CSPI03G18960 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G18960
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionNigrin b-like
LocationChr3: 14624274 .. 14625297 (-)
RNA-Seq ExpressionCSPI03G18960
SyntenyCSPI03G18960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTAGCCTTGTATTGTATTGTGTGTGTATATGTACCCTTCATTCTAGCCCAACTTTATCACACAAAAATCTATTATCAAATAAAGCAATCAAAAGATGAAAGAAATTGTGTTGAGCATTGTTGTAGCCTTCTCTCTCACCACCCATCTTGCCATGGCAGTGCCCTCCGACTATGGATATCAAGTCGTCCCTGGAGCCACACATCTTGTGGGTCGAGATGGATTGTGTTTGGAGATGTCTCCATGGTTTTACAAAGGTAGTAATTTTCCAACTCGATTATCACCCTGCAGCGAACAAAAGAAACAAACTCAGTTATGGACTGTTCTCCAAGATGAAACGATTCGACCAATGAATGATAGATATTGCTTGGTTTCTTACGTCTCTGTTAACTTCATAACCAATGTGGTGGTAGTAAGCGAGTGTGCTAAAGAGCCACATTCTAATAAGAAATGGATCCATAAGGAGGACAGAACCATAGTCCATGTTGGGTCAGGAATGGTTCTAACAGGAAATTCAAACTATGTGACAGTACAAAGGAACAAAAATGCACCATCACAAAGCTGGGAAGCAACGAAAAGTTTAACCCCAATGATTGCAAACATAAAATGGCTTGAAAACTTGTGTTTGCAATCGACGAAAGATTCAAATTATGTACAGTTGGATGGATGTAATAGTGAAAATAAGAGCCAACATTGGTCATTGTATGGAGATGGAACCATTAGAAAACATGTGAATAGAGACTATTGCTTGACTTCTGAACAAGATTTTGGTCGTTTTGTAGCTGTGTCTAAATGTGAAGACAAACCACAACAACGTTGGGGTCTTGGTGCTGAAGACAATACAATTAACCATCCCAATACTGACATGGTCATGGATGTGCTTAGTGTGCCTTTTGATGATCTTCCATTAGCGGTCGTTACAAACCACCGTGATGGAAGTGCTACCCAACGATGGATTATTTACTAATTAATCATATAAATAAGAAGCTAAGGGATATGTGAATAAGCACAAACTAATGCACGCAAA

mRNA sequence

CTAGCCTTGTATTGTATTGTGTGTGTATATGTACCCTTCATTCTAGCCCAACTTTATCACACAAAAATCTATTATCAAATAAAGCAATCAAAAGATGAAAGAAATTGTGTTGAGCATTGTTGTAGCCTTCTCTCTCACCACCCATCTTGCCATGGCAGTGCCCTCCGACTATGGATATCAAGTCGTCCCTGGAGCCACACATCTTGTGGGTCGAGATGGATTGTGTTTGGAGATGTCTCCATGGTTTTACAAAGGTAGTAATTTTCCAACTCGATTATCACCCTGCAGCGAACAAAAGAAACAAACTCAGTTATGGACTGTTCTCCAAGATGAAACGATTCGACCAATGAATGATAGATATTGCTTGGTTTCTTACGTCTCTGTTAACTTCATAACCAATGTGGTGGTAGTAAGCGAGTGTGCTAAAGAGCCACATTCTAATAAGAAATGGATCCATAAGGAGGACAGAACCATAGTCCATGTTGGGTCAGGAATGGTTCTAACAGGAAATTCAAACTATGTGACAGTACAAAGGAACAAAAATGCACCATCACAAAGCTGGGAAGCAACGAAAAGTTTAACCCCAATGATTGCAAACATAAAATGGCTTGAAAACTTGTGTTTGCAATCGACGAAAGATTCAAATTATGTACAGTTGGATGGATGTAATAGTGAAAATAAGAGCCAACATTGGTCATTGTATGGAGATGGAACCATTAGAAAACATGTGAATAGAGACTATTGCTTGACTTCTGAACAAGATTTTGGTCGTTTTGTAGCTGTGTCTAAATGTGAAGACAAACCACAACAACGTTGGGGTCTTGGTGCTGAAGACAATACAATTAACCATCCCAATACTGACATGGTCATGGATGTGCTTAGTGTGCCTTTTGATGATCTTCCATTAGCGGTCGTTACAAACCACCGTGATGGAAGTGCTACCCAACGATGGATTATTTACTAATTAATCATATAAATAAGAAGCTAAGGGATATGTGAATAAGCACAAACTAATGCACGCAAA

Coding sequence (CDS)

ATGAAAGAAATTGTGTTGAGCATTGTTGTAGCCTTCTCTCTCACCACCCATCTTGCCATGGCAGTGCCCTCCGACTATGGATATCAAGTCGTCCCTGGAGCCACACATCTTGTGGGTCGAGATGGATTGTGTTTGGAGATGTCTCCATGGTTTTACAAAGGTAGTAATTTTCCAACTCGATTATCACCCTGCAGCGAACAAAAGAAACAAACTCAGTTATGGACTGTTCTCCAAGATGAAACGATTCGACCAATGAATGATAGATATTGCTTGGTTTCTTACGTCTCTGTTAACTTCATAACCAATGTGGTGGTAGTAAGCGAGTGTGCTAAAGAGCCACATTCTAATAAGAAATGGATCCATAAGGAGGACAGAACCATAGTCCATGTTGGGTCAGGAATGGTTCTAACAGGAAATTCAAACTATGTGACAGTACAAAGGAACAAAAATGCACCATCACAAAGCTGGGAAGCAACGAAAAGTTTAACCCCAATGATTGCAAACATAAAATGGCTTGAAAACTTGTGTTTGCAATCGACGAAAGATTCAAATTATGTACAGTTGGATGGATGTAATAGTGAAAATAAGAGCCAACATTGGTCATTGTATGGAGATGGAACCATTAGAAAACATGTGAATAGAGACTATTGCTTGACTTCTGAACAAGATTTTGGTCGTTTTGTAGCTGTGTCTAAATGTGAAGACAAACCACAACAACGTTGGGGTCTTGGTGCTGAAGACAATACAATTAACCATCCCAATACTGACATGGTCATGGATGTGCTTAGTGTGCCTTTTGATGATCTTCCATTAGCGGTCGTTACAAACCACCGTGATGGAAGTGCTACCCAACGATGGATTATTTACTAA

Protein sequence

MKEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPWFYKGSNFPTRLSPCSEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFITNVVVVSECAKEPHSNKKWIHKEDRTIVHVGSGMVLTGNSNYVTVQRNKNAPSQSWEATKSLTPMIANIKWLENLCLQSTKDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRDYCLTSEQDFGRFVAVSKCEDKPQQRWGLGAEDNTINHPNTDMVMDVLSVPFDDLPLAVVTNHRDGSATQRWIIY*
Homology
BLAST of CSPI03G18960 vs. ExPASy Swiss-Prot
Match: Q41358 (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 3.8e-29
Identity = 84/264 (31.82%), Postives = 131/264 (49.62%), Query Frame = 0

Query: 30  VVPGATHLVGRDGLCLEMSPWFYKGSNFPTRLSPCSEQKKQTQLWTVLQDETIRPMNDRY 89
           VV     + G DGLC+++    Y   N P +L PC  +    QLWT   D TIR +    
Sbjct: 318 VVEVTRRISGWDGLCVDVRYGHYIDGN-PVQLRPCGNE--CNQLWTFRTDGTIRWLGK-- 377

Query: 90  CLVSYVSVNFITNVVVVSECAKEPHSNKKWIHKEDRTIVHVGSGMVLT----GNSNYVTV 149
           CL +       ++ V++ +C   P    KW+   D TI +  SG+VLT         +++
Sbjct: 378 CLTA-------SSSVMIYDCNTVPPEATKWVVSIDGTITNPHSGLVLTAPQAAEGTALSL 437

Query: 150 QRNKNAPSQSWEATKSLTPMIANIKWLENLCLQSTKDSNYVQLDGCNSENKSQHWSLYGD 209
           + N +A  Q W     + P++  I   + +CL+   ++N+V L+ C      Q W+LYGD
Sbjct: 438 ENNIHAARQGW-TVGDVEPLVTFIVGYKQMCLRENGENNFVWLEDCVLNRVQQEWALYGD 497

Query: 210 GTIRKHVNRDYCLTSE-QDFGRFVAVSKCEDKPQQRWGLGAEDNTINHPNTDMVMDVLSV 269
           GTIR + NR  C+TSE  +    + + KCE    QRW     + TI++PN  ++MDV   
Sbjct: 498 GTIRVNSNRSLCVTSEDHEPSDLIVILKCEGSGNQRWVFNT-NGTISNPNAKLLMDVAQ- 557

Query: 270 PFDDLPLAVVTNHR-DGSATQRWI 288
              D+ L  +  +R  G+  Q+WI
Sbjct: 558 --RDVSLRKIILYRPTGNPNQQWI 564

BLAST of CSPI03G18960 vs. ExPASy Swiss-Prot
Match: P93543 (Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.5e-28
Identity = 84/258 (32.56%), Postives = 133/258 (51.55%), Query Frame = 0

Query: 39  GRDGLCLEMSPWFYKGSNFPTRLSPCSEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVN 98
           GRDG C E+     K    P +LS C EQ  Q   WT   D TI+ +    CL +     
Sbjct: 323 GRDGFCAEVKNGDEKDGT-PVQLSSCGEQSNQQ--WTFSTDGTIQSLGK--CLTT----- 382

Query: 99  FITNVVVVSECAKEPHSNKKWIHKEDRTIVHVGSGMVLT----GNSNYVTVQRNKNAPSQ 158
             ++ V++  C   P  + KW+   D TI +  SG+VLT         V++++N +A  Q
Sbjct: 383 --SSSVMIYNCKVVPPESTKWVVSIDGTITNPRSGLVLTAPKAAEGTLVSLEKNVHAARQ 442

Query: 159 SWEATKSLTPMIANIKWLENLCLQSTKDSNYVQLDGCNSENKS---QHWSLYGDGTIRKH 218
            W    ++ P++  I   E +CL++   +N V L  C+ ++ S   Q W+LYGDGTIR +
Sbjct: 443 GW-IVGNVEPLVTFIVGYEQMCLETNPGNNDVSLGDCSVKSASKVDQKWALYGDGTIRVN 502

Query: 219 VNRDYCLTSE-QDFGRFVAVSKCEDKPQQRWGLGAEDNTINHPNTDMVMDVLSVPFDDLP 278
            +R  C+TSE +     + + KC     QRW     D TI++P++ +VM    V  +D+P
Sbjct: 503 NDRSLCVTSEGKSSNEPIIILKCLGWANQRWVFNT-DGTISNPDSKLVM---HVDQNDVP 562

Query: 279 L-AVVTNHRDGSATQRWI 288
           L  ++ +H  G++ Q+WI
Sbjct: 563 LRKIILSHPSGTSNQQWI 563

BLAST of CSPI03G18960 vs. ExPASy Swiss-Prot
Match: P33183 (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 126.7 bits (317), Expect = 4.2e-28
Identity = 80/259 (30.89%), Postives = 137/259 (52.90%), Query Frame = 0

Query: 36  HLVGRDGLCLEMSPWFYKGSNFPTRLSPCSEQKKQTQLWTVLQDETIRPMNDRYCLVSYV 95
           ++VGRDGLC+++    Y     P +L PC  Q+ Q   WT   D+TIR M    C+ +  
Sbjct: 311 NIVGRDGLCVDVRNG-YDTDGTPLQLWPCGTQRNQR--WTFDSDDTIRSMGK--CMTAN- 370

Query: 96  SVNFITNVVVVSECAKEPHSNKKWIHKEDRTIVHVGSGMVLT----GNSNYVTVQRNKNA 155
            +N  +N+V+ + C+    +  KW    D +I++  SG+V+T     +   + ++ N  A
Sbjct: 371 GLNNGSNIVIFN-CSTAAENAIKWEVPIDGSIINPSSGLVMTAPRAASRTILLLEDNIYA 430

Query: 156 PSQSWEATKSLTPMIANIKWLENLCLQSTKDSNYVQLDGCNSENKSQHWSLYGDGTIRKH 215
            SQ W  T ++ P++A+I   + +CLQS  ++N V ++ C + +  Q W+LYGD TIR +
Sbjct: 431 ASQGWTVTNNVKPIVASIVGYKEMCLQSNGENNGVWMEDCEATSLQQQWALYGDRTIRVN 490

Query: 216 VNRDYCLTSE-QDFGRFVAVSKCEDKPQQRWGLGAEDNTINHPNTDMVMDV--LSVPFDD 275
             R  C+T+   +    + + KC+  P QRW   + D  I +P +  VMDV   +V   +
Sbjct: 491 STRGLCVTTNGYNSKDLIIILKCQGLPSQRWFFNS-DGAIVNPKSRHVMDVRASNVSLRE 550

Query: 276 LPLAVVTNHRDGSATQRWI 288
           + +   T    G+  Q+W+
Sbjct: 551 IIIFPAT----GNPNQQWV 557

BLAST of CSPI03G18960 vs. ExPASy Swiss-Prot
Match: P06750 (Agglutinin OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 5.5e-28
Identity = 88/258 (34.11%), Postives = 134/258 (51.94%), Query Frame = 0

Query: 37  LVGRDGLCLEMS-PWFYKGSNFPTRLSPCSEQKKQTQLWTVLQDETIRPMNDRYCLVSYV 96
           +VGR+GLC++++   F+ G+  P +L PC       QLWT+ +D TIR  ++  CL   +
Sbjct: 315 IVGRNGLCVDVTGEEFFDGN--PIQLWPCKSNTDWNQLWTLRKDSTIR--SNGKCLT--I 374

Query: 97  SVNFITNVVVVSECAKEPHSNKKWIHKEDRTIVHVGSGMVL---TGNS-NYVTVQRNKNA 156
           S +     VV+  C+       +W   ++RTI++  SG+VL   +GNS   +TVQ N  A
Sbjct: 375 SKSSPRQQVVIYNCSTATVGATRWQIWDNRTIINPRSGLVLAATSGNSGTKLTVQTNIYA 434

Query: 157 PSQSWEATKSLTPMIANIKWLENLCLQSTKDSNYVQLDGCNSENKSQHWSLYGDGTIRKH 216
            SQ W  T +  P +  I  L  +CLQ+  +S  V L+ C SE   Q W+LY DG+IR  
Sbjct: 435 VSQGWLPTNNTQPFVTTIVGLYGMCLQA--NSGKVWLEDCTSEKAEQQWALYADGSIRPQ 494

Query: 217 VNRDYCLTSEQDF-GRFVAVSKCEDKPQ-QRWGLGAEDNTINHPNTDMVMDVLSVPFDDL 276
            NRD CLT++ +  G  V +  C      QRW +   D TI +    +V+DV     D  
Sbjct: 495 QNRDNCLTTDANIKGTVVKILSCGPASSGQRW-MFKNDGTILNLYNGLVLDVRR--SDPS 554

Query: 277 PLAVVTNHRDGSATQRWI 288
              ++ +   G+  Q W+
Sbjct: 555 LKQIIVHPFHGNLNQIWL 561

BLAST of CSPI03G18960 vs. ExPASy Swiss-Prot
Match: O22415 (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 5.5e-28
Identity = 82/264 (31.06%), Postives = 128/264 (48.48%), Query Frame = 0

Query: 30  VVPGATHLVGRDGLCLEMSPWFYKGSNFPTRLSPCSEQKKQTQLWTVLQDETIRPMNDRY 89
           VV     + G DGLC+++    Y   N   +L PC  +    QLWT   D TIR +    
Sbjct: 318 VVEVTRRISGWDGLCVDVRDGHYIDGN-TVQLGPCGNE--CNQLWTFRTDGTIRWLGK-- 377

Query: 90  CLVSYVSVNFITNVVVVSECAKEPHSNKKWIHKEDRTIVHVGSGMVLT----GNSNYVTV 149
           CL +       ++ V++ +C   P    KW+   D TI +  SG+VLT         +++
Sbjct: 378 CLTT-------SSSVMIYDCNTVPPEATKWVVSTDGTITNPRSGLVLTAPQAAEGTALSL 437

Query: 150 QRNKNAPSQSWEATKSLTPMIANIKWLENLCLQSTKDSNYVQLDGCNSENKSQHWSLYGD 209
           + N +A  Q W     + P++  I   + +CL    ++N+V L+ C      Q W+LYGD
Sbjct: 438 ENNIHAARQGW-TVGDVEPLVTFIVGYKQMCLTENGENNFVWLEDCVLNRVEQEWALYGD 497

Query: 210 GTIRKHVNRDYCLTSE-QDFGRFVAVSKCEDKPQQRWGLGAEDNTINHPNTDMVMDVLSV 269
           GTIR + NR  C+TSE  +    + + KCE    QRW     + TI++PN  +VMDV   
Sbjct: 498 GTIRVNSNRSLCVTSEDHEPSDLIVILKCEGSGNQRWVFNT-NGTISNPNAKLVMDVAQ- 557

Query: 270 PFDDLPL-AVVTNHRDGSATQRWI 288
              ++ L  ++     G+  Q+WI
Sbjct: 558 --SNVSLRKIILYPPTGNPNQQWI 564

BLAST of CSPI03G18960 vs. ExPASy TrEMBL
Match: A0A0A0L756 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G219180 PE=4 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 5.3e-167
Identity = 284/289 (98.27%), Postives = 288/289 (99.65%), Query Frame = 0

Query: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPWFYKGSNFPTR 60
           MKEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPWFYKGSNFPTR
Sbjct: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPWFYKGSNFPTR 60

Query: 61  LSPCSEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFITNVVVVSECAKEPHSNKKWI 120
           LSPC+EQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFITN VVVSECAKEPHSNKKWI
Sbjct: 61  LSPCNEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFITN-VVVSECAKEPHSNKKWI 120

Query: 121 HKEDRTIVHVGSGMVLTGNSNYVTVQRNKNAPSQSWEATKSLTPMIANIKWLENLCLQST 180
           HKEDRTIVHVGSGMVLTGNSNYVTVQRNKNAPSQSWEATKSLTPM+ANIKWLENLCLQST
Sbjct: 121 HKEDRTIVHVGSGMVLTGNSNYVTVQRNKNAPSQSWEATKSLTPMVANIKWLENLCLQST 180

Query: 181 KDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRDYCLTSEQDFGRFVAVSKCEDKPQQR 240
           KDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNR+YCLTSEQDFGRFVAVSKCEDKPQQR
Sbjct: 181 KDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRNYCLTSEQDFGRFVAVSKCEDKPQQR 240

Query: 241 WGLGAEDNTINHPNTDMVMDVLSVPFDDLPLAVVTNHRDGSATQRWIIY 290
           WGLGAEDNTINHPNTDMVMDVLSVPFDDLPLAVVTNHRDG+ATQRWIIY
Sbjct: 241 WGLGAEDNTINHPNTDMVMDVLSVPFDDLPLAVVTNHRDGTATQRWIIY 288

BLAST of CSPI03G18960 vs. ExPASy TrEMBL
Match: A0A5A7T854 (Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G001540 PE=3 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.5e-124
Identity = 218/292 (74.66%), Postives = 249/292 (85.27%), Query Frame = 0

Query: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYG----YQVVPGATHLVGRDGLCLEMSPWFYKGSN 60
           MKEIVLSI+VAFSLTTHLAMAVPS+YG    Y  VPGATHLVGRDGLCLEMSPWFY+G+N
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPSNYGYGGEYGGVPGATHLVGRDGLCLEMSPWFYRGNN 60

Query: 61  FPTRLSPCSEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFITNVVVVSECAKEPHSN 120
           FPTRLSPC E KKQTQLWTV+ D TIRPMND++CLV+ V V  I N  VV ECAKE +SN
Sbjct: 61  FPTRLSPCDEHKKQTQLWTVVGDGTIRPMNDKFCLVADVLVGVI-NKAVVRECAKESNSN 120

Query: 121 KKWIHKEDRTIVHVGSGMVLTGNSNYVTVQRNKNAPSQSWEATKSLTPMIANIKWLENLC 180
           K+W HK+D TI HV SGMVLTG+S+YVT+Q NKN PSQSWEAT+S TPMIANI+WL NLC
Sbjct: 121 KRWTHKKDGTITHVESGMVLTGDSDYVTLQSNKNTPSQSWEATESSTPMIANIEWLNNLC 180

Query: 181 LQSTKDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRDYCLTSEQDFGRFVAVSKCEDK 240
           LQST DSN+V L+GCN ENK Q W+LYGDGTIR++VNR+YCLTS++  GRF+ +SKCEDK
Sbjct: 181 LQSTDDSNHVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLTSDEYLGRFIVMSKCEDK 240

Query: 241 PQQRWGLGAEDNTINHPNTDMVMDVLSVPFDDLPLAVVTNHRDGSATQRWII 289
           PQQRW LGA+D+TI+HPNTDMVMDVL VP+ D   AVVTN RDGSA+QRW I
Sbjct: 241 PQQRWNLGAKDSTIDHPNTDMVMDVLFVPYYDFSSAVVTNRRDGSASQRWTI 291

BLAST of CSPI03G18960 vs. ExPASy TrEMBL
Match: A0A1S4E0X9 (nigrin b-like OS=Cucumis melo OX=3656 GN=LOC103496121 PE=4 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.3e-124
Identity = 217/292 (74.32%), Postives = 249/292 (85.27%), Query Frame = 0

Query: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYG----YQVVPGATHLVGRDGLCLEMSPWFYKGSN 60
           MKEIVLSI+VAFSLTTHLAMAVPS+YG    Y  VP ATHLVGRDGLCLEMSPWFY+G+N
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPSNYGYGGEYGGVPRATHLVGRDGLCLEMSPWFYRGNN 60

Query: 61  FPTRLSPCSEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFITNVVVVSECAKEPHSN 120
           FPTRLSPC E KKQTQLWTV+ D TIRPMND++CLV+ V V  I N  VV ECAKEP+SN
Sbjct: 61  FPTRLSPCDEHKKQTQLWTVVGDGTIRPMNDKFCLVADVLVGLI-NKAVVRECAKEPNSN 120

Query: 121 KKWIHKEDRTIVHVGSGMVLTGNSNYVTVQRNKNAPSQSWEATKSLTPMIANIKWLENLC 180
           K+W HK+D TI HV SGMVLTG+S+YVT+Q NKN PSQSWEAT+S TPMIANI+WL NLC
Sbjct: 121 KRWTHKKDGTITHVESGMVLTGDSDYVTLQSNKNTPSQSWEATESSTPMIANIEWLNNLC 180

Query: 181 LQSTKDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRDYCLTSEQDFGRFVAVSKCEDK 240
           LQST DSN+V L+GCN ENK Q W+LYGDGTIR++VNR+YCLTS++  GRF+ +SKCEDK
Sbjct: 181 LQSTDDSNHVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLTSDEYLGRFIVMSKCEDK 240

Query: 241 PQQRWGLGAEDNTINHPNTDMVMDVLSVPFDDLPLAVVTNHRDGSATQRWII 289
           PQQRW LGA+D+TI+HPN+DMVMDVL VP+ D   AVVTN RDGSA+QRW I
Sbjct: 241 PQQRWNLGAKDSTIDHPNSDMVMDVLFVPYYDFSSAVVTNRRDGSASQRWTI 291

BLAST of CSPI03G18960 vs. ExPASy TrEMBL
Match: A0A5D3BL95 (Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold252G00260 PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 7.3e-124
Identity = 217/292 (74.32%), Postives = 248/292 (84.93%), Query Frame = 0

Query: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYG----YQVVPGATHLVGRDGLCLEMSPWFYKGSN 60
           MKEIVLSI+VAFSLTTHLAMAVP +YG    Y  VPGATHLVGRDGLCLEMSPWFY+G+N
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPYNYGYGGEYGGVPGATHLVGRDGLCLEMSPWFYRGNN 60

Query: 61  FPTRLSPCSEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFITNVVVVSECAKEPHSN 120
           FPTRLSPC E KKQTQLWTV+ D TIRPMND++CLV+ V V  I N  VV ECAKE +SN
Sbjct: 61  FPTRLSPCDEHKKQTQLWTVVGDGTIRPMNDKFCLVADVLVGVI-NKAVVRECAKESNSN 120

Query: 121 KKWIHKEDRTIVHVGSGMVLTGNSNYVTVQRNKNAPSQSWEATKSLTPMIANIKWLENLC 180
           K+W HK+D TI HV SGMVLTG+S+YVT+Q NKN PSQSWEAT+S TPMIANI+WL NLC
Sbjct: 121 KRWTHKKDGTITHVESGMVLTGDSDYVTLQSNKNTPSQSWEATESSTPMIANIEWLNNLC 180

Query: 181 LQSTKDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRDYCLTSEQDFGRFVAVSKCEDK 240
           LQST DSN+V L+GCN ENK Q W+LYGDGTIR++VNR+YCLTS++  GRF+ +SKCEDK
Sbjct: 181 LQSTDDSNHVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLTSDEYLGRFIVMSKCEDK 240

Query: 241 PQQRWGLGAEDNTINHPNTDMVMDVLSVPFDDLPLAVVTNHRDGSATQRWII 289
           PQQRW LGA+D+TI+HPNTDMVMDVL VP+ D   AVVTN RDGSA+QRW I
Sbjct: 241 PQQRWNLGAKDSTIDHPNTDMVMDVLFVPYYDFSSAVVTNRRDGSASQRWTI 291

BLAST of CSPI03G18960 vs. ExPASy TrEMBL
Match: Q9SLV9 (XSP30 OS=Cucumis sativus OX=3659 GN=XSP30 PE=2 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 3.0e-109
Identity = 200/293 (68.26%), Postives = 230/293 (78.50%), Query Frame = 0

Query: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYGYQV----VPGATHLVGRDGLCLEMSPWFY-KGS 60
           MKEIVLSI+VAFSLTT LA+AVP +YGY V    VPGATHLVGRDGLCLEMSPW+   G 
Sbjct: 1   MKEIVLSIIVAFSLTTQLAIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMSPWYKPAGI 60

Query: 61  NFPTRLSPCSEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFITNVVVVSECAKEPHS 120
           NFPTRLSPC E KKQTQLWT++ D TIRPMND++CL + V    I N  VVSEC K    
Sbjct: 61  NFPTRLSPCDE-KKQTQLWTIVGDGTIRPMNDKFCLAAEVFYGVI-NKAVVSECGKVSDP 120

Query: 121 NKKWIHKEDRTIVHVGSGMVLTGNSNYVTVQRNKNAPSQSWEATKSLTPMIANIKWLENL 180
           NKKW  K D TI  V S MVLTG+ +YVT+Q NK  PSQSWE T+SL  M+ANI+WL NL
Sbjct: 121 NKKWTQKNDGTIALVDSRMVLTGDLDYVTLQSNKYTPSQSWEVTESLNSMVANIEWLNNL 180

Query: 181 CLQSTKDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRDYCLTSEQDFGRFVAVSKCED 240
           CLQST DS++V L+GCN++NK Q W+LY DGTIR+HVN++YCLTS+QDFGRFV VSKCED
Sbjct: 181 CLQSTDDSSHVGLNGCNTDNKYQRWALYADGTIRQHVNKNYCLTSDQDFGRFVVVSKCED 240

Query: 241 KPQQRWGLGAEDNTINHPNTDMVMDVLSVPFDDLPLAVVTNHRDGSATQRWII 289
           KPQQRW L A+D TI+HPNTDMV+DV SVP    P  +VTN RDGSA+QRW I
Sbjct: 241 KPQQRWSLDAKDYTIDHPNTDMVLDVFSVPDSTFPSVLVTNRRDGSASQRWTI 291

BLAST of CSPI03G18960 vs. NCBI nr
Match: XP_004146289.1 (nigrin b [Cucumis sativus] >KGN57583.1 hypothetical protein Csa_010930 [Cucumis sativus])

HSP 1 Score: 596.7 bits (1537), Expect = 1.1e-166
Identity = 284/289 (98.27%), Postives = 288/289 (99.65%), Query Frame = 0

Query: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPWFYKGSNFPTR 60
           MKEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPWFYKGSNFPTR
Sbjct: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPWFYKGSNFPTR 60

Query: 61  LSPCSEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFITNVVVVSECAKEPHSNKKWI 120
           LSPC+EQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFITN VVVSECAKEPHSNKKWI
Sbjct: 61  LSPCNEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFITN-VVVSECAKEPHSNKKWI 120

Query: 121 HKEDRTIVHVGSGMVLTGNSNYVTVQRNKNAPSQSWEATKSLTPMIANIKWLENLCLQST 180
           HKEDRTIVHVGSGMVLTGNSNYVTVQRNKNAPSQSWEATKSLTPM+ANIKWLENLCLQST
Sbjct: 121 HKEDRTIVHVGSGMVLTGNSNYVTVQRNKNAPSQSWEATKSLTPMVANIKWLENLCLQST 180

Query: 181 KDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRDYCLTSEQDFGRFVAVSKCEDKPQQR 240
           KDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNR+YCLTSEQDFGRFVAVSKCEDKPQQR
Sbjct: 181 KDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRNYCLTSEQDFGRFVAVSKCEDKPQQR 240

Query: 241 WGLGAEDNTINHPNTDMVMDVLSVPFDDLPLAVVTNHRDGSATQRWIIY 290
           WGLGAEDNTINHPNTDMVMDVLSVPFDDLPLAVVTNHRDG+ATQRWIIY
Sbjct: 241 WGLGAEDNTINHPNTDMVMDVLSVPFDDLPLAVVTNHRDGTATQRWIIY 288

BLAST of CSPI03G18960 vs. NCBI nr
Match: KAA0039058.1 (nigrin b-like [Cucumis melo var. makuwa])

HSP 1 Score: 455.7 bits (1171), Expect = 3.0e-124
Identity = 218/292 (74.66%), Postives = 249/292 (85.27%), Query Frame = 0

Query: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYG----YQVVPGATHLVGRDGLCLEMSPWFYKGSN 60
           MKEIVLSI+VAFSLTTHLAMAVPS+YG    Y  VPGATHLVGRDGLCLEMSPWFY+G+N
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPSNYGYGGEYGGVPGATHLVGRDGLCLEMSPWFYRGNN 60

Query: 61  FPTRLSPCSEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFITNVVVVSECAKEPHSN 120
           FPTRLSPC E KKQTQLWTV+ D TIRPMND++CLV+ V V  I N  VV ECAKE +SN
Sbjct: 61  FPTRLSPCDEHKKQTQLWTVVGDGTIRPMNDKFCLVADVLVGVI-NKAVVRECAKESNSN 120

Query: 121 KKWIHKEDRTIVHVGSGMVLTGNSNYVTVQRNKNAPSQSWEATKSLTPMIANIKWLENLC 180
           K+W HK+D TI HV SGMVLTG+S+YVT+Q NKN PSQSWEAT+S TPMIANI+WL NLC
Sbjct: 121 KRWTHKKDGTITHVESGMVLTGDSDYVTLQSNKNTPSQSWEATESSTPMIANIEWLNNLC 180

Query: 181 LQSTKDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRDYCLTSEQDFGRFVAVSKCEDK 240
           LQST DSN+V L+GCN ENK Q W+LYGDGTIR++VNR+YCLTS++  GRF+ +SKCEDK
Sbjct: 181 LQSTDDSNHVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLTSDEYLGRFIVMSKCEDK 240

Query: 241 PQQRWGLGAEDNTINHPNTDMVMDVLSVPFDDLPLAVVTNHRDGSATQRWII 289
           PQQRW LGA+D+TI+HPNTDMVMDVL VP+ D   AVVTN RDGSA+QRW I
Sbjct: 241 PQQRWNLGAKDSTIDHPNTDMVMDVLFVPYYDFSSAVVTNRRDGSASQRWTI 291

BLAST of CSPI03G18960 vs. NCBI nr
Match: XP_016901886.1 (PREDICTED: nigrin b-like [Cucumis melo])

HSP 1 Score: 454.5 bits (1168), Expect = 6.8e-124
Identity = 217/292 (74.32%), Postives = 249/292 (85.27%), Query Frame = 0

Query: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYG----YQVVPGATHLVGRDGLCLEMSPWFYKGSN 60
           MKEIVLSI+VAFSLTTHLAMAVPS+YG    Y  VP ATHLVGRDGLCLEMSPWFY+G+N
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPSNYGYGGEYGGVPRATHLVGRDGLCLEMSPWFYRGNN 60

Query: 61  FPTRLSPCSEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFITNVVVVSECAKEPHSN 120
           FPTRLSPC E KKQTQLWTV+ D TIRPMND++CLV+ V V  I N  VV ECAKEP+SN
Sbjct: 61  FPTRLSPCDEHKKQTQLWTVVGDGTIRPMNDKFCLVADVLVGLI-NKAVVRECAKEPNSN 120

Query: 121 KKWIHKEDRTIVHVGSGMVLTGNSNYVTVQRNKNAPSQSWEATKSLTPMIANIKWLENLC 180
           K+W HK+D TI HV SGMVLTG+S+YVT+Q NKN PSQSWEAT+S TPMIANI+WL NLC
Sbjct: 121 KRWTHKKDGTITHVESGMVLTGDSDYVTLQSNKNTPSQSWEATESSTPMIANIEWLNNLC 180

Query: 181 LQSTKDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRDYCLTSEQDFGRFVAVSKCEDK 240
           LQST DSN+V L+GCN ENK Q W+LYGDGTIR++VNR+YCLTS++  GRF+ +SKCEDK
Sbjct: 181 LQSTDDSNHVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLTSDEYLGRFIVMSKCEDK 240

Query: 241 PQQRWGLGAEDNTINHPNTDMVMDVLSVPFDDLPLAVVTNHRDGSATQRWII 289
           PQQRW LGA+D+TI+HPN+DMVMDVL VP+ D   AVVTN RDGSA+QRW I
Sbjct: 241 PQQRWNLGAKDSTIDHPNSDMVMDVLFVPYYDFSSAVVTNRRDGSASQRWTI 291

BLAST of CSPI03G18960 vs. NCBI nr
Match: TYJ99749.1 (nigrin b-like [Cucumis melo var. makuwa])

HSP 1 Score: 453.4 bits (1165), Expect = 1.5e-123
Identity = 217/292 (74.32%), Postives = 248/292 (84.93%), Query Frame = 0

Query: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYG----YQVVPGATHLVGRDGLCLEMSPWFYKGSN 60
           MKEIVLSI+VAFSLTTHLAMAVP +YG    Y  VPGATHLVGRDGLCLEMSPWFY+G+N
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPYNYGYGGEYGGVPGATHLVGRDGLCLEMSPWFYRGNN 60

Query: 61  FPTRLSPCSEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFITNVVVVSECAKEPHSN 120
           FPTRLSPC E KKQTQLWTV+ D TIRPMND++CLV+ V V  I N  VV ECAKE +SN
Sbjct: 61  FPTRLSPCDEHKKQTQLWTVVGDGTIRPMNDKFCLVADVLVGVI-NKAVVRECAKESNSN 120

Query: 121 KKWIHKEDRTIVHVGSGMVLTGNSNYVTVQRNKNAPSQSWEATKSLTPMIANIKWLENLC 180
           K+W HK+D TI HV SGMVLTG+S+YVT+Q NKN PSQSWEAT+S TPMIANI+WL NLC
Sbjct: 121 KRWTHKKDGTITHVESGMVLTGDSDYVTLQSNKNTPSQSWEATESSTPMIANIEWLNNLC 180

Query: 181 LQSTKDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRDYCLTSEQDFGRFVAVSKCEDK 240
           LQST DSN+V L+GCN ENK Q W+LYGDGTIR++VNR+YCLTS++  GRF+ +SKCEDK
Sbjct: 181 LQSTDDSNHVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLTSDEYLGRFIVMSKCEDK 240

Query: 241 PQQRWGLGAEDNTINHPNTDMVMDVLSVPFDDLPLAVVTNHRDGSATQRWII 289
           PQQRW LGA+D+TI+HPNTDMVMDVL VP+ D   AVVTN RDGSA+QRW I
Sbjct: 241 PQQRWNLGAKDSTIDHPNTDMVMDVLFVPYYDFSSAVVTNRRDGSASQRWTI 291

BLAST of CSPI03G18960 vs. NCBI nr
Match: XP_038904853.1 (nigrin b-like [Benincasa hispida])

HSP 1 Score: 439.1 bits (1128), Expect = 2.9e-119
Identity = 214/291 (73.54%), Postives = 243/291 (83.51%), Query Frame = 0

Query: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPWFYKGSNFPTR 60
           MKEIVLSIVVAFSLTTHL MAV  +YGY   PG+THLVGRDGLCLEM PW+ K S FPTR
Sbjct: 1   MKEIVLSIVVAFSLTTHLVMAVRPNYGYGGFPGSTHLVGRDGLCLEMFPWYEKDSYFPTR 60

Query: 61  LSPCSEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFITNVVVVSECAKEPHSNKKWI 120
           LSPC+ QKKQTQLWTVLQD TIRPMND+YCLV+Y  V  + N VVV++C+KE + NK W 
Sbjct: 61  LSPCNVQKKQTQLWTVLQDGTIRPMNDKYCLVAYYRVGVLNN-VVVNDCSKESNPNKIWN 120

Query: 121 HKEDRTIVHVGSGMVLTGNSN-YVTVQRNKNAPSQSWEATKSLTPMIANIKWLENLCLQS 180
           HK+D T+VHV SGMVLTG S+ YVT++ N+N PSQSWEAT+SLTPM+ANIKWL+NLCLQS
Sbjct: 121 HKKDGTVVHVESGMVLTGKSDAYVTLEPNENVPSQSWEATESLTPMVANIKWLDNLCLQS 180

Query: 181 TKDSNYVQLDGCNSENKSQHWSLYGDGTIRKHVNRDYCLTSEQDFGRFVAVSKCEDKPQQ 240
           T+DSN+V LDGCN EN  Q W+LYGDGTIR  VNR+YCLTSEQD GRF+ VS+CEDKPQQ
Sbjct: 181 TEDSNHVGLDGCNRENMKQRWALYGDGTIRHSVNRNYCLTSEQDVGRFIVVSRCEDKPQQ 240

Query: 241 RWGLGAEDNTINHP-NTDMVMDVLSVPFDDLPLAVVTNHRDGSATQRWIIY 290
           RWGL AED+TINHP NTDMVMDV  VP   +P AVV  HRDGS +QRWIIY
Sbjct: 241 RWGLSAEDSTINHPANTDMVMDVFMVPTTLVP-AVVMGHRDGSPSQRWIIY 289

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q413583.8e-2931.82Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1[more]
P935431.5e-2832.56Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1[more]
P331834.2e-2830.89Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2[more]
P067505.5e-2834.11Agglutinin OS=Ricinus communis OX=3988 PE=1 SV=1[more]
O224155.5e-2831.06Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0L7565.3e-16798.27Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G219180 PE=4 SV=1[more]
A0A5A7T8541.5e-12474.66Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G001540 ... [more]
A0A1S4E0X93.3e-12474.32nigrin b-like OS=Cucumis melo OX=3656 GN=LOC103496121 PE=4 SV=1[more]
A0A5D3BL957.3e-12474.32Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold252G00260 ... [more]
Q9SLV93.0e-10968.26XSP30 OS=Cucumis sativus OX=3659 GN=XSP30 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004146289.11.1e-16698.27nigrin b [Cucumis sativus] >KGN57583.1 hypothetical protein Csa_010930 [Cucumis ... [more]
KAA0039058.13.0e-12474.66nigrin b-like [Cucumis melo var. makuwa][more]
XP_016901886.16.8e-12474.32PREDICTED: nigrin b-like [Cucumis melo][more]
TYJ99749.11.5e-12374.32nigrin b-like [Cucumis melo var. makuwa][more]
XP_038904853.12.9e-11973.54nigrin b-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000772Ricin B, lectin domainSMARTSM00458ricin_3coord: 163..289
e-value: 3.3E-14
score: 63.2
coord: 32..159
e-value: 4.7E-6
score: 36.1
IPR000772Ricin B, lectin domainPFAMPF00652Ricin_B_lectincoord: 173..286
e-value: 2.4E-19
score: 69.8
coord: 36..156
e-value: 1.2E-16
score: 61.1
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 162..289
score: 26.533501
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 31..159
score: 19.852501
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 174..288
e-value: 9.39061E-17
score: 72.9234
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 36..157
e-value: 2.50457E-14
score: 66.375
NoneNo IPR availableGENE3D2.80.10.50coord: 164..289
e-value: 2.8E-29
score: 103.3
NoneNo IPR availableGENE3D2.80.10.50coord: 32..159
e-value: 4.0E-22
score: 80.4
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 30..157
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 169..288

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G18960.1CSPI03G18960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0017148 negative regulation of translation
molecular_function GO:0030598 rRNA N-glycosylase activity