CSPI05G01660 (gene) Cucumber (PI 183967) v1

BLAST of CSPI05G01660 vs. ExPASy TrEMBL
Match: A0A5A7UN97 (Putative beta-tubulin polyglutamylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00410 PE=4 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.3e-160
Identity = 327/385 (84.94%), Postives = 348/385 (90.39%), Query Frame = 0

Query: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKA 60
           MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMK 
Sbjct: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKV 60

Query: 61  EVISSEIPRIALRTSTRRKAVKDETITTRSRQGAVARDMEESEDKDLNMALTTPSLPGSW 120
           EVIS EIPR ALRTSTRRKAVKDETIT RSR+GAVAR  EESE++DLNMALTTPSLPGS 
Sbjct: 61  EVISLEIPRTALRTSTRRKAVKDETITIRSRRGAVARGTEESENRDLNMALTTPSLPGSR 120

Query: 121 RRTAAASSAC*KFDFQMTVDDQKEDKDMDQEKKEIENTPAVPKSQKRVAGASTRKRTETK 180
           RRTAAASSAC K DFQMT+DDQKED D+DQEKKEIE TPAVPKSQKRV GASTRKRTETK
Sbjct: 121 RRTAAASSACKKVDFQMTIDDQKEDNDLDQEKKEIEKTPAVPKSQKRVVGASTRKRTETK 180

Query: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLEGDEKMEAITVHMPFDDMPKISEPMKENMELE 240
           NNGAAEQRVYSTRRSVRLLEKNMESLSL GDE+ME I+VHM F+DMP  S P+KEN +LE
Sbjct: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLGGDEEMEPISVHMSFEDMPNSSVPVKENTKLE 240

Query: 241 TESKKTDESKSKLDENLSVEVDDGEKNEMGSEVKLSDEEPEMVLDDLLKSFDENVISAKS 300
            ESK  DES+SKLDE+L VEVDDGEK+E+GSEVKLS+EEPE+VLDDLLKS DENV++AKS
Sbjct: 241 IESKIIDESESKLDEDLRVEVDDGEKSEIGSEVKLSEEEPEIVLDDLLKSSDENVVTAKS 300

Query: 301 VDDSKIEAAHANSDVKCFSEDEKVNEVSKDDDFVEISAEEINESDKSSLPTEDLDVTE-V 360
           VDDS+ EA HAN DVKCFSED KVNE SK    VEISAEEINESDKSSLPTEDLDVTE V
Sbjct: 301 VDDSETEALHANLDVKCFSED-KVNEASK---AVEISAEEINESDKSSLPTEDLDVTEVV 360

Query: 361 HKSSIAEEFGMEFESQQNESTCELE 385
           HKS+IAEEFGME E +QNEST ELE
Sbjct: 361 HKSNIAEEFGMEVECKQNESTSELE 381

BLAST of CSPI05G01660 vs. ExPASy TrEMBL
Match: A0A1S3CT90 (probable beta-tubulin polyglutamylase OS=Cucumis melo OX=3656 GN=LOC103504683 PE=4 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 3.7e-160
Identity = 327/385 (84.94%), Postives = 347/385 (90.13%), Query Frame = 0

Query: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKA 60
           MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGV ESPMK 
Sbjct: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVSESPMKV 60

Query: 61  EVISSEIPRIALRTSTRRKAVKDETITTRSRQGAVARDMEESEDKDLNMALTTPSLPGSW 120
           EVIS EIPR ALRTSTRRKAVKDETITTRSR+GAVAR  EESE++D NMALTTPSLPGS 
Sbjct: 61  EVISLEIPRTALRTSTRRKAVKDETITTRSRRGAVARGTEESENRDFNMALTTPSLPGSR 120

Query: 121 RRTAAASSAC*KFDFQMTVDDQKEDKDMDQEKKEIENTPAVPKSQKRVAGASTRKRTETK 180
           RRTAAASSAC K DFQMTVDDQKED D+DQEKKEIE TPAVPKSQKRV GASTRKRTET+
Sbjct: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDQEKKEIEKTPAVPKSQKRVVGASTRKRTETR 180

Query: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLEGDEKMEAITVHMPFDDMPKISEPMKENMELE 240
           NNGAAEQRVYSTRRSVRLLEKNMESLSL GDE+ME I+VHM F+DMP  S P+KEN ELE
Sbjct: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLGGDEEMEPISVHMSFEDMPNSSVPVKENTELE 240

Query: 241 TESKKTDESKSKLDENLSVEVDDGEKNEMGSEVKLSDEEPEMVLDDLLKSFDENVISAKS 300
            ESK  DES+SKLDE+L VEVDDGEK+E+GSEVKLS+EEPE+VLDDLLKS DENV++AKS
Sbjct: 241 IESKIIDESESKLDEDLRVEVDDGEKSEIGSEVKLSEEEPEIVLDDLLKSSDENVVTAKS 300

Query: 301 VDDSKIEAAHANSDVKCFSEDEKVNEVSKDDDFVEISAEEINESDKSSLPTEDLDVTE-V 360
           VDDS+ EA HAN DVKCFSED KVNE SK    VEISAEEINESDKSSLPTEDLDVTE V
Sbjct: 301 VDDSETEALHANLDVKCFSED-KVNEASK---AVEISAEEINESDKSSLPTEDLDVTEVV 360

Query: 361 HKSSIAEEFGMEFESQQNESTCELE 385
           HKS+IAEEFGME E +QNEST ELE
Sbjct: 361 HKSNIAEEFGMEVECKQNESTSELE 381

BLAST of CSPI05G01660 vs. ExPASy TrEMBL
Match: A0A0A0KJF8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G077220 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 8.7e-133
Identity = 263/270 (97.41%), Postives = 266/270 (98.52%), Query Frame = 0

Query: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKA 60
           MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKA
Sbjct: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKA 60

Query: 61  EVISSEIPRIALRTSTRRKAVKDETITTRSRQGAVARDMEESEDKDLNMALTTPSLPGSW 120
            VISSEIPRIALRTSTRRKAVKDETITTRSRQGAVARDMEESEDKDLNMALTTPSLPGS 
Sbjct: 61  GVISSEIPRIALRTSTRRKAVKDETITTRSRQGAVARDMEESEDKDLNMALTTPSLPGSR 120

Query: 121 RRTAAASSAC*KFDFQMTVDDQKEDKDMDQEKKEIENTPAVPKSQKRVAGASTRKRTETK 180
           RRTAAASSAC KFDFQMTVDDQKEDKDMDQ+KKEIENTPAVPKSQKRVAGASTRKRTETK
Sbjct: 121 RRTAAASSACKKFDFQMTVDDQKEDKDMDQKKKEIENTPAVPKSQKRVAGASTRKRTETK 180

Query: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLEGDEKMEAITVHMPFDDMPKISEPMKENMELE 240
           ++GAAEQRVYSTRRSVRLLEKNMESLSLEGDEKME ITVHMPFDDMPKISEPMKENMELE
Sbjct: 181 DSGAAEQRVYSTRRSVRLLEKNMESLSLEGDEKMEPITVHMPFDDMPKISEPMKENMELE 240

Query: 241 TESKKTDESKSKLDENLSVEVDDGEKNEMG 271
           TESKKTDESKSKLDENLSVEVDDGEKNEMG
Sbjct: 241 TESKKTDESKSKLDENLSVEVDDGEKNEMG 270

BLAST of CSPI05G01660 vs. ExPASy TrEMBL
Match: A0A0A0KKZ3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G169010 PE=4 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 3.1e-114
Identity = 232/256 (90.62%), Postives = 242/256 (94.53%), Query Frame = 0

Query: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKA 60
           MDF+TLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFL+GDRSGVPESPMK 
Sbjct: 1   MDFYTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLDGDRSGVPESPMKG 60

Query: 61  -EVISSEIPRIALRTSTRRKAVKDETITTRSRQGAVARDMEESEDKDLNMALTTPSLPGS 120
            EV+SSEI R ALRTSTRRKAVKDETITTRSR+GA ARD EESE++DLNMA TTPSLPGS
Sbjct: 61  EEVVSSEIARTALRTSTRRKAVKDETITTRSRRGAAARDTEESENRDLNMAFTTPSLPGS 120

Query: 121 WRRTAAASSAC*KFDFQMTVDDQKEDKDMDQEKKEIENTPAVPKSQKRVAGASTRKRTET 180
            RRTAAASSAC K DFQMTVDDQKEDKD+DQEKKEIENTPAVPKSQKRVAGASTRKRTET
Sbjct: 121 RRRTAAASSACKKVDFQMTVDDQKEDKDLDQEKKEIENTPAVPKSQKRVAGASTRKRTET 180

Query: 181 KNNGAAEQRVYSTRRSVRLLEKNMESLSLEGDEKMEAITVHMPFDDMPKISEPMKENMEL 240
           K++GAAEQRVYSTRRSVRLLEKNMESLSLEGDEKME ITVHM FDDMP ISEP+KENMEL
Sbjct: 181 KDSGAAEQRVYSTRRSVRLLEKNMESLSLEGDEKMEPITVHMSFDDMPNISEPVKENMEL 240

Query: 241 ETESKKTDESKSKLDE 256
           ETESKKTDES SKLDE
Sbjct: 241 ETESKKTDESVSKLDE 256

BLAST of CSPI05G01660 vs. ExPASy TrEMBL
Match: A0A6J1HZT2 (uncharacterized protein LOC111469129 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469129 PE=4 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 8.2e-107
Identity = 238/384 (61.98%), Postives = 275/384 (71.61%), Query Frame = 0

Query: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKA 60
           MDFH+LSRRELQ LCKRNKIPANITNVAMADALAAL  VEGIEEFL+ +RSGVPESPMK 
Sbjct: 1   MDFHSLSRRELQTLCKRNKIPANITNVAMADALAALPLVEGIEEFLSSERSGVPESPMKG 60

Query: 61  EVISSEIPRIALRTSTRRKAVKDETITTRSRQGAVARDMEESEDKDLNMALTTPSLPGSW 120
           EV+S EIPR  LRTSTRRK VKD+ ITT +R+ A AR  EESE++DLN+ALTTPSL    
Sbjct: 61  EVVSFEIPRTELRTSTRRKTVKDDPITTLTRRAAAARGTEESENRDLNVALTTPSLRSGR 120

Query: 121 RRTAAASSAC*KFDFQMTVDDQKEDKDMDQEKKEIENTPAVPKSQKRVAGASTRKRTETK 180
           RRTA ASSAC K DFQM VDD++E+ D+D EK  IENTPA PKSQ+ VAG ST KRT+T+
Sbjct: 121 RRTAGASSACKKVDFQMAVDDEEEENDLDHEKPVIENTPAAPKSQRSVAGPSTSKRTQTR 180

Query: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLEGDEKMEAITVHMPFDDMPKISEPMKENMELE 240
           NNG  EQRVYSTRRSVRLLE+ MESLSL  D KM+ ITVHM FD++P  S P+KE  +LE
Sbjct: 181 NNGVVEQRVYSTRRSVRLLEQTMESLSLAEDRKMKPITVHMSFDEIPNSSGPVKEETKLE 240

Query: 241 TESKKTDESKSKLDENLSVEVDDGEKNEMGSEVKLSDEEPEMVLDDLLKSFDENVISAKS 300
            ESKKTDES+SKLDE+L +E ++ EKNEM        EEP+M  DD              
Sbjct: 241 VESKKTDESESKLDEDLGLETNEQEKNEM--------EEPQMDPDD-------------- 300

Query: 301 VDDSKIEAAHANSDVKCFSEDEKVNEVSKDDDFVEISAEEINESDKSSLPTEDLDVTEVH 360
                         +KC +            D VEISAEEIN+SDK SLP+ED+D+TEVH
Sbjct: 301 -------------SMKCSA------------DSVEISAEEINDSDKLSLPSEDVDLTEVH 337

Query: 361 KSSIAEEFGMEFESQQNESTCELE 385
            SSI EE GME +S+ NES   LE
Sbjct: 361 HSSIEEESGMEVQSKYNESIFALE 337

BLAST of CSPI05G01660 vs. NCBI nr
Match: XP_031740824.1 (midasin-like [Cucumis sativus])

HSP 1 Score: 683.3 bits (1762), Expect = 1.2e-192
Identity = 375/384 (97.66%), Postives = 377/384 (98.18%), Query Frame = 0

Query: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKA 60
           MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKA
Sbjct: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKA 60

Query: 61  EVISSEIPRIALRTSTRRKAVKDETITTRSRQGAVARDMEESEDKDLNMALTTPSLPGSW 120
            VISSEIPRIALRTSTRRKAVKDETITTRSRQGAVARDMEESEDKDLNMALTTPSLPGS 
Sbjct: 61  GVISSEIPRIALRTSTRRKAVKDETITTRSRQGAVARDMEESEDKDLNMALTTPSLPGSR 120

Query: 121 RRTAAASSAC*KFDFQMTVDDQKEDKDMDQEKKEIENTPAVPKSQKRVAGASTRKRTETK 180
           RRTAAASSAC KFDFQMTVDDQKEDKDMDQ+KKEIENTPAVPKSQKRV GASTRKRTETK
Sbjct: 121 RRTAAASSACKKFDFQMTVDDQKEDKDMDQKKKEIENTPAVPKSQKRVVGASTRKRTETK 180

Query: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLEGDEKMEAITVHMPFDDMPKISEPMKENMELE 240
           NNGAAEQRVYSTRRSVRLLEKNMESLSLEGDEKME ITVHMPFDDMPKISEPMKENMELE
Sbjct: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLEGDEKMEPITVHMPFDDMPKISEPMKENMELE 240

Query: 241 TESKKTDESKSKLDENLSVEVDDGEKNEMGSEVKLSDEEPEMVLDDLLKSFDENVISAKS 300
           TESKKTDESKSKLDENLSVEVDDGEKNEMGSEVKLS+EEPEMVLDDLLKSFDENVISAKS
Sbjct: 241 TESKKTDESKSKLDENLSVEVDDGEKNEMGSEVKLSEEEPEMVLDDLLKSFDENVISAKS 300

Query: 301 VDDSKIEAAHANSDVKCFSEDEKVNEVSKDDDFVEISAEEINESDKSSLPTEDLDVTEVH 360
           VDDSKIEA HANSDVKCFSEDEKVNEVSKDDD VEISAEEINESDKSSLPTEDLDVTEVH
Sbjct: 301 VDDSKIEAPHANSDVKCFSEDEKVNEVSKDDDSVEISAEEINESDKSSLPTEDLDVTEVH 360

Query: 361 KSSIAEEFGMEFESQQNESTCELE 385
           KSSIAEEFGMEFESQQNESTCELE
Sbjct: 361 KSSIAEEFGMEFESQQNESTCELE 384

BLAST of CSPI05G01660 vs. NCBI nr
Match: XP_011654865.2 (probable serine/threonine-protein kinase kinX isoform X2 [Cucumis sativus] >KAE8647937.1 hypothetical protein Csa_000497 [Cucumis sativus])

HSP 1 Score: 639.0 bits (1647), Expect = 2.6e-179
Identity = 352/384 (91.67%), Postives = 366/384 (95.31%), Query Frame = 0

Query: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKA 60
           MDF+TLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMK 
Sbjct: 1   MDFYTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKG 60

Query: 61  EVISSEIPRIALRTSTRRKAVKDETITTRSRQGAVARDMEESEDKDLNMALTTPSLPGSW 120
           EVISSEIPR ALRTSTRRKAVKDETITTRSR+GA ARD EESE++DLNMALTTPSLPGS 
Sbjct: 61  EVISSEIPRTALRTSTRRKAVKDETITTRSRRGAAARDTEESENRDLNMALTTPSLPGSR 120

Query: 121 RRTAAASSAC*KFDFQMTVDDQKEDKDMDQEKKEIENTPAVPKSQKRVAGASTRKRTETK 180
           RRTAAASSAC K DFQMTVDDQKEDKD+DQEKKEIENTPAVPKSQKRVAGASTRKRTETK
Sbjct: 121 RRTAAASSACKKVDFQMTVDDQKEDKDLDQEKKEIENTPAVPKSQKRVAGASTRKRTETK 180

Query: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLEGDEKMEAITVHMPFDDMPKISEPMKENMELE 240
           ++GAAEQRVYSTRRSVRLLEKNMES+SL GDEKME ITVHM FDDMP ISEP+KENMELE
Sbjct: 181 DSGAAEQRVYSTRRSVRLLEKNMESMSLGGDEKMEPITVHMSFDDMPNISEPVKENMELE 240

Query: 241 TESKKTDESKSKLDENLSVEVDDGEKNEMGSEVKLSDEEPEMVLDDLLKSFDENVISAKS 300
           TESKKTDES SKLDE+LSVEVDDGEKNEMG+EVKLS+EEPE+VLDDLLKSFDENVISAKS
Sbjct: 241 TESKKTDESVSKLDEDLSVEVDDGEKNEMGTEVKLSEEEPEIVLDDLLKSFDENVISAKS 300

Query: 301 VDDSKIEAAHANSDVKCFSEDEKVNEVSKDDDFVEISAEEINESDKSSLPTEDLDVTEVH 360
           VDDS  EA HANSDVKCFSEDEKVNEVSKDDD VE SAEEINESDKSSLPTEDLDVTEVH
Sbjct: 301 VDDSITEAPHANSDVKCFSEDEKVNEVSKDDDSVESSAEEINESDKSSLPTEDLDVTEVH 360

Query: 361 KSSIAEEFGMEFESQQNESTCELE 385
           KSSIAEEFG EFESQQNESTCEL+
Sbjct: 361 KSSIAEEFGKEFESQQNESTCELD 384

BLAST of CSPI05G01660 vs. NCBI nr
Match: XP_031742149.1 (probable serine/threonine-protein kinase kinX isoform X1 [Cucumis sativus])

HSP 1 Score: 633.3 bits (1632), Expect = 1.4e-177
Identity = 352/388 (90.72%), Postives = 366/388 (94.33%), Query Frame = 0

Query: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKA 60
           MDF+TLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMK 
Sbjct: 1   MDFYTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKG 60

Query: 61  EVISSEIPRIALRTSTRRKAVKDETITTRSRQGAVARDMEESEDKDLNMALTTPSLPGSW 120
           EVISSEIPR ALRTSTRRKAVKDETITTRSR+GA ARD EESE++DLNMALTTPSLPGS 
Sbjct: 61  EVISSEIPRTALRTSTRRKAVKDETITTRSRRGAAARDTEESENRDLNMALTTPSLPGSR 120

Query: 121 RRTAAASSAC*KFDFQMTVDDQKEDKDMDQEKKEIENTPAVPKSQKRVAGASTRKRTETK 180
           RRTAAASSAC K DFQMTVDDQKEDKD+DQEKKEIENTPAVPKSQKRVAGASTRKRTETK
Sbjct: 121 RRTAAASSACKKVDFQMTVDDQKEDKDLDQEKKEIENTPAVPKSQKRVAGASTRKRTETK 180

Query: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLEGDEKMEAITVHMPFDDMPKIS----EPMKEN 240
           ++GAAEQRVYSTRRSVRLLEKNMES+SL GDEKME ITVHM FDDMP IS    EP+KEN
Sbjct: 181 DSGAAEQRVYSTRRSVRLLEKNMESMSLGGDEKMEPITVHMSFDDMPNISAIGAEPVKEN 240

Query: 241 MELETESKKTDESKSKLDENLSVEVDDGEKNEMGSEVKLSDEEPEMVLDDLLKSFDENVI 300
           MELETESKKTDES SKLDE+LSVEVDDGEKNEMG+EVKLS+EEPE+VLDDLLKSFDENVI
Sbjct: 241 MELETESKKTDESVSKLDEDLSVEVDDGEKNEMGTEVKLSEEEPEIVLDDLLKSFDENVI 300

Query: 301 SAKSVDDSKIEAAHANSDVKCFSEDEKVNEVSKDDDFVEISAEEINESDKSSLPTEDLDV 360
           SAKSVDDS  EA HANSDVKCFSEDEKVNEVSKDDD VE SAEEINESDKSSLPTEDLDV
Sbjct: 301 SAKSVDDSITEAPHANSDVKCFSEDEKVNEVSKDDDSVESSAEEINESDKSSLPTEDLDV 360

Query: 361 TEVHKSSIAEEFGMEFESQQNESTCELE 385
           TEVHKSSIAEEFG EFESQQNESTCEL+
Sbjct: 361 TEVHKSSIAEEFGKEFESQQNESTCELD 388

BLAST of CSPI05G01660 vs. NCBI nr
Match: KAA0055115.1 (putative beta-tubulin polyglutamylase [Cucumis melo var. makuwa])

HSP 1 Score: 575.9 bits (1483), Expect = 2.7e-160
Identity = 327/385 (84.94%), Postives = 348/385 (90.39%), Query Frame = 0

Query: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKA 60
           MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMK 
Sbjct: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKV 60

Query: 61  EVISSEIPRIALRTSTRRKAVKDETITTRSRQGAVARDMEESEDKDLNMALTTPSLPGSW 120
           EVIS EIPR ALRTSTRRKAVKDETIT RSR+GAVAR  EESE++DLNMALTTPSLPGS 
Sbjct: 61  EVISLEIPRTALRTSTRRKAVKDETITIRSRRGAVARGTEESENRDLNMALTTPSLPGSR 120

Query: 121 RRTAAASSAC*KFDFQMTVDDQKEDKDMDQEKKEIENTPAVPKSQKRVAGASTRKRTETK 180
           RRTAAASSAC K DFQMT+DDQKED D+DQEKKEIE TPAVPKSQKRV GASTRKRTETK
Sbjct: 121 RRTAAASSACKKVDFQMTIDDQKEDNDLDQEKKEIEKTPAVPKSQKRVVGASTRKRTETK 180

Query: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLEGDEKMEAITVHMPFDDMPKISEPMKENMELE 240
           NNGAAEQRVYSTRRSVRLLEKNMESLSL GDE+ME I+VHM F+DMP  S P+KEN +LE
Sbjct: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLGGDEEMEPISVHMSFEDMPNSSVPVKENTKLE 240

Query: 241 TESKKTDESKSKLDENLSVEVDDGEKNEMGSEVKLSDEEPEMVLDDLLKSFDENVISAKS 300
            ESK  DES+SKLDE+L VEVDDGEK+E+GSEVKLS+EEPE+VLDDLLKS DENV++AKS
Sbjct: 241 IESKIIDESESKLDEDLRVEVDDGEKSEIGSEVKLSEEEPEIVLDDLLKSSDENVVTAKS 300

Query: 301 VDDSKIEAAHANSDVKCFSEDEKVNEVSKDDDFVEISAEEINESDKSSLPTEDLDVTE-V 360
           VDDS+ EA HAN DVKCFSED KVNE SK    VEISAEEINESDKSSLPTEDLDVTE V
Sbjct: 301 VDDSETEALHANLDVKCFSED-KVNEASK---AVEISAEEINESDKSSLPTEDLDVTEVV 360

Query: 361 HKSSIAEEFGMEFESQQNESTCELE 385
           HKS+IAEEFGME E +QNEST ELE
Sbjct: 361 HKSNIAEEFGMEVECKQNESTSELE 381

BLAST of CSPI05G01660 vs. NCBI nr
Match: XP_008467303.1 (PREDICTED: probable beta-tubulin polyglutamylase [Cucumis melo])

HSP 1 Score: 574.3 bits (1479), Expect = 7.7e-160
Identity = 327/385 (84.94%), Postives = 347/385 (90.13%), Query Frame = 0

Query: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKA 60
           MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGV ESPMK 
Sbjct: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVSESPMKV 60

Query: 61  EVISSEIPRIALRTSTRRKAVKDETITTRSRQGAVARDMEESEDKDLNMALTTPSLPGSW 120
           EVIS EIPR ALRTSTRRKAVKDETITTRSR+GAVAR  EESE++D NMALTTPSLPGS 
Sbjct: 61  EVISLEIPRTALRTSTRRKAVKDETITTRSRRGAVARGTEESENRDFNMALTTPSLPGSR 120

Query: 121 RRTAAASSAC*KFDFQMTVDDQKEDKDMDQEKKEIENTPAVPKSQKRVAGASTRKRTETK 180
           RRTAAASSAC K DFQMTVDDQKED D+DQEKKEIE TPAVPKSQKRV GASTRKRTET+
Sbjct: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDQEKKEIEKTPAVPKSQKRVVGASTRKRTETR 180

Query: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLEGDEKMEAITVHMPFDDMPKISEPMKENMELE 240
           NNGAAEQRVYSTRRSVRLLEKNMESLSL GDE+ME I+VHM F+DMP  S P+KEN ELE
Sbjct: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLGGDEEMEPISVHMSFEDMPNSSVPVKENTELE 240

Query: 241 TESKKTDESKSKLDENLSVEVDDGEKNEMGSEVKLSDEEPEMVLDDLLKSFDENVISAKS 300
            ESK  DES+SKLDE+L VEVDDGEK+E+GSEVKLS+EEPE+VLDDLLKS DENV++AKS
Sbjct: 241 IESKIIDESESKLDEDLRVEVDDGEKSEIGSEVKLSEEEPEIVLDDLLKSSDENVVTAKS 300

Query: 301 VDDSKIEAAHANSDVKCFSEDEKVNEVSKDDDFVEISAEEINESDKSSLPTEDLDVTE-V 360
           VDDS+ EA HAN DVKCFSED KVNE SK    VEISAEEINESDKSSLPTEDLDVTE V
Sbjct: 301 VDDSETEALHANLDVKCFSED-KVNEASK---AVEISAEEINESDKSSLPTEDLDVTEVV 360

Query: 361 HKSSIAEEFGMEFESQQNESTCELE 385
           HKS+IAEEFGME E +QNEST ELE
Sbjct: 361 HKSNIAEEFGMEVECKQNESTSELE 381

BLAST of CSPI05G01660 vs. TAIR 10
Match: AT3G03130.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G17160.1); Has 7130 Blast hits to 3690 proteins in 504 species: Archae - 22; Bacteria - 2666; Metazoa - 1654; Fungi - 721; Plants - 246; Viruses - 56; Other Eukaryotes - 1765 (source: NCBI BLink). )

HSP 1 Score: 98.6 bits (244), Expect = 1.2e-20
Identity = 126/397 (31.74%), Postives = 195/397 (49.12%), Query Frame = 0

Query: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKA 60
           MDFH+L RR+LQ  CKRNKIPAN+TN+AMADAL  L+ VEG++EF++  R   P S    
Sbjct: 1   MDFHSLPRRDLQFFCKRNKIPANMTNIAMADALRDLEIVEGMDEFMDPSRDQSPTS---- 60

Query: 61  EVISSEIP---RIALRTSTRRKAVKDET----ITTRS---RQGAVARDMEESEDKDLNMA 120
             ++  +P   R A RT TRRK+ KDET    + TRS      ++A +M++ E+KD+NM 
Sbjct: 61  --VARNLPSAARTAART-TRRKSTKDETQSSELVTRSCYVVSKSLAGEMDQ-ENKDMNM- 120

Query: 121 LTTPSLPGSWRRTAAASSAC*KFDFQMTVDDQKEDKDMDQEKKEIENTPAVPKSQKRVAG 180
           L  PS+P S                       K D +    +  +  TPA   +++  A 
Sbjct: 121 LQNPSVPQS--------------------RAVKLDVNDIMPEANVSKTPAARSTRRAQAA 180

Query: 181 ASTRKRTETKNNGAAEQRVYSTRRSVRLLEKNMESLSLEGDEKMEAITVHMPFDDMPKIS 240
           AS++K         + QRVYSTRRSVRLLE++M  LSL+ +  ++        +D P  S
Sbjct: 181 ASSKK-------DESVQRVYSTRRSVRLLEESMADLSLKTNVPVK------KHEDSPAGS 240

Query: 241 EPMKENMELETESKKTDES--KSKLDENLSVEVD-DGEKNEMGSEVKLSDEEPEMVLDDL 300
              K   + +  S+ TD+    S  D N S+E + DG KN+   ++   D      L D+
Sbjct: 241 ---KFQAKSDENSENTDKGGVMSGRDLNDSLEKEWDGSKNDPDLDILYGD------LGDI 300

