CSPI03G24900 (gene) Cucumber (PI 183967) v1

BLAST of CSPI03G24900 vs. ExPASy Swiss-Prot
Match: P74003 (Release factor glutamine methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=prmC PE=3 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.3e-46
Identity = 107/280 (38.21%), Postives = 158/280 (56.43%), Query Frame = 0

Query: 80  ELKWLVQDAVEDKSLSSELENEIEQNPELGLRNVRLKMGIEELYRLWKQRIHERRPFQYI 139
           EL WL+Q   +   L+  L+       +   R + L+   E + R W++R+ E+ P QY+
Sbjct: 31  ELNWLLQGWTDLDRLTLRLQ-------DFAHREIALQETWENIQRGWRRRVEEKYPVQYL 90

Query: 140 VGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEALREG----LWVDLGTGSGAI 199
           +G   WRD ++ V + VLIPRPETE+++D+V+    ++ AL        WVDLGTGSGAI
Sbjct: 91  LGQTQWRDFVIKVTDDVLIPRPETELIIDIVQ---HEHSALSPSNCADHWVDLGTGSGAI 150

Query: 200 AIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQGSWYEPLQDVQGKLSG 259
           A+G+      +  V A D S  ALA+A  N Q     D I+  QG W+EPL+ ++G++ G
Sbjct: 151 ALGLAATFP-QALVHAVDCSGSALAIARENAQLNQFGDRIQFHQGYWWEPLEHLKGQVQG 210

Query: 260 IISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCDEATVMLKPGGFFAFE- 319
           ++SNPPYIP   +  LQ EV KHEP +ALDGG +G+  +  L   +   LKPGGF+  E 
Sbjct: 211 MVSNPPYIPQRELAQLQPEVIKHEPLLALDGGPDGLQAVEQLIRRSPTYLKPGGFWLVEI 270

Query: 320 -TNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFIT 354
            T        L+        G + +++I  D ASI RF++
Sbjct: 271 MTGQAPMVAELL-----RASGAYQDIQIHRDLASIERFVS 294

BLAST of CSPI03G24900 vs. ExPASy Swiss-Prot
Match: Q8DHV7 (Release factor glutamine methyltransferase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=prmC PE=3 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 6.8e-44
Identity = 91/202 (45.05%), Postives = 128/202 (63.37%), Query Frame = 0

Query: 113 VRLKMGIEELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEK 172
           + LK+ + EL   W++R  ER P QY++G  HW DL L V   VLIPRPETE L+ +V  
Sbjct: 53  IHLKLPLTELQERWQRRWQERVPLQYLIGVAHWHDLELVVTPSVLIPRPETEELLAVVAA 112

Query: 173 VVSDNEALREGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQ 232
            V   +  ++G W+DLGTGSGAIAIG+ R+      + A D SS AL VA  N+Q+Y L 
Sbjct: 113 TVPPWQ--QQGHWLDLGTGSGAIAIGLARLFPA-ALIHAVDCSSEALEVAQVNIQKYALG 172

Query: 233 DLIELRQGSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMD 292
           D +    G+W++P+  +QG++ GI+SNPPYIP+  +  LQ EV  HEP +ALDGGT+G+ 
Sbjct: 173 DRVRCYVGNWFDPIVPLQGQVQGIVSNPPYIPTSVVATLQPEVQYHEPLLALDGGTDGLQ 232

Query: 293 ELIHLCDEATVMLKPGGFFAFE 315
            +  + + A   L+P G+   E
Sbjct: 233 AIRQILETAPEYLQPQGWLFIE 251

BLAST of CSPI03G24900 vs. ExPASy Swiss-Prot
Match: Q7NJS7 (Release factor glutamine methyltransferase OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=prmC PE=3 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 7.6e-43
Identity = 103/255 (40.39%), Postives = 142/255 (55.69%), Query Frame = 0

Query: 109 GLRNVRLKMG--------IEELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPR 168
           GL  +R+++G         E+L  LW++RI E  P QY++G  HWRDL L V   VLIPR
Sbjct: 35  GLDRLRVRLGGPQALEAHREKLAALWRRRIEEAMPLQYLLGTAHWRDLQLQVNPAVLIPR 94

Query: 169 PETEVLVDLVEKVVSDNEALREGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALA 228
           PE+E LVD+           R    VDLGTGSGAIA+ + R L G   V A D S  AL 
Sbjct: 95  PESEALVDVAVDFCRSCAGARV---VDLGTGSGAIAVAVARALPG-ATVWAVDASEAALV 154

Query: 229 VAGYNVQRYGLQDLIELRQGSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEP 288
           VAG N++RYGL + + L +G+W+ PL         ++SNPPYIPS  I  L  EV  HEP
Sbjct: 155 VAGANIERYGLSEQVHLLRGNWFVPLP--TQPFDAVLSNPPYIPSAEIAALMPEVRLHEP 214

Query: 289 RVALDGGTNGMDELIHLCDEATVMLKPGGFFAFETNGEDQCKHLVNYMENNHKGKFCNLK 348
             ALDGG++G+D +  +  +A   L+PGG  A E     Q   +V  +  +   ++  ++
Sbjct: 215 LSALDGGSDGLDAVRQIIADAARHLRPGGILALEVMA-GQGPTVVQLLARD--SRYGCIR 274

Query: 349 IVSDFASIPRFITGF 356
            V D+A I R +  +
Sbjct: 275 TVRDWAGIERIVVTY 280

BLAST of CSPI03G24900 vs. ExPASy Swiss-Prot
Match: Q7VDL7 (Release factor glutamine methyltransferase OS=Prochlorococcus marinus (strain SARG / CCMP1375 / SS120) OX=167539 GN=prmC PE=3 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.2e-34
Identity = 87/241 (36.10%), Postives = 128/241 (53.11%), Query Frame = 0

Query: 114 RLKMGIEELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKV 173
           +L + +EEL  +W + + ++ P Q++VG   WRD  L V    LIPR ETE+L+D+  K 
Sbjct: 56  KLDLSLEELSLIWMRHLSDQIPLQHLVGKCPWRDFELKVNSSALIPRQETEILIDIALKK 115

Query: 174 VSDNEALREGLWVDLGTGSGAIAIGICRIL---EGRGRVIATDLSSIALAVAGYNVQRYG 233
           V D   ++ G W DLGTGSGA+A+ + R L   EG     A D  + ALA+A  N+    
Sbjct: 116 V-DAGLMKYGRWADLGTGSGALAVALARALPLWEGH----AADCCNDALALAESNINTLT 175

Query: 234 LQDLIELRQGSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNG 293
               + L  G W+EPL+   G    +++NPPYIP  ++  L   V  HEP +AL GG +G
Sbjct: 176 ENANVSLHLGDWWEPLKPWWGNFDLVVANPPYIPKTHLSELDPVVRDHEPILALSGGDDG 235

Query: 294 MDELIHLCDEATVMLKPGGFFAFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPR 352
           MD    +   A   L+ GG+   E N  DQ +  +N M ++    F  +   +D   + R
Sbjct: 236 MDSCRKVIKGAMKGLRSGGWLLLEHN-FDQSEQALNLMVDS---GFLEVDFENDLEGVRR 287

BLAST of CSPI03G24900 vs. ExPASy Swiss-Prot
Match: Q2RFW1 (Release factor glutamine methyltransferase OS=Moorella thermoacetica (strain ATCC 39073 / JCM 9320) OX=264732 GN=prmC PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.6e-29
Identity = 89/230 (38.70%), Postives = 127/230 (55.22%), Query Frame = 0

Query: 124 RLWKQRIHERR---PFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEAL 183
           R W Q I  R    P QY+ G + +  L   V   VLIPR +TEV+V+ V + +   E+ 
Sbjct: 56  RFW-QAIDRRAAGYPLQYLTGHQEFMSLDFKVTPAVLIPRQDTEVVVEAVLERLDPCESY 115

Query: 184 REGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQG 243
                 D GTGSGAIA+ +   L  R RV ATD+S  AL VA  N ++ GL   + L QG
Sbjct: 116 ---TIADCGTGSGAIALSLAHYLP-RARVYATDISPAALTVAQENARKLGLAARVTLLQG 175

Query: 244 SWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCDE 303
            +  PL+ +  KL  +++NPPYIP+  + GL A+V + EPR+ALDGG +G+D    L   
Sbjct: 176 DFLAPLRGL--KLDALVANPPYIPTAALPGLPADV-RSEPRLALDGGPDGLDAYRFLLPG 235

Query: 304 ATVMLKPGGFFAFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPR 351
           A  +L+PGG  A E  G DQ + + +       G + N +++ D+A   R
Sbjct: 236 AAGLLRPGGLLALEI-GSDQGQAVKDLA--RAVGAYRNEQVLPDYAGRDR 274

BLAST of CSPI03G24900 vs. ExPASy TrEMBL
Match: A0A0A0L8I9 (MTS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G524590 PE=4 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 1.0e-207
Identity = 357/360 (99.17%), Postives = 358/360 (99.44%), Query Frame = 0

Query: 1   MRLSIARAYLVAPHRAKSIRPICSSSLNPQIPLFLRPPNYSVTLKDFHKWHNWAKILNCS 60
           MRLSIARAYLVAPHRAKSIRPICSSSLNPQIPLFLRPPNYSVTLKDFHKWHNWAKILNCS
Sbjct: 1   MRLSIARAYLVAPHRAKSIRPICSSSLNPQIPLFLRPPNYSVTLKDFHKWHNWAKILNCS 60

Query: 61  VGSSFVDTDNGPDSTLLHRELKWLVQDAVEDKSLSSELENEIEQNPELGLRNVRLKMGIE 120
           VGSSFVDTDNGPDSTLLHRELKWLVQDAVEDKSLSSELENEIEQNPELGLRNVRLK+GIE
Sbjct: 61  VGSSFVDTDNGPDSTLLHRELKWLVQDAVEDKSLSSELENEIEQNPELGLRNVRLKVGIE 120

Query: 121 ELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEAL 180
           ELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEAL
Sbjct: 121 ELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEAL 180

Query: 181 REGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQG 240
           REGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQG
Sbjct: 181 REGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQG 240

Query: 241 SWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCDE 300
           SWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCDE
Sbjct: 241 SWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCDE 300

Query: 301 ATVMLKPGGFFAFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFITGFLNGAI 360
           ATVMLK GGF AFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFITGFLNGAI
Sbjct: 301 ATVMLKLGGFLAFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFITGFLNGAI 360

BLAST of CSPI03G24900 vs. ExPASy TrEMBL
Match: A0A5A7UDM4 (Release factor glutamine methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold113G001190 PE=4 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 7.8e-200
Identity = 345/361 (95.57%), Postives = 352/361 (97.51%), Query Frame = 0

Query: 1   MRLSIARAYLVAPHRAKSIRPICSSSL-NPQIPLFLRPPNYSVTLKDFHKWHNWAKILNC 60
           MRLSIARAYLVAPHRAKSIRPICSSS  NPQIPLFLRPPNYSVTL D HKWHNWAK LN 
Sbjct: 1   MRLSIARAYLVAPHRAKSIRPICSSSSPNPQIPLFLRPPNYSVTLTDVHKWHNWAKTLNS 60

Query: 61  SVGSSFVDTDNGPDSTLLHRELKWLVQDAVEDKSLSSELENEIEQNPELGLRNVRLKMGI 120
           SVGSSFV TDNGPDSTLLHRELKWL+QDAV+DKSLSSELENEIEQ PELGLRNVRLK+GI
Sbjct: 61  SVGSSFVHTDNGPDSTLLHRELKWLIQDAVDDKSLSSELENEIEQYPELGLRNVRLKVGI 120

Query: 121 EELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEA 180
           +ELYRLWKQRIH+RRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEA
Sbjct: 121 DELYRLWKQRIHDRRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEA 180

Query: 181 LREGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQ 240
           LREGLWVDLGTGSGAIAIGICRILEGRGRVIATDLS+IALAVAGYNVQRYGLQDLIELRQ
Sbjct: 181 LREGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSTIALAVAGYNVQRYGLQDLIELRQ 240

Query: 241 GSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCD 300
           GSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCD
Sbjct: 241 GSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCD 300

Query: 301 EATVMLKPGGFFAFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFITGFLNGA 360
           EA VMLKPGGFFAFETNGEDQCKHLVNYMEN+HKGKFCNLKIVSDFASIPRFITGFLNGA
Sbjct: 301 EAAVMLKPGGFFAFETNGEDQCKHLVNYMENSHKGKFCNLKIVSDFASIPRFITGFLNGA 360

BLAST of CSPI03G24900 vs. ExPASy TrEMBL
Match: A0A1S3BA23 (release factor glutamine methyltransferase OS=Cucumis melo OX=3656 GN=LOC103487646 PE=4 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 7.8e-200
Identity = 345/361 (95.57%), Postives = 352/361 (97.51%), Query Frame = 0

Query: 1   MRLSIARAYLVAPHRAKSIRPICSSSL-NPQIPLFLRPPNYSVTLKDFHKWHNWAKILNC 60
           MRLSIARAYLVAPHRAKSIRPICSSS  NPQIPLFLRPPNYSVTL D HKWHNWAK LN 
Sbjct: 1   MRLSIARAYLVAPHRAKSIRPICSSSSPNPQIPLFLRPPNYSVTLTDVHKWHNWAKTLNS 60

Query: 61  SVGSSFVDTDNGPDSTLLHRELKWLVQDAVEDKSLSSELENEIEQNPELGLRNVRLKMGI 120
           SVGSSFV TDNGPDSTLLHRELKWL+QDAV+DKSLSSELENEIEQ PELGLRNVRLK+GI
Sbjct: 61  SVGSSFVHTDNGPDSTLLHRELKWLIQDAVDDKSLSSELENEIEQYPELGLRNVRLKVGI 120

Query: 121 EELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEA 180
           +ELYRLWKQRIH+RRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEA
Sbjct: 121 DELYRLWKQRIHDRRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEA 180

Query: 181 LREGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQ 240
           LREGLWVDLGTGSGAIAIGICRILEGRGRVIATDLS+IALAVAGYNVQRYGLQDLIELRQ
Sbjct: 181 LREGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSTIALAVAGYNVQRYGLQDLIELRQ 240

Query: 241 GSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCD 300
           GSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCD
Sbjct: 241 GSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCD 300

Query: 301 EATVMLKPGGFFAFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFITGFLNGA 360
           EA VMLKPGGFFAFETNGEDQCKHLVNYMEN+HKGKFCNLKIVSDFASIPRFITGFLNGA
Sbjct: 301 EAAVMLKPGGFFAFETNGEDQCKHLVNYMENSHKGKFCNLKIVSDFASIPRFITGFLNGA 360

BLAST of CSPI03G24900 vs. ExPASy TrEMBL
Match: A0A6J1F974 (uncharacterized protein LOC111443257 OS=Cucurbita moschata OX=3662 GN=LOC111443257 PE=4 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 3.5e-176
Identity = 303/357 (84.87%), Postives = 320/357 (89.64%), Query Frame = 0

Query: 1   MRLSIARAYLVAPHRAKSIRPICSSSLNPQIPLFLRPPNYSVTLKDFHKWHNWAKILNCS 60
           MRLSI+RAYL AP R +  R ICS+S  P+IPLFLRPPNYS TL D  KWH+WAK L+ S
Sbjct: 16  MRLSISRAYLAAPRRVRPSRSICSASSKPRIPLFLRPPNYSATLTDLQKWHDWAKTLSSS 75

Query: 61  VGSSFVDTDNGPDSTLLHRELKWLVQDAVEDKSLSSELENEIEQNPELGLRNVRLKMGIE 120
           VGSSFVD DNGPDS LLHRELKWL++DAVEDKS+SSEL    EQN E   RNVRLK+GIE
Sbjct: 76  VGSSFVDADNGPDSALLHRELKWLIEDAVEDKSVSSELRTNSEQNHEHNHRNVRLKVGIE 135

Query: 121 ELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEAL 180
           ELY +WKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLV +VVSDNEAL
Sbjct: 136 ELYGVWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVAEVVSDNEAL 195

Query: 181 REGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQG 240
           REGLWVDLGTGSGAIAI ICR+LEGR RVIATDLS  AL VAGYNVQRYGLQD IELRQG
Sbjct: 196 REGLWVDLGTGSGAIAIAICRVLEGRARVIATDLSPTALTVAGYNVQRYGLQDSIELRQG 255

Query: 241 SWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCDE 300
           SWYEPL+DVQGKLSGIISNPPYIPSDNI GLQAEVGKHEPRVALDGGTNGMDELIHLCDE
Sbjct: 256 SWYEPLKDVQGKLSGIISNPPYIPSDNIVGLQAEVGKHEPRVALDGGTNGMDELIHLCDE 315

Query: 301 ATVMLKPGGFFAFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFITGFLN 358
           A VMLKPGGFFAFETNGEDQCKHLV YME+NH+G+ CNLKIVSDFA IPRFITGFLN
Sbjct: 316 AAVMLKPGGFFAFETNGEDQCKHLVKYMEDNHRGRLCNLKIVSDFAGIPRFITGFLN 372

BLAST of CSPI03G24900 vs. ExPASy TrEMBL
Match: A0A6J1IGQ1 (uncharacterized protein LOC111477071 OS=Cucurbita maxima OX=3661 GN=LOC111477071 PE=4 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 5.6e-174
Identity = 302/357 (84.59%), Postives = 321/357 (89.92%), Query Frame = 0

Query: 1   MRLSIARAYLVAPHRAKSIRPICSSSLNPQIPLFLRPPNYSVTLKDFHKWHNWAKILNCS 60
           MRLSI+RAYL APHR +  R ICSSS  P+IPLFLRPPNYS TL D  KWH+WAK L+ S
Sbjct: 17  MRLSISRAYLAAPHRVRPSRSICSSSSKPRIPLFLRPPNYSATLTDLQKWHDWAKTLSSS 76

Query: 61  VGSSFVDTDNGPDSTLLHRELKWLVQDAVEDKSLSSELENEIEQNPELGLRNVRLKMGIE 120
           VGSSFVD DNGPDS LLHRELKWLV+DAVEDKS+SSEL    EQN E  LRNVRLK+GIE
Sbjct: 77  VGSSFVDADNGPDSALLHRELKWLVEDAVEDKSISSELRTNSEQNHEHNLRNVRLKVGIE 136

Query: 121 ELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEAL 180
           ELYRLWKQRIHERRPFQYIVGCEHWRDLILSV EGVLIPRPETEVLVDLV +VVSDNEAL
Sbjct: 137 ELYRLWKQRIHERRPFQYIVGCEHWRDLILSV-EGVLIPRPETEVLVDLVAEVVSDNEAL 196

Query: 181 REGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQG 240
           REG WVDLGTGSGAIAI ICR+LEGRGRVIATDLS  AL VAGYNVQRYGLQ+LIELRQG
Sbjct: 197 REGFWVDLGTGSGAIAIAICRVLEGRGRVIATDLSPTALTVAGYNVQRYGLQELIELRQG 256

Query: 241 SWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCDE 300
           SWY+PL+DV GKLSGIISNPPYIPSDNI GLQAEVGKHEPRVALDGGTNGMDELIHLCDE
Sbjct: 257 SWYKPLRDVVGKLSGIISNPPYIPSDNIVGLQAEVGKHEPRVALDGGTNGMDELIHLCDE 316

Query: 301 ATVMLKPGGFFAFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFITGFLN 358
           A VMLKPGGFFAFETNGEDQ K+LV Y+E+NH+G+ C+LKIVSDFA IPRFITGFLN
Sbjct: 317 AAVMLKPGGFFAFETNGEDQSKYLVKYVEDNHRGRLCDLKIVSDFAGIPRFITGFLN 372

BLAST of CSPI03G24900 vs. NCBI nr
Match: XP_004151022.1 (uncharacterized protein LOC101217233 [Cucumis sativus] >KAE8652022.1 hypothetical protein Csa_004745 [Cucumis sativus])

HSP 1 Score: 732.3 bits (1889), Expect = 2.1e-207
Identity = 357/360 (99.17%), Postives = 358/360 (99.44%), Query Frame = 0

Query: 1   MRLSIARAYLVAPHRAKSIRPICSSSLNPQIPLFLRPPNYSVTLKDFHKWHNWAKILNCS 60
           MRLSIARAYLVAPHRAKSIRPICSSSLNPQIPLFLRPPNYSVTLKDFHKWHNWAKILNCS
Sbjct: 1   MRLSIARAYLVAPHRAKSIRPICSSSLNPQIPLFLRPPNYSVTLKDFHKWHNWAKILNCS 60

Query: 61  VGSSFVDTDNGPDSTLLHRELKWLVQDAVEDKSLSSELENEIEQNPELGLRNVRLKMGIE 120
           VGSSFVDTDNGPDSTLLHRELKWLVQDAVEDKSLSSELENEIEQNPELGLRNVRLK+GIE
Sbjct: 61  VGSSFVDTDNGPDSTLLHRELKWLVQDAVEDKSLSSELENEIEQNPELGLRNVRLKVGIE 120

Query: 121 ELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEAL 180
           ELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEAL
Sbjct: 121 ELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEAL 180

Query: 181 REGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQG 240
           REGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQG
Sbjct: 181 REGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQG 240

Query: 241 SWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCDE 300
           SWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCDE
Sbjct: 241 SWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCDE 300

Query: 301 ATVMLKPGGFFAFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFITGFLNGAI 360
           ATVMLK GGF AFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFITGFLNGAI
Sbjct: 301 ATVMLKLGGFLAFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFITGFLNGAI 360

BLAST of CSPI03G24900 vs. NCBI nr
Match: XP_008444272.1 (PREDICTED: release factor glutamine methyltransferase [Cucumis melo] >KAA0052296.1 release factor glutamine methyltransferase [Cucumis melo var. makuwa] >TYK01861.1 release factor glutamine methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 706.1 bits (1821), Expect = 1.6e-199
Identity = 345/361 (95.57%), Postives = 352/361 (97.51%), Query Frame = 0

Query: 1   MRLSIARAYLVAPHRAKSIRPICSSSL-NPQIPLFLRPPNYSVTLKDFHKWHNWAKILNC 60
           MRLSIARAYLVAPHRAKSIRPICSSS  NPQIPLFLRPPNYSVTL D HKWHNWAK LN 
Sbjct: 1   MRLSIARAYLVAPHRAKSIRPICSSSSPNPQIPLFLRPPNYSVTLTDVHKWHNWAKTLNS 60

Query: 61  SVGSSFVDTDNGPDSTLLHRELKWLVQDAVEDKSLSSELENEIEQNPELGLRNVRLKMGI 120
           SVGSSFV TDNGPDSTLLHRELKWL+QDAV+DKSLSSELENEIEQ PELGLRNVRLK+GI
Sbjct: 61  SVGSSFVHTDNGPDSTLLHRELKWLIQDAVDDKSLSSELENEIEQYPELGLRNVRLKVGI 120

Query: 121 EELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEA 180
           +ELYRLWKQRIH+RRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEA
Sbjct: 121 DELYRLWKQRIHDRRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEA 180

Query: 181 LREGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQ 240
           LREGLWVDLGTGSGAIAIGICRILEGRGRVIATDLS+IALAVAGYNVQRYGLQDLIELRQ
Sbjct: 181 LREGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSTIALAVAGYNVQRYGLQDLIELRQ 240

Query: 241 GSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCD 300
           GSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCD
Sbjct: 241 GSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCD 300

Query: 301 EATVMLKPGGFFAFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFITGFLNGA 360
           EA VMLKPGGFFAFETNGEDQCKHLVNYMEN+HKGKFCNLKIVSDFASIPRFITGFLNGA
Sbjct: 301 EAAVMLKPGGFFAFETNGEDQCKHLVNYMENSHKGKFCNLKIVSDFASIPRFITGFLNGA 360

BLAST of CSPI03G24900 vs. NCBI nr
Match: XP_038897020.1 (release factor glutamine methyltransferase [Benincasa hispida])

HSP 1 Score: 669.8 bits (1727), Expect = 1.3e-188
Identity = 326/360 (90.56%), Postives = 336/360 (93.33%), Query Frame = 0

Query: 1   MRLSIARAYLVAPHRAKSIRPICSSSLNPQIPLFLRPPNYSVTLKDFHKWHNWAKILNCS 60
           MRLSI+RAYLVAP   KS RPICSSS  PQIPLFLRPPNYSVTL D HKWHNWAK LN S
Sbjct: 1   MRLSISRAYLVAPICFKSTRPICSSSSKPQIPLFLRPPNYSVTLTDLHKWHNWAKTLNSS 60

Query: 61  VGSSFVDTDNGPDSTLLHRELKWLVQDAVEDKSLSSELENEIEQNPELGLRNVRLKMGIE 120
           VGSSFV+TDNGPDSTLLHRELKWLV+DA EDKSL  EL NEI QNP+LGLRNVRLK+GIE
Sbjct: 61  VGSSFVNTDNGPDSTLLHRELKWLVEDAAEDKSLIYELANEINQNPQLGLRNVRLKVGIE 120

Query: 121 ELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEAL 180
           ELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLV +VVSDNEAL
Sbjct: 121 ELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVGEVVSDNEAL 180

Query: 181 REGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQG 240
           REG WVDLGTGSGAIAI ICRILEGR RVIATDLSSIALAVAGYNVQRYGLQ+LIELRQG
Sbjct: 181 REGFWVDLGTGSGAIAIAICRILEGRARVIATDLSSIALAVAGYNVQRYGLQELIELRQG 240

Query: 241 SWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCDE 300
           SWY PLQD+QGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCDE
Sbjct: 241 SWYVPLQDLQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCDE 300

Query: 301 ATVMLKPGGFFAFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFITGFLNGAI 360
           A VMLKPGGFFAFETNGEDQCKHLVNYMENNHKG+FCNLKIVSDF  IPRFITGFLN A+
Sbjct: 301 AAVMLKPGGFFAFETNGEDQCKHLVNYMENNHKGRFCNLKIVSDFVDIPRFITGFLNEAM 360

BLAST of CSPI03G24900 vs. NCBI nr
Match: XP_022936763.1 (uncharacterized protein LOC111443257 [Cucurbita moschata] >XP_022936764.1 uncharacterized protein LOC111443257 [Cucurbita moschata])

HSP 1 Score: 627.5 bits (1617), Expect = 7.2e-176
Identity = 303/357 (84.87%), Postives = 320/357 (89.64%), Query Frame = 0

Query: 1   MRLSIARAYLVAPHRAKSIRPICSSSLNPQIPLFLRPPNYSVTLKDFHKWHNWAKILNCS 60
           MRLSI+RAYL AP R +  R ICS+S  P+IPLFLRPPNYS TL D  KWH+WAK L+ S
Sbjct: 16  MRLSISRAYLAAPRRVRPSRSICSASSKPRIPLFLRPPNYSATLTDLQKWHDWAKTLSSS 75

Query: 61  VGSSFVDTDNGPDSTLLHRELKWLVQDAVEDKSLSSELENEIEQNPELGLRNVRLKMGIE 120
           VGSSFVD DNGPDS LLHRELKWL++DAVEDKS+SSEL    EQN E   RNVRLK+GIE
Sbjct: 76  VGSSFVDADNGPDSALLHRELKWLIEDAVEDKSVSSELRTNSEQNHEHNHRNVRLKVGIE 135

Query: 121 ELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEAL 180
           ELY +WKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLV +VVSDNEAL
Sbjct: 136 ELYGVWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVAEVVSDNEAL 195

Query: 181 REGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQG 240
           REGLWVDLGTGSGAIAI ICR+LEGR RVIATDLS  AL VAGYNVQRYGLQD IELRQG
Sbjct: 196 REGLWVDLGTGSGAIAIAICRVLEGRARVIATDLSPTALTVAGYNVQRYGLQDSIELRQG 255

Query: 241 SWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCDE 300
           SWYEPL+DVQGKLSGIISNPPYIPSDNI GLQAEVGKHEPRVALDGGTNGMDELIHLCDE
Sbjct: 256 SWYEPLKDVQGKLSGIISNPPYIPSDNIVGLQAEVGKHEPRVALDGGTNGMDELIHLCDE 315

Query: 301 ATVMLKPGGFFAFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFITGFLN 358
           A VMLKPGGFFAFETNGEDQCKHLV YME+NH+G+ CNLKIVSDFA IPRFITGFLN
Sbjct: 316 AAVMLKPGGFFAFETNGEDQCKHLVKYMEDNHRGRLCNLKIVSDFAGIPRFITGFLN 372

BLAST of CSPI03G24900 vs. NCBI nr
Match: XP_023534998.1 (uncharacterized protein LOC111796557 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 624.4 bits (1609), Expect = 6.1e-175
Identity = 302/357 (84.59%), Postives = 318/357 (89.08%), Query Frame = 0

Query: 1   MRLSIARAYLVAPHRAKSIRPICSSSLNPQIPLFLRPPNYSVTLKDFHKWHNWAKILNCS 60
           MRLSI+RAYL APHR    R ICSSS  P+IPLFLRPPNYS TL D  KWH+WAK L+ S
Sbjct: 13  MRLSISRAYLAAPHRVSPSRSICSSSSKPRIPLFLRPPNYSATLTDLQKWHDWAKTLSSS 72

Query: 61  VGSSFVDTDNGPDSTLLHRELKWLVQDAVEDKSLSSELENEIEQNPELGLRNVRLKMGIE 120
           VGSSFVD DNGPDS LLHRELKWL+ DAVEDKS+SSEL    EQN E  LRNVRLK+GIE
Sbjct: 73  VGSSFVDADNGPDSALLHRELKWLIDDAVEDKSVSSELRTNSEQNHEHNLRNVRLKVGIE 132

Query: 121 ELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEAL 180
           ELY LWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLV +VVSDNEAL
Sbjct: 133 ELYGLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVAEVVSDNEAL 192

Query: 181 REGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQG 240
           REGLWVDLGTGSGAIAI ICR+LEGRGRVIATDLS  AL VAGYNVQRYGLQ+ IELRQG
Sbjct: 193 REGLWVDLGTGSGAIAIAICRVLEGRGRVIATDLSPTALTVAGYNVQRYGLQESIELRQG 252

Query: 241 SWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCDE 300
           SWYEPL+DV+G LSGIISNPPYIPS NI GLQAEVGKHEPRVALDGGTNGMDELIHLCDE
Sbjct: 253 SWYEPLKDVRGMLSGIISNPPYIPSGNIVGLQAEVGKHEPRVALDGGTNGMDELIHLCDE 312

Query: 301 ATVMLKPGGFFAFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFITGFLN 358
           A VMLKPGGFFAFETNGEDQCKHLV YME+NH+G+ C+LKIVSDFA IPRF TGFLN
Sbjct: 313 AAVMLKPGGFFAFETNGEDQCKHLVKYMEDNHRGRLCDLKIVSDFAGIPRFTTGFLN 369

BLAST of CSPI03G24900 vs. TAIR 10
Match: AT5G64150.1 (RNA methyltransferase family protein )

HSP 1 Score: 426.8 bits (1096), Expect = 1.7e-119
Identity = 203/341 (59.53%), Postives = 269/341 (78.89%), Query Frame = 0

Query: 16  AKSIRPICSSSLNPQIPLFLRPPNYSVTLKDFHKWHNWAKILNCSVGSSFVDTDNGPDST 75
           +KS RP  S S+ P+ PL+LR P+++ +L +  KWH+WAK L  SV  S  ++++  DS 
Sbjct: 38  SKSSRPTKSLSITPKTPLYLRTPSHATSLSEVWKWHDWAKDLASSVEESSKNSEDTLDSV 97

Query: 76  LLHRELKWLVQDAVEDKSLSSELENEIEQNPELGLRNVRLKMGIEELYRLWKQRIHERRP 135
           +LHRELKWL++D++ D  L     +EI  N   G +NV+L+  +EELY LW+QRI +RRP
Sbjct: 98  ILHRELKWLIEDSIVDDPLVILHRSEIADN---GEKNVKLRASLEELYDLWRQRIEKRRP 157

Query: 136 FQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDNEALREGLWVDLGTGSGAI 195
           FQY+VGCEHWRDL+L VEEGVLIPRPETE++VD+VE++V+ +E  ++ +W DLGTGSGAI
Sbjct: 158 FQYVVGCEHWRDLVLCVEEGVLIPRPETELIVDMVEELVTRDEWFKKDIWADLGTGSGAI 217

Query: 196 AIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIELRQGSWYEPLQDVQGKLSG 255
           AIGI ++L  RGRVIATDLS +A+AVAG+NVQRY L+ +IE+R+GSW+EPL+D++GKL G
Sbjct: 218 AIGIAKVLGSRGRVIATDLSPVAIAVAGHNVQRYSLEGMIEVREGSWFEPLKDLEGKLVG 277

Query: 256 IISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIHLCDEATVMLKPGGFFAFET 315
           ++SNPPYIPSD+I GLQAEVG+HEP++ALDGG +G D L HLC  A+ ML+PGGFF FET
Sbjct: 278 LVSNPPYIPSDDIPGLQAEVGRHEPKLALDGGIDGTDSLFHLCHGASRMLQPGGFFVFET 337

Query: 316 NGEDQCKHLVNY-MENNHKGKFCNLKIVSDFASIPRFITGF 356
           NGE Q K +V+Y M ++ K  F +LKIVSDFA I RF+TGF
Sbjct: 338 NGEKQSKMIVDYMMTSDLKDCFSDLKIVSDFAGINRFVTGF 375