CSPI04G15590 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI04G15590
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionmitochondrial uncoupling protein 1
LocationChr4: 12934277 .. 12938253 (+)
RNA-Seq ExpressionCSPI04G15590
SyntenyCSPI04G15590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCAGAGTGGCGTAGTAGGCCGAAACCCAAGAAAAAGGAGCTGCCAAGACTAACAAACAAAGCTTCCCTAAGCCATTTCTTTCTCGTTTTCCTTTGCTTTTCCCCAGATTCCCACCTCAACGACGAGACCCATTATTCTTCTTCTTCCATTCCTTTGTTCTATTTCCATTTTTTTTGTTTTTTCTCTCCGAATTCCTCTTGATCTCCTTCTCTCCGGTTCAGTCTCGTGGGTTTCTTTTCTTTTCCCCCTTGGCTGCTCCCGATTTAGCCATGGTTGCCGATTCGAAAGGCAAATCGGACATCTCATTTGCCGGCACCTTTGCCAGCAGTGCTTTTGCTGCTTCTTTCGCTGAGGTAATGTATTACATACATATTCTGCTTTGCATCTTCTTAAGCCCTTTTGTTGTTTGTGTTCTGTAGAGTTTCTTGTTGAAATTTTGTTTTTGTTTATTTTTTTGTTTGGGGTTAATGAGTTTAGTGTCTGGTGTGTTTTGTAGTTGTCAAAGTCAAATGATAAACATGGGTTTGTGAGATAATTGGCTGATTTTATCTCCATCTTCACCATTTTGAACTTTGGTTGATAATGTATTAGGATTGGCTTAAGCGATTTTGGTTTTAAAAAAGGTTTTTTTTTTTTTTTTGTTGGATGGGGTTGGAGTTTGACTGCTGTTTGGAGGGATCGTGACCATCATTAACAGTTAGAACTGATTTCTTATTACTTTTATTGATTGAAAGTGTGATTTTGTTACTATTTGCTTTCTTGTGTTTGTTTGGGAAACCATGGAAATCGATCTATGTTGTTGTTAACGTTTGCTAGGGGGTGTTTCTTGTTTTTAGAAATTTGCTAAAATTGTACAAGTGTTCTTAAAAGGTGGAATGCTAAACAAATAACATGGCTACGTAACGAGTAGATATTTTGAACTTAATGATCGAAAACAGGTAATTGAAACTAAAAATAAAATTGTTATGCTTTATGCACTCACGAAACTGTTGTGATCTTCGTCCACCTTACACTGTTGGATCTGAAATGATTTTTGACAATAACTGGCATGCATTTGATATTGATGCAGATATGCACCATTCCATTAGACACTGCCAAGGTTAGGCTTCAGCTGCAAAAGAAAGCGGTTGCTGGTGATGTCTTACCAAAATATAGGGGCATGCTGGGCACAGTTGCAACCATAGCCAGAGAAGAAGGTTTGGCGTCCTTGTGGAAAGGCATTGTTCCTGGATTGCATCGCCAATGCTTATTTGGAGGCTTAAGGATTGGGATGTATGAGCCTGTAAGTTTTCGAGTCCTGTCTGGCCGTTTCTACTGTTTTGTCTTTTAGCTCATATCTAACACAAACTTTTATACTGTTTACATCACAGGTCAAAAACTTTTATGTGGGCAGTGACTTTGTGGGTGATGTTCCTTTGTCGAAGAAGATTCTTGCTGCACTGACAACTGGTGAGGATGGATTAAACTTTTTTCCCATGTGGAACAGTTTTTCTTGAATCCACCATCGCATGTTGATGGTTGCAATATTTGTTTTTAGCATTCATGCCTGTGTTTCTTGTTGAGAACGATAGAAAAACTTCAAAGTATCAGGATTTAGAGTTTCTCAAATGTCAAATAATAATAATAACAGTAAATAAACCATCCATAAAGGAAAAGAATTGGCCTATAAATTCTTGATCAAGACATGCTCTTCGGCATTCCTCTTCCAAGTTATAATAGTCTGTTAAAACTCTTATGCAGTCATGTCCCCATAAAACGCTCATTGCCTTTAAGCTAAAAGAAAACTATGAATAAGAGGATGTTTTGACAAGATTGAACCAGAGAAAATTTGAAAAAAAAGGAAGAAATGATTTCATTGGCTTTGGAAGTAATTGGGGTGCTACATAAACATTTCTTCCAAGTGGTTATTGTATTCAACTTCTCGCTCATGTTTTAGAAACTCAACCAATTTGATGTAGTAAAATGAGACGATTTAAAAATCTGGTCAGAATGATCTCTAAGGGCTTGAAAACATTTTTTTAAAACTTATGGTTTCTTTTAGTACTTAAAAAGATGTTCCATACAGGTCTGGTCAACTAAAATCATCCGAAAAAGGAAGGAGTGGAAGTTAATTTACTAATTTTCCTATCCTCCTGTTAACAGGTGCTTTAGGGATCACAATAGCTAATCCAACTGATCTTGTGAAAGTTCGACTTCAAGCGGAAGGAAAATTACCTCCTGGTGCGCCTAGGCGTTATTCTGGGGCATTGAATGCTTATTCAACCATTGTGAGACAGGTTAGCTATTTGACCTTAAGATCAGAAAATTTTTTGTTGCCATTTCCATTAGATTGATTTTCATTTAACATATAGGAAGGAGTTGGTGCTCTATGGACTGGCATTGGGCCAAATATAGCAAGAAATGCGATTATAAATGCTGCAGAACTAGCAAGCTACGATCAAGTGAAACAGGTAAAGGCATTTGAAATTTGTGTTCTTAGAACTAATCTTGTTTCCTGCCCTTAACGCATCACGTGAAATAGGTAAAAGCATTAGACTTTTTGTGCCAATTCTTATCTTATTCTGTTTGTTGCAGACAATTTTGAAAATTCCAGGTTTCACGGATAATGTTGTTACGCATCTTCTTGCTGGTCTTGGAGCAGGGTTTTTTGCTGTATGCATTGGCTCCCCGGTCGATGTGGTAATCTTTCTATATCTATGATATATCGCCTCTTTCATTAGGGTTTTCTTCTAAGAATTTCTAGGTGTCACACTCACACCCTCTTTCAAATATAATTCATGCCGTTTCATGGGACTCTTAAGTCATTTGCACATGATCGATCATATACTTCTTTGGCAGGTGAAGTCAAGAATGATGGGAGATTCAACTTACAAAAGCACACTTGATTGTTTCGTCAAGACTTTAAGAAATGATGTATGTTTTCATACTCTTCCTTTGTTCCCATTAACTTAGTTGAGTCTGTTTTCTTGATTATCAGTTTTTTAAATTAAAAAAATGTAAATATATTATATGGGATTTGCATTTTGCAGGGACCTCTAGCATTTTATAAGGGCTTTATCCCAAATTTTGGTCGGCTAGGATCATGGAATGTGATCATGTTCCTAACTCTAGAACAGGTACTTACTTTCGACCCCTATGCTTTTGATTTCAAATTGTGATCAGCTACACAATTACTAAGAATTACTTAAAGAAAATAAAACATGTTAGAAAATAAAACATGTTTCTCAAAAACCTTATTTTCTTATTTGTGTTAGAACCAAAATGATTGAAAATTAAACTAGGAAAGAAGCAATACGTCAAAAAGAAAGAATTTGCTGATTCTTTTTCTCCTGTCTGAACATAACCAAGAATTATCAAAGCCATTGTTATCAAATTTTCTTCTAAGAGGCAATACATACATGTAATCTAATGAGTTATTGAGTGATTCAATTGTTTGTCATGTGATAGGCCAAGAAGTTTGTGAGAAATATAGAATCTTCATCTTGAGCTATGGGGGAAGTGTTGTGTAAGCTGGATTGCTCCCTTTTCAAACTATTGCTTAGAGAATAAAAATCACCCCACAGGAATAATTTTTACTTTATGTGCTGATTCCATTGATGACGTGAAATATTCTGCTCCGGCCATTTGAGAGAATAAGATGAAAGTAATCCTCAGACCGATGGTTCTTTCTCAATGTTGCCATTCATTTTCTGTACTACAAAAGAATTCGTCATTCTGCTAATCTTATTAAACTATTTTCTTAAATAGTATTCCAATTTTCAAGTGCAATGGTTTAGACTACAATCTTATTGTATTCTAGTCCATTTGAAACATCTTTAAACAAATATTTTTTTAAGTTTCATTGATATTTGGCCTTTTTTCTTACCTTCAGTTGGGAAGCTCGAGTTTATGTATAATCAAACTATAGTTGTTTGGCCTAGTGTCTATACAAGTTACGTGGGTGTAATAAATAACTTTAATTTATGGTGATTACGTA

mRNA sequence

GTCAGAGTGGCGTAGTAGGCCGAAACCCAAGAAAAAGGAGCTGCCAAGACTAACAAACAAAGCTTCCCTAAGCCATTTCTTTCTCGTTTTCCTTTGCTTTTCCCCAGATTCCCACCTCAACGACGAGACCCATTATTCTTCTTCTTCCATTCCTTTGTTCTATTTCCATTTTTTTTGTTTTTTCTCTCCGAATTCCTCTTGATCTCCTTCTCTCCGGTTCAGTCTCGTGGGTTTCTTTTCTTTTCCCCCTTGGCTGCTCCCGATTTAGCCATGGTTGCCGATTCGAAAGGCAAATCGGACATCTCATTTGCCGGCACCTTTGCCAGCAGTGCTTTTGCTGCTTCTTTCGCTGAGATATGCACCATTCCATTAGACACTGCCAAGGTTAGGCTTCAGCTGCAAAAGAAAGCGGTTGCTGGTGATGTCTTACCAAAATATAGGGGCATGCTGGGCACAGTTGCAACCATAGCCAGAGAAGAAGGTTTGGCGTCCTTGTGGAAAGGCATTGTTCCTGGATTGCATCGCCAATGCTTATTTGGAGGCTTAAGGATTGGGATGTATGAGCCTGTCAAAAACTTTTATGTGGGCAGTGACTTTGTGGGTGATGTTCCTTTGTCGAAGAAGATTCTTGCTGCACTGACAACTGGTGCTTTAGGGATCACAATAGCTAATCCAACTGATCTTGTGAAAGTTCGACTTCAAGCGGAAGGAAAATTACCTCCTGGTGCGCCTAGGCGTTATTCTGGGGCATTGAATGCTTATTCAACCATTGTGAGACAGGAAGGAGTTGGTGCTCTATGGACTGGCATTGGGCCAAATATAGCAAGAAATGCGATTATAAATGCTGCAGAACTAGCAAGCTACGATCAAGTGAAACAGACAATTTTGAAAATTCCAGGTTTCACGGATAATGTTGTTACGCATCTTCTTGCTGGTCTTGGAGCAGGGTTTTTTGCTGTATGCATTGGCTCCCCGGTCGATGTGGTGAAGTCAAGAATGATGGGAGATTCAACTTACAAAAGCACACTTGATTGTTTCGTCAAGACTTTAAGAAATGATGGACCTCTAGCATTTTATAAGGGCTTTATCCCAAATTTTGGTCGGCTAGGATCATGGAATGTGATCATGTTCCTAACTCTAGAACAGGCCAAGAAGTTTGTGAGAAATATAGAATCTTCATCTTGAGCTATGGGGGAAGTGTTGTGTAAGCTGGATTGCTCCCTTTTCAAACTATTGCTTAGAGAATAAAAATCACCCCACAGGAATAATTTTTACTTTATGTGCTGATTCCATTGATGACGTGAAATATTCTGCTCCGGCCATTTGAGAGAATAAGATGAAAGTAATCCTCAGACCGATGGTTCTTTCTCAATGTTGCCATTCATTTTCTGTACTACAAAAGAATTCGTCATTCTGCTAATCTTATTAAACTATTTTCTTAAATAGTATTCCAATTTTCAAGTGCAATGGTTTAGACTACAATCTTATTGTATTCTAGTCCATTTGAAACATCTTTAAACAAATATTTTTTTAAGTTTCATTGATATTTGGCCTTTTTTCTTACCTTCAGTTGGGAAGCTCGAGTTTATGTATAATCAAACTATAGTTGTTTGGCCTAGTGTCTATACAAGTTACGTGGGTGTAATAAATAACTTTAATTTATGGTGATTACGTA

Coding sequence (CDS)

ATGGTTGCCGATTCGAAAGGCAAATCGGACATCTCATTTGCCGGCACCTTTGCCAGCAGTGCTTTTGCTGCTTCTTTCGCTGAGATATGCACCATTCCATTAGACACTGCCAAGGTTAGGCTTCAGCTGCAAAAGAAAGCGGTTGCTGGTGATGTCTTACCAAAATATAGGGGCATGCTGGGCACAGTTGCAACCATAGCCAGAGAAGAAGGTTTGGCGTCCTTGTGGAAAGGCATTGTTCCTGGATTGCATCGCCAATGCTTATTTGGAGGCTTAAGGATTGGGATGTATGAGCCTGTCAAAAACTTTTATGTGGGCAGTGACTTTGTGGGTGATGTTCCTTTGTCGAAGAAGATTCTTGCTGCACTGACAACTGGTGCTTTAGGGATCACAATAGCTAATCCAACTGATCTTGTGAAAGTTCGACTTCAAGCGGAAGGAAAATTACCTCCTGGTGCGCCTAGGCGTTATTCTGGGGCATTGAATGCTTATTCAACCATTGTGAGACAGGAAGGAGTTGGTGCTCTATGGACTGGCATTGGGCCAAATATAGCAAGAAATGCGATTATAAATGCTGCAGAACTAGCAAGCTACGATCAAGTGAAACAGACAATTTTGAAAATTCCAGGTTTCACGGATAATGTTGTTACGCATCTTCTTGCTGGTCTTGGAGCAGGGTTTTTTGCTGTATGCATTGGCTCCCCGGTCGATGTGGTGAAGTCAAGAATGATGGGAGATTCAACTTACAAAAGCACACTTGATTGTTTCGTCAAGACTTTAAGAAATGATGGACCTCTAGCATTTTATAAGGGCTTTATCCCAAATTTTGGTCGGCTAGGATCATGGAATGTGATCATGTTCCTAACTCTAGAACAGGCCAAGAAGTTTGTGAGAAATATAGAATCTTCATCTTGA

Protein sequence

MVADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGMLGTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKILAALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVKSRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNIESSS*
Homology
BLAST of CSPI04G15590 vs. ExPASy Swiss-Prot
Match: O81845 (Mitochondrial uncoupling protein 1 OS=Arabidopsis thaliana OX=3702 GN=PUMP1 PE=1 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 4.7e-147
Identity = 258/299 (86.29%), Postives = 277/299 (92.64%), Query Frame = 0

Query: 7   GKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDV-LPKYRGMLGTVAT 66
           GKSD+S   TFA SAFAA   E+CTIPLDTAKVRLQLQK A+AGDV LPKYRG+LGTV T
Sbjct: 5   GKSDLSLPKTFACSAFAACVGEVCTIPLDTAKVRLQLQKSALAGDVTLPKYRGLLGTVGT 64

Query: 67  IAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKILAALTT 126
           IAREEGL SLWKG+VPGLHRQCLFGGLRIGMYEPVKN YVG DFVGDVPLSKKILA LTT
Sbjct: 65  IAREEGLRSLWKGVVPGLHRQCLFGGLRIGMYEPVKNLYVGKDFVGDVPLSKKILAGLTT 124

Query: 127 GALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGIGPNIA 186
           GALGI +ANPTDLVKVRLQAEGKL  GAPRRYSGALNAYSTIVRQEGV ALWTG+GPN+A
Sbjct: 125 GALGIMVANPTDLVKVRLQAEGKLAAGAPRRYSGALNAYSTIVRQEGVRALWTGLGPNVA 184

Query: 187 RNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVKSRMMG 246
           RNAIINAAELASYDQVK+TILKIPGFTDNVVTH+L+GLGAGFFAVCIGSPVDVVKSRMMG
Sbjct: 185 RNAIINAAELASYDQVKETILKIPGFTDNVVTHILSGLGAGFFAVCIGSPVDVVKSRMMG 244

Query: 247 DS-TYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNIESS 304
           DS  YK T+DCFVKTL++DGP+AFYKGFIPNFGRLGSWNVIMFLTLEQAKK+VR +++S
Sbjct: 245 DSGAYKGTIDCFVKTLKSDGPMAFYKGFIPNFGRLGSWNVIMFLTLEQAKKYVRELDAS 303

BLAST of CSPI04G15590 vs. ExPASy Swiss-Prot
Match: Q9ZWG1 (Mitochondrial uncoupling protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUMP2 PE=2 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 6.4e-128
Identity = 219/302 (72.52%), Postives = 263/302 (87.09%), Query Frame = 0

Query: 2   VADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGD--VLPKYRGM 61
           +AD K + +ISF  TF  SAFAA FAE+CTIPLDTAKVRLQLQ+K   GD   LPKYRG 
Sbjct: 1   MADFKPRIEISFLETFICSAFAACFAELCTIPLDTAKVRLQLQRKIPTGDGENLPKYRGS 60

Query: 62  LGTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKI 121
           +GT+ATIAREEG++ LWKG++ GLHRQC++GGLRIG+YEPVK   VGSDF+GD+PL +KI
Sbjct: 61  IGTLATIAREEGISGLWKGVIAGLHRQCIYGGLRIGLYEPVKTLLVGSDFIGDIPLYQKI 120

Query: 122 LAALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTG 181
           LAAL TGA+ I +ANPTDLVKVRLQ+EGKLP G PRRY+GA++AY TIV+ EGV ALWTG
Sbjct: 121 LAALLTGAIAIIVANPTDLVKVRLQSEGKLPAGVPRRYAGAVDAYFTIVKLEGVSALWTG 180

Query: 182 IGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVV 241
           +GPNIARNAI+NAAELASYDQ+K+TI+KIP F D+V+THLLAGL AGFFAVCIGSP+DVV
Sbjct: 181 LGPNIARNAIVNAAELASYDQIKETIMKIPFFRDSVLTHLLAGLAAGFFAVCIGSPIDVV 240

Query: 242 KSRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKK-FVR 301
           KSRMMGDSTY++T+DCF+KT++ +G +AFYKGF+PNF RLG+WN IMFLTLEQ KK F+R
Sbjct: 241 KSRMMGDSTYRNTVDCFIKTMKTEGIMAFYKGFLPNFTRLGTWNAIMFLTLEQVKKVFLR 300

BLAST of CSPI04G15590 vs. ExPASy Swiss-Prot
Match: P56501 (Mitochondrial uncoupling protein 3 OS=Mus musculus OX=10090 GN=Ucp3 PE=1 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 1.7e-72
Identity = 141/289 (48.79%), Postives = 191/289 (66.09%), Query Frame = 0

Query: 17  FASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGMLGTVATIAREEGLASLW 76
           F  +  AA FA++ T PLDTAKVRLQ+Q +   G    +YRG+LGT+ T+ R EG  S +
Sbjct: 17  FLGAGTAACFADLLTFPLDTAKVRLQIQGEN-PGAQSVQYRGVLGTILTMVRTEGPRSPY 76

Query: 77  KGIVPGLHRQCLFGGLRIGMYEPVKNFYV--GSDFVGDVPLSKKILAALTTGALGITIAN 136
            G+V GLHRQ  F  +RIG+Y+ VK FY   G+D      ++ +ILA  TTGA+ +T A 
Sbjct: 77  SGLVAGLHRQMSFASIRIGLYDSVKQFYTPKGADHSS---VAIRILAGCTTGAMAVTCAQ 136

Query: 137 PTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGIGPNIARNAIINAAE 196
           PTD+VKVR QA  +L  G  R+Y G ++AY TI R+EGV  LW G  PNI RNAI+N AE
Sbjct: 137 PTDVVKVRFQAMIRLGTGGERKYRGTMDAYRTIAREEGVRGLWKGTWPNITRNAIVNCAE 196

Query: 197 LASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVKSRMMGD--STYKST 256
           + +YD +K+ +L+   FTDN   H ++  GAGF A  + SPVDVVK+R M      Y+S 
Sbjct: 197 MVTYDIIKEKLLESHLFTDNFPCHFVSAFGAGFCATVVASPVDVVKTRYMNAPLGRYRSP 256

Query: 257 LDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNIE 302
           L C +K +  +GP AFYKGF+P+F RLG+WNV+MF+T EQ K+ +  ++
Sbjct: 257 LHCMLKMVAQEGPTAFYKGFVPSFLRLGAWNVMMFVTYEQLKRALMKVQ 301

BLAST of CSPI04G15590 vs. ExPASy Swiss-Prot
Match: P56499 (Mitochondrial uncoupling protein 3 OS=Rattus norvegicus OX=10116 GN=Ucp3 PE=2 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 5.0e-72
Identity = 141/289 (48.79%), Postives = 190/289 (65.74%), Query Frame = 0

Query: 17  FASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGMLGTVATIAREEGLASLW 76
           F  +  AA FA++ T PLDTAKVRLQ+Q +   G    +YRG+LGT+ T+ R EG  S +
Sbjct: 17  FLGAGTAACFADLLTFPLDTAKVRLQIQGEN-PGVQSVQYRGVLGTILTMVRTEGPRSPY 76

Query: 77  KGIVPGLHRQCLFGGLRIGMYEPVKNFYV--GSDFVGDVPLSKKILAALTTGALGITIAN 136
            G+V GLHRQ  F  +RIG+Y+ VK FY   G+D      ++ +ILA  TTGA+ +T A 
Sbjct: 77  SGLVAGLHRQMSFASIRIGLYDSVKQFYTPKGTDHSS---VAIRILAGCTTGAMAVTCAQ 136

Query: 137 PTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGIGPNIARNAIINAAE 196
           PTD+VKVR QA  +L  G  R+Y G ++AY TI R+EGV  LW G  PNI RNAI+N AE
Sbjct: 137 PTDVVKVRFQAMIRLGTGGERKYRGTMDAYRTIAREEGVRGLWKGTWPNITRNAIVNCAE 196

Query: 197 LASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVKSRMMG--DSTYKST 256
           + +YD +K+ +L    FTDN   H ++  GAGF A  + SPVDVVK+R M      Y+S 
Sbjct: 197 MVTYDIIKEKLLDSHLFTDNFPCHFVSAFGAGFCATVVASPVDVVKTRYMNAPPGRYRSP 256

Query: 257 LDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNIE 302
           L C ++ +  +GP AFYKGF+P+F RLGSWNV+MF+T EQ K+ +  ++
Sbjct: 257 LHCMLRMVAQEGPTAFYKGFMPSFLRLGSWNVMMFVTYEQLKRALMKVQ 301

BLAST of CSPI04G15590 vs. ExPASy Swiss-Prot
Match: Q9N2I9 (Mitochondrial uncoupling protein 3 OS=Canis lupus familiaris OX=9615 GN=UCP3 PE=2 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 6.6e-72
Identity = 142/291 (48.80%), Postives = 189/291 (64.95%), Query Frame = 0

Query: 17  FASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPK--YRGMLGTVATIAREEGLAS 76
           F  +  AA FA++ T PLDTAKVRLQ+Q +  A     +  YRG+LGT+ T+ R EG  S
Sbjct: 17  FLGAGTAACFADLLTFPLDTAKVRLQIQGENQATQAARRIQYRGVLGTILTMVRTEGPRS 76

Query: 77  LWKGIVPGLHRQCLFGGLRIGMYEPVKNFYV--GSDFVGDVPLSKKILAALTTGALGITI 136
            + G+V GL RQ  F  +RIG+Y+ VK FY   GSD      ++ +ILA  TTGA+ ++ 
Sbjct: 77  PYNGLVAGLQRQMSFASIRIGLYDSVKQFYTPKGSDHSS---ITTRILAGCTTGAMAVSC 136

Query: 137 ANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGIGPNIARNAIINA 196
           A PTD+VKVR QA   L  G+ R+YSG ++AY TI R+EGV  LW G  PNI RNAI+N 
Sbjct: 137 AQPTDVVKVRFQASIHLGAGSNRKYSGTMDAYRTIAREEGVRGLWKGTLPNITRNAIVNC 196

Query: 197 AELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVKSRMMGD--STYK 256
           AE+ +YD +K+ +L     TDN   HL++  GAGF A  + SPVDVVK+R M      Y 
Sbjct: 197 AEMVTYDIIKEKLLDYHLLTDNFPCHLISAFGAGFCATVVASPVDVVKTRYMNSPPGQYC 256

Query: 257 STLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNIE 302
           S LDC +K +  +GP AFYKGF P+F RLG+WNV+MF+T EQ K+ +  ++
Sbjct: 257 SPLDCMLKMVTQEGPTAFYKGFTPSFLRLGTWNVVMFVTYEQLKRALMKVQ 304

BLAST of CSPI04G15590 vs. ExPASy TrEMBL
Match: A0A0A0KXV2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G307900 PE=3 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 2.7e-169
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML 60
           MVADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML 60

Query: 61  GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 120
           GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL
Sbjct: 61  GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 120

Query: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI 180
           AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI
Sbjct: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI 180

Query: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 240
           GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK
Sbjct: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 240

Query: 241 SRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI 300
           SRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI
Sbjct: 241 SRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI 300

Query: 301 ESSS 305
           ESSS
Sbjct: 301 ESSS 304

BLAST of CSPI04G15590 vs. ExPASy TrEMBL
Match: A0A5A7TT48 (Mitochondrial uncoupling protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold78209G00510 PE=3 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.3e-168
Identity = 302/304 (99.34%), Postives = 303/304 (99.67%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML 60
           MVADSKGKSDISFAGTFASSAFAA FAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML 60

Query: 61  GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 120
           GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL
Sbjct: 61  GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 120

Query: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI 180
           AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI
Sbjct: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI 180

Query: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 240
           GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK
Sbjct: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 240

Query: 241 SRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI 300
           SRMMGDSTYKSTLDCF+KTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI
Sbjct: 241 SRMMGDSTYKSTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI 300

Query: 301 ESSS 305
           ESSS
Sbjct: 301 ESSS 304

BLAST of CSPI04G15590 vs. ExPASy TrEMBL
Match: A0A1S3BLL8 (mitochondrial uncoupling protein 1 OS=Cucumis melo OX=3656 GN=LOC103490980 PE=3 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.3e-168
Identity = 302/304 (99.34%), Postives = 303/304 (99.67%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML 60
           MVADSKGKSDISFAGTFASSAFAA FAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML 60

Query: 61  GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 120
           GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL
Sbjct: 61  GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 120

Query: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI 180
           AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI
Sbjct: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI 180

Query: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 240
           GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK
Sbjct: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 240

Query: 241 SRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI 300
           SRMMGDSTYKSTLDCF+KTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI
Sbjct: 241 SRMMGDSTYKSTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI 300

Query: 301 ESSS 305
           ESSS
Sbjct: 301 ESSS 304

BLAST of CSPI04G15590 vs. ExPASy TrEMBL
Match: A0A6J1IM65 (mitochondrial uncoupling protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478283 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.4e-164
Identity = 291/304 (95.72%), Postives = 299/304 (98.36%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML 60
           MVADSKGKSDISFAGTFASSAFAA FAE+CTIPLDTAKVRLQLQKKAVAGD LPKYRGML
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAEVCTIPLDTAKVRLQLQKKAVAGDALPKYRGML 60

Query: 61  GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 120
           GTVATIAREEGLASLWKGIVPGLHRQC++GGLRIG+YEPVK FYVGSDFVGDVPLSKKIL
Sbjct: 61  GTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFVGDVPLSKKIL 120

Query: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI 180
           AALTTGALGITIANPTDLVKVRLQAEGKLPPG PRRYSGAL+AYSTIVRQEGVGALWTGI
Sbjct: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQEGVGALWTGI 180

Query: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 240
           GPNIARNAIINAAELASYDQVKQTILKIPGFTDNV+THLLAGLGAGFFAVCIGSPVDVVK
Sbjct: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAVCIGSPVDVVK 240

Query: 241 SRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI 300
           SRMMGDSTYK+TLDCF+KTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVR I
Sbjct: 241 SRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRKI 300

Query: 301 ESSS 305
           ESSS
Sbjct: 301 ESSS 304

BLAST of CSPI04G15590 vs. ExPASy TrEMBL
Match: A0A6J1GFM7 (mitochondrial uncoupling protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111453723 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.4e-164
Identity = 291/304 (95.72%), Postives = 299/304 (98.36%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML 60
           MVADSKGKSDISFAGTFASSAFAA FAE+CTIPLDTAKVRLQLQKKAVAGD LPKYRGML
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAEVCTIPLDTAKVRLQLQKKAVAGDALPKYRGML 60

Query: 61  GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 120
           GTVATIAREEGLASLWKGIVPGLHRQC++GGLRIG+YEPVK FYVGSDFVGDVPLSKKIL
Sbjct: 61  GTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFVGDVPLSKKIL 120

Query: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI 180
           AALTTGALGITIANPTDLVKVRLQAEGKLPPG PRRYSGAL+AYSTIVRQEGVGALWTGI
Sbjct: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQEGVGALWTGI 180

Query: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 240
           GPNIARNAIINAAELASYDQVKQTILKIPGFTDNV+THLLAGLGAGFFAVCIGSPVDVVK
Sbjct: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAVCIGSPVDVVK 240

Query: 241 SRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI 300
           SRMMGDSTYK+TLDCF+KTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVR I
Sbjct: 241 SRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRKI 300

Query: 301 ESSS 305
           ESSS
Sbjct: 301 ESSS 304

BLAST of CSPI04G15590 vs. NCBI nr
Match: XP_004147893.1 (mitochondrial uncoupling protein 1 [Cucumis sativus] >KGN54348.1 hypothetical protein Csa_018142 [Cucumis sativus])

HSP 1 Score: 604.4 bits (1557), Expect = 5.5e-169
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML 60
           MVADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML 60

Query: 61  GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 120
           GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL
Sbjct: 61  GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 120

Query: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI 180
           AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI
Sbjct: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI 180

Query: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 240
           GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK
Sbjct: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 240

Query: 241 SRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI 300
           SRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI
Sbjct: 241 SRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI 300

Query: 301 ESSS 305
           ESSS
Sbjct: 301 ESSS 304

BLAST of CSPI04G15590 vs. NCBI nr
Match: XP_008448974.1 (PREDICTED: mitochondrial uncoupling protein 1 [Cucumis melo] >KAA0045067.1 mitochondrial uncoupling protein 1 [Cucumis melo var. makuwa] >TYJ96260.1 mitochondrial uncoupling protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 602.1 bits (1551), Expect = 2.8e-168
Identity = 302/304 (99.34%), Postives = 303/304 (99.67%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML 60
           MVADSKGKSDISFAGTFASSAFAA FAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML 60

Query: 61  GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 120
           GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL
Sbjct: 61  GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 120

Query: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI 180
           AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI
Sbjct: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI 180

Query: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 240
           GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK
Sbjct: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 240

Query: 241 SRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI 300
           SRMMGDSTYKSTLDCF+KTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI
Sbjct: 241 SRMMGDSTYKSTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI 300

Query: 301 ESSS 305
           ESSS
Sbjct: 301 ESSS 304

BLAST of CSPI04G15590 vs. NCBI nr
Match: XP_038880991.1 (mitochondrial uncoupling protein 1 [Benincasa hispida])

HSP 1 Score: 596.3 bits (1536), Expect = 1.5e-166
Identity = 296/304 (97.37%), Postives = 301/304 (99.01%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML 60
           MVADSKGKSDISFAGTFASSAFAA FAE+CTIPLDTAKVRLQLQKKAVAGDVLPKYRGML
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAEVCTIPLDTAKVRLQLQKKAVAGDVLPKYRGML 60

Query: 61  GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 120
           GTVATIAREEGLASLWKGIVPGLHRQC+FGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL
Sbjct: 61  GTVATIAREEGLASLWKGIVPGLHRQCVFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 120

Query: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI 180
           AALTTGALGIT+ANPTDLVKVRLQAEGKLPPG PRRYSGALNAYSTIVRQEG+GALWTGI
Sbjct: 121 AALTTGALGITVANPTDLVKVRLQAEGKLPPGVPRRYSGALNAYSTIVRQEGIGALWTGI 180

Query: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 240
           GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK
Sbjct: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 240

Query: 241 SRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI 300
           SRMMGDS YKSTLDCF+KTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI
Sbjct: 241 SRMMGDSAYKSTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI 300

Query: 301 ESSS 305
           ESSS
Sbjct: 301 ESSS 304

BLAST of CSPI04G15590 vs. NCBI nr
Match: XP_022950708.1 (mitochondrial uncoupling protein 1-like [Cucurbita moschata] >XP_022978241.1 mitochondrial uncoupling protein 1-like [Cucurbita maxima] >KAG6603605.1 Mitochondrial uncoupling protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 587.0 bits (1512), Expect = 9.2e-164
Identity = 291/304 (95.72%), Postives = 299/304 (98.36%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML 60
           MVADSKGKSDISFAGTFASSAFAA FAE+CTIPLDTAKVRLQLQKKAVAGD LPKYRGML
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAEVCTIPLDTAKVRLQLQKKAVAGDALPKYRGML 60

Query: 61  GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 120
           GTVATIAREEGLASLWKGIVPGLHRQC++GGLRIG+YEPVK FYVGSDFVGDVPLSKKIL
Sbjct: 61  GTVATIAREEGLASLWKGIVPGLHRQCVYGGLRIGLYEPVKTFYVGSDFVGDVPLSKKIL 120

Query: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI 180
           AALTTGALGITIANPTDLVKVRLQAEGKLPPG PRRYSGAL+AYSTIVRQEGVGALWTGI
Sbjct: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGVPRRYSGALSAYSTIVRQEGVGALWTGI 180

Query: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 240
           GPNIARNAIINAAELASYDQVKQTILKIPGFTDNV+THLLAGLGAGFFAVCIGSPVDVVK
Sbjct: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVITHLLAGLGAGFFAVCIGSPVDVVK 240

Query: 241 SRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI 300
           SRMMGDSTYK+TLDCF+KTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVR I
Sbjct: 241 SRMMGDSTYKNTLDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRKI 300

Query: 301 ESSS 305
           ESSS
Sbjct: 301 ESSS 304

BLAST of CSPI04G15590 vs. NCBI nr
Match: XP_022966614.1 (mitochondrial uncoupling protein 1-like [Cucurbita maxima])

HSP 1 Score: 586.3 bits (1510), Expect = 1.6e-163
Identity = 288/304 (94.74%), Postives = 300/304 (98.68%), Query Frame = 0

Query: 1   MVADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYRGML 60
           MVADSKGKSDISFAGTFASSAFAA FAEICTIPLDTAKVRLQLQKKAV GDVLPKYRGML
Sbjct: 1   MVADSKGKSDISFAGTFASSAFAACFAEICTIPLDTAKVRLQLQKKAVVGDVLPKYRGML 60

Query: 61  GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 120
           GTVATIAREEG+ASLWKGIVPGLHRQC+FGGLRIG+YEPVKNFYVGSDFVGDVPL+KKIL
Sbjct: 61  GTVATIAREEGMASLWKGIVPGLHRQCVFGGLRIGLYEPVKNFYVGSDFVGDVPLTKKIL 120

Query: 121 AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI 180
           AALTTGA+GIT+ANPTDLVKVRLQAEGKLPPG PRRYSGALNAYSTIVRQEGVGALWTGI
Sbjct: 121 AALTTGAIGITVANPTDLVKVRLQAEGKLPPGVPRRYSGALNAYSTIVRQEGVGALWTGI 180

Query: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 240
           GPNIARNAIINAAELASYDQVKQTILKIPGFTDNV+THLL+GLGAGFFAVCIGSPVDVVK
Sbjct: 181 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVLTHLLSGLGAGFFAVCIGSPVDVVK 240

Query: 241 SRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNI 300
           SRMMGDSTYKST+DCF+KTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFV+ +
Sbjct: 241 SRMMGDSTYKSTIDCFIKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVKKL 300

Query: 301 ESSS 305
           ESSS
Sbjct: 301 ESSS 304

BLAST of CSPI04G15590 vs. TAIR 10
Match: AT3G54110.1 (plant uncoupling mitochondrial protein 1 )

HSP 1 Score: 521.9 bits (1343), Expect = 3.4e-148
Identity = 258/299 (86.29%), Postives = 277/299 (92.64%), Query Frame = 0

Query: 7   GKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDV-LPKYRGMLGTVAT 66
           GKSD+S   TFA SAFAA   E+CTIPLDTAKVRLQLQK A+AGDV LPKYRG+LGTV T
Sbjct: 5   GKSDLSLPKTFACSAFAACVGEVCTIPLDTAKVRLQLQKSALAGDVTLPKYRGLLGTVGT 64

Query: 67  IAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKILAALTT 126
           IAREEGL SLWKG+VPGLHRQCLFGGLRIGMYEPVKN YVG DFVGDVPLSKKILA LTT
Sbjct: 65  IAREEGLRSLWKGVVPGLHRQCLFGGLRIGMYEPVKNLYVGKDFVGDVPLSKKILAGLTT 124

Query: 127 GALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGIGPNIA 186
           GALGI +ANPTDLVKVRLQAEGKL  GAPRRYSGALNAYSTIVRQEGV ALWTG+GPN+A
Sbjct: 125 GALGIMVANPTDLVKVRLQAEGKLAAGAPRRYSGALNAYSTIVRQEGVRALWTGLGPNVA 184

Query: 187 RNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVKSRMMG 246
           RNAIINAAELASYDQVK+TILKIPGFTDNVVTH+L+GLGAGFFAVCIGSPVDVVKSRMMG
Sbjct: 185 RNAIINAAELASYDQVKETILKIPGFTDNVVTHILSGLGAGFFAVCIGSPVDVVKSRMMG 244

Query: 247 DS-TYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKKFVRNIESS 304
           DS  YK T+DCFVKTL++DGP+AFYKGFIPNFGRLGSWNVIMFLTLEQAKK+VR +++S
Sbjct: 245 DSGAYKGTIDCFVKTLKSDGPMAFYKGFIPNFGRLGSWNVIMFLTLEQAKKYVRELDAS 303

BLAST of CSPI04G15590 vs. TAIR 10
Match: AT5G58970.1 (uncoupling protein 2 )

HSP 1 Score: 458.4 bits (1178), Expect = 4.6e-129
Identity = 219/302 (72.52%), Postives = 263/302 (87.09%), Query Frame = 0

Query: 2   VADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGD--VLPKYRGM 61
           +AD K + +ISF  TF  SAFAA FAE+CTIPLDTAKVRLQLQ+K   GD   LPKYRG 
Sbjct: 1   MADFKPRIEISFLETFICSAFAACFAELCTIPLDTAKVRLQLQRKIPTGDGENLPKYRGS 60

Query: 62  LGTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKI 121
           +GT+ATIAREEG++ LWKG++ GLHRQC++GGLRIG+YEPVK   VGSDF+GD+PL +KI
Sbjct: 61  IGTLATIAREEGISGLWKGVIAGLHRQCIYGGLRIGLYEPVKTLLVGSDFIGDIPLYQKI 120

Query: 122 LAALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTG 181
           LAAL TGA+ I +ANPTDLVKVRLQ+EGKLP G PRRY+GA++AY TIV+ EGV ALWTG
Sbjct: 121 LAALLTGAIAIIVANPTDLVKVRLQSEGKLPAGVPRRYAGAVDAYFTIVKLEGVSALWTG 180

Query: 182 IGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVV 241
           +GPNIARNAI+NAAELASYDQ+K+TI+KIP F D+V+THLLAGL AGFFAVCIGSP+DVV
Sbjct: 181 LGPNIARNAIVNAAELASYDQIKETIMKIPFFRDSVLTHLLAGLAAGFFAVCIGSPIDVV 240

Query: 242 KSRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQAKK-FVR 301
           KSRMMGDSTY++T+DCF+KT++ +G +AFYKGF+PNF RLG+WN IMFLTLEQ KK F+R
Sbjct: 241 KSRMMGDSTYRNTVDCFIKTMKTEGIMAFYKGFLPNFTRLGTWNAIMFLTLEQVKKVFLR 300

BLAST of CSPI04G15590 vs. TAIR 10
Match: AT5G58970.2 (uncoupling protein 2 )

HSP 1 Score: 363.6 bits (932), Expect = 1.5e-100
Identity = 184/273 (67.40%), Postives = 217/273 (79.49%), Query Frame = 0

Query: 2   VADSKGKSDISFAGTFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGD--VLPKYRGM 61
           +AD K + +ISF  TF  SAFAA FAE+CTIPLDTAKVRLQLQ+K   GD   LPKYRG 
Sbjct: 1   MADFKPRIEISFLETFICSAFAACFAELCTIPLDTAKVRLQLQRKIPTGDGENLPKYRGS 60

Query: 62  LGTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKI 121
           +GT+ATIAREEG++ LWKG++ GLHRQC++GGLRIG+YEPVK   VGSDF+GD+PL +KI
Sbjct: 61  IGTLATIAREEGISGLWKGVIAGLHRQCIYGGLRIGLYEPVKTLLVGSDFIGDIPLYQKI 120

Query: 122 LAALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTG 181
           LAAL TGA+ I +ANPTDLVKVRLQ+EGKLP G PRRY+GA++AY TIV+ EGV ALWTG
Sbjct: 121 LAALLTGAIAIIVANPTDLVKVRLQSEGKLPAGVPRRYAGAVDAYFTIVKLEGVSALWTG 180

Query: 182 IGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVV 241
           +GPNIARNAI+NAAELASYDQ+K+TI+KIP F D+V+THLLAGL AGFFAVCIGSP+DVV
Sbjct: 181 LGPNIARNAIVNAAELASYDQIKETIMKIPFFRDSVLTHLLAGLAAGFFAVCIGSPIDVV 240

Query: 242 KSRMMGDSTYKSTLDCFVKTLRNDGPLAFYKGF 273
                    +KST   F   L   G    Y  F
Sbjct: 241 SIHFR--LLHKSTTRLFGFRLIMCGSAYIYNKF 271

BLAST of CSPI04G15590 vs. TAIR 10
Match: AT4G24570.1 (dicarboxylate carrier 2 )

HSP 1 Score: 196.8 bits (499), Expect = 2.5e-50
Identity = 114/309 (36.89%), Postives = 167/309 (54.05%), Query Frame = 0

Query: 16  TFASSAFAASFAEICTIPLDTAKVRLQLQKKAVAGDVLPKYR------------------ 75
           +F     A+  A   T PLD  KVRLQL  +A +   +   R                  
Sbjct: 5   SFVEGGIASVIAGCSTHPLDLIKVRLQLHGEAPSTTTVTLLRPALAFPNSSPAAFLETTS 64

Query: 76  -----GMLGTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGD 135
                G +     I + EG A+L+ G+   L RQ L+   R+G+YE +KN +   +  G 
Sbjct: 65  SVPKVGPISLGINIVKSEGAAALFSGVSATLLRQTLYSTTRMGLYEVLKNKWTDPE-SGK 124

Query: 136 VPLSKKILAALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEG 195
           + LS+KI A L  G +G  + NP D+  VR+QA+G+LP    R Y+G  +A  ++V+ EG
Sbjct: 125 LNLSRKIGAGLVAGGIGAAVGNPADVAMVRMQADGRLPLAQRRNYAGVGDAIRSMVKGEG 184

Query: 196 VGALWTGIGPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCI 255
           V +LW G    I R  I+ AA+LASYDQ K+ IL+     D + TH++A   AGF A   
Sbjct: 185 VTSLWRGSALTINRAMIVTAAQLASYDQFKEGILENGVMNDGLGTHVVASFAAGFVASVA 244

Query: 256 GSPVDVVKSRMMG--DSTYKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTL 300
            +PVDV+K+R+M      Y    DC VKT++ +G +A YKGF+P   R G + V++F+TL
Sbjct: 245 SNPVDVIKTRVMNMKVGAYDGAWDCAVKTVKAEGAMALYKGFVPTVCRQGPFTVVLFVTL 304

BLAST of CSPI04G15590 vs. TAIR 10
Match: AT2G22500.1 (uncoupling protein 5 )

HSP 1 Score: 195.7 bits (496), Expect = 5.5e-50
Identity = 109/308 (35.39%), Postives = 169/308 (54.87%), Query Frame = 0

Query: 17  FASSAFAASFAEICTIPLDTAKVRLQLQ----------------KKAVAGDVLPKYRGML 76
           FA    A+  A   T PLD  KVR+QLQ                + +   +  P   G++
Sbjct: 6   FAEGGIASIVAGCSTHPLDLIKVRMQLQGESAPIQTNLRPALAFQTSTTVNAPPLRVGVI 65

Query: 77  GTVATIAREEGLASLWKGIVPGLHRQCLFGGLRIGMYEPVKNFYVGSDFVGDVPLSKKIL 136
           G  + + REEG+ +L+ G+   + RQ L+   R+G+Y+ +K  +   +    +PL KKI 
Sbjct: 66  GVGSRLIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKGEWTDPE-TKTMPLMKKIG 125

Query: 137 AALTTGALGITIANPTDLVKVRLQAEGKLPPGAPRRYSGALNAYSTIVRQEGVGALWTGI 196
           A    GA+G  + NP D+  VR+QA+G+LP    R Y   L+A + ++R EGV +LW G 
Sbjct: 126 AGAIAGAIGAAVGNPADVAMVRMQADGRLPLTDRRNYKSVLDAITQMIRGEGVTSLWRGS 185

Query: 197 GPNIARNAIINAAELASYDQVKQTILKIPGFTDNVVTHLLAGLGAGFFAVCIGSPVDVVK 256
              I R  ++ +++LASYD VK+TIL+     D + TH+ A   AGF A    +PVDV+K
Sbjct: 186 SLTINRAMLVTSSQLASYDSVKETILEKGLLKDGLGTHVSASFAAGFVASVASNPVDVIK 245

Query: 257 SRMMGDST-------YKSTLDCFVKTLRNDGPLAFYKGFIPNFGRLGSWNVIMFLTLEQA 302
           +R+M           YK  +DC +KT++ +G ++ YKGFIP   R   + V++F+TLEQ 
Sbjct: 246 TRVMNMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTVSRQAPFTVVLFVTLEQV 305

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O818454.7e-14786.29Mitochondrial uncoupling protein 1 OS=Arabidopsis thaliana OX=3702 GN=PUMP1 PE=1... [more]
Q9ZWG16.4e-12872.52Mitochondrial uncoupling protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUMP2 PE=2... [more]
P565011.7e-7248.79Mitochondrial uncoupling protein 3 OS=Mus musculus OX=10090 GN=Ucp3 PE=1 SV=1[more]
P564995.0e-7248.79Mitochondrial uncoupling protein 3 OS=Rattus norvegicus OX=10116 GN=Ucp3 PE=2 SV... [more]
Q9N2I96.6e-7248.80Mitochondrial uncoupling protein 3 OS=Canis lupus familiaris OX=9615 GN=UCP3 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0KXV22.7e-169100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G307900 PE=3 SV=1[more]
A0A5A7TT481.3e-16899.34Mitochondrial uncoupling protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BLL81.3e-16899.34mitochondrial uncoupling protein 1 OS=Cucumis melo OX=3656 GN=LOC103490980 PE=3 ... [more]
A0A6J1IM654.4e-16495.72mitochondrial uncoupling protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478... [more]
A0A6J1GFM74.4e-16495.72mitochondrial uncoupling protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
XP_004147893.15.5e-169100.00mitochondrial uncoupling protein 1 [Cucumis sativus] >KGN54348.1 hypothetical pr... [more]
XP_008448974.12.8e-16899.34PREDICTED: mitochondrial uncoupling protein 1 [Cucumis melo] >KAA0045067.1 mitoc... [more]
XP_038880991.11.5e-16697.37mitochondrial uncoupling protein 1 [Benincasa hispida][more]
XP_022950708.19.2e-16495.72mitochondrial uncoupling protein 1-like [Cucurbita moschata] >XP_022978241.1 mit... [more]
XP_022966614.11.6e-16394.74mitochondrial uncoupling protein 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G54110.13.4e-14886.29plant uncoupling mitochondrial protein 1 [more]
AT5G58970.14.6e-12972.52uncoupling protein 2 [more]
AT5G58970.21.5e-10067.40uncoupling protein 2 [more]
AT4G24570.12.5e-5036.89dicarboxylate carrier 2 [more]
AT2G22500.15.5e-5035.39uncoupling protein 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002030Mitochondrial carrier UCP-likePRINTSPR00784MTUNCOUPLINGcoord: 44..63
score: 31.15
coord: 97..109
score: 30.18
coord: 18..30
score: 28.99
coord: 210..228
score: 41.7
coord: 167..184
score: 50.0
coord: 285..304
score: 29.37
IPR023395Mitochondrial carrier domain superfamilyGENE3D1.50.40.10Mitochondrial carrier domaincoord: 14..303
e-value: 1.0E-82
score: 279.3
IPR023395Mitochondrial carrier domain superfamilySUPERFAMILY103506Mitochondrial carriercoord: 10..292
IPR018108Mitochondrial substrate/solute carrierPFAMPF00153Mito_carrcoord: 10..105
e-value: 1.8E-18
score: 66.2
coord: 112..207
e-value: 2.1E-19
score: 69.2
coord: 214..298
e-value: 7.3E-19
score: 67.5
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 113..204
score: 27.082521
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 213..296
score: 27.254147
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 11..103
score: 26.5891
NoneNo IPR availablePANTHERPTHR45618:SF12MITOCHONDRIAL UNCOUPLING PROTEIN 1coord: 4..297
NoneNo IPR availablePANTHERPTHR45618MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATEDcoord: 4..297

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G15590.1CSPI04G15590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0003333 amino acid transmembrane transport
biological_process GO:1990542 mitochondrial transmembrane transport
biological_process GO:0006839 mitochondrial transport
biological_process GO:0009853 photorespiration
biological_process GO:1902600 proton transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005743 mitochondrial inner membrane
cellular_component GO:0031966 mitochondrial membrane
molecular_function GO:0015171 amino acid transmembrane transporter activity
molecular_function GO:0017077 oxidative phosphorylation uncoupler activity