Bhi11G001019 (gene) Wax gourd (B227) v1

Overview
NameBhi11G001019
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionEpoxide hydrolase 2
Locationchr11: 35765927 .. 35767480 (-)
RNA-Seq ExpressionBhi11G001019
SyntenyBhi11G001019
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCCAAATGAGGCCATCCCCACATACACACACACAAAAAACCTTCCTATTTTAACTCAACCTAGAGACAGAGATAATGAACAAAAACAAAACTCATCAATGAACAAGATCCAACACTCAACCATCCCCACCAATGGCATTAACATCCACGTCGCTTCCATTGGTTCCGGTCCGGCGGTTCTATTTCTCCACGGCTTCCCGGAGCTCTGGTACTCATGGCGCCACCAGCTCCTCTTTCTCGCTTCCAAAGGCTTCCGAGCCATAGCCCCTGACCTCCGCGGCTTCGGCGACTCCGATGTCCTGCCGTCTCCCTCCTCCTATACCCCTCACCACATCGTCGGCGACCTCATTGGCCTCCTCGATCATCTCGGAATCGATCAAGTTTTCTTGGTTGGCCACGATTGGGGCGCTATGATGGCTTGGTACTTTTGCCTTTTCAGGCCAGATCGAGTCAAAGCTTTGGTCAACTTGAGTGTCCATTACACTCCTCGGAATCCGTCAATCTCGCCTCTCGCCGGCGTCCGCCTGTTGCTCGGCGATAATTTCTACTTTTGTAAATTTCAGGTGAAGCCGATTGAATCTTTGAACTCTTTTGTAATCTCATTAGGTCAACTGACGAATCGCTAATTGGGAATGTAATCCCTGAAATTGCAAGAGGCGAATCGCATTAAATGAACTGATTAATCGGAAATTGTGAATTTGGGAACAGGAACCTGGAGTAGCGGAAGCCGATTTTGGATCTGTAGATACGGCTACAATGATGAAGAAATTCCTGACGATGAGAGATCCAAGCCCTCCGATTATACCTAATGGATTTAAAACACTAGCGACTCCAGAAACATTACCTCCATGGTTAACAGAAGAAGATATCGATTATTTCGCCTCCAAATTCGGCAAAACTGGCTTCACAGGCGGATTCAACTACTATCGTGCTACTGACTTGTGAGGATTCACAAATTTCGATTTCCCTGTTGTGTTATGGATTATTTAAGAACCGATATTGTGTGTTTTGTTGATTCATTGCAGAAGTTGGGAGCTTACAGGGCCATGGACTGGGGCACAGATCAAAGTTCCTACGAAATTCATAGTGGGGGATCTGGATATGGTGTACCATTTACCAGGTGCGAAGGAGTATATTCATGGCGGCGGATTCAAGAAGAAAGTACCGCTTCTGGAAGAAGTGGTGGTGATAGAAGGAGCAGCTCACTTCATTAACCAAGAAAAAGCATATGATATAAGCTCCCTCATCTATGATTTCATCAAAAAGTTCTGAATTTCTGAATATTTCTCTGCTTTTCCTTTCCATGCGGAAGTTCAATGACAAACAACTCTTCAACATCATATCATATCTGTTGTGGTATGGATATTCAAGCAGATTTTATGTTTTCAGATTTATTAAATTTAGCGAATATAGACCGCATTCAATTTCTGAAAATTGTTTTAAGATTATGTGATTCAAATAAGGGATTCAGAGGTTGAATTTTAAAATGTTGAATTATAGTTAAAAAAAAACATTAATAAATATAACATTTCATGTTATAAATTTAATTATTTT

mRNA sequence

TTCCAAATGAGGCCATCCCCACATACACACACACAAAAAACCTTCCTATTTTAACTCAACCTAGAGACAGAGATAATGAACAAAAACAAAACTCATCAATGAACAAGATCCAACACTCAACCATCCCCACCAATGGCATTAACATCCACGTCGCTTCCATTGGTTCCGGTCCGGCGGTTCTATTTCTCCACGGCTTCCCGGAGCTCTGGTACTCATGGCGCCACCAGCTCCTCTTTCTCGCTTCCAAAGGCTTCCGAGCCATAGCCCCTGACCTCCGCGGCTTCGGCGACTCCGATGTCCTGCCGTCTCCCTCCTCCTATACCCCTCACCACATCGTCGGCGACCTCATTGGCCTCCTCGATCATCTCGGAATCGATCAAGTTTTCTTGGTTGGCCACGATTGGGGCGCTATGATGGCTTGGTACTTTTGCCTTTTCAGGCCAGATCGAGTCAAAGCTTTGGTCAACTTGAGTGTCCATTACACTCCTCGGAATCCGTCAATCTCGCCTCTCGCCGGCGTCCGCCTGTTGCTCGGCGATAATTTCTACTTTTGTAAATTTCAGGAACCTGGAGTAGCGGAAGCCGATTTTGGATCTGTAGATACGGCTACAATGATGAAGAAATTCCTGACGATGAGAGATCCAAGCCCTCCGATTATACCTAATGGATTTAAAACACTAGCGACTCCAGAAACATTACCTCCATGGTTAACAGAAGAAGATATCGATTATTTCGCCTCCAAATTCGGCAAAACTGGCTTCACAGGCGGATTCAACTACTATCGTGCTACTGACTTAAGTTGGGAGCTTACAGGGCCATGGACTGGGGCACAGATCAAAGTTCCTACGAAATTCATAGTGGGGGATCTGGATATGGTGTACCATTTACCAGGTGCGAAGGAGTATATTCATGGCGGCGGATTCAAGAAGAAAGTACCGCTTCTGGAAGAAGTGGTGGTGATAGAAGGAGCAGCTCACTTCATTAACCAAGAAAAAGCATATGATATAAGCTCCCTCATCTATGATTTCATCAAAAAGTTCTGAATTTCTGAATATTTCTCTGCTTTTCCTTTCCATGCGGAAGTTCAATGACAAACAACTCTTCAACATCATATCATATCTGTTGTGGTATGGATATTCAAGCAGATTTTATGTTTTCAGATTTATTAAATTTAGCGAATATAGACCGCATTCAATTTCTGAAAATTGTTTTAAGATTATGTGATTCAAATAAGGGATTCAGAGGTTGAATTTTAAAATGTTGAATTATAGTTAAAAAAAAACATTAATAAATATAACATTTCATGTTATAAATTTAATTATTTT

Coding sequence (CDS)

ATGAACAAGATCCAACACTCAACCATCCCCACCAATGGCATTAACATCCACGTCGCTTCCATTGGTTCCGGTCCGGCGGTTCTATTTCTCCACGGCTTCCCGGAGCTCTGGTACTCATGGCGCCACCAGCTCCTCTTTCTCGCTTCCAAAGGCTTCCGAGCCATAGCCCCTGACCTCCGCGGCTTCGGCGACTCCGATGTCCTGCCGTCTCCCTCCTCCTATACCCCTCACCACATCGTCGGCGACCTCATTGGCCTCCTCGATCATCTCGGAATCGATCAAGTTTTCTTGGTTGGCCACGATTGGGGCGCTATGATGGCTTGGTACTTTTGCCTTTTCAGGCCAGATCGAGTCAAAGCTTTGGTCAACTTGAGTGTCCATTACACTCCTCGGAATCCGTCAATCTCGCCTCTCGCCGGCGTCCGCCTGTTGCTCGGCGATAATTTCTACTTTTGTAAATTTCAGGAACCTGGAGTAGCGGAAGCCGATTTTGGATCTGTAGATACGGCTACAATGATGAAGAAATTCCTGACGATGAGAGATCCAAGCCCTCCGATTATACCTAATGGATTTAAAACACTAGCGACTCCAGAAACATTACCTCCATGGTTAACAGAAGAAGATATCGATTATTTCGCCTCCAAATTCGGCAAAACTGGCTTCACAGGCGGATTCAACTACTATCGTGCTACTGACTTAAGTTGGGAGCTTACAGGGCCATGGACTGGGGCACAGATCAAAGTTCCTACGAAATTCATAGTGGGGGATCTGGATATGGTGTACCATTTACCAGGTGCGAAGGAGTATATTCATGGCGGCGGATTCAAGAAGAAAGTACCGCTTCTGGAAGAAGTGGTGGTGATAGAAGGAGCAGCTCACTTCATTAACCAAGAAAAAGCATATGATATAAGCTCCCTCATCTATGATTTCATCAAAAAGTTCTGA

Protein sequence

MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLRGFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKALVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMRDPSPPIIPNGFKTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTGPWTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEKAYDISSLIYDFIKKF
Homology
BLAST of Bhi11G001019 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 452.6 bits (1163), Expect = 2.6e-127
Identity = 201/316 (63.61%), Postives = 246/316 (77.85%), Query Frame = 0

Query: 1   MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           M KI+H+TI TNGIN+HVASIGSGP +LF+HGFP+LWYSWRHQL+  A+ G+RAIAPDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 61  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120
           G+GDSD  PS  SYT  HIVGDL+GLLD LG+D+VFLVGHDWGA++AW+ C+ RPDRV A
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNA 120

Query: 121 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 180
           LVN SV + PRNPS+ P+   R L GD++Y C+FQEPG  E DF  VDT  ++ +F T R
Sbjct: 121 LVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFTSR 180

Query: 181 DPSPPIIPN--GFKTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELT 240
           +P PP IP   GF+ L  P +LP WLTE+D+ ++  KF + GFTGG NYYRA +LSWELT
Sbjct: 181 NPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWELT 240

Query: 241 GPWTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQE 300
            PWTG QIKVP KFIVGDLD+ Y++PG KEYIH GG KK VP L+EVVV+EG  HF++QE
Sbjct: 241 APWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQE 300

Query: 301 KAYDISSLIYDFIKKF 315
           K  +++  IY F KKF
Sbjct: 301 KPDEVTDHIYGFFKKF 316

BLAST of Bhi11G001019 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 373.2 bits (957), Expect = 2.0e-103
Identity = 183/323 (56.66%), Postives = 231/323 (71.52%), Query Frame = 0

Query: 4   IQHSTIPTNGINIHVASIG--SGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLRG 63
           ++H  +  NGI+IHVA  G   GP VL LHGFPELWYSWRHQ+  LA++G+RA+APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  FGDSDVLPSPSSYTPHHIVGDLIGLLDHLGI---DQVFLVGHDWGAMMAWYFCLFRPDRV 123
           +GDSD     SSYT  +IVGDLI ++  L     ++VF+VGHDWGA++AWY CLFRPDRV
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 KALVNLSVHYT--PRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKF 183
           KALVNLSV ++  P +PS+ P+  +R   GD++Y C+FQE G  EA+   V T  +MK+ 
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 LTMRDPSPPIIPN-----GFKTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRAT 243
           LT R P P IIP      G K    P  LP WLTEED+ YF SKF + GF+G  NYYR  
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIP--LPSWLTEEDVAYFVSKFEEKGFSGPVNYYRNF 240

Query: 244 DLSWELTGPWTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGA 303
           + + EL GPW G++I+VPTKF++G+LD+VY++PG KEYIHG  FK+ VPLLEE VV+EG 
Sbjct: 241 NRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGV 300

Query: 304 AHFINQEKAYDISSLIYDFIKKF 315
           AHFINQEK  +I  +I DFI KF
Sbjct: 301 AHFINQEKPQEILQIILDFISKF 321

BLAST of Bhi11G001019 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 370.5 bits (950), Expect = 1.3e-102
Identity = 170/323 (52.63%), Postives = 220/323 (68.11%), Query Frame = 0

Query: 1   MNKIQHSTIPTNGINIHVASIG--SGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPD 60
           M  I H  +  NGI +H+A  G   GP VL LHGFP+LWY+WRHQ+  L+S G+RA+APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQ--VFLVGHDWGAMMAWYFCLFRPD 120
           LRG+GDSD   S S YT  ++VGDL+ LLD +  +Q  VFLVGHDWGA++ W+ CLFRP+
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 RVKALVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKF 180
           ++   V LSV Y  RNP + P+ G + + GD++Y C+FQEPG  E +  S D    ++  
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 LTMRDPSPPIIP--NGFKTLATPET----LPPWLTEEDIDYFASKFGKTGFTGGFNYYRA 240
            T R   PPI+P  N F     P +    LP W +++D+D++ SKF K GFTGG NYYRA
Sbjct: 181 FTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYRA 240

Query: 241 TDLSWELTGPWTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEG 300
            DL+WELT PWTGA+I+VP KF+ GD DMVY  PG KEYIHGGGF   VP L+E+VVIE 
Sbjct: 241 MDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIED 300

Query: 301 AAHFINQEKAYDISSLIYDFIKK 314
           A HF+NQEK  ++++ I DF  K
Sbjct: 301 AGHFVNQEKPQEVTAHINDFFTK 323

BLAST of Bhi11G001019 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 359.4 bits (921), Expect = 3.0e-99
Identity = 175/322 (54.35%), Postives = 228/322 (70.81%), Query Frame = 0

Query: 4   IQHSTIPTNGINIHVASIG--SGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLRG 63
           ++H  +  NGI+IHVA  G   G  VL LHGFPELWYSWRHQ+  LA++G+RA+APDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 64  FGDSDVLPSPSSYTPHHIVGDLIGLLDHLGID--QVFLVGHDWGAMMAWYFCLFRPDRVK 123
           +GDSD     SS+T  +IVGDL+ ++  L  +  +VF+VGHDWGA++AWY CLFRPD+VK
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 124 ALVNLSV--HYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFL 183
           ALVNLSV   + P +PS+ P+  +R + G+++Y C+FQE G  EA+   V T  +MK+ L
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 184 TMRDPSPPIIPN-----GFKTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATD 243
           T R P P IIP      G K    P  LP WLTEED+ YF SKF + GF G  NYYR  +
Sbjct: 181 TYRTPGPLIIPKDKSFWGSKGETIP--LPSWLTEEDVAYFVSKFKEKGFCGPVNYYRNFN 240

Query: 244 LSWELTGPWTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAA 303
            + EL GPW G++I+VPTKF++G+LD+VY++PG KEYIHG  FK+ VPL+EE VV+EG A
Sbjct: 241 RNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVA 300

Query: 304 HFINQEKAYDISSLIYDFIKKF 315
           HF+NQEK  +I  +I DFI  F
Sbjct: 301 HFLNQEKPQEILQIILDFISTF 320

BLAST of Bhi11G001019 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 349.4 bits (895), Expect = 3.1e-96
Identity = 159/324 (49.07%), Postives = 225/324 (69.44%), Query Frame = 0

Query: 1   MNKIQHSTIPTNGINIHVA-----SIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAI 60
           ++ ++H T+  NGIN+HVA       G  P +LFLHGFPELWY+WRHQ++ L+S G+R I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGFGDSDVLPSPSSYTPHHIVGDLIGLLDHL--GIDQVFLVGHDWGAMMAWYFCLF 120
           APDLRG+GD++       YT  ++ GD++ L+D +  G   V +VGHDWGAM+AW  C +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RPDRVKALVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMM 180
           RP++VKALVN+SV ++PRNP   P+  +R + GD++Y C+FQ+ G  E +F  + T  ++
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 KKFLTMRDPSPPIIPNG--FK-TLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRA 240
           K+FLT + P P  +P    FK +      LP WLT+ED+DY+ +K+   GFTG  NYYR 
Sbjct: 231 KEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYRN 290

Query: 241 TDLSWELTGPWTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEG 300
            D +WELT PWTGA+I+VP KFI+GD D+ Y+ PGAKEYI+GGGFK+ VPLL+E VV++G
Sbjct: 291 IDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKG 350

Query: 301 AAHFINQEKAYDISSLIYDFIKKF 315
             HF+++E    I+  I++F  KF
Sbjct: 351 LGHFLHEENPDVINQHIHNFFHKF 374

BLAST of Bhi11G001019 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 4.0e-56
Identity = 120/318 (37.74%), Postives = 170/318 (53.46%), Query Frame = 0

Query: 9   IPTNGINIHVASIG--SGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLRGFGDSD 68
           + TNG+ + V   G    P V+  HGFPEL YSWRHQ+  LA  G+  +APD RG+G S 
Sbjct: 9   VDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSS 68

Query: 69  VLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKALVNLSV 128
              +  +Y  H +  DL+GLLD +G ++   VGHDWGA++ W   L   DRV A+  LSV
Sbjct: 69  RPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALSV 128

Query: 129 HYTPRNPSISPLAGVRLLLGDN-FYFCKFQEPGVAEADFGSVDTATMMKKFLTMRDPSPP 188
              PR   + P    R   G+N FY   FQEPG+A+A+       TM +    +R P   
Sbjct: 129 PALPR-AQVPPTQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLRPPGDQ 188

Query: 189 II--------PNGF-KTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWE 248
                     P+GF   L  P  LP W+++E++D++  +F +TGFTGG N+YR  D +WE
Sbjct: 189 SAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNWYRNFDRNWE 248

Query: 249 LTGPWTGAQIKVPTKFIVGDLDMVY---HLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAH 308
            T    G  I VP+ FI G  D V        A E I G       P  E  V+I+GA H
Sbjct: 249 TTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVISG-------PYRE--VLIDGAGH 308

Query: 309 FINQEKAYDISSLIYDFI 312
           ++ QE+  ++++ + +F+
Sbjct: 309 WLQQERPGEVTAALLEFL 316

BLAST of Bhi11G001019 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 196.1 bits (497), Expect = 6.1e-49
Identity = 107/319 (33.54%), Postives = 169/319 (52.98%), Query Frame = 0

Query: 2   NKIQHSTIPTN-GINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 61
           N + H  +    GI +H   +GSGPA+   HGFPE W+SWR+Q+  LA  GFR +A D++
Sbjct: 233 NDVSHGYVTVKPGIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMK 292

Query: 62  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 121
           G+GDS   P    Y    +  +++  LD LGI Q   +GHDW  +M W   LF P+RV+A
Sbjct: 293 GYGDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRA 352

Query: 122 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 181
           + +L+  + P +P +SP+  +R +   N Y   FQEPGVAEA+    + +   K F    
Sbjct: 353 VASLNTPFMPPDPDVSPMKVIRSIPVFN-YQLYFQEPGVAEAEL-EKNMSRTFKSFFRAS 412

Query: 182 DPSPPIIPN-----GFKTLATPE--TLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDL 241
           D +  I  +     G   + TPE   L    TEE+I+++  +F KTGF G  N+YR T+ 
Sbjct: 413 DETGFIAVHKATEIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTER 472

Query: 242 SWELTGPWTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAH 301
           +W+ +    G +I VP   +  + D+V     +K        +K +P L+    IE   H
Sbjct: 473 NWKWSCKGLGRKILVPALMVTAEKDIVLRPEMSK------NMEKWIPFLKR-GHIEDCGH 532

Query: 302 FINQEKAYDISSLIYDFIK 313
           +   EK  +++ ++  +++
Sbjct: 533 WTQIEKPTEVNQILIKWLQ 542

BLAST of Bhi11G001019 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.4e-48
Identity = 105/318 (33.02%), Postives = 165/318 (51.89%), Query Frame = 0

Query: 2   NKIQHSTIPTN-GINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 61
           N + H  +    GI +H   +GSGPA+   HGFPE W+SWR+Q+  LA  GFR +A D++
Sbjct: 233 NDVSHGYVTVKPGIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMK 292

Query: 62  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 121
           G+GDS   P    Y    +  +++  L+ LGI Q   +GHDW  ++ W   LF P+RV+A
Sbjct: 293 GYGDSSSPPEIEEYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRA 352

Query: 122 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 181
           + +L+    P NP +SP+  +R +   N Y   FQEPGVAEA+     + T    F T  
Sbjct: 353 VASLNTPLMPPNPEVSPMEVIRSIPVFN-YQLYFQEPGVAEAELEKNMSRTFKSFFRTSD 412

Query: 182 DPSPPIIPN----GFKTLATPE--TLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLS 241
           D     +      G   + TPE   +    TEE+I+Y+  +F K+GF G  N+YR T+ +
Sbjct: 413 DMGLLTVNKATEMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERN 472

Query: 242 WELTGPWTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHF 301
           W+ +    G +I VP   +  + D+V     +K        +  +P L+    IE   H+
Sbjct: 473 WKWSCKALGRKILVPALMVTAEKDIVLRPEMSK------NMENWIPFLKR-GHIEDCGHW 532

Query: 302 INQEKAYDISSLIYDFIK 313
              EK  +++ ++  ++K
Sbjct: 533 TQIEKPAEVNQILIKWLK 542

BLAST of Bhi11G001019 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 187.6 bits (475), Expect = 2.2e-46
Identity = 97/304 (31.91%), Postives = 163/304 (53.62%), Query Frame = 0

Query: 14  INIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLRGFGDSDVLPSPSS 73
           + +H   +GSGPAV   HGFPE WYSWR+Q+  LA  G+R +A D++G+G+S   P    
Sbjct: 248 VRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEE 307

Query: 74  YTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKALVNLSVHYTPRNP 133
           Y    +  +++  LD LG+ Q   +GHDWG M+ WY  LF P+RV+A+ +L+  + P NP
Sbjct: 308 YCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANP 367

Query: 134 SISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMRDPS----PPIIPN 193
           ++SPL  ++     + Y   FQEPGVAEA+    + +   K      D S      +   
Sbjct: 368 NMSPLESIKANPVFD-YQLYFQEPGVAEAEL-EQNLSRTFKSLFRASDESVLSMHKVCEA 427

Query: 194 GFKTLATPE--TLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTGPWTGAQIK 253
           G   + +PE  +L   +TEE+I ++  +F K+GF G  N+YR  + +W+      G +I 
Sbjct: 428 GGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKIL 487

Query: 254 VPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEKAYDISSLI 312
           +P   +  + D V  +P   +++     +  +P L+    IE   H+   +K  +++ ++
Sbjct: 488 IPALMVTAEKDFVL-VPQMSQHM-----EDWIPHLKR-GHIEDCGHWTQMDKPTEVNQIL 542

BLAST of Bhi11G001019 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.7e-46
Identity = 91/256 (35.55%), Postives = 144/256 (56.25%), Query Frame = 0

Query: 13  GINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLRGFGDSDVLPSPS 72
           G+ +H   +GSGPAV   HGFPE W+SWR+Q+  LA  GFR +A D++G+G+S   P   
Sbjct: 247 GVRLHFVEMGSGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPEIE 306

Query: 73  SYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKALVNLSVHYTPRN 132
            Y+   +  D++  L+ LG+ Q   +GHDWG ++ W   LF P+RV+A+ +L+  + P N
Sbjct: 307 EYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMPSN 366

Query: 133 PSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMRD----PSPPIIP 192
           P++SP+  ++     + Y   FQEPGVAEA+    +     K F    D     +  +  
Sbjct: 367 PNVSPMEIIKANPVFD-YQLYFQEPGVAEAEL-EQNLDRTFKNFFRAHDETFLTTNRVRE 426

Query: 193 NGFKTLATPE--TLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTGPWTGAQI 252
            G   + TPE  +L   +TEEDI ++  +F K+GF G  N+YR  + +W+     +G +I
Sbjct: 427 LGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCKGSGRKI 486

Query: 253 KVPTKFIVGDLDMVYH 263
            +P   +  + D+V H
Sbjct: 487 LIPALMVTAENDLVLH 500

BLAST of Bhi11G001019 vs. NCBI nr
Match: XP_038905064.1 (epoxide hydrolase A-like [Benincasa hispida])

HSP 1 Score: 666.8 bits (1719), Expect = 9.4e-188
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0

Query: 1   MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120
           GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA
Sbjct: 61  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 180
           LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR
Sbjct: 121 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 180

Query: 181 DPSPPIIPNGFKTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTGP 240
           DPSPPIIPNGFKTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTGP
Sbjct: 181 DPSPPIIPNGFKTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTGP 240

Query: 241 WTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEKA 300
           WTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEKA
Sbjct: 241 WTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEKA 300

Query: 301 YDISSLIYDFIKKF 315
           YDISSLIYDFIKKF
Sbjct: 301 YDISSLIYDFIKKF 314

BLAST of Bhi11G001019 vs. NCBI nr
Match: XP_022984035.1 (uncharacterized protein LOC111482470 isoform X1 [Cucurbita maxima])

HSP 1 Score: 594.3 bits (1531), Expect = 5.9e-166
Identity = 275/314 (87.58%), Postives = 295/314 (93.95%), Query Frame = 0

Query: 1   MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           M +I+HSTI TNGIN+HVA IGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 345 MEQIEHSTIATNGINMHVAFIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 404

Query: 61  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120
           GFGDSDV PSPSSYTP HI+GDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA
Sbjct: 405 GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 464

Query: 121 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 180
           LVNLSVHYTPRNP+ISPL+  R  LGD+FY CKFQ+PGVAEADFGSVDTATMMKKFLT+R
Sbjct: 465 LVNLSVHYTPRNPAISPLSRTRQFLGDDFYICKFQKPGVAEADFGSVDTATMMKKFLTIR 524

Query: 181 DPSPPIIPNGFKTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTGP 240
           DPSPPIIPNGFKTL TPE+LPPWLTEEDIDYFASKF KTGFTGGFNYYRA + +WELTGP
Sbjct: 525 DPSPPIIPNGFKTLKTPESLPPWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTGP 584

Query: 241 WTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEKA 300
           W+GA+IKVPTKF+VGD+DMVYHLPGAK+YIHGGGFKK VPLLEEVVV+EGAAHFINQEKA
Sbjct: 585 WSGAKIKVPTKFVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEKA 644

Query: 301 YDISSLIYDFIKKF 315
            +IS+ IYDFI KF
Sbjct: 645 DEISAHIYDFINKF 658

BLAST of Bhi11G001019 vs. NCBI nr
Match: XP_022984036.1 (uncharacterized protein LOC111482470 isoform X2 [Cucurbita maxima] >XP_022984037.1 uncharacterized protein LOC111482470 isoform X2 [Cucurbita maxima])

HSP 1 Score: 594.3 bits (1531), Expect = 5.9e-166
Identity = 275/314 (87.58%), Postives = 295/314 (93.95%), Query Frame = 0

Query: 1   MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           M +I+HSTI TNGIN+HVA IGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MEQIEHSTIATNGINMHVAFIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120
           GFGDSDV PSPSSYTP HI+GDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 180
           LVNLSVHYTPRNP+ISPL+  R  LGD+FY CKFQ+PGVAEADFGSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPAISPLSRTRQFLGDDFYICKFQKPGVAEADFGSVDTATMMKKFLTIR 180

Query: 181 DPSPPIIPNGFKTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTGP 240
           DPSPPIIPNGFKTL TPE+LPPWLTEEDIDYFASKF KTGFTGGFNYYRA + +WELTGP
Sbjct: 181 DPSPPIIPNGFKTLKTPESLPPWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTGP 240

Query: 241 WTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEKA 300
           W+GA+IKVPTKF+VGD+DMVYHLPGAK+YIHGGGFKK VPLLEEVVV+EGAAHFINQEKA
Sbjct: 241 WSGAKIKVPTKFVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEKA 300

Query: 301 YDISSLIYDFIKKF 315
            +IS+ IYDFI KF
Sbjct: 301 DEISAHIYDFINKF 314

BLAST of Bhi11G001019 vs. NCBI nr
Match: KAG6581276.1 (hypothetical protein SDJN03_21278, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 590.5 bits (1521), Expect = 8.5e-165
Identity = 276/314 (87.90%), Postives = 293/314 (93.31%), Query Frame = 0

Query: 1   MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           M +I+HSTI TNGIN+HVASIGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MEEIEHSTIATNGINMHVASIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120
           GFGDSDV PSPSSYTP HI+GDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 180
           LVNLSVHYTPRNP+IS L+ +R LLGD+FY CKFQ+PGVAEADFGSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPAISTLSVIRKLLGDDFYICKFQKPGVAEADFGSVDTATMMKKFLTIR 180

Query: 181 DPSPPIIPNGFKTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTGP 240
           DP PPIIPNGFKTL TPE+LPPWLTEEDIDYFASKF KTGFTGG NYYRA DL+WELTGP
Sbjct: 181 DPRPPIIPNGFKTLKTPESLPPWLTEEDIDYFASKFEKTGFTGGLNYYRAFDLTWELTGP 240

Query: 241 WTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEKA 300
           WTGAQIKVP KF+VGDLDMVY+ PGAK+YIHGG FKK VPLLEEVVVIEGAAHFINQEKA
Sbjct: 241 WTGAQIKVPAKFVVGDLDMVYNSPGAKQYIHGGRFKKDVPLLEEVVVIEGAAHFINQEKA 300

Query: 301 YDISSLIYDFIKKF 315
            +IS+ IYDFI KF
Sbjct: 301 DEISAHIYDFINKF 314

BLAST of Bhi11G001019 vs. NCBI nr
Match: XP_022934351.1 (uncharacterized protein LOC111441542 [Cucurbita moschata])

HSP 1 Score: 590.1 bits (1520), Expect = 1.1e-164
Identity = 274/314 (87.26%), Postives = 293/314 (93.31%), Query Frame = 0

Query: 1   MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           M KI+HSTI TNGIN+HVASIGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MEKIEHSTIATNGINMHVASIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120
           GFGDSDV PSPSSYTP HI+GDLIGLLDHLGI+QVFLVGHDWGAMMAWYFCLFRPDRVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIEQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 180
           LVNLSVHY PRNP+ISPL+  R  LGD+FY CKFQ PGVAEADFGSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYNPRNPAISPLSRTRQFLGDDFYICKFQTPGVAEADFGSVDTATMMKKFLTIR 180

Query: 181 DPSPPIIPNGFKTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTGP 240
           DPSPPIIPNGFKTL TPETLP WLTEEDIDYFASKF KTGFTGGFNYYRA + +WELTGP
Sbjct: 181 DPSPPIIPNGFKTLKTPETLPSWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTGP 240

Query: 241 WTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEKA 300
           W+GA+IKVPTK++VGD+DMVYHLPGAK+YIHGGGFKK VPLLEEVVV+EGAAHFINQEKA
Sbjct: 241 WSGAKIKVPTKYVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEKA 300

Query: 301 YDISSLIYDFIKKF 315
            +IS+ IYDFI KF
Sbjct: 301 DEISAHIYDFIIKF 314

BLAST of Bhi11G001019 vs. ExPASy TrEMBL
Match: A0A6J1J107 (uncharacterized protein LOC111482470 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482470 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 2.9e-166
Identity = 275/314 (87.58%), Postives = 295/314 (93.95%), Query Frame = 0

Query: 1   MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           M +I+HSTI TNGIN+HVA IGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MEQIEHSTIATNGINMHVAFIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120
           GFGDSDV PSPSSYTP HI+GDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 180
           LVNLSVHYTPRNP+ISPL+  R  LGD+FY CKFQ+PGVAEADFGSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPAISPLSRTRQFLGDDFYICKFQKPGVAEADFGSVDTATMMKKFLTIR 180

Query: 181 DPSPPIIPNGFKTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTGP 240
           DPSPPIIPNGFKTL TPE+LPPWLTEEDIDYFASKF KTGFTGGFNYYRA + +WELTGP
Sbjct: 181 DPSPPIIPNGFKTLKTPESLPPWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTGP 240

