CSPI02G08280 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G08280
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	sphinganine C4-monooxygenase 1-like
Location	Chr2: 7774089 .. 7776698 (-)
RNA-Seq Expression	CSPI02G08280
Synteny	CSPI02G08280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGTAAAAGAAAAAATCTCTCTTCGTGTAAACCAGAGTGGGAAGGTTGTTCCCGATTTCCTTCCCAACGCCTAGCAGATTTCTGATTTGGGGATTTATGGTTCTCTCTTCCAATCTCTAAAATCTCTACCTCTTTCCCTTTTCAATGGAATTCACCATCTCCGATGATTTGTTGGGTACTTTCGTCCCTATTTTCGTTTATTGGTTCTATTCTGGCTTATACCTTCTTCTTGGTTTCTTTGACAATTATCGATTGCACTCCAAGACAGATGAGGATGAGAAGAACTTGGTTTCTAAATCAACTGTTGTTCGGGGCGTTCTTTTTCAGCAATTCATTCAGGCTATTGTCGCCATCATCCTCTTTAAGGTGACCTTCTTTTTTTTTTTTTTTTTTCTTTTCTTTTCTTTTACCTATTTTTTCAATTCTTAATCGTTGCTCTTTGTTAATTTTGTTGTTATTAAGCTCATTTCTGATTCTAATTGTTTGTGAAACTTACCCTAAAATATTTCAACTGAGATTGTATTTTGCTCTAGACTTTGCCGAAACTCTTTGTTTGATCTTCCTGAATTTTTCTTGGTTTATCACCTCTTTGTGGTACAGTTTGATTATAGTTTTACTAGACTACATACAAATTCGTTGTGGGTTTCCTTGTTTCTGGACCTATTCAGTTTGACAAAAATCTAAATAATTACTTCTCTTCTGTTTACATGTGTTTTAAATTTTGATTTTGACAAGAACAGACTAGACACTGGATGGGGGGAGTGAAGAAGCAATGTCATTGTTGACCTATTATCAACTTTCGGTTTCTTATAATTACAACTATTGTATATCTTAAACAGGATTTGTTGCTTACTTTTGTTACTTTAGTTCGAATTGATGGAGCAAAATTTTAAACCTTTTCACATCAAAATAATTTAGATGGCAATTGAATTTTTTTTAATTTGGTTCAAGAATAAGAGGAGTTAATGATTCTAAGTTAAGGAAGTTGAAGGACAAAATCTGGTTATTAGCTTTTGACTGTAAGTATGTTTTGTTTCGAACACTACAAGGAAGGCTTTTGGTGAATACTTTTAACTTCAAGATAGACGTGAACAAAATTTTTGCATATTACCCCTTTTTCATAGCTAAATATTCTCTATGAGATGATTTTTTGGACTAGAAAGTTATGGGCTAAAACATGATATTTTTGTTTTCTTTGTAAATTAAATTCCATTTCCTTTAAGTATATTGACCTTTTGATTTGTCTATTCGTTTTAGGTTACATGTTTATTCACAAGTTTTAGGACCAATCCCCATTTGTGTTCTGCATCGGCACTCAGTTGGAAGTCACCAACTTGCAAACAAAAAAATCTTGAACTTCTGTTTAATTTTGTGGTGTTTTGCTTGTATTTCCTATAATGTTGTTGTCATTGCTTCTAATCCGTGAAGTTCCTTTTCTTTTTGAGTCCCATGCTAAACTAGCTTAGCAGTTGTTCGTGTGAAATATTGTAGACTGAACTAAAACTATTGTTATTATTAACCTCATCCAAGTGCTGTTAGATGAATTGTTCGGTTTGTCGTCCCTGTGGTTAAAGTGGCAAGTCCTGAACTCAAATGTTTTGAGCATACGAGCTCAAACTAAGATGCCACCTATTTGGGGCATCTTAGAAATCCTGGTTTTGTAATATTCTAAAGTTTAGTAGTTGTCTCGTGAGATTATTCAAAATGTATGTAAACTAACTCAGAGACTTAGGTTTTTGAAATAGAGAAAAGAAAAGGAATCAATGAGAACTTTGGCATAAGTCATTGATATTATGTGGGGTGTCTCTGCACTTACGTTCGAGTTTGGTCTCTGAATTTATAATTGCATTTTGTTATGTTCAGTTGACTGGAAATGATGATGGAAGCAATGCAGTTCCAAAGTCATGGCCAATGGTTGTTTTGCAATTTTTAACTGCGATGTTCATTCTAGACACGTGGCAGTACTTTGCACACCGATACATGCATCATAACAAGTTTCTGTACAGACACATTCATTCTCAGCACCATAGGCTTGTTGTTCCATATGCATTTGGGGCTTTGTATAATCATCCAGTGGAAGGTCTACTGCTTGACACGATTGGTGGGGCGTTGTCATTTTTAGCATCTGGTATGACTCCTAGAATCTCCATCTTCTTCTTTTCCTTTGCCACTATTAAGACGGTAGACGATCACTGTGGATTATGGCTGCCTGGTAATCTCTTCCACATCTTATTTCGCAACAATACTGCATATCACGACGTCCACCATCAACTATACGGCAGCAAGTTTAATTTTTCACAGCCTTTCTTTGTTACATGGGATAGAATACTTGGTACCTATATGCCTTACTCCTTGGAGAAGAGAGCAAGCGGCGGCTTTGAAGCACGACCAAAGATTGAATAAATACCGTAAGCAGCAATCAGTTGCTTATATTATATTATTTGTATCCCACAACTATATGATTTGTATATTACTCCTATTGTTGCCATTTTTATACTTTTTCCCCATTTCCCCTCTCTTCCCTAACATAGATTTGAAACATGAGTTCAGATGAGCTAGGTTGTGTTTTGATATATATATATTTTTTAAAAAAAATTTATAATTTTTTAG

mRNA sequence

GGGTAAAAGAAAAAATCTCTCTTCGTGTAAACCAGAGTGGGAAGGTTGTTCCCGATTTCCTTCCCAACGCCTAGCAGATTTCTGATTTGGGGATTTATGGTTCTCTCTTCCAATCTCTAAAATCTCTACCTCTTTCCCTTTTCAATGGAATTCACCATCTCCGATGATTTGTTGGGTACTTTCGTCCCTATTTTCGTTTATTGGTTCTATTCTGGCTTATACCTTCTTCTTGGTTTCTTTGACAATTATCGATTGCACTCCAAGACAGATGAGGATGAGAAGAACTTGGTTTCTAAATCAACTGTTGTTCGGGGCGTTCTTTTTCAGCAATTCATTCAGGCTATTGTCGCCATCATCCTCTTTAAGTTGACTGGAAATGATGATGGAAGCAATGCAGTTCCAAAGTCATGGCCAATGGTTGTTTTGCAATTTTTAACTGCGATGTTCATTCTAGACACGTGGCAGTACTTTGCACACCGATACATGCATCATAACAAGTTTCTGTACAGACACATTCATTCTCAGCACCATAGGCTTGTTGTTCCATATGCATTTGGGGCTTTGTATAATCATCCAGTGGAAGGTCTACTGCTTGACACGATTGGTGGGGCGTTGTCATTTTTAGCATCTGGTATGACTCCTAGAATCTCCATCTTCTTCTTTTCCTTTGCCACTATTAAGACGGTAGACGATCACTGTGGATTATGGCTGCCTGGTAATCTCTTCCACATCTTATTTCGCAACAATACTGCATATCACGACGTCCACCATCAACTATACGGCAGCAAGTTTAATTTTTCACAGCCTTTCTTTGTTACATGGGATAGAATACTTGGTACCTATATGCCTTACTCCTTGGAGAAGAGAGCAAGCGGCGGCTTTGAAGCACGACCAAAGATTGAATAAATACCGTAAGCAGCAATCAGTTGCTTATATTATATTATTTGTATCCCACAACTATATGATTTGTATATTACTCCTATTGTTGCCATTTTTATACTTTTTCCCCATTTCCCCTCTCTTCCCTAACATAGATTTGAAACATGAGTTCAGATGAGCTAGGTTGTGTTTTGATATATATATATTTTTTAAAAAAAATTTATAATTTTTTAG

Coding sequence (CDS)

ATGGAATTCACCATCTCCGATGATTTGTTGGGTACTTTCGTCCCTATTTTCGTTTATTGGTTCTATTCTGGCTTATACCTTCTTCTTGGTTTCTTTGACAATTATCGATTGCACTCCAAGACAGATGAGGATGAGAAGAACTTGGTTTCTAAATCAACTGTTGTTCGGGGCGTTCTTTTTCAGCAATTCATTCAGGCTATTGTCGCCATCATCCTCTTTAAGTTGACTGGAAATGATGATGGAAGCAATGCAGTTCCAAAGTCATGGCCAATGGTTGTTTTGCAATTTTTAACTGCGATGTTCATTCTAGACACGTGGCAGTACTTTGCACACCGATACATGCATCATAACAAGTTTCTGTACAGACACATTCATTCTCAGCACCATAGGCTTGTTGTTCCATATGCATTTGGGGCTTTGTATAATCATCCAGTGGAAGGTCTACTGCTTGACACGATTGGTGGGGCGTTGTCATTTTTAGCATCTGGTATGACTCCTAGAATCTCCATCTTCTTCTTTTCCTTTGCCACTATTAAGACGGTAGACGATCACTGTGGATTATGGCTGCCTGGTAATCTCTTCCACATCTTATTTCGCAACAATACTGCATATCACGACGTCCACCATCAACTATACGGCAGCAAGTTTAATTTTTCACAGCCTTTCTTTGTTACATGGGATAGAATACTTGGTACCTATATGCCTTACTCCTTGGAGAAGAGAGCAAGCGGCGGCTTTGAAGCACGACCAAAGATTGAATAA

Protein sequence

MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLFQQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFLYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKTVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEKRASGGFEARPKIE*

Homology

[Close]

BLAST of CSPI02G08280 vs. ExPASy Swiss-Prot
Match: Q8VYI1 (Sphinganine C4-monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=SBH1 PE=1 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 5.4e-112
Identity = 188/254 (74.02%), Postives = 220/254 (86.61%), Query Frame = 0


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M F +SD+LLGT  PI VYW YSG+Y+ L   ++YRLHSK +E+EKNLVSKS+VV+GVL 
Sbjct: 3   MGFAVSDELLGTVAPIVVYWLYSGIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLV 62

Query: 61  QQFIQAIVAIILFKLTGND-DGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKF 120
           QQ +QA+VAI+LF +TG+D +   A   S+ ++  QF+TAM +LDTWQYF HRYMH NKF
Sbjct: 63  QQVVQAVVAILLFTVTGSDAEADKAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKF 122

Query: 121 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIK 180
           LY+HIHSQHHRL+VPYA+GALYNHPVEGLLLDTIGGALSFL SGM+PR SIFFFSFATIK
Sbjct: 123 LYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIK 182

Query: 181 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGNLFH++F+NN+AYHD+HHQLYG+K+NFSQPFFV WDRILGTYMPYSLE
Sbjct: 183 TVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWDRILGTYMPYSLE 242

Query: 241 KRASGGFEARPKIE 254
           KR  GGFEARP  E
Sbjct: 243 KREDGGFEARPTKE 256

[Close]

BLAST of CSPI02G08280 vs. ExPASy Swiss-Prot
Match: Q9AST3 (Sphinganine C4-monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=SBH2 PE=1 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 1.2e-111
Identity = 188/251 (74.90%), Postives = 216/251 (86.06%), Query Frame = 0


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M F ISD+ LGTFVPI VYW YSG+Y+ LG  D YRLHSK DEDEKNLVSKS VV+GVL 
Sbjct: 2   MSFVISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLL 61

Query: 61  QQFIQAIVAIILFKLTGND-DGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKF 120
           QQ +QAI+++ILFK+TG+D D +     S  ++  QF+ AM ++DTWQYF HRYMH NKF
Sbjct: 62  QQTLQAIISVILFKITGSDADAATTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNKF 121

Query: 121 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIK 180
           LY+HIHSQHHRL+VPY++GALYNHP+EGLLLDTIGGALSFL SGM+PR +IFFFSFATIK
Sbjct: 122 LYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFSFATIK 181

Query: 181 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGN FHI F NN+AYHDVHHQLYG+K+NFSQPFFV WDRILGTY+PYSLE
Sbjct: 182 TVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWDRILGTYLPYSLE 241

Query: 241 KRASGGFEARP 251
           KRA+GGFE RP
Sbjct: 242 KRANGGFETRP 252

[Close]

BLAST of CSPI02G08280 vs. ExPASy Swiss-Prot
Match: B8B4W4 (Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. indica OX=39946 GN=GL1-9 PE=3 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 1.6e-95
Identity = 166/251 (66.14%), Postives = 202/251 (80.48%), Query Frame = 0


Query: 5   ISDDLLGTFVPIFVYWFYSGLYLLLGF---FDNYRLHSKTDEDEKNLVSKSTVVRGVLFQ 64
           +SD+ +GTF PI +YW Y+G Y L+      + YRLH++ +E+EKNLV+   VVRGVL Q
Sbjct: 8   VSDETMGTFAPIALYWVYAGGYQLVLHRRPLERYRLHTRAEEEEKNLVALPAVVRGVLLQ 67

Query: 65  QFIQAIVAIILFKLTGNDDGSNAVPKSWPMVV--LQFLTAMFILDTWQYFAHRYMHHNKF 124
           Q +QAIVA+ILF +T +   S+AV    P+++   QFL AM ++D+WQYF HRYMH NKF
Sbjct: 68  QLVQAIVAMILFMVTSD---SSAVVVQPPIIIQAFQFLVAMLVMDSWQYFVHRYMHQNKF 127

Query: 125 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIK 184
           LYRHIHSQHHRL+VPYA GALYNHP+EGLLLDT+GGA+SFL SGMTPR S+FFF FA +K
Sbjct: 128 LYRHIHSQHHRLIVPYAIGALYNHPLEGLLLDTVGGAISFLVSGMTPRTSVFFFCFAVLK 187

Query: 185 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 244
           TVDDHCGLWLP N+F  LF+NNTAYHDVHHQL GSK+N+SQPFF  WDRILGT+MPY+L 
Sbjct: 188 TVDDHCGLWLPYNIFQSLFQNNTAYHDVHHQLQGSKYNYSQPFFSIWDRILGTHMPYNLV 247

Query: 245 KRASGGFEARP 251
           +R  GGFEARP
Sbjct: 248 RRKEGGFEARP 255

[Close]

BLAST of CSPI02G08280 vs. ExPASy Swiss-Prot
Match: Q0D4G3 (Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. japonica OX=39947 GN=GL1-9 PE=2 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 1.6e-95
Identity = 166/251 (66.14%), Postives = 202/251 (80.48%), Query Frame = 0


Query: 5   ISDDLLGTFVPIFVYWFYSGLYLLLGF---FDNYRLHSKTDEDEKNLVSKSTVVRGVLFQ 64
           +SD+ +GTF PI +YW Y+G Y L+      + YRLH++ +E+EKNLV+   VVRGVL Q
Sbjct: 8   VSDETMGTFAPIALYWVYAGGYQLVLHRRPLERYRLHTRAEEEEKNLVALPAVVRGVLLQ 67

Query: 65  QFIQAIVAIILFKLTGNDDGSNAVPKSWPMVV--LQFLTAMFILDTWQYFAHRYMHHNKF 124
           Q +QAIVA+ILF +T +   S+AV    P+++   QFL AM ++D+WQYF HRYMH NKF
Sbjct: 68  QLVQAIVAMILFMVTSD---SSAVVVQPPIIIQAFQFLVAMLVMDSWQYFVHRYMHQNKF 127

Query: 125 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIK 184
           LYRHIHSQHHRL+VPYA GALYNHP+EGLLLDT+GGA+SFL SGMTPR S+FFF FA +K
Sbjct: 128 LYRHIHSQHHRLIVPYAIGALYNHPLEGLLLDTVGGAISFLVSGMTPRTSVFFFCFAVLK 187

Query: 185 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 244
           TVDDHCGLWLP N+F  LF+NNTAYHDVHHQL GSK+N+SQPFF  WDRILGT+MPY+L 
Sbjct: 188 TVDDHCGLWLPYNIFQSLFQNNTAYHDVHHQLQGSKYNYSQPFFSIWDRILGTHMPYNLV 247

Query: 245 KRASGGFEARP 251
           +R  GGFEARP
Sbjct: 248 RRKEGGFEARP 255

[Close]

BLAST of CSPI02G08280 vs. ExPASy Swiss-Prot
Match: O94298 (Sphingolipid C4-hydroxylase sur2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sur2 PE=3 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 4.6e-55
Identity = 109/253 (43.08%), Postives = 149/253 (58.89%), Query Frame = 0


Query: 15  PIFVYWFYSGL-----YLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLFQQFIQAIVA 74
           P+ +YW  S       Y+ L  F+ YR+H   +   +N V +  VV+ VLFQQ  + +V 
Sbjct: 21  PVIIYWVASAFFGFLHYIELPVFEKYRIHPPEEIARRNRVPQMAVVKAVLFQQLCEVVVG 80

Query: 75  IILFKLTGN----DDGSNAV------PKSWPMVV-----------------LQFLTAMFI 134
           I L    G     D+    +       K+ P ++                  Q+  A FI
Sbjct: 81  IALAMFEGYPEPIDEAKQMLRYEAFFSKNLPALLQVAPFAPKLAYNFIVPAFQYFFAFFI 140

Query: 135 LDTWQYFAHRYMHHNKFLYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLAS 194
           +D+WQYF HRY+H+NK LY  IH+ HHRL VPYA GALYNHP EGL+LDT G  +++LA+
Sbjct: 141 IDSWQYFWHRYLHYNKKLYNMIHAHHHRLQVPYAMGALYNHPFEGLILDTFGAGVAYLAA 200

Query: 195 GMTPRISIFFFSFATIKTVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPF 236
           G++P+ ++ FF+ +T+KTVDDHCG   P +   + F NN  YHD+HHQ YG + NFSQPF
Sbjct: 201 GLSPQQAVIFFTLSTLKTVDDHCGYVFPYDPLQMFFANNARYHDLHHQPYGFQKNFSQPF 260

[Close]

BLAST of CSPI02G08280 vs. ExPASy TrEMBL
Match: A0A5D3CZU2 (Sphinganine C4-monooxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1327G00180 PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 1.5e-144
Identity = 247/253 (97.63%), Postives = 252/253 (99.60%), Query Frame = 0


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFK+TGNDDGSNAVPKSWPMVVLQFLTAMF+LDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSNAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDT+GGALSFLASGMTPR+SIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RASGGFEARPKIE 254
           RA GGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CSPI02G08280 vs. ExPASy TrEMBL
Match: A0A1S3B426 (sphinganine C4-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103485785 PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 1.5e-144
Identity = 247/253 (97.63%), Postives = 252/253 (99.60%), Query Frame = 0


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFK+TGNDDGSNAVPKSWPMVVLQFLTAMF+LDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSNAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDT+GGALSFLASGMTPR+SIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RASGGFEARPKIE 254
           RA GGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CSPI02G08280 vs. ExPASy TrEMBL
Match: A0A6J1DY75 (sphinganine C4-monooxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111025684 PE=3 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 2.4e-139
Identity = 237/253 (93.68%), Postives = 249/253 (98.42%), Query Frame = 0


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISD+LLGTFVPIFVYW YSG+Y+LLGFF+NYRLHSK DEDEKNLVSK+TVVRGVLF
Sbjct: 1   MEFTISDELLGTFVPIFVYWLYSGIYVLLGFFENYRLHSKKDEDEKNLVSKATVVRGVLF 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFK+TGNDDGS+AVPKSWPMVVLQFLTAMF+LDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSSAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           YR+IHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFL SGMTPR+SIFFFSFATIKT
Sbjct: 121 YRYIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSK+NFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKYNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RASGGFEARPKIE 254
           RA GGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CSPI02G08280 vs. ExPASy TrEMBL
Match: A0A6J1GBQ9 (sphinganine C4-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452712 PE=3 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 3.5e-138
Identity = 235/253 (92.89%), Postives = 247/253 (97.63%), Query Frame = 0


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M FTISDDLLGTFVPIFVYWFYSG+Y+LLGFF+NYRLHSK DEDEKNLVSKSTVVRGVLF
Sbjct: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVLLGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFK+TGNDDGS AVPKSWP+VVLQFLTAMF+LDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSVAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           +RHIHSQHH+LVVPYAFGALYNHPVEGLLLDT+GGALSFL SGMTPR+SIFFFSFATIKT
Sbjct: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSKFNFSQPFFV WDRILGTY+PYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240

Query: 241 RASGGFEARPKIE 254
           RA GGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CSPI02G08280 vs. ExPASy TrEMBL
Match: A0A6J1KH66 (sphinganine C4-monooxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493180 PE=3 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 5.9e-138
Identity = 234/253 (92.49%), Postives = 247/253 (97.63%), Query Frame = 0


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M FTISDDLLGTFVPIFVYWFYSG+Y++LGFF+NYRLHSK DEDEKNLVSKSTVVRGVLF
Sbjct: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVILGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFK+TGNDDGS AVPKSWP+VVLQFLTAMF+LDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSIAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           +RHIHSQHH+LVVPYAFGALYNHPVEGLLLDT+GGALSFL SGMTPR+SIFFFSFATIKT
Sbjct: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSKFNFSQPFFV WDRILGTY+PYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240

Query: 241 RASGGFEARPKIE 254
           RA GGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CSPI02G08280 vs. NCBI nr
Match: XP_004138918.1 (sphinganine C4-monooxygenase 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 526.2 bits (1354), Expect = 1.6e-145
Identity = 253/253 (100.00%), Postives = 253/253 (100.00%), Query Frame = 0


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RASGGFEARPKIE 254
           RASGGFEARPKIE
Sbjct: 241 RASGGFEARPKIE 253

[Close]

BLAST of CSPI02G08280 vs. NCBI nr
Match: XP_008441706.1 (PREDICTED: sphinganine C4-monooxygenase 1-like [Cucumis melo] >KAA0032268.1 sphinganine C4-monooxygenase 1-like [Cucumis melo var. makuwa] >TYK17072.1 sphinganine C4-monooxygenase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 521.9 bits (1343), Expect = 3.0e-144
Identity = 247/253 (97.63%), Postives = 252/253 (99.60%), Query Frame = 0


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFK+TGNDDGSNAVPKSWPMVVLQFLTAMF+LDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSNAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDT+GGALSFLASGMTPR+SIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RASGGFEARPKIE 254
           RA GGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CSPI02G08280 vs. NCBI nr
Match: XP_038889456.1 (sphinganine C4-monooxygenase 1-like [Benincasa hispida])

HSP 1 Score: 511.9 bits (1317), Expect = 3.1e-141
Identity = 241/253 (95.26%), Postives = 250/253 (98.81%), Query Frame = 0


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISDDLLGTFVPIFVYWFYSG+Y+LLGFF+NYRLHSKTDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGIYVLLGFFENYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQ IQAIVAIILFK+TGNDDGSNAVPKSWPMVVLQFLTAMF+LDTWQYFAHRYMHHNKFL
Sbjct: 61  QQIIQAIVAIILFKVTGNDDGSNAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           Y+HIHSQHHRLVVPYAFGALYNHPVEGLLLDT+GGALSFLASGMTPR+SIFFFSFATIKT
Sbjct: 121 YKHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFHI+F NNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHIIFGNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RASGGFEARPKIE 254
           RA GGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CSPI02G08280 vs. NCBI nr
Match: XP_022159270.1 (sphinganine C4-monooxygenase 1-like [Momordica charantia])

HSP 1 Score: 504.6 bits (1298), Expect = 5.0e-139
Identity = 237/253 (93.68%), Postives = 249/253 (98.42%), Query Frame = 0


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISD+LLGTFVPIFVYW YSG+Y+LLGFF+NYRLHSK DEDEKNLVSK+TVVRGVLF
Sbjct: 1   MEFTISDELLGTFVPIFVYWLYSGIYVLLGFFENYRLHSKKDEDEKNLVSKATVVRGVLF 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFK+TGNDDGS+AVPKSWPMVVLQFLTAMF+LDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSSAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           YR+IHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFL SGMTPR+SIFFFSFATIKT
Sbjct: 121 YRYIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSK+NFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKYNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RASGGFEARPKIE 254
           RA GGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CSPI02G08280 vs. NCBI nr
Match: XP_022949326.1 (sphinganine C4-monooxygenase 1-like [Cucurbita moschata] >KAG6607119.1 Sphinganine C4-monooxygenase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036810.1 Sphinganine C4-monooxygenase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 500.7 bits (1288), Expect = 7.2e-138
Identity = 235/253 (92.89%), Postives = 247/253 (97.63%), Query Frame = 0


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M FTISDDLLGTFVPIFVYWFYSG+Y+LLGFF+NYRLHSK DEDEKNLVSKSTVVRGVLF
Sbjct: 1   MGFTISDDLLGTFVPIFVYWFYSGIYVLLGFFENYRLHSKKDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFK+TGNDDGS AVPKSWP+VVLQFLTAMF+LDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSVAVPKSWPVVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           +RHIHSQHH+LVVPYAFGALYNHPVEGLLLDT+GGALSFL SGMTPR+SIFFFSFATIKT
Sbjct: 121 FRHIHSQHHKLVVPYAFGALYNHPVEGLLLDTVGGALSFLVSGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSKFNFSQPFFV WDRILGTY+PYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVIWDRILGTYLPYSLEK 240

Query: 241 RASGGFEARPKIE 254
           RA GGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of CSPI02G08280 vs. TAIR 10
Match: AT1G69640.1 (sphingoid base hydroxylase 1 )

HSP 1 Score: 405.2 bits (1040), Expect = 3.8e-113
Identity = 188/254 (74.02%), Postives = 220/254 (86.61%), Query Frame = 0


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M F +SD+LLGT  PI VYW YSG+Y+ L   ++YRLHSK +E+EKNLVSKS+VV+GVL 
Sbjct: 3   MGFAVSDELLGTVAPIVVYWLYSGIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLV 62

Query: 61  QQFIQAIVAIILFKLTGND-DGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKF 120
           QQ +QA+VAI+LF +TG+D +   A   S+ ++  QF+TAM +LDTWQYF HRYMH NKF
Sbjct: 63  QQVVQAVVAILLFTVTGSDAEADKAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKF 122

Query: 121 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIK 180
           LY+HIHSQHHRL+VPYA+GALYNHPVEGLLLDTIGGALSFL SGM+PR SIFFFSFATIK
Sbjct: 123 LYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIK 182

Query: 181 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGNLFH++F+NN+AYHD+HHQLYG+K+NFSQPFFV WDRILGTYMPYSLE
Sbjct: 183 TVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWDRILGTYMPYSLE 242

Query: 241 KRASGGFEARPKIE 254
           KR  GGFEARP  E
Sbjct: 243 KREDGGFEARPTKE 256

[Close]

BLAST of CSPI02G08280 vs. TAIR 10
Match: AT1G14290.1 (sphingoid base hydroxylase 2 )

HSP 1 Score: 404.1 bits (1037), Expect = 8.5e-113
Identity = 188/251 (74.90%), Postives = 216/251 (86.06%), Query Frame = 0


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M F ISD+ LGTFVPI VYW YSG+Y+ LG  D YRLHSK DEDEKNLVSKS VV+GVL 
Sbjct: 2   MSFVISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLL 61

Query: 61  QQFIQAIVAIILFKLTGND-DGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKF 120
           QQ +QAI+++ILFK+TG+D D +     S  ++  QF+ AM ++DTWQYF HRYMH NKF
Sbjct: 62  QQTLQAIISVILFKITGSDADAATTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNKF 121

Query: 121 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIK 180
           LY+HIHSQHHRL+VPY++GALYNHP+EGLLLDTIGGALSFL SGM+PR +IFFFSFATIK
Sbjct: 122 LYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFSFATIK 181

Query: 181 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGN FHI F NN+AYHDVHHQLYG+K+NFSQPFFV WDRILGTY+PYSLE
Sbjct: 182 TVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWDRILGTYLPYSLE 241

Query: 241 KRASGGFEARP 251
           KRA+GGFE RP
Sbjct: 242 KRANGGFETRP 252

[Close]

BLAST of CSPI02G08280 vs. TAIR 10
Match: AT2G29390.3 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 78.2 bits (191), Expect = 1.1e-14
Identity = 69/240 (28.75%), Postives = 109/240 (45.42%), Query Frame = 0


Query: 10  LGTFVPIFVYWFYSGL-YLLL---GFFDNYRLHSKTDEDEKNLVSKSTVVRGVLFQQFIQ 69
           +G+F+     +F SGL Y+ L   GF  NY++ +K++  E        + R +L+   + 
Sbjct: 17  IGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARLLLYHCCVN 76

Query: 70  ---AIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFLYR 129
               + +  +F+  G +  S+    SW +V  Q L    I D   Y+ HR + H K+LY+
Sbjct: 77  LPLMMASYPVFRFMGME--SSFPLPSWKVVSAQILFYFIIEDFVFYWGHRIL-HTKWLYK 136

Query: 130 HIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMT-PRISI--FFFSFATIK 189
           ++HS HH    P+   + Y HP E L L    G  + +   +T P +     +     I+
Sbjct: 137 NVHSVHHEYATPFGLTSEYAHPAEILFL----GFATIVGPALTGPHLITLWLWMMLRVIE 196

Query: 190 TVDDHCGL---WLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPY 237
           TV+ HCG    W P N   +       +HD HH+L  +K       FV  D I GT   Y
Sbjct: 197 TVEAHCGYHFPWSPSNFLPLY--GGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGY 244

[Close]

BLAST of CSPI02G08280 vs. TAIR 10
Match: AT2G29390.2 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 78.2 bits (191), Expect = 1.1e-14
Identity = 69/240 (28.75%), Postives = 109/240 (45.42%), Query Frame = 0


Query: 10  LGTFVPIFVYWFYSGL-YLLL---GFFDNYRLHSKTDEDEKNLVSKSTVVRGVLFQQFIQ 69
           +G+F+     +F SGL Y+ L   GF  NY++ +K++  E        + R +L+   + 
Sbjct: 24  IGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARLLLYHCCVN 83

Query: 70  ---AIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFLYR 129
               + +  +F+  G +  S+    SW +V  Q L    I D   Y+ HR + H K+LY+
Sbjct: 84  LPLMMASYPVFRFMGME--SSFPLPSWKVVSAQILFYFIIEDFVFYWGHRIL-HTKWLYK 143

Query: 130 HIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMT-PRISI--FFFSFATIK 189
           ++HS HH    P+   + Y HP E L L    G  + +   +T P +     +     I+
Sbjct: 144 NVHSVHHEYATPFGLTSEYAHPAEILFL----GFATIVGPALTGPHLITLWLWMMLRVIE 203

Query: 190 TVDDHCGL---WLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPY 237
           TV+ HCG    W P N   +       +HD HH+L  +K       FV  D I GT   Y
Sbjct: 204 TVEAHCGYHFPWSPSNFLPLY--GGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGY 251

[Close]

BLAST of CSPI02G08280 vs. TAIR 10
Match: AT2G29390.1 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 76.6 bits (187), Expect = 3.1e-14
Identity = 70/250 (28.00%), Postives = 111/250 (44.40%), Query Frame = 0


Query: 10  LGTFVPIFVYWFYSGL-YLLL---GFFDNYRLHSKTDEDEKNLVSKSTVVRGVLFQQFIQ 69
           +G+F+     +F SGL Y+ L   GF  NY++ +K++  E        + R +L+   + 
Sbjct: 24  IGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPE---AQGKCIARLLLYHCCVN 83

Query: 70  ---AIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFLYR 129
               + +  +F+  G +  S+    SW +V  Q L    I D   Y+ HR + H K+LY+
Sbjct: 84  LPLMMASYPVFRFMGME--SSFPLPSWKVVSAQILFYFIIEDFVFYWGHRIL-HTKWLYK 143

Query: 130 HIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMT-PRISI--FFFSFATIK 189
           ++HS HH    P+   + Y HP E L L    G  + +   +T P +     +     I+
Sbjct: 144 NVHSVHHEYATPFGLTSEYAHPAEILFL----GFATIVGPALTGPHLITLWLWMMLRVIE 203

Query: 190 TVDDHCGL---WLPGNLFHIL----------FRNNTAYHDVHHQLYGSKFNFSQPFFVTW 237
           TV+ HCG    W P N   +           F  +  +HD HH+L  +K       FV  
Sbjct: 204 TVEAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLLYTKSGNYSSTFVYM 263

The following BLAST results are available for this feature:

BLAST of CSPI02G08280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYI1	5.4e-112	74.02	Sphinganine C4-monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=SBH1 PE=1 SV=1	[more]
Q9AST3	1.2e-111	74.90	Sphinganine C4-monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=SBH2 PE=1 SV=1	[more]
B8B4W4	1.6e-95	66.14	Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. indica OX=39...	[more]
Q0D4G3	1.6e-95	66.14	Very-long-chain aldehyde decarbonylase GL1-9 OS=Oryza sativa subsp. japonica OX=...	[more]
O94298	4.6e-55	43.08	Sphingolipid C4-hydroxylase sur2 OS=Schizosaccharomyces pombe (strain 972 / ATCC...	[more]

BLAST of CSPI02G08280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CZU2	1.5e-144	97.63	Sphinganine C4-monooxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A1S3B426	1.5e-144	97.63	sphinganine C4-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103485785 PE=3...	[more]
A0A6J1DY75	2.4e-139	93.68	sphinganine C4-monooxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC1110256...	[more]
A0A6J1GBQ9	3.5e-138	92.89	sphinganine C4-monooxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11145271...	[more]
A0A6J1KH66	5.9e-138	92.49	sphinganine C4-monooxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493180 ...	[more]

BLAST of CSPI02G08280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138918.1	1.6e-145	100.00	sphinganine C4-monooxygenase 1 isoform X1 [Cucumis sativus]	[more]
XP_008441706.1	3.0e-144	97.63	PREDICTED: sphinganine C4-monooxygenase 1-like [Cucumis melo] >KAA0032268.1 sphi...	[more]
XP_038889456.1	3.1e-141	95.26	sphinganine C4-monooxygenase 1-like [Benincasa hispida]	[more]
XP_022159270.1	5.0e-139	93.68	sphinganine C4-monooxygenase 1-like [Momordica charantia]	[more]
XP_022949326.1	7.2e-138	92.89	sphinganine C4-monooxygenase 1-like [Cucurbita moschata] >KAG6607119.1 Sphingani...	[more]

BLAST of CSPI02G08280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G69640.1	3.8e-113	74.02	sphingoid base hydroxylase 1	[more]
AT1G14290.1	8.5e-113	74.90	sphingoid base hydroxylase 2	[more]
AT2G29390.3	1.1e-14	28.75	sterol 4-alpha-methyl-oxidase 2-2	[more]
AT2G29390.2	1.1e-14	28.75	sterol 4-alpha-methyl-oxidase 2-2	[more]
AT2G29390.1	3.1e-14	28.00	sterol 4-alpha-methyl-oxidase 2-2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 97..232 e-value: 3.5E-23 score: 82.4
None	No IPR available	PANTHER	PTHR11863	STEROL DESATURASE	coord: 2..249
None	No IPR available	PANTHER	PTHR11863:SF122	SPHINGANINE C4-MONOOXYGENASE 1-LIKE	coord: 2..249

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G08280.1	CSPI02G08280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016491	oxidoreductase activity