIILLVGL 245

Query: 244 SSSKSENISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 290
           +SSKSEN+STVE AD+TL NLE  E N+RQMA  GRL+PLL ++LEG
Sbjct: 246 TSSKSENVSTVEKADKTLTNLERSEENVRQMAINGRLQPLLAKLLEG 285

BLAST of CSPI06G18550 vs. TAIR 10
Match: AT1G68940.1 (Armadillo/beta-catenin-like repeat family protein )

HSP 1 Score: 178.3 bits (451), Expect = 9.1e-45
Identity = 108/281 (38.43%), Postives = 169/281 (60.14%), Query Frame = 0

Query: 13  GSISDESSYYARL--HIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAIEMWFNECKES 72
           G++S+  S   ++   +EP Y +F+CPLTK++M DPVT E+G T ER A+  WF+    S
Sbjct: 227 GNLSESLSMLPQVTQFMEPPYQAFICPLTKEIMEDPVTTETGVTCERQAVIEWFDSFGNS 286

Query: 73  RRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLSSPENETLGSL 132
                CP+T ++L +TEL+ ++ L+  I+EW  RNEA ++ +A  +L+L   E+  + +L
Sbjct: 287 DEIN-CPVTGQKL-TTELSANVVLKTIIQEWKVRNEAARIKVAHAALSLGGSESMVIDAL 346

Query: 133 KYVQHVCQ-KGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQEDS-ECKEML 192
           + +Q  C+ K  ++   R AG+I ++   L   S+ V+F  L+ LR +A E++ + KEM+
Sbjct: 347 RDLQMTCEGKEYNKVQVREAGIIQLLDRYLTYRSKDVRFELLKFLRTLADEETDDGKEMI 406

Query: 193 AEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILVGMSSSKSE 252
            +  T+  V+K L       +  A ALL ELSKS+  CE+IG+  GAIL+LV    ++  
Sbjct: 407 VKTITMSCVIKLLGSSHQPVRHAAQALLLELSKSQHACEKIGTARGAILMLVTAKYNREL 466

Query: 253 NISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 290
           +    E +D+ L NLE C  NI+QMAE G L PLL  + EG
Sbjct: 467 DSFASETSDQILRNLEKCPENIKQMAESGLLEPLLGHLAEG 505

BLAST of CSPI06G18550 vs. TAIR 10
Match: AT1G68940.2 (Armadillo/beta-catenin-like repeat family protein )

HSP 1 Score: 178.3 bits (451), Expect = 9.1e-45
Identity = 108/281 (38.43%), Postives = 169/281 (60.14%), Query Frame = 0

Query: 13  GSISDESSYYARL--HIEPIYDSFLCPLTKQVMRDPVTIESGQTFERAAIEMWFNECKES 72
           G++S+  S   ++   +EP Y +F+CPLTK++M DPVT E+G T ER A+  WF+    S
Sbjct: 227 GNLSESLSMLPQVTQFMEPPYQAFICPLTKEIMEDPVTTETGVTCERQAVIEWFDSFGNS 286

Query: 73  RRRPICPMTLKELRSTELNPSIALRNTIEEWTARNEAVQLDMARKSLNLSSPENETLGSL 132
                CP+T ++L +TEL+ ++ L+  I+EW  RNEA ++ +A  +L+L   E+  + +L
Sbjct: 287 DEIN-CPVTGQKL-TTELSANVVLKTIIQEWKVRNEAARIKVAHAALSLGGSESMVIDAL 346

Query: 133 KYVQHVCQ-KGLSRHIARNAGLIPMIVSLLKSTSRKVQFRALETLRIVAQEDS-ECKEML 192
           + +Q  C+ K  ++   R AG+I ++   L   S+ V+F  L+ LR +A E++ + KEM+
Sbjct: 347 RDLQMTCEGKEYNKVQVREAGIIQLLDRYLTYRSKDVRFELLKFLRTLADEETDDGKEMI 406

Query: 193 AEGDTLHTVVKFLRHERSKEKEEAVALLYELSKSEALCEEIGSVNGAILILVGMSSSKSE 252
            +  T+  V+K L       +  A ALL ELSKS+  CE+IG+  GAIL+LV    ++  
Sbjct: 407 VKTITMSCVIKLLGSSHQPVRHAAQALLLELSKSQHACEKIGTARGAILMLVTAKYNREL 466

Query: 253 NISTVENADRTLENLEVCENNIRQMAEYGRLRPLLTQILEG 290
           +    E +D+ L NLE C  NI+QMAE G L PLL  + EG
Sbjct: 467 DSFASETSDQILRNLEKCPENIKQMAESGLLEPLLGHLAEG 505

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LM761.4e-8557.55U-box domain-containing protein 44 OS=Arabidopsis thaliana OX=3702 GN=PUB44 PE=1... [more]
Q9SFX21.3e-8055.40U-box domain-containing protein 43 OS=Arabidopsis thaliana OX=3702 GN=PUB43 PE=2... [more]
Q10FT01.7e-6748.71U-box domain-containing protein 24 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Q9CAA71.3e-4338.43Putative U-box domain-containing protein 42 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q681N21.9e-1527.82U-box domain-containing protein 15 OS=Arabidopsis thaliana OX=3702 GN=PUB15 PE=2... [more]
Match NameE-valueIdentityDescription
A0A0A0KGQ44.4e-164100.00RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G355460 PE... [more]
A0A1S3CMH23.2e-15498.27RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103502616 PE=4 ... [more]
A0A5A7T6821.6e-15398.26RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A6J1KDM52.7e-14591.70RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111493934 P... [more]
A0A6J1G3031.3e-14491.35RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111450328... [more]
Match NameE-valueIdentityDescription
XP_004144243.17.0e-156100.00U-box domain-containing protein 44 [Cucumis sativus] >KAE8647205.1 hypothetical ... [more]
XP_008464830.16.5e-15498.27PREDICTED: U-box domain-containing protein 44 [Cucumis melo][more]
KAA0038443.13.2e-15398.26U-box domain-containing protein 44 [Cucumis melo var. makuwa][more]
XP_038884569.13.1e-14895.12U-box domain-containing protein 44-like [Benincasa hispida][more]
XP_023546186.14.3e-14591.35U-box domain-containing protein 44-like [Cucurbita pepo subsp. pepo] >XP_0235461... [more]
Match NameE-valueIdentityDescription
AT1G20780.19.6e-8757.55senescence-associated E3 ubiquitin ligase 1 [more]
AT1G76390.19.3e-8255.40ARM repeat superfamily protein [more]
AT1G76390.29.3e-8255.40ARM repeat superfamily protein [more]
AT1G68940.19.1e-4538.43Armadillo/beta-catenin-like repeat family protein [more]
AT1G68940.29.1e-4538.43Armadillo/beta-catenin-like repeat family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000225ArmadilloSMARTSM00185arm_5coord: 181..221
e-value: 38.0
score: 8.8
coord: 139..179
e-value: 0.93
score: 18.5
IPR003613U box domainSMARTSM00504Ubox_2coord: 33..101
e-value: 2.3E-25
score: 100.2
IPR003613U box domainPFAMPF04564U-boxcoord: 32..106
e-value: 4.7E-16
score: 58.7
IPR003613U box domainPROSITEPS51698U_BOXcoord: 29..108
score: 23.575556
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 105..299
e-value: 2.3E-23
score: 84.5
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 29..104
e-value: 5.2E-23
score: 82.8
NoneNo IPR availablePANTHERPTHR45958:SF6U-BOX DOMAIN-CONTAINING PROTEIN 43coord: 1..290
NoneNo IPR availablePANTHERPTHR45958RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 1..290
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 34..79
e-value: 1.04364E-18
score: 75.7331
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 18..111
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 110..284

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G18550.1CSPI06G18550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity