CSPI03G42250 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G42250
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptioncytochrome P450 CYP82D47-like
LocationChr3: 36347995 .. 36351108 (+)
RNA-Seq ExpressionCSPI03G42250
SyntenyCSPI03G42250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTAAAAAGGAATCCAAAACCAATTACCTTTATTAGAGAAAACAACGAATTTTATAATCTCCAAAAATGGTGGAGTTTCAATTCCCCTTCTTTAACCCAAAATCCATCACCATGCTCGTCTTTTTGTTTTTCATTTCATGCTACTACCTGTGGCAGAAGTGGCAACAGTACAAATCTACTGAACCGAAACAAGCTCCAGCACCGCCGGGAGCATGGCCGATTATAGGCCACCTCCATATGCTCCACAATGTGAAGCTCCCCCATCATGCTCTTGGAGCCATGGCCGATAAATATGGGCCCCTCTTCCGGCTGCAGCTGGGGTCACGGTCGGCTTTGGTGGTGAGCAGCTGGGAAATGGCCAAGGAAAGCATGTGTGTTAACGACGCTGCTGCCGCCTCCCGGCCTGGCGTCTCCGGTACCAAACATTTTTCCTATGACTTCGCAGCATTCGGGTTGGCTCCTTACAGTCCTTACTGGAGGGAGATTCGTAAGGTTACTCACATGGAGCTCCTCTCTAATCCACGCGTTGATCAGGTTTTAACACTACAAATTATGCAAAATTTTATCTTCATGACACTCATCATATACCATATGTCCAAAGGTCCATGGATTATCACCAAAGAATCTATAAGACACAATGCTTGACTTCCAAATAGTTAATGTTGATTGATTTGATAAAAGTGAAAGGTAAACTATTGTAAATGATAAATTAGTAAAAGTATTTACAAAATACTGCAAATTTTTTTTATTGTATTTATATTGTAGAAACAAATTTTGATGATTTTTATCAAACCAATAATAATTAGATTTTAACATGTAAAACCATAATTATGATTTTTTTCTAAACCAACATATAAAAACAATCTTTGTAATTTAACCTTGTTATAACTTGAGGTACAAAAATTTATATTTTCATTTTTTGAATACCAAACTGAGTACTCCCATGCCTTCAGATTTGAAAAATAATTATGATAGGTCCACAGTTACATTTCGAGTTTTGGACCATTTTTGTGTTGATATTTGTAGTTTTTAGAAAAAGTTATATTTATATTTTATTTTTTAATTGAAAGATGCATTAAAATAAGAAATCTATACTCATACAAGAAAAAGTGTTTGATTAGGGTGTATTTAAAATGTTCTTACTAATATTACAAAACGACACAGTTTAAGAACACCATGTTTGGTGAGGTGAAGACATCATTAAGAGAGCTGCACGAAACATGGGCAGCACAGAAGGATGGATCGGGTCAGGTTGAAGTGGAGATGAAGCGTTGGTTTGGGGATGTGATAGTGAATATGCTTCTGAAGATCATCATCGGAAAACGATGTGTCGGTCCAAACGCCGAGGGAGGTGAAAAACAAGCTAAAGATTTTCAGTTGGCAATTAGGGACTCATTCCATTTGATGGGTCAGGGGTTGTTGAGAGATTATATTCCTCTGATTGGCCGGCTCGGATTTAATGGACAAGTGAAGGTGATGGAGAACATAGCCACACGATTCGACATGGTTCTAAGGGAATGGCTGGATGAACACAAACTTAATAGAACTTCTTCATGTTGTGGTAGAAAAGATGGAGATTTTATGGATGCTCTTGTTTCTCTATATGATGGCAAAGAGATTGAAGGTTACTATGATGGTGATACCATCATCAAAGCCACAACCCTCGTAAGTCTATCCTTCTTATTTTTCAAATTTTACTTCTAAACTTTGAGATCGGGGAGTTATTGATCATTTTTTAAAATTTATTATCAAGAATAAATGAATAATTATTTCTAAGTAAATGGAGAAAGATTGCTGAAAGCGAAAAGAAGGAAAAATTAATTATGTTAAGGACTATTATTTATTTGATTCAAACAATTTCTTTTCTCTATAATATGCCTGTTTAATATAATTATTGCTTGTTTGATATTCTATAAGTTTAAATAACCGTTTTGAAATTTTATTGGTACAATCAACTTTTTTTTCTTTACTTTTTCTTTTTAAATTTTCTTAAAGTTTTAGAGTAACTTGAAATAAAACTTGTTTTCATTTAAAACTGATAATTGACATAGAGTTTAAGTGTTTTTTTGTAATACTTTTGTTTAAGACAATATTTTGGAAACCTTTGAAAGTTGCATAATTAAAACTAGATTTAAAAGAAAAAAAGAAAAAAAGTAAAAGGGCATTCTTTTTATAATATAAGTTCTTTCAACATCAACATTAATACATATCCAAACAATACATGTTAAACTTTAGATATGCAATAAAATTTACTGTAACCCATCACTTAAATTTTTCGGTTAACGATAGATTTATCTAAGATTAAACTAAAAAATATTATTTGATAAAAATTTAGTCGTACGTATGTGCAGAATATGGTAGCCGGTGGAACTGAAAGCACGACAGTAACTCTAACATGGGCGATGTCATTGTTAATAAATAACCCACACGTCCTCGAAAGGGCACAGCAAGAGCTCGACACGGTGGTTGGCCGAGACAGACAACTGAAGGAGTCGGACATACCAAATCTTGTTTATTTAAAGTCCATAATCAAAGAGACTATGCGAATGTATCCAGCAGGGCCTTTATTAGGACCACGTGAGTTCTACAAGGACTGCATTGTAGCTGGCTACTTTGTCCCCAAAGGCACCCAATTGATCCCAAACATTTGGAAGATCCAAACAGATCCACGTGTATGGCCTGACCCGTTCGAGTTCAAGCCAGAGCGGTTCTTGACAACCCATAAGAATGTGGATTTGAAAGGGAATAATTTTGAGTTGATTCCATTTGGGAGTGGAAGGAGAGGTTGCCCTGGACTTGCTTTTGGACTTCAAATGGTGCATTTTGCTTTGGCTGGGTTTTTACATTCCTTTGATGTCAAAAACCCAACCAAAGAACCTATTGATATGTCAGAGAATTTTGGAATGGCTAATGAGAAAGTTGTTCCTCTCAATGTTTCGGTCACGTCACGGTTACCTTCTCATCTTTACACAATAAATTAATCAAGCTCTTCTCATGGTGAATTTTATATCGCCACTTCCTTTTATTTAACGTGTAATGTTTATCAGTTTATCTTAAATATATTGTTGCCACACAACAAAGGCTACATTTTGTGGTATACCGCTTAAGGGTCCT

mRNA sequence

CTTAAAAAGGAATCCAAAACCAATTACCTTTATTAGAGAAAACAACGAATTTTATAATCTCCAAAAATGGTGGAGTTTCAATTCCCCTTCTTTAACCCAAAATCCATCACCATGCTCGTCTTTTTGTTTTTCATTTCATGCTACTACCTGTGGCAGAAGTGGCAACAGTACAAATCTACTGAACCGAAACAAGCTCCAGCACCGCCGGGAGCATGGCCGATTATAGGCCACCTCCATATGCTCCACAATGTGAAGCTCCCCCATCATGCTCTTGGAGCCATGGCCGATAAATATGGGCCCCTCTTCCGGCTGCAGCTGGGGTCACGGTCGGCTTTGGTGGTGAGCAGCTGGGAAATGGCCAAGGAAAGCATGTGTGTTAACGACGCTGCTGCCGCCTCCCGGCCTGGCGTCTCCGGTACCAAACATTTTTCCTATGACTTCGCAGCATTCGGGTTGGCTCCTTACAGTCCTTACTGGAGGGAGATTCGTAAGGTTACTCACATGGAGCTCCTCTCTAATCCACGCGTTGATCAGTTTAAGAACACCATGTTTGGTGAGGTGAAGACATCATTAAGAGAGCTGCACGAAACATGGGCAGCACAGAAGGATGGATCGGGTCAGGTTGAAGTGGAGATGAAGCGTTGGTTTGGGGATGTGATAGTGAATATGCTTCTGAAGATCATCATCGGAAAACGATGTGTCGGTCCAAACGCCGAGGGAGGTGAAAAACAAGCTAAAGATTTTCAGTTGGCAATTAGGGACTCATTCCATTTGATGGGTCAGGGGTTGTTGAGAGATTATATTCCTCTGATTGGCCGGCTCGGATTTAATGGACAAGTGAAGGTGATGGAGAACATAGCCACACGATTCGACATGGTTCTAAGGGAATGGCTGGATGAACACAAACTTAATAGAACTTCTTCATGTTGTGGTAGAAAAGATGGAGATTTTATGGATGCTCTTGTTTCTCTATATGATGGCAAAGAGATTGAAGGTTACTATGATGGTGATACCATCATCAAAGCCACAACCCTCAATATGGTAGCCGGTGGAACTGAAAGCACGACAGTAACTCTAACATGGGCGATGTCATTGTTAATAAATAACCCACACGTCCTCGAAAGGGCACAGCAAGAGCTCGACACGGTGGTTGGCCGAGACAGACAACTGAAGGAGTCGGACATACCAAATCTTGTTTATTTAAAGTCCATAATCAAAGAGACTATGCGAATGTATCCAGCAGGGCCTTTATTAGGACCACGTGAGTTCTACAAGGACTGCATTGTAGCTGGCTACTTTGTCCCCAAAGGCACCCAATTGATCCCAAACATTTGGAAGATCCAAACAGATCCACGTGTATGGCCTGACCCGTTCGAGTTCAAGCCAGAGCGGTTCTTGACAACCCATAAGAATGTGGATTTGAAAGGGAATAATTTTGAGTTGATTCCATTTGGGAGTGGAAGGAGAGGTTGCCCTGGACTTGCTTTTGGACTTCAAATGGTGCATTTTGCTTTGGCTGGGTTTTTACATTCCTTTGATGTCAAAAACCCAACCAAAGAACCTATTGATATGTCAGAGAATTTTGGAATGGCTAATGAGAAAGTTGTTCCTCTCAATGTTTCGGTCACGTCACGGTTACCTTCTCATCTTTACACAATAAATTAATCAAGCTCTTCTCATGGTGAATTTTATATCGCCACTTCCTTTTATTTAACGTGTAATGTTTATCAGTTTATCTTAAATATATTGTTGCCACACAACAAAGGCTACATTTTGTGGTATACCGCTTAAGGGTCCT

Coding sequence (CDS)

ATGGTGGAGTTTCAATTCCCCTTCTTTAACCCAAAATCCATCACCATGCTCGTCTTTTTGTTTTTCATTTCATGCTACTACCTGTGGCAGAAGTGGCAACAGTACAAATCTACTGAACCGAAACAAGCTCCAGCACCGCCGGGAGCATGGCCGATTATAGGCCACCTCCATATGCTCCACAATGTGAAGCTCCCCCATCATGCTCTTGGAGCCATGGCCGATAAATATGGGCCCCTCTTCCGGCTGCAGCTGGGGTCACGGTCGGCTTTGGTGGTGAGCAGCTGGGAAATGGCCAAGGAAAGCATGTGTGTTAACGACGCTGCTGCCGCCTCCCGGCCTGGCGTCTCCGGTACCAAACATTTTTCCTATGACTTCGCAGCATTCGGGTTGGCTCCTTACAGTCCTTACTGGAGGGAGATTCGTAAGGTTACTCACATGGAGCTCCTCTCTAATCCACGCGTTGATCAGTTTAAGAACACCATGTTTGGTGAGGTGAAGACATCATTAAGAGAGCTGCACGAAACATGGGCAGCACAGAAGGATGGATCGGGTCAGGTTGAAGTGGAGATGAAGCGTTGGTTTGGGGATGTGATAGTGAATATGCTTCTGAAGATCATCATCGGAAAACGATGTGTCGGTCCAAACGCCGAGGGAGGTGAAAAACAAGCTAAAGATTTTCAGTTGGCAATTAGGGACTCATTCCATTTGATGGGTCAGGGGTTGTTGAGAGATTATATTCCTCTGATTGGCCGGCTCGGATTTAATGGACAAGTGAAGGTGATGGAGAACATAGCCACACGATTCGACATGGTTCTAAGGGAATGGCTGGATGAACACAAACTTAATAGAACTTCTTCATGTTGTGGTAGAAAAGATGGAGATTTTATGGATGCTCTTGTTTCTCTATATGATGGCAAAGAGATTGAAGGTTACTATGATGGTGATACCATCATCAAAGCCACAACCCTCAATATGGTAGCCGGTGGAACTGAAAGCACGACAGTAACTCTAACATGGGCGATGTCATTGTTAATAAATAACCCACACGTCCTCGAAAGGGCACAGCAAGAGCTCGACACGGTGGTTGGCCGAGACAGACAACTGAAGGAGTCGGACATACCAAATCTTGTTTATTTAAAGTCCATAATCAAAGAGACTATGCGAATGTATCCAGCAGGGCCTTTATTAGGACCACGTGAGTTCTACAAGGACTGCATTGTAGCTGGCTACTTTGTCCCCAAAGGCACCCAATTGATCCCAAACATTTGGAAGATCCAAACAGATCCACGTGTATGGCCTGACCCGTTCGAGTTCAAGCCAGAGCGGTTCTTGACAACCCATAAGAATGTGGATTTGAAAGGGAATAATTTTGAGTTGATTCCATTTGGGAGTGGAAGGAGAGGTTGCCCTGGACTTGCTTTTGGACTTCAAATGGTGCATTTTGCTTTGGCTGGGTTTTTACATTCCTTTGATGTCAAAAACCCAACCAAAGAACCTATTGATATGTCAGAGAATTTTGGAATGGCTAATGAGAAAGTTGTTCCTCTCAATGTTTCGGTCACGTCACGGTTACCTTCTCATCTTTACACAATAAATTAA

Protein sequence

MVEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLHNVKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKHFSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQKDGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGLLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALVSLYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDTVVGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHFALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN*
Homology
BLAST of CSPI03G42250 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 3.9e-144
Identity = 247/518 (47.68%), Postives = 344/518 (66.41%), Query Frame = 0

Query: 13  SITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLHNVKLPHHALGAM 72
           SI  L+FL    C +L++     K++  K AP   GAWPI+GHL +L+  + PH  LGA+
Sbjct: 17  SILSLIFL----CLFLYR-----KNSRGKDAPVVSGAWPILGHLSLLNGSQTPHKVLGAL 76

Query: 73  ADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKHFSYDFAAFGLAP 132
           ADKYGPLF ++LG + ALV+S+WEM+KE    ND A +SRP +   +  SY+ A  GLAP
Sbjct: 77  ADKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFVGLAP 136

Query: 133 YSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWA-AQKDGSGQVEVEMK 192
           Y PYWRE+RK+   E LSN R++Q  +    EV+TS++EL + W+   K+ S    V++K
Sbjct: 137 YGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLVDIK 196

Query: 193 RWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGLLRDYIPLIGR 252
           +W   +  NM++++++GKR  G     G+ +A+ F   IR+  +LMG   + D +P +  
Sbjct: 197 QWLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVPCLRW 256

Query: 253 LGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALVSLYDGKEIEGY 312
           L   G  K M+  A   D +L EWL+EH+  +        D DFMD ++S  +G +I G 
Sbjct: 257 LDLGGHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVESDRDFMDVMISALNGAQI-GA 316

Query: 313 YDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDTVVGRDRQLKES 372
           +D DTI KAT+L ++ GGT+ST VTLTWA+SLL+ NP  L +A++E+D  +G+D  ++ES
Sbjct: 317 FDADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEYIRES 376

Query: 373 DIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNIWKIQTDPRV 432
           DI  LVYL++I+KET+R+YP  P   PREF ++CI+ GY + KGT+LI N+WKI  DP V
Sbjct: 377 DISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHRDPSV 436

Query: 433 WPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHFALAGFLHSFD 492
           W DP EFKPERFLTTHK+VDL+G+NFEL+PFGSGRR C G++ GL MVHF LA  LHSFD
Sbjct: 437 WSDPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLANLLHSFD 496

Query: 493 VKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLY 530
           + NP+ EP+DM+E FG  N K  PL + V  R   + Y
Sbjct: 497 ILNPSAEPVDMTEFFGFTNTKATPLEILVKPRQSPNYY 524

BLAST of CSPI03G42250 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 5.8e-140
Identity = 247/528 (46.78%), Postives = 350/528 (66.29%), Query Frame = 0

Query: 2   VEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLHN 61
           +E    F N  +I  +V L F+ C +L+   ++   +  K+AP   GAWPI GHL +L  
Sbjct: 1   MELVLHFLNTTTIG-VVSLIFLLCLFLYGPLKKVHGSS-KEAPTVGGAWPIFGHLPLLIG 60

Query: 62  VKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKHF 121
            K PH ALGA+A+K+GPLF ++LG++ ALVVS WEMA+E    ND A ++RP +   +  
Sbjct: 61  SKSPHKALGALAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELM 120

Query: 122 SYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQKD 181
            Y+ A   +APY PYWRE+RK+   E+LS+ RV+Q ++    EV+ S+ EL++ W +QK+
Sbjct: 121 CYNNAMLLVAPYGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKN 180

Query: 182 GSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGL 241
            S    VE+K+WF   I NM+L++++GKR +  +A   +++A+    A+ +   L G   
Sbjct: 181 ESDYASVELKQWFAQPIFNMVLRMVVGKRFL--SATATDEKAEKCVKAVDEFMRLAGVFT 240

Query: 242 LRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALVS 301
           + D IP +  L F G  K M+  A   D+++ EWL+EH+  R          DFM+ ++S
Sbjct: 241 VGDAIPYLRWLDFGGYEKAMKETAKELDVMISEWLEEHRQKRALGEGVDGAQDFMNVMLS 300

Query: 302 LYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDTV 361
             DGK I+G  D DT+IK+T L ++  GTE++  T+ WAM L++ NP +LE  + ELD  
Sbjct: 301 SLDGKTIDG-IDADTLIKSTVLTIIQAGTEASISTIIWAMCLILKNPLILEN-KAELDIQ 360

Query: 362 VGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPN 421
           VG+DR + ESDI NLVYL++++KET+R+Y  GPL  PREF +DC + GY V KGT+LI N
Sbjct: 361 VGKDRCICESDISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLITN 420

Query: 422 IWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHF 481
           IWKI TDP VW DPFEFKP+RFLTTHK++D+KG++F+L+PFGSGRR CPG++FGLQ VH 
Sbjct: 421 IWKIHTDPNVWSDPFEFKPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTVHL 480

Query: 482 ALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLY 530
           ALA FLHSF++ NP+ EP+DM+E FG+ N K  PL V V   L    Y
Sbjct: 481 ALASFLHSFEILNPSTEPLDMTEAFGVTNTKATPLEVLVKPCLSPSCY 522

BLAST of CSPI03G42250 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 490.0 bits (1260), Expect = 3.5e-137
Identity = 239/543 (44.01%), Postives = 342/543 (62.98%), Query Frame = 0

Query: 10  NPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLHNVKLPHHAL 69
           N  + T + F+  IS  +   ++ +   T  K+ P   G+WP++GHL ++ N + PH  L
Sbjct: 4   NYLNTTTIAFISLISLLFFLFRFSKVSHT--KEPPIISGSWPLLGHLPLMRNTQTPHKTL 63

Query: 70  GAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKHFSYDFAAFG 129
           GA+ DKYGP+F ++LG+ +ALV+S+WE+AKE    ND   +SRP     +  SY+ A  G
Sbjct: 64  GALVDKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIG 123

Query: 130 LAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQ---------- 189
            APY  YWR++RK+  +E+LSN R++   +    EV+TS++EL   W+ Q          
Sbjct: 124 WAPYGAYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDD 183

Query: 190 ---------KDGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAI 249
                       +  V VE+K+WF  + +NM+L++++GKRC G      +++AK F   I
Sbjct: 184 TKSSSTNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLENI 243

Query: 250 RDSFHLMGQGLLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHK----LNRTSS 309
           RD   L+G   + D +P +  L   G  K M+  A +FD++L EWL+EH+    L     
Sbjct: 244 RDFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSEDK 303

Query: 310 CCGRKDGDFMDALVSLYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLIN 369
             G +  DFMDA++ +   K IEG +D DTIIKATTL ++ GG+++T  TLTWAM LL+ 
Sbjct: 304 VVGER--DFMDAMLLVLKDKPIEG-FDVDTIIKATTLELILGGSDTTAGTLTWAMCLLLK 363

Query: 370 NPHVLERAQQELDTVVGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCI 429
           +PHVLE+ ++EL+T +G++R + ESDI  LVYL +IIKET+R+YP  P   PREF +DC 
Sbjct: 364 HPHVLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCT 423

Query: 430 VAGYFVPKGTQLIPNIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGR 489
           + GY + KGT+L+PN+WKI  DP VWPDP EFKPERFL+THK+VD++G NFEL+PFGSGR
Sbjct: 424 IGGYHIKKGTRLMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLPFGSGR 483

Query: 490 RGCPGLAFGLQMVHFALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPS 530
           R C G++ GL MVH+ LA FLHSF++ NP+ E ID++E       K  PL V V   L  
Sbjct: 484 RMCAGMSLGLHMVHYILANFLHSFEILNPSPESIDVTEVLEFVTTKATPLEVLVKPCLSF 541

BLAST of CSPI03G42250 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 4.7e-134
Identity = 237/502 (47.21%), Postives = 330/502 (65.74%), Query Frame = 0

Query: 36  KSTEPK--QAPAPPGAWPIIGHLHML-HNVKLPHHALGAMADKYGPLFRLQLGSRSALVV 95
           KS +PK  +APAP GAWPIIGHLH+L    +L +  LG MAD YGP   LQLGS  A VV
Sbjct: 23  KSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLQLGSNEAFVV 82

Query: 96  SSWEMAKESMCVNDAAAASRPGVSGTKHFSYDFAAFGLAPYSPYWREIRKVTHMELLSNP 155
           SS+E+AK+   VND A ASRP  +  KH  Y+FA FG APYS +WRE+RK+  +ELLSN 
Sbjct: 83  SSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKIATIELLSNR 142

Query: 156 RVDQFKNTMFGEVKTSLRELHETWAAQKDGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCV 215
           R+   K+    E+   +++L+  W  +  G+  V V++K W  D+ +NM+++++ GKR  
Sbjct: 143 RLQMLKHVRVSEITMGVKDLYSLW-FKNGGTKPVMVDLKSWLEDMTLNMIVRMVAGKRYF 202

Query: 216 GPN---AEGGEKQAKDFQLAIRDSFHLMGQGLLRDYIPLIGRLGFNGQVKVMENIATRFD 275
           G     +    ++A   + AI   FHL+G   + D  P +      G  K M+   +  D
Sbjct: 203 GGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKEMKQTGSELD 262

Query: 276 MVLREWLDEHKLNRTSSCCGRKDGDFMDALVSLYD-GKEIEGYYDGDTIIKATTLNMVAG 335
           ++L  W++ H+  R  S     D DF+D ++SL + GK     YD +T IK+T L ++ G
Sbjct: 263 VILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSHLQYDANTSIKSTCLALILG 322

Query: 336 GTESTTVTLTWAMSLLINNPHVLERAQQELDTVVGRDRQLKESDIPNLVYLKSIIKETMR 395
           G++++  TLTWA+SLL+NN  +L++AQ E+D  VGRDR +++SDI NLVYL++IIKET+R
Sbjct: 323 GSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYLQAIIKETLR 382

Query: 396 MYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNIWKIQTDPRVWPDPFEFKPERFLTTH- 455
           +YPAGPLLGPRE  +DC VAGY+VP GT+LI N+WKIQ DP+V+ +P EF+PERF+T   
Sbjct: 383 LYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERFITGEA 442

Query: 456 KNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHFALAGFLHSFDVKNPTKEPIDMSENFG 515
           K  D++G NFEL+PFGSGRR CPG +  +Q++H  LA FLHSFDVK     P+DMSEN G
Sbjct: 443 KEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTVMDMPVDMSENPG 502

Query: 516 MANEKVVPLNVSVTSRLPSHLY 530
           +   K  PL V ++ R+   L+
Sbjct: 503 LTIPKATPLEVLISPRIKEELF 523

BLAST of CSPI03G42250 vs. ExPASy Swiss-Prot
Match: O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)

HSP 1 Score: 468.0 bits (1203), Expect = 1.4e-130
Identity = 232/501 (46.31%), Postives = 329/501 (65.67%), Query Frame = 0

Query: 36  KSTEPK--QAPAPPGAWPIIGHLHMLH-NVKLPHHALGAMADKYGPLFRLQLGSRSALVV 95
           KS +PK  +APAP GAWPIIGHLH+L    +L +  LG MAD+YGP   L+LGS    VV
Sbjct: 23  KSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGPAMSLRLGSSETFVV 82

Query: 96  SSWEMAKESMCVNDAAAASRPGVSGTKHFSYDFAAFGLAPYSPYWREIRKVTHMELLSNP 155
           SS+E+AK+   VND A ASRP  +  KH  YD A FG APYS +WRE+RK+  +ELLSN 
Sbjct: 83  SSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWREMRKIATLELLSNR 142

Query: 156 RVDQFKNTMFGEVKTSLRELHETWAAQKDGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCV 215
           R+   K+    E+   +++L+  W  +K GS  V V++K W  D+ +NM+++++ GKR  
Sbjct: 143 RLQMLKHVRVSEISMVMQDLYSLW-VKKGGSEPVMVDLKSWLEDMSLNMMVRMVAGKRYF 202

Query: 216 GPNAEGGE--KQAKDFQLAIRDSFHLMGQGLLRDYIPLIGRLGFNGQVKVMENIATRFDM 275
           G  +   E  ++A+  +  + + FHL+G   + D  P +G   F G  K M+      D+
Sbjct: 203 GGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGHEKEMKQTGRELDV 262

Query: 276 VLREWLDEHKLNRTSSCCGRKDGDFMDALVSLYD-GKEIEGYYDGDTIIKATTLNMVAGG 335
           +L  W++ H+  R  S     D DF+D ++SL + GK     +D  T IK+T L ++ GG
Sbjct: 263 ILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHLQHDAITSIKSTCLALILGG 322

Query: 336 TESTTVTLTWAMSLLINNPHVLERAQQELDTVVGRDRQLKESDIPNLVYLKSIIKETMRM 395
           +E++  TLTWA+SLL+NN  +L++AQ E+D  VGRDR +++SDI NLVY+++IIKET+R+
Sbjct: 323 SETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLVYIQAIIKETLRL 382

Query: 396 YPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNIWKIQTDPRVWPDPFEFKPERFLTTH-K 455
           YPAGPLLG RE  +DC VAGY V +GT+++ N+WKIQ DPRV+ +P EF+PERF+T   K
Sbjct: 383 YPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNEFRPERFITGEAK 442

Query: 456 NVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHFALAGFLHSFDVKNPTKEPIDMSENFGM 515
             D++G NFEL+PFGSGRR CPG +  +Q++H  LA FL SFDVK     P+DM+E+ G+
Sbjct: 443 EFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVMDMPVDMTESPGL 502

Query: 516 ANEKVVPLNVSVTSRLPSHLY 530
              K  PL + ++ RL   LY
Sbjct: 503 TIPKATPLEILISPRLKEGLY 522

BLAST of CSPI03G42250 vs. ExPASy TrEMBL
Match: A0A0A0LD43 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852590 PE=3 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 532/532 (100.00%), Postives = 532/532 (100.00%), Query Frame = 0

Query: 1   MVEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLH 60
           MVEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLH
Sbjct: 1   MVEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLH 60

Query: 61  NVKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKH 120
           NVKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKH
Sbjct: 61  NVKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKH 120

Query: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQK 180
           FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQK
Sbjct: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQK 180

Query: 181 DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG 240
           DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG
Sbjct: 181 DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG 240

Query: 241 LLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALV 300
           LLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALV
Sbjct: 241 LLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALV 300

Query: 301 SLYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDT 360
           SLYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDT
Sbjct: 301 SLYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDT 360

Query: 361 VVGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP 420
           VVGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP
Sbjct: 361 VVGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP 420

Query: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVH 480
           NIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVH
Sbjct: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVH 480

Query: 481 FALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN 533
           FALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN
Sbjct: 481 FALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN 532

BLAST of CSPI03G42250 vs. ExPASy TrEMBL
Match: A0A1S3CMR1 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502568 PE=3 SV=1)

HSP 1 Score: 1052.4 bits (2720), Expect = 6.5e-304
Identity = 501/532 (94.17%), Postives = 518/532 (97.37%), Query Frame = 0

Query: 1   MVEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLH 60
           M+EFQFPFFNPKSITML+  FFISCYYLW+KW+QYKST  KQAPAPPGA PIIGHLHMLH
Sbjct: 1   MIEFQFPFFNPKSITMLILWFFISCYYLWRKWKQYKSTNLKQAPAPPGALPIIGHLHMLH 60

Query: 61  NVKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKH 120
           NVKLPHHALGAMADKYGP+FRLQLGSRSALVVSSWEMAKESMCVNDAAAASRP VSGTKH
Sbjct: 61  NVKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPAVSGTKH 120

Query: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQK 180
           FSYDFAAFGLAPY PYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSL ELHETWAAQK
Sbjct: 121 FSYDFAAFGLAPYGPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLTELHETWAAQK 180

Query: 181 DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG 240
           DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNA+GGEKQAKDFQLAIRDSFHLMGQG
Sbjct: 181 DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAKGGEKQAKDFQLAIRDSFHLMGQG 240

Query: 241 LLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALV 300
           LLRDY+PLIG LGFNGQVKVMENIATRFD+VL EWLDEHK NRTSSCCGR+DGDFMDALV
Sbjct: 241 LLRDYVPLIGWLGFNGQVKVMENIATRFDVVLSEWLDEHKRNRTSSCCGRRDGDFMDALV 300

Query: 301 SLYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDT 360
           SLYDGK IEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLL+NNPHVLERAQQELDT
Sbjct: 301 SLYDGKGIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLLNNPHVLERAQQELDT 360

Query: 361 VVGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP 420
           VVGRDR+LKESDIPNLVYL+SIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP
Sbjct: 361 VVGRDRRLKESDIPNLVYLQSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP 420

Query: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVH 480
           N+W+IQTDPRVWPDPF+FKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPG AFGLQMVH
Sbjct: 421 NLWRIQTDPRVWPDPFKFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGAAFGLQMVH 480

Query: 481 FALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN 533
           FALAGFLHSFDV+NPTKEPIDMSENFGMANEKVVPLNV VTSRLPSHLYT+N
Sbjct: 481 FALAGFLHSFDVRNPTKEPIDMSENFGMANEKVVPLNVLVTSRLPSHLYTVN 532

BLAST of CSPI03G42250 vs. ExPASy TrEMBL
Match: A0A6J1FBI1 (cytochrome P450 82A3-like OS=Cucurbita moschata OX=3662 GN=LOC111442563 PE=3 SV=1)

HSP 1 Score: 923.3 bits (2385), Expect = 4.6e-265
Identity = 439/523 (83.94%), Postives = 479/523 (91.59%), Query Frame = 0

Query: 11  PKSITMLVFLFFI-SCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLHNVKLPHHAL 70
           P SIT+L+ LFFI SCYYLW+KWQQ+K  E KQAP+PPGAWPIIGHLH+LHN KLPHHAL
Sbjct: 6   PSSITVLLLLFFITSCYYLWRKWQQHKVAERKQAPSPPGAWPIIGHLHLLHNAKLPHHAL 65

Query: 71  GAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKHFSYDFAAFG 130
           GAMADKYGP+FRLQLGSRSALVVSSWEMAKESMC NDAAAASRPGVSGTK+FSYDFAAFG
Sbjct: 66  GAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCFNDAAAASRPGVSGTKYFSYDFAAFG 125

Query: 131 LAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQKDGSGQVEVE 190
           LAPYSPYWREIRKVTH+ELLSNPRV Q K+TM  ++ TSL ELHETW AQKD SGQV VE
Sbjct: 126 LAPYSPYWREIRKVTHIELLSNPRVHQVKSTMLAQINTSLTELHETWGAQKDESGQVAVE 185

Query: 191 MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGLLRDYIPLI 250
           MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEK+AKD QLAIRDSFHLMGQGLL+DYIPLI
Sbjct: 186 MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKEAKDIQLAIRDSFHLMGQGLLQDYIPLI 245

Query: 251 GRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALVSLYDGKEIE 310
           GRLGFNGQV VM+NIA RFDMVL EWL+EHK  R++S  G  D DFMDAL+SLY+GKEIE
Sbjct: 246 GRLGFNGQVPVMKNIAKRFDMVLSEWLEEHKRRRSAS-SGENDRDFMDALISLYNGKEIE 305

Query: 311 GYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDTVVGRDRQLK 370
           GYYDGDTI KATTLNM AGGTESTTVTLTWAMSLL+NNPHVLE+A QELD VVGR+R++ 
Sbjct: 306 GYYDGDTITKATTLNMFAGGTESTTVTLTWAMSLLLNNPHVLEKAWQELDAVVGRERRVT 365

Query: 371 ESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNIWKIQTDP 430
           E D+  LVYL++IIKETMR+YPAGPLLGPREF+KDC+V GYFVPKGTQLIPNIWKIQTDP
Sbjct: 366 EDDVGKLVYLQAIIKETMRLYPAGPLLGPREFHKDCVVGGYFVPKGTQLIPNIWKIQTDP 425

Query: 431 RVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHFALAGFLHS 490
           RVW +PFEFKPERFLTTHK+VDLKGN+FELIPFGSGRRGCPG+AFGLQM+HF LAGFL  
Sbjct: 426 RVWCEPFEFKPERFLTTHKDVDLKGNHFELIPFGSGRRGCPGVAFGLQMMHFTLAGFLQC 485

Query: 491 FDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN 533
           FDVKNPTK+PIDMSENFGMANEKV+PL+V VTSRLPSHL+  N
Sbjct: 486 FDVKNPTKDPIDMSENFGMANEKVLPLHVLVTSRLPSHLHAFN 527

BLAST of CSPI03G42250 vs. ExPASy TrEMBL
Match: A0A6J1IGS1 (cytochrome P450 82A3-like OS=Cucurbita maxima OX=3661 GN=LOC111476809 PE=3 SV=1)

HSP 1 Score: 916.0 bits (2366), Expect = 7.3e-263
Identity = 440/532 (82.71%), Postives = 481/532 (90.41%), Query Frame = 0

Query: 2   VEFQFPFFNPKSITMLVFLFFI-SCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLH 61
           +EFQ     P SI +L+ LFFI SCYYLW+KWQQ+K  + KQAP+PPGAWPIIGHLH+LH
Sbjct: 1   MEFQL----PSSIIVLLHLFFITSCYYLWRKWQQHKLADRKQAPSPPGAWPIIGHLHLLH 60

Query: 62  NVKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKH 121
           N KLPHHALGAMADKYGP+F LQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTK+
Sbjct: 61  NAKLPHHALGAMADKYGPIFSLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKY 120

Query: 122 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQK 181
           FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRV +  +TM  E+ TSL ELHETW AQK
Sbjct: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVYKVASTMLAEINTSLTELHETWGAQK 180

Query: 182 DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG 241
           D SGQV VEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEK+AKD QLAIRDSFHLMGQG
Sbjct: 181 DESGQVAVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKEAKDIQLAIRDSFHLMGQG 240

Query: 242 LLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALV 301
           LL+DYIPLIGRLGFNGQV VMENIA RFDMVL +WL+EHK  R++S  G KD DFMDAL+
Sbjct: 241 LLQDYIPLIGRLGFNGQVPVMENIAKRFDMVLSQWLEEHKRRRSAS-SGEKDRDFMDALI 300

Query: 302 SLYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDT 361
           SL++GK IEGYYDGDTI KATTLNM+AGGTESTTVTLTWAMSLL+NNPHVLE+A QELD 
Sbjct: 301 SLHNGKGIEGYYDGDTITKATTLNMIAGGTESTTVTLTWAMSLLLNNPHVLEKAWQELDA 360

Query: 362 VVGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP 421
           VVGR+R++ E DI  LVYL++IIKETMR+YPAGPLLGPREF+KDC+V GYFVPKGTQLIP
Sbjct: 361 VVGRERRVTEDDISKLVYLQAIIKETMRLYPAGPLLGPREFHKDCVVGGYFVPKGTQLIP 420

Query: 422 NIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVH 481
           NIWKIQTDPRVW +PFEFKPERFLTTHK+VDLKGN+FELIPFGSGRRGCP  AFGLQM+H
Sbjct: 421 NIWKIQTDPRVWCEPFEFKPERFLTTHKDVDLKGNHFELIPFGSGRRGCPAAAFGLQMMH 480

Query: 482 FALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN 533
           F LAGFL  FDVKNPTK+PIDMSENFGMANEKVVPL+V VTSRLPSHL+ +N
Sbjct: 481 FTLAGFLQCFDVKNPTKDPIDMSENFGMANEKVVPLHVLVTSRLPSHLHALN 527

BLAST of CSPI03G42250 vs. ExPASy TrEMBL
Match: A0A6J1DJE5 (cytochrome P450 82A3-like OS=Momordica charantia OX=3673 GN=LOC111021426 PE=3 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 6.9e-245
Identity = 409/532 (76.88%), Postives = 457/532 (85.90%), Query Frame = 0

Query: 1   MVEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLH 60
           ++E QFP FNP S+  LV +F    YYL +KWQ     E KQAP+PPGAWPIIGHLH+LH
Sbjct: 48  IMESQFPPFNPTSVATLVGIFVFCYYYLRRKWQ-----ERKQAPSPPGAWPIIGHLHLLH 107

Query: 61  NVKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKH 120
           N KLPHH LGAMADKYGP+FRLQLG RSALVVS+WEMAKESMC+NDAAA SRPG+SGTKH
Sbjct: 108 NAKLPHHTLGAMADKYGPIFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKH 167

Query: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQK 180
           FSYD+AAFGLAPYSPYWRE+RK+TH+ELLSN RVDQ KN MF ++  SL ELH       
Sbjct: 168 FSYDYAAFGLAPYSPYWREMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELHA------ 227

Query: 181 DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG 240
            GS Q+ VEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEK AKD Q+AIRDSFHLMGQG
Sbjct: 228 -GSAQIPVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQG 287

Query: 241 LLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALV 300
           LL DYIPLIGRLGF+GQVKVME+IA RFD+++ EWL+EHK  R       +DGDFMD+L+
Sbjct: 288 LLTDYIPLIGRLGFDGQVKVMESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLI 347

Query: 301 SLYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDT 360
           SL DGK I+GYYD DTIIKATTLNM+AGGTESTTVTLTW M+LL+NNPH LE AQQELD 
Sbjct: 348 SLLDGKGIQGYYDADTIIKATTLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDA 407

Query: 361 VVGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP 420
           VVGRDR++ ESDI  L YL++++KE MRMYPAGPLLGPREF  DC+VAGYFVP+GTQLIP
Sbjct: 408 VVGRDRRVNESDISKLHYLQAVVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIP 467

Query: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVH 480
           NIWKIQTDPRVWP+PFEFKPERFLTTHK+VD KGN+FELIPFGSGRRGCPG+AFGLQMVH
Sbjct: 468 NIWKIQTDPRVWPNPFEFKPERFLTTHKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVH 527

Query: 481 FALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN 533
           F LAGFL SFDVK+ + + IDMSENFGMANEKVVPLNV VTSRLPSHL+  N
Sbjct: 528 FTLAGFLQSFDVKSSSADLIDMSENFGMANEKVVPLNVLVTSRLPSHLHGFN 567

BLAST of CSPI03G42250 vs. NCBI nr
Match: XP_004147824.1 (cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59910.1 hypothetical protein Csa_000849 [Cucumis sativus])

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 532/532 (100.00%), Postives = 532/532 (100.00%), Query Frame = 0

Query: 1   MVEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLH 60
           MVEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLH
Sbjct: 1   MVEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLH 60

Query: 61  NVKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKH 120
           NVKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKH
Sbjct: 61  NVKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKH 120

Query: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQK 180
           FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQK
Sbjct: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQK 180

Query: 181 DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG 240
           DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG
Sbjct: 181 DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG 240

Query: 241 LLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALV 300
           LLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALV
Sbjct: 241 LLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALV 300

Query: 301 SLYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDT 360
           SLYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDT
Sbjct: 301 SLYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDT 360

Query: 361 VVGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP 420
           VVGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP
Sbjct: 361 VVGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP 420

Query: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVH 480
           NIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVH
Sbjct: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVH 480

Query: 481 FALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN 533
           FALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN
Sbjct: 481 FALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN 532

BLAST of CSPI03G42250 vs. NCBI nr
Match: XP_008464762.1 (PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo])

HSP 1 Score: 1052.4 bits (2720), Expect = 1.3e-303
Identity = 501/532 (94.17%), Postives = 518/532 (97.37%), Query Frame = 0

Query: 1   MVEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLH 60
           M+EFQFPFFNPKSITML+  FFISCYYLW+KW+QYKST  KQAPAPPGA PIIGHLHMLH
Sbjct: 1   MIEFQFPFFNPKSITMLILWFFISCYYLWRKWKQYKSTNLKQAPAPPGALPIIGHLHMLH 60

Query: 61  NVKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKH 120
           NVKLPHHALGAMADKYGP+FRLQLGSRSALVVSSWEMAKESMCVNDAAAASRP VSGTKH
Sbjct: 61  NVKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPAVSGTKH 120

Query: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQK 180
           FSYDFAAFGLAPY PYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSL ELHETWAAQK
Sbjct: 121 FSYDFAAFGLAPYGPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLTELHETWAAQK 180

Query: 181 DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG 240
           DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNA+GGEKQAKDFQLAIRDSFHLMGQG
Sbjct: 181 DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAKGGEKQAKDFQLAIRDSFHLMGQG 240

Query: 241 LLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALV 300
           LLRDY+PLIG LGFNGQVKVMENIATRFD+VL EWLDEHK NRTSSCCGR+DGDFMDALV
Sbjct: 241 LLRDYVPLIGWLGFNGQVKVMENIATRFDVVLSEWLDEHKRNRTSSCCGRRDGDFMDALV 300

Query: 301 SLYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDT 360
           SLYDGK IEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLL+NNPHVLERAQQELDT
Sbjct: 301 SLYDGKGIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLLNNPHVLERAQQELDT 360

Query: 361 VVGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP 420
           VVGRDR+LKESDIPNLVYL+SIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP
Sbjct: 361 VVGRDRRLKESDIPNLVYLQSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP 420

Query: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVH 480
           N+W+IQTDPRVWPDPF+FKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPG AFGLQMVH
Sbjct: 421 NLWRIQTDPRVWPDPFKFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGAAFGLQMVH 480

Query: 481 FALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN 533
           FALAGFLHSFDV+NPTKEPIDMSENFGMANEKVVPLNV VTSRLPSHLYT+N
Sbjct: 481 FALAGFLHSFDVRNPTKEPIDMSENFGMANEKVVPLNVLVTSRLPSHLYTVN 532

