CSPI01G25210 (gene) Cucumber (PI 183967) v1

BLAST of CSPI01G25210 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 5.1e-147
Identity = 261/538 (48.51%), Postives = 365/538 (67.84%), Query Frame = 0

Query: 13  LIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDY-GSSSTNPIANLIYTPTNSSYSSIL 72
           +I F +   + +S A P        E+F++CL  Y  ++ TN  A L+YT  +  Y SIL
Sbjct: 14  IIFFFLSFNIQISIANP-------QENFLKCLSQYIPTNVTN--AKLVYTQHDQFYMSIL 73

Query: 73  NFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISD 132
           N ++QNLRF +   PKP  I TPL+VSHIQ  ++CSK  G QIRTRSGGHD EG+SYIS 
Sbjct: 74  NSTVQNLRFTSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQ 133

Query: 133 IPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGH 192
           +PFV+VDL N+ S+ IDV +Q AWV SGATLGE+YY I+E ++ L+FPAG CPTVG GGH
Sbjct: 134 VPFVIVDLRNMHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGH 193

Query: 193 LSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAW 252
            SGGGYG L+R YGLAAD++IDA+LV+ +GKV +++SMGEDLFWAIRGGGG +FGI+ AW
Sbjct: 194 FSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAW 253

Query: 253 KVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE-E 312
           K++LV VP+  TI    + +E   ++KL+++WQ +A   +  LLL        +T+++ +
Sbjct: 254 KIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGK 313

Query: 313 GKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVL----LMGWFQKE 372
            K    + FSS+F G  + ++ ++  +FP+LG+ K DC ++SWI +++    ++ +    
Sbjct: 314 NKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTN 373

Query: 373 DPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMS 432
              E+LL+RS        IK DYVK+ IP  A+  + E+L  +DV +   +F PYGG M 
Sbjct: 374 FKKEILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMD 433

Query: 433 EVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYV 492
           E+ +S  PF HR+  +Y + YIA W+    E  + H+NWIR +Y++ TP+VS +PR AY+
Sbjct: 434 EISESAIPFPHRAGITYEIWYIASWE--KQEDNEKHINWIRNVYNFTTPYVSQNPRMAYL 493

Query: 493 NYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 545
           NYRDLD+G  N     +Y +AR+WG KYFGKNF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 494 NYRDLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of CSPI01G25210 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.1e-146
Identity = 263/542 (48.52%), Postives = 365/542 (67.34%), Query Frame = 0

Query: 13  LIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDY-GSSSTNPIANLIYTPTNSSYSSIL 72
           +I F +   + +S A P        E+F++C   Y  ++ TN  A L+YT  +  Y SIL
Sbjct: 14  IIFFFLSFNIQISIANP-------QENFLKCFSQYIPTNVTN--AKLVYTQHDQFYMSIL 73

Query: 73  NFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISD 132
           N +IQNLRF +   PKP  IITPL+VSHIQ  ++CSK  G QIRTRSGGHD EG+SYIS 
Sbjct: 74  NSTIQNLRFTSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQ 133

Query: 133 IPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGH 192
           +PFV+VDL N+ S+ IDV +Q AWV +GATLGE+YY I+E ++ L+FPAG CPTVG GGH
Sbjct: 134 VPFVIVDLRNMHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGH 193

Query: 193 LSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAW 252
            SGGGYG L+R YGLAAD++IDA+LV+ +GKV +++SMGEDLFWAIRGGGG +FGI+ AW
Sbjct: 194 FSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAW 253

Query: 253 KVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE-E 312
           K++LV VP+  TI    + +E   ++KL+++WQ +A   +  LLL        +T+++ +
Sbjct: 254 KIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGK 313

Query: 313 GKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMG--------W 372
            K    + FSS+F G  + ++ ++  +FP+LG+ K DC ++SWI +++           +
Sbjct: 314 NKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTY 373

Query: 373 FQKEDPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYG 432
           F+K    E+LL+RS        IK DYVK+ IP  A+  + E+L  +DV +   +F PYG
Sbjct: 374 FKK----EILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYG 433

Query: 433 GKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPR 492
           G M E+ +S  PF HR+   Y + YIA W+    E  + H+NWIR +Y++ TP+VS +PR
Sbjct: 434 GIMDEISESAIPFPHRAGIMYEIWYIASWE--KQEDNEKHINWIRNVYNFTTPYVSQNPR 493

Query: 493 AAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIP 545
            AY+NYRDLD+G  N     +Y +AR+WG KYFGKNF+RLV VKTKVDP +FFR+EQSIP
Sbjct: 494 MAYLNYRDLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIP 540

BLAST of CSPI01G25210 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 4.8e-145
Identity = 261/539 (48.42%), Postives = 360/539 (66.79%), Query Frame = 0

Query: 13  LIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIAN--LIYTPTNSSYSSI 72
           +I F +   + +S A P        E+F++C   +     N +AN  L+YT  +  Y SI
Sbjct: 14  IIFFFLSFHIQISIANP-------RENFLKC---FSKHIPNNVANPKLVYTQHDQLYMSI 73

Query: 73  LNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYIS 132
           LN +IQNLRF++   PKP  I+TP + SHIQA ++CSK  G QIRTRSGGHD EG+SYIS
Sbjct: 74  LNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYIS 133

Query: 133 DIPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGG 192
            +PFVVVDL N+ SI IDV +Q AWV +GATLGE+YY I+EK++ L+FP G CPTVGVGG
Sbjct: 134 QVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGG 193

Query: 193 HLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVA 252
           H SGGGYG L+R YGLAAD++IDA+LV+ +GKV +++SMGEDLFWAIRGGGG +FGI+ A
Sbjct: 194 HFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAA 253

Query: 253 WKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE- 312
           WK+KLV VP++ TI    + +E   ++KL ++WQ +A K D +L+L        +T++  
Sbjct: 254 WKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHG 313

Query: 313 EGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVL----LMGWFQK 372
           + K      FSS+F G  + ++ ++  +FP+LG+ K DC E SWI + +    ++ +   
Sbjct: 314 KNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTA 373

Query: 373 EDPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKM 432
               E+LL+RS        IK DYVK+ IP  A+  + E+L  +DV     +  PYGG M
Sbjct: 374 NFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIM 433

Query: 433 SEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAY 492
            E+ +S  PF HR+   Y + Y A W+    E  + H+NW+R +Y++ TP+VS +PR AY
Sbjct: 434 EEISESAIPFPHRAGIMYELWYTASWE--KQEDNEKHINWVRSVYNFTTPYVSQNPRLAY 493

Query: 493 VNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 545
           +NYRDLD+G  N     +Y +AR+WG KYFGKNF+RLV VKTKVDP++FFR+EQSIP L
Sbjct: 494 LNYRDLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of CSPI01G25210 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 6.2e-145
Identity = 253/532 (47.56%), Postives = 359/532 (67.48%), Query Frame = 0

Query: 13  LIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILN 72
           ++ F     ++LS   P   +     SF+QCL     + +N ++ +I+TP ++S+SS+L 
Sbjct: 10  VLIFFTTSTLLLSIPHPVSANRSNQSSFLQCL-SLQLNDSNIVSKVIHTPNDTSFSSVLA 69

Query: 73  FSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDI 132
            SIQN RF   D+PKP  I+TP+  S +Q+AV C++  G  IRTRSGGHD+EGLSY++  
Sbjct: 70  SSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHK 129

Query: 133 PFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHL 192
           PFV++DL NL+SI++DV+N++ WV +GAT+GELYY I +K++TLAFPAG CPTVGVGGH 
Sbjct: 130 PFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHF 189

Query: 193 SGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWK 252
           SGGGYG L+RK+GLAADHVIDA +VDA G++  +  MGED FWAIRGGGG SF +V++WK
Sbjct: 190 SGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWK 249

Query: 253 VKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGK 312
           + L+ VP+ VT+    +   E   LK+IH WQ+VA K+  +L + + L        +  K
Sbjct: 250 IGLINVPSTVTVFNVTK-FSEQSALKIIHRWQFVADKVSDDLFIRVML--------QRYK 309

Query: 313 INPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWFQK--EDPLE 372
               ASF  ++LG  + +L ++   FP+LGL ++DC EMSWI+SV+   WF +  E+P+ 
Sbjct: 310 NMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVI---WFAELGEEPIN 369

Query: 373 VLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDD 432
           VL  R+R  S   K KSD+V+E +P  A+  +W RL+  + E +Q++F P+GGKMSE+ D
Sbjct: 370 VLTKRTRA-SLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIAD 429

Query: 433 SETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYRD 492
            ETPF HR    Y + Y+  W+    + ++ ++ W+  +YD M+ FV+ SPR AY+N RD
Sbjct: 430 YETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRD 489

Query: 493 LDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIP 543
           LD+G      R+ Y+  + WG+KYF  NF+RLV VKT VDPSDFF  EQSIP
Sbjct: 490 LDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of CSPI01G25210 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 2.4e-144
Identity = 253/538 (47.03%), Postives = 360/538 (66.91%), Query Frame = 0

Query: 13  LIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDY-GSSSTNPIANLIYTPTNSSYSSIL 72
           +I F +   + +S A P        E+F++C  +Y  ++  NP    IYT  +  Y S+L
Sbjct: 14  IIFFFLSFNIQISIANP-------QENFLKCFSEYIPNNPANP--KFIYTQHDQLYMSVL 73

Query: 73  NFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISD 132
           N +IQNLRF +   PKP  I+TP +VSHIQA+++CSK  G QIRTRSGGHD EGLSYIS 
Sbjct: 74  NSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQ 133

Query: 133 IPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGH 192
           +PF +VDL N+ ++ +D+ +Q AWV +GATLGE+YY I+E ++  +FP G CPTVGVGGH
Sbjct: 134 VPFAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGH 193

Query: 193 LSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAW 252
            SGGGYG L+R YGLAAD++IDA+LV+ +GKV +++SMGEDLFWAIRGGGG +FGI+ AW
Sbjct: 194 FSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAW 253

Query: 253 KVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE-E 312
           K+KLV VP++ TI    + +E   ++KL ++WQ +A K D +L+L        +T++  +
Sbjct: 254 KIKLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGK 313

Query: 313 GKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVL----LMGWFQKE 372
            K      FSS+FLG  + ++ ++  +FP+LG+ K DC E+SWI + +    ++ +    
Sbjct: 314 NKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTAN 373

Query: 373 DPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMS 432
              E+LL+RS        IK DYVK+ IP  A+  + E+L  ++V +   +  PYGG M 
Sbjct: 374 FKKEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMD 433

Query: 433 EVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYV 492
           E+ +S  PF HR+   Y + Y A W+    E  + H+NW+R +Y++ TP+VS +PR AY+
Sbjct: 434 EISESAIPFPHRAGIMYELWYTATWE--KQEDNEKHINWVRSVYNFTTPYVSQNPRLAYL 493

Query: 493 NYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 545
           NYRDLD+G  N     +Y +AR+WG KYFGKNF+RLV VKTK DP++FFR+EQSIP L
Sbjct: 494 NYRDLDLGKTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of CSPI01G25210 vs. ExPASy TrEMBL
Match: A0A5D3C112 (Cannabidiolic acid synthase-like 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00400 PE=3 SV=1)

HSP 1 Score: 1036.2 bits (2678), Expect = 5.1e-299
Identity = 514/556 (92.45%), Postives = 532/556 (95.68%), Query Frame = 0

Query: 1   MKCFPILPAPLGLIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIY 60
           MKCFPIL AP GLIAFII  VVVLSTAT SFC TQE +SFVQCLFDYGS ++NPIAN+IY
Sbjct: 1   MKCFPILAAPFGLIAFII--VVVLSTATTSFC-TQECDSFVQCLFDYGSPNSNPIANVIY 60

Query: 61  TPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120
           TP+NSSYSSILNFSIQNLRFLNK+I KP AIITP HVSHIQAAVICSKSHGFQIRTRSGG
Sbjct: 61  TPSNSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGG 120

Query: 121 HDFEGLSYISDIPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPA 180
           HDFEGLSYIS +PFV+VDLINL SISIDVENQNAWV SGATLGELYYRI+EKSQTLAFPA
Sbjct: 121 HDFEGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240
           GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG
Sbjct: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240

Query: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300
           GGG FGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL
Sbjct: 241 GGGCFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300

Query: 301 TGGKVTEDEEGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLM 360
           TGGKVTEDEEGKINPIASFS MFLGKAEEVLTILKP FPQL L KEDCLEMSWIQSVLLM
Sbjct: 301 TGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLM 360

Query: 361 GWFQKEDPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVP 420
           GWFQK DPLEVLLNR+RLYSEISKIKSDYVKE IP+VA+KGMWERLKS+DVELSQIMFVP
Sbjct: 361 GWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVP 420

Query: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNS 480
           YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAE+ HLNWIREIYDYMTPFVS S
Sbjct: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKS 480

Query: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS 540
           PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFD+LV+VKTKVDPSDFFRH+QS
Sbjct: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQS 540

Query: 541 IPTLSGISKELFSASL 557
           IPTLSGIS+E FSASL
Sbjct: 541 IPTLSGISEESFSASL 553

BLAST of CSPI01G25210 vs. ExPASy TrEMBL
Match: A0A1S3BEA9 (cannabidiolic acid synthase-like 2 OS=Cucumis melo OX=3656 GN=LOC103488685 PE=3 SV=1)

HSP 1 Score: 1036.2 bits (2678), Expect = 5.1e-299
Identity = 514/556 (92.45%), Postives = 532/556 (95.68%), Query Frame = 0

Query: 1   MKCFPILPAPLGLIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIY 60
           MKCFPIL AP GLIAFII  VVVLSTAT SFC TQE +SFVQCLFDYGS ++NPIAN+IY
Sbjct: 1   MKCFPILAAPFGLIAFII--VVVLSTATTSFC-TQECDSFVQCLFDYGSPNSNPIANVIY 60

Query: 61  TPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120
           TP+NSSYSSILNFSIQNLRFLNK+I KP AIITP HVSHIQAAVICSKSHGFQIRTRSGG
Sbjct: 61  TPSNSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGG 120

Query: 121 HDFEGLSYISDIPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPA 180
           HDFEGLSYIS +PFV+VDLINL SISIDVENQNAWV SGATLGELYYRI+EKSQTLAFPA
Sbjct: 121 HDFEGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240
           GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG
Sbjct: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240

Query: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300
           GGG FGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL
Sbjct: 241 GGGCFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300

Query: 301 TGGKVTEDEEGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLM 360
           TGGKVTEDEEGKINPIASFS MFLGKAEEVLTILKP FPQL L KEDCLEMSWIQSVLLM
Sbjct: 301 TGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLM 360

Query: 361 GWFQKEDPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVP 420
           GWFQK DPLEVLLNR+RLYSEISKIKSDYVKE IP+VA+KGMWERLKS+DVELSQIMFVP
Sbjct: 361 GWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVP 420

Query: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNS 480
           YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAE+ HLNWIREIYDYMTPFVS S
Sbjct: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKS 480

Query: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS 540
           PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFD+LV+VKTKVDPSDFFRH+QS
Sbjct: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQS 540

Query: 541 IPTLSGISKELFSASL 557
           IPTLSGIS+E FSASL
Sbjct: 541 IPTLSGISEESFSASL 553

BLAST of CSPI01G25210 vs. ExPASy TrEMBL
Match: A0A1S3BE36 (cannabidiolic acid synthase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488617 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.9e-213
Identity = 360/536 (67.16%), Postives = 436/536 (81.34%), Query Frame = 0

Query: 13  LIAFIIIVVVVLSTATPSFC-STQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSIL 72
           L+    +  +VL+  +PS+  ST++ E+F+QCLF+   ++   I+NLIYTP NSS+ S+L
Sbjct: 7   LLTPFALFFIVLTFLSPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPINSSFYSVL 66

Query: 73  NFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISD 132
           NFSIQNLRF  K  PKP AIITP HVSHIQAA+ICSKSH  QIR RSGGHDFEGLSY+SD
Sbjct: 67  NFSIQNLRFSRKQTPKPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDFEGLSYVSD 126

Query: 133 IPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGH 192
           +PF+VVDLINL+SI+IDVE +NAWV SGATLGE YYRI EKS+TLAFPAGSCPTVG+GGH
Sbjct: 127 VPFIVVDLINLRSITIDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSCPTVGIGGH 186

Query: 193 LSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAW 252
           LSGGG+GWL+RKYGLAAD+VIDA  +DANGKV+++ESMGEDLFWAIRGGGGGSFGI+VAW
Sbjct: 187 LSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGIIVAW 246

Query: 253 KVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEG 312
           KVKLV VPA VTIC + R+LEE+  +KLIH+WQY+   LD NLLLGISLTGG +T+ E G
Sbjct: 247 KVKLVRVPATVTICGSKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGGNLTQ-ESG 306

Query: 313 KINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWFQKEDPLEV 372
           K NP A FSS FLGK  E++ IL   FP+L L+KE+C EMSWI++VL+M  F   +P EV
Sbjct: 307 KTNPNALFSSFFLGKVNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGFPTHEPFEV 366

Query: 373 LLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDS 432
           LLNR+ L+   +KIKSDY+K+ +   A K M +RLK++D+E++QIMF+PYGG+MSE+  S
Sbjct: 367 LLNRTPLFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGGRMSEISKS 426

Query: 433 ETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYRDL 492
           ETPF HR    Y +GY  KWK+ S++AEK HL+WIR+IYDYMTPFVS SPRA Y NYRDL
Sbjct: 427 ETPFPHRVGNIYKLGYYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRATYSNYRDL 486

Query: 493 DIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLSGI 548
           DIG NNKYG+ +Y  ARVWG KYFGKNFDRLVHVKT +DP+DFFR+EQSIP L  I
Sbjct: 487 DIGINNKYGKATYSHARVWGFKYFGKNFDRLVHVKTMIDPNDFFRNEQSIPALIDI 541

BLAST of CSPI01G25210 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 2.0e-210
Identity = 360/537 (67.04%), Postives = 435/537 (81.01%), Query Frame = 0

Query: 10  PLGLIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTN-PIANLIYTPTNSSYS 69
           P  LI+  +  + + S++  +     + ESF+QCL D+    +N  I+ +I+TP NSSYS
Sbjct: 4   PSPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSYS 63

Query: 70  SILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSY 129
           S+L+FSI+NLRF   + PKP  I+TP HVSHIQAAVICSKS+GFQIRTRSGGHDFEGLSY
Sbjct: 64  SVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSY 123

Query: 130 ISDIPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGV 189
           ++ +PF++VDLINL+SISID+++  AWV SGATLGELYY I++KS+TLAFPAG CPTVGV
Sbjct: 124 VAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGV 183

Query: 190 GGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIV 249
           GGH SGGGYG L+RKYGLAAD+VIDAYLVDA+GK HN+ESMGEDLFWAIRGGGGGSFGIV
Sbjct: 184 GGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIV 243

Query: 250 VAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTED 309
           VAWKVKLV VPA VT+C ANRTLEE  + K+ H WQYVA KLD NL LGI   GGK+T  
Sbjct: 244 VAWKVKLVPVPATVTLCSANRTLEEGAV-KITHRWQYVANKLDENLFLGILWLGGKITSQ 303

Query: 310 EEGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMG-WFQKED 369
           E GK NP+A+F S+FLGKA+E+L+IL   FP+L LAK+DC+E SWI+S +LMG  F  E 
Sbjct: 304 EGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTEL 363

Query: 370 PLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSE 429
            LE LL+R+ L +  SKIKSDYVKE +    V+G+W+RL SQD+E   I+FVPYGG+MS+
Sbjct: 364 TLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQ 423

Query: 430 VDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVN 489
           + +SETPFSHR+ Y Y +GYIA W+   ++AEK HL WIRE+YDYMTPFVS SPRAAY N
Sbjct: 424 ISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYAN 483

Query: 490 YRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTL 545
           YRDLDIG+NNK+G+TSYK+A VWGLKYFG NF+RLVHVKTKVDP DFFRHEQSIP L
Sbjct: 484 YRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 539

BLAST of CSPI01G25210 vs. ExPASy TrEMBL
Match: A0A6J1DEU9 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020168 PE=3 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 7.5e-210
Identity = 358/545 (65.69%), Postives = 431/545 (79.08%), Query Frame = 0

Query: 1   MKCFPILPAPLGLIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIY 60
           MK FP+ P     +AF     +VLS++     S  + E F+QCL  + S S N I+  IY
Sbjct: 1   MKSFPLNP-----LAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRH-SPSNNSISKFIY 60

Query: 61  TPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120
           TP N SYSSILNFSIQNLRF   + PKP  +I P H+SHIQAAVICSK+HG QIRTRSGG
Sbjct: 61  TPANFSYSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGG 120

Query: 121 HDFEGLSYISDIPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPA 180
           HDFEGLSY+S +PF+V+DLI+L+SI+ID +N  AWV SGATLGELYYRI++KSQTLAFPA
Sbjct: 121 HDFEGLSYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240
           GSCPTVGVGGHLSGGGYG+LIRKYGLAADHVIDA+LVDANG++ N+ESM +DLFW IRGG
Sbjct: 181 GSCPTVGVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGG 240

Query: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300
           GGGSFGIVVAWK++LV VP  +T C  NRTL+ D + KLIH WQYVA KL+ NLLLGISL
Sbjct: 241 GGGSFGIVVAWKLRLVPVPTTLTTCTVNRTLDRDGV-KLIHRWQYVAHKLEDNLLLGISL 300

Query: 301 TGGKVTEDEEGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLM 360
           TGG +   + GK NP+ASF + FLGK +E++ I+   FP+LGL KEDC+E SWI+S L+ 
Sbjct: 301 TGGHILTQQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLIT 360

Query: 361 GWFQKEDPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVP 420
             F   + LE LL R  L +  +KIKSDYVKE I     KG+WERL +QD+EL+Q+MFVP
Sbjct: 361 AGFSAGESLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVP 420

Query: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNS 480
           YGG+MSE  +SETP+ HR++  Y +GYI KW+  S +AEK HLNW+REIYDYM P+V+ S
Sbjct: 421 YGGRMSEFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKS 480

Query: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS 540
           PRAAYVNYRDLDIG NN+Y +T+Y+RA+VWGLKYFGKNF+RLV VKT+VDPSDFFRHEQS
Sbjct: 481 PRAAYVNYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQS 538

Query: 541 IPTLS 546
           IPT S
Sbjct: 541 IPTFS 538

BLAST of CSPI01G25210 vs. NCBI nr
Match: XP_004135742.2 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653322.1 hypothetical protein Csa_023372 [Cucumis sativus])

HSP 1 Score: 1109.0 bits (2867), Expect = 0.0e+00
Identity = 548/556 (98.56%), Postives = 550/556 (98.92%), Query Frame = 0

Query: 1   MKCFPILPAPLGLIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIY 60
           MKCFPILPAP GLIAFIII+VVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIY
Sbjct: 1   MKCFPILPAPFGLIAFIIIIVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIY 60

Query: 61  TPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120
           TPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGG
Sbjct: 61  TPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120

Query: 121 HDFEGLSYISDIPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPA 180
           HDFEGLSYISDIPFV+VDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPA
Sbjct: 121 HDFEGLSYISDIPFVLVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240
           GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG
Sbjct: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240

Query: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300
           GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL
Sbjct: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300

Query: 301 TGGKVTEDEEGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLM 360
           TGGKVTEDEEGKINPIASFS MFLGKAEEVLTILKP FPQLGL KEDCLEMSWIQSVLLM
Sbjct: 301 TGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLM 360

Query: 361 GWFQKEDPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVP 420
           GWFQKEDPLEVLLNRSRLYSEISKIKSDYVKE IPMVAVKGMWERLKSQDVELSQIMFVP
Sbjct: 361 GWFQKEDPLEVLLNRSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVP 420

Query: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNS 480
           YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVS S
Sbjct: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKS 480

Query: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS 540
           PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS
Sbjct: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS 540

Query: 541 IPTLSGISKELFSASL 557
           IPTLSGISKELFSASL
Sbjct: 541 IPTLSGISKELFSASL 556

BLAST of CSPI01G25210 vs. NCBI nr
Match: XP_008445757.1 (PREDICTED: cannabidiolic acid synthase-like 2 [Cucumis melo] >KAA0047085.1 cannabidiolic acid synthase-like 2 [Cucumis melo var. makuwa] >TYK05070.1 cannabidiolic acid synthase-like 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1036.2 bits (2678), Expect = 1.0e-298
Identity = 514/556 (92.45%), Postives = 532/556 (95.68%), Query Frame = 0

Query: 1   MKCFPILPAPLGLIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIY 60
           MKCFPIL AP GLIAFII  VVVLSTAT SFC TQE +SFVQCLFDYGS ++NPIAN+IY
Sbjct: 1   MKCFPILAAPFGLIAFII--VVVLSTATTSFC-TQECDSFVQCLFDYGSPNSNPIANVIY 60

Query: 61  TPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120
           TP+NSSYSSILNFSIQNLRFLNK+I KP AIITP HVSHIQAAVICSKSHGFQIRTRSGG
Sbjct: 61  TPSNSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGG 120

Query: 121 HDFEGLSYISDIPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPA 180
           HDFEGLSYIS +PFV+VDLINL SISIDVENQNAWV SGATLGELYYRI+EKSQTLAFPA
Sbjct: 121 HDFEGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240
           GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG
Sbjct: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240

Query: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300
           GGG FGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL
Sbjct: 241 GGGCFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300

Query: 301 TGGKVTEDEEGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLM 360
           TGGKVTEDEEGKINPIASFS MFLGKAEEVLTILKP FPQL L KEDCLEMSWIQSVLLM
Sbjct: 301 TGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLM 360

Query: 361 GWFQKEDPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVP 420
           GWFQK DPLEVLLNR+RLYSEISKIKSDYVKE IP+VA+KGMWERLKS+DVELSQIMFVP
Sbjct: 361 GWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVP 420

Query: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNS 480
           YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAE+ HLNWIREIYDYMTPFVS S
Sbjct: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKS 480

Query: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS 540
           PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFD+LV+VKTKVDPSDFFRH+QS
Sbjct: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQS 540

Query: 541 IPTLSGISKELFSASL 557
           IPTLSGIS+E FSASL
Sbjct: 541 IPTLSGISEESFSASL 553

BLAST of CSPI01G25210 vs. NCBI nr
Match: XP_038895846.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 958.7 bits (2477), Expect = 2.1e-275
Identity = 465/554 (83.94%), Postives = 513/554 (92.60%), Query Frame = 0

Query: 1   MKCFPILPAPLGLIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIY 60
           M+CFP L APLG IAFII   +VL+T T SFC TQES+SFVQCLFDYGS +T+PI+++IY
Sbjct: 1   MECFP-LTAPLGRIAFIIF--IVLTTTTTSFC-TQESDSFVQCLFDYGSLNTHPISDVIY 60

Query: 61  TPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120
           TP NSSYSS+LNFS+QNLRFL K  PKP AIITP HVSHIQAAVICSKSHG QIRTRSGG
Sbjct: 61  TPINSSYSSVLNFSVQNLRFLTKKTPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGG 120

Query: 121 HDFEGLSYISDIPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPA 180
           HDFEGLSYISD PF+VVDLINL+SI+IDVEN+NAWV SGATLGELYYRI+EKSQTLAFPA
Sbjct: 121 HDFEGLSYISDAPFIVVDLINLRSIAIDVENENAWVQSGATLGELYYRIAEKSQTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240
           GSC TVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGK+H++ESMGEDLFWAIRGG
Sbjct: 181 GSCSTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKLHDRESMGEDLFWAIRGG 240

Query: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300
           GGGSFGIVV+WKVKLV VPA VT+CEANRTLEED ++KLIHEWQYVAPKLDANLLLGISL
Sbjct: 241 GGGSFGIVVSWKVKLVRVPATVTVCEANRTLEED-VMKLIHEWQYVAPKLDANLLLGISL 300

Query: 301 TGGKVTEDEEGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLM 360
           TGGKVTEDEEGKINPIASF + FLGKAEE++TILKP F QLGL +EDCLE+SWI+SVLLM
Sbjct: 301 TGGKVTEDEEGKINPIASFVAQFLGKAEELMTILKPTFSQLGLKREDCLEISWIESVLLM 360

Query: 361 GWFQKEDPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVP 420
           GWFQKEDP+EVLLNR+RLYSEISKIKSDYVKERIP  ++KGMWERLKSQDVEL+QIMFVP
Sbjct: 361 GWFQKEDPIEVLLNRTRLYSEISKIKSDYVKERIPKASIKGMWERLKSQDVELAQIMFVP 420

Query: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNS 480
           YGG+MSEV + ETP+SHR DY YL+GYIAKWKNG+VEAEK HLNWIREIYDYMTPFVSNS
Sbjct: 421 YGGRMSEVYEWETPYSHRGDYMYLIGYIAKWKNGTVEAEKRHLNWIREIYDYMTPFVSNS 480

Query: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS 540
           PR AYVNYRDLDIGTNNKY +TSYKRAR WG+KYFG NFDRLV++KTKVDPS+FF+HEQS
Sbjct: 481 PRVAYVNYRDLDIGTNNKYDKTSYKRARNWGMKYFGNNFDRLVYIKTKVDPSNFFKHEQS 540

Query: 541 IPTLSGISKELFSA 555
           IP L+GI++E FSA
Sbjct: 541 IPILTGITEEFFSA 549

BLAST of CSPI01G25210 vs. NCBI nr
Match: XP_038895208.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 779.6 bits (2012), Expect = 1.8e-221
Identity = 391/549 (71.22%), Postives = 453/549 (82.51%), Query Frame = 0

Query: 1   MKCFPILPAPLGLIAFIIIVVVVLSTATPSFC--STQESESFVQCLFDYGSSSTNPIANL 60
           MK F + P  L    FI++ +      +PS+   ST++ E+FVQCL +  S ST PI+NL
Sbjct: 1   MKSFLLTPFAL---TFIVLSI------SPSWVVFSTKKHENFVQCLLN-NSLSTYPISNL 60

Query: 61  IYTPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRS 120
           IYTP NSSYSSILNFSIQNLRF +K+ PKP AIITP HVSHIQAA+ICSKSH  QIRTRS
Sbjct: 61  IYTPINSSYSSILNFSIQNLRFSSKETPKPLAIITPSHVSHIQAAIICSKSHALQIRTRS 120

Query: 121 GGHDFEGLSYISDIPFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAF 180
           GGHDFEGLSYISD+PFVVVDLINLKSI+IDVEN+ AWV SGATLGELYYRI+EKS+TLAF
Sbjct: 121 GGHDFEGLSYISDVPFVVVDLINLKSIAIDVENETAWVQSGATLGELYYRIAEKSRTLAF 180

Query: 181 PAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIR 240
           PAGSCPTVGVGGHLSGGG+GWL+RKYGLAAD+VIDA  +DANGKV+++ESMGEDLFWAIR
Sbjct: 181 PAGSCPTVGVGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIR 240

Query: 241 GGGGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGI 300
           GGGGGSFGIVVAWKVKLV VPA VTIC  NR LEE+  +KLIH WQYV  KLD NLLLGI
Sbjct: 241 GGGGGSFGIVVAWKVKLVRVPATVTICGINRNLEEEDTIKLIHRWQYVTNKLDENLLLGI 300

Query: 301 SLTGGKVTEDEEGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVL 360
           SL GG  T+ E GK NP+A FSS FLGK  E++TIL   F +LGLAKEDC EMSWI+SVL
Sbjct: 301 SLIGGNSTQ-EGGKTNPMALFSSFFLGKVNELVTILNTNFSELGLAKEDCSEMSWIKSVL 360

Query: 361 LMGWFQKEDPLEVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMF 420
           +M  F K++ LEVLLNR+      +KIKSDYVKE I   + K M++RLK++D+E++QIMF
Sbjct: 361 IMAGFPKQEALEVLLNRAPPSGLSTKIKSDYVKEPISKASFKTMFKRLKAEDIEVAQIMF 420

Query: 421 VPYGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVS 480
           +PYGG+MSE+ +S TPF HR+   Y +GY  KWK  S++AEK HLNW+R IYDYMTPFVS
Sbjct: 421 IPYGGRMSEISESLTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWVRVIYDYMTPFVS 480

Query: 481 NSPRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHE 540
            SPRAAY NYRDLDIGTNNK G+TSY RARVWGLKYFGKNFDRLVHVKTK+DP++FFR+E
Sbjct: 481 KSPRAAYNNYRDLDIGTNNKCGKTSYNRARVWGLKYFGKNFDRLVHVKTKIDPTNFFRNE 538

Query: 541 QSIPTLSGI 548
           QSIPTL+ I
Sbjct: 541 QSIPTLTDI 538

BLAST of CSPI01G25210 vs. NCBI nr
Match: XP_011648520.1 (cannabidiolic acid synthase [Cucumis sativus] >KAE8653301.1 hypothetical protein Csa_023300 [Cucumis sativus])

HSP 1 Score: 760.0 bits (1961), Expect = 1.4e-215
Identity = 360/527 (68.31%), Postives = 433/527 (82.16%), Query Frame = 0

Query: 22  VVLSTATPSFC-STQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQNLRF 81
           +VLS  +PS+  ST+  E+F+QCL +   ++   I+NLIYTP NSS+ S+LNFSIQNLRF
Sbjct: 14  IVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQNLRF 73

Query: 82  LNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVVVDLI 141
             K  PKP AIITP HVSHIQA ++CSKSH  QIR RSGGHDFEGLSY+SD+PF++VDLI
Sbjct: 74  SRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIVDLI 133

Query: 142 NLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGYGWL 201
           NL+SI+IDVEN+NAWV SGATLGE YYRI EKSQTLAFPAGSCPTVG+GGHLSGGG+GWL
Sbjct: 134 NLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGFGWL 193

Query: 202 IRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVEVPA 261
           +RKYGLAAD+VIDA  VDANGKV+++ESMG+DLFWAIRGGGGGSFGI+VAWKVKLV VPA
Sbjct: 194 MRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVRVPA 253

Query: 262 RVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFS 321
            VTIC + R+LEE+  +KLIH+WQY+  KLD NLLLGISLTGG  T+ E GKINP A FS
Sbjct: 254 TVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ-ESGKINPTALFS 313

Query: 322 SMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSRLYS 381
           S FLGK  E++ IL   FP+L L+KE+C EMSWI++VL M  F  ++P EVLLNR+  + 
Sbjct: 314 SFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPFEVLLNRTPPFG 373

Query: 382 EISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSHRSD 441
             +KIKSDY+K+ +   A K M +RLK+QD+E++QIMF+PYGG+MSE+ +SE PF HR+ 
Sbjct: 374 LSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIPFPHRAG 433

Query: 442 YSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYRDLDIGTNNKYG 501
             Y +GY  KWK+ S++AEK HLNWIR+IYDYMTPFVS SPRA Y NYRDLDIG NNKYG
Sbjct: 434 NIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNYRDLDIGMNNKYG 493

Query: 502 RTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLSGI 548
           + +Y  ARVWG KYFGKNFDRLVH+KTK+DP+DFFR+EQSIP L  I
Sbjct: 494 KATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNI 539

BLAST of CSPI01G25210 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 515.8 bits (1327), Expect = 4.4e-146
Identity = 253/532 (47.56%), Postives = 359/532 (67.48%), Query Frame = 0

Query: 13  LIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILN 72
           ++ F     ++LS   P   +     SF+QCL     + +N ++ +I+TP ++S+SS+L 
Sbjct: 10  VLIFFTTSTLLLSIPHPVSANRSNQSSFLQCL-SLQLNDSNIVSKVIHTPNDTSFSSVLA 69

Query: 73  FSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDI 132
            SIQN RF   D+PKP  I+TP+  S +Q+AV C++  G  IRTRSGGHD+EGLSY++  
Sbjct: 70  SSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHK 129

Query: 133 PFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHL 192
           PFV++DL NL+SI++DV+N++ WV +GAT+GELYY I +K++TLAFPAG CPTVGVGGH 
Sbjct: 130 PFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHF 189

Query: 193 SGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWK 252
           SGGGYG L+RK+GLAADHVIDA +VDA G++  +  MGED FWAIRGGGG SF +V++WK
Sbjct: 190 SGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWK 249

Query: 253 VKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGK 312
           + L+ VP+ VT+    +   E   LK+IH WQ+VA K+  +L + + L        +  K
Sbjct: 250 IGLINVPSTVTVFNVTK-FSEQSALKIIHRWQFVADKVSDDLFIRVML--------QRYK 309

Query: 313 INPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWFQK--EDPLE 372
               ASF  ++LG  + +L ++   FP+LGL ++DC EMSWI+SV+   WF +  E+P+ 
Sbjct: 310 NMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVI---WFAELGEEPIN 369

Query: 373 VLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDD 432
           VL  R+R  S   K KSD+V+E +P  A+  +W RL+  + E +Q++F P+GGKMSE+ D
Sbjct: 370 VLTKRTRA-SLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIAD 429

Query: 433 SETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYRD 492
            ETPF HR    Y + Y+  W+    + ++ ++ W+  +YD M+ FV+ SPR AY+N RD
Sbjct: 430 YETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRD 489

Query: 493 LDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIP 543
           LD+G      R+ Y+  + WG+KYF  NF+RLV VKT VDPSDFF  EQSIP
Sbjct: 490 LDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of CSPI01G25210 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 501.9 bits (1291), Expect = 6.6e-142
Identity = 253/534 (47.38%), Postives = 365/534 (68.35%), Query Frame = 0

Query: 15  AFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTP--TNSSYSSILN 74
           AF++ ++++  +  P   ST + + FV CL D  S ++ P+ +  +T     SS+   L 
Sbjct: 11  AFLVTLLLLSLSYIPLSFSTIQ-QDFVMCLVD-NSDASFPMDSSFFTHDLNASSFKLALE 70

Query: 75  FSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDI 134
            S QNLR+L    PKP  I  PL+ +H+QAAV+C+K     +R RSGGHD+EGLSY+S++
Sbjct: 71  TSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEM 130

Query: 135 --PFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGG 194
              FV+VDL  L+ IS+D+E+ +AWVH+GA++GE+YYRI EKS+   FPAG C ++G+GG
Sbjct: 131 ETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGG 190

Query: 195 HLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVA 254
           H+ GG YG ++RK+GL AD+V+DA +VDA+GK+ N+ +MGED+FWAIRGGGGGSFG+++A
Sbjct: 191 HIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILA 250

Query: 255 WKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL-TGGKVTEDE 314
           WK+KLV VP  VT+    RTLE+D   KL+++WQ VA KLD +L + + +    K  + +
Sbjct: 251 WKIKLVPVPEIVTVFTVTRTLEQDG-TKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSK 310

Query: 315 EGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWFQKEDPL 374
           E  I+   S+   FLG A  +L +++ +FPQLGL K+DCLE SWI+SV+ +  F    P 
Sbjct: 311 ERTIS--TSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPS 370

Query: 375 EVLLNRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVD 434
           E LL+   L+    K KSDYV+E IP+  ++G+WE+L  +D  L+  ++ PYGG M+++ 
Sbjct: 371 EALLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLT--IWNPYGGMMAKIP 430

Query: 435 DSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYR 494
           ++ETPF HRS   + + ++  W++G   +E  H+ W+RE+Y YM  +VS SPR+AYVNYR
Sbjct: 431 ETETPFPHRSGTLFKIQWLTLWQDGKT-SEAKHMGWMREMYSYMEQYVSKSPRSAYVNYR 490

Query: 495 DLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPT 544
           DLD+G N K        AR WG +YF  NF+RLV +K K DP +FFRHEQSIPT
Sbjct: 491 DLDLGMNGK-----GSDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPT 531

BLAST of CSPI01G25210 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 500.7 bits (1288), Expect = 1.5e-141
Identity = 253/543 (46.59%), Postives = 360/543 (66.30%), Query Frame = 0

Query: 10  PLGLIAFIIIVVVVLS--TATPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTN--S 69
           PL  I+ II+ V+  S    TP+  S    + F+ C+    +  + P+   ++TP    S
Sbjct: 6   PLPTISCIIVSVLYFSFYCITPTSSSASIQDQFINCV-KRNTHVSFPLEKTLFTPAKNVS 65

Query: 70  SYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEG 129
            ++ +L  + QNL+FL K +PKP  I  P+H S +QA++ICSK  G   R RSGGHDFE 
Sbjct: 66  LFNQVLESTAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEA 125

Query: 130 LSYISDI--PFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSC 189
           LSY+S I  PF+++DL  LK I++D+E+ +AWV  GATLGELYYRI+EKS+   FPAG C
Sbjct: 126 LSYVSRIEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLC 185

Query: 190 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 249
            +VG+GG+++GGGYG L+RKYGLA D+V+D  +VDANGK+ ++ +MGEDLFWAIRGGGG 
Sbjct: 186 TSVGIGGYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGA 245

Query: 250 SFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 309
           SFGIV+AWK+KLV VP  VT+    +TLE+D  LK I +WQ ++ K+   + + + L   
Sbjct: 246 SFGIVLAWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAA 305

Query: 310 KVTEDEEGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWF 369
                 +G      ++   FLG+   +L +++ AFP+LGL ++DC EMSWI++ L  G F
Sbjct: 306 ----GNDGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGF 365

Query: 370 QKEDPLEVLLN-RSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYG 429
               P+E+LL  +S L  +  K  SD+VKE IP++ +KG+++RL   +     + + PYG
Sbjct: 366 PTGSPIEILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTF--LNWTPYG 425

Query: 430 GKMSEVDDSETPFSHRSDYSYLVGYIAKW-KNGSVEAEKSHLNWIREIYDYMTPFVSNSP 489
           G MS++ +S  PF HR+   + + Y A W +N    + K  +NWI+EIY+YM P+VS++P
Sbjct: 426 GMMSKIPESAIPFPHRNGTLFKILYYANWLENDKTSSRK--INWIKEIYNYMAPYVSSNP 485

Query: 490 RAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSI 545
           R AYVNYRDLD G N    + ++  A++WG KYF  NFDRLV +KTKVDP +FFRHEQSI
Sbjct: 486 RQAYVNYRDLDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSI 539

BLAST of CSPI01G25210 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 498.0 bits (1281), Expect = 9.5e-141
Identity = 247/544 (45.40%), Postives = 365/544 (67.10%), Query Frame = 0

Query: 10  PLGLIAFIIIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTN--SSY 69
           PL L + I+++   L T TP+       + F+ C+    +    P+    + PT   S +
Sbjct: 6   PLPLFS-ILVLYFSLYTITPTSSLASLQDQFINCV-QRNTHVYFPLEKTFFAPTKNVSMF 65

Query: 70  SSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLS 129
           S +L  + QNLRFL K +PKP  I +P+H SH+QA++ICSK     +R RSGGHD+EGLS
Sbjct: 66  SQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLS 125

Query: 130 YISDI--PFVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPT 189
           Y+S I  PF+++DL  ++ ++I++++ +AWV SGAT+GELYYRI+EKS+   FPAG C +
Sbjct: 126 YVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSS 185

Query: 190 VGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSF 249
           +G+GGH++GG YG ++RKYGL AD+V+DA +VDANGK+ ++ +MGED FWAIRGG GGSF
Sbjct: 186 LGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSF 245

Query: 250 GIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANL----LLGISLT 309
           GI++AWK+KLV VP  VT+    +TL++D   K+I +WQ VA KL   L    L  ++ T
Sbjct: 246 GIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGT 305

Query: 310 GGKVTEDEEGKINPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMG 369
           GG  T           S++++FLG    ++ ++K +FP+LGL  +DC+EMSW++S+  + 
Sbjct: 306 GGNKT--------VTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYIS 365

Query: 370 WFQKEDPLEVLLNRSRLYSEIS-KIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVP 429
            F    P  VLL     + ++S K KSD+VK  IP   ++G++++L  +D+ L  +++ P
Sbjct: 366 GFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNP 425

Query: 430 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNS 489
           YGG M+++ +S+ PF HR    + V Y+  W + S +    H+NWIR++Y YMTP+VS++
Sbjct: 426 YGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSN 485

Query: 490 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS 545
           PR AYVNYRDLD+G N K  +T  K+A+VWG  YF  NF+RL+ +K KVDP +FFRHEQS
Sbjct: 486 PREAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQS 536

BLAST of CSPI01G25210 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 496.9 bits (1278), Expect = 2.1e-140
Identity = 244/533 (45.78%), Postives = 361/533 (67.73%), Query Frame = 0

Query: 18  IIVVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIYTPTNSS--YSSILNFSI 77
           + +V +L  + P   ST + + FV+CL D  S  + PI    ++P  ++  +   L  + 
Sbjct: 12  LFLVTLLLISVPLSSSTLQ-QDFVKCLVD-NSDVSFPITASFFSPDQNATLFKEELESTA 71

Query: 78  QNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYIS--DIP 137
           QNLR+L    PKP  I  PL+ +H+QAAV+C+K     +R RSGGHD+EGLS+++  + P
Sbjct: 72  QNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETP 131

Query: 138 FVVVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLS 197
           FV+VDL  L+ + +D+++ +AW H+GAT+GE+YYRI EKSQT  FPAG C ++G+GGHL 
Sbjct: 132 FVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLV 191

Query: 198 GGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKV 257
           GG YG ++RK+GL AD+V+DA +VDANG++ ++ +MGED+FWAIRGGGGGSFG+++AWK+
Sbjct: 192 GGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKI 251

Query: 258 KLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKI 317
           KLV VPA VT+    +TLE+D   K++++W+ +A KLD +L + + ++    T  + G  
Sbjct: 252 KLVPVPATVTVFTVTKTLEQDG-TKVLYKWEQIADKLDDDLFIRVIISPASKT-TKPGNR 311

Query: 318 NPIASFSSMFLGKAEEVLTILKPAFPQLGLAKEDCLEMSWIQSVLLMGWFQKEDPLEVLL 377
               S+ + FLG +  +L +++ +FP+LGL K+DC EMSWI+SV+ +  F      E LL
Sbjct: 312 TISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALL 371

Query: 378 NRSRLYSEISKIKSDYVKERIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSET 437
               L+    K KSD+VKE IP+  ++G+WER   +D  L+  ++ PYGG MS + +SE 
Sbjct: 372 AGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYGGMMSRISESEI 431

Query: 438 PFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSNSPRAAYVNYRDLDI 497
           PF HR+   + + +++ W++G V +E+ H+ WIRE+Y YM  +VS +PR AYVNYRDLD+
Sbjct: 432 PFPHRNGTLFKIQWLSTWQDGKV-SEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDL 491

Query: 498 GTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLSG 547
           GTN   G T    AR WG KY+  NF+RLV +K + DP +FFRHEQS+PT  G
Sbjct: 492 GTNE--GETD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTKIG 532