CSPI02G11580 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G11580
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationChr2: 11915051 .. 11916655 (-)
RNA-Seq ExpressionCSPI02G11580
SyntenyCSPI02G11580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGCATTTGAAAACTGGTTATTTGAGTTGATTGGCGTAATAACTCTAAAAATGGTGGGTTATAGTTATAGTTTGGCAATTTGTTTGCTATACATGTAATTAACAATGGAAAACTACTTGCCACCAATAATTTCTCCCAAGGCCCTAACAACCCACCCTTTTTAAGTCTACAAGTTAGAACTCATAAAAATCAAAGCCATAAGAAAGAGATATTCATTTATTGGCATAAAAAAGAGAGAGAAATTTCTCTGTTAATTATATCCTTCTCTTCATCTGCCGCTTTTTTCTCCTTTCATCTCTACTCTCTCCACTCTCCAATGGCTTCCACTGAAATTGCCTACAACCTTTCCCCTATGCTCATCGTTTATAAAGACGGTCGCGCCGAAAGGCTCGTCGGCAACGAATTAGTCCATCCCTCTCTTGACCCTTTAACAGTCGTTGAGTCCAAAGACATCGTCATCTCGCCAGAAACCCCCGTCTCTGCTCGGATTTACAGGCCCAAACCCACCGCAGAACCTCACAAGCTTCCTCTTCTCATTTATATTCACGGCGGCGGCTTCTGCATCGAATCAGCATTCTCTCCTACCTACCACCACCATCTCAACTCCTTAGTCGCTGAAGCCAACGTCATCGCTATCTCCGTCGAGTACCGGCGAGCTCCGGAGCACCCACTTCCGGTCGCTTATGAAGATTCCTGGACTGCTTTGAAATGGGTTGCCGCCCACTCGGCTGGAACCGGCCCAGAGGAGTGGTTGAATAAAATTGCAGATTTTAACCGGGTTTATTTCGCCGGAGATAGCGCTGGAGCCAACGTCGCTAATAAAATGGCGATCCGGGTCGGAATGGAAGGGGTGGCCGGGTTGAATCTGAAGGGATTGATGTTGGTTCATCCTTATTTCTGGGGAGAGAAATTGATCGGAGATGAAGAGAAGCTGAAGCCGGAAGAAAGATGGTTTATTGAGAAATTGTGGTATGTGGCTTGTCCTACTATTAGCGGGTTAGATGACCCGATTGTGAACCCGGAATTTGAACCGAATTTAGGGAAGGTAACGGCGGAGAGAGTGGCGGTTTATGTGGCGGAGAAGGATGCTCTGAAAGACAGAGGACGGTTTTACAGTGAGTGTTTGAAGAAATCGGGATGGGGGGGCGCCGTCGAGGTGATGGAGACGAAAGGCCAAGGCCACGTGTTTCATTTGTTCAACCCTACGTCAGACGATACGGTTCAATTCGTCGGAAAGCTAGCGGCCTTCCTCAACGGCGGACCCTGAGATTGAGCTGATAGGAAGGATGAAATGAAAAATTCCCCTCCACACCTCCTTCTTCGGCGGTGGAATTTGAAGCAGTTTTCATATAAATTTCCTTTTTTTTTTGTTGTTTTCAATGATTTTGTAATTTGGAGGGGAAAAAGAATAAAGGGATTGTGAAAGTGAAATAAGTTTTTTTCTCTGAATTTAGGATTTGCTTTTTCTTCGTTTACTATGGTTGGTAGAATGTGTAGTCTAATAATGAACTTTGTTTTCTTATATACAAAGGGAGTGGATTGAATTATTCACTTCTATTTTATAAAATCATATTAATTATAAATTATAGTTCAGCTAAATTACTC

mRNA sequence

AAGCATTTGAAAACTGGTTATTTGAGTTGATTGGCGTAATAACTCTAAAAATGGTGGGTTATAGTTATAGTTTGGCAATTTGTTTGCTATACATGTAATTAACAATGGAAAACTACTTGCCACCAATAATTTCTCCCAAGGCCCTAACAACCCACCCTTTTTAAGTCTACAAGTTAGAACTCATAAAAATCAAAGCCATAAGAAAGAGATATTCATTTATTGGCATAAAAAAGAGAGAGAAATTTCTCTGTTAATTATATCCTTCTCTTCATCTGCCGCTTTTTTCTCCTTTCATCTCTACTCTCTCCACTCTCCAATGGCTTCCACTGAAATTGCCTACAACCTTTCCCCTATGCTCATCGTTTATAAAGACGGTCGCGCCGAAAGGCTCGTCGGCAACGAATTAGTCCATCCCTCTCTTGACCCTTTAACAGTCGTTGAGTCCAAAGACATCGTCATCTCGCCAGAAACCCCCGTCTCTGCTCGGATTTACAGGCCCAAACCCACCGCAGAACCTCACAAGCTTCCTCTTCTCATTTATATTCACGGCGGCGGCTTCTGCATCGAATCAGCATTCTCTCCTACCTACCACCACCATCTCAACTCCTTAGTCGCTGAAGCCAACGTCATCGCTATCTCCGTCGAGTACCGGCGAGCTCCGGAGCACCCACTTCCGGTCGCTTATGAAGATTCCTGGACTGCTTTGAAATGGGTTGCCGCCCACTCGGCTGGAACCGGCCCAGAGGAGTGGTTGAATAAAATTGCAGATTTTAACCGGGTTTATTTCGCCGGAGATAGCGCTGGAGCCAACGTCGCTAATAAAATGGCGATCCGGGTCGGAATGGAAGGGGTGGCCGGGTTGAATCTGAAGGGATTGATGTTGGTTCATCCTTATTTCTGGGGAGAGAAATTGATCGGAGATGAAGAGAAGCTGAAGCCGGAAGAAAGATGGTTTATTGAGAAATTGTGGTATGTGGCTTGTCCTACTATTAGCGGGTTAGATGACCCGATTGTGAACCCGGAATTTGAACCGAATTTAGGGAAGGTAACGGCGGAGAGAGTGGCGGTTTATGTGGCGGAGAAGGATGCTCTGAAAGACAGAGGACGGTTTTACAGTGAGTGTTTGAAGAAATCGGGATGGGGGGGCGCCGTCGAGGTGATGGAGACGAAAGGCCAAGGCCACGTGTTTCATTTGTTCAACCCTACGTCAGACGATACGGTTCAATTCGTCGGAAAGCTAGCGGCCTTCCTCAACGGCGGACCCTGAGATTGAGCTGATAGGAAGGATGAAATGAAAAATTCCCCTCCACACCTCCTTCTTCGGCGGTGGAATTTGAAGCAGTTTTCATATAAATTTCCTTTTTTTTTTGTTGTTTTCAATGATTTTGTAATTTGGAGGGGAAAAAGAATAAAGGGATTGTGAAAGTGAAATAAGTTTTTTTCTCTGAATTTAGGATTTGCTTTTTCTTCGTTTACTATGGTTGGTAGAATGTGTAGTCTAATAATGAACTTTGTTTTCTTATATACAAAGGGAGTGGATTGAATTATTCACTTCTATTTTATAAAATCATATTAATTATAAATTATAGTTCAGCTAAATTACTC

Coding sequence (CDS)

ATGGCTTCCACTGAAATTGCCTACAACCTTTCCCCTATGCTCATCGTTTATAAAGACGGTCGCGCCGAAAGGCTCGTCGGCAACGAATTAGTCCATCCCTCTCTTGACCCTTTAACAGTCGTTGAGTCCAAAGACATCGTCATCTCGCCAGAAACCCCCGTCTCTGCTCGGATTTACAGGCCCAAACCCACCGCAGAACCTCACAAGCTTCCTCTTCTCATTTATATTCACGGCGGCGGCTTCTGCATCGAATCAGCATTCTCTCCTACCTACCACCACCATCTCAACTCCTTAGTCGCTGAAGCCAACGTCATCGCTATCTCCGTCGAGTACCGGCGAGCTCCGGAGCACCCACTTCCGGTCGCTTATGAAGATTCCTGGACTGCTTTGAAATGGGTTGCCGCCCACTCGGCTGGAACCGGCCCAGAGGAGTGGTTGAATAAAATTGCAGATTTTAACCGGGTTTATTTCGCCGGAGATAGCGCTGGAGCCAACGTCGCTAATAAAATGGCGATCCGGGTCGGAATGGAAGGGGTGGCCGGGTTGAATCTGAAGGGATTGATGTTGGTTCATCCTTATTTCTGGGGAGAGAAATTGATCGGAGATGAAGAGAAGCTGAAGCCGGAAGAAAGATGGTTTATTGAGAAATTGTGGTATGTGGCTTGTCCTACTATTAGCGGGTTAGATGACCCGATTGTGAACCCGGAATTTGAACCGAATTTAGGGAAGGTAACGGCGGAGAGAGTGGCGGTTTATGTGGCGGAGAAGGATGCTCTGAAAGACAGAGGACGGTTTTACAGTGAGTGTTTGAAGAAATCGGGATGGGGGGGCGCCGTCGAGGTGATGGAGACGAAAGGCCAAGGCCACGTGTTTCATTTGTTCAACCCTACGTCAGACGATACGGTTCAATTCGTCGGAAAGCTAGCGGCCTTCCTCAACGGCGGACCCTGA

Protein sequence

MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYRPKPTAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLPVAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVAGLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPNLGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPTSDDTVQFVGKLAAFLNGGP*
Homology
BLAST of CSPI02G11580 vs. ExPASy Swiss-Prot
Match: Q9SMM9 (Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 1.9e-82
Identity = 160/325 (49.23%), Postives = 217/325 (66.77%), Query Frame = 0

Query: 4   TEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYRPK- 63
           +EIA + SPMLI+YK GR ERLVG   V PS +P   V SKD+V SP+  +S RIY P+ 
Sbjct: 3   SEIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEK 62

Query: 64  -PTAEPH---KLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHP 123
             TAE     KLPLL+Y HGGGF +E+AFSPTYH  L + V+ ++ +A+SV+YRRAPEHP
Sbjct: 63  AATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHP 122

Query: 124 LPVAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEG 183
           +P +Y+DSWTALKWV +H AG+G E+WLNK ADF++V+ AGDSAGAN+ + M ++   + 
Sbjct: 123 IPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDK 182

Query: 184 VA--GLN---LKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACP-TISGLDDPI 243
           ++   LN   + G++LVHPYFW +  + D+E      R +IE +W +A P +  G DDP 
Sbjct: 183 LSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPF 242

Query: 244 VN--PEFEPNLGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAV-EVMETKGQGH 303
           +N       +L  +   +V V VAEKDAL  +G  Y E L KS W G V +V+ETKG+GH
Sbjct: 243 INVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGH 302

Query: 304 VFHLFNPTSDDTVQFVGKLAAFLNG 315
           VFHL +P S+   + V + A F+ G
Sbjct: 303 VFHLRDPNSEKAHELVHRFAGFIKG 327

BLAST of CSPI02G11580 vs. ExPASy Swiss-Prot
Match: Q9SMN0 (Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 5.6e-82
Identity = 154/321 (47.98%), Postives = 213/321 (66.36%), Query Frame = 0

Query: 4   TEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYRPKP 63
           +EIA + SP+L +YK GR ERL+G   V PS +P   V SKD+V S +  +S RIY P+ 
Sbjct: 3   SEIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEK 62

Query: 64  TA--EPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLPV 123
            A     KLPLL+Y HGGGF IE+AFSPTYH  L + V+ +N +A+SV+YRRAPEHP+ V
Sbjct: 63  AAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPISV 122

Query: 124 AYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA- 183
            ++DSWTALKWV  H  G+G E+WLNK ADF+RV+ +GDSAGAN+ + MA+R   E ++ 
Sbjct: 123 PFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSP 182

Query: 184 GLN---LKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACP-TISGLDDPIVN-- 243
           GLN   + G++L+HPYFW +  I +++      R  IE  W +A P +  G DDP++N  
Sbjct: 183 GLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVV 242

Query: 244 PEFEPNLGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLF 303
                +L  +   +V V VAEKDAL  +G  Y+  L+KSGW G VEV+E++G+ HVFHL 
Sbjct: 243 QSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLL 302

Query: 304 NPTSDDTVQFVGKLAAFLNGG 316
            P  D+ ++ + K + F+ GG
Sbjct: 303 KPECDNAIEVMHKFSGFIKGG 323

BLAST of CSPI02G11580 vs. ExPASy Swiss-Prot
Match: Q9ZQ91 (Probable carboxylesterase 7 OS=Arabidopsis thaliana OX=3702 GN=CXE7 PE=2 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.0e-80
Identity = 158/309 (51.13%), Postives = 196/309 (63.43%), Query Frame = 0

Query: 6   IAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYRPKPTA 65
           IA++ SPM  VYK GR ERL+G   V PSL P   V SKDI+ SPE  +S RIY P+   
Sbjct: 5   IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKVT 64

Query: 66  EPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLPVAYED 125
              KLP+LIY HGGGF IE+AFSP YH  L S VA AN +AISV YRRAPE P+P+ YED
Sbjct: 65  -VKKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPIPYED 124

Query: 126 SWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVAGLNLK 185
           SW +LKWV  H  GTGPE W+NK  DF +V+ AGDSAG N+++ + +R   E +    + 
Sbjct: 125 SWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCDSLIS 184

Query: 186 GLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACP-TISGLDDPIVNPEFEPNLGKV 245
           G++L+HPYFW +  I + E     +   +E  W VA P +  G+DDP +N       G +
Sbjct: 185 GIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDPSG-L 244

Query: 246 TAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPTSDDTVQF 305
              RV V VA  D    +G  Y+E LKKSGW G VEVMETK +GHVFHL NP SD+  Q 
Sbjct: 245 GCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNARQV 304

Query: 306 VGKLAAFLN 314
           V KL  F+N
Sbjct: 305 VKKLEEFIN 311

BLAST of CSPI02G11580 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.4e-80
Identity = 151/312 (48.40%), Postives = 202/312 (64.74%), Query Frame = 0

Query: 5   EIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYRPKPT 64
           +++  L P L+V+ DG  ERL G E+  P LDP+T V SKDI+I P+T +SARIYRP   
Sbjct: 7   QVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIYRPFSI 66

Query: 65  AEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLPVAYE 124
               K+PL++Y HGG F I S   P+YH  LN +V +ANVIA+SV YR APEHPLP AYE
Sbjct: 67  QPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLPTAYE 126

Query: 125 DSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVAGLNL 184
           DSWTALK + A +     E W+N  AD + ++  GDSAGAN+++ +A R   +    L +
Sbjct: 127 DSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFR-AKQSDQTLKI 186

Query: 185 KGLMLVHPYFWGEKLIGDEEKLKPEER-WFIEKLWYVACPTISGLDDPIVNP--EFEPNL 244
           KG+ ++HPYFWG + IG E  +K E R   ++  W   CP+  G DDP +NP  +  P+L
Sbjct: 187 KGIGMIHPYFWGTQPIGAE--IKDEARKQMVDGWWEFVCPSEKGSDDPWINPFADGSPDL 246

Query: 245 GKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPTSDDT 304
           G +  ERV + VAEKD L +RG+ Y E L KS W G VE+METK + HVFH+F P  D+ 
Sbjct: 247 GGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDCDEA 306

Query: 305 VQFVGKLAAFLN 314
           ++ V  LA F+N
Sbjct: 307 MEMVRCLALFIN 310

BLAST of CSPI02G11580 vs. ExPASy Swiss-Prot
Match: Q9FX94 (Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 7.0e-77
Identity = 141/317 (44.48%), Postives = 203/317 (64.04%), Query Frame = 0

Query: 4   TEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYRPKP 63
           +EIA    P   +YKDGR ERL+G + +  SLDP   V SKD++ SPE  +S R++ P  
Sbjct: 3   SEIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHK 62

Query: 64  TAE---PHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLP 123
           + +    +KLPLLIYIHGG + IES FSP YH++L  +V  AN +A+SV+YRRAPE P+P
Sbjct: 63  STKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVP 122

Query: 124 VAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA 183
            AYED W+A++W+ AHS G+GP +W+NK ADF +V+  GDSAG N+++ MA++ G E   
Sbjct: 123 AAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKL 182

Query: 184 GLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLW-YVACP-TISGLDDPIVNPEFE 243
            L +KG+ +VHP FWG   + + +    E R  I ++W  +A P +++G DDP+ N    
Sbjct: 183 DLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGS 242

Query: 244 -PNLGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPT 303
             +   +  ++V V VA KD    +G  Y+  L+K  W G VEV+E +G+ HVFHL NP 
Sbjct: 243 GSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPK 302

Query: 304 SDDTVQFVGKLAAFLNG 315
           SD  ++F+ K   F+ G
Sbjct: 303 SDKALKFLKKFVEFIIG 319

BLAST of CSPI02G11580 vs. ExPASy TrEMBL
Match: A0A0A0LIH8 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G234520 PE=4 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 1.8e-184
Identity = 313/316 (99.05%), Postives = 314/316 (99.37%), Query Frame = 0

Query: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60
           MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR
Sbjct: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60

Query: 61  PKPTAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLP 120
           PKPTAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLP
Sbjct: 61  PKPTAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLP 120

Query: 121 VAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA 180
           +AYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA
Sbjct: 121 IAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA 180

Query: 181 GLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPN 240
           GLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPN
Sbjct: 181 GLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPN 240

Query: 241 LGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPTSDD 300
           LGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEV ETKGQGHVFHLFNPTSDD
Sbjct: 241 LGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVTETKGQGHVFHLFNPTSDD 300

Query: 301 TVQFVGKLAAFLNGGP 317
            VQFVGKLAAFLNGGP
Sbjct: 301 AVQFVGKLAAFLNGGP 316

BLAST of CSPI02G11580 vs. ExPASy TrEMBL
Match: A0A5A7SPY3 (Putative carboxylesterase 13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G00320 PE=4 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 2.5e-170
Identity = 284/315 (90.16%), Postives = 302/315 (95.87%), Query Frame = 0

Query: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60
           MASTEIA+N SPM+IVYKDGR ERLVGNEL+ PSLDPLT VESKDI+ISPETPVSARIYR
Sbjct: 1   MASTEIAHNFSPMIIVYKDGRTERLVGNELIDPSLDPLTGVESKDIIISPETPVSARIYR 60

Query: 61  PKPTAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLP 120
           PKPTAEPHKLPLLIY HGGGFCIESAFSPTYH+HLNSLVAEANVIAISVEYRRAPE+PLP
Sbjct: 61  PKPTAEPHKLPLLIYFHGGGFCIESAFSPTYHNHLNSLVAEANVIAISVEYRRAPEYPLP 120

Query: 121 VAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA 180
           +AYED WTALKWVAAHSAGTGPEEWLN+IAD NRVYFAGDSAGAN+ANKMAIRVG+EGVA
Sbjct: 121 IAYEDCWTALKWVAAHSAGTGPEEWLNEIADLNRVYFAGDSAGANIANKMAIRVGIEGVA 180

Query: 181 GLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPN 240
           GLNLKGLML+HPYFWGEKLIGDEEKLKPE+RWFIEKLWY+ACPTISGLDDP+VNPEFEP 
Sbjct: 181 GLNLKGLMLIHPYFWGEKLIGDEEKLKPEDRWFIEKLWYLACPTISGLDDPVVNPEFEPK 240

Query: 241 LGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPTSDD 300
           LG + AERVAVYVAEKD+LKDRG FYSECLKKSGWGGAVEV+ETKGQGHVFHLFNPTSDD
Sbjct: 241 LGGLAAERVAVYVAEKDSLKDRGWFYSECLKKSGWGGAVEVVETKGQGHVFHLFNPTSDD 300

Query: 301 TVQFVGKLAAFLNGG 316
            V FVGKLAAF+NGG
Sbjct: 301 AVDFVGKLAAFINGG 315

BLAST of CSPI02G11580 vs. ExPASy TrEMBL
Match: A0A1S3CAV3 (probable carboxylesterase 13 OS=Cucumis melo OX=3656 GN=LOC103498400 PE=4 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 2.5e-170
Identity = 284/315 (90.16%), Postives = 302/315 (95.87%), Query Frame = 0

Query: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60
           MASTEIA+N SPM+IVYKDGR ERLVGNEL+ PSLDPLT VESKDI+ISPETPVSARIYR
Sbjct: 1   MASTEIAHNFSPMIIVYKDGRTERLVGNELIDPSLDPLTGVESKDIIISPETPVSARIYR 60

Query: 61  PKPTAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLP 120
           PKPTAEPHKLPLLIY HGGGFCIESAFSPTYH+HLNSLVAEANVIAISVEYRRAPE+PLP
Sbjct: 61  PKPTAEPHKLPLLIYFHGGGFCIESAFSPTYHNHLNSLVAEANVIAISVEYRRAPEYPLP 120

Query: 121 VAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA 180
           +AYED WTALKWVAAHSAGTGPEEWLN+IAD NRVYFAGDSAGAN+ANKMAIRVG+EGVA
Sbjct: 121 IAYEDCWTALKWVAAHSAGTGPEEWLNEIADLNRVYFAGDSAGANIANKMAIRVGIEGVA 180

Query: 181 GLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPN 240
           GLNLKGLML+HPYFWGEKLIGDEEKLKPE+RWFIEKLWY+ACPTISGLDDP+VNPEFEP 
Sbjct: 181 GLNLKGLMLIHPYFWGEKLIGDEEKLKPEDRWFIEKLWYLACPTISGLDDPVVNPEFEPK 240

Query: 241 LGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPTSDD 300
           LG + AERVAVYVAEKD+LKDRG FYSECLKKSGWGGAVEV+ETKGQGHVFHLFNPTSDD
Sbjct: 241 LGGLAAERVAVYVAEKDSLKDRGWFYSECLKKSGWGGAVEVVETKGQGHVFHLFNPTSDD 300

Query: 301 TVQFVGKLAAFLNGG 316
            V FVGKLAAF+NGG
Sbjct: 301 AVDFVGKLAAFINGG 315

BLAST of CSPI02G11580 vs. ExPASy TrEMBL
Match: A0A6J1FW98 (probable carboxylesterase 13 OS=Cucurbita moschata OX=3662 GN=LOC111449075 PE=4 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 1.0e-142
Identity = 237/315 (75.24%), Postives = 273/315 (86.67%), Query Frame = 0

Query: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60
           M STEIA+N SP+ IVYKDG   RLVGN+ + PS DP+T V+SKD+VISPET VS RIYR
Sbjct: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60

Query: 61  PKP-TAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPL 120
           PKP TA+P KLP+L+Y+HGGGFCIESAFSP Y  H+ SL AEANV+A+SV+YR APEHPL
Sbjct: 61  PKPATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEHPL 120

Query: 121 PVAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGV 180
           P+AYEDSW ALKWVAAHS G GPEEWLN I D +RVYFAGDSAGAN+ + +A+RVG EG+
Sbjct: 121 PIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGI 180

Query: 181 AGLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEP 240
           AGLNLKGL+LVHPYFWG KL+GDEEKL+PEER F+EKLWYV CPTISGLDDPIVNPE++P
Sbjct: 181 AGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEYDP 240

Query: 241 NLGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPTSD 300
            LG++ AERV VYVAEKDALK+RG FYSECLKKSGWGG VEV+ETKG+GHVFHLFNPT+D
Sbjct: 241 KLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTTD 300

Query: 301 DTVQFVGKLAAFLNG 315
             + FV  LAAF+NG
Sbjct: 301 VALDFVRNLAAFING 315

BLAST of CSPI02G11580 vs. ExPASy TrEMBL
Match: A0A6J1JBI9 (probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111484271 PE=4 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.9e-141
Identity = 236/317 (74.45%), Postives = 272/317 (85.80%), Query Frame = 0

Query: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60
           M STEIA+N SP+ IVYKDG   R +GN+++ PS DPLT V+SKD+VISPET VS RIYR
Sbjct: 1   MDSTEIAHNFSPLFIVYKDGTVHRFLGNDVIPPSPDPLTGVDSKDVVISPETAVSVRIYR 60

Query: 61  PKP---TAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEH 120
           PKP   TA+P KLP+L+Y+HGGGFCIESAFSP Y  H+ SL AEANV+A+SV+YR APEH
Sbjct: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLSAEANVVAVSVDYRLAPEH 120

Query: 121 PLPVAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGME 180
           PLP+AYEDSW ALKWVAAHS G GPEEWLN I D +RVYFAGDSAGAN+ + +A+RVG E
Sbjct: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180

Query: 181 GVAGLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEF 240
           G+ GLNLKGL+LVHPYFWG KL+GDEEKL+PEER F+EKLWYV CPTISGLDD IVNPE+
Sbjct: 181 GIPGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDSIVNPEY 240

Query: 241 EPNLGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPT 300
           +P LG++ AERV VYVAEKDALK+RG FYSECLKKSGWGG VEV+ETKG+GHVFHLFNPT
Sbjct: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300

Query: 301 SDDTVQFVGKLAAFLNG 315
           +D  V FV KLAAF+NG
Sbjct: 301 TDVAVDFVRKLAAFING 317

BLAST of CSPI02G11580 vs. NCBI nr
Match: KAE8651854.1 (hypothetical protein Csa_006663 [Cucumis sativus])

HSP 1 Score: 654.8 bits (1688), Expect = 3.7e-184
Identity = 313/316 (99.05%), Postives = 314/316 (99.37%), Query Frame = 0

Query: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60
           MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR
Sbjct: 95  MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 154

Query: 61  PKPTAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLP 120
           PKPTAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLP
Sbjct: 155 PKPTAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLP 214

Query: 121 VAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA 180
           +AYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA
Sbjct: 215 IAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA 274

Query: 181 GLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPN 240
           GLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPN
Sbjct: 275 GLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPN 334

Query: 241 LGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPTSDD 300
           LGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEV ETKGQGHVFHLFNPTSDD
Sbjct: 335 LGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVTETKGQGHVFHLFNPTSDD 394

Query: 301 TVQFVGKLAAFLNGGP 317
            VQFVGKLAAFLNGGP
Sbjct: 395 AVQFVGKLAAFLNGGP 410

BLAST of CSPI02G11580 vs. NCBI nr
Match: XP_004154254.1 (probable carboxylesterase 13 [Cucumis sativus])

HSP 1 Score: 654.8 bits (1688), Expect = 3.7e-184
Identity = 313/316 (99.05%), Postives = 314/316 (99.37%), Query Frame = 0

Query: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60
           MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR
Sbjct: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60

Query: 61  PKPTAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLP 120
           PKPTAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLP
Sbjct: 61  PKPTAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLP 120

Query: 121 VAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA 180
           +AYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA
Sbjct: 121 IAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA 180

Query: 181 GLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPN 240
           GLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPN
Sbjct: 181 GLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPN 240

Query: 241 LGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPTSDD 300
           LGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEV ETKGQGHVFHLFNPTSDD
Sbjct: 241 LGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVTETKGQGHVFHLFNPTSDD 300

Query: 301 TVQFVGKLAAFLNGGP 317
            VQFVGKLAAFLNGGP
Sbjct: 301 AVQFVGKLAAFLNGGP 316

BLAST of CSPI02G11580 vs. NCBI nr
Match: XP_008459209.2 (PREDICTED: probable carboxylesterase 13 [Cucumis melo] >KAA0032066.1 putative carboxylesterase 13 [Cucumis melo var. makuwa] >TYK13556.1 putative carboxylesterase 13 [Cucumis melo var. makuwa])

HSP 1 Score: 607.8 bits (1566), Expect = 5.2e-170
Identity = 284/315 (90.16%), Postives = 302/315 (95.87%), Query Frame = 0

Query: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60
           MASTEIA+N SPM+IVYKDGR ERLVGNEL+ PSLDPLT VESKDI+ISPETPVSARIYR
Sbjct: 1   MASTEIAHNFSPMIIVYKDGRTERLVGNELIDPSLDPLTGVESKDIIISPETPVSARIYR 60

Query: 61  PKPTAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLP 120
           PKPTAEPHKLPLLIY HGGGFCIESAFSPTYH+HLNSLVAEANVIAISVEYRRAPE+PLP
Sbjct: 61  PKPTAEPHKLPLLIYFHGGGFCIESAFSPTYHNHLNSLVAEANVIAISVEYRRAPEYPLP 120

Query: 121 VAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA 180
           +AYED WTALKWVAAHSAGTGPEEWLN+IAD NRVYFAGDSAGAN+ANKMAIRVG+EGVA
Sbjct: 121 IAYEDCWTALKWVAAHSAGTGPEEWLNEIADLNRVYFAGDSAGANIANKMAIRVGIEGVA 180

Query: 181 GLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPN 240
           GLNLKGLML+HPYFWGEKLIGDEEKLKPE+RWFIEKLWY+ACPTISGLDDP+VNPEFEP 
Sbjct: 181 GLNLKGLMLIHPYFWGEKLIGDEEKLKPEDRWFIEKLWYLACPTISGLDDPVVNPEFEPK 240

Query: 241 LGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPTSDD 300
           LG + AERVAVYVAEKD+LKDRG FYSECLKKSGWGGAVEV+ETKGQGHVFHLFNPTSDD
Sbjct: 241 LGGLAAERVAVYVAEKDSLKDRGWFYSECLKKSGWGGAVEVVETKGQGHVFHLFNPTSDD 300

Query: 301 TVQFVGKLAAFLNGG 316
            V FVGKLAAF+NGG
Sbjct: 301 AVDFVGKLAAFINGG 315

BLAST of CSPI02G11580 vs. NCBI nr
Match: XP_038902770.1 (probable carboxylesterase 13 [Benincasa hispida])

HSP 1 Score: 556.2 bits (1432), Expect = 1.8e-154
Identity = 259/314 (82.48%), Postives = 285/314 (90.76%), Query Frame = 0

Query: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60
           MASTEIA++ SPML+VYKDGR  RL GNEL+ PSLDPLT VESKD+VISPETPVSARIY+
Sbjct: 1   MASTEIAHDFSPMLVVYKDGRIHRLAGNELIDPSLDPLTGVESKDVVISPETPVSARIYK 60

Query: 61  PKPTAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLP 120
           PKPTA+PHKLPLL+Y HGGGFCIESAFS  YH HLNSLVAEANV+A+SV+YR APEHPLP
Sbjct: 61  PKPTAQPHKLPLLVYFHGGGFCIESAFSSFYHRHLNSLVAEANVVAVSVDYRLAPEHPLP 120

Query: 121 VAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA 180
           +AYEDSW ALKWVAAHSAGTGPEEWLN IAD +RVYFAGDSAGAN+AN+MAIRVG EG+A
Sbjct: 121 IAYEDSWDALKWVAAHSAGTGPEEWLNGIADLDRVYFAGDSAGANIANRMAIRVGTEGLA 180

Query: 181 GLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPN 240
           GLNL+GL+LVH YFWG KLIGDEEKL PE R FIEKLWY  CP ISGLDDPIVNP F+P 
Sbjct: 181 GLNLRGLLLVHAYFWGGKLIGDEEKLNPEHRSFIEKLWYFVCPKISGLDDPIVNPGFDPE 240

Query: 241 LGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPTSDD 300
           LG+V AERVAV+VAEKDALK+RG FYSECLKKSGWGGAV+V+ETKGQGHVFHLFNPTSD+
Sbjct: 241 LGRVAAERVAVFVAEKDALKNRGWFYSECLKKSGWGGAVDVIETKGQGHVFHLFNPTSDE 300

Query: 301 TVQFVGKLAAFLNG 315
            V FVGKLAAF+NG
Sbjct: 301 AVDFVGKLAAFING 314

BLAST of CSPI02G11580 vs. NCBI nr
Match: XP_038900533.1 (probable carboxylesterase 2 [Benincasa hispida])

HSP 1 Score: 540.8 bits (1392), Expect = 7.8e-150
Identity = 253/315 (80.32%), Postives = 280/315 (88.89%), Query Frame = 0

Query: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60
           MASTEIA++ SP+L+VYKDGR  RL GNELV PSLDPLT VESKD+VISPET VS RIYR
Sbjct: 1   MASTEIAHDFSPLLVVYKDGRVHRLAGNELVDPSLDPLTDVESKDVVISPETAVSVRIYR 60

Query: 61  PKPTAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLP 120
           PKPTA+PHKLPLLIY HGG FCIESAFS TY+ HLNSLVAEANVIA+SV+YR APEHPLP
Sbjct: 61  PKPTAQPHKLPLLIYFHGGCFCIESAFSTTYNRHLNSLVAEANVIAVSVDYRLAPEHPLP 120

Query: 121 VAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA 180
           +AYEDSW ALKWVAAHSAGTGPEEWLN IAD  RVYFAGDSAGAN+AN+MAIRVG EG +
Sbjct: 121 IAYEDSWDALKWVAAHSAGTGPEEWLNGIADLERVYFAGDSAGANIANRMAIRVGSEGSS 180

Query: 181 GLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEFEPN 240
           GLNL+GL+LVHPYFWG KLIGDEEKL P++R FIEKLWY   P ISGLDDPIVNPEF+P 
Sbjct: 181 GLNLRGLLLVHPYFWGGKLIGDEEKLNPKDRMFIEKLWYFVYPKISGLDDPIVNPEFDPE 240

Query: 241 LGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPTSDD 300
           LG+V AERVAVYVAEKDA+K+RG  YSECL+KSGW G V+V+ETKGQGHVFHLFNPTSD+
Sbjct: 241 LGRVAAERVAVYVAEKDAVKNRGWLYSECLQKSGWSGTVDVVETKGQGHVFHLFNPTSDE 300

Query: 301 TVQFVGKLAAFLNGG 316
            V F+GKLAAF+N G
Sbjct: 301 AVDFIGKLAAFINAG 315

BLAST of CSPI02G11580 vs. TAIR 10
Match: AT3G48700.1 (carboxyesterase 13 )

HSP 1 Score: 307.4 bits (786), Expect = 1.4e-83
Identity = 160/325 (49.23%), Postives = 217/325 (66.77%), Query Frame = 0

Query: 4   TEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYRPK- 63
           +EIA + SPMLI+YK GR ERLVG   V PS +P   V SKD+V SP+  +S RIY P+ 
Sbjct: 3   SEIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEK 62

Query: 64  -PTAEPH---KLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHP 123
             TAE     KLPLL+Y HGGGF +E+AFSPTYH  L + V+ ++ +A+SV+YRRAPEHP
Sbjct: 63  AATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHP 122

Query: 124 LPVAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEG 183
           +P +Y+DSWTALKWV +H AG+G E+WLNK ADF++V+ AGDSAGAN+ + M ++   + 
Sbjct: 123 IPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDK 182

Query: 184 VA--GLN---LKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACP-TISGLDDPI 243
           ++   LN   + G++LVHPYFW +  + D+E      R +IE +W +A P +  G DDP 
Sbjct: 183 LSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPF 242

Query: 244 VN--PEFEPNLGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAV-EVMETKGQGH 303
           +N       +L  +   +V V VAEKDAL  +G  Y E L KS W G V +V+ETKG+GH
Sbjct: 243 INVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGH 302

Query: 304 VFHLFNPTSDDTVQFVGKLAAFLNG 315
           VFHL +P S+   + V + A F+ G
Sbjct: 303 VFHLRDPNSEKAHELVHRFAGFIKG 327

BLAST of CSPI02G11580 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 305.8 bits (782), Expect = 4.0e-83
Identity = 154/321 (47.98%), Postives = 213/321 (66.36%), Query Frame = 0

Query: 4   TEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYRPKP 63
           +EIA + SP+L +YK GR ERL+G   V PS +P   V SKD+V S +  +S RIY P+ 
Sbjct: 3   SEIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEK 62

Query: 64  TA--EPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLPV 123
            A     KLPLL+Y HGGGF IE+AFSPTYH  L + V+ +N +A+SV+YRRAPEHP+ V
Sbjct: 63  AAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPISV 122

Query: 124 AYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA- 183
            ++DSWTALKWV  H  G+G E+WLNK ADF+RV+ +GDSAGAN+ + MA+R   E ++ 
Sbjct: 123 PFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSP 182

Query: 184 GLN---LKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACP-TISGLDDPIVN-- 243
           GLN   + G++L+HPYFW +  I +++      R  IE  W +A P +  G DDP++N  
Sbjct: 183 GLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVV 242

Query: 244 PEFEPNLGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLF 303
                +L  +   +V V VAEKDAL  +G  Y+  L+KSGW G VEV+E++G+ HVFHL 
Sbjct: 243 QSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLL 302

Query: 304 NPTSDDTVQFVGKLAAFLNGG 316
            P  D+ ++ + K + F+ GG
Sbjct: 303 KPECDNAIEVMHKFSGFIKGG 323

BLAST of CSPI02G11580 vs. TAIR 10
Match: AT2G03550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 301.6 bits (771), Expect = 7.4e-82
Identity = 158/309 (51.13%), Postives = 196/309 (63.43%), Query Frame = 0

Query: 6   IAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYRPKPTA 65
           IA++ SPM  VYK GR ERL+G   V PSL P   V SKDI+ SPE  +S RIY P+   
Sbjct: 5   IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKVT 64

Query: 66  EPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLPVAYED 125
              KLP+LIY HGGGF IE+AFSP YH  L S VA AN +AISV YRRAPE P+P+ YED
Sbjct: 65  -VKKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPIPYED 124

Query: 126 SWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVAGLNLK 185
           SW +LKWV  H  GTGPE W+NK  DF +V+ AGDSAG N+++ + +R   E +    + 
Sbjct: 125 SWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCDSLIS 184

Query: 186 GLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACP-TISGLDDPIVNPEFEPNLGKV 245
           G++L+HPYFW +  I + E     +   +E  W VA P +  G+DDP +N       G +
Sbjct: 185 GIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDPSG-L 244

Query: 246 TAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPTSDDTVQF 305
              RV V VA  D    +G  Y+E LKKSGW G VEVMETK +GHVFHL NP SD+  Q 
Sbjct: 245 GCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNARQV 304

Query: 306 VGKLAAFLN 314
           V KL  F+N
Sbjct: 305 VKKLEEFIN 311

BLAST of CSPI02G11580 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 301.2 bits (770), Expect = 9.7e-82
Identity = 151/312 (48.40%), Postives = 202/312 (64.74%), Query Frame = 0

Query: 5   EIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYRPKPT 64
           +++  L P L+V+ DG  ERL G E+  P LDP+T V SKDI+I P+T +SARIYRP   
Sbjct: 7   QVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIYRPFSI 66

Query: 65  AEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLPVAYE 124
               K+PL++Y HGG F I S   P+YH  LN +V +ANVIA+SV YR APEHPLP AYE
Sbjct: 67  QPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLPTAYE 126

Query: 125 DSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVAGLNL 184
           DSWTALK + A +     E W+N  AD + ++  GDSAGAN+++ +A R   +    L +
Sbjct: 127 DSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFR-AKQSDQTLKI 186

Query: 185 KGLMLVHPYFWGEKLIGDEEKLKPEER-WFIEKLWYVACPTISGLDDPIVNP--EFEPNL 244
           KG+ ++HPYFWG + IG E  +K E R   ++  W   CP+  G DDP +NP  +  P+L
Sbjct: 187 KGIGMIHPYFWGTQPIGAE--IKDEARKQMVDGWWEFVCPSEKGSDDPWINPFADGSPDL 246

Query: 245 GKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPTSDDT 304
           G +  ERV + VAEKD L +RG+ Y E L KS W G VE+METK + HVFH+F P  D+ 
Sbjct: 247 GGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDCDEA 306

Query: 305 VQFVGKLAAFLN 314
           ++ V  LA F+N
Sbjct: 307 MEMVRCLALFIN 310

BLAST of CSPI02G11580 vs. TAIR 10
Match: AT1G49660.1 (carboxyesterase 5 )

HSP 1 Score: 288.9 bits (738), Expect = 5.0e-78
Identity = 141/317 (44.48%), Postives = 203/317 (64.04%), Query Frame = 0

Query: 4   TEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYRPKP 63
           +EIA    P   +YKDGR ERL+G + +  SLDP   V SKD++ SPE  +S R++ P  
Sbjct: 3   SEIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHK 62

Query: 64  TAE---PHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEHPLP 123
           + +    +KLPLLIYIHGG + IES FSP YH++L  +V  AN +A+SV+YRRAPE P+P
Sbjct: 63  STKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVP 122

Query: 124 VAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGMEGVA 183
            AYED W+A++W+ AHS G+GP +W+NK ADF +V+  GDSAG N+++ MA++ G E   
Sbjct: 123 AAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKL 182

Query: 184 GLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLW-YVACP-TISGLDDPIVNPEFE 243
            L +KG+ +VHP FWG   + + +    E R  I ++W  +A P +++G DDP+ N    
Sbjct: 183 DLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGS 242

Query: 244 -PNLGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVMETKGQGHVFHLFNPT 303
             +   +  ++V V VA KD    +G  Y+  L+K  W G VEV+E +G+ HVFHL NP 
Sbjct: 243 GSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPK 302

Query: 304 SDDTVQFVGKLAAFLNG 315
           SD  ++F+ K   F+ G
Sbjct: 303 SDKALKFLKKFVEFIIG 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SMM91.9e-8249.23Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1[more]
Q9SMN05.6e-8247.98Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1[more]
Q9ZQ911.0e-8051.13Probable carboxylesterase 7 OS=Arabidopsis thaliana OX=3702 GN=CXE7 PE=2 SV=1[more]
Q9SX781.4e-8048.40Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1[more]
Q9FX947.0e-7744.48Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LIH81.8e-18499.05Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G2345... [more]
A0A5A7SPY32.5e-17090.16Putative carboxylesterase 13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3CAV32.5e-17090.16probable carboxylesterase 13 OS=Cucumis melo OX=3656 GN=LOC103498400 PE=4 SV=1[more]
A0A6J1FW981.0e-14275.24probable carboxylesterase 13 OS=Cucurbita moschata OX=3662 GN=LOC111449075 PE=4 ... [more]
A0A6J1JBI91.9e-14174.45probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111484271 PE=4 SV... [more]
Match NameE-valueIdentityDescription
KAE8651854.13.7e-18499.05hypothetical protein Csa_006663 [Cucumis sativus][more]
XP_004154254.13.7e-18499.05probable carboxylesterase 13 [Cucumis sativus][more]
XP_008459209.25.2e-17090.16PREDICTED: probable carboxylesterase 13 [Cucumis melo] >KAA0032066.1 putative ca... [more]
XP_038902770.11.8e-15482.48probable carboxylesterase 13 [Benincasa hispida][more]
XP_038900533.17.8e-15080.32probable carboxylesterase 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G48700.11.4e-8349.23carboxyesterase 13 [more]
AT3G48690.14.0e-8347.98alpha/beta-Hydrolases superfamily protein [more]
AT2G03550.17.4e-8251.13alpha/beta-Hydrolases superfamily protein [more]
AT1G47480.19.7e-8248.40alpha/beta-Hydrolases superfamily protein [more]
AT1G49660.15.0e-7844.48carboxyesterase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 73..292
e-value: 1.2E-47
score: 162.5
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 8..314
e-value: 1.1E-67
score: 230.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 11..313
NoneNo IPR availablePANTHERPTHR23024:SF458CARBOXYLESTERASE 2-RELATEDcoord: 3..313
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 3..313

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G11580.1CSPI02G11580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity