CSPI01G13090 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G13090
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	RING-CH-type domain-containing protein
Location	Chr1: 8554307 .. 8555985 (-)
RNA-Seq Expression	CSPI01G13090
Synteny	CSPI01G13090

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTAATATGATAGTATCAATAAGAGTTAGAAAAGCTTACAAATCTGGTAGCTTCGATTGCTTTTAAGACAGTAACAATTGATATTTTCTGCTACTTCCCTTTGCTTTCTTCTCTGGGTTTACATGAAAGTGTCACTTTCGCTACAAGGAGGAGGGGCTTGTGATGGGGGTGTGCCACACTGGATAATACCCCTTCCTCTCGCAAATTCTTGTCTTGTCTATACAAACAACAAGTGGGAAAAGCTTATTTCAGACAGTAAGTTGAAATTCCGTGTAGTTGTCAGTCTCCCTTGCTTCATCCTAAAGAAAGGCAACAATCTGATTCTCTCCCTTTTAATCTTTTAAACTCTTCTATCATTTGTTCCATACTCGTTTCATCTAACGGATCTCAAGATTATTTGAAGCAGCAGTTCAATTACCTTAGGACTCCCAGCTTCCTTCTCACTATTCAATTACCTTGGCAAATTCCATGATATGTAAACTTGTTCGTGGAGACCAAACATCGATATGATTGAACAAAATGAAGGATCAAGTGGGATAACAAGCCCAGATGAACCTAAGACAATTGTTGCATCGCCGTCGAGACCTCGTGAAGCGAAAGGGGTAATTGGAGGGGAAGGTGGGACCTGTGTGCGTGAGAATGAGGAGAATAGCAAGAGGCATGGAGTGGAGATGCTAGAGACCAGCTCACAGCCAGAGAGTCATCAAAACTGCATCATTGATATAAAAACCAATGGAGAGTCAGTGGATGATGAAGATTCTAGTGAAGAAAAGGTTTGCAGGATATGTCATTTGGGCTCCGAAAACGAGTCGATAACTTCAGAACTCATACACCTCGGGTGTAGCTGCAAAGATGAGCTTGGAATATCACATCGTGACTGCGCCGAGATTTGGTTTATGCATCGAGGCAACAGGTAAAATTCCTATGACGATCTTCATCCTCTCCTCTCTTCACAGTTTCCAATAACAGATATGGTGCTCTTAATATAACTCCAATTCTGTATCATCCCTTTCTAATGGTAGACAATGTGAGATTTGTGGTCAGACTGCAACAAATGTGAGGAGAAACAGGAGTAGCCATTTTGCAATACATCGGAATGAGAGGAGATTGGTAGCAAGCTCAACAGTGTCGACAGTGTCCTTGGACAATGAGGTTTCATTATCATGTTGCTGGGGGCAGCGCTTCTGTAATTTCCTATTAACATGTACAGTTCTAGCCTTCCTCCTCCCATGGTTTTTTCGTGCCGATTTCTTTGGATGATGAGCGATGGTCTTTCTCAAACATCCTATCATCACATTAATCAGCTACAAAGATTCCAGACGTGCAATCCCGGACCTGGGAATAAGCAGATGCAACAGTTTCACACAAGGAAGAATTGACAAACATGTAAATATATAGAACAGTGCATATAAAAATGCTAACTCACAATAATTCTATATCAAGAGTAAGAAAATTAAAAATAGTTAGCCAGAGAGAGAGAGAAAGAGATTGCAATGCAAACAATAGAAATGGTTATAATTTACTAAACTATTTACCTTTCCATATTAAAATGTATTTTCTAAGTCATGTCTTTGTCGAGGCATCAAACAAAAAGGCAGGCACTAAGCATAGAGATTAAAAAAATAGATCTTTATATGGCGGCTCCTGCTTTTCTAGTTTCTAACCATAATCACCTAGCA

mRNA sequence

AGTAATATGATAGTATCAATAAGAGTTAGAAAAGCTTACAAATCTGGTAGCTTCGATTGCTTTTAAGACAGTAACAATTGATATTTTCTGCTACTTCCCTTTGCTTTCTTCTCTGGGTTTACATGAAAGTGTCACTTTCGCTACAAGGAGGAGGGGCTTGTGATGGGGGTGTGCCACACTGGATAATACCCCTTCCTCTCGCAAATTCTTGTCTTGTCTATACAAACAACAAGTGGGAAAAGCTTATTTCAGACAGTAAGTTGAAATTCCGTGTAGTTGTCAGTCTCCCTTGCTTCATCCTAAAGAAAGGCAACAATCTGATTCTCTCCCTTTTAATCTTTTAAACTCTTCTATCATTTGTTCCATACTCGTTTCATCTAACGGATCTCAAGATTATTTGAAGCAGCAGTTCAATTACCTTAGGACTCCCAGCTTCCTTCTCACTATTCAATTACCTTGGCAAATTCCATGATATGTAAACTTGTTCGTGGAGACCAAACATCGATATGATTGAACAAAATGAAGGATCAAGTGGGATAACAAGCCCAGATGAACCTAAGACAATTGTTGCATCGCCGTCGAGACCTCGTGAAGCGAAAGGGGTAATTGGAGGGGAAGGTGGGACCTGTGTGCGTGAGAATGAGGAGAATAGCAAGAGGCATGGAGTGGAGATGCTAGAGACCAGCTCACAGCCAGAGAGTCATCAAAACTGCATCATTGATATAAAAACCAATGGAGAGTCAGTGGATGATGAAGATTCTAGTGAAGAAAAGGTTTGCAGGATATGTCATTTGGGCTCCGAAAACGAGTCGATAACTTCAGAACTCATACACCTCGGGTGTAGCTGCAAAGATGAGCTTGGAATATCACATCGTGACTGCGCCGAGATTTGGTTTATGCATCGAGGCAACAGACAATGTGAGATTTGTGGTCAGACTGCAACAAATGTGAGGAGAAACAGGAGTAGCCATTTTGCAATACATCGGAATGAGAGGAGATTGGTAGCAAGCTCAACAGTGTCGACAGTGTCCTTGGACAATGAGGTTTCATTATCATGTTGCTGGGGGCAGCGCTTCTGTAATTTCCTATTAACATGTACAGTTCTAGCCTTCCTCCTCCCATGGTTTTTTCGTGCCGATTTCTTTGGATGATGAGCGATGGTCTTTCTCAAACATCCTATCATCACATTAATCAGCTACAAAGATTCCAGACGTGCAATCCCGGACCTGGGAATAAGCAGATGCAACAGTTTCACACAAGGAAGAATTGACAAACATGTAAATATATAGAACAGTGCATATAAAAATGCTAACTCACAATAATTCTATATCAAGAGTAAGAAAATTAAAAATAGTTAGCCAGAGAGAGAGAGAAAGAGATTGCAATGCAAACAATAGAAATGGTTATAATTTACTAAACTATTTACCTTTCCATATTAAAATGTATTTTCTAAGTCATGTCTTTGTCGAGGCATCAAACAAAAAGGCAGGCACTAAGCATAGAGATTAAAAAAATAGATCTTTATATGGCGGCTCCTGCTTTTCTAGTTTCTAACCATAATCACCTAGCA

Coding sequence (CDS)

ATGATTGAACAAAATGAAGGATCAAGTGGGATAACAAGCCCAGATGAACCTAAGACAATTGTTGCATCGCCGTCGAGACCTCGTGAAGCGAAAGGGGTAATTGGAGGGGAAGGTGGGACCTGTGTGCGTGAGAATGAGGAGAATAGCAAGAGGCATGGAGTGGAGATGCTAGAGACCAGCTCACAGCCAGAGAGTCATCAAAACTGCATCATTGATATAAAAACCAATGGAGAGTCAGTGGATGATGAAGATTCTAGTGAAGAAAAGGTTTGCAGGATATGTCATTTGGGCTCCGAAAACGAGTCGATAACTTCAGAACTCATACACCTCGGGTGTAGCTGCAAAGATGAGCTTGGAATATCACATCGTGACTGCGCCGAGATTTGGTTTATGCATCGAGGCAACAGACAATGTGAGATTTGTGGTCAGACTGCAACAAATGTGAGGAGAAACAGGAGTAGCCATTTTGCAATACATCGGAATGAGAGGAGATTGGTAGCAAGCTCAACAGTGTCGACAGTGTCCTTGGACAATGAGGTTTCATTATCATGTTGCTGGGGGCAGCGCTTCTGTAATTTCCTATTAACATGTACAGTTCTAGCCTTCCTCCTCCCATGGTTTTTTCGTGCCGATTTCTTTGGATGA

Protein sequence

MIEQNEGSSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEENSKRHGVEMLETSSQPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGISHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNEVSLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFFG*

Homology

[Close]

BLAST of CSPI01G13090 vs. ExPASy Swiss-Prot
Match: F4JKK0 (Probable E3 ubiquitin ligase SUD1 OS=Arabidopsis thaliana OX=3702 GN=SUD1 PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.5e-06
Identity = 33/115 (28.70%), Postives = 46/115 (40.00%), Query Frame = 0


Query: 27  PREAKGVIGGEGGTCVRENEENSKRHGVEMLETSSQPESHQNCIIDIKTNGESVDDEDSS 86
           P ++  + G      V E   +S        + S  P S+ +  +   T    VDD D  
Sbjct: 5   PADSLSISGAAASEVVSEPSVSSSSSSSSPNQASPNPFSNMDPAVSTATGSRYVDD-DED 64

Query: 87  EEKVCRICHLGSENESITSELIHLGCSCKDELGISHRDCAEIWFMHRGNRQCEIC 142
           EE VCRIC     N       +   C+C   +   H+DC   W  H   RQCE+C
Sbjct: 65  EEDVCRIC----RNPGDADNPLRYPCACSGSIKFVHQDCLLQWLNHSNARQCEVC 114

[Close]

BLAST of CSPI01G13090 vs. ExPASy Swiss-Prot
Match: Q32L65 (E3 ubiquitin-protein ligase MARCHF2 OS=Bos taurus OX=9913 GN=MARCHF2 PE=2 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 4.9e-05
Identity = 25/71 (35.21%), Postives = 32/71 (45.07%), Query Frame = 0


Query: 83  EDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGISHRDCAEIWFMHRGNRQCEICG 142
           E  S+   CRICH G+  ES+ S      C C   LG  H+ C E W        CE+C 
Sbjct: 56  ETPSDGPFCRICHEGANGESLLSP-----CGCSGTLGAVHKSCLERWLSSSNTSYCELCH 115

Query: 143 QTATNVRRNRS 154
                 +R+RS
Sbjct: 116 TEFAVEKRSRS 121

[Close]

BLAST of CSPI01G13090 vs. ExPASy Swiss-Prot
Match: Q68FA7 (E3 ubiquitin-protein ligase MARCHF3 OS=Xenopus tropicalis OX=8364 GN=marchf3 PE=2 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 3.2e-04
Identity = 22/69 (31.88%), Postives = 31/69 (44.93%), Query Frame = 0


Query: 73  IKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGISHRDCAEIWFMH 132
           + T   ++  +  ++  +CRICH GS  E + S      C C   LG  HR C E W   
Sbjct: 51  LSTVVRTLTTQSFNDRPMCRICHEGSTQEDLLSP-----CECTGTLGTIHRSCLEHWLSS 110

Query: 133 RGNRQCEIC 142
                CE+C
Sbjct: 111 SNTSYCELC 114

[Close]

BLAST of CSPI01G13090 vs. ExPASy TrEMBL
Match: A0A0A0LUV6 (RING-CH-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G096610 PE=4 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 8.9e-119
Identity = 213/214 (99.53%), Postives = 213/214 (99.53%), Query Frame = 0


Query: 1   MIEQNEGSSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEENSKRHGVEMLETS 60
           MIEQNEGSSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEEN KRHGVEMLETS
Sbjct: 1   MIEQNEGSSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEENRKRHGVEMLETS 60

Query: 61  SQPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGI 120
           SQPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGI
Sbjct: 61  SQPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGI 120

Query: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNEV 180
           SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNEV
Sbjct: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNEV 180

Query: 181 SLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFFG 215
           SLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFFG
Sbjct: 181 SLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFFG 214

[Close]

BLAST of CSPI01G13090 vs. ExPASy TrEMBL
Match: A0A1S3CJ88 (probable E3 ubiquitin ligase SUD1 OS=Cucumis melo OX=3656 GN=LOC103501475 PE=4 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 3.1e-111
Identity = 203/214 (94.86%), Postives = 209/214 (97.66%), Query Frame = 0


Query: 1   MIEQNEGSSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEENSKRHGVEMLETS 60
           MIEQNEGSSGITSP+EP+TI ASPSRPREAKGVIGGEGGT VRENEENSKRHG EMLE S
Sbjct: 1   MIEQNEGSSGITSPNEPETIAASPSRPREAKGVIGGEGGTYVRENEENSKRHGEEMLE-S 60

Query: 61  SQPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGI 120
           SQPES QNCIIDIK+NGESVDDEDSSEEKVCRICHLGSE+ESITS+LIHLGCSCKDELGI
Sbjct: 61  SQPESRQNCIIDIKSNGESVDDEDSSEEKVCRICHLGSEHESITSDLIHLGCSCKDELGI 120

Query: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNEV 180
           SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASST+STVSLDNEV
Sbjct: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTMSTVSLDNEV 180

Query: 181 SLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFFG 215
           SLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFFG
Sbjct: 181 SLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFFG 213

[Close]

BLAST of CSPI01G13090 vs. ExPASy TrEMBL
Match: A0A6J1D2I2 (uncharacterized protein LOC111016745 OS=Momordica charantia OX=3673 GN=LOC111016745 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.4e-79
Identity = 159/216 (73.61%), Postives = 174/216 (80.56%), Query Frame = 0


Query: 1   MIEQNEGSSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEEN--SKRHGVEMLE 60
           MI+Q+EGSSG  + DE  T++A P RP+EAK V GG     V+ + EN   KRHG E   
Sbjct: 1   MIKQDEGSSGTPNRDESVTVLALPPRPQEAKEVTGG----VVQNHNENEAGKRHGEE--- 60

Query: 61  TSSQPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDEL 120
                ESH NCIIDIK++GESVD EDSSEEKVCRICHL SE++SI S+LI LGC CKDEL
Sbjct: 61  ---SKESHHNCIIDIKSSGESVDGEDSSEEKVCRICHLSSEHQSIASDLIQLGCRCKDEL 120

Query: 121 GISHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDN 180
           GISHRDCAEIWFMHRGNRQCEICGQTATNVR  RS  F I RNERRLVASSTVSTVSLD+
Sbjct: 121 GISHRDCAEIWFMHRGNRQCEICGQTATNVRGIRSGGFMIQRNERRLVASSTVSTVSLDS 180

Query: 181 EVSLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFFG 215
           + SLSCCWGQRFCNFLLTCTVLAFLLPWFFR DFFG
Sbjct: 181 DDSLSCCWGQRFCNFLLTCTVLAFLLPWFFRLDFFG 206

[Close]

BLAST of CSPI01G13090 vs. ExPASy TrEMBL
Match: A0A6J1ESF7 (uncharacterized protein LOC111437147 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437147 PE=4 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 4.2e-76
Identity = 149/213 (69.95%), Postives = 171/213 (80.28%), Query Frame = 0


Query: 1   MIEQNEGSSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEENSKRHGVEMLETS 60
           MIEQ EGSS  TSPDEP+ ++ SP+RP EAK V+         E+E   +  G    ++S
Sbjct: 1   MIEQEEGSSRTTSPDEPEIVIVSPTRPEEAKEVLARS------EHEHEHEHEGAG--KSS 60

Query: 61  SQPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGI 120
            QPESH  CIIDIK+ GES+DD +SS EKVCRICHL S+ ES++S+L++LGC CK ELG+
Sbjct: 61  RQPESHHTCIIDIKSTGESMDDANSSNEKVCRICHLSSD-ESVSSDLVNLGCRCKGELGM 120

Query: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNEV 180
           SHRDCAEIWFMHRGNRQCEICGQTATNVR NRSS F I RNERR+VASS+VS VSLDNEV
Sbjct: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRGNRSSVFMIQRNERRMVASSSVSAVSLDNEV 180

Query: 181 SLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFF 214
            LSCCWGQRFCNFLLTCTVLAFLLPWFFR +FF
Sbjct: 181 PLSCCWGQRFCNFLLTCTVLAFLLPWFFRFNFF 204

[Close]

BLAST of CSPI01G13090 vs. ExPASy TrEMBL
Match: A0A6J1JNU4 (uncharacterized protein LOC111486158 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486158 PE=4 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 1.0e-74
Identity = 147/213 (69.01%), Postives = 169/213 (79.34%), Query Frame = 0


Query: 1   MIEQNEGSSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEENSKRHGVEMLETS 60
           MIEQ EGSS  TSPDEP+ ++  P+RP EAK V+     +   E+E   K        +S
Sbjct: 1   MIEQEEGSSRTTSPDEPEIVIVLPTRPEEAKEVLARSERSHEHEHEGAGK--------SS 60

Query: 61  SQPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGI 120
            QPES   CIIDIK+ GES+DD +SS+EKVCRICHL S+ +S+TS+L++LGC CK ELG+
Sbjct: 61  RQPESQHTCIIDIKSTGESMDDANSSDEKVCRICHLSSD-QSVTSDLVNLGCRCKGELGM 120

Query: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNEV 180
           SHRDCAEIWF HRGNRQCEICGQTATNVR NRSS F I RNERR+VASS+VS VSLDNEV
Sbjct: 121 SHRDCAEIWFTHRGNRQCEICGQTATNVRGNRSSVFMIQRNERRMVASSSVSAVSLDNEV 180

Query: 181 SLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFF 214
           SLSCCWGQRFCNFLLTCTVLAFLLPWFFR +FF
Sbjct: 181 SLSCCWGQRFCNFLLTCTVLAFLLPWFFRFNFF 204

[Close]

BLAST of CSPI01G13090 vs. NCBI nr
Match: XP_011653704.1 (probable E3 ubiquitin ligase SUD1 isoform X1 [Cucumis sativus] >KGN64779.1 hypothetical protein Csa_013679 [Cucumis sativus])

HSP 1 Score: 436.0 bits (1120), Expect = 1.8e-118
Identity = 213/214 (99.53%), Postives = 213/214 (99.53%), Query Frame = 0


Query: 1   MIEQNEGSSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEENSKRHGVEMLETS 60
           MIEQNEGSSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEEN KRHGVEMLETS
Sbjct: 1   MIEQNEGSSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEENRKRHGVEMLETS 60

Query: 61  SQPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGI 120
           SQPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGI
Sbjct: 61  SQPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGI 120

Query: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNEV 180
           SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNEV
Sbjct: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNEV 180

Query: 181 SLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFFG 215
           SLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFFG
Sbjct: 181 SLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFFG 214

[Close]

BLAST of CSPI01G13090 vs. NCBI nr
Match: XP_008463280.1 (PREDICTED: probable E3 ubiquitin ligase SUD1 [Cucumis melo])

HSP 1 Score: 411.0 bits (1055), Expect = 6.3e-111
Identity = 203/214 (94.86%), Postives = 209/214 (97.66%), Query Frame = 0


Query: 1   MIEQNEGSSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEENSKRHGVEMLETS 60
           MIEQNEGSSGITSP+EP+TI ASPSRPREAKGVIGGEGGT VRENEENSKRHG EMLE S
Sbjct: 1   MIEQNEGSSGITSPNEPETIAASPSRPREAKGVIGGEGGTYVRENEENSKRHGEEMLE-S 60

Query: 61  SQPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGI 120
           SQPES QNCIIDIK+NGESVDDEDSSEEKVCRICHLGSE+ESITS+LIHLGCSCKDELGI
Sbjct: 61  SQPESRQNCIIDIKSNGESVDDEDSSEEKVCRICHLGSEHESITSDLIHLGCSCKDELGI 120

Query: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNEV 180
           SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASST+STVSLDNEV
Sbjct: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTMSTVSLDNEV 180

Query: 181 SLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFFG 215
           SLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFFG
Sbjct: 181 SLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFFG 213

[Close]

BLAST of CSPI01G13090 vs. NCBI nr
Match: XP_038887762.1 (probable E3 ubiquitin ligase SUD1 [Benincasa hispida])

HSP 1 Score: 371.7 bits (953), Expect = 4.3e-99
Identity = 184/214 (85.98%), Postives = 195/214 (91.12%), Query Frame = 0


Query: 1   MIEQNEGSSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEENSKRHGVEMLETS 60
           MI+Q+EGSSGITSP+E  TI ASPSRP EAKGVIGGE    V ENEE+ KRHG EM E+S
Sbjct: 1   MIKQDEGSSGITSPNESGTIAASPSRPHEAKGVIGGESENRVHENEEDGKRHGEEMQESS 60

Query: 61  SQPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGI 120
            QPES+QNCIIDIK+NG S+D EDSSEEKVCRICHL SE+ESITS+LIHLGCSCKDELGI
Sbjct: 61  RQPESNQNCIIDIKSNGGSMDGEDSSEEKVCRICHLSSEHESITSDLIHLGCSCKDELGI 120

Query: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNEV 180
           SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSS FAI +NERRLVASSTVSTVSLDNEV
Sbjct: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSGFAIQQNERRLVASSTVSTVSLDNEV 180

Query: 181 SLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFFG 215
           SLSCCWGQRFCNFLLTCTVLAFLLPWFFR DFFG
Sbjct: 181 SLSCCWGQRFCNFLLTCTVLAFLLPWFFRVDFFG 214

[Close]

BLAST of CSPI01G13090 vs. NCBI nr
Match: XP_022147939.1 (uncharacterized protein LOC111016745 [Momordica charantia])

HSP 1 Score: 305.8 bits (782), Expect = 2.9e-79
Identity = 159/216 (73.61%), Postives = 174/216 (80.56%), Query Frame = 0


Query: 1   MIEQNEGSSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEEN--SKRHGVEMLE 60
           MI+Q+EGSSG  + DE  T++A P RP+EAK V GG     V+ + EN   KRHG E   
Sbjct: 1   MIKQDEGSSGTPNRDESVTVLALPPRPQEAKEVTGG----VVQNHNENEAGKRHGEE--- 60

Query: 61  TSSQPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDEL 120
                ESH NCIIDIK++GESVD EDSSEEKVCRICHL SE++SI S+LI LGC CKDEL
Sbjct: 61  ---SKESHHNCIIDIKSSGESVDGEDSSEEKVCRICHLSSEHQSIASDLIQLGCRCKDEL 120

Query: 121 GISHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDN 180
           GISHRDCAEIWFMHRGNRQCEICGQTATNVR  RS  F I RNERRLVASSTVSTVSLD+
Sbjct: 121 GISHRDCAEIWFMHRGNRQCEICGQTATNVRGIRSGGFMIQRNERRLVASSTVSTVSLDS 180

Query: 181 EVSLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFFG 215
           + SLSCCWGQRFCNFLLTCTVLAFLLPWFFR DFFG
Sbjct: 181 DDSLSCCWGQRFCNFLLTCTVLAFLLPWFFRLDFFG 206

[Close]

BLAST of CSPI01G13090 vs. NCBI nr
Match: KAG7022941.1 (hypothetical protein SDJN02_16677 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 294.7 bits (753), Expect = 6.6e-76
Identity = 150/213 (70.42%), Postives = 169/213 (79.34%), Query Frame = 0


Query: 1   MIEQNEGSSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEENSKRHGVEMLETS 60
           MIEQ EGSS  TSPDEP+ ++ SP RP EAK V+     +   E+E   K        +S
Sbjct: 1   MIEQEEGSSRTTSPDEPEIVIVSPMRPEEAKEVLARSERSDEHEHEGAGK--------SS 60

Query: 61  SQPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGI 120
            QPESH  CIIDIK+ GES+DD +SS EKVCRICHL S+ ES++SEL++LGC CK ELG+
Sbjct: 61  RQPESHHTCIIDIKSTGESMDDANSSNEKVCRICHLSSD-ESVSSELVNLGCRCKGELGM 120

Query: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNEV 180
           SHRDCAEIWFMHRGNRQCEICGQTATNVR NRSS F I RNERR+VASS+VS VSLDNEV
Sbjct: 121 SHRDCAEIWFMHRGNRQCEICGQTATNVRGNRSSVFMIQRNERRMVASSSVSAVSLDNEV 180

Query: 181 SLSCCWGQRFCNFLLTCTVLAFLLPWFFRADFF 214
            LSCCWGQRFCNFLLTCTVLAFLLPWFFR +FF
Sbjct: 181 PLSCCWGQRFCNFLLTCTVLAFLLPWFFRFNFF 204

[Close]

BLAST of CSPI01G13090 vs. TAIR 10
Match: AT5G01070.2 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 4.5e-22
Identity = 59/143 (41.26%), Postives = 79/143 (55.24%), Query Frame = 0


Query: 71  IDIKTNGESVDDED--SSEEKVCRICHLGSE-NESITSELIHLGCSCKDELGISHRDCAE 130
           +D +T+ ESV       S EK CRICHLG E +     + + LGCSCKD+LG  H+ CA+
Sbjct: 56  LDDETDTESVASGSIRGSPEKDCRICHLGLESSRHECGDPMVLGCSCKDDLGYVHKQCAD 115

Query: 131 IWFMHRGNRQCEICGQTATNVRR--NRSSHFAIHRNERRLVASSTVSTVSLDNEVSLSCC 190
            WF  +GN+ CEIC   A N  +  N      +  N   + A +T + V+  +      C
Sbjct: 116 TWFKIKGNKTCEICRSIAQNFFKVDNEIGQTTVETNVDDVEAGNTPTMVATSDSDDRRLC 175

Query: 191 WGQRFCNFLLTCTVLAFLLPWFF 209
            G RF NFLLTC V AF++ WFF
Sbjct: 176 RGNRFLNFLLTCMVCAFVISWFF 198

[Close]

BLAST of CSPI01G13090 vs. TAIR 10
Match: AT5G05830.1 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 93.2 bits (230), Expect = 2.7e-19
Identity = 51/125 (40.80%), Postives = 68/125 (54.40%), Query Frame = 0


Query: 85  SSEEKVCRICHLGSENESITSEL-IHLGCSCKDELGISHRDCAEIWFMHRGNRQCEICGQ 144
           S  E+ CRICH+  +  ++ S + I LGCSCK +L  +H+ CAE WF  +GN+ CE+CG 
Sbjct: 73  SQSERDCRICHMSLDAANLESGVPIELGCSCKADLAAAHKHCAETWFKIKGNKICEVCGS 132

Query: 145 TATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNEVSLSCCWGQRFCNFLLTCTVLAFL 204
            A NV  +        RNE   V + T+ T         S   G RF NFLL C V AF+
Sbjct: 133 IAGNVVGSVEVESEESRNEANGVENLTLRTSGPRLVEGRSFWQGHRFLNFLLACMVFAFV 192

Query: 205 LPWFF 209
           + W F
Sbjct: 193 ISWLF 197

[Close]

BLAST of CSPI01G13090 vs. TAIR 10
Match: AT2G37950.1 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 84.3 bits (207), Expect = 1.3e-16
Identity = 50/136 (36.76%), Postives = 66/136 (48.53%), Query Frame = 0


Query: 76  NGESVDDEDSS--EEKVCRICHLGSENESITSELIHLGCSCKDELGISHRDCAEIWFMHR 135
           +   +DD+D++   EK CRICHLG E        I LGCSCKD+L ++HR CAE WF  +
Sbjct: 67  SSSEIDDDDATAPPEKDCRICHLGVETSG--GGAIELGCSCKDDLAVAHRQCAETWFKIK 126

Query: 136 GNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNEVSLSCCW-GQRFCN 195
           G++ CEIC   A NV    +        ER L      +         +   W  QR  N
Sbjct: 127 GDKTCEICQSVARNV-GGANEMVGSTMEERELRNGEETAAGEGGGATVVENRWQPQRVVN 186

Query: 196 FLLTCTVLAFLLPWFF 209
            +L C V  F + W F
Sbjct: 187 LVLACMVFGFFISWIF 199

[Close]

BLAST of CSPI01G13090 vs. TAIR 10
Match: AT2G34200.1 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 3.7e-16
Identity = 53/134 (39.55%), Postives = 69/134 (51.49%), Query Frame = 0


Query: 88  EKVCRICHLGS--------ENESITSELIHLGCSCKDELGISHRDCAEIWFMHRGNRQCE 147
           E++CRICH GS        +++S++ ELI +GC CK+ELG++H  CAE WF  RGN  CE
Sbjct: 99  ERICRICHFGSDQSPEASGDDKSVSPELIEIGCKCKNELGLAHFHCAEAWFKLRGNSVCE 158

Query: 148 ICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNE----VSLSCCWGQRFCNFLL 207
           ICG TA NV       ++  R+             +LD         SCC    F  FLL
Sbjct: 159 ICGCTAKNVTVRLMEDWSGERDN------------TLDGRRRRGRGQSCC---IFMVFLL 213

Query: 208 TCTVLAFLLPWFFR 210
           T      LL WFF+
Sbjct: 219 T----ILLLHWFFK 213

[Close]

BLAST of CSPI01G13090 vs. TAIR 10
Match: AT5G59000.1 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 6.5e-13
Identity = 59/207 (28.50%), Postives = 85/207 (41.06%), Query Frame = 0


Query: 8   SSGITSPDEPKTIVASPSRPREAKGVIGGEGGTCVRENEENSKRHGVEM------LETSS 67
           S G+ +P++         R R  K           R  ++  K+ G +       LET+ 
Sbjct: 44  SGGVPAPEKQLHSGGKKRRTRRRK-----------RRKKKKKKKGGRDCRICHLPLETNK 103

Query: 68  QPESHQNCIIDIKTNGESVDDEDSSEEKVCRICHLGSENESITSELIHLGCSCKDELGIS 127
           + E       D   + E  +DE+  EE+         E E      + LGCSCK +LG++
Sbjct: 104 EAEDEDEEEEDDSDDDEDEEDEEEEEEE---------EEEEYYGLPLQLGCSCKGDLGVA 163

Query: 128 HRDCAEIWFMHRGNRQCEICGQTATNVRRNRSSHFAIHRNERRLVASSTVSTVSLDNEVS 187
           H  CAE WF  +GN  CEICG  A NV   +S+  +      +  A       SL     
Sbjct: 164 HSKCAETWFKIKGNMTCEICGAMALNVAGEQSNPESTASTHSQAAAGQ-----SLTQTEP 223

Query: 188 LSCCWGQRFCNFLLTCTVLAFLLPWFF 209
                G+   NFLL   V AF++ W F
Sbjct: 224 RGIWHGRPVMNFLLAAMVFAFVVSWLF 225

The following BLAST results are available for this feature:

BLAST of CSPI01G13090 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JKK0	1.5e-06	28.70	Probable E3 ubiquitin ligase SUD1 OS=Arabidopsis thaliana OX=3702 GN=SUD1 PE=1 S...	[more]
Q32L65	4.9e-05	35.21	E3 ubiquitin-protein ligase MARCHF2 OS=Bos taurus OX=9913 GN=MARCHF2 PE=2 SV=1	[more]
Q68FA7	3.2e-04	31.88	E3 ubiquitin-protein ligase MARCHF3 OS=Xenopus tropicalis OX=8364 GN=marchf3 PE=...	[more]

BLAST of CSPI01G13090 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LUV6	8.9e-119	99.53	RING-CH-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G09661...	[more]
A0A1S3CJ88	3.1e-111	94.86	probable E3 ubiquitin ligase SUD1 OS=Cucumis melo OX=3656 GN=LOC103501475 PE=4 S...	[more]
A0A6J1D2I2	1.4e-79	73.61	uncharacterized protein LOC111016745 OS=Momordica charantia OX=3673 GN=LOC111016...	[more]
A0A6J1ESF7	4.2e-76	69.95	uncharacterized protein LOC111437147 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1JNU4	1.0e-74	69.01	uncharacterized protein LOC111486158 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of CSPI01G13090 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011653704.1	1.8e-118	99.53	probable E3 ubiquitin ligase SUD1 isoform X1 [Cucumis sativus] >KGN64779.1 hypot...	[more]
XP_008463280.1	6.3e-111	94.86	PREDICTED: probable E3 ubiquitin ligase SUD1 [Cucumis melo]	[more]
XP_038887762.1	4.3e-99	85.98	probable E3 ubiquitin ligase SUD1 [Benincasa hispida]	[more]
XP_022147939.1	2.9e-79	73.61	uncharacterized protein LOC111016745 [Momordica charantia]	[more]
KAG7022941.1	6.6e-76	70.42	hypothetical protein SDJN02_16677 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CSPI01G13090 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01070.2	4.5e-22	41.26	RING/FYVE/PHD zinc finger superfamily protein	[more]
AT5G05830.1	2.7e-19	40.80	RING/FYVE/PHD zinc finger superfamily protein	[more]
AT2G37950.1	1.3e-16	36.76	RING/FYVE/PHD zinc finger superfamily protein	[more]
AT2G34200.1	3.7e-16	39.55	RING/FYVE/PHD zinc finger superfamily protein	[more]
AT5G59000.1	6.5e-13	28.50	RING/FYVE/PHD zinc finger superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 90..142 e-value: 9.9E-12 score: 55.0
IPR011016	Zinc finger, RING-CH-type	PFAM	PF12906	RINGv	coord: 91..141 e-value: 1.5E-8 score: 34.6
IPR011016	Zinc finger, RING-CH-type	PROSITE	PS51292	ZF_RING_CH	coord: 83..148 score: 19.14863
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 74..154 e-value: 4.4E-16 score: 60.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..19
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..50
None	No IPR available	PANTHER	PTHR46214:SF8	OS01G0731800 PROTEIN	coord: 14..212
None	No IPR available	PANTHER	PTHR46214	ZINC FINGER, RING-CH-TYPE	coord: 14..212
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 85..145

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G13090.1	CSPI01G13090.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008270	zinc ion binding