CSPI01G25200 (gene) Cucumber (PI 183967) v1

BLAST of CSPI01G25200 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 6.2e-142
Identity = 258/516 (50.00%), Postives = 343/516 (66.47%), Query Frame = 0

Query: 33  KDFLQCLSIHSTHIP--------IYTPINSSYSSILRSYAQNPRFSTQYNTLKPLVIIKP 92
           ++FL+C    S HIP        +YT  +  Y SIL S  QN RF +   T KPLVI+ P
Sbjct: 32  ENFLKCF---SKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISD-TTPKPLVIVTP 91

Query: 93  SHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFVVVDLINLKSIEVDTTNNTA 152
           S+ SH+Q+T++CSK  GLQIR RSGGHD EG+SYIS +PFVVVDL N+ SI++D  + TA
Sbjct: 92  SNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTA 151

Query: 153 WVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDA 212
           WV++GAT+GE+YY I EK+  L+FP G CPTVG+GGHFSGGGYG LMR YGLAADN+IDA
Sbjct: 152 WVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDA 211

Query: 213 YLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLD-K 272
           +LV+ +G V DR++MGE LFWAIRGGGG +FGI+  WK+KLV VP   T   + K+++  
Sbjct: 212 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIH 271

Query: 273 NTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPT---ASFFSLYLGKTDKLVTI 332
              K+  +WQ +A + ++ L++  +    N T    +N T     F S++ G  D LV +
Sbjct: 272 GLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDL 331

Query: 333 MNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----EILLSKPTLSNNISYKIKSD 392
           MN + P LG+ K +CKE SWI +T+  +G  N        EILL + +     ++ IK D
Sbjct: 332 MNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDR-SAGKKTAFSIKLD 391

Query: 393 YVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTPFSHRAEFLYKISYT 452
           YVK+PI + A   I ++L  ++V      L+PYGG M  IS S  PF HRA  +Y++ YT
Sbjct: 392 YVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYT 451

Query: 453 VGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIGTNNKYGKTSYEEAS 512
             W +Q    NE+H+NW+R  Y+F TP+VS +PR AY+NYRDLD+G  N     +Y +A 
Sbjct: 452 ASWEKQED--NEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQAR 511

Query: 513 IWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
           IWG KYFG NF RLV+VKT VDP+NFFR+EQSIPPL
Sbjct: 512 IWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of CSPI01G25200 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 5.2e-141
Identity = 262/530 (49.43%), Postives = 352/530 (66.42%), Query Frame = 0

Query: 16  IVSLFSTQWSTASSYKSKDFLQCLSIH----STHIP-IYTPINSSYSSILRSYAQNPRFS 75
           I   FS    T+ +   ++FL+C S +    +T++  +YT  N  Y S+L S   N RF 
Sbjct: 15  IFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRF- 74

Query: 76  TQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFVVVDLI 135
           T   T KPLVI+ PSHVSH+Q T++CSK  GLQIR RSGGHD+EG+SYIS +PFV+VDL 
Sbjct: 75  TSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLR 134

Query: 136 NLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGYGWL 195
           N++SI++D  + TAWV++GAT+GE+YY + EK+  L+  AG CPTV  GGHF GGGYG L
Sbjct: 135 NMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPL 194

Query: 196 MRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPP 255
           MR YGLAADN+IDA+LV+ +G V DR++MGE LFWA+RGGG  SFGI+V WK++LV VP 
Sbjct: 195 MRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPK 254

Query: 256 IVTTCRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPTA---SF 315
             T   + K ++     K+V +WQ +A + ++ LL+  +    N T    +N TA    F
Sbjct: 255 -STMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYF 314

Query: 316 FSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQP----LEILLSK 375
            S++LG  D LV +MN + P LG+ K +C++ SWI + +  +G  N        EILL +
Sbjct: 315 SSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDR 374

Query: 376 PTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTP 435
            +   N ++KIK DYVK+PI +  F  I ++L  +++      L+PYGG M  IS S  P
Sbjct: 375 -SAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIP 434

Query: 436 FSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIG 495
           F HRA  LY++ Y   W +Q    NE+HLNWIR  Y+FMTP+VS +PR AY+NYRDLDIG
Sbjct: 435 FPHRAGILYELWYICSWEKQED--NEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIG 494

Query: 496 TNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
            N+     +Y +A IWG KYFG NF RLV+VKT VDP+NFFR+EQSIPPL
Sbjct: 495 INDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of CSPI01G25200 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 3.4e-140
Identity = 261/528 (49.43%), Postives = 348/528 (65.91%), Query Frame = 0

Query: 20  FSTQWSTASSYKSKDFLQCLSIH-STHIP----IYTPINSSYSSILRSYAQNPRFSTQYN 79
           F+ Q S A+    ++FL+C S +  T++     +YT  +  Y SIL S  QN RF T   
Sbjct: 21  FNIQISIAN--PQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRF-TSDT 80

Query: 80  TLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFVVVDLINLKS 139
           T KPLVII P +VSH+Q T++CSK  GLQIR RSGGHD EG+SYIS +PFV+VDL N+ S
Sbjct: 81  TPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHS 140

Query: 140 IEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGYGWLMRKY 199
           +++D  + TAWV++GAT+GE+YY I E +  L+FPAG CPTVG GGHFSGGGYG LMR Y
Sbjct: 141 VKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNY 200

Query: 200 GLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPPIVTT 259
           GLAADN+IDA+LV+ +G V DR++MGE LFWAIRGGGG +FGI+  WK++LV VP + T 
Sbjct: 201 GLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTI 260

Query: 260 CRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPT---ASFFSLY 319
             + K+++     K+V +WQ +A   E++LL+  +    N T    +N T   + F S++
Sbjct: 261 FSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIF 320

Query: 320 LGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----EILLSKPTLS 379
            G  D LV +MN + P LG+ K +CK+ SWI + +  +G  N        EILL + +  
Sbjct: 321 HGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDR-SGG 380

Query: 380 NNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTPFSHR 439
              ++ IK DYVK+PI + A   I ++L  ++V       +PYGG M  IS S  PF HR
Sbjct: 381 RKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHR 440

Query: 440 AEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIGTNNK 499
           A  +Y+I Y   W +Q    NE+H+NWIR  Y+F TP+VS +PR AY+NYRDLD+G  N 
Sbjct: 441 AGIMYEIWYIASWEKQED--NEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNF 500

Query: 500 YGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTL 535
               +Y +A IWG KYFG NF RLV+VKT VDP NFFR+EQSIPPL L
Sbjct: 501 ESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPL 542

BLAST of CSPI01G25200 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 3.4e-140
Identity = 262/528 (49.62%), Postives = 347/528 (65.72%), Query Frame = 0

Query: 20  FSTQWSTASSYKSKDFLQCLSIH-STHIP----IYTPINSSYSSILRSYAQNPRFSTQYN 79
           F+ Q S A+    ++FL+CLS +  T++     +YT  +  Y SIL S  QN RF T   
Sbjct: 21  FNIQISIAN--PQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRF-TSDT 80

Query: 80  TLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFVVVDLINLKS 139
           T KPLVI  P +VSH+Q T++CSK  GLQIR RSGGHD EG+SYIS +PFV+VDL N+ S
Sbjct: 81  TPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHS 140

Query: 140 IEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGYGWLMRKY 199
           +++D  + TAWV+SGAT+GE+YY I E +  L+FPAG CPTVG GGHFSGGGYG LMR Y
Sbjct: 141 VKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNY 200

Query: 200 GLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPPIVTT 259
           GLAADN+IDA+LV+ +G V DR++MGE LFWAIRGGGG +FGI+  WK++LV VP + T 
Sbjct: 201 GLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTI 260

Query: 260 CRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPT---ASFFSLY 319
             + K+++     K+V +WQ +A   E++LL+  +    N T    +N T   + F S++
Sbjct: 261 FSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIF 320

Query: 320 LGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----EILLSKPTLS 379
            G  D LV +MN + P LG+ K +CK+ SWI + +  +G  N        EILL + +  
Sbjct: 321 HGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDR-SGG 380

Query: 380 NNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTPFSHR 439
              ++ IK DYVK+PI + A   I ++L  ++V       +PYGG M  IS S  PF HR
Sbjct: 381 RKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHR 440

Query: 440 AEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIGTNNK 499
           A   Y+I Y   W +Q    NE+H+NWIR  Y+F TP+VS +PR AY+NYRDLD+G  N 
Sbjct: 441 AGITYEIWYIASWEKQED--NEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNF 500

Query: 500 YGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTL 535
               +Y +A IWG KYFG NF RLV+VKT VDP NFFR+EQSIPPL L
Sbjct: 501 ESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPL 542

BLAST of CSPI01G25200 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 4.4e-140
Identity = 257/526 (48.86%), Postives = 346/526 (65.78%), Query Frame = 0

Query: 20  FSTQWSTASSYKSKDFLQCLSIHSTHIP-----IYTPINSSYSSILRSYAQNPRFSTQYN 79
           F+ Q S A+    ++FL+C S +  + P     IYT  +  Y S+L S  QN RF T   
Sbjct: 21  FNIQISIAN--PQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRF-TSDT 80

Query: 80  TLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFVVVDLINLKS 139
           T KPLVI+ PS+VSH+Q++++CSK  GLQIR RSGGHD EGLSYIS +PF +VDL N+ +
Sbjct: 81  TPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHT 140

Query: 140 IEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGYGWLMRKY 199
           ++VD  + TAWV++GAT+GE+YY I E +   +FP G CPTVG+GGHFSGGGYG LMR Y
Sbjct: 141 VKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNY 200

Query: 200 GLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPPIVTT 259
           GLAADN+IDA+LV+ +G V DR++MGE LFWAIRGGGG +FGI+  WK+KLV VP   T 
Sbjct: 201 GLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATI 260

Query: 260 CRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPT---ASFFSLY 319
             + K+++     K+  +WQ +A + ++ L++  +    N T    +N T     F S++
Sbjct: 261 FSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIF 320

Query: 320 LGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----EILLSKPTLS 379
           LG  D LV +MN + P LG+ K +CKE SWI +T+  +G  N        EILL + +  
Sbjct: 321 LGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDR-SAG 380

Query: 380 NNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTPFSHR 439
              ++ IK DYVK+ I + A   I ++L  +EV      L+PYGG M  IS S  PF HR
Sbjct: 381 KKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHR 440

Query: 440 AEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIGTNNK 499
           A  +Y++ YT  W +Q    NE+H+NW+R  Y+F TP+VS +PR AY+NYRDLD+G  N 
Sbjct: 441 AGIMYELWYTATWEKQED--NEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNP 500

Query: 500 YGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
               +Y +A IWG KYFG NF RLV+VKT  DP+NFFR+EQSIPPL
Sbjct: 501 ESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of CSPI01G25200 vs. ExPASy TrEMBL
Match: A0A5A7TYM8 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00390 PE=3 SV=1)

HSP 1 Score: 1021.5 bits (2640), Expect = 1.2e-294
Identity = 497/535 (92.90%), Postives = 513/535 (95.89%), Query Frame = 0

Query: 1   MKFFRLNPLFLISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHIPIYTPINSSYSSIL 60
           MK F LNPL   SFT+VSL STQWSTASSYK+KDFL+CLSIHST IPIYTPINSSY+S L
Sbjct: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60

Query: 61  RSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120
           RSYAQNPRFSTQY TLKPL IIKPSHVSH+QSTVICSKSHGLQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 180
           DLPF+VVDLINLKSIEVDTTNNT WVQSGATIGELYYRIAEKSRTLAFPAGVCPTVG+GG
Sbjct: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180

Query: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVG 240
            FSGGGYGWLMRKYGLAADNVIDAYLVDANG VFD EAMGEGLFWAIRGGGGGSFGIVVG
Sbjct: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVAN+MEE LLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKL+ IMNTT PNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSK 420
           +KPTLSNNISYKIKSDYVKQPIS+HAFKGIWDRL+S EVETSQLFLFPYGGKM NISSSK
Sbjct: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLD 480
           TPFSHRAEFLYKISYTVGWAEQG+GANERHLNWIRE YSFMTPFVSNSPRAAY+NYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 536
           IGTNNK GKTSYEEASIWG+KYFGNNFKRLVQVKT VDPSNFFRH+QSIPPLTLS
Sbjct: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535

BLAST of CSPI01G25200 vs. ExPASy TrEMBL
Match: A0A1S3BDG4 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488681 PE=3 SV=1)

HSP 1 Score: 1021.5 bits (2640), Expect = 1.2e-294
Identity = 497/535 (92.90%), Postives = 513/535 (95.89%), Query Frame = 0

Query: 1   MKFFRLNPLFLISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHIPIYTPINSSYSSIL 60
           MK F LNPL   SFT+VSL STQWSTASSYK+KDFL+CLSIHST IPIYTPINSSY+S L
Sbjct: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60

Query: 61  RSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120
           RSYAQNPRFSTQY TLKPL IIKPSHVSH+QSTVICSKSHGLQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 180
           DLPF+VVDLINLKSIEVDTTNNT WVQSGATIGELYYRIAEKSRTLAFPAGVCPTVG+GG
Sbjct: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180

Query: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVG 240
            FSGGGYGWLMRKYGLAADNVIDAYLVDANG VFD EAMGEGLFWAIRGGGGGSFGIVVG
Sbjct: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVAN+MEE LLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKL+ IMNTT PNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSK 420
           +KPTLSNNISYKIKSDYVKQPIS+HAFKGIWDRL+S EVETSQLFLFPYGGKM NISSSK
Sbjct: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLD 480
           TPFSHRAEFLYKISYTVGWAEQG+GANERHLNWIRE YSFMTPFVSNSPRAAY+NYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 536
           IGTNNK GKTSYEEASIWG+KYFGNNFKRLVQVKT VDPSNFFRH+QSIPPLTLS
Sbjct: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535

BLAST of CSPI01G25200 vs. ExPASy TrEMBL
Match: A0A6J1EGP1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111434010 PE=3 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 5.3e-221
Identity = 380/533 (71.29%), Postives = 440/533 (82.55%), Query Frame = 0

Query: 6   LNPLFLISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHIP--IYTPINSSYSSILRSY 65
           +N LF     I+S F+  W+ A ++K + FLQCLSIHS  I   IYTPINSSYSS+L SY
Sbjct: 6   MNALF-----ILSSFTVLWAAALAHKHEHFLQCLSIHSRDISKLIYTPINSSYSSLLTSY 65

Query: 66  AQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS-DL 125
           A NPR S    T  P VIIKPSH+SH+Q+ V+CSKSHG QIR RSGGHD EGLSYIS  L
Sbjct: 66  ALNPRVSIP-ATPNPHVIIKPSHLSHIQAAVVCSKSHGFQIRTRSGGHDAEGLSYISHHL 125

Query: 126 PFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHF 185
           PF++VDL NL SI V+  NNTAWVQSGATIGE+YY IA+KSRTLAFPAGVCPTVG GGH 
Sbjct: 126 PFIIVDLKNLNSITVNIENNTAWVQSGATIGEVYYEIAKKSRTLAFPAGVCPTVGFGGHL 185

Query: 186 SGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWK 245
           SGGGYGWLMRKYGLAADNV+DAYLVDA G V DREAMGE LFWAIRGGGGGSFGIVVGWK
Sbjct: 186 SGGGYGWLMRKYGLAADNVVDAYLVDAYGKVLDREAMGEDLFWAIRGGGGGSFGIVVGWK 245

Query: 246 VKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNP 305
           VKLV VP IVTTC+L+K+LD   KK+VYQWQYVAN+M+E LL+G+NLTGGNPT+G K N 
Sbjct: 246 VKLVAVPAIVTTCQLEKNLDGYAKKLVYQWQYVANKMDENLLLGVNLTGGNPTQGAKPNH 305

Query: 306 TASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLSK 365
            A+FFSL+LG +  L+  MNTT P LGLT  +C++TSWIQSTL+AAGFT+  PLE LL+K
Sbjct: 306 KATFFSLFLGNSHDLIATMNTTFPQLGLTNNDCQQTSWIQSTLLAAGFTSRDPLEFLLTK 365

Query: 366 PTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTP 425
            T  N ++YKIKSDYV +PIS+HAFKGIWDRLKS ++E++QL LFPYGG+M  I + +TP
Sbjct: 366 -TSFNTVAYKIKSDYVNKPISKHAFKGIWDRLKSPKMESAQLLLFPYGGRMSKIPTWETP 425

Query: 426 FSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIG 485
           FSHRAEF+YKISY VGWAEQ   A+ER+LNW+REFY +M+PFVSNSPRAAY+NYRDLDIG
Sbjct: 426 FSHRAEFMYKISYVVGWAEQSREASERNLNWVREFYEYMSPFVSNSPRAAYVNYRDLDIG 485

Query: 486 TNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 536
            NN+YG TSYEEA +WG  YFG+NFKRLV+VK  VDP NFFRHEQS+PPL LS
Sbjct: 486 MNNEYGSTSYEEARVWGEMYFGDNFKRLVRVKNKVDPLNFFRHEQSVPPLALS 531

BLAST of CSPI01G25200 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 2.7e-196
Identity = 343/538 (63.75%), Postives = 423/538 (78.62%), Query Frame = 0

Query: 5   RLNPLFLISFTIVSL-FSTQWSTASSYKSKDFLQCLSIHSTHIP-----IYTPINSSYSS 64
           + +PL ++S   + L  S+ W+ AS+ K + FLQCLS HS         IYTPIN SYSS
Sbjct: 2   KYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYSS 61

Query: 65  ILRSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSY 124
           +L    +N RFS+   T KPL+II PS+VSH+Q+ VICSK+HGLQIRIRSGGHD EGLSY
Sbjct: 62  VLNFSVRNLRFSSA-ETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY 121

Query: 125 ISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGI 184
           ++ LPF+++DLINLKSI V+  N+TAW+QSGAT+GELYY IA+KSRTLAFPAGVCPTVG+
Sbjct: 122 VAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGV 181

Query: 185 GGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIV 244
           GGHFSGGGYG ++RKYGLAADNVIDAYL+DANG  +DRE+MGE LFWAIRGGGGGSFGIV
Sbjct: 182 GGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 241

Query: 245 VGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTK-- 304
           V WKVKLV VP IVTTC  D++LD++T  ++++WQ VA +++E L  GI L GGN +   
Sbjct: 242 VAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEG 301

Query: 305 GVKR-NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNG-Q 364
           GVK+ NPTA+FFSL+LGK+D+L+ I+NT  P L L K +C ETSWI+STL+AA      +
Sbjct: 302 GVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIE 361

Query: 365 PLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMR 424
            LE LL++  L+   + K+KSDYVK+PI +   +GIW+RLK+Q++E  QL   PYGG+M 
Sbjct: 362 SLESLLNRTPLTIE-NTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMS 421

Query: 425 NISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYI 484
            IS S+TPFSHRA  LYKI Y VGW EQ   A ERHL+WIRE Y +MTPFVS SPRA Y+
Sbjct: 422 RISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYV 481

Query: 485 NYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
           NYRDLDIGTNNKYGKTSY++AS+WG KYFGNNF RLV +K+ VDPSNFF++EQSIPPL
Sbjct: 482 NYRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537

BLAST of CSPI01G25200 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 2.5e-194
Identity = 340/538 (63.20%), Postives = 413/538 (76.77%), Query Frame = 0

Query: 7   NPLFLISFTIVSLF---STQWSTASSYKSKDFLQCLSIHSTHI------PIYTPINSSYS 66
           +PL  +SF+++ +F   S+ W+ AS  K + FL CL  HS          +YTP+NSSYS
Sbjct: 5   SPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLNSSYS 64

Query: 67  SILRSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLS 126
           S+L    +N RFST  +T KPL II P HVSH+Q+ V+CS++HGLQIR RSGGHD EGLS
Sbjct: 65  SVLDFSIRNLRFSTA-DTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFEGLS 124

Query: 127 YISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVG 186
            +S LPF+++DLINL+SI +D  NNTAWVQSGAT+GELYY IAEKSRTLAFPAG CPTVG
Sbjct: 125 CVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACPTVG 184

Query: 187 IGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGI 246
           IGGHFSGGGYG ++RK+GLAADNVIDAYLVDANG   DRE MGE LFWAIRGGGGGSFGI
Sbjct: 185 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGI 244

Query: 247 VVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTK- 306
           VV WKVKLV VP  VT+C  +++L++   K++++WQYVAN+++E L +GI L GGN T  
Sbjct: 245 VVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQ 304

Query: 307 -GVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQP 366
            G K NPTASFFSL+LGK D+   I+NTT P LGL K NC E+SWI+S L++AGF  G  
Sbjct: 305 GGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSAGFKIGDS 364

Query: 367 LEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRN 426
           LE LL++  L+N  S KIKSDYVK+PIS+   +GIW+RLKS++V   QL   PYGG+M  
Sbjct: 365 LEALLNRTPLTNG-SIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGGRMSQ 424

Query: 427 ISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYIN 486
           IS S+ PFSHRA  LYKI Y V W +Q     +RHL WIRE YS+MTPFVS SPR+AY+N
Sbjct: 425 ISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRSAYVN 484

Query: 487 YRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLT 534
           YRDLDIG+NNKYGKTS++ AS+WG KYFGNNF RLV VKT VDP +FFRHEQSIP ++
Sbjct: 485 YRDLDIGSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTIS 540

BLAST of CSPI01G25200 vs. NCBI nr
Match: XP_004135741.1 (tetrahydrocannabinolic acid synthase [Cucumis sativus] >KAE8653321.1 hypothetical protein Csa_023149 [Cucumis sativus])

HSP 1 Score: 1069.7 bits (2765), Expect = 8.2e-309
Identity = 526/535 (98.32%), Postives = 529/535 (98.88%), Query Frame = 0

Query: 1   MKFFRLNPLFLISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHIPIYTPINSSYSSIL 60
           MKFF LNPLFLISFTIVSLFSTQWSTASSYK+KDFLQCLSIHSTHIPIYTPINSSYSSIL
Sbjct: 1   MKFFPLNPLFLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIPIYTPINSSYSSIL 60

Query: 61  RSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120
           RSYAQN RFSTQY TLKPLVIIKPSHVSHLQSTVICSKSH LQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 180
           DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG
Sbjct: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 180

Query: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVG 240
           HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGE LFWAIRGGGGGSFGIVVG
Sbjct: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSK 420
           SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKM NISSSK
Sbjct: 361 SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLD 480
           TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAY+NYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 536
           IGTNNKYGKTSYEEASIWGLKYFGNNFK+LVQVKTTVDPSNFFRHEQSIPPLTLS
Sbjct: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLTLS 535

BLAST of CSPI01G25200 vs. NCBI nr
Match: XP_008445749.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >KAA0047086.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa] >TYK05069.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1021.5 bits (2640), Expect = 2.6e-294
Identity = 497/535 (92.90%), Postives = 513/535 (95.89%), Query Frame = 0

Query: 1   MKFFRLNPLFLISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHIPIYTPINSSYSSIL 60
           MK F LNPL   SFT+VSL STQWSTASSYK+KDFL+CLSIHST IPIYTPINSSY+S L
Sbjct: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60

Query: 61  RSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120
           RSYAQNPRFSTQY TLKPL IIKPSHVSH+QSTVICSKSHGLQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 180
           DLPF+VVDLINLKSIEVDTTNNT WVQSGATIGELYYRIAEKSRTLAFPAGVCPTVG+GG
Sbjct: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180

Query: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVG 240
            FSGGGYGWLMRKYGLAADNVIDAYLVDANG VFD EAMGEGLFWAIRGGGGGSFGIVVG
Sbjct: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVAN+MEE LLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKL+ IMNTT PNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSK 420
           +KPTLSNNISYKIKSDYVKQPIS+HAFKGIWDRL+S EVETSQLFLFPYGGKM NISSSK
Sbjct: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLD 480
           TPFSHRAEFLYKISYTVGWAEQG+GANERHLNWIRE YSFMTPFVSNSPRAAY+NYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 536
           IGTNNK GKTSYEEASIWG+KYFGNNFKRLVQVKT VDPSNFFRH+QSIPPLTLS
Sbjct: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535

BLAST of CSPI01G25200 vs. NCBI nr
Match: XP_038895165.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 934.5 bits (2414), Expect = 4.1e-268
Identity = 456/536 (85.07%), Postives = 490/536 (91.42%), Query Frame = 0

Query: 1   MKFFRLNPLF-LISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHIPIYTPINSSYSSI 60
           MK F LNPL    +F IVS F  QW+ ASSYK +DFLQCLSI+S  IPIYTPINSSYSS+
Sbjct: 1   MKVFPLNPLLTFTNFIIVSSFLVQWTAASSYKHEDFLQCLSIYSRDIPIYTPINSSYSSL 60

Query: 61  LRSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYI 120
           L SYAQN RFSTQ+ TLKPLVIIKPSH+SH+Q+ VICSKSHGLQIRIRSGGHDTEGLSYI
Sbjct: 61  LTSYAQNTRFSTQHKTLKPLVIIKPSHISHIQAIVICSKSHGLQIRIRSGGHDTEGLSYI 120

Query: 121 SDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIG 180
           SD+PF+V+DLINLKSIEVDT NNTAWVQSGATIGELYY+IAEKSRTLAFPAGVCPTVGIG
Sbjct: 121 SDVPFIVIDLINLKSIEVDTKNNTAWVQSGATIGELYYKIAEKSRTLAFPAGVCPTVGIG 180

Query: 181 GHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVV 240
           GHFSGGGYGWLMRKYGLAADNV+DAYLVD +G VFDRE MGE LFWAIRGGGGGSFGIVV
Sbjct: 181 GHFSGGGYGWLMRKYGLAADNVMDAYLVDDDGRVFDRETMGEDLFWAIRGGGGGSFGIVV 240

Query: 241 GWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVK 300
           GWKVKLVRVPPIVTTCRL+KSLDKNTKKIVYQWQYVAN+ME+ LLIGINLTGGNPT+GVK
Sbjct: 241 GWKVKLVRVPPIVTTCRLEKSLDKNTKKIVYQWQYVANKMEDTLLIGINLTGGNPTQGVK 300

Query: 301 RNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEIL 360
           RNPTASFFSL+LGKT+KL+TIMNTT P LGLTK+NC+ETSWIQSTLI+AGFTNG+ LE+L
Sbjct: 301 RNPTASFFSLFLGKTNKLITIMNTTFPELGLTKSNCEETSWIQSTLISAGFTNGEALEVL 360

Query: 361 LSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSS 420
           L+KP +   ISYKIKSDYVKQPISQ A KGIW+RLKSQ  ETSQLFLFPYGGKM  ISSS
Sbjct: 361 LAKPPI-KTISYKIKSDYVKQPISQQALKGIWERLKSQVTETSQLFLFPYGGKMSKISSS 420

Query: 421 KTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDL 480
           K PFSHRAEFLYKISYTVGW EQ   ANERHLNWIRE Y +MTPFVSNSPRAAYINYRDL
Sbjct: 421 KIPFSHRAEFLYKISYTVGWTEQSIVANERHLNWIRELYDYMTPFVSNSPRAAYINYRDL 480

Query: 481 DIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 536
           DIGTNNKYG TSY++ASIWGLKYFGNNFKRLVQVKT VDPSNFFRHEQSIPPL+LS
Sbjct: 481 DIGTNNKYGNTSYKQASIWGLKYFGNNFKRLVQVKTKVDPSNFFRHEQSIPPLSLS 535

BLAST of CSPI01G25200 vs. NCBI nr
Match: XP_023520034.1 (tetrahydrocannabinolic acid synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 786.9 bits (2031), Expect = 1.1e-223
Identity = 386/533 (72.42%), Postives = 442/533 (82.93%), Query Frame = 0

Query: 6   LNPLFLISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHIP--IYTPINSSYSSILRSY 65
           +N LF     I+S FS  W  A ++K + FLQCLSIHS  I   IYTPINSSYSS+L SY
Sbjct: 6   MNALF-----ILSSFSVLWVVALAHKHEHFLQCLSIHSRDISKVIYTPINSSYSSLLSSY 65

Query: 66  AQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS-DL 125
           A NPR ST   T  P VIIKPSH+SH+Q+ V+CSKSHG +IR RSGGHD EGLSYIS  L
Sbjct: 66  ALNPRVSTP-ATPNPRVIIKPSHLSHIQAAVVCSKSHGFRIRTRSGGHDAEGLSYISHHL 125

Query: 126 PFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHF 185
           PF+VVDL NL SI VD  NNTAWVQSGATIGE+YY IA+KSRTLAFPAGVCPTVG GGH 
Sbjct: 126 PFIVVDLKNLNSIAVDIENNTAWVQSGATIGEVYYEIAKKSRTLAFPAGVCPTVGFGGHL 185

Query: 186 SGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWK 245
           SGGGYGWLMRKYGLAADNV+DAYLVDA G V DREAMGE LFWAIRGGGGGSFGIVVGWK
Sbjct: 186 SGGGYGWLMRKYGLAADNVVDAYLVDAYGKVLDREAMGEDLFWAIRGGGGGSFGIVVGWK 245

Query: 246 VKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNP 305
           VKLV VP IVTTC+L+K+LD   KK+VYQWQYVAN+M+E LL+G+NLTGGNPT+G K N 
Sbjct: 246 VKLVAVPAIVTTCQLEKNLDGYAKKLVYQWQYVANKMDENLLLGVNLTGGNPTEGAKPNH 305

Query: 306 TASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLSK 365
            A+FFSL+LG + +L+  MNTT P LGLT  +C++TSWIQSTL+AAGFTN  PLE LL+K
Sbjct: 306 KATFFSLFLGNSHQLIATMNTTFPQLGLTNNDCQQTSWIQSTLLAAGFTNRDPLEFLLTK 365

Query: 366 PTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTP 425
            T  N ++YKIKSDYV +PIS+HAFKGIWDRLKS ++E++QL LFPYGG+M  I + +TP
Sbjct: 366 -TSFNTVAYKIKSDYVNKPISKHAFKGIWDRLKSPKMESAQLLLFPYGGRMSQIPTWETP 425

Query: 426 FSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIG 485
           FSHRAEF+YKISY VGWAEQ   ANER+LNW+REFY +M+PFVSNSPRAAY+NYRDLDIG
Sbjct: 426 FSHRAEFMYKISYVVGWAEQSMEANERNLNWVREFYEYMSPFVSNSPRAAYVNYRDLDIG 485

Query: 486 TNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 536
            NN+YG TSYEEA +WG  YFG+NFKRLV+VK  VDP NFFRHEQS+PPLTLS
Sbjct: 486 MNNEYGSTSYEEARVWGEMYFGDNFKRLVRVKNKVDPLNFFRHEQSVPPLTLS 531

BLAST of CSPI01G25200 vs. NCBI nr
Match: XP_022927076.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 776.9 bits (2005), Expect = 1.1e-220
Identity = 380/533 (71.29%), Postives = 440/533 (82.55%), Query Frame = 0

Query: 6   LNPLFLISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHIP--IYTPINSSYSSILRSY 65
           +N LF     I+S F+  W+ A ++K + FLQCLSIHS  I   IYTPINSSYSS+L SY
Sbjct: 6   MNALF-----ILSSFTVLWAAALAHKHEHFLQCLSIHSRDISKLIYTPINSSYSSLLTSY 65

Query: 66  AQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS-DL 125
           A NPR S    T  P VIIKPSH+SH+Q+ V+CSKSHG QIR RSGGHD EGLSYIS  L
Sbjct: 66  ALNPRVSIP-ATPNPHVIIKPSHLSHIQAAVVCSKSHGFQIRTRSGGHDAEGLSYISHHL 125

Query: 126 PFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHF 185
           PF++VDL NL SI V+  NNTAWVQSGATIGE+YY IA+KSRTLAFPAGVCPTVG GGH 
Sbjct: 126 PFIIVDLKNLNSITVNIENNTAWVQSGATIGEVYYEIAKKSRTLAFPAGVCPTVGFGGHL 185

Query: 186 SGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWK 245
           SGGGYGWLMRKYGLAADNV+DAYLVDA G V DREAMGE LFWAIRGGGGGSFGIVVGWK
Sbjct: 186 SGGGYGWLMRKYGLAADNVVDAYLVDAYGKVLDREAMGEDLFWAIRGGGGGSFGIVVGWK 245

Query: 246 VKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNP 305
           VKLV VP IVTTC+L+K+LD   KK+VYQWQYVAN+M+E LL+G+NLTGGNPT+G K N 
Sbjct: 246 VKLVAVPAIVTTCQLEKNLDGYAKKLVYQWQYVANKMDENLLLGVNLTGGNPTQGAKPNH 305

Query: 306 TASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLSK 365
            A+FFSL+LG +  L+  MNTT P LGLT  +C++TSWIQSTL+AAGFT+  PLE LL+K
Sbjct: 306 KATFFSLFLGNSHDLIATMNTTFPQLGLTNNDCQQTSWIQSTLLAAGFTSRDPLEFLLTK 365

Query: 366 PTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTP 425
            T  N ++YKIKSDYV +PIS+HAFKGIWDRLKS ++E++QL LFPYGG+M  I + +TP
Sbjct: 366 -TSFNTVAYKIKSDYVNKPISKHAFKGIWDRLKSPKMESAQLLLFPYGGRMSKIPTWETP 425

Query: 426 FSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIG 485
           FSHRAEF+YKISY VGWAEQ   A+ER+LNW+REFY +M+PFVSNSPRAAY+NYRDLDIG
Sbjct: 426 FSHRAEFMYKISYVVGWAEQSREASERNLNWVREFYEYMSPFVSNSPRAAYVNYRDLDIG 485

Query: 486 TNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 536
            NN+YG TSYEEA +WG  YFG+NFKRLV+VK  VDP NFFRHEQS+PPL LS
Sbjct: 486 MNNEYGSTSYEEARVWGEMYFGDNFKRLVRVKNKVDPLNFFRHEQSVPPLALS 531

BLAST of CSPI01G25200 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 484.2 bits (1245), Expect = 1.4e-136
Identity = 248/542 (45.76%), Postives = 347/542 (64.02%), Query Frame = 0

Query: 8   PLFLISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHIPIYTPIN----------SSYS 67
           PLF I     SL++   +++ +     F+ C+   +TH  +Y P+           S +S
Sbjct: 8   PLFSILVLYFSLYTITPTSSLASLQDQFINCVQ-RNTH--VYFPLEKTFFAPTKNVSMFS 67

Query: 68  SILRSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLS 127
            +L S AQN RF  + +  KP  I  P H SH+Q+++ICSK   + +R+RSGGHD EGLS
Sbjct: 68  QVLESTAQNLRF-LKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLS 127

Query: 128 YIS--DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPT 187
           Y+S  D PF+++DL  ++ + ++  +N+AWVQSGAT+GELYYRIAEKS+   FPAG+C +
Sbjct: 128 YVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSS 187

Query: 188 VGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSF 247
           +GIGGH +GG YG +MRKYGL ADNV+DA +VDANG + DR AMGE  FWAIRGG GGSF
Sbjct: 188 LGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSF 247

Query: 248 GIVVGWKVKLVRVPPIVTTCRLDKSLDKNT-KKIVYQWQYVANRMEEKLLIGINL----T 307
           GI++ WK+KLV VP  VT   + K+L ++   KI+ +WQ VA+++ E+L I +      T
Sbjct: 248 GIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGT 307

Query: 308 GGNPTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGF 367
           GGN T       T S+ +L+LG    L+ +M  + P LGLT  +C E SW++S    +GF
Sbjct: 308 GGNKT------VTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGF 367

Query: 368 TNGQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYG 427
               P  +LL   +    +S+K KSD+VK PI +   +GI+ +L  +++    +   PYG
Sbjct: 368 PTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYG 427

Query: 428 GKMRNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPR 487
           G M  I  S+ PF HR   L+K+ Y   W +     + RH+NWIR+ YS+MTP+VS++PR
Sbjct: 428 GMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRPS-RHINWIRDLYSYMTPYVSSNPR 487

Query: 488 AAYINYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIP 533
            AY+NYRDLD+G N K  KT  ++A +WG  YF NNF RL+ +K  VDP NFFRHEQSIP
Sbjct: 488 EAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 536

BLAST of CSPI01G25200 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 483.4 bits (1243), Expect = 2.3e-136
Identity = 250/530 (47.17%), Postives = 342/530 (64.53%), Query Frame = 0

Query: 10  FLISFTIVSLFSTQWSTASSYKSKDFLQCLSIH---STHIP--IYTPINSSYSSILRSYA 69
           FL +  + SL  +   +A     +DFL+CLS     +T  P  I+T  +SS+ SIL S  
Sbjct: 6   FLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSI 65

Query: 70  QNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS-DLP 129
           QNPRFS    T KP+ II P   S +Q+ + C++ HG+ +R RS GH  EGLSYI+ + P
Sbjct: 66  QNPRFSVS-ETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 125

Query: 130 FVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFS 189
           F V+DL NL+SI +D  N T WVQ+GAT GELYY I + +++LAFPAG+ PTVG+GG FS
Sbjct: 126 FAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFS 185

Query: 190 GGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKV 249
           GGGYG L+RKYGLAADN+IDA +VDA+G + DR+AMGE  FWAIRGGGG SFG+++ WKV
Sbjct: 186 GGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKV 245

Query: 250 KLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPT 309
           KLV VP  +T  ++ K+  K   +I+ +WQY A+++ + L I   L   N     K    
Sbjct: 246 KLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN-----KNAVH 305

Query: 310 ASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLSKP 369
           A F  LY+G  + L+ +M    P LGL K  C+E SWI+S L  A F  G+ L +L ++ 
Sbjct: 306 ALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLTNRE 365

Query: 370 TLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTPF 429
             S  +S+K K D+V++PI + A + IW RL++ E    ++ L P+GGKM  ++  +TPF
Sbjct: 366 RTS--LSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPF 425

Query: 430 SHRAEFLYKISYTVGWAEQ---GSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLD 489
            HR   LY+I Y   W E+        +++L W+   Y FMTP+VS SPR AY+N++D+D
Sbjct: 426 PHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMD 485

Query: 490 IGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIP 531
           +G      KT YEE   WG+KYF NNF+RLV+VKT VDP++FF  EQSIP
Sbjct: 486 LGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of CSPI01G25200 vs. TAIR 10
Match: AT5G44360.1 (FAD-binding Berberine family protein )

HSP 1 Score: 481.1 bits (1237), Expect = 1.2e-135
Identity = 253/536 (47.20%), Postives = 353/536 (65.86%), Query Frame = 0

Query: 10  FLISFTIVSLFSTQWSTASSYKSKD-FLQCLSIHS------THIPIYTPINSSYSSILRS 69
           F +S  +V L     S +SS  SKD FL C+S HS          I+ P +  Y+   +S
Sbjct: 7   FALSLFLVFLVKWVNSDSSSSPSKDQFLSCMSTHSDSSFINPKSFIHKPDSRVYTDFSQS 66

Query: 70  -YAQNPRFST-QYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 129
             +QN RF T  + + KP++I+ P   + +Q +++CS+  G+++R +SGGHD EGLSY+S
Sbjct: 67  LISQNYRFLTLNFTSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRTKSGGHDYEGLSYLS 126

Query: 130 -DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIG 189
              PF+++DL+N++SIE++  + TAWV +GATIGELYY+IA+ S+   FPAG CP+VG+G
Sbjct: 127 LHSPFIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTCPSVGVG 186

Query: 190 GHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFD-REAMGEGLFWAIRGGGGGSFGIV 249
           GHFSGGG+G +MRK+GLAADNV+DA  VDANG +++ R  MGE LFWAIRGGG  SFG+V
Sbjct: 187 GHFSGGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGAASFGVV 246

Query: 250 VGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIG--INLTGGNPTK 309
           + WKVKLVRVP  VT  R +  L +N  KIV++WQ +A  +++ L I   ++++GG    
Sbjct: 247 LSWKVKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVSISGG---- 306

Query: 310 GVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPL 369
               +   +F + YLG  DKL+ +MN   P LGLT  +C E +WI S ++   +  GQPL
Sbjct: 307 ----SVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDS-IMYFNWKKGQPL 366

Query: 370 EILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNI 429
           E LL +    N++ +K KSD+VK PI +   +GIW R    EVE+  + + P GGKM  I
Sbjct: 367 ETLLDRGQRYNDLYFKAKSDFVKNPIPEIGLEGIWTRF--HEVESPIMIMEPLGGKMYEI 426

Query: 430 SSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINY 489
             ++TPF HR   LY I Y V W  +  G  E+H+ W+R  Y +M  +VS SPR AY+NY
Sbjct: 427 GETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRGAYLNY 486

Query: 490 RDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
           RDLD+G N     TS+E+A +WG +YFG+NFKRL  VK  +DP+NFFR+EQS+PPL
Sbjct: 487 RDLDLGMNRGV-NTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPPL 530

BLAST of CSPI01G25200 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 480.7 bits (1236), Expect = 1.5e-135
Identity = 247/532 (46.43%), Postives = 352/532 (66.17%), Query Frame = 0

Query: 9   LFLISFTIVSLFSTQWSTASSYKSKDFLQCLSI-----HSTHIPIYTPINSSYSSILRSY 68
           L L+ F +V ++    S++SS  S+ F QCL+      H     I+   N SYSS+L++ 
Sbjct: 5   LILVLFFVVFIWQ---SSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQAN 64

Query: 69  AQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYI--SD 128
            +N RF+T  +T KP +II  +H SH+Q+ + C K H LQ++IRSGGHD +GLSY+  S 
Sbjct: 65  IRNLRFNTT-STPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSG 124

Query: 129 LPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGH 188
            PF V+D+ NL+S++VD  + TAWVQ+GA +GE+YY I EKS+TLA+PAG+CPTVG+GGH
Sbjct: 125 KPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGH 184

Query: 189 FSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGW 248
            SGGGYG +MRKYGL  DN IDA +VD NG + DR+ MGE L+WAI GGGGGS+G+V+ +
Sbjct: 185 ISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAY 244

Query: 249 KVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRN 308
           K+ LV VP  VT  R+ ++L++N   I+++WQ VA ++ ++L I   +   N T   ++ 
Sbjct: 245 KINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKT 304

Query: 309 PTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLS 368
              +F +++LG T  L++I+N   P LGL +++C ETSWIQS L       G    +LL 
Sbjct: 305 VRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQ 364

Query: 369 KPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKT 428
           +    N +  K KSDYV++PIS+   + IW ++   E+ T  +   PYGG+M  ISS+ T
Sbjct: 365 RNQPVNYL--KRKSDYVREPISRTGLESIWKKMIELEIPT--MAFNPYGGEMGRISSTVT 424

Query: 429 PFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDI 488
           PF +RA  L+KI Y   W ++     +R++   R+ Y FMTPFVS +PR ++ NYRD+D+
Sbjct: 425 PFPYRAGNLWKIQYGANWRDE--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDL 484

Query: 489 GTNNKYGK-TSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
           G N+  GK +SY E   +G KYF  NF+RLV++KT VD  NFFR+EQSIP L
Sbjct: 485 GINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of CSPI01G25200 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 477.6 bits (1228), Expect = 1.3e-134
Identity = 253/540 (46.85%), Postives = 349/540 (64.63%), Query Frame = 0

Query: 1   MKFFRLNPLFLISFTIVSLFSTQWSTASSYKS--KDFLQCLS--IHSTHI---PIYTPIN 60
           MKF       LI FT  +L  +     S+ +S    FLQCLS  ++ ++I    I+TP +
Sbjct: 1   MKFQSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPND 60

Query: 61  SSYSSILRSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDT 120
           +S+SS+L S  QN RFS   +  KP++I+ P   S +QS V C++  G+ IR RSGGHD 
Sbjct: 61  TSFSSVLASSIQNQRFSAP-DVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDY 120

Query: 121 EGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVC 180
           EGLSY++  PFV++DL NL+SI VD  N + WVQ+GATIGELYY I +K+RTLAFPAGVC
Sbjct: 121 EGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVC 180

Query: 181 PTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGG 240
           PTVG+GGHFSGGGYG L+RK+GLAAD+VIDA +VDA G + +R  MGE  FWAIRGGGG 
Sbjct: 181 PTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGS 240

Query: 241 SFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGN 300
           SF +V+ WK+ L+ VP  VT   + K  +++  KI+++WQ+VA+++ + L I + L    
Sbjct: 241 SFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML---- 300

Query: 301 PTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNG 360
             +  K    ASF  LYLG    L+ ++N   P LGL + +C E SWI+S +  A     
Sbjct: 301 --QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAEL-GE 360

Query: 361 QPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKM 420
           +P+ +L  +   S  +++K KSD+V++P+ + A   +W RL+  E E +QL   P+GGKM
Sbjct: 361 EPINVLTKRTRAS--LAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKM 420

Query: 421 RNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAY 480
             I+  +TPF HR   +Y+I Y   W        E+++ W+   Y  M+ FV+ SPR AY
Sbjct: 421 SEIADYETPFPHRKGNIYEIQYLNYWR---GDVKEKYMRWVERVYDDMSEFVAKSPRGAY 480

Query: 481 INYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLT 534
           IN RDLD+G      ++ YEE   WG+KYF NNF+RLV+VKT+VDPS+FF  EQSIPP T
Sbjct: 481 INLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPPFT 527