CSPI02G01360 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G01360
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	tRNA (guanine(37)-N1)-methyltransferase
Location	Chr2: 920748 .. 925108 (+)
RNA-Seq Expression	CSPI02G01360
Synteny	CSPI02G01360

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGACAGTTCCCGCTGTTAGTCTTCTACTACGGTGAAAATCTCGATTCTGATTCTGATTCTTTCTTTTCAGTGGAAAAATCTCCGAACCCATTACTCTACATTGTTCTATTCTGATTTGAATTTGTTTGCATTGAGTTGGGTGTAATTTTTGTTGTGTTATTGAGTATTGAGAAAAATCGAACTTGTGAATCTATCAATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCCTTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACGCTATGTTTTGTGTTATATTGTTTCGATTTCAATTAATCATTTGGGTAATGGCATTTAGCATTAGTGTTACTCATATACTGTTGTCGTTGAACTGTTCATCTTTCTTATCCGAGTTCATGTTTTTGAAAACTTATCAGAATTCTCCTGATATTTTTTAGTTTTTTTCTTTGAAAAGTATTCTTGTTATTAACTTTTGTTTAAAAGCAGACTTTTCTTTGGCCCTCAGTTCGTTTCTGTTGAAAAATTTTCAGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGGTAAAGATCTCCATCTCCGTCCTCATATTTTTTCTGTTAACTATAAGCTTGGATTGCAATTTCATGTTCTTTATGGAAACTCTTCTGTAGCACATTCTGTTCGCTGAAGTTCCAATTCTTGTTCATTGATTTTCTTCATGTTACCTTAGTGGCTTTCTAGCTCTGTTCATGCATTCAAGTTCTTAAATAGTTCTAAAATTATCGAGCGAGTAATTTTTTATCTTAAAACTTCAATTTCTGTAGGGTATAACCATCAACTTGAATGCATCTTTAGTTAGAAAAGTGTCTAAAAACAGCCTATATATCACATGAAACCAAATTAACGTCGTGTAACTAGGTAATTTTCATAAAAATAATCTCGTTTCCCTTTAAAAATCAATCATTTTTTCTCTTCCTTCTTTTTACCTTATTTTGTCAATGGAATTTTCCTCGATCATGTCCTTAGATGACACCATTTTTCTATCTTGTTGATTCTTTGAATGCTCAGAATTATCAGAAATTCCAGAAATGAAACTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCCTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGGTATTTTCAATAATTCTTCCCTTTATCACACATTGTGCAAATTTTACTTTCACCTTAGGATTGCCTGTTGAACTCCAGATATTTATAGATGCCTTTAACTTTCGAGTTGCAAGTCTGAAGTGTTATGTAAGGCATATCTAGCATCATTTTTAATGATCCCATTCCCATTACTCAAAATCCAAACGTTTTTTACATTGTGACTGCACATGTGCTGATAGAATGTGGTTGTCTTTGCCTCTCATTTGATATTATAACTACATGTGATGTGACCGTTCTACATCAAGCGGTTGCAATTATAAGAACAATGTTCGATTTCTGAAAGATAGTTTAAACAATTCTGTGAGCGAATTTACCCGTCATTTGATGTGTTTGTATTGTACTTACGTAACTAACTTACTGTGTGGTTGCTCTGTAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATAGTTAAGTACTCTAACCCCTGCCATGTTCCACCTACGTTTTCCTTTCTACATTTTGTATCCATTTCCATATGCTCTTTATAGCTTTTATGTGCGTTCTTTTGTTGCTCTGTATTACATTTTGGTACCACTTGTTTATGTGACATTGGTTGTAACGTTGCTTTTTCATGTACAACTATTGACATTGAGGTAATTTCGTACTGTGATTTGCAGGTCACGTTGCTCACCTGAATATACATGACGAATTGCTTCCATATAAGGATGTCATTGCGAAGGTTATTTATGATGTATGTCATCAGTATCTATTTACTCATTTTCCTAATTATTTTGAAATGTACATTGTGATTTATTAATCACTATAATTTAGCTGTTAGTTCATTTTTTTATTCTTAATTTTGTTTTACTCTCCCCTTCTTTTCCTCCGTGCATATGTTGGGGCTACCTTTTTAAGAAATTCAAATGTTCAGAATCGGATATTTAATATTTTTATCCTCCCCCTTCATCCAGCAATATGCACACACACACATTTTCCATATTGAAACTTTTTCTGTAACTATTTCTTTTTGTAATCTTTGCAGAAAAATTATCCGAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTAAAGCAATATGGGGCAACTTTTAAGCTCGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCGTCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTATATAATTTGGATGCTAGGAAGTTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAATCTGTCACCTCAATACTAAAAGCTTGTGAGAAGGGCATTGGAGGGACCAATCAAGAATCACCATCGGCAAATGGTATGCCGACTGGTATTATATTATTATTATCATTTAAAGAAAATTTCACATCGTCTTCAGGAGATTAGTGTAGATGATCTTAATTTTCTTTCTTGCTTCAGTTCAGGTGCAAGAAGTTCAAGATTATATTAAAATTAGCAATAAATCTGTAGAACACTCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCAGAAAGTTGTGAGAACGAGAAAGGTAACATACTCTACCATAGTTTGTGATTGAACAACCAAGACCGTTTCTTCCTATAATTTTTTTTGCAGATTTTTTTTAATGAAAATTTGCTTTGCAGACAATGGAATTGCTGATGATGCTTCCACTCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCAGTGTATCTCATGCTAGGACCTGGGAACACGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGGTACGAATGACCTTTTTTCTTTTTAAATTTCATTTCACTTATTGCAGTTGTTTTAGCATATTTCTCTACATTTGTGTGTCAGAATGAAGAGTTAAAAGTTGGGGAAATAACAATGTTAGGAAAGGGAAACAATTACTTTTTAATGCTTCAAATATGAAGGGAAACCATAAACGATTTTTTCTCTCTTACTATCACCATGACAGATATTTTGGATATGTCATATTTCCTAATTCCTACCTTTCAAATGGAATTCTTTGTTGTAAGTTTTGTAGTCACTCTCTATACCAATTTTATGAGTTTCTTCAATCTCCTTTAGCTCTTGGGTGAGGGAGAATGCCTTATCTCCTCCACTCAATTATCTCAACTCGTGTTTTTTATCCATATCTATATTTTGCTTAGTTAACCAATGAAGTATATTTTGTTATTTTCTTGCCAGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAACCAAGATTTTATCATTTCGGTATGTTTATACTAAATTACTATAGATCCTTATTTGTCTGTCTTCTTTTTGATAATTGTATTTCTTTCTCACCATTCTATTTGGATATCTGTCTTGAAAAAAGTTGTCTTTCTTCTCATACCATCCTCTTTTAACTGTTCCACTCCAGGAGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGTATTGCTTTGTGTGTGTGTGTATAAATGGAAACTACTTGTTTTTAACTCTTTTTTCTTTTTTGTTTTATCAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTTGATGAAGACACGACTAGCTAAAATCGAGTAAAACTTTTCTATCTCTCGGGGCTTCATTTTCTTGTTCTCACATCGTCATAATTCATGTTTGTTTCTCATTTCTGAGATGTACAAGCTAGACGTATGATATCGTAGGCTAGCGCTTGGGTATTTCAGTTCTAAAGTTGAGATTGGTGTTTGGAAAATTTTCCAGATT

mRNA sequence

GCGACAGTTCCCGCTGTTAGTCTTCTACTACGGTGAAAATCTCGATTCTGATTCTGATTCTTTCTTTTCAGTGGAAAAATCTCCGAACCCATTACTCTACATTGTTCTATTCTGATTTGAATTTGTTTGCATTGAGTTGGGTGTAATTTTTGTTGTGTTATTGAGTATTGAGAAAAATCGAACTTGTGAATCTATCAATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCCTTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGAATTATCAGAAATTCCAGAAATGAAACTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCCTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATACTTTTATGTCACGTTGCTCACCTGAATATACATGACGAATTGCTTCCATATAAGGATGTCATTGCGAAGGTTATTTATGATAAAAATTATCCGAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTAAAGCAATATGGGGCAACTTTTAAGCTCGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCGTCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTATATAATTTGGATGCTAGGAAGTTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAATCTGTCACCTCAATACTAAAAGCTTGTGAGAAGGGCATTGGAGGGACCAATCAAGAATCACCATCGGCAAATGTTCAGGTGCAAGAAGTTCAAGATTATATTAAAATTAGCAATAAATCTGTAGAACACTCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCAGAAAGTTGTGAGAACGAGAAAGACAATGGAATTGCTGATGATGCTTCCACTCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCAGTGTATCTCATGCTAGGACCTGGGAACACGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAACCAAGATTTTATCATTTCGGAGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTTGATGAAGACACGACTAGCTAAAATCGAGTAAAACTTTTCTATCTCTCGGGGCTTCATTTTCTTGTTCTCACATCGTCATAATTCATGTTTGTTTCTCATTTCTGAGATGTACAAGCTAGACGTATGATATCGTAGGCTAGCGCTTGGGTATTTCAGTTCTAAAGTTGAGATTGGTGTTTGGAAAATTTTCCAGATT

Coding sequence (CDS)

ATGTTGGACGAAAGCAAATTCGACGTGCATCTGAATCTCTGGGCCTTAAGAATTCCGAGGGAACTTTGTAAGGCCGCCATTAAAATACTGAACGGGTACTTAATTGACAAGCCCCGGATTAAACCTGTGACTGAAGATCCAACATGTGATAAGAATCGTTATGTTATATTATCTGAAAAGGTTCAAACTCCAGAATTATCAGAAATTCCAGAAATGAAACTTGAAGAATTGAAAGGCTTATGCATGTTTGAACGAGTTCCCTATTCACTAACCCTTGGATATTCATATTGGGGAGCAGATCATATTCTCAAGAAGATTTTGCCTCCTGAAGTGGAAGTGCCTTCATCTTTTGAGACAATACTTTTATGTCACGTTGCTCACCTGAATATACATGACGAATTGCTTCCATATAAGGATGTCATTGCGAAGGTTATTTATGATAAAAATTATCCGAGGATTAAAACAGTTGTAAATAAAGTGGGTTCTATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAATACTGAAGGGAGAGAATGATATGGTAACAGAAGTAAAGCAATATGGGGCAACTTTTAAGCTCGACTACAGTTTGGTCTACTGGAACTCAAGATTGGAACATGAACACATAAGGCTGGTTTCACTCTTCCAACCAGGAGAGGTAATTTGTGACATGTTTGCTGGTATTGGTCCTTTTGCAATTCCAGCCGCCCAGAAAGAATGTATAGTATATGCCAATGACTTGAATCCAGACAGCGTCCGATATCTGAAGGTTAATGCAGAAATCAATAAAGTTAGTGGTCGCGTTCATGTATATAATTTGGATGCTAGGAAGTTTATTTCTCAATTGATGATAGTTCCCCCTAATGAAAGTAATTCAGAATCTGTCACCTCAATACTAAAAGCTTGTGAGAAGGGCATTGGAGGGACCAATCAAGAATCACCATCGGCAAATGTTCAGGTGCAAGAAGTTCAAGATTATATTAAAATTAGCAATAAATCTGTAGAACACTCAAGGAGTGCTGATATATCCGTTGCTGTTCTTAAAAGATCCTCAGAAAGTTGTGAGAACGAGAAAGACAATGGAATTGCTGATGATGCTTCCACTCGTGTAGCCGGTAGGAGAAATGGAAACGGAAATAAGAGGATGAAGGGGTCCAGTGTATCTCATGCTAGGACCTGGGAACACGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCTTTACATTTTCTTGATGCATTTAGAGGCTCAATTAAGAAGAAATACTGGAAGGGCTCCCTTCCTTGGATTCACTGTTACTGCTTCATGCGAGCAAATGAAAACCAAGATTTTATCATTTCGGAGGCTGAGTCTGCCTTGAATGCTAATATTCAAGACCCCATTTTTCATAGGGTCAGAGATGTTGCACCAAACAAGGCTATGTACTGTTTGAGCTTTAGGCTTCCGGAACAATGCGTTGATGAAGACACGACTAGCTAA

Protein sequence

MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKVQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETILLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILKACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSFRLPEQCVDEDTTS*

Homology

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BLAST of CSPI02G01360 vs. ExPASy Swiss-Prot
Match: F6HH45 (tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0016g04350 PE=3 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 7.6e-195
Identity = 343/501 (68.46%), Postives = 403/501 (80.44%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           +LDESKFD+ L LWALRIP +LCK A +ILNGYL+DKPR+KP+TEDPTC+ NRY+ILSE+
Sbjct: 2   LLDESKFDLQLKLWALRIPSQLCKVASRILNGYLLDKPRVKPITEDPTCETNRYIILSER 61

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           ++ P+LS+IPE K +ELK LC  E VPYSLTLGYSYW ADH+LK+ILP  VEVPSSFETI
Sbjct: 62  IKNPDLSDIPEDKRDELKVLCKIEVVPYSLTLGYSYWSADHVLKQILPDGVEVPSSFETI 121

Query: 121 L--LCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGE 180
              LCH+AHLNI  ELLPYKDVIAKVIYDKNYPRIKTVVNKVG+ITNEFRVPKFEIL G+
Sbjct: 122 TFSLCHIAHLNITGELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRVPKFEILVGK 181

Query: 181 NDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQ 240
           +DMVTEVKQY ATFKLDYSLVYWNSRLEHEH+RLVS F+PG++ICDMF+G+GPFAIPAAQ
Sbjct: 182 DDMVTEVKQYRATFKLDYSLVYWNSRLEHEHMRLVSQFRPGQIICDMFSGVGPFAIPAAQ 241

Query: 241 KECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTS 300
           K C+VYANDLNPDS+RYLK+NA+INKV   +  YN+DARKFISQLM VP +E   E    
Sbjct: 242 KGCLVYANDLNPDSIRYLKINAKINKVDDNIWAYNMDARKFISQLMEVPVHEILPEPDVP 301

Query: 301 ILKACEKGIGGTNQESPSAN----VQVQEVQDYIKISNKSVEHSRS-ADISVAVLKRSSE 360
           +LKA E+     N ++ S N    V+   V      +   +E S + AD +VA +K+ S 
Sbjct: 302 VLKATEECSIQANVQTESQNGGLTVEAIGVHSDDSSNMDGIEGSCTVADDTVAAVKKHSG 361

Query: 361 SCENEKDNGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFL 420
           SCE E  NG  D+A+  V GRR    NKRM+GS S S+ + WEHVDHVIMNLPASA+ FL
Sbjct: 362 SCEEE--NGTYDNATVSVVGRRKDRMNKRMRGSESSSNIKPWEHVDHVIMNLPASAIQFL 421

Query: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPN 480
           DAFRG I++KYWKGSLPWIHCYCF+RANE  + IISEAE+ALNA+IQ+PI HRVRDVAPN
Sbjct: 422 DAFRGLIQRKYWKGSLPWIHCYCFIRANETNEMIISEAETALNASIQEPILHRVRDVAPN 481

Query: 481 KAMYCLSFRLPEQ-CVDEDTT 493
           KAM+CLSFRL E+ C  ED T
Sbjct: 482 KAMFCLSFRLSEEACFKEDAT 500

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BLAST of CSPI02G01360 vs. ExPASy Swiss-Prot
Match: Q93YU6 (tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3g56120 PE=2 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 3.0e-175
Identity = 306/490 (62.45%), Postives = 374/490 (76.33%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+L LWALRIPRELCK+A +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           V+  +LSEIPE KL +LK L   E VP+S+TLGYSYW ADH+LK+ILP  +++PSSFETI
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              H+AHLN+HDELLP+KDVIAKVIYDKNYPRIKT+VNKVG+I+NEFRVPKFE+L GEN 
Sbjct: 121 --GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVLAGENG 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           M TEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGPFAIPAAQK 
Sbjct: 181 METEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGPFAIPAAQKG 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           C VYANDLNPDSVRYLK+NA+ NKV   + V+N+DARKF S LM V   E N +SV    
Sbjct: 241 CFVYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCEDNLQSVADND 300

Query: 301 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 360
           K  E  +    + + S         + I+ SN S+     A+      K+ S + + E  
Sbjct: 301 KTKEAAVSRGGETNSSG--------EEIRESNASINEPLGAN------KKPSGTTKTE-- 360

Query: 361 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420
           NG+  D  +       G+ NKR++ + +  A+ WEH+DHVIMNLPASAL FLD+F   I+
Sbjct: 361 NGVGKDCKS-----IEGHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDSFSNVIQ 420

Query: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 480
           KKYWKG LP IHCYCF+RA+E  +FII+EAE+AL  +I+DP+FH+VRDVAPNKAM+CLSF
Sbjct: 421 KKYWKGPLPLIHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMFCLSF 467

Query: 481 RLPEQCVDED 491
           RLPE C+ ++
Sbjct: 481 RLPEACLKQE 467

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BLAST of CSPI02G01360 vs. ExPASy Swiss-Prot
Match: C5XX79 (tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g025390 PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 8.2e-157
Identity = 287/490 (58.57%), Postives = 355/490 (72.45%), Query Frame = 0


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKIL-NGYLIDKPRIKPVTEDPTCDKNRYVILSEK 61
           LDESKF+  L LWALRIPREL  A  ++L +GYL+DKPR+K V EDP  DKNR VILSEK
Sbjct: 4   LDESKFEQRLQLWALRIPRELSSAVTRLLRSGYLLDKPRVKTVVEDPEGDKNRLVILSEK 63

Query: 62  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 121
           +Q P+LS++P+ +L+ LK LC  + VPY+LTLGYSYW A                     
Sbjct: 64  IQKPDLSDMPQQELDSLKQLCNVDVVPYTLTLGYSYWSAG-------------------- 123

Query: 122 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 181
              HVAHLNI D+LLPYK+VIAKVIYDKNYPRI+TV NKVG+ITNEFRVPKFEIL G+ND
Sbjct: 124 ---HVAHLNISDDLLPYKNVIAKVIYDKNYPRIQTVANKVGTITNEFRVPKFEILAGKND 183

Query: 182 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 241
           MVTEVKQYGATFKLDY LVYWNSRL+HEHIRLVSLF+ G+VICDMFAGIGPFAIPAAQK 
Sbjct: 184 MVTEVKQYGATFKLDYGLVYWNSRLDHEHIRLVSLFKKGDVICDMFAGIGPFAIPAAQKG 243

Query: 242 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 301
           C+VYANDLNPDSV YL+ NA+INKV   +  YN+DAR F+  LM VP  E+ S+   +  
Sbjct: 244 CVVYANDLNPDSVHYLRTNAKINKVDDYIFAYNMDARVFMQNLMTVPGLETGSDCQVAAD 303

Query: 302 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 361
           ++  K  G    E+ S+N    +V++    S      S  A  +    +++SE CE++  
Sbjct: 304 ESYPKE-GVPANENSSSNGNHNDVREG---SQNGANESSVASTTAKKRQQTSEECESDCQ 363

Query: 362 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 421
           +G  D + T+   RRN   NKR++G     ++ WEH DHV+MNLPASAL FLD F G ++
Sbjct: 364 DG--DASQTK---RRN---NKRVRGPGPPPSKPWEHFDHVLMNLPASALQFLDCFDGLVQ 423

Query: 422 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 481
           KKYW GSLPWIHCYCF+R++E+++ I+S   + LNA I +PIFHRVRDVAPNKAM+CLSF
Sbjct: 424 KKYWTGSLPWIHCYCFIRSSESEESILS---NKLNAKIAEPIFHRVRDVAPNKAMFCLSF 455

Query: 482 RLPEQCVDED 491
           +LP +C+ ED
Sbjct: 484 KLPMECLRED 455

[Close]

BLAST of CSPI02G01360 vs. ExPASy Swiss-Prot
Match: A9T6G5 (tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPADRAFT_141055 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.5e-113
Identity = 223/473 (47.15%), Postives = 295/473 (62.37%), Query Frame = 0


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKV 61
           +D+S F V L   ALRIP++       +L+ YL+D PR+K + EDP     R ++ SE +
Sbjct: 11  IDKSAFVVRLPRVALRIPKQKSHRVASLLSKYLLDMPRVKHIVEDPQDPSMRLLLFSEAI 70

Query: 62  QTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETIL 121
             P+L  + E K+  +K +   + V + + L YSYW  +HILK+ILP   EVPSSFETI 
Sbjct: 71  AQPDLEGVSEEKILAVKEIVPLDVVQHEVVLDYSYWPVEHILKEILPAGCEVPSSFETI- 130

Query: 122 LCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDM 181
             H+AHLN+ ++LL YK +IA+VI DKN P++KTVVNKVG+ITNEFRVP+FEIL GE  +
Sbjct: 131 -GHIAHLNLREDLLTYKKIIAEVILDKN-PKLKTVVNKVGTITNEFRVPEFEILAGEPSL 190

Query: 182 VTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKEC 241
           VTE+KQ+GATF+LDY +VYWNSRLE EH RL + F+PG+VI DMFAGIGPFAIPAAQ+ C
Sbjct: 191 VTEIKQHGATFRLDYGMVYWNSRLEGEHKRLFAQFKPGQVIVDMFAGIGPFAIPAAQQGC 250

Query: 242 IVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSILK 301
            VYANDLNP SV++LK+N++INKV   +  +NLDAR+F+ +L  V   E     + S   
Sbjct: 251 AVYANDLNPTSVKFLKLNSDINKVGESIKAFNLDAREFMRKL--VTEEEGCVGEIASSTL 310

Query: 302 ACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKDN 361
             E  + GT    PS+                               K +S S     D 
Sbjct: 311 IAENSLNGT--RIPSS-------------------------------KENSSSLTEGSDM 370

Query: 362 GIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIKK 421
             +     R A +   +   +MK   +S  + WEH DHV+MNLPASAL FLD   G + K
Sbjct: 371 PSSKKPEERKAKKAKVSEVVKMK---ISDVKPWEHFDHVVMNLPASALEFLDVLNGLLCK 430

Query: 422 KYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKA 475
             WKG++P +HCYCFMR+NE    I+ +AE+ L  +I DP  + VRDVAPNKA
Sbjct: 431 GRWKGTMPRVHCYCFMRSNETNADIVKKAETYLGGSIADPDVYVVRDVAPNKA 442

[Close]

BLAST of CSPI02G01360 vs. ExPASy Swiss-Prot
Match: F4NUJ6 (tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strain JAM81 / FGSC 10211) OX=684364 GN=TRM5 PE=3 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.6e-75
Identity = 168/488 (34.43%), Postives = 262/488 (53.69%), Query Frame = 0


Query: 2   LDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEKV 61
           L+ + F+    L AL+IP      A++ L  +++  PR++ + +DPT  K R ++L   +
Sbjct: 16  LNHALFESSHTLPALKIPAHASGIAMETLRSHILVAPRLRTIVDDPTDKKWRLLLLDPSM 75

Query: 62  QTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETIL 121
           +  E++++P   L+E       + V +++ L Y YW +D +L+ ILP E+E P +FET+ 
Sbjct: 76  EASEINDLPN-PLKEFALKHEAKLVKHTIELKYDYWTSDQVLRSILPDEMETPGAFETV- 135

Query: 122 LCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGENDM 181
             H+AHLN+ D   P+K +I +VI DK+   IKTVVNK+ +I + FR  + EIL G NDM
Sbjct: 136 -GHIAHLNLRDRYQPFKHIIGQVILDKS-SHIKTVVNKLDNIDHTFRFFQMEILAGINDM 195

Query: 182 VTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQ-KE 241
             ++K+ G  F  D+S VYWNSRL+ EH R++ LF   ++ICD+FAG+GPFA+PAA+ K 
Sbjct: 196 NAKLKEGGCFFHFDFSKVYWNSRLQGEHDRIIKLFGQNDLICDVFAGVGPFALPAAKHKR 255

Query: 242 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 301
           C+V+ANDLNP S +YL  N ++NK+  R+  +N+D R+FI Q +     + N+ ++ + +
Sbjct: 256 CVVFANDLNPQSFKYLMENIKLNKLETRILPFNMDGRQFIKQSL----EDLNNPAIWNKI 315

Query: 302 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 361
                      ++ P++N          K  N+ VE    A ++           +    
Sbjct: 316 ----------TKQKPTSN---------DKKRNRKVESPTVAPLT-----------DQPAI 375

Query: 362 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRG--- 421
           +GI                                H  H +MNLPA+A+ FLDAF G   
Sbjct: 376 SGI-------------------------------RHFKHYVMNLPATAIEFLDAFHGLYS 434

Query: 422 SIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIF--HRVRDVAPNKAM 481
            ++       LP IHC+CF  A + +  +I   E  +   +   +   H VR VAPNK M
Sbjct: 436 GMRDVIMDSDLPTIHCHCFSNAKDVKADVIERVERVIGMPLGSNLIMVHSVRTVAPNKDM 434

Query: 482 YCLSFRLP 484
            C+SFRLP
Sbjct: 496 LCISFRLP 434

[Close]

BLAST of CSPI02G01360 vs. ExPASy TrEMBL
Match: A0A0A0LFW8 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G005350 PE=3 SV=1)

HSP 1 Score: 996.5 bits (2575), Expect = 3.9e-287
Identity = 490/493 (99.39%), Postives = 491/493 (99.59%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
           LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND
Sbjct: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE
Sbjct: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           CIVYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL
Sbjct: 241 CIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300

Query: 301 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 360
           KACEKGIGGTNQESP ANVQVQEVQDYIKISNKSVE SRSADISVAVLKRSSESCENEKD
Sbjct: 301 KACEKGIGGTNQESPLANVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKD 360

Query: 361 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420
           NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK
Sbjct: 361 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420

Query: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 480
           KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF
Sbjct: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 480

Query: 481 RLPEQCVDEDTTS 494
           RLPEQCVDEDTTS
Sbjct: 481 RLPEQCVDEDTTS 493

[Close]

BLAST of CSPI02G01360 vs. ExPASy TrEMBL
Match: A0A5A7V0B1 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G001350 PE=3 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 2.9e-274
Identity = 479/501 (95.61%), Postives = 487/501 (97.21%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 ----LLC---HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180
               +LC   HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE
Sbjct: 121 GDNFVLCFAGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180

Query: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240
           ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA
Sbjct: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240

Query: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300
           IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS
Sbjct: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300

Query: 301 ESVTSILKACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSE 360
           E+VTSILKA E+GIGGTNQESP ANVQVQEVQD I+ISNK VEHSRSADISVAVLKRSSE
Sbjct: 301 ETVTSILKASEEGIGGTNQESPLANVQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSE 360

Query: 361 SCENEKDNGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFL 420
           SCENEKD+ IADDASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALHFL
Sbjct: 361 SCENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFL 420

Query: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPN 480
           DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVAPN
Sbjct: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPN 480

Query: 481 KAMYCLSFRLPEQCVDEDTTS 494
           KAMYCLSFRLPEQCV+EDTTS
Sbjct: 481 KAMYCLSFRLPEQCVNEDTTS 500

[Close]

BLAST of CSPI02G01360 vs. ExPASy TrEMBL
Match: A0A1S3C7L3 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103497610 PE=3 SV=1)

HSP 1 Score: 952.2 bits (2460), Expect = 8.5e-274
Identity = 476/494 (96.36%), Postives = 484/494 (97.98%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND
Sbjct: 121 --GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE
Sbjct: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFIS+LMIVPPNESNSE+VTSIL
Sbjct: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISRLMIVPPNESNSETVTSIL 300

Query: 301 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 360
           KA E+GIGGTNQESP ANVQVQEVQD I+ISNK VEHSRSADISVAVLKRSSESCENEKD
Sbjct: 301 KASEEGIGGTNQESPLANVQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESCENEKD 360

Query: 361 NGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFLDAFRGSI 420
           + IADDASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALHFLDAFRGSI
Sbjct: 361 DRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSI 420

Query: 421 KKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLS 480
           KKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVAPNKAMYCLS
Sbjct: 421 KKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLS 480

Query: 481 FRLPEQCVDEDTTS 494
           FRLPEQCV+EDTTS
Sbjct: 481 FRLPEQCVNEDTTS 491

[Close]

BLAST of CSPI02G01360 vs. ExPASy TrEMBL
Match: A0A5D3CFY1 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00100 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 7.7e-259
Identity = 455/494 (92.11%), Postives = 463/494 (93.72%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGA                     
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGAG-------------------- 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND
Sbjct: 121 ---HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE
Sbjct: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSE+VTSIL
Sbjct: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSETVTSIL 300

Query: 301 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 360
           KA E+GIGGTNQESP ANVQVQEVQD I+ISNK VEHSRSADISVAVLKRSSESCENEK+
Sbjct: 301 KASEEGIGGTNQESPLANVQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESCENEKE 360

Query: 361 NGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFLDAFRGSI 420
           + IADDASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALHFLDAFRGSI
Sbjct: 361 DRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSI 420

Query: 421 KKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLS 480
           KKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVAPNKAMYCLS
Sbjct: 421 KKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLS 470

Query: 481 FRLPEQCVDEDTTS 494
           FRLPEQCV+EDTTS
Sbjct: 481 FRLPEQCVNEDTTS 470

[Close]

BLAST of CSPI02G01360 vs. ExPASy TrEMBL
Match: A0A6J1DUW2 (tRNA (guanine(37)-N1)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111024249 PE=3 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 5.4e-252
Identity = 430/489 (87.93%), Postives = 458/489 (93.66%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHL LWALRIPRELCKAAIKILNGYLID+ R+KPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKAAIKILNGYLIDRARVKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQ PELS+IPE KLEELKGLC  E VPYSLTLGYSYWGADHILK+ILPP VEVPSSFETI
Sbjct: 61  VQAPELSQIPEAKLEELKGLCEIELVPYSLTLGYSYWGADHILKQILPPRVEVPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              H+AHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVG+ITN+FRVPKFEIL GE+D
Sbjct: 121 --GHIAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNKFRVPKFEILMGEHD 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDY LVYWNSRLEHEH+RLVSLFQPGE+ICDMFAGIGPFAIPAAQKE
Sbjct: 181 MVTEVKQYGATFKLDYGLVYWNSRLEHEHVRLVSLFQPGEIICDMFAGIGPFAIPAAQKE 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           CIVY NDLNPDSV+YLKVNAEINKVS RVHVYNLDARKFISQLMIVP NESNSESVTS+L
Sbjct: 241 CIVYGNDLNPDSVQYLKVNAEINKVSDRVHVYNLDARKFISQLMIVPDNESNSESVTSVL 300

Query: 301 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 360
           KAC+KGIGG+NQESPSANVQV+EVQ+  KIS+++VEHSRSADISV+VLKRSSESC  EKD
Sbjct: 301 KACDKGIGGSNQESPSANVQVEEVQENSKISDETVEHSRSADISVSVLKRSSESC--EKD 360

Query: 361 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420
           +GI DDAS  VAGR+  N NKRMKGS++ HARTWEHVDHVIMNLPASAL FLDAFRGSI+
Sbjct: 361 DGIVDDASISVAGRKKRNANKRMKGSNICHARTWEHVDHVIMNLPASALQFLDAFRGSIR 420

Query: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 480
           +KYWKGSLPWIHCYCFMRANENQDFIISEAESALN  IQDP+FHRVRDVAPNKAMYCLSF
Sbjct: 421 RKYWKGSLPWIHCYCFMRANENQDFIISEAESALNGKIQDPVFHRVRDVAPNKAMYCLSF 480

Query: 481 RLPEQCVDE 490
           RLPEQC +E
Sbjct: 481 RLPEQCFNE 485

[Close]

BLAST of CSPI02G01360 vs. NCBI nr
Match: XP_011648665.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 984.2 bits (2543), Expect = 4.2e-283
Identity = 487/493 (98.78%), Postives = 488/493 (98.99%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND
Sbjct: 121 --GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE
Sbjct: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           CIVYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL
Sbjct: 241 CIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300

Query: 301 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 360
           KACEKGIGGTNQESP ANVQVQEVQDYIKISNKSVE SRSADISVAVLKRSSESCENEKD
Sbjct: 301 KACEKGIGGTNQESPLANVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCENEKD 360

Query: 361 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420
           NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK
Sbjct: 361 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420

Query: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 480
           KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF
Sbjct: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 480

Query: 481 RLPEQCVDEDTTS 494
           RLPEQCVDEDTTS
Sbjct: 481 RLPEQCVDEDTTS 491

[Close]

BLAST of CSPI02G01360 vs. NCBI nr
Match: XP_031736378.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 978.4 bits (2528), Expect = 2.3e-281
Identity = 487/497 (97.99%), Postives = 488/497 (98.19%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND
Sbjct: 121 --GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE
Sbjct: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           CIVYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL
Sbjct: 241 CIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300

Query: 301 KACEKGIGGTNQESPSAN----VQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCE 360
           KACEKGIGGTNQESP AN    VQVQEVQDYIKISNKSVE SRSADISVAVLKRSSESCE
Sbjct: 301 KACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSSESCE 360

Query: 361 NEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFR 420
           NEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFR
Sbjct: 361 NEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFR 420

Query: 421 GSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMY 480
           GSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMY
Sbjct: 421 GSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMY 480

Query: 481 CLSFRLPEQCVDEDTTS 494
           CLSFRLPEQCVDEDTTS
Sbjct: 481 CLSFRLPEQCVDEDTTS 495

[Close]

BLAST of CSPI02G01360 vs. NCBI nr
Match: KAA0061573.1 (tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 953.7 bits (2464), Expect = 6.0e-274
Identity = 479/501 (95.61%), Postives = 487/501 (97.21%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 ----LLC---HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180
               +LC   HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE
Sbjct: 121 GDNFVLCFAGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFE 180

Query: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240
           ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA
Sbjct: 181 ILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFA 240

Query: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300
           IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS
Sbjct: 241 IPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNS 300

Query: 301 ESVTSILKACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSE 360
           E+VTSILKA E+GIGGTNQESP ANVQVQEVQD I+ISNK VEHSRSADISVAVLKRSSE
Sbjct: 301 ETVTSILKASEEGIGGTNQESPLANVQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSE 360

Query: 361 SCENEKDNGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFL 420
           SCENEKD+ IADDASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALHFL
Sbjct: 361 SCENEKDDRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFL 420

Query: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPN 480
           DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVAPN
Sbjct: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPN 480

Query: 481 KAMYCLSFRLPEQCVDEDTTS 494
           KAMYCLSFRLPEQCV+EDTTS
Sbjct: 481 KAMYCLSFRLPEQCVNEDTTS 500

[Close]

BLAST of CSPI02G01360 vs. NCBI nr
Match: XP_008458074.1 (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo])

HSP 1 Score: 952.2 bits (2460), Expect = 1.8e-273
Identity = 476/494 (96.36%), Postives = 484/494 (97.98%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              HVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND
Sbjct: 121 --GHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE
Sbjct: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFIS+LMIVPPNESNSE+VTSIL
Sbjct: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISRLMIVPPNESNSETVTSIL 300

Query: 301 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 360
           KA E+GIGGTNQESP ANVQVQEVQD I+ISNK VEHSRSADISVAVLKRSSESCENEKD
Sbjct: 301 KASEEGIGGTNQESPLANVQVQEVQD-IEISNKFVEHSRSADISVAVLKRSSESCENEKD 360

Query: 361 NGIADDASTRVAGRRNGNGNKRMKGS-SVSHARTWEHVDHVIMNLPASALHFLDAFRGSI 420
           + IADDASTRVAGRRNGNGNKRMKGS +VSHARTWEH+DHVIMNLPASALHFLDAFRGSI
Sbjct: 361 DRIADDASTRVAGRRNGNGNKRMKGSTNVSHARTWEHIDHVIMNLPASALHFLDAFRGSI 420

Query: 421 KKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLS 480
           KKKYWKGSLPWIHCYCFMRANENQDFIIS AESALNANIQDPIFHRVRDVAPNKAMYCLS
Sbjct: 421 KKKYWKGSLPWIHCYCFMRANENQDFIISVAESALNANIQDPIFHRVRDVAPNKAMYCLS 480

Query: 481 FRLPEQCVDEDTTS 494
           FRLPEQCV+EDTTS
Sbjct: 481 FRLPEQCVNEDTTS 491

[Close]

BLAST of CSPI02G01360 vs. NCBI nr
Match: KAE8651523.1 (hypothetical protein Csa_019510 [Cucumis sativus])

HSP 1 Score: 925.2 bits (2390), Expect = 2.3e-265
Identity = 466/501 (93.01%), Postives = 470/501 (93.81%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI
Sbjct: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120

Query: 121 LL----CHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILK 180
           +     CHV                   ++ KNYPRIKTVVNKVGSITNEFRVPKFEILK
Sbjct: 121 VKYSNPCHV-------------PPTLSFLHFKNYPRIKTVVNKVGSITNEFRVPKFEILK 180

Query: 181 GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA 240
           GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA
Sbjct: 181 GENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPA 240

Query: 241 AQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESV 300
           AQKECIVYANDLNPDS+RYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESV
Sbjct: 241 AQKECIVYANDLNPDSIRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESV 300

Query: 301 TSILKACEKGIGGTNQESPSAN----VQVQEVQDYIKISNKSVEHSRSADISVAVLKRSS 360
           TSILKACEKGIGGTNQESP AN    VQVQEVQDYIKISNKSVE SRSADISVAVLKRSS
Sbjct: 301 TSILKACEKGIGGTNQESPLANGMPTVQVQEVQDYIKISNKSVEQSRSADISVAVLKRSS 360

Query: 361 ESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFL 420
           ESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFL
Sbjct: 361 ESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFL 420

Query: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPN 480
           DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPN
Sbjct: 421 DAFRGSIKKKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPN 480

Query: 481 KAMYCLSFRLPEQCVDEDTTS 494
           KAMYCLSFRLPEQCVDEDTTS
Sbjct: 481 KAMYCLSFRLPEQCVDEDTTS 488

[Close]

BLAST of CSPI02G01360 vs. TAIR 10
Match: AT3G56120.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 616.3 bits (1588), Expect = 2.1e-176
Identity = 306/490 (62.45%), Postives = 374/490 (76.33%), Query Frame = 0


Query: 1   MLDESKFDVHLNLWALRIPRELCKAAIKILNGYLIDKPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+L LWALRIPRELCK+A +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQTPELSEIPEMKLEELKGLCMFERVPYSLTLGYSYWGADHILKKILPPEVEVPSSFETI 120
           V+  +LSEIPE KL +LK L   E VP+S+TLGYSYW ADH+LK+ILP  +++PSSFETI
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 LLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEILKGEND 180
              H+AHLN+HDELLP+KDVIAKVIYDKNYPRIKT+VNKVG+I+NEFRVPKFE+L GEN 
Sbjct: 121 --GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRVPKFEVLAGENG 180

Query: 181 MVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMFAGIGPFAIPAAQKE 240
           M TEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGIGPFAIPAAQK 
Sbjct: 181 METEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGIGPFAIPAAQKG 240

Query: 241 CIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIVPPNESNSESVTSIL 300
           C VYANDLNPDSVRYLK+NA+ NKV   + V+N+DARKF S LM V   E N +SV    
Sbjct: 241 CFVYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTCEDNLQSVADND 300

Query: 301 KACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVAVLKRSSESCENEKD 360
           K  E  +    + + S         + I+ SN S+     A+      K+ S + + E  
Sbjct: 301 KTKEAAVSRGGETNSSG--------EEIRESNASINEPLGAN------KKPSGTTKTE-- 360

Query: 361 NGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPASALHFLDAFRGSIK 420
           NG+  D  +       G+ NKR++ + +  A+ WEH+DHVIMNLPASAL FLD+F   I+
Sbjct: 361 NGVGKDCKS-----IEGHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDSFSNVIQ 420

Query: 421 KKYWKGSLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFHRVRDVAPNKAMYCLSF 480
           KKYWKG LP IHCYCF+RA+E  +FII+EAE+AL  +I+DP+FH+VRDVAPNKAM+CLSF
Sbjct: 421 KKYWKGPLPLIHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMFCLSF 467

Query: 481 RLPEQCVDED 491
           RLPE C+ ++
Sbjct: 481 RLPEACLKQE 467

[Close]

BLAST of CSPI02G01360 vs. TAIR 10
Match: AT4G27340.1 (Met-10+ like family protein )

HSP 1 Score: 213.8 bits (543), Expect = 3.2e-55
Identity = 140/440 (31.82%), Postives = 201/440 (45.68%), Query Frame = 0


Query: 53  RYVILSEKVQTPELSEIPE------MKLEELKGLCMFERVPYSLTLGYSYWGADHILKKI 112
           R ++L EK    E+ ++PE       + +       FE V   LTL Y YW    IL+ +
Sbjct: 274 RLLLLDEKYSGEEVQDLPEAIKVLFAEAKMADASLSFELVKCRLTLFYDYWPMIEILEAV 333