BLAST of CSPI03G42250 vs. NCBI nr
Match: XP_038897047.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 1001.5 bits (2588), Expect = 2.7e-288
Identity = 476/532 (89.47%), Postives = 505/532 (94.92%), Query Frame = 0

Query: 1   MVEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLH 60
           M+EFQFP FNP+SI ML  LFFISCYYLW+K +Q+  +E KQAP  PGAWPIIGHLH++H
Sbjct: 1   MMEFQFPSFNPESIAMLFVLFFISCYYLWRKLEQHNVSERKQAPVLPGAWPIIGHLHLMH 60

Query: 61  NVKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKH 120
           N KLPHHALGAMADKYGP+FRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKH
Sbjct: 61  NAKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKH 120

Query: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQK 180
           FSYDFAAFGLAPYSPYWREIRKVTHMELLSN RVDQFKNTMFGE+KTSL ELH+TWAAQK
Sbjct: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNQRVDQFKNTMFGEIKTSLTELHKTWAAQK 180

Query: 181 DGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG 240
           D SGQV+VEMKRWFGDVI+NMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG
Sbjct: 181 DESGQVQVEMKRWFGDVILNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG 240

Query: 241 LLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALV 300
           LLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWL+EHK  R SS  G+K+ DFMDAL+
Sbjct: 241 LLRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLEEHKRKRASSSSGKKNEDFMDALL 300

Query: 301 SLYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDT 360
           SLYDGK IEGYYDGDTIIKATTLNM+AGGTESTTVTLTWAMSLL+NNPHVLE+AQQELDT
Sbjct: 301 SLYDGKRIEGYYDGDTIIKATTLNMIAGGTESTTVTLTWAMSLLLNNPHVLEKAQQELDT 360

Query: 361 VVGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIP 420
           VVGRDR+LK+SDIPNLVYL  IIKETMR+YPAGPLLGPREFYKDCIV GYFVPKGTQLIP
Sbjct: 361 VVGRDRRLKDSDIPNLVYLHCIIKETMRLYPAGPLLGPREFYKDCIVDGYFVPKGTQLIP 420

Query: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVH 480
           NIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFG+GRRGCPG++FGLQMVH
Sbjct: 421 NIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGTGRRGCPGISFGLQMVH 480

Query: 481 FALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN 533
           FALAGFLHSFDV+ PTKEPIDMSENFGMANEKVVPLNV VTSRLPS+LY +N
Sbjct: 481 FALAGFLHSFDVRKPTKEPIDMSENFGMANEKVVPLNVLVTSRLPSYLYALN 532

BLAST of CSPI03G42250 vs. NCBI nr
Match: XP_022935740.1 (cytochrome P450 82A3-like [Cucurbita moschata])

HSP 1 Score: 923.3 bits (2385), Expect = 9.4e-265
Identity = 439/523 (83.94%), Postives = 479/523 (91.59%), Query Frame = 0

Query: 11  PKSITMLVFLFFI-SCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLHNVKLPHHAL 70
           P SIT+L+ LFFI SCYYLW+KWQQ+K  E KQAP+PPGAWPIIGHLH+LHN KLPHHAL
Sbjct: 6   PSSITVLLLLFFITSCYYLWRKWQQHKVAERKQAPSPPGAWPIIGHLHLLHNAKLPHHAL 65

Query: 71  GAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKHFSYDFAAFG 130
           GAMADKYGP+FRLQLGSRSALVVSSWEMAKESMC NDAAAASRPGVSGTK+FSYDFAAFG
Sbjct: 66  GAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCFNDAAAASRPGVSGTKYFSYDFAAFG 125

Query: 131 LAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQKDGSGQVEVE 190
           LAPYSPYWREIRKVTH+ELLSNPRV Q K+TM  ++ TSL ELHETW AQKD SGQV VE
Sbjct: 126 LAPYSPYWREIRKVTHIELLSNPRVHQVKSTMLAQINTSLTELHETWGAQKDESGQVAVE 185

Query: 191 MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGLLRDYIPLI 250
           MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEK+AKD QLAIRDSFHLMGQGLL+DYIPLI
Sbjct: 186 MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKEAKDIQLAIRDSFHLMGQGLLQDYIPLI 245

Query: 251 GRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALVSLYDGKEIE 310
           GRLGFNGQV VM+NIA RFDMVL EWL+EHK  R++S  G  D DFMDAL+SLY+GKEIE
Sbjct: 246 GRLGFNGQVPVMKNIAKRFDMVLSEWLEEHKRRRSAS-SGENDRDFMDALISLYNGKEIE 305

Query: 311 GYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDTVVGRDRQLK 370
           GYYDGDTI KATTLNM AGGTESTTVTLTWAMSLL+NNPHVLE+A QELD VVGR+R++ 
Sbjct: 306 GYYDGDTITKATTLNMFAGGTESTTVTLTWAMSLLLNNPHVLEKAWQELDAVVGRERRVT 365

Query: 371 ESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNIWKIQTDP 430
           E D+  LVYL++IIKETMR+YPAGPLLGPREF+KDC+V GYFVPKGTQLIPNIWKIQTDP
Sbjct: 366 EDDVGKLVYLQAIIKETMRLYPAGPLLGPREFHKDCVVGGYFVPKGTQLIPNIWKIQTDP 425

Query: 431 RVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHFALAGFLHS 490
           RVW +PFEFKPERFLTTHK+VDLKGN+FELIPFGSGRRGCPG+AFGLQM+HF LAGFL  
Sbjct: 426 RVWCEPFEFKPERFLTTHKDVDLKGNHFELIPFGSGRRGCPGVAFGLQMMHFTLAGFLQC 485

Query: 491 FDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN 533
           FDVKNPTK+PIDMSENFGMANEKV+PL+V VTSRLPSHL+  N
Sbjct: 486 FDVKNPTKDPIDMSENFGMANEKVLPLHVLVTSRLPSHLHAFN 527

BLAST of CSPI03G42250 vs. NCBI nr
Match: XP_023535455.1 (cytochrome P450 82A3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 920.2 bits (2377), Expect = 8.0e-264
Identity = 437/523 (83.56%), Postives = 478/523 (91.40%), Query Frame = 0

Query: 11  PKSITMLVFLFFI-SCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLHNVKLPHHAL 70
           P SIT+L+ LFFI SCYYLW+KWQ +K  E KQAP+PPGAWPIIGHLH+LHN KLPHHAL
Sbjct: 6   PSSITVLLHLFFITSCYYLWRKWQHHKVAERKQAPSPPGAWPIIGHLHLLHNAKLPHHAL 65

Query: 71  GAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKHFSYDFAAFG 130
           GAMADKYGP+FRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTK+FSYDFAAFG
Sbjct: 66  GAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYFSYDFAAFG 125

Query: 131 LAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQKDGSGQVEVE 190
           LAPYSPYWREIRKVTHMELLSNPRV Q K+ M  ++ TSL ELHETW AQKD SGQV VE
Sbjct: 126 LAPYSPYWREIRKVTHMELLSNPRVHQVKSPMLAQINTSLTELHETWGAQKDESGQVAVE 185

Query: 191 MKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGLLRDYIPLI 250
           MKRWFGDVI+NMLLKIIIGKRCVGPNAEGGEK+AKD QLAIRDSFHLMGQGLL+DYIPLI
Sbjct: 186 MKRWFGDVIMNMLLKIIIGKRCVGPNAEGGEKEAKDIQLAIRDSFHLMGQGLLQDYIPLI 245

Query: 251 GRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALVSLYDGKEIE 310
           GRLGFNGQV VMENIA RFDMVL +WL+EHK  R++S  G KD DFMDAL+SLY+GKEIE
Sbjct: 246 GRLGFNGQVPVMENIAKRFDMVLSQWLEEHKRRRSAS-SGEKDRDFMDALISLYNGKEIE 305

Query: 311 GYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDTVVGRDRQLK 370
           GYYDGD I KATTLNM+AGGTESTTVTLTWAMSLL+NNPHVLE+A QELD VVGR+R++ 
Sbjct: 306 GYYDGDIITKATTLNMIAGGTESTTVTLTWAMSLLLNNPHVLEKAWQELDAVVGRERRVT 365

Query: 371 ESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNIWKIQTDP 430
           E DI  LVYL++IIKETMR+YPAGPLLGPREF+KDC+V GYFVPKGTQLIPNIWKIQTDP
Sbjct: 366 EDDISKLVYLQAIIKETMRLYPAGPLLGPREFHKDCVVGGYFVPKGTQLIPNIWKIQTDP 425

Query: 431 RVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHFALAGFLHS 490
           RVW +PFEFKPERF+ THK+VDLKGN+FELIPFGSGRRGCPG+AFGLQM+HF LAGFL  
Sbjct: 426 RVWCEPFEFKPERFMRTHKDVDLKGNHFELIPFGSGRRGCPGVAFGLQMMHFTLAGFLQC 485

Query: 491 FDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN 533
           FDVKNPTK+PIDMSENFGMANEKV+PL+V VTSRLPSHL+  N
Sbjct: 486 FDVKNPTKDPIDMSENFGMANEKVLPLHVLVTSRLPSHLHAFN 527

BLAST of CSPI03G42250 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 479.6 bits (1233), Expect = 3.4e-135
Identity = 237/502 (47.21%), Postives = 330/502 (65.74%), Query Frame = 0

Query: 36  KSTEPK--QAPAPPGAWPIIGHLHML-HNVKLPHHALGAMADKYGPLFRLQLGSRSALVV 95
           KS +PK  +APAP GAWPIIGHLH+L    +L +  LG MAD YGP   LQLGS  A VV
Sbjct: 23  KSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLQLGSNEAFVV 82

Query: 96  SSWEMAKESMCVNDAAAASRPGVSGTKHFSYDFAAFGLAPYSPYWREIRKVTHMELLSNP 155
           SS+E+AK+   VND A ASRP  +  KH  Y+FA FG APYS +WRE+RK+  +ELLSN 
Sbjct: 83  SSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKIATIELLSNR 142

Query: 156 RVDQFKNTMFGEVKTSLRELHETWAAQKDGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCV 215
           R+   K+    E+   +++L+  W  +  G+  V V++K W  D+ +NM+++++ GKR  
Sbjct: 143 RLQMLKHVRVSEITMGVKDLYSLW-FKNGGTKPVMVDLKSWLEDMTLNMIVRMVAGKRYF 202

Query: 216 GPN---AEGGEKQAKDFQLAIRDSFHLMGQGLLRDYIPLIGRLGFNGQVKVMENIATRFD 275
           G     +    ++A   + AI   FHL+G   + D  P +      G  K M+   +  D
Sbjct: 203 GGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKEMKQTGSELD 262

Query: 276 MVLREWLDEHKLNRTSSCCGRKDGDFMDALVSLYD-GKEIEGYYDGDTIIKATTLNMVAG 335
           ++L  W++ H+  R  S     D DF+D ++SL + GK     YD +T IK+T L ++ G
Sbjct: 263 VILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSHLQYDANTSIKSTCLALILG 322

Query: 336 GTESTTVTLTWAMSLLINNPHVLERAQQELDTVVGRDRQLKESDIPNLVYLKSIIKETMR 395
           G++++  TLTWA+SLL+NN  +L++AQ E+D  VGRDR +++SDI NLVYL++IIKET+R
Sbjct: 323 GSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYLQAIIKETLR 382

Query: 396 MYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNIWKIQTDPRVWPDPFEFKPERFLTTH- 455
           +YPAGPLLGPRE  +DC VAGY+VP GT+LI N+WKIQ DP+V+ +P EF+PERF+T   
Sbjct: 383 LYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERFITGEA 442

Query: 456 KNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHFALAGFLHSFDVKNPTKEPIDMSENFG 515
           K  D++G NFEL+PFGSGRR CPG +  +Q++H  LA FLHSFDVK     P+DMSEN G
Sbjct: 443 KEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTVMDMPVDMSENPG 502

Query: 516 MANEKVVPLNVSVTSRLPSHLY 530
           +   K  PL V ++ R+   L+
Sbjct: 503 LTIPKATPLEVLISPRIKEELF 523

BLAST of CSPI03G42250 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 468.0 bits (1203), Expect = 1.0e-131
Identity = 232/501 (46.31%), Postives = 329/501 (65.67%), Query Frame = 0

Query: 36  KSTEPK--QAPAPPGAWPIIGHLHMLH-NVKLPHHALGAMADKYGPLFRLQLGSRSALVV 95
           KS +PK  +APAP GAWPIIGHLH+L    +L +  LG MAD+YGP   L+LGS    VV
Sbjct: 23  KSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGPAMSLRLGSSETFVV 82

Query: 96  SSWEMAKESMCVNDAAAASRPGVSGTKHFSYDFAAFGLAPYSPYWREIRKVTHMELLSNP 155
           SS+E+AK+   VND A ASRP  +  KH  YD A FG APYS +WRE+RK+  +ELLSN 
Sbjct: 83  SSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWREMRKIATLELLSNR 142

Query: 156 RVDQFKNTMFGEVKTSLRELHETWAAQKDGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCV 215
           R+   K+    E+   +++L+  W  +K GS  V V++K W  D+ +NM+++++ GKR  
Sbjct: 143 RLQMLKHVRVSEISMVMQDLYSLW-VKKGGSEPVMVDLKSWLEDMSLNMMVRMVAGKRYF 202

Query: 216 GPNAEGGE--KQAKDFQLAIRDSFHLMGQGLLRDYIPLIGRLGFNGQVKVMENIATRFDM 275
           G  +   E  ++A+  +  + + FHL+G   + D  P +G   F G  K M+      D+
Sbjct: 203 GGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGHEKEMKQTGRELDV 262

Query: 276 VLREWLDEHKLNRTSSCCGRKDGDFMDALVSLYD-GKEIEGYYDGDTIIKATTLNMVAGG 335
           +L  W++ H+  R  S     D DF+D ++SL + GK     +D  T IK+T L ++ GG
Sbjct: 263 ILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHLQHDAITSIKSTCLALILGG 322

Query: 336 TESTTVTLTWAMSLLINNPHVLERAQQELDTVVGRDRQLKESDIPNLVYLKSIIKETMRM 395
           +E++  TLTWA+SLL+NN  +L++AQ E+D  VGRDR +++SDI NLVY+++IIKET+R+
Sbjct: 323 SETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLVYIQAIIKETLRL 382

Query: 396 YPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNIWKIQTDPRVWPDPFEFKPERFLTTH-K 455
           YPAGPLLG RE  +DC VAGY V +GT+++ N+WKIQ DPRV+ +P EF+PERF+T   K
Sbjct: 383 YPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNEFRPERFITGEAK 442

Query: 456 NVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHFALAGFLHSFDVKNPTKEPIDMSENFGM 515
             D++G NFEL+PFGSGRR CPG +  +Q++H  LA FL SFDVK     P+DM+E+ G+
Sbjct: 443 EFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVMDMPVDMTESPGL 502

Query: 516 ANEKVVPLNVSVTSRLPSHLY 530
              K  PL + ++ RL   LY
Sbjct: 503 TIPKATPLEILISPRLKEGLY 522

BLAST of CSPI03G42250 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 437.2 bits (1123), Expect = 1.9e-122
Identity = 229/502 (45.62%), Postives = 315/502 (62.75%), Query Frame = 0

Query: 36  KSTEPK--QAPAPPGAWPIIGHLHML-HNVKLPHHALGAMADKYGPLFRLQLGSRSALVV 95
           KS +PK  +APAP GAWPIIGHLH+L    +L +  LG MAD YGP   L+LGS    V 
Sbjct: 23  KSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLRLGSSETFVG 82

Query: 96  SSWEMAKESMCVNDAAAASRPGVSGTKHFSYDFAAFGLAPYSPYWREIRKVTHMELLSNP 155
           SS+E+AK+   VND A AS    +  KH  Y F           W E+RK+  +ELLSN 
Sbjct: 83  SSFEVAKDCFTVNDKALASLM-TAAAKHMGYVF-----------WLEMRKIAMIELLSNR 142

Query: 156 RVDQFKNTMFGEVKTSLRELHETWAAQKDGSGQVEVEMKRWFGDVIVNMLLKIIIGKRCV 215
           R+    N    E+   +++L+  W  +K GS  V V++K W  D+I NM+++++ GKR  
Sbjct: 143 RLQMLNNVRVSEISMGVKDLYSLW-VKKGGSEPVMVDLKSWLEDMIANMIMRMVAGKRYF 202

Query: 216 -GPNAEGGE--KQAKDFQLAIRDSFHLMGQGLLRDYIPLIGRLGFNGQVKVMENIATRFD 275
            G  AE  E  ++A+ ++  I   FHL+G   + D  P +G L   G  K M+      D
Sbjct: 203 GGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWLDLQGHEKEMKQTRRELD 262

Query: 276 MVLREWLDEHKLNRTSSCCGRKDGDFMDALVSLYD-GKEIEGYYDGDTIIKATTLNMVAG 335
           ++L  W++ H+  R  S     D DF+D ++SL + GK     YD +T IK T L ++ G
Sbjct: 263 VILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKLSHLQYDANTCIKTTCLALILG 322

Query: 336 GTESTTVTLTWAMSLLINNPHVLERAQQELDTVVGRDRQLKESDIPNLVYLKSIIKETMR 395
           G+E++  TLTWA+SLL+NN  +L++ Q E+D  VGRDR +++SDI NLVYL++IIKET+R
Sbjct: 323 GSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDSDIKNLVYLQAIIKETLR 382

Query: 396 MYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNIWKIQTDPRVWPDPFEFKPERFLTTH- 455
           +YPA PLLG RE  +DC VAGY VP GT+LI N+WKIQ DP+V+ +P EF+PERF+T   
Sbjct: 383 LYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERFITGEA 442

Query: 456 KNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHFALAGFLHSFDVKNPTKEPIDMSENFG 515
           K+ D++G NFEL+PFGSGRR CPG +  +QM+H  LA FLHSF+VK     P+DMSE+ G
Sbjct: 443 KDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFEVKTVLDRPVDMSESPG 502

Query: 516 MANEKVVPLNVSVTSRLPSHLY 530
           +   K  PL V +  RL   L+
Sbjct: 503 LTITKATPLEVLINPRLKRELF 511

BLAST of CSPI03G42250 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 391.3 bits (1004), Expect = 1.2e-108
Identity = 206/523 (39.39%), Postives = 306/523 (58.51%), Query Frame = 0

Query: 17  LVFLFFISCYYLWQKWQQYKS-TEPKQ----APAPPGAWPIIGHLHMLHNVKLPHHALGA 76
           L+ LF +S  +++      KS   PK     AP  PGAWP++GHLH+   V   H   GA
Sbjct: 3   LIMLFLLSALFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGA 62

Query: 77  MADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKHFSYDFAAFGLA 136
           MAD YGP+F  +LGS   ++++S E+AKE   V+D     RP ++ +K   Y+ +    +
Sbjct: 63  MADVYGPVFMAKLGSIKVMIINSKEVAKEIYTVHD-KLLERPELTASKLLGYNDSFLTFS 122

Query: 137 PYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQKDGSGQVEVEMK 196
           PY  YWREIRK+   EL S   VD    +   E   + R L+  W  +      V V+MK
Sbjct: 123 PYGLYWREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDMK 182

Query: 197 RWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGLLRDYIPLIGR 256
           + F D+  N+ L ++ GKR  G N     K+A+     IR+        LL D  P++G 
Sbjct: 183 QEFIDLTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGF 242

Query: 257 LGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALVSLYDGKEIEGY 316
           L +  + + M+  A   D V   W++EHK  R+    GR + D++D L+ +    +I G 
Sbjct: 243 LDWKTK-RGMKRTAKGLDKVAEGWIEEHKNKRSDH--GRSENDYLDILIKILGQDKIPGL 302

Query: 317 YDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDTVVGRDRQLKES 376
            D  T IKA  LN+V  G+E+  V L WA+SLL+NNPHVL +AQ+ELD+ +G++R ++E 
Sbjct: 303 SDTHTKIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEEL 362

Query: 377 DIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVA--GYFVPKGTQLIPNIWKIQTDP 436
           DI +LVYL++I+KET R+YP  PL+  R   +D  +A     VP GTQL+ + WKI  DP
Sbjct: 363 DIKDLVYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDP 422

Query: 437 RVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHFALAGFLHS 496
            VW +P +F+PERFLT+++ +D+ G +++  PFG GRR CP +  G++MVH+ L  FLHS
Sbjct: 423 NVWSNPEQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHS 482

Query: 497 FDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTIN 533
           FD+  P+ + +DM+E+ G+ N K  PL V++  RL   LY ++
Sbjct: 483 FDLARPSSQDVDMTESNGLVNHKATPLEVNIIPRLHKSLYEVD 521

BLAST of CSPI03G42250 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 361.3 bits (926), Expect = 1.3e-99
Identity = 199/525 (37.90%), Postives = 296/525 (56.38%), Query Frame = 0

Query: 9   FNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLHNVKLPHHA 68
           F+   +++      I  Y   +K       +    P P GA P+ GHLH+L   KL    
Sbjct: 5   FSTLQLSLFSLALVIFGYIFLRKQLSRCEVDSSTIPEPLGALPLFGHLHLLRGKKLLCKK 64

Query: 69  LGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKHFSYDFAAF 128
           L AM+ K+GP+F L+LG    +V S  +  K+    ND A A+RP ++  ++  Y+ A+ 
Sbjct: 65  LAAMSQKHGPIFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASL 124

Query: 129 GLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQKDGSGQVEV 188
            LAPY  YWRE+RK+  + L SN  ++   +    EV T ++ L+      K   G   V
Sbjct: 125 TLAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLY------KGNGGTSIV 184

Query: 189 EMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGLLRDYIPL 248
           ++   F  +  N++L+ ++GKR         E + K+   A++   +L    ++ D IP 
Sbjct: 185 KIDMLFEFLTFNIILRKMVGKRIGFGEVNSDEWRYKE---ALKHCEYLAVIPMIGDVIPW 244

Query: 249 IGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGD--FMDALVSLYDGK 308
           +G L F  +   M+ +    D V  +WL EH   R+ +    KD +   MD L+ +    
Sbjct: 245 LGWLDF-AKNSQMKRLFKELDSVNTKWLHEHLKKRSRN---EKDQERTIMDLLLDILPED 304

Query: 309 EIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDTVVGRDR 368
            +   +  D I+KAT L +   G++ST++TLTWA+SLL+NNP  LE AQ+E+D  VG+ R
Sbjct: 305 IVISGHVRDVIVKATILALTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGKGR 364

Query: 369 QLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNIWKIQ 428
            ++ESDI NL YL++I+KET R+YP  PL G RE  +DC V GY V KGT+L+ NIWK+ 
Sbjct: 365 WIEESDIQNLKYLQAIVKETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLH 424

Query: 429 TDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHFALAGF 488
            DP++WPDP  FKPERF+      +   +NFE IPFGSGRR CPG+  GL++VHF LA  
Sbjct: 425 RDPKIWPDPKTFKPERFMEDKSQCE--KSNFEYIPFGSGRRSCPGVNLGLRVVHFVLARL 484

Query: 489 LHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTI 532
           L  F++   + EP+DM+E  G+A  K+ P+ V V  RL   LY++
Sbjct: 485 LQGFELHKVSDEPLDMAEGPGLALPKINPVEVVVMPRLDPKLYSL 514

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O498583.9e-14447.68Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1[more]
O498595.8e-14046.78Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1[more]
Q430683.5e-13744.01Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2[more]
Q9SZ464.7e-13447.21Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=... [more]
O493941.4e-13046.31Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0LD430.0e+00100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852590 PE=3 SV=1[more]
A0A1S3CMR16.5e-30494.17cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502568 PE=3 SV=1[more]
A0A6J1FBI14.6e-26583.94cytochrome P450 82A3-like OS=Cucurbita moschata OX=3662 GN=LOC111442563 PE=3 SV=... [more]
A0A6J1IGS17.3e-26382.71cytochrome P450 82A3-like OS=Cucurbita maxima OX=3661 GN=LOC111476809 PE=3 SV=1[more]
A0A6J1DJE56.9e-24576.88cytochrome P450 82A3-like OS=Momordica charantia OX=3673 GN=LOC111021426 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_004147824.10.0e+00100.00cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59910.1 hypothetical protein Csa_... [more]
XP_008464762.11.3e-30394.17PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo][more]
XP_038897047.12.7e-28889.47cytochrome P450 CYP82D47-like [Benincasa hispida][more]
XP_022935740.19.4e-26583.94cytochrome P450 82A3-like [Cucurbita moschata][more]
XP_023535455.18.0e-26483.56cytochrome P450 82A3-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G31940.13.4e-13547.21cytochrome P450, family 82, subfamily C, polypeptide 4 [more]
AT4G31970.11.0e-13146.31cytochrome P450, family 82, subfamily C, polypeptide 2 [more]
AT4G31950.11.9e-12245.62cytochrome P450, family 82, subfamily C, polypeptide 3 [more]
AT2G25160.11.2e-10839.39cytochrome P450, family 82, subfamily F, polypeptide 1 [more]
AT3G25180.11.3e-9937.90cytochrome P450, family 82, subfamily G, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 381..392
score: 36.31
coord: 460..469
score: 57.4
coord: 328..345
score: 34.1
IPR001128Cytochrome P450PFAMPF00067p450coord: 44..509
e-value: 6.2E-89
score: 298.9
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 380..398
score: 31.32
coord: 73..92
score: 36.1
coord: 459..469
score: 53.79
coord: 421..445
score: 41.12
coord: 197..215
score: 17.09
coord: 469..492
score: 23.47
coord: 317..334
score: 24.13
coord: 337..363
score: 39.3
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 33..527
e-value: 1.6E-113
score: 381.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 44..523
NoneNo IPR availablePANTHERPTHR47947:SF18CYTOCHROME P450 82A2coord: 13..529
NoneNo IPR availablePANTHERPTHR47947CYTOCHROME P450 82C3-RELATEDcoord: 13..529
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 462..471

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G42250.1CSPI03G42250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098542 defense response to other organism
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen