CSPI03G01080 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G01080
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionprotein PAM68, chloroplastic
LocationChr3: 769018 .. 770811 (-)
RNA-Seq ExpressionCSPI03G01080
SyntenyCSPI03G01080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTTTGATGAAAACCTTATCCTCTTTCACTGCTCTCATCCATGGCGTCCGTCTCTGCTTCGCATAGTGAATACAAGATGTAAGAGACGAGTCGACTGTATTTCAGTTTCATAGACAAACCTTAATGGCCGCCATTGCCGGATCTCTCAACCTTCCTCTTGCGCCTTCAAACCTTCCATTACTCCGCATTAAGGTACTCTTCTCATTTCGCTTCCCCATTGCAACATTTCTTTGAGTTCTGTAACAACCTTTTCTACTATAACTACTTCAAATTATACCTAAACGATGAAGTATTCCTGAATCCGAACTGTTTATCAATGGATTATCCATAAGTTCATCAATCAACAATCGGTTTCTTAAAGATTAGGAGTTAGGATTTAGGATTTAGGATTTACTTTATCAGGGATACTTGTTAAACCTAGAACGATCCATTGATCCATTTCAATGACTACTACTTGAATTATAATGAAACGGAAGAAGACGAAGGATTCTTTAATCTGAACTATTCATCAATGGAATTTCAGTTTTGATTAGTAGTTAGGATTTAAATTTATTCTCTAGTTTGAGACATGTTTGCGTAGGTATTAACTTCGAATGTGTTGGGTGCTCTTTGTGAAACCTACCTCGGGCATCAAGATTTCTTTTTCCATAATTTTTAGGAAAGAGTGATAATTCCTCCAAGAAGATAAAAGAATTTACTACACCTAAACGCAAAAGAACATTGGTTTGACTGTTTGAGTGTGCGAAAACATGCGTGAATGAATGTTGCTTTTAATTATTTTTATGTAGGCTTTGGTTTTCTTCAATTTTAAAGTTTTTCACGTGTGTATCCTAGTTTCTTAAATTTTTTATGTGGCTGTAGTGAGGTTTTTTACCAAGTAGGATTGATATTTATTAAACTTAGAACGACTCGTTGATTATTTTTATTTTCTCAGCAGAAAAATTGTAAATTCTCACTTCATATAAAAAGCAACGAAAGAAACTGTTTCCCAAATTTAAGACCAACCATTAACCAAAACCAAAACCAAACTCACACAAACCGCCCCACAAGTCTAAATGCAACATTGAGGCGTCCGAAGGGATTCGGACCAGCTTCAAGGAAGAAGAAGACGAAGAAAACAAGAAGAGAAGGAAGTGAGGATGATGACAACGAGGAGGAGGAAGAGGAAGACGAGGAGGAGGAAGAGGAAGGTGGTGTAATACCAGAAGTAGTGACAAATAGGATGATGAGTAGAATGGGATTTACAGTGGGGATTCCATTGTTTATAGGGCTATTGTTCTTCCCATTCTTCTATTACCTCAAAGTTGGGCTGAAGATTGATGTGCCCTCATGGGTGCCAGTCATAGTCTCATTCTTCTTCTTTGGTTCAGCTCTTTTGGGAGTTAGCTATGGGATTGTGTCCTCTAGTTGGGATCCTTTGAGGGACGGCTCTTTATTGGGTTGGAATGAAGCTCAGAAGAATTGGCCTGTTTTTTGGAAGTCCATTTGGGGTGGATCTAATAAGAAATAGATGAATTGCCTTTTTATCTTCTTCCTTTTTTTTTTTTTTTCTTTCTTATGTTCTTCGTTGATAAATATTATAGTTTCTTAGGAGTGACGTGAGAAGTAAAAACGAGTATAATTCAGTTTTAGCTCAATAGTAATCACTAATAGTCTTCTATTCTCGAGTCAGGAAGTCTAAATTTTCATCTCATATAATTATCATAGGGTTGTAGTTTGAACCTTTTCACTTCATTATACTAAAAAAAAGCTATACCATAAGTAATGTTACTTGTAAATTAATTAAAAGAGG

mRNA sequence

AAATTTTGATGAAAACCTTATCCTCTTTCACTGCTCTCATCCATGGCGTCCGTCTCTGCTTCGCATAGTGAATACAAGATGTAAGAGACGAGTCGACTGTATTTCAGTTTCATAGACAAACCTTAATGGCCGCCATTGCCGGATCTCTCAACCTTCCTCTTGCGCCTTCAAACCTTCCATTACTCCGCATTAAGAAAAATTGTAAATTCTCACTTCATATAAAAAGCAACGAAAGAAACTGTTTCCCAAATTTAAGACCAACCATTAACCAAAACCAAAACCAAACTCACACAAACCGCCCCACAAGTCTAAATGCAACATTGAGGCGTCCGAAGGGATTCGGACCAGCTTCAAGGAAGAAGAAGACGAAGAAAACAAGAAGAGAAGGAAGTGAGGATGATGACAACGAGGAGGAGGAAGAGGAAGACGAGGAGGAGGAAGAGGAAGGTGGTGTAATACCAGAAGTAGTGACAAATAGGATGATGAGTAGAATGGGATTTACAGTGGGGATTCCATTGTTTATAGGGCTATTGTTCTTCCCATTCTTCTATTACCTCAAAGTTGGGCTGAAGATTGATGTGCCCTCATGGGTGCCAGTCATAGTCTCATTCTTCTTCTTTGGTTCAGCTCTTTTGGGAGTTAGCTATGGGATTGTGTCCTCTAGTTGGGATCCTTTGAGGGACGGCTCTTTATTGGGTTGGAATGAAGCTCAGAAGAATTGGCCTGTTTTTTGGAAGTCCATTTGGGGTGGATCTAATAAGAAATAGATGAATTGCCTTTTTATCTTCTTCCTTTTTTTTTTTTTTTCTTTCTTATGTTCTTCGTTGATAAATATTATAGTTTCTTAGGAGTGACGTGAGAAGTAAAAACGAGTATAATTCAGTTTTAGCTCAATAGTAATCACTAATAGTCTTCTATTCTCGAGTCAGGAAGTCTAAATTTTCATCTCATATAATTATCATAGGGTTGTAGTTTGAACCTTTTCACTTCATTATACTAAAAAAAAGCTATACCATAAGTAATGTTACTTGTAAATTAATTAAAAGAGG

Coding sequence (CDS)

ATGGCCGCCATTGCCGGATCTCTCAACCTTCCTCTTGCGCCTTCAAACCTTCCATTACTCCGCATTAAGAAAAATTGTAAATTCTCACTTCATATAAAAAGCAACGAAAGAAACTGTTTCCCAAATTTAAGACCAACCATTAACCAAAACCAAAACCAAACTCACACAAACCGCCCCACAAGTCTAAATGCAACATTGAGGCGTCCGAAGGGATTCGGACCAGCTTCAAGGAAGAAGAAGACGAAGAAAACAAGAAGAGAAGGAAGTGAGGATGATGACAACGAGGAGGAGGAAGAGGAAGACGAGGAGGAGGAAGAGGAAGGTGGTGTAATACCAGAAGTAGTGACAAATAGGATGATGAGTAGAATGGGATTTACAGTGGGGATTCCATTGTTTATAGGGCTATTGTTCTTCCCATTCTTCTATTACCTCAAAGTTGGGCTGAAGATTGATGTGCCCTCATGGGTGCCAGTCATAGTCTCATTCTTCTTCTTTGGTTCAGCTCTTTTGGGAGTTAGCTATGGGATTGTGTCCTCTAGTTGGGATCCTTTGAGGGACGGCTCTTTATTGGGTTGGAATGAAGCTCAGAAGAATTGGCCTGTTTTTTGGAAGTCCATTTGGGGTGGATCTAATAAGAAATAG

Protein sequence

MAAIAGSLNLPLAPSNLPLLRIKKNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRPTSLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEEEEEEGGVIPEVVTNRMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSSWDPLRDGSLLGWNEAQKNWPVFWKSIWGGSNKK*
Homology
BLAST of CSPI03G01080 vs. ExPASy Swiss-Prot
Match: O49668 (Protein PAM68, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAM68 PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.1e-57
Identity = 115/165 (69.70%), Postives = 140/165 (84.85%), Query Frame = 0

Query: 52  NQTHTNRPTSLNATLRRPKGFGPASRK-KKTKKTR--REGSEDDDNEEEEEEDEEEEEEG 111
           N+   +R   L+AT+  PKGFGP  +K KK+KK +   +  EDDD+E+E+++DEE+E E 
Sbjct: 50  NRLEISRIAPLHATMNSPKGFGPPPKKTKKSKKPKPGNQSDEDDDDEDEDDDDEEDERER 109

Query: 112 GVIPEVVTNRMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSA 171
           GVIPE+VTNRM+SRMGFTVG+PLFIGLLFFPFFYYLKVGLK+DVP+WVP IVSF FFG+A
Sbjct: 110 GVIPEIVTNRMISRMGFTVGLPLFIGLLFFPFFYYLKVGLKVDVPTWVPFIVSFVFFGTA 169

Query: 172 LLGVSYGIVSSSWDPLRDGSLLGWNEAQKNWPVFWKSIWGGSNKK 214
           L GVSYGIVSSSWDPLR+GSLLGWNEA+KNWPVFW+S W  S+K+
Sbjct: 170 LAGVSYGIVSSSWDPLREGSLLGWNEAKKNWPVFWQSFWNSSDKR 214

BLAST of CSPI03G01080 vs. ExPASy Swiss-Prot
Match: Q9LTD9 (Uncharacterized protein PAM68-like OS=Arabidopsis thaliana OX=3702 GN=At5g52780 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.2e-09
Identity = 47/147 (31.97%), Postives = 74/147 (50.34%), Query Frame = 0

Query: 60  TSLNATLRRPKGFGPASRKKKTKKTRR--EGSEDDDNEEEEEEDEEEEEEGGVIPEVVTN 119
           T++      PK   P ++ +   K     +G +     E+    + + E+   IP+ V  
Sbjct: 19  TTVKTKSHNPKTLYPNNKPRWESKLHAGPKGFQSSRTSEKPGRPDPDPEDDPPIPQEVFE 78

Query: 120 RMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIV 179
           RMM R+  +VG PL +G+        LK     DVP WVP + +   FGS+ LG++YG +
Sbjct: 79  RMMGRIVVSVGTPLGLGVAILKVLEVLKDRNVWDVPLWVPYLTTLVTFGSSALGIAYGSL 138

Query: 180 SSSWDPLRDGSLLGWNEAQKNWPVFWK 205
           S++ DP +  SL G  EA++NW   WK
Sbjct: 139 STNLDPAKTNSLFGLKEAKENWVEMWK 165

BLAST of CSPI03G01080 vs. ExPASy TrEMBL
Match: A0A0A0L507 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G006590 PE=4 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 4.7e-112
Identity = 212/213 (99.53%), Postives = 213/213 (100.00%), Query Frame = 0

Query: 1   MAAIAGSLNLPLAPSNLPLLRIKKNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRPT 60
           MAAIAGSLNLPLAPSNLPLLRIKKNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRPT
Sbjct: 1   MAAIAGSLNLPLAPSNLPLLRIKKNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRPT 60

Query: 61  SLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEEEEEEGGVIPEVVTNRMM 120
           SLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEEEEEEGGVIPEVVTNRMM
Sbjct: 61  SLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEEEEEEGGVIPEVVTNRMM 120

Query: 121 SRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSS 180
           SRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSS
Sbjct: 121 SRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSS 180

Query: 181 WDPLRDGSLLGWNEAQKNWPVFWKSIWGGSNKK 214
           WDPLR+GSLLGWNEAQKNWPVFWKSIWGGSNKK
Sbjct: 181 WDPLREGSLLGWNEAQKNWPVFWKSIWGGSNKK 213

BLAST of CSPI03G01080 vs. ExPASy TrEMBL
Match: A0A1S3BKY8 (protein PAM68, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490730 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 6.0e-99
Identity = 194/218 (88.99%), Postives = 202/218 (92.66%), Query Frame = 0

Query: 1   MAAIAGSLNLPLAPSNLPLLRIKKNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRPT 60
           MAAIAGSLNLPLAPSNLPLLRIKKNC+FS+HIKS ERNCFPN RPTI  NQNQT TN PT
Sbjct: 1   MAAIAGSLNLPLAPSNLPLLRIKKNCEFSIHIKSYERNCFPNFRPTI--NQNQTRTNHPT 60

Query: 61  SLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEE-----EEEEGGVIPEVV 120
           SLNATLRRPKGFGPA RKKK KKT+ EG EDDDN+E+EEED E     EEEEGGVIPEVV
Sbjct: 61  SLNATLRRPKGFGPAPRKKKMKKTKGEGREDDDNDEDEEEDNEEEEDNEEEEGGVIPEVV 120

Query: 121 TNRMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYG 180
           TNRMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYG
Sbjct: 121 TNRMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYG 180

Query: 181 IVSSSWDPLRDGSLLGWNEAQKNWPVFWKSIWGGSNKK 214
           IVSSSWDP+R+GSLLGWNEAQKNWPVFW+SIWGGSNKK
Sbjct: 181 IVSSSWDPMREGSLLGWNEAQKNWPVFWQSIWGGSNKK 216

BLAST of CSPI03G01080 vs. ExPASy TrEMBL
Match: A0A1S3BKQ0 (protein PAM68, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490730 PE=4 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 1.5e-97
Identity = 194/219 (88.58%), Postives = 202/219 (92.24%), Query Frame = 0

Query: 1   MAAIAGSLNLPLAPSNLPLLRIK-KNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRP 60
           MAAIAGSLNLPLAPSNLPLLRIK KNC+FS+HIKS ERNCFPN RPTI  NQNQT TN P
Sbjct: 1   MAAIAGSLNLPLAPSNLPLLRIKQKNCEFSIHIKSYERNCFPNFRPTI--NQNQTRTNHP 60

Query: 61  TSLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEE-----EEEEGGVIPEV 120
           TSLNATLRRPKGFGPA RKKK KKT+ EG EDDDN+E+EEED E     EEEEGGVIPEV
Sbjct: 61  TSLNATLRRPKGFGPAPRKKKMKKTKGEGREDDDNDEDEEEDNEEEEDNEEEEGGVIPEV 120

Query: 121 VTNRMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSY 180
           VTNRMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSY
Sbjct: 121 VTNRMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSY 180

Query: 181 GIVSSSWDPLRDGSLLGWNEAQKNWPVFWKSIWGGSNKK 214
           GIVSSSWDP+R+GSLLGWNEAQKNWPVFW+SIWGGSNKK
Sbjct: 181 GIVSSSWDPMREGSLLGWNEAQKNWPVFWQSIWGGSNKK 217

BLAST of CSPI03G01080 vs. ExPASy TrEMBL
Match: A0A6J1CUC6 (protein PAM68, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111014847 PE=4 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 3.3e-81
Identity = 165/213 (77.46%), Postives = 186/213 (87.32%), Query Frame = 0

Query: 1   MAAIAGSLNLPLAPSNLPLLRIKKNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRPT 60
           MAAIAGSLNLPLAPSNLP LRIK+NCKFS+H KS+ER  FP LRP+I   QNQT  N+P 
Sbjct: 1   MAAIAGSLNLPLAPSNLPSLRIKRNCKFSIHSKSHERIRFPLLRPSI--YQNQTRINQPA 60

Query: 61  SLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEEEEEEGGVIPEVVTNRMM 120
            L ATLR PKGFGPA +K+K K    + S+++D +E+E+EDE+EE EGGVIPEVVTNRMM
Sbjct: 61  ILRATLRSPKGFGPAPKKRKKKS---QESDEEDEDEDEDEDEDEEAEGGVIPEVVTNRMM 120

Query: 121 SRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSS 180
           SRMGF+VGIPL IGLLFFPFFYYLKVGLKIDVP+WVP IVSFFFFGSALLGVSYGIVSSS
Sbjct: 121 SRMGFSVGIPLLIGLLFFPFFYYLKVGLKIDVPTWVPFIVSFFFFGSALLGVSYGIVSSS 180

Query: 181 WDPLRDGSLLGWNEAQKNWPVFWKSIWGGSNKK 214
           WDP+R+GSLLGWNEAQKNWPVFW+SIW  S+KK
Sbjct: 181 WDPMREGSLLGWNEAQKNWPVFWQSIWAKSDKK 208

BLAST of CSPI03G01080 vs. ExPASy TrEMBL
Match: A0A6J1GB37 (protein PAM68, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111452558 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 6.4e-77
Identity = 161/214 (75.23%), Postives = 181/214 (84.58%), Query Frame = 0

Query: 1   MAAIAGSLNLPLAPSNLPLLRIKKNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRPT 60
           MAAI GSLNLPLAPS LP L I +N   S+  KS +RN F  LRP++   QNQT TN   
Sbjct: 1   MAAITGSLNLPLAPSKLPSLHITRNYNLSIQSKSQKRNYFSLLRPSL--YQNQTRTNHTL 60

Query: 61  SLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEEEEEEGGVIPEVVTNRMM 120
            +NATLR PKGFGPA RKKK KKT+  G  ++++E+EE+E+EEEEE+ GVIPEVVTNRM+
Sbjct: 61  IVNATLRSPKGFGPAPRKKKKKKTKGGGDGEEESEDEEDEEEEEEEDRGVIPEVVTNRMI 120

Query: 121 SRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSS 180
           SRMGF+VGIPL IGLLFFPFFYYLKV LKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSS
Sbjct: 121 SRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSS 180

Query: 181 WDPLRDGSLLGWNEAQKNWPVFWKSIW-GGSNKK 214
           WDP+R+GSLLGWNEAQKNWPVFW+SIW GGSNKK
Sbjct: 181 WDPMREGSLLGWNEAQKNWPVFWQSIWSGGSNKK 212

BLAST of CSPI03G01080 vs. NCBI nr
Match: XP_004146119.1 (protein PAM68, chloroplastic isoform X2 [Cucumis sativus] >KGN55697.1 hypothetical protein Csa_010628 [Cucumis sativus])

HSP 1 Score: 413.7 bits (1062), Expect = 9.7e-112
Identity = 212/213 (99.53%), Postives = 213/213 (100.00%), Query Frame = 0

Query: 1   MAAIAGSLNLPLAPSNLPLLRIKKNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRPT 60
           MAAIAGSLNLPLAPSNLPLLRIKKNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRPT
Sbjct: 1   MAAIAGSLNLPLAPSNLPLLRIKKNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRPT 60

Query: 61  SLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEEEEEEGGVIPEVVTNRMM 120
           SLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEEEEEEGGVIPEVVTNRMM
Sbjct: 61  SLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEEEEEEGGVIPEVVTNRMM 120

Query: 121 SRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSS 180
           SRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSS
Sbjct: 121 SRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSS 180

Query: 181 WDPLRDGSLLGWNEAQKNWPVFWKSIWGGSNKK 214
           WDPLR+GSLLGWNEAQKNWPVFWKSIWGGSNKK
Sbjct: 181 WDPLREGSLLGWNEAQKNWPVFWKSIWGGSNKK 213

BLAST of CSPI03G01080 vs. NCBI nr
Match: XP_011650314.1 (protein PAM68, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 409.1 bits (1050), Expect = 2.4e-110
Identity = 212/214 (99.07%), Postives = 213/214 (99.53%), Query Frame = 0

Query: 1   MAAIAGSLNLPLAPSNLPLLRIK-KNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRP 60
           MAAIAGSLNLPLAPSNLPLLRIK KNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRP
Sbjct: 1   MAAIAGSLNLPLAPSNLPLLRIKQKNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRP 60

Query: 61  TSLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEEEEEEGGVIPEVVTNRM 120
           TSLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEEEEEEGGVIPEVVTNRM
Sbjct: 61  TSLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEEEEEEGGVIPEVVTNRM 120

Query: 121 MSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSS 180
           MSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSS
Sbjct: 121 MSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSS 180

Query: 181 SWDPLRDGSLLGWNEAQKNWPVFWKSIWGGSNKK 214
           SWDPLR+GSLLGWNEAQKNWPVFWKSIWGGSNKK
Sbjct: 181 SWDPLREGSLLGWNEAQKNWPVFWKSIWGGSNKK 214

BLAST of CSPI03G01080 vs. NCBI nr
Match: XP_008448605.1 (PREDICTED: protein PAM68, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 370.2 bits (949), Expect = 1.2e-98
Identity = 194/218 (88.99%), Postives = 202/218 (92.66%), Query Frame = 0

Query: 1   MAAIAGSLNLPLAPSNLPLLRIKKNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRPT 60
           MAAIAGSLNLPLAPSNLPLLRIKKNC+FS+HIKS ERNCFPN RPTI  NQNQT TN PT
Sbjct: 1   MAAIAGSLNLPLAPSNLPLLRIKKNCEFSIHIKSYERNCFPNFRPTI--NQNQTRTNHPT 60

Query: 61  SLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEE-----EEEEGGVIPEVV 120
           SLNATLRRPKGFGPA RKKK KKT+ EG EDDDN+E+EEED E     EEEEGGVIPEVV
Sbjct: 61  SLNATLRRPKGFGPAPRKKKMKKTKGEGREDDDNDEDEEEDNEEEEDNEEEEGGVIPEVV 120

Query: 121 TNRMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYG 180
           TNRMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYG
Sbjct: 121 TNRMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYG 180

Query: 181 IVSSSWDPLRDGSLLGWNEAQKNWPVFWKSIWGGSNKK 214
           IVSSSWDP+R+GSLLGWNEAQKNWPVFW+SIWGGSNKK
Sbjct: 181 IVSSSWDPMREGSLLGWNEAQKNWPVFWQSIWGGSNKK 216

BLAST of CSPI03G01080 vs. NCBI nr
Match: XP_008448604.1 (PREDICTED: protein PAM68, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 365.5 bits (937), Expect = 3.0e-97
Identity = 194/219 (88.58%), Postives = 202/219 (92.24%), Query Frame = 0

Query: 1   MAAIAGSLNLPLAPSNLPLLRIK-KNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRP 60
           MAAIAGSLNLPLAPSNLPLLRIK KNC+FS+HIKS ERNCFPN RPTI  NQNQT TN P
Sbjct: 1   MAAIAGSLNLPLAPSNLPLLRIKQKNCEFSIHIKSYERNCFPNFRPTI--NQNQTRTNHP 60

Query: 61  TSLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEE-----EEEEGGVIPEV 120
           TSLNATLRRPKGFGPA RKKK KKT+ EG EDDDN+E+EEED E     EEEEGGVIPEV
Sbjct: 61  TSLNATLRRPKGFGPAPRKKKMKKTKGEGREDDDNDEDEEEDNEEEEDNEEEEGGVIPEV 120

Query: 121 VTNRMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSY 180
           VTNRMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSY
Sbjct: 121 VTNRMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSY 180

Query: 181 GIVSSSWDPLRDGSLLGWNEAQKNWPVFWKSIWGGSNKK 214
           GIVSSSWDP+R+GSLLGWNEAQKNWPVFW+SIWGGSNKK
Sbjct: 181 GIVSSSWDPMREGSLLGWNEAQKNWPVFWQSIWGGSNKK 217

BLAST of CSPI03G01080 vs. NCBI nr
Match: XP_038877162.1 (protein PAM68, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 327.8 bits (839), Expect = 7.0e-86
Identity = 171/213 (80.28%), Postives = 191/213 (89.67%), Query Frame = 0

Query: 1   MAAIAGSLNLPLAPSNLPLLRIKKNCKFSLHIKSNERNCFPNLRPTINQNQNQTHTNRPT 60
           MAAI+GSLNLPLAPSNL   RIK+NCK S+H+KS ERNCFP L P+I  NQ + HTN+ T
Sbjct: 1   MAAISGSLNLPLAPSNLSSPRIKRNCKISIHLKSYERNCFPKLIPSI--NQRRIHTNQST 60

Query: 61  SLNATLRRPKGFGPASRKKKTKKTRREGSEDDDNEEEEEEDEEEEEEGGVIPEVVTNRMM 120
           +LNATLRR KGFGPA R+KK KKT+REGSED+  +E+E   +EEEEEGGVIPEVVTNRM+
Sbjct: 61  TLNATLRRQKGFGPAPRRKKMKKTKREGSEDEHGDEDE---DEEEEEGGVIPEVVTNRMI 120

Query: 121 SRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSS 180
           SRMGF+VGIPL IGLLFFPFFYYLKV LKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSS
Sbjct: 121 SRMGFSVGIPLLIGLLFFPFFYYLKVVLKIDVPSWVPVIVSFFFFGSALLGVSYGIVSSS 180

Query: 181 WDPLRDGSLLGWNEAQKNWPVFWKSIWGGSNKK 214
           WDP+R+GSLLGWNEAQKNWPVFW+S+WGGSNKK
Sbjct: 181 WDPMREGSLLGWNEAQKNWPVFWQSLWGGSNKK 208

BLAST of CSPI03G01080 vs. TAIR 10
Match: AT4G19100.1 (Protein of unknown function (DUF3464) )

HSP 1 Score: 224.6 bits (571), Expect = 7.8e-59
Identity = 115/165 (69.70%), Postives = 140/165 (84.85%), Query Frame = 0

Query: 52  NQTHTNRPTSLNATLRRPKGFGPASRK-KKTKKTR--REGSEDDDNEEEEEEDEEEEEEG 111
           N+   +R   L+AT+  PKGFGP  +K KK+KK +   +  EDDD+E+E+++DEE+E E 
Sbjct: 50  NRLEISRIAPLHATMNSPKGFGPPPKKTKKSKKPKPGNQSDEDDDDEDEDDDDEEDERER 109

Query: 112 GVIPEVVTNRMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSA 171
           GVIPE+VTNRM+SRMGFTVG+PLFIGLLFFPFFYYLKVGLK+DVP+WVP IVSF FFG+A
Sbjct: 110 GVIPEIVTNRMISRMGFTVGLPLFIGLLFFPFFYYLKVGLKVDVPTWVPFIVSFVFFGTA 169

Query: 172 LLGVSYGIVSSSWDPLRDGSLLGWNEAQKNWPVFWKSIWGGSNKK 214
           L GVSYGIVSSSWDPLR+GSLLGWNEA+KNWPVFW+S W  S+K+
Sbjct: 170 LAGVSYGIVSSSWDPLREGSLLGWNEAKKNWPVFWQSFWNSSDKR 214

BLAST of CSPI03G01080 vs. TAIR 10
Match: AT5G52780.1 (Protein of unknown function (DUF3464) )

HSP 1 Score: 63.5 bits (153), Expect = 2.3e-10
Identity = 47/147 (31.97%), Postives = 74/147 (50.34%), Query Frame = 0

Query: 60  TSLNATLRRPKGFGPASRKKKTKKTRR--EGSEDDDNEEEEEEDEEEEEEGGVIPEVVTN 119
           T++      PK   P ++ +   K     +G +     E+    + + E+   IP+ V  
Sbjct: 19  TTVKTKSHNPKTLYPNNKPRWESKLHAGPKGFQSSRTSEKPGRPDPDPEDDPPIPQEVFE 78

Query: 120 RMMSRMGFTVGIPLFIGLLFFPFFYYLKVGLKIDVPSWVPVIVSFFFFGSALLGVSYGIV 179
           RMM R+  +VG PL +G+        LK     DVP WVP + +   FGS+ LG++YG +
Sbjct: 79  RMMGRIVVSVGTPLGLGVAILKVLEVLKDRNVWDVPLWVPYLTTLVTFGSSALGIAYGSL 138

Query: 180 SSSWDPLRDGSLLGWNEAQKNWPVFWK 205
           S++ DP +  SL G  EA++NW   WK
Sbjct: 139 STNLDPAKTNSLFGLKEAKENWVEMWK 165

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O496681.1e-5769.70Protein PAM68, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAM68 PE=1 SV=1[more]
Q9LTD93.2e-0931.97Uncharacterized protein PAM68-like OS=Arabidopsis thaliana OX=3702 GN=At5g52780 ... [more]
Match NameE-valueIdentityDescription
A0A0A0L5074.7e-11299.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G006590 PE=4 SV=1[more]
A0A1S3BKY86.0e-9988.99protein PAM68, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490730 ... [more]
A0A1S3BKQ01.5e-9788.58protein PAM68, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490730 ... [more]
A0A6J1CUC63.3e-8177.46protein PAM68, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111014847 PE=4... [more]
A0A6J1GB376.4e-7775.23protein PAM68, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111452558 PE=4 ... [more]
Match NameE-valueIdentityDescription
XP_004146119.19.7e-11299.53protein PAM68, chloroplastic isoform X2 [Cucumis sativus] >KGN55697.1 hypothetic... [more]
XP_011650314.12.4e-11099.07protein PAM68, chloroplastic isoform X1 [Cucumis sativus][more]
XP_008448605.11.2e-9888.99PREDICTED: protein PAM68, chloroplastic isoform X2 [Cucumis melo][more]
XP_008448604.13.0e-9788.58PREDICTED: protein PAM68, chloroplastic isoform X1 [Cucumis melo][more]
XP_038877162.17.0e-8680.28protein PAM68, chloroplastic isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G19100.17.8e-5969.70Protein of unknown function (DUF3464) [more]
AT5G52780.12.3e-1031.97Protein of unknown function (DUF3464) [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 87..107
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 40..113
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 40..65
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 92..111
NoneNo IPR availablePANTHERPTHR34575:SF1PROTEIN PAM68, CHLOROPLASTICcoord: 27..213
IPR021855PAM68-likePFAMPF11947DUF3464coord: 68..206
e-value: 3.9E-51
score: 172.7
IPR021855PAM68-likePANTHERPTHR34575PROTEIN PAM68, CHLOROPLASTICcoord: 27..213

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G01080.1CSPI03G01080.1mRNA
CSPI03G01080.2CSPI03G01080.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010207 photosystem II assembly
cellular_component GO:0016021 integral component of membrane