CSPI01G04860 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G04860
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	eukaryotic translation initiation factor 2 subunit beta
Location	Chr1: 3111935 .. 3116391 (+)
RNA-Seq Expression	CSPI01G04860
Synteny	CSPI01G04860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGACGAAGTTCAGAATGATGCGAACGATCAGGTGGCAGATGTGAGTTTTTCTGTTCTTAACTATTATCTCTAAGAACATACATTTTCATTAAAAAATTTCACATATTGTGGGTATTCCCATTTTTGTGTGGGTATTCCCATTTTTGTTTTTCCCCTTTCAATTATTCATCCAAAGAAACCTAAATCTTTGAGTTGCCATTTGGCGAACCACACACTTGTGTGTTTCAACAGTCTAGGAGAATTTCATTCTATTATGTACAACTGTAGGTGTATAGCATTCTGGTAATCCAAGGAATAAATTGTATAACCCTTGAGGTGGCAGTCATTGATGATTTACCGAGTTACCCTAAATGCTATACTATTAGTTGTCTGGTCTTGTTTAAGATTACACAGTCCAATCTAGTTCTTGAAGTATTTTCTATTGGATTGTTCACTGGTGCTAAGGATCGAAGCTGCATTCTGCTGTCGAGTTGCAGGCTCATATCTTACTATGTCTCTTTATCAAGCAGTTACGAATTGTTGTTCATGTGCAAGCATAGATGCGACAGCATGATGCATTTATATGGTTTATTCTTGCAGATTGCCTTCGATCCAACTAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGTTGTACTTCAGGATCGGACTGATGAGATTGTTGACAAGTTAGCAGAGAAAACTGAAAGTCTGTCAGGTATGAAAAGTTGGCATTACATTCCATTTCTATTTCATGATATTTTTATTTTGTTGGTGAAATGGGAATTGTTGATGATTTTTTTTGTGTCCTGCAGTTTCTGATGGTTTGGAGACTACCACATTTTCTGGACTTAAAAAGAAGAAGAAAAAACAAGTATGTCTTACTTTTCTTTGGTCAAGTTCTATTTAGTGATTGATCTCATTTATTTTAATTATAGGTAGAGGTTAGCAACGCATATGGCATATGCAAACATAACTTAATCCAAGTATTATATACCAATGTGTTTAAAATTACATTTATATGAATGTTAAAACACAATCATAGTCCATGAATTTCAAACTTTTGTATCAAAAATCTTTTAATCTTTCAATTTTGTGTCCGATGGGTCATATATGACTAATTGATTTAGGAGCTATCAATTTATTTTATGTCCAGTTGAACACTAAACATCCAGTTTTGTAACTAGTAGATACAATAATTAAGCATCTAATACAAAACCTATTTGACATTCAGATTATATGTCTAATATGATGTTTATTTTATAATCATTTTGAATATGCAACAAACCTTTCAATACTTTAGCCAACTATTAGTCCCCAAATTGAGAATTTAGTGTTCTATTAGACGTTTCGCTATAAAATACAAATCTGAAAGTTTATTTGTTAAACTTATTTAACCTTATATGAATTTGTGTCTCCTGTGACATAAAGTTCTCAATTCTCCATGGAGAAAAATGTGCACTTTTAATGTTCATTTTTGTGGCCAATGATGGCTTTATTTGAGTATTAGTCTCATTTCATTATTTTAGTGAAAAGTTATGTTTCTGTTTCAAAAATCTTCATATGAATTTGTATCTCCTCTAATGCAAAGTTCTCGACCTGCACATGGAGAACCGAGCGTATTTTTAACGTTCAGTGACCATATAGACACAAATTTAAAAGTTTATCGGCTAAACTTATGTACCTTTATACTATGATATCCCCGTTGGCATGAACTTAAAAGTTTATTGGCTAAACTTGTTTAACCTTATATTTATGATATCCTCGTTGGCATGAACTTAAAAGTTTATTGGCTAAACTTGTTTAACCTTATATTTATTGTATCTTCTCTGACATAAAGTCCTTGATTTTTCATGGAGGAGATTGAAGAAAGATGAACATTGTTAGAATATGTCGTATCTTACTAAAAACTTAAAAAGAAAAAGAGAACAAAAGTTTCACATGGCTCACCATGCACCTCAAGCTCGTTTCATTAAAAAGGACGACTTGCTTTAGAAGCACGAGTACTTTTTGAAGAACTGCACAATTGGCCTTCTATTGTATGGAGTTTCTCATTGAAAACGAGTTGTGTATATAATTTATACTTTTTTGTTTGCCATCTCCTTTTCAACTGTAGCCCTTTTCGACTGAAAAGTGATTATTATGCCAATAAGACCAAGTGGGGGAAATGAAGACCATAAGGGAGTTATTCTACAGTTTCGTTATCCTTGGGAGGGAAGTGATTGTGTCACACCTTGCCCAGCACACGTCTTTGCTTGGTATGCGAGACACGGTATGAATTTGAGTATTCTGACGCGTTAAACACCAAAATACTCAGCTTGAGGTCCTTAAGCTTTGCTATAGCATTGAGAAAGTTGGTGCATAGTTGTGCAAGCTCTTGTCAATCTTTATACATGGGGGATGGGGATCATCAATTTTTAATATAGAACCTGTTTGAATCCGTTGATGCTCTTTTAGGGCTTAATGGTTGGAGAGTCGAAAAATGGAATTCTTGGAGGGTTTGAAAGATCTCAAGTGAAGGTTTGGTCTTGTTTTAGATTCTGTGTTTTTGTGGACTATGCGACTTTGCCTATTTGTAATTATTAGTTAGGACTTGTTTAGTTTGTATTGAGATGTTAGAATCTCATATTGAAAAAGTCAAGGGAACTCACACTTCTTATAAGATAAGATAAAGATAGATGAGTTACTCCTCCCATTGCCATTGAGGTGGAACCTCATACTATCAAAAAGTTTGATCTGAAGCTGTTTTTATAGTTTGCAATCTCTCCTTTTCATTGGTTTTTTTATAAATGAATTCTCGAAATTTATTTTTTTAGAAAAATCTTAATTACTGTTGACGAGTGTCCACTGGAATATATCTTGTTACCAACTATGAGTGATTGTGTTCACTTCTAATTTCGACCTTCGTTATAGTTTTTTTCCCTTCCCTCTTATTTATTTCAGATGAATATGTGATTTGCATTGGGTGCAAGAGTCCGGACACTATTTTTTCAAAGGAGAACCGGCTTTTCTTTCTCAGATGCGAGAAGGTAAGGTTTTTTTCTTTTGGTTTTTGTTTTGTATTTTTTCCCTTGCAGTGTGTTTATCCTATGGTCGTGATTTTTGTGTGAATTTGTGTTAGTATATTAAATTTACCATTACCCACTACAAGCCTTTTGGGTCAATCGATGATTTAAGAAGGTGTGTTACTTTAGATGTCCTGTATTCGAACCCTTGCAATGTTGTTTTCTCCCCAATTAAAATTAATTTCCACTTATTAGGCCTTTCAAGTATTTCAACCCCACAAGTAATGAGAGTATTGTGATATAGATTAAATTTACCTTCACTCATTAGCTCGAGTTTTGGATTGAATTGGCGATTTAAGATGCTATGAGAGTAGGTTACTTCAGGATGTCGTTGATTAAGAGAGTAGGGGTATAATGGTCCTACCATTTTATAAATGAAATCTCAGTATTTATTTTAAAACATCTTAATTACTGTTGACGAGTGTCCACTGGAATACATCTTGTTACCAACTATGAGTGATTTTGTTCACTTCTAATTTCGACCTTCGTTATAGTTTTTTCCCTTCCCTCTCATTTATTTAGTTATTTCAGATGAATATGTGATTTGCATTGGGTGCAAGAGTCTGGACACTCTTCTTTCAAAGGAGAACCGGCTTTTCTTTCTCAGGTGCGAGATGGTAAGGTTTTTTTTTTTTTTTTTTGGTTTTGGTTTTGTTTTTTTTCCCTTGCAGTCTATTTATTATATGGTAGTGATTTTTGTGTGAGTTTCTGTTAGATAGTATATTAAATTTACCCTTATCCACTAGGTTAAGTTTTTAGGTCAATCGGTGATTTAAGATCTTGTGTTGCTTTAGATGTTCTGTATTTAAACTCTTGCAATATTGTCTCCTCTCTAATTAAAATTCATTTCAATTTTCACTTGTTAGGTTTTTCAAGTATTTCAAGTTCACAAGTGACATGACTATTGTGATGTATGATTAAATTTACGTTCACTCATCCACACAAGTTTTGGATTGGATTAAGGATTGGATTAAGATGCTATCAGAGGTTACTTCAAGATGTCGTTGATTAAGAAAGTAGGGGTATAATGGTCCTGCCATTTTATAAATTTCAGTGATTATTTTGATGCATTGTTGGGGAAATGATGAATCGGAATATTTCGTCCATTGTAAATTTATTTATTATGCCTGATTGTTTATTTAAGATCTGATATTTGAATAGATAATTTTTTATTTATCCCGTGCTTCTTCCTAGTAGAATGACAGCCGAAGATCGGACCTTTTCACGTGCTTGAAAATCTATTATTTAATTGTTAGTTCTTTTTAATATACATGAATATGATTAACTAGAAGCTATCGGTATTCTTTTCAATTTCACAAGCTTCGCGTTCTTTACCCTGTTTTTATCATACGGCTTAAGGTGTTTTGTGTTCGATCAACAGTGGTTCTGGACGATCAGTTGCTCCAAT

mRNA sequence

ATGGAGGACGAAGTTCAGAATGATGCGAACGATCAGGTGGCAGATATTGCCTTCGATCCAACTAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGTTGTACTTCAGGATCGGACTGATGAGATTGTTGACAAGTTAGCAGAGAAAACTGAAAGTCTGTCAGTTTCTGATGGTTTGGAGACTACCACATTTTCTGGACTTAAAAAGAAGAAGAAAAAACAAACCATAAGGGAGTTATTCTACAGTTTCGTTATCCTTGGGAGGGAAGTGATTGTGTCACACCTTGCCCAGCACACGTCTTTGCTTGGTATGCGAGACACGGGCTTAATGGTTGGAGAGTCGAAAAATGGAATTCTTGGAGGGTTTGAAAGATCTCAAGTGAAGGTTTGGTCTTATGAATATGTGATTTGCATTGGGTGCAAGAGTCTGGACACTCTTCTTTCAAAGGAGAACCGGCTTTTCTTTCTCAGGTGCGAGATGTGGTTCTGGACGATCAGTTGCTCCAAT

Coding sequence (CDS)

ATGGAGGACGAAGTTCAGAATGATGCGAACGATCAGGTGGCAGATATTGCCTTCGATCCAACTAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGTTGTACTTCAGGATCGGACTGATGAGATTGTTGACAAGTTAGCAGAGAAAACTGAAAGTCTGTCAGTTTCTGATGGTTTGGAGACTACCACATTTTCTGGACTTAAAAAGAAGAAGAAAAAACAAACCATAAGGGAGTTATTCTACAGTTTCGTTATCCTTGGGAGGGAAGTGATTGTGTCACACCTTGCCCAGCACACGTCTTTGCTTGGTATGCGAGACACGGGCTTAATGGTTGGAGAGTCGAAAAATGGAATTCTTGGAGGGTTTGAAAGATCTCAAGTGAAGGTTTGGTCTTATGAATATGTGATTTGCATTGGGTGCAAGAGTCTGGACACTCTTCTTTCAAAGGAGAACCGGCTTTTCTTTCTCAGGTGCGAGATGTGGTTCTGGACGATCAGTTGCTCCAAT

Protein sequence

MEDEVQNDANDQVADIAFDPTKKKKKKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDGLETTTFSGLKKKKKKQTIRELFYSFVILGREVIVSHLAQHTSLLGMRDTGLMVGESKNGILGGFERSQVKVWSYEYVICIGCKSLDTLLSKENRLFFLRCEMWFWTISCSN

Homology

[Close]

BLAST of CSPI01G04860 vs. ExPASy Swiss-Prot
Match: P55871 (Eukaryotic translation initiation factor 2 subunit beta OS=Malus domestica OX=3750 PE=2 SV=2)

HSP 1 Score: 80.5 bits (197), Expect = 2.0e-14
Identity = 84/250 (33.60%), Postives = 106/250 (42.40%), Query Frame = 0


Query: 1   MEDEVQNDANDQ-VADIA-FDPTKKKKKKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDG 60
           M D+ QN+  D+ VADIA FDPT    KKKKKK+VV+QD TD+ V KLAEK E+ + S+G
Sbjct: 1   MADDNQNEVKDEVVADIAPFDPT----KKKKKKEVVIQDTTDDSVGKLAEKAEACTASEG 60

Query: 61  LETTTFSGLKKKKKKQTIRELF-------------------------------------- 120
            E +TF+GLKKKKKK     +                                       
Sbjct: 61  QE-STFAGLKKKKKKPIETSILNEESGDAVEDLNERTGEDEEGEGIVLETPSYPWEGSDR 120

Query: 121 -YSFVILGREVIVSHLAQHTSLLGMRDTGLM-------VGESK----------------- 160
            Y++  L   V       +  L G R   +M        G  K                 
Sbjct: 121 DYTYEELLDRVFTILRENNPDLAGDRRRTVMRPPQVLREGTKKAVFVNFMDLCKTMHRQP 180

[Close]

BLAST of CSPI01G04860 vs. ExPASy Swiss-Prot
Match: O24473 (Eukaryotic translation initiation factor 2 subunit beta OS=Triticum aestivum OX=4565 PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.0e-13
Identity = 83/252 (32.94%), Postives = 107/252 (42.46%), Query Frame = 0


Query: 1   MEDEVQNDANDQVADIA-FDPTKKKKKKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDGL 60
           M DE Q +  ++  +IA FDPT    KKKKKKKVV+QD  DE VDKLAEKTE LSV++  
Sbjct: 1   MADEEQMERKEEATEIAPFDPT----KKKKKKKVVIQDPADE-VDKLAEKTEGLSVTESG 60

Query: 61  ETTTFSGLKKKKKK---------------------------------------------- 120
           E  +F GLKKKKKK                                              
Sbjct: 61  E-ASFVGLKKKKKKLVELDPSLVEAGDGEDTLDDQVGEDEQGEGIVLGGATQYPWEGTDR 120

Query: 121 ----------------------------------QTIRE-----LFYSFVILGREVIVSH 160
                                             Q +RE     +F +F+ L +   +  
Sbjct: 121 DYKYDELLGRVFNILRENNPDLAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCK--TMHR 180

[Close]

BLAST of CSPI01G04860 vs. ExPASy Swiss-Prot
Match: Q41969 (Eukaryotic translation initiation factor 2 subunit beta OS=Arabidopsis thaliana OX=3702 GN=EIF2B PE=1 SV=3)

HSP 1 Score: 66.2 bits (160), Expect = 4.0e-10
Identity = 70/250 (28.00%), Postives = 106/250 (42.40%), Query Frame = 0


Query: 1   MEDEVQNDANDQVADIAFDPTKKKKKKKKKKKVVLQDRTDEIVD-KLAEKTESLSVSDGL 60
           M DE+     +Q     FDP+    KKKKKKKVV+Q+  +++ +    EK++SL V+DGL
Sbjct: 1   MADEINEIREEQEQLAPFDPS----KKKKKKKVVIQEPVEDLAESSQTEKSDSLPVNDGL 60

Query: 61  ETTTFSGLKKKKKK---------------------------------------------- 120
           E ++F+G+KKKKKK                                              
Sbjct: 61  E-SSFTGMKKKKKKPTESSLLNNESVDAGEDLDEIANDEQEGEEGIVLQQRYPWEGSERD 120

Query: 121 ---------------------------------QTIRE-----LFYSFVILGREV--IVS 160
                                            Q +RE     +F +F+ L + +     
Sbjct: 121 YIYDELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPD 180

[Close]

BLAST of CSPI01G04860 vs. ExPASy Swiss-Prot
Match: P56329 (Probable eukaryotic translation initiation factor 2 subunit beta OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tif212 PE=1 SV=2)

HSP 1 Score: 50.4 bits (119), Expect = 2.2e-05
Identity = 25/57 (43.86%), Postives = 37/57 (64.91%), Query Frame = 0


Query: 107 TGLMVGESKNGILGGFERSQV----KVWSYEYVICIGCKSLDTLLSKENRLFFLRCE 160
           +G + G S+  I G F++ Q+    + +  EYV C  CKS DT+L+KENR+FF+ CE
Sbjct: 239 SGSVDGSSRLIIKGRFQQKQIENVLRRYIVEYVTCKTCKSPDTILTKENRIFFMTCE 295

[Close]

BLAST of CSPI01G04860 vs. ExPASy Swiss-Prot
Match: Q5E9D0 (Eukaryotic translation initiation factor 2 subunit 2 OS=Bos taurus OX=9913 GN=EIF2S2 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 9.4e-04
Identity = 22/57 (38.60%), Postives = 36/57 (63.16%), Query Frame = 0


Query: 107 TGLMVGESKNGILGGFERSQV----KVWSYEYVICIGCKSLDTLLSKENRLFFLRCE 160
           +G + G ++  I G F++ Q+    + +  EYV C  C+S DT+L K+ RL+FL+CE
Sbjct: 247 SGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFLQCE 303

[Close]

BLAST of CSPI01G04860 vs. ExPASy TrEMBL
Match: A0A0A0LT22 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G028050 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 1.0e-24
Identity = 70/74 (94.59%), Postives = 70/74 (94.59%), Query Frame = 0


Query: 1  MEDEVQNDANDQVADIAFDPTKKKKKKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDGLE 60
          MEDEVQNDANDQVADIAFDPT    KKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDGLE
Sbjct: 1  MEDEVQNDANDQVADIAFDPT----KKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDGLE 60

Query: 61 TTTFSGLKKKKKKQ 75
          TTTFSGLKKKKKKQ
Sbjct: 61 TTTFSGLKKKKKKQ 70

[Close]

BLAST of CSPI01G04860 vs. ExPASy TrEMBL
Match: A0A1S3CG20 (eukaryotic translation initiation factor 2 subunit beta OS=Cucumis melo OX=3656 GN=LOC103500512 PE=3 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 1.3e-24
Identity = 102/250 (40.80%), Postives = 118/250 (47.20%), Query Frame = 0


Query: 1   MEDEVQNDANDQVADIA-FDPTKKKKKKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDGL 60
           M DEVQND  DQVADIA FDPT    KKKKKKKVVLQD TDEIVDKLAEKTESLSVSDGL
Sbjct: 1   MADEVQNDVTDQVADIAPFDPT----KKKKKKKVVLQDPTDEIVDKLAEKTESLSVSDGL 60

Query: 61  ETTTFSGLKKKKKK---------------------------------------------- 120
           ETTTFSGLKKKKKK                                              
Sbjct: 61  ETTTFSGLKKKKKKPVEASSLNEDSADATEDLEDQAGEIEDREGIVLQYRYPWEGTDRDY 120

Query: 121 --------------------------------QTIRE-----LFYSFVILGREVIVSHLA 160
                                           Q +RE     +F +F+ L +   +    
Sbjct: 121 EYEELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCK--TMHRQP 180

[Close]

BLAST of CSPI01G04860 vs. ExPASy TrEMBL
Match: A0A6J1HIQ4 (eukaryotic translation initiation factor 2 subunit beta-like OS=Cucurbita moschata OX=3662 GN=LOC111464801 PE=3 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 5.0e-24
Identity = 101/250 (40.40%), Postives = 117/250 (46.80%), Query Frame = 0


Query: 1   MEDEVQNDANDQVADIA-FDPTKKKKKKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDGL 60
           M DEVQND NDQVADIA FDPT    KKKKKKKVVLQD  DE VDKLAEKTESLSVSDGL
Sbjct: 1   MADEVQNDVNDQVADIAPFDPT----KKKKKKKVVLQDLADESVDKLAEKTESLSVSDGL 60

Query: 61  ETTTFSGLKKKKKK---------------------------------------------- 120
           ETTTFSGLKKKKKK                                              
Sbjct: 61  ETTTFSGLKKKKKKPVEAISLTDDGADATEDLEDQAGENEDQDGITLQYRYPWEGSDRDY 120

Query: 121 --------------------------------QTIRE-----LFYSFVILGREVIVSHLA 160
                                           Q +RE     +F +F+ L +   +    
Sbjct: 121 EYEELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCK--TMHRQP 180

[Close]

BLAST of CSPI01G04860 vs. ExPASy TrEMBL
Match: A0A6J1HZQ9 (eukaryotic translation initiation factor 2 subunit beta-like OS=Cucurbita maxima OX=3661 GN=LOC111469574 PE=3 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.9e-23
Identity = 100/251 (39.84%), Postives = 116/251 (46.22%), Query Frame = 0


Query: 1   MEDEVQNDANDQVADIA--FDPTKKKKKKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDG 60
           M DE QNDANDQVADI   FDPT    KKKKKKKVVLQD  DE VDKLAEKTESLSVSDG
Sbjct: 1   MADEAQNDANDQVADIVAPFDPT----KKKKKKKVVLQDLADESVDKLAEKTESLSVSDG 60

Query: 61  LETTTFSGLKKKKKK--------------------------------------------- 120
           LETTTFSGLKKKKKK                                             
Sbjct: 61  LETTTFSGLKKKKKKPVEAISLNDDGADATEDLEDQAGENEDQDGITLQYRYPWEGSDRD 120

Query: 121 ---------------------------------QTIRE-----LFYSFVILGREVIVSHL 160
                                            Q +RE     +F +F+ L +   +   
Sbjct: 121 YEYEELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCK--TMHRQ 180

[Close]

BLAST of CSPI01G04860 vs. ExPASy TrEMBL
Match: A0A0A0LY41 (eIF2B_5 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G145930 PE=3 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.9e-23
Identity = 100/250 (40.00%), Postives = 116/250 (46.40%), Query Frame = 0


Query: 1   MEDEVQNDANDQVADIA-FDPTKKKKKKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDGL 60
           M DEVQND NDQVADIA FDPT    KKKKKKKVVLQD TDE VDKLAEKTESLSVSDGL
Sbjct: 1   MADEVQNDVNDQVADIAPFDPT----KKKKKKKVVLQDPTDESVDKLAEKTESLSVSDGL 60

Query: 61  ETTTFSGLKKKKKK---------------------------------------------- 120
           E  TFSGLKKKKKK                                              
Sbjct: 61  EAATFSGLKKKKKKPVEASSLNEDGADATEDLEDQAGENEDREGLVLQYRYPWEGTDRDY 120

Query: 121 --------------------------------QTIRE-----LFYSFVILGREVIVSHLA 160
                                           Q +RE     +F +F+ L +   +    
Sbjct: 121 EYEELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCK--TMHRQP 180

[Close]

BLAST of CSPI01G04860 vs. NCBI nr
Match: KGN63931.2 (hypothetical protein Csa_014332 [Cucumis sativus])

HSP 1 Score: 151.8 bits (382), Expect = 5.5e-33
Identity = 94/128 (73.44%), Postives = 98/128 (76.56%), Query Frame = 0


Query: 1   MEDEVQNDANDQVADIAFDPTKKKKKKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDGLE 60
           MEDEVQNDANDQVADIAFDPT    KKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDGLE
Sbjct: 1   MEDEVQNDANDQVADIAFDPT----KKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDGLE 60

Query: 61  TTTFSGLKKKKKKQTIRELFYSFVILGREVIVSHLAQHTSLLGMRDTGLMVGESKNGILG 120
           TTTFSGLKKKKKKQ   E+  ++ I    +I                GLMVGESKNGILG
Sbjct: 61  TTTFSGLKKKKKKQV--EVSNAYGICKHNLI---------------QGLMVGESKNGILG 107

Query: 121 GFERSQVK 129
           GFERSQVK
Sbjct: 121 GFERSQVK 107

[Close]

BLAST of CSPI01G04860 vs. NCBI nr
Match: XP_038894246.1 (eukaryotic translation initiation factor 2 subunit beta [Benincasa hispida] >XP_038894247.1 eukaryotic translation initiation factor 2 subunit beta [Benincasa hispida])

HSP 1 Score: 125.2 bits (313), Expect = 5.5e-25
Identity = 103/250 (41.20%), Postives = 119/250 (47.60%), Query Frame = 0


Query: 1   MEDEVQNDANDQVADIA-FDPTKKKKKKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDGL 60
           M DEVQND NDQVADIA FDPT    KKKKKKKVVLQD TDEIVDKLAEKTESLSVSDGL
Sbjct: 1   MADEVQNDVNDQVADIAPFDPT----KKKKKKKVVLQDPTDEIVDKLAEKTESLSVSDGL 60

Query: 61  ETTTFSGLKKKKKK---------------------------------------------- 120
           ETTTFSGLKKKKKK                                              
Sbjct: 61  ETTTFSGLKKKKKKPVEASSLNDDGADGTEDLEDQVGENEDREGIVLQYRYPWEGSDRDY 120

Query: 121 --------------------------------QTIRE-----LFYSFVILGREVIVSHLA 160
                                           Q +RE     +F +F+ L +   +    
Sbjct: 121 EYEELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCK--TMHRQP 180

[Close]

BLAST of CSPI01G04860 vs. NCBI nr
Match: XP_008462075.1 (PREDICTED: eukaryotic translation initiation factor 2 subunit beta [Cucumis melo] >XP_008462082.1 PREDICTED: eukaryotic translation initiation factor 2 subunit beta [Cucumis melo])

HSP 1 Score: 122.9 bits (307), Expect = 2.7e-24
Identity = 102/250 (40.80%), Postives = 118/250 (47.20%), Query Frame = 0


Query: 1   MEDEVQNDANDQVADIA-FDPTKKKKKKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDGL 60
           M DEVQND  DQVADIA FDPT    KKKKKKKVVLQD TDEIVDKLAEKTESLSVSDGL
Sbjct: 1   MADEVQNDVTDQVADIAPFDPT----KKKKKKKVVLQDPTDEIVDKLAEKTESLSVSDGL 60

Query: 61  ETTTFSGLKKKKKK---------------------------------------------- 120
           ETTTFSGLKKKKKK                                              
Sbjct: 61  ETTTFSGLKKKKKKPVEASSLNEDSADATEDLEDQAGEIEDREGIVLQYRYPWEGTDRDY 120

Query: 121 --------------------------------QTIRE-----LFYSFVILGREVIVSHLA 160
                                           Q +RE     +F +F+ L +   +    
Sbjct: 121 EYEELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCK--TMHRQP 180

[Close]

BLAST of CSPI01G04860 vs. NCBI nr
Match: KAG7018698.1 (hypothetical protein SDJN02_20569, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 120.9 bits (302), Expect = 1.0e-23
Identity = 101/250 (40.40%), Postives = 117/250 (46.80%), Query Frame = 0


Query: 1   MEDEVQNDANDQVADIA-FDPTKKKKKKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDGL 60
           M DEVQND NDQVADIA FDPT    KKKKKKKVVLQD  DE VDKLAEKTESLSVSDGL
Sbjct: 79  MADEVQNDVNDQVADIAPFDPT----KKKKKKKVVLQDLADESVDKLAEKTESLSVSDGL 138

Query: 61  ETTTFSGLKKKKKK---------------------------------------------- 120
           ETTTFSGLKKKKKK                                              
Sbjct: 139 ETTTFSGLKKKKKKPVEAISLTDDGADATEDLEDQAGENEDQDGITLQYRYPWEGSDRDY 198

Query: 121 --------------------------------QTIRE-----LFYSFVILGREVIVSHLA 160
                                           Q +RE     +F +F+ L +   +    
Sbjct: 199 EYEELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCK--TMHRQP 258

[Close]

BLAST of CSPI01G04860 vs. NCBI nr
Match: KAG6583311.1 (hypothetical protein SDJN03_19243, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 120.9 bits (302), Expect = 1.0e-23
Identity = 101/250 (40.40%), Postives = 117/250 (46.80%), Query Frame = 0


Query: 1   MEDEVQNDANDQVADIA-FDPTKKKKKKKKKKKVVLQDRTDEIVDKLAEKTESLSVSDGL 60
           M DEVQND NDQVADIA FDPT    KKKKKKKVVLQD  DE VDKLAEKTESLSVSDGL
Sbjct: 78  MADEVQNDVNDQVADIAPFDPT----KKKKKKKVVLQDLADESVDKLAEKTESLSVSDGL 137

Query: 61  ETTTFSGLKKKKKK---------------------------------------------- 120
           ETTTFSGLKKKKKK                                              
Sbjct: 138 ETTTFSGLKKKKKKPVEAISLTDDGADATEDLEDQAGENEDQDGITLQYRYPWEGSDRDY 197

Query: 121 --------------------------------QTIRE-----LFYSFVILGREVIVSHLA 160
                                           Q +RE     +F +F+ L +   +    
Sbjct: 198 EYEELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCK--TMHRQP 257

[Close]

BLAST of CSPI01G04860 vs. TAIR 10
Match: AT5G20920.1 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 66.2 bits (160), Expect = 2.8e-11
Identity = 70/250 (28.00%), Postives = 106/250 (42.40%), Query Frame = 0


Query: 1   MEDEVQNDANDQVADIAFDPTKKKKKKKKKKKVVLQDRTDEIVD-KLAEKTESLSVSDGL 60
           M DE+     +Q     FDP+    KKKKKKKVV+Q+  +++ +    EK++SL V+DGL
Sbjct: 1   MADEINEIREEQEQLAPFDPS----KKKKKKKVVIQEPVEDLAESSQTEKSDSLPVNDGL 60

Query: 61  ETTTFSGLKKKKKK---------------------------------------------- 120
           E ++F+G+KKKKKK                                              
Sbjct: 61  E-SSFTGMKKKKKKPTESSLLNNESVDAGEDLDEIANDEQEGEEGIVLQQRYPWEGSERD 120

Query: 121 ---------------------------------QTIRE-----LFYSFVILGREV--IVS 160
                                            Q +RE     +F +F+ L + +     
Sbjct: 121 YIYDELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPD 180

[Close]

BLAST of CSPI01G04860 vs. TAIR 10
Match: AT5G20920.3 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 66.2 bits (160), Expect = 2.8e-11
Identity = 70/250 (28.00%), Postives = 106/250 (42.40%), Query Frame = 0


Query: 1   MEDEVQNDANDQVADIAFDPTKKKKKKKKKKKVVLQDRTDEIVD-KLAEKTESLSVSDGL 60
           M DE+     +Q     FDP+    KKKKKKKVV+Q+  +++ +    EK++SL V+DGL
Sbjct: 1   MADEINEIREEQEQLAPFDPS----KKKKKKKVVIQEPVEDLAESSQTEKSDSLPVNDGL 60

Query: 61  ETTTFSGLKKKKKK---------------------------------------------- 120
           E ++F+G+KKKKKK                                              
Sbjct: 61  E-SSFTGMKKKKKKPTESSLLNNESVDAGEDLDEIANDEQEGEEGIVLQQRYPWEGSERD 120

Query: 121 ---------------------------------QTIRE-----LFYSFVILGREV--IVS 160
                                            Q +RE     +F +F+ L + +     
Sbjct: 121 YIYDELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPD 180

[Close]

BLAST of CSPI01G04860 vs. TAIR 10
Match: AT5G20920.2 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 64.7 bits (156), Expect = 8.2e-11
Identity = 70/250 (28.00%), Postives = 108/250 (43.20%), Query Frame = 0


Query: 1   MEDEVQNDANDQVADIAFDPTKKKKKKKKKKKVVLQDRTDEIVD-KLAEKTESLSVSDGL 60
           M DE+ N+  ++     FDP+    KKKKKKKVV+Q+  +++ +    EK++SL V+DGL
Sbjct: 1   MADEI-NEIREEQELAPFDPS----KKKKKKKVVIQEPVEDLAESSQTEKSDSLPVNDGL 60

Query: 61  ETTTFSGLKKKKKK---------------------------------------------- 120
           E ++F+G+KKKKKK                                              
Sbjct: 61  E-SSFTGMKKKKKKPTESSLLNNESVDAGEDLDEIANDEQEGEEGIVLQQRYPWEGSERD 120

Query: 121 ---------------------------------QTIRE-----LFYSFVILGREV--IVS 160
                                            Q +RE     +F +F+ L + +     
Sbjct: 121 YIYDELLGRVFNILRENNPELAGDRRRTVMRPPQVLREGTKKTVFVNFMDLCKTMHRQPD 180

[Close]

BLAST of CSPI01G04860 vs. TAIR 10
Match: AT3G07920.1 (Translation initiation factor IF2/IF5 )

HSP 1 Score: 47.8 bits (112), Expect = 1.0e-05
Identity = 20/34 (58.82%), Postives = 27/34 (79.41%), Query Frame = 0


Query: 125 SQVKVWSYEYVICIGCKSLDTLLSKENRLFFLRC 159
           S ++ +  +YV+CI CKS + +LSKENRLFFLRC
Sbjct: 112 SLLRGYILDYVMCIPCKSTEAILSKENRLFFLRC 145

The following BLAST results are available for this feature:

BLAST of CSPI01G04860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P55871	2.0e-14	33.60	Eukaryotic translation initiation factor 2 subunit beta OS=Malus domestica OX=37...	[more]
O24473	5.0e-13	32.94	Eukaryotic translation initiation factor 2 subunit beta OS=Triticum aestivum OX=...	[more]
Q41969	4.0e-10	28.00	Eukaryotic translation initiation factor 2 subunit beta OS=Arabidopsis thaliana ...	[more]
P56329	2.2e-05	43.86	Probable eukaryotic translation initiation factor 2 subunit beta OS=Schizosaccha...	[more]
Q5E9D0	9.4e-04	38.60	Eukaryotic translation initiation factor 2 subunit 2 OS=Bos taurus OX=9913 GN=EI...	[more]

BLAST of CSPI01G04860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LT22	1.0e-24	94.59	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G028050 PE=4 SV=1	[more]
A0A1S3CG20	1.3e-24	40.80	eukaryotic translation initiation factor 2 subunit beta OS=Cucumis melo OX=3656 ...	[more]
A0A6J1HIQ4	5.0e-24	40.40	eukaryotic translation initiation factor 2 subunit beta-like OS=Cucurbita moscha...	[more]
A0A6J1HZQ9	1.9e-23	39.84	eukaryotic translation initiation factor 2 subunit beta-like OS=Cucurbita maxima...	[more]
A0A0A0LY41	1.9e-23	40.00	eIF2B_5 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G145930 PE=...	[more]

BLAST of CSPI01G04860 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN63931.2	5.5e-33	73.44	hypothetical protein Csa_014332 [Cucumis sativus]	[more]
XP_038894246.1	5.5e-25	41.20	eukaryotic translation initiation factor 2 subunit beta [Benincasa hispida] >XP_...	[more]
XP_008462075.1	2.7e-24	40.80	PREDICTED: eukaryotic translation initiation factor 2 subunit beta [Cucumis melo...	[more]
KAG7018698.1	1.0e-23	40.40	hypothetical protein SDJN02_20569, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6583311.1	1.0e-23	40.40	hypothetical protein SDJN03_19243, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of CSPI01G04860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20920.1	2.8e-11	28.00	eukaryotic translation initiation factor 2 beta subunit	[more]
AT5G20920.3	2.8e-11	28.00	eukaryotic translation initiation factor 2 beta subunit	[more]
AT5G20920.2	8.2e-11	28.00	eukaryotic translation initiation factor 2 beta subunit	[more]
AT3G07920.1	1.0e-05	58.82	Translation initiation factor IF2/IF5	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.30.170		coord: 115..163 e-value: 6.0E-6 score: 27.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..30
None	No IPR available	PANTHER	PTHR23001	EUKARYOTIC TRANSLATION INITIATION FACTOR	coord: 1..69 coord: 129..160
None	No IPR available	PANTHER	PTHR23001:SF25	EUKARYOTIC TRANSLATION INITIATION FACTOR 2 BETA SUBUNIT	coord: 129..160
None	No IPR available	PANTHER	PTHR23001:SF25	EUKARYOTIC TRANSLATION INITIATION FACTOR 2 BETA SUBUNIT	coord: 1..69
IPR002735	Translation initiation factor IF2/IF5	PFAM	PF01873	eIF-5_eIF-2B	coord: 130..159 e-value: 1.5E-5 score: 24.9
IPR016190	Translation initiation factor IF2/IF5, zinc-binding	SUPERFAMILY	75689	Zinc-binding domain of translation initiation factor 2 beta	coord: 134..160

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G04860.1	CSPI01G04860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018105	peptidyl-serine phosphorylation
biological_process	GO:0018107	peptidyl-threonine phosphorylation
biological_process	GO:0006413	translational initiation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004713	protein tyrosine kinase activity
molecular_function	GO:0003743	translation initiation factor activity