CSPI04G20620 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G20620
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Cytosolic Fe-S cluster assembly factor NBP35
Location	Chr4: 18845288 .. 18846485 (-)
RNA-Seq Expression	CSPI04G20620
Synteny	CSPI04G20620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCAGACCTGATTTCAATAGCGGAACGAATGGCAACGGTTAAGCACAAGATTCTTGTTCTATCAGGTAAGGGTGGGGTCGGAAAGAGCACATTCTCTGCTCAGCTCGCATTTGCCCTAGCTGCCATGGACTTCCAGGTGGGTCTCCTTGATATTGATATTTGTGGCCCGAGTATCCCGAAGATGCTTGGTCTAGAAGGTCAAGATGTGCATCAGAGTAACCTTGGATGGTCCCCTGTTTACATAGAGTCCAACCTTGGAGTTATGTCGATTGGTTTCATGCTCCCTAACCCTGACGAAGCGGTCATTTGGAGAGGCCCTCGAAAAAATGGGCTTATTAAGCAGTTTCTAAAGGATGTGTATTGGGGAGAGCTCGATTTTCTGGTTGTTGATGCTCCACCAGGGACTTCAGATGAGCACATATCAATTGTACAATGCCTTAAGGCTACTGGAATTGACGGTGCAATCATTGTCACAACTCCACAACAAGTATCTCTCATCGATGTGCGGAAAGAAGTGAGTTTCTGCAAAACGGTAGGAGTAAAAGTTCTTGGAGTTGTAGAGAACATGAGCAGCTTGTGCCAACCACTATTAGATATGAAGTTTTTGAAGACAACAGAGGCCGGAGAGCAGACAGATGTGACAGAATGGGTGGGAGAGTACATTCGAGAAAAAGCGCCGGAGCTTCTAAACTTGATTACTTATAGTGAAGTGTTCGATAGTAGTGGAGGTGGTGCTGCAAAAATGTGTAGGGAGATGGATGTGCCATTCCTAGGGAAGGTACCATTGGATCCACAGCTTTGCAAGGCAGCTGAAGAAGGAAGGTCCTGTTTTGGTGACCAAAAATGTGGGGTAAGTGCCTCAGCACTGAAGAAGATTATAGAGAAGTTGATACAAAATCAAGGGTTTTCAACAATGTCAATAGATGCTAAAGCCTAGGACAGACAGCACAGCTTTTGAACAAATGTGGTTGTGCAATTACTTAATACAGCACAACTTTTGAACACATCTTCCTTTTCTCTTTGGTCTTAGTTTGTAAGGTATCATAATGTACTTCACTTTTATTACTTCTTCACAAAAGGTATATGTTTGTTTCCTTTGGTGGATTTATAGTTATCTCATTCCTATAGCTTAGTCAATATTCCTGCACATCAATTTGTTAAAACTCGAAAGAAGAATGTTAGGGGATAATAATTTTTTT

mRNA sequence

CCAGACCTGATTTCAATAGCGGAACGAATGGCAACGGTTAAGCACAAGATTCTTGTTCTATCAGGTAAGGGTGGGGTCGGAAAGAGCACATTCTCTGCTCAGCTCGCATTTGCCCTAGCTGCCATGGACTTCCAGGTGGGTCTCCTTGATATTGATATTTGTGGCCCGAGTATCCCGAAGATGCTTGGTCTAGAAGGTCAAGATGTGCATCAGAGTAACCTTGGATGGTCCCCTGTTTACATAGAGTCCAACCTTGGAGTTATGTCGATTGGTTTCATGCTCCCTAACCCTGACGAAGCGGTCATTTGGAGAGGCCCTCGAAAAAATGGGCTTATTAAGCAGTTTCTAAAGGATGTGTATTGGGGAGAGCTCGATTTTCTGGTTGTTGATGCTCCACCAGGGACTTCAGATGAGCACATATCAATTGTACAATGCCTTAAGGCTACTGGAATTGACGGTGCAATCATTGTCACAACTCCACAACAAGTATCTCTCATCGATGTGCGGAAAGAAGTGAGTTTCTGCAAAACGGTAGGAGTAAAAGTTCTTGGAGTTGTAGAGAACATGAGCAGCTTGTGCCAACCACTATTAGATATGAAGTTTTTGAAGACAACAGAGGCCGGAGAGCAGACAGATGTGACAGAATGGGTGGGAGAGTACATTCGAGAAAAAGCGCCGGAGCTTCTAAACTTGATTACTTATAGTGAAGTGTTCGATAGTAGTGGAGGTGGTGCTGCAAAAATGTGTAGGGAGATGGATGTGCCATTCCTAGGGAAGGTACCATTGGATCCACAGCTTTGCAAGGCAGCTGAAGAAGGAAGGTCCTGTTTTGGTGACCAAAAATGTGGGGTAAGTGCCTCAGCACTGAAGAAGATTATAGAGAAGTTGATACAAAATCAAGGGTTTTCAACAATGTCAATAGATGCTAAAGCCTAGGACAGACAGCACAGCTTTTGAACAAATGTGGTTGTGCAATTACTTAATACAGCACAACTTTTGAACACATCTTCCTTTTCTCTTTGGTCTTAGTTTGTAAGGTATCATAATGTACTTCACTTTTATTACTTCTTCACAAAAGGTATATGTTTGTTTCCTTTGGTGGATTTATAGTTATCTCATTCCTATAGCTTAGTCAATATTCCTGCACATCAATTTGTTAAAACTCGAAAGAAGAATGTTAGGGGATAATAATTTTTTT

Coding sequence (CDS)

ATGGCAACGGTTAAGCACAAGATTCTTGTTCTATCAGGTAAGGGTGGGGTCGGAAAGAGCACATTCTCTGCTCAGCTCGCATTTGCCCTAGCTGCCATGGACTTCCAGGTGGGTCTCCTTGATATTGATATTTGTGGCCCGAGTATCCCGAAGATGCTTGGTCTAGAAGGTCAAGATGTGCATCAGAGTAACCTTGGATGGTCCCCTGTTTACATAGAGTCCAACCTTGGAGTTATGTCGATTGGTTTCATGCTCCCTAACCCTGACGAAGCGGTCATTTGGAGAGGCCCTCGAAAAAATGGGCTTATTAAGCAGTTTCTAAAGGATGTGTATTGGGGAGAGCTCGATTTTCTGGTTGTTGATGCTCCACCAGGGACTTCAGATGAGCACATATCAATTGTACAATGCCTTAAGGCTACTGGAATTGACGGTGCAATCATTGTCACAACTCCACAACAAGTATCTCTCATCGATGTGCGGAAAGAAGTGAGTTTCTGCAAAACGGTAGGAGTAAAAGTTCTTGGAGTTGTAGAGAACATGAGCAGCTTGTGCCAACCACTATTAGATATGAAGTTTTTGAAGACAACAGAGGCCGGAGAGCAGACAGATGTGACAGAATGGGTGGGAGAGTACATTCGAGAAAAAGCGCCGGAGCTTCTAAACTTGATTACTTATAGTGAAGTGTTCGATAGTAGTGGAGGTGGTGCTGCAAAAATGTGTAGGGAGATGGATGTGCCATTCCTAGGGAAGGTACCATTGGATCCACAGCTTTGCAAGGCAGCTGAAGAAGGAAGGTCCTGTTTTGGTGACCAAAAATGTGGGGTAAGTGCCTCAGCACTGAAGAAGATTATAGAGAAGTTGATACAAAATCAAGGGTTTTCAACAATGTCAATAGATGCTAAAGCCTAG

Protein sequence

MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENMSSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMCREMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDAKA*

Homology

[Close]

BLAST of CSPI04G20620 vs. ExPASy Swiss-Prot
Match: Q8H1Q2 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Arabidopsis thaliana OX=3702 GN=NBP35 PE=1 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 6.6e-133
Identity = 232/289 (80.28%), Postives = 257/289 (88.93%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 60
           M+TVKHKILVLSGKGGVGKSTFSAQL+FALA MD QVGL+DIDICGPSIPKMLGLEGQ++
Sbjct: 55  MSTVKHKILVLSGKGGVGKSTFSAQLSFALAGMDHQVGLMDIDICGPSIPKMLGLEGQEI 114

Query: 61  HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 120
           HQSNLGWSPVY+E NLGVMSIGFMLPN DEAVIWRGPRKNGLIKQFLKDVYWGE+D+LVV
Sbjct: 115 HQSNLGWSPVYVEDNLGVMSIGFMLPNSDEAVIWRGPRKNGLIKQFLKDVYWGEIDYLVV 174

Query: 121 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 180
           DAPPGTSDEHISIVQ L  TGIDGAIIVTTPQ+VSLIDVRKEVSFCK VGV VLGVVENM
Sbjct: 175 DAPPGTSDEHISIVQYLLPTGIDGAIIVTTPQEVSLIDVRKEVSFCKKVGVPVLGVVENM 234

Query: 181 SSLCQPLLDMKFLK-TTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKM 240
           S L QPL D+KF+K  TE G   +VTE V   +R+ APELL+++  SEVFDSSGGGA +M
Sbjct: 235 SGLSQPLKDVKFMKLATETGSSINVTEDVIACLRKNAPELLDIVACSEVFDSSGGGAERM 294

Query: 241 CREMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLI 289
           CREM VPFLGKVP+DPQLCKAAE+G+SCF D KC +SA ALK II+K++
Sbjct: 295 CREMGVPFLGKVPMDPQLCKAAEQGKSCFEDNKCLISAPALKSIIQKVV 343

[Close]

BLAST of CSPI04G20620 vs. ExPASy Swiss-Prot
Match: A7RUD5 (Cytosolic Fe-S cluster assembly factor NUBP1 homolog OS=Nematostella vectensis OX=45351 GN=v1g236650 PE=3 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 2.1e-86
Identity = 161/290 (55.52%), Postives = 202/290 (69.66%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAA-MDFQVGLLDIDICGPSIPKMLGLEGQD 60
           +++VKHKILVLSGKGGVGKSTF+A LA  LAA  D QV +LDIDICGPSIP + GL+G+ 
Sbjct: 54  LSSVKHKILVLSGKGGVGKSTFTAHLAHGLAADEDRQVAVLDIDICGPSIPTVFGLQGEQ 113

Query: 61  VHQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLV 120
           VHQS  GWSPVY+E NLGVMS+GF+L  P +AVIWRGP+KNGLIKQFL+DV WG+ DFLV
Sbjct: 114 VHQSGSGWSPVYVEDNLGVMSVGFLLAKPTDAVIWRGPKKNGLIKQFLRDVDWGDADFLV 173

Query: 121 VDAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVEN 180
           VD PPGTSDEH+SI+Q L  T +DGA++VTTPQ+VSL+DVRKE+SFCK V + V+GVVEN
Sbjct: 174 VDTPPGTSDEHLSIIQYLNQTEVDGAVVVTTPQEVSLLDVRKEISFCKKVRLPVIGVVEN 233

Query: 181 MSSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKM 240
           MS    P                                  N    S++F  + GGA KM
Sbjct: 234 MSVFVCP----------------------------------NCKKESQIFPPTTGGAEKM 293

Query: 241 CREMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQ 290
             EM VPFLG++PLDP++ +A +EG+S   +     + S+ K IIEK+++
Sbjct: 294 AVEMKVPFLGRIPLDPRIGRACDEGKSFLSEIPDSPATSSYKDIIEKIVK 309

[Close]

BLAST of CSPI04G20620 vs. ExPASy Swiss-Prot
Match: Q6P298 (Cytosolic Fe-S cluster assembly factor nubp1 OS=Danio rerio OX=7955 GN=nubp1 PE=2 SV=2)

HSP 1 Score: 318.9 bits (816), Expect = 6.1e-86
Identity = 157/288 (54.51%), Postives = 204/288 (70.83%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAA-MDFQVGLLDIDICGPSIPKMLGLEGQD 60
           M +VKHKILVLSGKGGVGKSTFSA L+ ALA+    +V LLD+DICGPSIPK++GLEG+ 
Sbjct: 54  MTSVKHKILVLSGKGGVGKSTFSAHLSHALASDSSKEVALLDVDICGPSIPKIMGLEGEQ 113

Query: 61  VHQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLV 120
           VHQS  GWSPVY+E NL VMSIGF+L +PD+AVIWRGP+KNG+IKQFL+DV WGE+D+L+
Sbjct: 114 VHQSGSGWSPVYVEDNLAVMSIGFLLSSPDDAVIWRGPKKNGMIKQFLRDVDWGEVDYLI 173

Query: 121 VDAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVEN 180
           VD PPGTSDEH+SIVQ L   GIDGA+I+TTPQ+VSL DVRKE+ FCK V + +LGV+EN
Sbjct: 174 VDTPPGTSDEHLSIVQYLSGAGIDGAVIITTPQEVSLQDVRKEIRFCKKVNLPILGVIEN 233

Query: 181 MSSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKM 240
           MS    P       K T                             S++F  + GGA +M
Sbjct: 234 MSGFVCPK-----CKNT-----------------------------SQIFPPTTGGAQRM 293

Query: 241 CREMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKL 288
           C E+++P LG++PLDP++ K+ +EG+S   +     +A+A + I++K+
Sbjct: 294 CEELNLPLLGRIPLDPRIGKSCDEGKSFLTEVPDSPAAAAYQSIVQKI 307

[Close]

BLAST of CSPI04G20620 vs. ExPASy Swiss-Prot
Match: Q5I0L4 (Cytosolic Fe-S cluster assembly factor NUBP1 OS=Rattus norvegicus OX=10116 GN=Nubp1 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 4.3e-84
Identity = 155/288 (53.82%), Postives = 200/288 (69.44%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAA-MDFQVGLLDIDICGPSIPKMLGLEGQD 60
           M TV+H+ILVLSGKGGVGKSTFSA LA  LA   D QV LLDIDICGPSIPK++GLEG+ 
Sbjct: 50  MKTVRHRILVLSGKGGVGKSTFSAHLAHGLAEDGDTQVALLDIDICGPSIPKIMGLEGEQ 109

Query: 61  VHQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLV 120
           VHQS  GWSPVY+E NLGVMS+GF+L +PD+AVIWRGP+KNG+IKQFL+DV WG++D+LV
Sbjct: 110 VHQSGSGWSPVYVEDNLGVMSVGFLLSSPDDAVIWRGPKKNGMIKQFLRDVDWGDVDYLV 169

Query: 121 VDAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVEN 180
           +D PPGTSDEH+S+VQ L A  IDGA+I+TTPQ+V+L DVRKE+SFC  V + ++GVVEN
Sbjct: 170 IDTPPGTSDEHLSVVQYLAAAHIDGAVILTTPQEVALQDVRKEISFCHKVKLPIIGVVEN 229

Query: 181 MSSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKM 240
           MS    P    +                                  S++F  + GGA  M
Sbjct: 230 MSGFICPKCKRE----------------------------------SQIFPPTTGGAEAM 289

Query: 241 CREMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKL 288
           C+ + +P LGKVPLDP + K+ ++G+S F +     + +A K II+++
Sbjct: 290 CQALKIPLLGKVPLDPHIGKSCDKGQSFFVEAPDSPATAAYKSIIQRI 303

[Close]

BLAST of CSPI04G20620 vs. ExPASy Swiss-Prot
Match: Q3KQF0 (Cytosolic Fe-S cluster assembly factor nubp1-A OS=Xenopus laevis OX=8355 GN=nubp1-A PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 5.7e-84
Identity = 154/288 (53.47%), Postives = 200/288 (69.44%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMD-FQVGLLDIDICGPSIPKMLGLEGQD 60
           M+ VKHKILVLSGKGGVGKSTFSA LA  LA  +  +V LLD+DICGPSIPKM+GLEG+ 
Sbjct: 54  MSLVKHKILVLSGKGGVGKSTFSAHLAHGLAQDEGKEVALLDVDICGPSIPKMMGLEGEQ 113

Query: 61  VHQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLV 120
           VHQS  GWSPVY+E NL VMS+GF+L +PD+AVIWRGP+KNG+IKQFL+DV WG++D+L+
Sbjct: 114 VHQSGSGWSPVYVEDNLAVMSVGFLLSSPDDAVIWRGPKKNGMIKQFLRDVDWGDVDYLI 173

Query: 121 VDAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVEN 180
           VD PPGTSDEH+S+VQ L A GIDGA+I+TTPQ+VSL DVRKE++FC+ V + ++GVVEN
Sbjct: 174 VDTPPGTSDEHLSVVQYLSAAGIDGAVIITTPQEVSLQDVRKEINFCRKVKLPIIGVVEN 233

Query: 181 MSSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKM 240
           MS    P    +                                  S++F  + GGA KM
Sbjct: 234 MSGFICPKCKNE----------------------------------SQIFPPTTGGAEKM 293

Query: 241 CREMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKL 288
           C ++ V  LGKVPLDP + K+ + G+S F +     +  + +KII+++
Sbjct: 294 CTDLSVSLLGKVPLDPNIGKSCDTGKSFFTEIPDSPATLSYRKIIQRI 307

[Close]

BLAST of CSPI04G20620 vs. ExPASy TrEMBL
Match: A0A0A0L2L1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G540120 PE=3 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 1.3e-171
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 60
           MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV
Sbjct: 21  MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 80

Query: 61  HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 120
           HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV
Sbjct: 81  HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 140

Query: 121 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 180
           DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM
Sbjct: 141 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 200

Query: 181 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 240
           SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC
Sbjct: 201 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 260

Query: 241 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 300
           REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA
Sbjct: 261 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 320

Query: 301 KA 303
           KA
Sbjct: 321 KA 322

[Close]

BLAST of CSPI04G20620 vs. ExPASy TrEMBL
Match: A0A1S3B5W7 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucumis melo OX=3656 GN=LOC103486120 PE=3 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 3.7e-171
Identity = 301/302 (99.67%), Postives = 302/302 (100.00%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 60
           MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV
Sbjct: 55  MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 114

Query: 61  HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 120
           HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV
Sbjct: 115 HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 174

Query: 121 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 180
           DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGV+VLGVVENM
Sbjct: 175 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVEVLGVVENM 234

Query: 181 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 240
           SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC
Sbjct: 235 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 294

Query: 241 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 300
           REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA
Sbjct: 295 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 354

Query: 301 KA 303
           KA
Sbjct: 355 KA 356

[Close]

BLAST of CSPI04G20620 vs. ExPASy TrEMBL
Match: A0A5D3D0N0 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold32G00110 PE=3 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 3.7e-171
Identity = 301/302 (99.67%), Postives = 302/302 (100.00%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 60
           MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV
Sbjct: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 60

Query: 61  HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 120
           HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV
Sbjct: 61  HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 120

Query: 121 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 180
           DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGV+VLGVVENM
Sbjct: 121 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVEVLGVVENM 180

Query: 181 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 240
           SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC
Sbjct: 181 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 240

Query: 241 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 300
           REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA
Sbjct: 241 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 300

Query: 301 KA 303
           KA
Sbjct: 301 KA 302

[Close]

BLAST of CSPI04G20620 vs. ExPASy TrEMBL
Match: A0A6J1JQ09 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucurbita maxima OX=3661 GN=LOC111486691 PE=3 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 3.6e-158
Identity = 279/302 (92.38%), Postives = 287/302 (95.03%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 60
           MATVKHKILVLSGKGGVGKSTFSAQL+FALAAMDFQVGL+DIDICGPSIPKMLGLEGQDV
Sbjct: 55  MATVKHKILVLSGKGGVGKSTFSAQLSFALAAMDFQVGLMDIDICGPSIPKMLGLEGQDV 114

Query: 61  HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 120
           HQSNLGWSPVYIESNLGVMSIGFMLPNPD+AVIWRGPRKNGLIKQFLKDVYWGELDFL+V
Sbjct: 115 HQSNLGWSPVYIESNLGVMSIGFMLPNPDDAVIWRGPRKNGLIKQFLKDVYWGELDFLIV 174

Query: 121 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 180
           DAPPGTSDEHISIVQCL+ATGIDGAIIVTTPQQVSLIDVRKEVSFCK VGVKVLGVVENM
Sbjct: 175 DAPPGTSDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENM 234

Query: 181 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 240
           S L QPLLD+KFLK TE  EQ DVTEW  EYIREKAPELLNLIT SEVFDSSGGGAAKMC
Sbjct: 235 SGLTQPLLDLKFLKATETEEQVDVTEWAAEYIREKAPELLNLITCSEVFDSSGGGAAKMC 294

Query: 241 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 300
           REM VPFLGKVPLDPQLCKAAEEGRSCFGDQKC VSA ALKKIIE LI++QGFSTMSID 
Sbjct: 295 REMGVPFLGKVPLDPQLCKAAEEGRSCFGDQKCRVSAPALKKIIENLIESQGFSTMSIDD 354

Query: 301 KA 303
           KA
Sbjct: 355 KA 356

[Close]

BLAST of CSPI04G20620 vs. ExPASy TrEMBL
Match: A0A6J1ERE1 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucurbita moschata OX=3662 GN=LOC111435180 PE=3 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 3.6e-158
Identity = 278/302 (92.05%), Postives = 288/302 (95.36%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 60
           MATVKHKILVLSGKGGVGKSTFSAQL+FALAAMDFQVGL+DIDICGPSIPKMLGLEGQDV
Sbjct: 55  MATVKHKILVLSGKGGVGKSTFSAQLSFALAAMDFQVGLMDIDICGPSIPKMLGLEGQDV 114

Query: 61  HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 120
           HQSNLGWSPVYIESNLGVMSIGFMLPNPD+AVIWRGPRKNGLIKQFLKDVYWGELDFL+V
Sbjct: 115 HQSNLGWSPVYIESNLGVMSIGFMLPNPDDAVIWRGPRKNGLIKQFLKDVYWGELDFLIV 174

Query: 121 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 180
           DAPPGTSDEHISIVQCL+ATGIDGAIIVTTPQQVSLIDVRKEVSFCK VGVKVLGVVENM
Sbjct: 175 DAPPGTSDEHISIVQCLEATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENM 234

Query: 181 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 240
           S L QPLLD+KFLKTTE  EQ DVTEW  EYIREKAPELLNLIT SEVFDSSGGGAAKMC
Sbjct: 235 SGLTQPLLDLKFLKTTETEEQVDVTEWAAEYIREKAPELLNLITCSEVFDSSGGGAAKMC 294

Query: 241 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 300
           R+M VPFLGKVPLDPQLCKAAEEGRSCFGDQKC VSA ALKKIIE L+++QGFSTMSID 
Sbjct: 295 RDMGVPFLGKVPLDPQLCKAAEEGRSCFGDQKCRVSAPALKKIIENLLESQGFSTMSIDD 354

Query: 301 KA 303
           KA
Sbjct: 355 KA 356

[Close]

BLAST of CSPI04G20620 vs. NCBI nr
Match: KAE8649747.1 (hypothetical protein Csa_012684 [Cucumis sativus])

HSP 1 Score: 612.1 bits (1577), Expect = 2.6e-171
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 60
           MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV
Sbjct: 79  MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 138

Query: 61  HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 120
           HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV
Sbjct: 139 HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 198

Query: 121 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 180
           DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM
Sbjct: 199 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 258

Query: 181 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 240
           SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC
Sbjct: 259 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 318

Query: 241 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 300
           REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA
Sbjct: 319 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 378

Query: 301 KA 303
           KA
Sbjct: 379 KA 380

[Close]

BLAST of CSPI04G20620 vs. NCBI nr
Match: XP_004144673.1 (cytosolic Fe-S cluster assembly factor NBP35 [Cucumis sativus] >XP_011653919.1 cytosolic Fe-S cluster assembly factor NBP35 [Cucumis sativus] >XP_031739512.1 cytosolic Fe-S cluster assembly factor NBP35 [Cucumis sativus] >XP_031739513.1 cytosolic Fe-S cluster assembly factor NBP35 [Cucumis sativus] >XP_031739514.1 cytosolic Fe-S cluster assembly factor NBP35 [Cucumis sativus])

HSP 1 Score: 612.1 bits (1577), Expect = 2.6e-171
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 60
           MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV
Sbjct: 55  MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 114

Query: 61  HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 120
           HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV
Sbjct: 115 HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 174

Query: 121 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 180
           DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM
Sbjct: 175 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 234

Query: 181 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 240
           SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC
Sbjct: 235 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 294

Query: 241 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 300
           REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA
Sbjct: 295 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 354

Query: 301 KA 303
           KA
Sbjct: 355 KA 356

[Close]

BLAST of CSPI04G20620 vs. NCBI nr
Match: XP_008442190.1 (PREDICTED: cytosolic Fe-S cluster assembly factor NBP35 [Cucumis melo] >XP_008442191.1 PREDICTED: cytosolic Fe-S cluster assembly factor NBP35 [Cucumis melo] >XP_008442192.1 PREDICTED: cytosolic Fe-S cluster assembly factor NBP35 [Cucumis melo])

HSP 1 Score: 610.5 bits (1573), Expect = 7.7e-171
Identity = 301/302 (99.67%), Postives = 302/302 (100.00%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 60
           MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV
Sbjct: 55  MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 114

Query: 61  HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 120
           HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV
Sbjct: 115 HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 174

Query: 121 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 180
           DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGV+VLGVVENM
Sbjct: 175 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVEVLGVVENM 234

Query: 181 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 240
           SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC
Sbjct: 235 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 294

Query: 241 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 300
           REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA
Sbjct: 295 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 354

Query: 301 KA 303
           KA
Sbjct: 355 KA 356

[Close]

BLAST of CSPI04G20620 vs. NCBI nr
Match: KAA0041216.1 (cytosolic Fe-S cluster assembly factor NBP35 [Cucumis melo var. makuwa] >TYK16019.1 cytosolic Fe-S cluster assembly factor NBP35 [Cucumis melo var. makuwa])

HSP 1 Score: 610.5 bits (1573), Expect = 7.7e-171
Identity = 301/302 (99.67%), Postives = 302/302 (100.00%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 60
           MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV
Sbjct: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 60

Query: 61  HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 120
           HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV
Sbjct: 61  HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 120

Query: 121 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 180
           DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGV+VLGVVENM
Sbjct: 121 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVEVLGVVENM 180

Query: 181 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 240
           SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC
Sbjct: 181 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 240

Query: 241 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 300
           REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA
Sbjct: 241 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 300

Query: 301 KA 303
           KA
Sbjct: 301 KA 302

[Close]

BLAST of CSPI04G20620 vs. NCBI nr
Match: XP_038883029.1 (cytosolic Fe-S cluster assembly factor NBP35 [Benincasa hispida])

HSP 1 Score: 594.0 bits (1530), Expect = 7.5e-166
Identity = 293/302 (97.02%), Postives = 297/302 (98.34%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 60
           MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV
Sbjct: 55  MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 114

Query: 61  HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 120
           HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV
Sbjct: 115 HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 174

Query: 121 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 180
           DAPPGTSDEHISIVQCL+A+GIDGAIIVTTPQQVSLIDVRKEVSFCK VGVKVLGVVENM
Sbjct: 175 DAPPGTSDEHISIVQCLEASGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENM 234

Query: 181 SSLCQPLLDMKFLKTTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMC 240
           SSLCQPLLDMKFLKTTEAGEQTDVTEWV EYIREKAPELLN+I+ SEVFDSSGGGAA MC
Sbjct: 235 SSLCQPLLDMKFLKTTEAGEQTDVTEWVAEYIREKAPELLNVISCSEVFDSSGGGAANMC 294

Query: 241 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDA 300
           REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSID 
Sbjct: 295 REMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMSIDD 354

Query: 301 KA 303
           KA
Sbjct: 355 KA 356

[Close]

BLAST of CSPI04G20620 vs. TAIR 10
Match: AT5G50960.1 (nucleotide binding protein 35 )

HSP 1 Score: 474.9 bits (1221), Expect = 4.7e-134
Identity = 232/289 (80.28%), Postives = 257/289 (88.93%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 60
           M+TVKHKILVLSGKGGVGKSTFSAQL+FALA MD QVGL+DIDICGPSIPKMLGLEGQ++
Sbjct: 55  MSTVKHKILVLSGKGGVGKSTFSAQLSFALAGMDHQVGLMDIDICGPSIPKMLGLEGQEI 114

Query: 61  HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 120
           HQSNLGWSPVY+E NLGVMSIGFMLPN DEAVIWRGPRKNGLIKQFLKDVYWGE+D+LVV
Sbjct: 115 HQSNLGWSPVYVEDNLGVMSIGFMLPNSDEAVIWRGPRKNGLIKQFLKDVYWGEIDYLVV 174

Query: 121 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 180
           DAPPGTSDEHISIVQ L  TGIDGAIIVTTPQ+VSLIDVRKEVSFCK VGV VLGVVENM
Sbjct: 175 DAPPGTSDEHISIVQYLLPTGIDGAIIVTTPQEVSLIDVRKEVSFCKKVGVPVLGVVENM 234

Query: 181 SSLCQPLLDMKFLK-TTEAGEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKM 240
           S L QPL D+KF+K  TE G   +VTE V   +R+ APELL+++  SEVFDSSGGGA +M
Sbjct: 235 SGLSQPLKDVKFMKLATETGSSINVTEDVIACLRKNAPELLDIVACSEVFDSSGGGAERM 294

Query: 241 CREMDVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSASALKKIIEKLI 289
           CREM VPFLGKVP+DPQLCKAAE+G+SCF D KC +SA ALK II+K++
Sbjct: 295 CREMGVPFLGKVPMDPQLCKAAEQGKSCFEDNKCLISAPALKSIIQKVV 343

[Close]

BLAST of CSPI04G20620 vs. TAIR 10
Match: AT4G19540.1 (IND1(iron-sulfur protein required for NADH dehydrogenase)-like )

HSP 1 Score: 141.7 bits (356), Expect = 9.4e-34
Identity = 87/203 (42.86%), Postives = 118/203 (58.13%), Query Frame = 0


Query: 4   VKHKILVLSGKGGVGKSTFSAQLAFALA-AMDFQVGLLDIDICGPSIPKMLGLEGQDVHQ 63
           VK  I V SGKGGVGKS+ +  LA ALA   + ++GLLD D+ GPS+P M+ +  +    
Sbjct: 42  VKDIIAVASGKGGVGKSSTAVNLAVALANKCELKIGLLDADVYGPSVPIMMNINQKPQVN 101

Query: 64  SNLGWSPVYIESNLGV--MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 123
            ++   PV    N GV  MS+G +L   D  ++WRGP     + +  K V WG+LD LVV
Sbjct: 102 QDMKMIPV---ENYGVKCMSMG-LLVEKDAPLVWRGPMVMSALAKMTKGVDWGDLDILVV 161

Query: 124 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 183
           D PPGT D  ISI Q LK   + GA+IV+TPQ V+L D  + +S    V V +LG+VENM
Sbjct: 162 DMPPGTGDAQISISQNLK---LSGAVIVSTPQDVALADANRGISMFDKVRVPILGLVENM 221

Query: 184 SS-LCQPLLDMKFLKTTEAGEQT 203
           S  +C    +  F+   E   +T
Sbjct: 222 SCFVCPHCNEPSFIFGKEGARRT 237

[Close]

BLAST of CSPI04G20620 vs. TAIR 10
Match: AT3G24430.1 (ATP binding )

HSP 1 Score: 127.1 bits (318), Expect = 2.4e-29
Identity = 71/180 (39.44%), Postives = 103/180 (57.22%), Query Frame = 0


Query: 1   MATVKHKILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 60
           ++ + + I V S KGGVGKST +  LA+ LA M  +VG+ D D+ GPS+P M+  E + +
Sbjct: 172 LSRISNIIAVSSCKGGVGKSTVAVNLAYTLAGMGARVGIFDADVYGPSLPTMVNPESR-I 231

Query: 61  HQSNLGWSPVYIESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 120
            + N     +     +GV  + F       A++ RGP  +G+I Q L    WGELD+LV+
Sbjct: 232 LEMNPEKKTIIPTEYMGVKLVSFGFAGQGRAIM-RGPMVSGVINQLLTTTEWGELDYLVI 291

Query: 121 DAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVVENM 180
           D PPGT D  +++ Q    T    A+IVTTPQ+++ IDV K V     + V  + VVENM
Sbjct: 292 DMPPGTGDIQLTLCQVAPLT---AAVIVTTPQKLAFIDVAKGVRMFSKLKVPCVAVVENM 346

[Close]

BLAST of CSPI04G20620 vs. TAIR 10
Match: AT5G24020.1 (septum site-determining protein (MIND) )

HSP 1 Score: 55.8 bits (133), Expect = 6.8e-08
Identity = 46/182 (25.27%), Postives = 83/182 (45.60%), Query Frame = 0


Query: 8   ILVLSGKGGVGKSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQ------DVH 67
           +++ SGKGGVGK+T +A +  +LA   F V  +D D+   ++  +LGLE +      +V 
Sbjct: 61  VVITSGKGGVGKTTTTANVGLSLARYGFSVVAIDADLGLRNLDLLLGLENRVNYTCVEVI 120

Query: 68  QSNLGWSPVYIE----SNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDF 127
             +       +     SN  ++ I    P     + + G     L+   LK    G  DF
Sbjct: 121 NGDCRLDQALVRDKRWSNFELLCIS--KPRSKLPMGFGGKALEWLV-DALKTRPEGSPDF 180

Query: 128 LVVDAPPGTSDEHISIVQCLKATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVKVLGVV 180
           +++D P G     I+ +     T  + A++VTTP   +L D  +     +  G++ + ++
Sbjct: 181 IIIDCPAGIDAGFITAI-----TPANEAVLVTTPDITALRDADRVTGLLECDGIRDIKMI 234

The following BLAST results are available for this feature:

BLAST of CSPI04G20620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8H1Q2	6.6e-133	80.28	Cytosolic Fe-S cluster assembly factor NBP35 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
A7RUD5	2.1e-86	55.52	Cytosolic Fe-S cluster assembly factor NUBP1 homolog OS=Nematostella vectensis O...	[more]
Q6P298	6.1e-86	54.51	Cytosolic Fe-S cluster assembly factor nubp1 OS=Danio rerio OX=7955 GN=nubp1 PE=...	[more]
Q5I0L4	4.3e-84	53.82	Cytosolic Fe-S cluster assembly factor NUBP1 OS=Rattus norvegicus OX=10116 GN=Nu...	[more]
Q3KQF0	5.7e-84	53.47	Cytosolic Fe-S cluster assembly factor nubp1-A OS=Xenopus laevis OX=8355 GN=nubp...	[more]

BLAST of CSPI04G20620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L2L1	1.3e-171	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G540120 PE=3 SV=1	[more]
A0A1S3B5W7	3.7e-171	99.67	Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A5D3D0N0	3.7e-171	99.67	Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A6J1JQ09	3.6e-158	92.38	Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1ERE1	3.6e-158	92.05	Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of CSPI04G20620 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8649747.1	2.6e-171	100.00	hypothetical protein Csa_012684 [Cucumis sativus]	[more]
XP_004144673.1	2.6e-171	100.00	cytosolic Fe-S cluster assembly factor NBP35 [Cucumis sativus] >XP_011653919.1 c...	[more]
XP_008442190.1	7.7e-171	99.67	PREDICTED: cytosolic Fe-S cluster assembly factor NBP35 [Cucumis melo] >XP_00844...	[more]
KAA0041216.1	7.7e-171	99.67	cytosolic Fe-S cluster assembly factor NBP35 [Cucumis melo var. makuwa] >TYK1601...	[more]
XP_038883029.1	7.5e-166	97.02	cytosolic Fe-S cluster assembly factor NBP35 [Benincasa hispida]	[more]

BLAST of CSPI04G20620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50960.1	4.7e-134	80.28	nucleotide binding protein 35	[more]
AT4G19540.1	9.4e-34	42.86	IND1(iron-sulfur protein required for NADH dehydrogenase)-like	[more]
AT3G24430.1	2.4e-29	39.44	ATP binding	[more]
AT5G24020.1	6.8e-08	25.27	septum site-determining protein (MIND)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR033756	Flagellum site-determining protein YlxH/ Fe-S cluster assembling factor NBP35	PFAM	PF10609	ParA	coord: 4..187 e-value: 9.8E-82 score: 274.1 coord: 226..287 e-value: 8.0E-7 score: 28.8
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 1..298 e-value: 6.0E-89 score: 300.2
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 3..267
None	No IPR available	PANTHER	PTHR23264:SF36	CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NBP35	coord: 1..289
None	No IPR available	CDD	cd02037	Mrp_NBP35	coord: 6..259 e-value: 1.19065E-118 score: 337.937
IPR019591	Mrp/NBP35 ATP-binding protein	PANTHER	PTHR23264	NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED	coord: 1..289
IPR019591	Mrp/NBP35 ATP-binding protein	HAMAP	MF_02040	Mrp_NBP35	coord: 1..288 score: 34.252247
IPR000808	Mrp, conserved site	PROSITE	PS01215	MRP	coord: 112..128

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G20620.1	CSPI04G20620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
cellular_component	GO:0005829	cytosol
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0051536	iron-sulfur cluster binding