Homology
BLAST of CSPI01G23580 vs. ExPASy Swiss-Prot
Match:
F4HQM3 (Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1)
HSP 1 Score: 1163.3 bits (3008), Expect = 0.0e+00
Identity = 563/748 (75.27%), Postives = 636/748 (85.03%), Query Frame = 0
Query: 34 VLSESKYLIGLGSHDITGPAADVNMMGYANADQIASGIHFRLRARAFIVAEPQGKRVVFV 93
V S S+YLIGLGS+DITGPAADVNMMGYAN +Q+ASGIHFRLRAR FIV+EPQGKRVVFV
Sbjct: 31 VYSHSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSEPQGKRVVFV 90
Query: 94 NLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFV 153
NLDACMASQIV +KV+ERLKARYGDLYTE+NV ISGIH+HAGPGGYLQYVVYIVTSLGFV
Sbjct: 91 NLDACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFV 150
Query: 154 RQSFNVLVDGIEKSIIQAHENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYD 213
RQSF+ LVDGIE SIIQAHENL PGSI +N GEL+DAGVNRSPSAYLNNP+ ERSK+KY+
Sbjct: 151 RQSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYN 210
Query: 214 VDKEMTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTG 273
VDKEMTLLKF+DD+WGPVG+FNWFATHGTSMSRTN+LISGDNKGAA+RFMEDW++Q
Sbjct: 211 VDKEMTLLKFVDDQWGPVGSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAE 270
Query: 274 TLHPGESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLR 333
+ E +D IPRRVS+++ ELLELA+ F+SQPG+P TR+ S + RVR+ LR
Sbjct: 271 RSYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRISSSARRVRSALR 330
Query: 334 QADRPQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEF 393
+AD+P FVSAFCQ+NCGDVSPN LGAFCLDTGLPCDFNHSTCGGKNE+CYGRGPGYPDEF
Sbjct: 331 KADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEF 390
Query: 394 ESTRIIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPA 453
ESTRIIGE+QF+ A++LF+KASEQL GKVD+RH YVDFS L V+L K+ G +EVVKTCPA
Sbjct: 391 ESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPA 450
Query: 454 AMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEM 513
AMGFAFAAGTTDGPGAFDF QGDDKGN FW+LVRNVLK P +QI C PKPILLDTGEM
Sbjct: 451 AMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEM 510
Query: 514 KTPYDWAPSILPIQILRIGQLVILNVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHV 573
PYDWAPSIL +Q+LRIGQL IL+VPGEFTTMAGRRLR AVKT L K+ + +HV
Sbjct: 511 TKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHV 570
Query: 574 VIAGLTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGP 633
VIAGL N YSQYVTTFEEY++QRYEGASTLYGPHTL YIQEFKKL++SL+ PV PGP
Sbjct: 571 VIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLVLDMPVQPGP 630
Query: 634 QPPDLLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGN-LVKVTFWTGCPRNDL 693
QPPDLL +Q+S L PV++D TP G SFGDV DVP N S KRGN V V F + CPRNDL
Sbjct: 631 QPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDVPKNLSLKRGNGQVTVVFRSACPRNDL 690
Query: 694 MTEGTFALVEILQK--NTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGV 753
+TEGTF LVE L++ TW P YDDDD CLRFKWSR LS++S AT+EWRIP++A GV
Sbjct: 691 LTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESASPGV 750
Query: 754 YRIRHFGAAKSLLGSIRHFTGSSSAFVV 779
YRI HFGAAK L GS+ HFTGSSSAFVV
Sbjct: 751 YRITHFGAAKKLFGSVHHFTGSSSAFVV 778
BLAST of CSPI01G23580 vs. ExPASy Swiss-Prot
Match:
Q304B9 (Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1)
HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 556/758 (73.35%), Postives = 655/758 (86.41%), Query Frame = 0
Query: 22 IYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMGYANADQIASGIHFRLRARAFI 81
I L+L +R+V + YLIG+GS+DITGPAADVNMMGYAN+DQIASGIHFRLRARAFI
Sbjct: 11 ILFLLLLLLSRTVYA---YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFI 70
Query: 82 VAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHSHAGPGGYLQ 141
VAEPQG RVVFVNLDACMASQIVTIKVLERLKARYG+LYTEKNVAISGIH+HAGPGGYLQ
Sbjct: 71 VAEPQGNRVVFVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQ 130
Query: 142 YVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSILINKGELIDAGVNRSPSAYLN 201
YV YIVTSLGFVRQSF+V+V+GIE+SI+QAHE+L PGS +NKG+L+DAGVNRSPS+YLN
Sbjct: 131 YVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLN 190
Query: 202 NPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAAR 261
NPA+ERSKYKYDVDKEMTL+KF+D + GP G+FNWFATHGTSMSRTN+LISGDNKGAAAR
Sbjct: 191 NPAAERSKYKYDVDKEMTLVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGAAAR 250
Query: 262 FMEDWFKQKGTGTLHPGESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRV 321
FMEDWF+ ++ + +IPRRVS IV + +++ LL++AA+++S G +
Sbjct: 251 FMEDWFENGQKNSV-----SSRNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKS 310
Query: 322 LSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNEL 381
L + +RVRN + +FVSAFCQSNCGDVSPNTLG FC+DTGLPCDFNHSTC G+NEL
Sbjct: 311 LDVKTRVRN----GSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNEL 370
Query: 382 CYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQ 441
CYGRGPGYPDEFESTRIIGEKQF+ AV+LF+KA+E+L GK+ ++H+Y+DFS+L+V++ K
Sbjct: 371 CYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKA 430
Query: 442 GGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQ 501
GG +E VKTCPAAMGF FAAGTTDGPGAFDFKQGDD+GN FW+LVRNVL+ PG EQ+ CQ
Sbjct: 431 GGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQ 490
Query: 502 SPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILNVPGEFTTMAGRRLRDAVKTVLTT 561
PKPILLDTGEMK PYDWAPSILPIQILRIGQLVIL+VPGEFTTMAGRRLRDA+K+ L +
Sbjct: 491 KPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLIS 550
Query: 562 GAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQ 621
KEF++N+HVVIAGLTNTYSQY+ TFEEY++QRYEGASTLYG HTL AYIQEFKKLA
Sbjct: 551 SDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLAT 610
Query: 622 SLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKV 681
+L++G + GPQPPDLL +QISLL PV++D TPLGV FGDVK DVP S+F+RG V
Sbjct: 611 ALVNGLTLPRGPQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADVPPKSTFRRGQQVNA 670
Query: 682 TFWTGCPRNDLMTEGTFALVEILQK-NTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEW 741
TFW+GCPRNDLMTEG+FA+VE L++ W P YDDDDF L+FKWSRPA LS++S ATIEW
Sbjct: 671 TFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEW 730
Query: 742 RIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 779
R+P++AV+GVYRIRH+GA+KSL GSI F+GSSSAFVV
Sbjct: 731 RVPESAVAGVYRIRHYGASKSLFGSISSFSGSSSAFVV 756
BLAST of CSPI01G23580 vs. ExPASy Swiss-Prot
Match:
Q0JL46 (Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1 SV=1)
HSP 1 Score: 1127.5 bits (2915), Expect = 0.0e+00
Identity = 546/776 (70.36%), Postives = 646/776 (83.25%), Query Frame = 0
Query: 12 GRSLGTIWLCIYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMGYANADQIASGI 71
G S +WL + +++L+ S VLS S YL+G+GS DITGPAADVNMMGYAN +QIASGI
Sbjct: 15 GSSRVWLWLLLALVLLNCS--LVLSASPYLVGMGSFDITGPAADVNMMGYANTEQIASGI 74
Query: 72 HFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIH 131
HFRL++RAFIVAEP GKRVVFVN+DACMASQIVTIKVLERLKARYGDLY E NVAISGIH
Sbjct: 75 HFRLKSRAFIVAEPNGKRVVFVNIDACMASQIVTIKVLERLKARYGDLYNENNVAISGIH 134
Query: 132 SHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSILINKGELIDAG 191
+HAGPGGYLQYVVYIVTSLGFVRQSF+V+VDGIE+SI++AH NL PG I +NKG+L+DAG
Sbjct: 135 THAGPGGYLQYVVYIVTSLGFVRQSFDVIVDGIEQSIVEAHNNLRPGKIFVNKGDLLDAG 194
Query: 192 VNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALI 251
VNRSPSAYLNNPA ERSKY+Y+VDKEMTL+KF+DDE GPVG+FNWFATHGTSMSRTN+LI
Sbjct: 195 VNRSPSAYLNNPAEERSKYEYNVDKEMTLIKFVDDELGPVGSFNWFATHGTSMSRTNSLI 254
Query: 252 SGDNKGAAARFMEDWFKQKG--TGTLHPGESEADS------IPRRVSNIVPEVYKDKQEL 311
SGDNKGAAARFMEDW +Q G + H + S +PRRVS I+PE + +L
Sbjct: 255 SGDNKGAAARFMEDWAEQMGLPKQSAHANSDDLRSLHKTSVLPRRVSTIIPEPNEITDDL 314
Query: 312 LELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCLD 371
++LA+S+++ GR SI+ R+R+ Q ++P+FVSAFCQSNCGDVSPN LG FC+D
Sbjct: 315 IQLASSYEASGGRRLAG-SSITRRIRST--QQNKPKFVSAFCQSNCGDVSPNVLGTFCID 374
Query: 372 TGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTGKVD 431
T LPCDFNHSTC GKNELCYGRGPGYPDEFESTR+IG +QF KA DLF ASE++ GK+D
Sbjct: 375 TNLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRVIGNRQFLKARDLFDSASEEIQGKID 434
Query: 432 FRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFW 491
+RH+Y+DFS LEV ++ G + VKTCPAAMGFAFAAGTTDGPGAFDF+QGD KGN FW
Sbjct: 435 YRHTYLDFSKLEVKVSTSAGGQQTVKTCPAAMGFAFAAGTTDGPGAFDFRQGDVKGNPFW 494
Query: 492 KLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILNVPGEF 551
KLVRN+LK PG +Q+ C SPKPILLDTGEMK PYDWAP+ILP+Q++RIGQLVIL VPGEF
Sbjct: 495 KLVRNLLKTPGKDQVECHSPKPILLDTGEMKEPYDWAPAILPVQMIRIGQLVILCVPGEF 554
Query: 552 TTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGASTL 611
TTMAGRRLRDAVKTVLT+G EF+ N+HVV+AGLTN+YSQY+TTFEEY++QRYEGASTL
Sbjct: 555 TTMAGRRLRDAVKTVLTSG-NSEFDKNIHVVLAGLTNSYSQYITTFEEYQIQRYEGASTL 614
Query: 612 YGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSFGDV 671
YGPHTL AYIQEF+KLA ++I V QPPD+L +QI LLP V+ D TPLGV FGDV
Sbjct: 615 YGPHTLSAYIQEFQKLAMAMIANKEVPTNFQPPDMLDKQIGLLPGVVFDSTPLGVKFGDV 674
Query: 672 KFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQ-KNTWVPAYDDDDFCLRF 731
DVP NS+F +G+ V TF++ CPRNDL+T+GTFALVE L N WVP YDDDD+ LRF
Sbjct: 675 NSDVPGNSTFNKGSTVNATFYSACPRNDLLTDGTFALVEKLDGNNNWVPVYDDDDWSLRF 734
Query: 732 KWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 779
KWSRPA LS++S+AT+EW +P+ A +GVYR+RHFGA+K + GS+RHFTG+S AF V
Sbjct: 735 KWSRPARLSSRSFATLEWTVPEDAAAGVYRLRHFGASKPMFGSVRHFTGTSRAFAV 784
BLAST of CSPI01G23580 vs. ExPASy Swiss-Prot
Match:
F4KHQ8 (Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1)
HSP 1 Score: 1064.3 bits (2751), Expect = 6.6e-310
Identity = 531/762 (69.69%), Postives = 614/762 (80.58%), Query Frame = 0
Query: 21 CIYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMGYANADQIASGIHFRLRARAF 80
C L+ + S+S YL+GLGS+DITGPAADVNMMGYAN +Q+ASG+HFRLRARAF
Sbjct: 10 CTLFLLFLLRLTCIFSDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAF 69
Query: 81 IVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHSHAGPGGYL 140
IVAEP KR+ FVNLDA MASQ+VTIKV+ERLK RYG+LYTE+NVAISG H+HAGPGGYL
Sbjct: 70 IVAEPYKKRIAFVNLDAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYL 129
Query: 141 QYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSILINKGELIDAGVNRSPSAYL 200
QY++Y+VTSLGFV QSFN LVDGIE+SIIQAHENL PGSILINKGEL+DAGVNRSPSAYL
Sbjct: 130 QYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYL 189
Query: 201 NNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAA 260
NNPA ERSKY+YDVDKEMTL+KF+DD+WGPV A
Sbjct: 190 NNPAHERSKYEYDVDKEMTLVKFVDDQWGPV----------------------------A 249
Query: 261 RFMEDWFKQKGTGTLHPGESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATR 320
R MEDWF+++ ES PRRVS+I+ + Y Q+L+E+A+S S G+ TR
Sbjct: 250 RIMEDWFERENGCRSVDVES-----PRRVSSIISDPY--DQDLMEMASSLLSTGGKTVTR 309
Query: 321 VLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNE 380
+ S++ RVR+ R AD+P+FVSAFCQ+NCGDVSPN LGAFC+DTGLPC+FN STCGGKNE
Sbjct: 310 MSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNE 369
Query: 381 LCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNK 440
CYGRGPGYPDEFESTRIIGE+QF+KA DLF+KASE++ GKVD+RH+YVDFS LEV++N
Sbjct: 370 QCYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTING 429
Query: 441 QGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISC 500
Q G +EVVKTCPAAMGF FAAGTTDGPGAFDFKQGDD+GN FW+LVRN+LK P EQ+ C
Sbjct: 430 QNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRC 489
Query: 501 QSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILNVPGEFTTMAGRRLRDAVKTVLT 560
Q PKPILLDTGEMK PYDWAPSILP+QILRIGQLVIL VPGEFTTMAGRRLRDAVKTVL
Sbjct: 490 QRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLK 549
Query: 561 TGAK-KEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKL 620
G+ +EF+ VVIAGLTN+YSQY+ TFEEY++QRYEGASTLYGPHTL YIQEFKKL
Sbjct: 550 EGSNGREFS----VVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKL 609
Query: 621 AQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRG-NL 680
A L+ PGPQPPDLL +QISLL PV+ DMTP+G +FGDV DVP S F++G ++
Sbjct: 610 ANDLLSAQTTDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDVPRLSKFRKGADI 669
Query: 681 VKVTFWTGCPRNDLMTEGTFALVE--ILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYA 740
V+V F + PRNDLMTEGTFALVE + + TWVP YDDDDFCLRFKWSRP LS QS A
Sbjct: 670 VRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQSTA 729
Query: 741 TIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 779
TIEWRIP+TA GVYRI HFG+AK+ + SI HF+GSSSAFVV
Sbjct: 730 TIEWRIPETASPGVYRITHFGSAKTPISSIHHFSGSSSAFVV 732
BLAST of CSPI01G23580 vs. ExPASy Swiss-Prot
Match:
Q55G11 (Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1)
HSP 1 Score: 602.4 bits (1552), Expect = 7.1e-171
Identity = 339/773 (43.86%), Postives = 457/773 (59.12%), Query Frame = 0
Query: 14 SLGTIWLCIYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMGYANADQIASGIHF 73
S I++ + + ++Q N +S Y IG G +DITG +A+VN+MGYAN Q+ +GIHF
Sbjct: 20 SSNNIFIDSFKIPVNQKNVKSSGDSSYQIGAGIYDITGASAEVNLMGYANPLQVGAGIHF 79
Query: 74 RLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYG-DLYTEKNVAISGIHS 133
R RARAF+ + G R V+V+ D+CM Q V I V+E L+ +G ++YTE NV +SG H+
Sbjct: 80 RQRARAFVFVDSNGNRAVYVSTDSCMIFQEVKIHVVELLQDIFGPNVYTEANVLLSGTHT 139
Query: 134 HAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSILINKGELIDAGV 193
H+GP G+ QY +Y +TSLGF +++F+ + +GI ++I++AH+++ P ++ GEL + +
Sbjct: 140 HSGPAGFSQYALYGITSLGFYKKNFDTICNGIVQAIVKAHKSVQPANMFTETGELWNTNI 199
Query: 194 NRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALIS 253
NRSP AY NNP E++ Y +VDK MT+L+ D P ++FA H TSM+ TN LIS
Sbjct: 200 NRSPFAYDNNPEEEKAMYDSNVDKNMTVLRIEDMNGNPFAAISFFAVHCTSMNNTNHLIS 259
Query: 254 GDNKGAAARFMEDWFKQ-KGTGTLHPGESEADSIPRRVSNIVPEVYKDKQELLELAASFQ 313
GDNKG A+ W KQ G GT
Sbjct: 260 GDNKGYASYL---WEKQVNGPGT------------------------------------- 319
Query: 314 SQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFN 373
A + FV+AF QSN GDVSPNT G C D G PCD+
Sbjct: 320 -----------------------AGKGPFVAAFGQSNEGDVSPNTRGPTCRD-GSPCDYK 379
Query: 374 HSTCGGKNELCYGRGPGYP-DEFESTRIIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVD 433
STC G+NE C+ GPG D FEST+IIG QF KA++LF+ AS Q++G V +RHS+V
Sbjct: 380 TSTCNGRNEECWSLGPGKDGDMFESTQIIGGNQFNKALELFNNASIQVSGPVQYRHSWVQ 439
Query: 434 FSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDD--KGNAFWKLVRN 493
F+++ V GV TC AMG++FAAGTTDGPGAF+F Q D+ GN FW + +
Sbjct: 440 FTNVSVEPPYNSGVDNAT-TCRGAMGYSFAAGTTDGPGAFNFVQSDNNTSGNPFWNFIGD 499
Query: 494 VLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILNVPGEFTTMAG 553
+ P +QI CQSPKPILLD G M P W P ++PIQI+ IGQ+V++ VPGEFTTM+G
Sbjct: 500 FIAKPTPDQIRCQSPKPILLDVG-MVEPIPWVPDVMPIQIVTIGQIVLVAVPGEFTTMSG 559
Query: 554 RRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGASTLYGPHT 613
RRLR++V+ ++ E N V+IAGL+NTYS Y+ TFEEY++QRYEGAST++GPHT
Sbjct: 560 RRLRNSVREII-----GESIENPIVLIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHT 619
Query: 614 LEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSFGDVKFDVP 673
L +Y+QEF KLAQS++DGT V GP P +L + LPPVI+D P FG+V DV
Sbjct: 620 LGSYMQEFGKLAQSIVDGTTVPAGPTPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDV- 679
Query: 674 SNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQ-KNTWVPAYDDDDFCLRFKWSRP 733
N ++ V F+ G PRND M E +F V++L + W DD D+ +FKW
Sbjct: 680 -NLNYSVNETVSCVFYGGNPRNDFMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWKMH 717
Query: 734 APLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKS--LLGSIRHFTGSSSAFVV 779
S TIEW I G YRI H G AK ++ + G S FVV
Sbjct: 740 D--LGFSLITIEWVIAPDTTPGTYRITHSGFAKKNPFSSNLTPYQGISRNFVV 717
BLAST of CSPI01G23580 vs. ExPASy TrEMBL
Match:
A0A0A0LY63 (Neutral ceramidase OS=Cucumis sativus OX=3659 GN=Csa_1G527930 PE=3 SV=1)
HSP 1 Score: 1566.6 bits (4055), Expect = 0.0e+00
Identity = 776/779 (99.61%), Postives = 778/779 (99.87%), Query Frame = 0
Query: 1 MELSSLFNLTVGRSLGTIWLCIYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
MELSSLFNLTVGRSLGTIWLCIYMLVLS+SNRSVLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MELSSLFNLTVGRSLGTIWLCIYMLVLSESNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANADQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YANADQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANADQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSI 180
TEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSI
Sbjct: 121 TEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
LINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH
Sbjct: 181 LINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
Query: 241 GTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHPGESEADSIPRRVSNIVPEVYKDK 300
GTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLH GESEADSIPRRVSNIVPEVYKDK
Sbjct: 241 GTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSNIVPEVYKDK 300
Query: 301 QELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTG 420
CLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTG
Sbjct: 361 CLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTG 420
Query: 421 KVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
KVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 KVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILNVP 540
AFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVIL+VP
Sbjct: 481 AFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILDVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA 600
Query: 601 STLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSF 660
STLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSF
Sbjct: 601 STLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSF 660
Query: 661 GDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVPAYDDDDFCL 720
GDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVPAYDDDDFCL
Sbjct: 661 GDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVPAYDDDDFCL 720
Query: 721 RFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA 780
RFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA
Sbjct: 721 RFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA 779
BLAST of CSPI01G23580 vs. ExPASy TrEMBL
Match:
A0A1S3C5F7 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103497106 PE=3 SV=1)
HSP 1 Score: 1509.2 bits (3906), Expect = 0.0e+00
Identity = 743/778 (95.50%), Postives = 755/778 (97.04%), Query Frame = 0
Query: 1 MELSSLFNLTVGRSLGTIWLCIYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
MELSSLFNLTV RSLGTIWLCIYMLVLSQS RSVLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MELSSLFNLTVRRSLGTIWLCIYMLVLSQSTRSVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANADQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YANA+QIASG+HFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANAEQIASGVHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSI 180
TEKNVAISGIHSHAGPGGYLQY+VYIVTSLGFVRQSF+VLVDGIEKSIIQAHENLSPGSI
Sbjct: 121 TEKNVAISGIHSHAGPGGYLQYIVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLSPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
LINKGELIDAGVNRSPSAYLNNPASE SKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH
Sbjct: 181 LINKGELIDAGVNRSPSAYLNNPASECSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
Query: 241 GTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHPGESEADSIPRRVSNIVPEVYKDK 300
GTSMSRTN+LISGDNKGAAARFMEDWF+QKGTGTLHPGESEADSIPRRVSNI+PEVYKDK
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGTLHPGESEADSIPRRVSNIIPEVYKDK 300
Query: 301 QELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELLE AASFQSQPGRPATR LS+SSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLERAASFQSQPGRPATRALSVSSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTG 420
CLDT LPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGE+QFRKAVDLF KASE LTG
Sbjct: 361 CLDTELPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFGKASEHLTG 420
Query: 421 KVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
KVDFRHSYVDFSHLEVSL KQGG EVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 KVDFRHSYVDFSHLEVSLTKQGGAAEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILNVP 540
FWKLVRN+LKAPGNEQISCQSPKPILLDTGEMK PYDWAP+ILPIQILRIGQLVIL+VP
Sbjct: 481 PFWKLVRNLLKAPGNEQISCQSPKPILLDTGEMKIPYDWAPTILPIQILRIGQLVILSVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGA KEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGANKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA 600
Query: 601 STLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSF 660
STLYGPHTLEAYIQEFKKLAQSLIDG PV PGPQPPDLL RQISLLPPVILDMTP GVSF
Sbjct: 601 STLYGPHTLEAYIQEFKKLAQSLIDGRPVVPGPQPPDLLDRQISLLPPVILDMTPPGVSF 660
Query: 661 GDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVPAYDDDDFCL 720
GDVK DVP NSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVPAYDDDDFCL
Sbjct: 661 GDVKSDVPRNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVPAYDDDDFCL 720
Query: 721 RFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 779
RFKWSRPAPLSAQSYATIEWRIPQT VSGVYRIRHFGAAKS GSIRHFTGSSSAFVV
Sbjct: 721 RFKWSRPAPLSAQSYATIEWRIPQTVVSGVYRIRHFGAAKSFFGSIRHFTGSSSAFVV 778
BLAST of CSPI01G23580 vs. ExPASy TrEMBL
Match:
A0A6J1HIT9 (Neutral ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111464833 PE=3 SV=1)
HSP 1 Score: 1431.4 bits (3704), Expect = 0.0e+00
Identity = 699/780 (89.62%), Postives = 742/780 (95.13%), Query Frame = 0
Query: 1 MELSSLFNLTVGRSLGTIWLCIYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
M LSSLF+ TV RSLG+IWLC+YMLV+ QSNR VLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MGLSSLFDRTVRRSLGSIWLCVYMLVILQSNRRVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANADQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN +QIASG+HFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANTEQIASGVHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSI 180
TE+NVAISGIH+HAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQAHENL PGSI
Sbjct: 121 TEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
L+NKGELIDAGVNRSPSAYLNNPASER++YKYDVDKEMTLLKFIDD+WGPVG+FNWFATH
Sbjct: 181 LVNKGELIDAGVNRSPSAYLNNPASERNRYKYDVDKEMTLLKFIDDQWGPVGSFNWFATH 240
Query: 241 GTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHPGESEADSIPRRVSNIVPEVYKDK 300
GTSMSRTN+LISGDNKGAAARFMEDWF QKG GTL G+SEADSIPRRVSNI+PE Y D+
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFVQKGIGTLQTGKSEADSIPRRVSNIIPEAYNDE 300
Query: 301 QELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELL AASFQS+PGRPATR+LS+S RVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLARAASFQSRPGRPATRILSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTG 420
C+DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGE+QFRKAVDLFSKASEQL G
Sbjct: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLKG 420
Query: 421 KVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
VD+RHSYVDFS LEVS+ K GG TEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 TVDYRHSYVDFSQLEVSVTKAGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILNVP 540
AFWKLVRN+LK PG++QI+CQSPKPILLDTGEMK PYDWAPSILPIQILRIGQLVIL VP
Sbjct: 481 AFWKLVRNLLKTPGSKQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGAK+EFN NVHVVIAGLTNTYSQYVTTFEEY+MQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGA 600
Query: 601 STLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSF 660
STLYGPHTL+AYIQEF+KLAQSLIDG PV PGPQPPDLLA+QISLL PV++D TP GVSF
Sbjct: 601 STLYGPHTLDAYIQEFRKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVMDATPFGVSF 660
Query: 661 GDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQ-KNTWVPAYDDDDFC 720
GDV+ DVP NS+FKRG+LV VTFW+GCPRNDLMTEGTFALVEILQ K+TWVP+YDDDDFC
Sbjct: 661 GDVRSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPSYDDDDFC 720
Query: 721 LRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA 780
LRFKWSRP+ LS QS ATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA
Sbjct: 721 LRFKWSRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA 780
BLAST of CSPI01G23580 vs. ExPASy TrEMBL
Match:
A0A6J1DAR9 (Neutral ceramidase OS=Momordica charantia OX=3673 GN=LOC111018960 PE=3 SV=1)
HSP 1 Score: 1421.8 bits (3679), Expect = 0.0e+00
Identity = 695/780 (89.10%), Postives = 737/780 (94.49%), Query Frame = 0
Query: 1 MELSSLFNLTVGRSLGTIWLCIYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
M LSSL +LTVGRSL T+WL I MLVL QSN VLSESKYLIGLGS+DITGPAADVNMMG
Sbjct: 1 MGLSSLVDLTVGRSLATLWLWICMLVLLQSNGRVLSESKYLIGLGSYDITGPAADVNMMG 60
Query: 61 YANADQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN +QIASG+HFRLRARAFIVAEPQG RVVFVNLDACMASQ+VTIKVLERLKARYGDLY
Sbjct: 61 YANIEQIASGVHFRLRARAFIVAEPQGNRVVFVNLDACMASQLVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSI 180
TEKNVAISGIH+HAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQAHENL PGS+
Sbjct: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSV 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
LIN GELIDAGVNRSPSAYLNNPASERS+YKYDVDKEMTLLKF+DDEWGPVG+FNWFATH
Sbjct: 181 LINTGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
Query: 241 GTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHPGESEADSIPRRVSNIVPEVYKDK 300
GTSMSRTN+LISGDNKGAAARFMEDWF QKG G+LHPG+S ADSIPRRVSNI+PE YK+K
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFDQKGIGSLHPGQSVADSIPRRVSNIIPEAYKNK 300
Query: 301 QELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELLE AASFQS+PGRPATR+LS++ RVRNVLRQADRPQFVSAFCQSNCGDVSPN LGAF
Sbjct: 301 QELLERAASFQSRPGRPATRILSVARRVRNVLRQADRPQFVSAFCQSNCGDVSPNVLGAF 360
Query: 361 CLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTG 420
C+DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFES RIIGE+QFRKAVDLF+KASE+LTG
Sbjct: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESARIIGERQFRKAVDLFNKASEKLTG 420
Query: 421 KVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
KVD+RHSYVDFS LEVSL KQGG TEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 KVDYRHSYVDFSQLEVSLLKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILNVP 540
FWKLVRN+L+APG +Q++CQSPKPILLDTGEMK PYDWAPSILPIQILRIGQLVIL VP
Sbjct: 481 PFWKLVRNLLRAPGEQQVTCQSPKPILLDTGEMKAPYDWAPSILPIQILRIGQLVILGVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA 600
GEFTTMAGRRLR+AVKTVLTTGAK+EFN NVH+VIAGLTNTYSQYVTTFEEY+MQRYEGA
Sbjct: 541 GEFTTMAGRRLRNAVKTVLTTGAKREFNGNVHIVIAGLTNTYSQYVTTFEEYEMQRYEGA 600
Query: 601 STLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSF 660
STLYGPHTL+AYIQEFKKLAQSLIDG PV PGPQPPDLL+RQISLLPPVI+D TPLGVSF
Sbjct: 601 STLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLSRQISLLPPVIMDATPLGVSF 660
Query: 661 GDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQ-KNTWVPAYDDDDFC 720
GDVK DVP NS+FKRGNLV VTFW+GCPRNDLMTEGTFALVEILQ K+TWVP YDDDDFC
Sbjct: 661 GDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPVYDDDDFC 720
Query: 721 LRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA 780
L FKW RP S QSYATIEWRIPQ+AVSGVYRIRHFGAAKSL GSIRHFTGSSSAFVVA
Sbjct: 721 LFFKWLRPFRYSTQSYATIEWRIPQSAVSGVYRIRHFGAAKSLFGSIRHFTGSSSAFVVA 780
BLAST of CSPI01G23580 vs. ExPASy TrEMBL
Match:
A0A6J1I331 (Neutral ceramidase OS=Cucurbita maxima OX=3661 GN=LOC111469470 PE=3 SV=1)
HSP 1 Score: 1419.8 bits (3674), Expect = 0.0e+00
Identity = 694/780 (88.97%), Postives = 737/780 (94.49%), Query Frame = 0
Query: 1 MELSSLFNLTVGRSLGTIWLCIYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
M LSSLF+ TV RSLG+IWLC+YMLV+ QSNR VLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MGLSSLFDRTVRRSLGSIWLCVYMLVILQSNRRVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANADQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN +QIASG+HFRLRARAFIVAEPQGK+VVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANTEQIASGVHFRLRARAFIVAEPQGKQVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSI 180
TE+NVAISGIH+HAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQAHENL PGSI
Sbjct: 121 TEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
L+NKGELIDA VNRSPSAYLNNPASER +YKYDVDKEMTLLKF+DD+WGPVG+FNWFATH
Sbjct: 181 LVNKGELIDASVNRSPSAYLNNPASERDRYKYDVDKEMTLLKFVDDQWGPVGSFNWFATH 240
Query: 241 GTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHPGESEADSIPRRVSNIVPEVYKDK 300
GTSMSRTN+LISGDNKGAAARFMEDWF QKG TL G+SEADSIPRRVSNI+PE YKD+
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFVQKGIRTLQTGKSEADSIPRRVSNIIPEAYKDE 300
Query: 301 QELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELLE AA+FQS+PGRPATR+LS+S RVRN LRQADRPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLERAAAFQSRPGRPATRILSVSRRVRNALRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTG 420
C+DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGE+QFRKAVDLFSKASEQL G
Sbjct: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLKG 420
Query: 421 KVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
VD+RHSYVDFS LEVS+ K GG T VVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 TVDYRHSYVDFSQLEVSVTKPGGATAVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILNVP 540
AFWKLVRN+LK PG+EQI+CQSPKPILLDTGEMK PYDWAPSILPIQILRIGQLVIL VP
Sbjct: 481 AFWKLVRNLLKTPGSEQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGAK+EFN NVHVVIAGLTNTYSQYVTTFEEY+MQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGA 600
Query: 601 STLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSF 660
STLYGPHTL+AYIQEFKKLAQSLIDG PV PGPQPPDLLA+QISLL PV++D TP GVSF
Sbjct: 601 STLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVMDATPFGVSF 660
Query: 661 GDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQ-KNTWVPAYDDDDFC 720
GDVK DVP NS+FKRG+LV VTFW+GCPRNDLMTEGTFALVEILQ K+TWVP+YDDDDFC
Sbjct: 661 GDVKSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPSYDDDDFC 720
Query: 721 LRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA 780
LRFKW RP+ LS QS ATIEWRIPQTAVSGVYRIRHFGAAK+LL SIRHFTGSSSAFVVA
Sbjct: 721 LRFKWWRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKNLLRSIRHFTGSSSAFVVA 780
BLAST of CSPI01G23580 vs. NCBI nr
Match:
XP_004153679.2 (neutral ceramidase 2 [Cucumis sativus] >KGN65777.1 hypothetical protein Csa_023365 [Cucumis sativus])
HSP 1 Score: 1566.6 bits (4055), Expect = 0.0e+00
Identity = 776/779 (99.61%), Postives = 778/779 (99.87%), Query Frame = 0
Query: 1 MELSSLFNLTVGRSLGTIWLCIYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
MELSSLFNLTVGRSLGTIWLCIYMLVLS+SNRSVLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MELSSLFNLTVGRSLGTIWLCIYMLVLSESNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANADQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YANADQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANADQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSI 180
TEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSI
Sbjct: 121 TEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
LINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH
Sbjct: 181 LINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
Query: 241 GTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHPGESEADSIPRRVSNIVPEVYKDK 300
GTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLH GESEADSIPRRVSNIVPEVYKDK
Sbjct: 241 GTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSNIVPEVYKDK 300
Query: 301 QELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTG 420
CLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTG
Sbjct: 361 CLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTG 420
Query: 421 KVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
KVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 KVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILNVP 540
AFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVIL+VP
Sbjct: 481 AFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILDVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA 600
Query: 601 STLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSF 660
STLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSF
Sbjct: 601 STLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSF 660
Query: 661 GDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVPAYDDDDFCL 720
GDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVPAYDDDDFCL
Sbjct: 661 GDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVPAYDDDDFCL 720
Query: 721 RFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA 780
RFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA
Sbjct: 721 RFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA 779
BLAST of CSPI01G23580 vs. NCBI nr
Match:
XP_008457412.1 (PREDICTED: neutral ceramidase-like isoform X1 [Cucumis melo])
HSP 1 Score: 1509.2 bits (3906), Expect = 0.0e+00
Identity = 743/778 (95.50%), Postives = 755/778 (97.04%), Query Frame = 0
Query: 1 MELSSLFNLTVGRSLGTIWLCIYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
MELSSLFNLTV RSLGTIWLCIYMLVLSQS RSVLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MELSSLFNLTVRRSLGTIWLCIYMLVLSQSTRSVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANADQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YANA+QIASG+HFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANAEQIASGVHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSI 180
TEKNVAISGIHSHAGPGGYLQY+VYIVTSLGFVRQSF+VLVDGIEKSIIQAHENLSPGSI
Sbjct: 121 TEKNVAISGIHSHAGPGGYLQYIVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLSPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
LINKGELIDAGVNRSPSAYLNNPASE SKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH
Sbjct: 181 LINKGELIDAGVNRSPSAYLNNPASECSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
Query: 241 GTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHPGESEADSIPRRVSNIVPEVYKDK 300
GTSMSRTN+LISGDNKGAAARFMEDWF+QKGTGTLHPGESEADSIPRRVSNI+PEVYKDK
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGTLHPGESEADSIPRRVSNIIPEVYKDK 300
Query: 301 QELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELLE AASFQSQPGRPATR LS+SSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLERAASFQSQPGRPATRALSVSSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTG 420
CLDT LPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGE+QFRKAVDLF KASE LTG
Sbjct: 361 CLDTELPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFGKASEHLTG 420
Query: 421 KVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
KVDFRHSYVDFSHLEVSL KQGG EVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 KVDFRHSYVDFSHLEVSLTKQGGAAEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILNVP 540
FWKLVRN+LKAPGNEQISCQSPKPILLDTGEMK PYDWAP+ILPIQILRIGQLVIL+VP
Sbjct: 481 PFWKLVRNLLKAPGNEQISCQSPKPILLDTGEMKIPYDWAPTILPIQILRIGQLVILSVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGA KEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGANKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA 600
Query: 601 STLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSF 660
STLYGPHTLEAYIQEFKKLAQSLIDG PV PGPQPPDLL RQISLLPPVILDMTP GVSF
Sbjct: 601 STLYGPHTLEAYIQEFKKLAQSLIDGRPVVPGPQPPDLLDRQISLLPPVILDMTPPGVSF 660
Query: 661 GDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVPAYDDDDFCL 720
GDVK DVP NSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVPAYDDDDFCL
Sbjct: 661 GDVKSDVPRNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVPAYDDDDFCL 720
Query: 721 RFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 779
RFKWSRPAPLSAQSYATIEWRIPQT VSGVYRIRHFGAAKS GSIRHFTGSSSAFVV
Sbjct: 721 RFKWSRPAPLSAQSYATIEWRIPQTVVSGVYRIRHFGAAKSFFGSIRHFTGSSSAFVV 778
BLAST of CSPI01G23580 vs. NCBI nr
Match:
XP_038892835.1 (neutral ceramidase 2-like [Benincasa hispida])
HSP 1 Score: 1480.7 bits (3832), Expect = 0.0e+00
Identity = 724/780 (92.82%), Postives = 759/780 (97.31%), Query Frame = 0
Query: 1 MELSSLFNLTVGRSLGTIWLCIYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
MELSSLF+LTV RSLGTIWLCIYMLVL QSNRSVLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MELSSLFDLTVRRSLGTIWLCIYMLVLLQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANADQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YANA+QIASG+HFRLRARAFIVAEPQGKRV+FVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANAEQIASGVHFRLRARAFIVAEPQGKRVIFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSI 180
TEKNVAISGIH+HAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQAHENL PGSI
Sbjct: 121 TEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
LINKGELIDAGVNRSPSAYLNNPASERS+YKYDVDKEMTLLKF+DDEWGPVG+FNWFATH
Sbjct: 181 LINKGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPVGSFNWFATH 240
Query: 241 GTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHPGESEADSIPRRVSNIVPEVYKDK 300
GTSMSRTN+LISGDNKGAAARFMEDWF+QKGTGT+HPGESE DSIPRRVSNI+PEV+K+K
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGTMHPGESEVDSIPRRVSNIIPEVFKNK 300
Query: 301 QELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELLE AASFQSQPGRPATR+LS+S RVRNVLRQA+RPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLERAASFQSQPGRPATRILSVSRRVRNVLRQANRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTG 420
C+DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGE+QFRKAVDLFSKASEQLTG
Sbjct: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLTG 420
Query: 421 KVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
KVDFRHSYVDFS LEVSL+KQGG TEV++TCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 KVDFRHSYVDFSQLEVSLSKQGGATEVIRTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILNVP 540
AFWKLVRN+LKAPGNEQI+CQSPKPILLDTGEMK PYDWAPSILPIQILRIGQLVIL VP
Sbjct: 481 AFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGAK+EFN NVHVVIAGLTNTYSQYVTTFEEY+MQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGA 600
Query: 601 STLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSF 660
STLYGPHTL+AYIQEFKKLAQSLIDG PV PGPQPPDLLARQISLL PVI+D TP+GVSF
Sbjct: 601 STLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIMDATPIGVSF 660
Query: 661 GDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQ-KNTWVPAYDDDDFC 720
GDVK DVP NS+FKRGNLV V+FW+GCPRNDLMTEGTFALVEILQ K+TWVPAYDDDDFC
Sbjct: 661 GDVKSDVPLNSTFKRGNLVTVSFWSGCPRNDLMTEGTFALVEILQKKDTWVPAYDDDDFC 720
Query: 721 LRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA 780
LRFKWSRP+ LS QSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA
Sbjct: 721 LRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA 780
BLAST of CSPI01G23580 vs. NCBI nr
Match:
KAG6583392.1 (Neutral ceramidase 1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1434.1 bits (3711), Expect = 0.0e+00
Identity = 701/780 (89.87%), Postives = 743/780 (95.26%), Query Frame = 0
Query: 1 MELSSLFNLTVGRSLGTIWLCIYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
M LSSLF+ TV RSLG+IWLC+YMLV+ QSNR VLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MGLSSLFDHTVRRSLGSIWLCVYMLVILQSNRRVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANADQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN +QIASG+HFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANTEQIASGVHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSI 180
TE+NVAISGIH+HAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQAHENL PGSI
Sbjct: 121 TEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
L+NKGELIDAGVNRSPSAYLNNPASER++YKYDVDKEMTLLKFIDD+WGPVG+FNWFATH
Sbjct: 181 LVNKGELIDAGVNRSPSAYLNNPASERNRYKYDVDKEMTLLKFIDDQWGPVGSFNWFATH 240
Query: 241 GTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHPGESEADSIPRRVSNIVPEVYKDK 300
GTSMSRTN+LISGDNKGAAARFMEDWF QKG GTL G+SEADSIPRRVSNI+PE YKD+
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFVQKGIGTLQTGKSEADSIPRRVSNIIPEAYKDE 300
Query: 301 QELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELL AASFQS+PGRPATR+LS+S RVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLARAASFQSRPGRPATRILSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTG 420
C+DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGE+QFRKAVDLFSKASEQL G
Sbjct: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLKG 420
Query: 421 KVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
VD+RHSYVDFS LEVS+ K GG TEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 TVDYRHSYVDFSQLEVSVTKAGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILNVP 540
AFWKLVRN+LK PG++QI+CQSPKPILLDTGEMK PYDWAPSILPIQILRIGQLVIL VP
Sbjct: 481 AFWKLVRNLLKTPGSKQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGAK+EFN NVHVVIAGLTNTYSQYVTTFEEY+MQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGA 600
Query: 601 STLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSF 660
STLYGPHTL+AYIQEF+KLAQSLIDG PV PGPQPPDLLA+QISLL PV++D TP GVSF
Sbjct: 601 STLYGPHTLDAYIQEFRKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVMDATPFGVSF 660
Query: 661 GDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQ-KNTWVPAYDDDDFC 720
GDVK DVP NS+FKRG+LV VTFW+GCPRNDLMTEGTFALVEILQ K+TWVP+YDDDDFC
Sbjct: 661 GDVKSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPSYDDDDFC 720
Query: 721 LRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA 780
LRFKWSRP+ LS QS ATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA
Sbjct: 721 LRFKWSRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA 780
BLAST of CSPI01G23580 vs. NCBI nr
Match:
KAG7019159.1 (Neutral ceramidase 1 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1433.3 bits (3709), Expect = 0.0e+00
Identity = 700/780 (89.74%), Postives = 743/780 (95.26%), Query Frame = 0
Query: 1 MELSSLFNLTVGRSLGTIWLCIYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMG 60
M LSSLF+ TV RSLG+IWLC+YMLV+ QSNR VLSESKYLIGLGSHDITGPAADVNMMG
Sbjct: 1 MGLSSLFDRTVRRSLGSIWLCVYMLVILQSNRRVLSESKYLIGLGSHDITGPAADVNMMG 60
Query: 61 YANADQIASGIHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
YAN +QIASG+HFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY
Sbjct: 61 YANTEQIASGVHFRLRARAFIVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLY 120
Query: 121 TEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSI 180
TE+NVAISGIH+HAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQAHENL PGSI
Sbjct: 121 TEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQAHENLLPGSI 180
Query: 181 LINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATH 240
L+NKGELIDAGVNRSPSAYLNNPASER++YKYDVDKEMTLLKFIDD+WGPVG+FNWFATH
Sbjct: 181 LVNKGELIDAGVNRSPSAYLNNPASERNRYKYDVDKEMTLLKFIDDQWGPVGSFNWFATH 240
Query: 241 GTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHPGESEADSIPRRVSNIVPEVYKDK 300
GTSMSRTN+LISGDNKGAAARFMEDWF QKG GTL G+SEADSIPRRVSNI+PE Y+D+
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFVQKGIGTLQTGKSEADSIPRRVSNIIPEAYRDE 300
Query: 301 QELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
QELL AASFQS+PGRPATR+LS+S RVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF
Sbjct: 301 QELLARAASFQSRPGRPATRILSVSRRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAF 360
Query: 361 CLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTG 420
C+DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGE+QFRKAVDLFSKASEQL G
Sbjct: 361 CIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLFSKASEQLKG 420
Query: 421 KVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
VD+RHSYVDFS LEVS+ K GG TEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN
Sbjct: 421 TVDYRHSYVDFSQLEVSVTKAGGATEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGN 480
Query: 481 AFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILNVP 540
AFWKLVRN+LK PG++QI+CQSPKPILLDTGEMK PYDWAPSILPIQILRIGQLVIL VP
Sbjct: 481 AFWKLVRNLLKTPGSKQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRIGQLVILGVP 540
Query: 541 GEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGA 600
GEFTTMAGRRLRDAVKTVLTTGAK+EFN NVHVVIAGLTNTYSQYVTTFEEY+MQRYEGA
Sbjct: 541 GEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEEYEMQRYEGA 600
Query: 601 STLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSF 660
STLYGPHTL+AYIQEF+KLAQSLIDG PV PGPQPPDLLA+QISLL PV++D TP GVSF
Sbjct: 601 STLYGPHTLDAYIQEFRKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVMDATPFGVSF 660
Query: 661 GDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQ-KNTWVPAYDDDDFC 720
GDVK DVP NS+FKRG+LV VTFW+GCPRNDLMTEGTFALVEILQ K+TWVP+YDDDDFC
Sbjct: 661 GDVKSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWVPSYDDDDFC 720
Query: 721 LRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA 780
LRFKWSRP+ LS QS ATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA
Sbjct: 721 LRFKWSRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA 780
BLAST of CSPI01G23580 vs. TAIR 10
Match:
AT1G07380.1 (Neutral/alkaline non-lysosomal ceramidase )
HSP 1 Score: 1163.3 bits (3008), Expect = 0.0e+00
Identity = 563/748 (75.27%), Postives = 636/748 (85.03%), Query Frame = 0
Query: 34 VLSESKYLIGLGSHDITGPAADVNMMGYANADQIASGIHFRLRARAFIVAEPQGKRVVFV 93
V S S+YLIGLGS+DITGPAADVNMMGYAN +Q+ASGIHFRLRAR FIV+EPQGKRVVFV
Sbjct: 31 VYSHSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSEPQGKRVVFV 90
Query: 94 NLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFV 153
NLDACMASQIV +KV+ERLKARYGDLYTE+NV ISGIH+HAGPGGYLQYVVYIVTSLGFV
Sbjct: 91 NLDACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFV 150
Query: 154 RQSFNVLVDGIEKSIIQAHENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYD 213
RQSF+ LVDGIE SIIQAHENL PGSI +N GEL+DAGVNRSPSAYLNNP+ ERSK+KY+
Sbjct: 151 RQSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYN 210
Query: 214 VDKEMTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTG 273
VDKEMTLLKF+DD+WGPVG+FNWFATHGTSMSRTN+LISGDNKGAA+RFMEDW++Q
Sbjct: 211 VDKEMTLLKFVDDQWGPVGSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAE 270
Query: 274 TLHPGESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLR 333
+ E +D IPRRVS+++ ELLELA+ F+SQPG+P TR+ S + RVR+ LR
Sbjct: 271 RSYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRISSSARRVRSALR 330
Query: 334 QADRPQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEF 393
+AD+P FVSAFCQ+NCGDVSPN LGAFCLDTGLPCDFNHSTCGGKNE+CYGRGPGYPDEF
Sbjct: 331 KADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEF 390
Query: 394 ESTRIIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPA 453
ESTRIIGE+QF+ A++LF+KASEQL GKVD+RH YVDFS L V+L K+ G +EVVKTCPA
Sbjct: 391 ESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPA 450
Query: 454 AMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEM 513
AMGFAFAAGTTDGPGAFDF QGDDKGN FW+LVRNVLK P +QI C PKPILLDTGEM
Sbjct: 451 AMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEM 510
Query: 514 KTPYDWAPSILPIQILRIGQLVILNVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHV 573
PYDWAPSIL +Q+LRIGQL IL+VPGEFTTMAGRRLR AVKT L K+ + +HV
Sbjct: 511 TKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHV 570
Query: 574 VIAGLTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGP 633
VIAGL N YSQYVTTFEEY++QRYEGASTLYGPHTL YIQEFKKL++SL+ PV PGP
Sbjct: 571 VIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLVLDMPVQPGP 630
Query: 634 QPPDLLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGN-LVKVTFWTGCPRNDL 693
QPPDLL +Q+S L PV++D TP G SFGDV DVP N S KRGN V V F + CPRNDL
Sbjct: 631 QPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDVPKNLSLKRGNGQVTVVFRSACPRNDL 690
Query: 694 MTEGTFALVEILQK--NTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGV 753
+TEGTF LVE L++ TW P YDDDD CLRFKWSR LS++S AT+EWRIP++A GV
Sbjct: 691 LTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESASPGV 750
Query: 754 YRIRHFGAAKSLLGSIRHFTGSSSAFVV 779
YRI HFGAAK L GS+ HFTGSSSAFVV
Sbjct: 751 YRITHFGAAKKLFGSVHHFTGSSSAFVV 778
BLAST of CSPI01G23580 vs. TAIR 10
Match:
AT2G38010.1 (Neutral/alkaline non-lysosomal ceramidase )
HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 556/758 (73.35%), Postives = 655/758 (86.41%), Query Frame = 0
Query: 22 IYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMGYANADQIASGIHFRLRARAFI 81
I L+L +R+V + YLIG+GS+DITGPAADVNMMGYAN+DQIASGIHFRLRARAFI
Sbjct: 11 ILFLLLLLLSRTVYA---YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFI 70
Query: 82 VAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHSHAGPGGYLQ 141
VAEPQG RVVFVNLDACMASQIVTIKVLERLKARYG+LYTEKNVAISGIH+HAGPGGYLQ
Sbjct: 71 VAEPQGNRVVFVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQ 130
Query: 142 YVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSILINKGELIDAGVNRSPSAYLN 201
YV YIVTSLGFVRQSF+V+V+GIE+SI+QAHE+L PGS +NKG+L+DAGVNRSPS+YLN
Sbjct: 131 YVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLN 190
Query: 202 NPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAAR 261
NPA+ERSKYKYDVDKEMTL+KF+D + GP G+FNWFATHGTSMSRTN+LISGDNKGAAAR
Sbjct: 191 NPAAERSKYKYDVDKEMTLVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGAAAR 250
Query: 262 FMEDWFKQKGTGTLHPGESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRV 321
FMEDWF+ ++ + +IPRRVS IV + +++ LL++AA+++S G +
Sbjct: 251 FMEDWFENGQKNSV-----SSRNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKS 310
Query: 322 LSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNEL 381
L + +RVRN + +FVSAFCQSNCGDVSPNTLG FC+DTGLPCDFNHSTC G+NEL
Sbjct: 311 LDVKTRVRN----GSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNEL 370
Query: 382 CYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQ 441
CYGRGPGYPDEFESTRIIGEKQF+ AV+LF+KA+E+L GK+ ++H+Y+DFS+L+V++ K
Sbjct: 371 CYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKA 430
Query: 442 GGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQ 501
GG +E VKTCPAAMGF FAAGTTDGPGAFDFKQGDD+GN FW+LVRNVL+ PG EQ+ CQ
Sbjct: 431 GGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQ 490
Query: 502 SPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILNVPGEFTTMAGRRLRDAVKTVLTT 561
PKPILLDTGEMK PYDWAPSILPIQILRIGQLVIL+VPGEFTTMAGRRLRDA+K+ L +
Sbjct: 491 KPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLIS 550
Query: 562 GAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQ 621
KEF++N+HVVIAGLTNTYSQY+ TFEEY++QRYEGASTLYG HTL AYIQEFKKLA
Sbjct: 551 SDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLAT 610
Query: 622 SLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKV 681
+L++G + GPQPPDLL +QISLL PV++D TPLGV FGDVK DVP S+F+RG V
Sbjct: 611 ALVNGLTLPRGPQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADVPPKSTFRRGQQVNA 670
Query: 682 TFWTGCPRNDLMTEGTFALVEILQK-NTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEW 741
TFW+GCPRNDLMTEG+FA+VE L++ W P YDDDDF L+FKWSRPA LS++S ATIEW
Sbjct: 671 TFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEW 730
Query: 742 RIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 779
R+P++AV+GVYRIRH+GA+KSL GSI F+GSSSAFVV
Sbjct: 731 RVPESAVAGVYRIRHYGASKSLFGSISSFSGSSSAFVV 756
BLAST of CSPI01G23580 vs. TAIR 10
Match:
AT2G38010.2 (Neutral/alkaline non-lysosomal ceramidase )
HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 556/793 (70.11%), Postives = 656/793 (82.72%), Query Frame = 0
Query: 22 IYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMGYANADQIASGIHFRLRARAFI 81
I L+L +R+V + YLIG+GS+DITGPAADVNMMGYAN+DQIASGIHFRLRARAFI
Sbjct: 11 ILFLLLLLLSRTVYA---YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFI 70
Query: 82 VAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHSHAGPGGYLQ 141
VAEPQG RVVFVNLDACMASQIVTIKVLERLKARYG+LYTEKNVAISGIH+HAGPGGYLQ
Sbjct: 71 VAEPQGNRVVFVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQ 130
Query: 142 YVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSILINKGELIDAGVNRSPSAYLN 201
YV YIVTSLGFVRQSF+V+V+GIE+SI+QAHE+L PGS +NKG+L+DAGVNRSPS+YLN
Sbjct: 131 YVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLN 190
Query: 202 NPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAAR 261
NPA+ERSKYKYDVDKEMTL+KF+D + GP G+FNWFATHGTSMSRTN+LISGDNKGAAAR
Sbjct: 191 NPAAERSKYKYDVDKEMTLVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGAAAR 250
Query: 262 FMEDWFKQKGTGTLHPGESEADSIPRRVSNIVPEVYKDKQ-------------------- 321
FMEDWF+ ++ + +IPRRVS IV + ++++
Sbjct: 251 FMEDWFENGQKNSV-----SSRNIPRRVSTIVSDFSRNRESYPFLELPNVAILYIAVAER 310
Query: 322 ---------------ELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQ 381
LL++AA+++S G + L + +RVRN + +FVSAFCQ
Sbjct: 311 LMWKVMVLFYFVSESRLLDIAATYKSSRGHSVDKSLDVKTRVRN----GSKRKFVSAFCQ 370
Query: 382 SNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRK 441
SNCGDVSPNTLG FC+DTGLPCDFNHSTC G+NELCYGRGPGYPDEFESTRIIGEKQF+
Sbjct: 371 SNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKM 430
Query: 442 AVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDG 501
AV+LF+KA+E+L GK+ ++H+Y+DFS+L+V++ K GG +E VKTCPAAMGF FAAGTTDG
Sbjct: 431 AVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDG 490
Query: 502 PGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPI 561
PGAFDFKQGDD+GN FW+LVRNVL+ PG EQ+ CQ PKPILLDTGEMK PYDWAPSILPI
Sbjct: 491 PGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPI 550
Query: 562 QILRIGQLVILNVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYV 621
QILRIGQLVIL+VPGEFTTMAGRRLRDA+K+ L + KEF++N+HVVIAGLTNTYSQY+
Sbjct: 551 QILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYI 610
Query: 622 TTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLL 681
TFEEY++QRYEGASTLYG HTL AYIQEFKKLA +L++G + GPQPPDLL +QISLL
Sbjct: 611 ATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPRGPQPPDLLDKQISLL 670
Query: 682 PPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQK 741
PV++D TPLGV FGDVK DVP S+F+RG V TFW+GCPRNDLMTEG+FA+VE L++
Sbjct: 671 SPVVVDSTPLGVKFGDVKADVPPKSTFRRGQQVNATFWSGCPRNDLMTEGSFAVVETLRE 730
Query: 742 -NTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGS 779
W P YDDDDF L+FKWSRPA LS++S ATIEWR+P++AV+GVYRIRH+GA+KSL GS
Sbjct: 731 GGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASKSLFGS 790
BLAST of CSPI01G23580 vs. TAIR 10
Match:
AT5G58980.1 (Neutral/alkaline non-lysosomal ceramidase )
HSP 1 Score: 1064.3 bits (2751), Expect = 4.7e-311
Identity = 531/762 (69.69%), Postives = 614/762 (80.58%), Query Frame = 0
Query: 21 CIYMLVLSQSNRSVLSESKYLIGLGSHDITGPAADVNMMGYANADQIASGIHFRLRARAF 80
C L+ + S+S YL+GLGS+DITGPAADVNMMGYAN +Q+ASG+HFRLRARAF
Sbjct: 10 CTLFLLFLLRLTCIFSDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAF 69
Query: 81 IVAEPQGKRVVFVNLDACMASQIVTIKVLERLKARYGDLYTEKNVAISGIHSHAGPGGYL 140
IVAEP KR+ FVNLDA MASQ+VTIKV+ERLK RYG+LYTE+NVAISG H+HAGPGGYL
Sbjct: 70 IVAEPYKKRIAFVNLDAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYL 129
Query: 141 QYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSPGSILINKGELIDAGVNRSPSAYL 200
QY++Y+VTSLGFV QSFN LVDGIE+SIIQAHENL PGSILINKGEL+DAGVNRSPSAYL
Sbjct: 130 QYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYL 189
Query: 201 NNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAA 260
NNPA ERSKY+YDVDKEMTL+KF+DD+WGPV A
Sbjct: 190 NNPAHERSKYEYDVDKEMTLVKFVDDQWGPV----------------------------A 249
Query: 261 RFMEDWFKQKGTGTLHPGESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATR 320
R MEDWF+++ ES PRRVS+I+ + Y Q+L+E+A+S S G+ TR
Sbjct: 250 RIMEDWFERENGCRSVDVES-----PRRVSSIISDPY--DQDLMEMASSLLSTGGKTVTR 309
Query: 321 VLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNE 380
+ S++ RVR+ R AD+P+FVSAFCQ+NCGDVSPN LGAFC+DTGLPC+FN STCGGKNE
Sbjct: 310 MSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNE 369
Query: 381 LCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNK 440
CYGRGPGYPDEFESTRIIGE+QF+KA DLF+KASE++ GKVD+RH+YVDFS LEV++N
Sbjct: 370 QCYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTING 429
Query: 441 QGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISC 500
Q G +EVVKTCPAAMGF FAAGTTDGPGAFDFKQGDD+GN FW+LVRN+LK P EQ+ C
Sbjct: 430 QNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRC 489
Query: 501 QSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILNVPGEFTTMAGRRLRDAVKTVLT 560
Q PKPILLDTGEMK PYDWAPSILP+QILRIGQLVIL VPGEFTTMAGRRLRDAVKTVL
Sbjct: 490 QRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLK 549
Query: 561 TGAK-KEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKL 620
G+ +EF+ VVIAGLTN+YSQY+ TFEEY++QRYEGASTLYGPHTL YIQEFKKL
Sbjct: 550 EGSNGREFS----VVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKL 609
Query: 621 AQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRG-NL 680
A L+ PGPQPPDLL +QISLL PV+ DMTP+G +FGDV DVP S F++G ++
Sbjct: 610 ANDLLSAQTTDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDVPRLSKFRKGADI 669
Query: 681 VKVTFWTGCPRNDLMTEGTFALVE--ILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYA 740
V+V F + PRNDLMTEGTFALVE + + TWVP YDDDDFCLRFKWSRP LS QS A
Sbjct: 670 VRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQSTA 729
Query: 741 TIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 779
TIEWRIP+TA GVYRI HFG+AK+ + SI HF+GSSSAFVV
Sbjct: 730 TIEWRIPETASPGVYRITHFGSAKTPISSIHHFSGSSSAFVV 732
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
F4HQM3 | 0.0e+00 | 75.27 | Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1 | [more] |
Q304B9 | 0.0e+00 | 73.35 | Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1 | [more] |
Q0JL46 | 0.0e+00 | 70.36 | Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1... | [more] |
F4KHQ8 | 6.6e-310 | 69.69 | Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1 | [more] |
Q55G11 | 7.1e-171 | 43.86 | Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0LY63 | 0.0e+00 | 99.61 | Neutral ceramidase OS=Cucumis sativus OX=3659 GN=Csa_1G527930 PE=3 SV=1 | [more] |
A0A1S3C5F7 | 0.0e+00 | 95.50 | Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103497106 PE=3 SV=1 | [more] |
A0A6J1HIT9 | 0.0e+00 | 89.62 | Neutral ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111464833 PE=3 SV=1 | [more] |
A0A6J1DAR9 | 0.0e+00 | 89.10 | Neutral ceramidase OS=Momordica charantia OX=3673 GN=LOC111018960 PE=3 SV=1 | [more] |
A0A6J1I331 | 0.0e+00 | 88.97 | Neutral ceramidase OS=Cucurbita maxima OX=3661 GN=LOC111469470 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
XP_004153679.2 | 0.0e+00 | 99.61 | neutral ceramidase 2 [Cucumis sativus] >KGN65777.1 hypothetical protein Csa_0233... | [more] |
XP_008457412.1 | 0.0e+00 | 95.50 | PREDICTED: neutral ceramidase-like isoform X1 [Cucumis melo] | [more] |
XP_038892835.1 | 0.0e+00 | 92.82 | neutral ceramidase 2-like [Benincasa hispida] | [more] |
KAG6583392.1 | 0.0e+00 | 89.87 | Neutral ceramidase 1, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
KAG7019159.1 | 0.0e+00 | 89.74 | Neutral ceramidase 1 [Cucurbita argyrosperma subsp. argyrosperma] | [more] |