Query: 113 LPPEVEVPSSFETILLCHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGSITNE 172
           LP  + VPS+FE  ++ H+AHLN+ DE LPYK +IAKV+ DKN P+I+TVVNK+  I N+
Sbjct: 334 LPKGMIVPSAFE--MVGHIAHLNLRDEHLPYKRLIAKVVLDKNQPKIQTVVNKIDPIHND 393

Query: 173 FRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICDMF 232
           FR  + E+L G + +VT V + G  F +D + VYWNS+L  E  RL+  F   +V+CD+F
Sbjct: 394 FRTMQLEVLAGNHSLVTLVVENGLRFHVDLARVYWNSKLGTERQRLLLGFDQNDVVCDVF 453

Query: 233 AGIGPFAIPAAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDARKFISQLMIV 292
           AG+GP A+ AA+    VYANDLNP +V +++ N+ +NK+  R+ ++N+D R+FI  +   
Sbjct: 454 AGVGPIALAAARIVKRVYANDLNPHAVEFMEQNSVVNKLEKRIEIFNMDGRRFIKAMF-- 513

Query: 293 PPNESNSESVTSILKACEKGIGGTNQESPSANVQVQEVQDYIKISNKSVEHSRSADISVA 352
                          + EKG                                        
Sbjct: 514 ---------------SSEKG---------------------------------------- 573

Query: 353 VLKRSSESCENEKDNGIADDASTRVAGRRNGNGNKRMKGSSVSHARTWEHVDHVIMNLPA 412
                                                           + V  V+MNLP 
Sbjct: 574 ------------------------------------------------QKVTQVVMNLPK 606

Query: 413 SALHFLDAFRGSIKKKYWKG--SLPWIHCYCFMRANENQDFIISEAESALNANIQDPIFH 472
            A   LDAFRG    ++     S P IH Y F +A++ +         AL+    D    
Sbjct: 634 DAAESLDAFRGVYNDRHRDEGLSFPTIHVYGFSKASDPEFDFHERIRIALSEVAVDVKMR 606

Query: 473 RVRDVAPNKAMYCLSFRLPE 485
           +VR VAP K M C SF LP+
Sbjct: 694 KVRLVAPGKWMLCASFILPK 606

[Close]

BLAST of CSPI02G01360 vs. TAIR 10
Match: AT4G04670.1 (Met-10+ like family protein / kelch repeat-containing protein )

HSP 1 Score: 56.6 bits (135), Expect = 6.5e-08
Identity = 37/115 (32.17%), Postives = 57/115 (49.57%), Query Frame = 0


Query: 165 NEFRVPKFEILKGENDMVTEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEVICD 224
           N  R    EIL G+N  V   ++ G  +  D +   ++     E +R+ ++    EV+ D
Sbjct: 785 NGTRDSTLEILVGDNGWVNH-RENGILYSFDATKCMFSWGNLSEKLRMGNMACENEVVVD 844

Query: 225 MFAGIGPFAIP--AAQKECIVYANDLNPDSVRYLKVNAEINKVSGRVHVYNLDAR 278
           +FAGIG F +P     K  +VYA + NP ++  L+ N E N VS R  +   D R
Sbjct: 845 LFAGIGYFVLPFLVRAKAKLVYACEWNPHAIEALRRNVEANSVSERCIILEGDNR 898

The following BLAST results are available for this feature:

BLAST of CSPI02G01360 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F6HH45	7.6e-195	68.46	tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0...	[more]
Q93YU6	3.0e-175	62.45	tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3...	[more]
C5XX79	8.2e-157	58.57	tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g02539...	[more]
A9T6G5	1.5e-113	47.15	tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPA...	[more]
F4NUJ6	1.6e-75	34.43	tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strai...	[more]

BLAST of CSPI02G01360 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFW8	3.9e-287	99.39	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G0053...	[more]
A0A5A7V0B1	2.9e-274	95.61	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3C7L3	8.5e-274	96.36	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103497610 ...	[more]
A0A5D3CFY1	7.7e-259	92.11	tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A6J1DUW2	5.4e-252	87.93	tRNA (guanine(37)-N1)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of CSPI02G01360 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011648665.1	4.2e-283	98.78	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucumis sativus]	[more]
XP_031736378.1	2.3e-281	97.99	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucumis sativus]	[more]
KAA0061573.1	6.0e-274	95.61	tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo var. makuwa]	[more]
XP_008458074.1	1.8e-273	96.36	PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 2 [Cucumis melo]	[more]
KAE8651523.1	2.3e-265	93.01	hypothetical protein Csa_019510 [Cucumis sativus]	[more]

BLAST of CSPI02G01360 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56120.1	2.1e-176	62.45	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G27340.1	3.2e-55	31.82	Met-10+ like family protein	[more]
AT4G04670.1	6.5e-08	32.17	Met-10+ like family protein / kelch repeat-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.300.110		coord: 100..179 e-value: 8.9E-20 score: 72.5
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 182..482 e-value: 4.7E-58 score: 197.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 357..388
None	No IPR available	PANTHER	PTHR23245:SF36	TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE	coord: 2..487
None	No IPR available	PANTHER	PTHR23245	TRNA METHYLTRANSFERASE	coord: 2..487
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 222..279 e-value: 4.04112E-4 score: 37.7947
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PFAM	PF02475	Met_10	coord: 123..434 e-value: 4.8E-63 score: 212.5
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PROSITE	PS51684	SAM_MT_TRM5_TYW2	coord: 117..484 score: 104.446381
IPR025792	tRNA (guanine(37)-N(1))-methyltransferase, eukaryotic	HAMAP	MF_03152	TRM5	coord: 2..484 score: 25.421278
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 114..484

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G01360.1	CSPI02G01360.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
cellular_component	GO:0005759	mitochondrial matrix
cellular_component	GO:0005634	nucleus
molecular_function	GO:0052906	tRNA (guanine(37)-N(1))-methyltransferase activity
molecular_function	GO:0009019	tRNA (guanine-N1-)-methyltransferase activity