Query: 301 LKSFDENVISAKSVDDSKIEAAHANSDVKCFSEDEKVNEVSKDDDFVEISAEEINESDKS 360
             +F +   S + ++ +      A+      +E    +E S++D FV +           
Sbjct: 301 --TFFDASTSKEHLNRTDSSTVSASDSFVLVNE----HETSQEDGFVVVD---------H 331

Query: 361 SLPTEDLDVTEVHKSSIAEEFGMEFESQQNESTCELE 385
           +  T   +    +K S  E+  ++ ES+  E+  E +
Sbjct: 361 ATSTTTTNTLACNKESEPEQMKIDSESESEETEYETD 331

BLAST of CSPI05G01660 vs. TAIR 10
Match: AT5G17160.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G03130.1); Has 14330 Blast hits to 10381 proteins in 896 species: Archae - 94; Bacteria - 2881; Metazoa - 4019; Fungi - 1576; Plants - 515; Viruses - 110; Other Eukaryotes - 5135 (source: NCBI BLink). )

HSP 1 Score: 92.0 bits (227), Expect = 1.1e-18
Identity = 105/372 (28.23%), Postives = 172/372 (46.24%), Query Frame = 0

Query: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKA 60
           MDFH+L RR+LQ LCKRNKIPAN+TN+AMADAL +L+ V+G++E++N   S  P SP   
Sbjct: 1   MDFHSLLRRDLQFLCKRNKIPANMTNIAMADALKSLEIVDGLDEYMNQSESSAPHSP--- 60

Query: 61  EVISSEIPRIALRTSTRRKAVKDETITTRSRQGAVARDMEESEDKDLNMALTTPSLPGSW 120
               + + ++   T+TR         TTR +    A     S+    +   T+ SL G  
Sbjct: 61  ----TSVAKLPPSTATR---------TTRRKTTTKAEPQPSSQLVSRSCRSTSKSLAGD- 120

Query: 121 RRTAAASSAC*KFDFQMTVDDQKEDKDMDQEKK--------EIENTPAVPKSQKRVAGAS 180
                             +D +  +K++ QE K         +  TPA   ++K  A  S
Sbjct: 121 ------------------MDQENINKNVAQEMKTSNVKFEANVLKTPAAGSTRKTSAATS 180

Query: 181 TRKRTETKNNGAAEQRVYSTRRSVRLLEKNMESLSLEGDEKMEAITVHMPFDDMPKISEP 240
             K+ E        Q VYSTRRS RLLEK M  LSL+  E +         D+ P  +E 
Sbjct: 181 CTKKDE------LVQSVYSTRRSTRLLEKCMADLSLKTKETV---------DNKPAKNED 240

Query: 241 MKENMELETESKKTDESKSKLDENLSVEVDDGEKNEMGSEVKLSDEEPEMVLDDLLKSFD 300
            ++ +  + ++    E +     +LSV ++   +N      K++ +   +V+ D     +
Sbjct: 241 TEQKVSAQEKNLTGSEGEVIPGRDLSVSMEQVWENLKNDSDKIAGDLEVIVVMDANTEAN 300

Query: 301 ENVISAKSVDDSKIEAAHANSDVKCFSEDEKVNEVSKDDDFVEISAEEINE----SDKSS 360
           +  ++  + D  + E    NS V+   E+E +  + ++      + +EI +     D S 
Sbjct: 301 KEEMNEVTADKKESE----NSLVQVDKEEETLQAICEEGPKKNDNDQEIGDLVIYVDVSD 318

BLAST of CSPI05G01660 vs. TAIR 10
Match: AT1G76740.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G76840.1); Has 3683 Blast hits to 2942 proteins in 407 species: Archae - 9; Bacteria - 440; Metazoa - 1472; Fungi - 269; Plants - 192; Viruses - 8; Other Eukaryotes - 1293 (source: NCBI BLink). )

HSP 1 Score: 48.5 bits (114), Expect = 1.4e-05
Identity = 21/40 (52.50%), Postives = 30/40 (75.00%), Query Frame = 0

Query: 1  MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVE 41
          M+FH + R++LQALCK+N IPAN+ N+ MA  LA++   E
Sbjct: 1  MNFHGMKRKDLQALCKKNGIPANLKNIEMATRLASVLQKE 40