Query: 241 WTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEKA 300
           W+GA+IKVPTKF+VGD+DMVYHLPGAK+YIHGGGFKK VPLLEEVVV+EGAAHFINQEKA
Sbjct: 241 WSGAKIKVPTKFVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEKA 300

Query: 301 YDISSLIYDFIKKF 315
            +IS+ IYDFI KF
Sbjct: 301 DEISAHIYDFINKF 314

BLAST of Bhi11G001019 vs. ExPASy TrEMBL
Match: A0A6J1J7K4 (uncharacterized protein LOC111482470 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482470 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 2.9e-166
Identity = 275/314 (87.58%), Postives = 295/314 (93.95%), Query Frame = 0

Query: 1   MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           M +I+HSTI TNGIN+HVA IGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 345 MEQIEHSTIATNGINMHVAFIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 404

Query: 61  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120
           GFGDSDV PSPSSYTP HI+GDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA
Sbjct: 405 GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 464

Query: 121 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 180
           LVNLSVHYTPRNP+ISPL+  R  LGD+FY CKFQ+PGVAEADFGSVDTATMMKKFLT+R
Sbjct: 465 LVNLSVHYTPRNPAISPLSRTRQFLGDDFYICKFQKPGVAEADFGSVDTATMMKKFLTIR 524

Query: 181 DPSPPIIPNGFKTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTGP 240
           DPSPPIIPNGFKTL TPE+LPPWLTEEDIDYFASKF KTGFTGGFNYYRA + +WELTGP
Sbjct: 525 DPSPPIIPNGFKTLKTPESLPPWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTGP 584

Query: 241 WTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEKA 300
           W+GA+IKVPTKF+VGD+DMVYHLPGAK+YIHGGGFKK VPLLEEVVV+EGAAHFINQEKA
Sbjct: 585 WSGAKIKVPTKFVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEKA 644

Query: 301 YDISSLIYDFIKKF 315
            +IS+ IYDFI KF
Sbjct: 645 DEISAHIYDFINKF 658

BLAST of Bhi11G001019 vs. ExPASy TrEMBL
Match: A0A6J1F1K3 (uncharacterized protein LOC111441542 OS=Cucurbita moschata OX=3662 GN=LOC111441542 PE=4 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 5.4e-165
Identity = 274/314 (87.26%), Postives = 293/314 (93.31%), Query Frame = 0

Query: 1   MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           M KI+HSTI TNGIN+HVASIGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MEKIEHSTIATNGINMHVASIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120
           GFGDSDV PSPSSYTP HI+GDLIGLLDHLGI+QVFLVGHDWGAMMAWYFCLFRPDRVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIEQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 180
           LVNLSVHY PRNP+ISPL+  R  LGD+FY CKFQ PGVAEADFGSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYNPRNPAISPLSRTRQFLGDDFYICKFQTPGVAEADFGSVDTATMMKKFLTIR 180

Query: 181 DPSPPIIPNGFKTLATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTGP 240
           DPSPPIIPNGFKTL TPETLP WLTEEDIDYFASKF KTGFTGGFNYYRA + +WELTGP
Sbjct: 181 DPSPPIIPNGFKTLKTPETLPSWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTGP 240

Query: 241 WTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEKA 300
           W+GA+IKVPTK++VGD+DMVYHLPGAK+YIHGGGFKK VPLLEEVVV+EGAAHFINQEKA
Sbjct: 241 WSGAKIKVPTKYVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEKA 300

Query: 301 YDISSLIYDFIKKF 315
            +IS+ IYDFI KF
Sbjct: 301 DEISAHIYDFIIKF 314

BLAST of Bhi11G001019 vs. ExPASy TrEMBL
Match: A0A5A7TQJ9 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G001150 PE=4 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.1e-164
Identity = 279/315 (88.57%), Postives = 290/315 (92.06%), Query Frame = 0

Query: 1   MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           M+KIQHSTI TNGINIH ASIGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MDKIQHSTISTNGINIHFASIGSGPVVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120
           GFGDSD  PSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA
Sbjct: 61  GFGDSDAPPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 180
           LVNLSVHYTPRNP+ SPLA  R  LGD+FY CKFQEPGVAEADFGSVDTATMMKKFLTMR
Sbjct: 121 LVNLSVHYTPRNPAGSPLAVTRRYLGDDFYICKFQEPGVAEADFGSVDTATMMKKFLTMR 180

Query: 181 DPSPPIIPNGFKT-LATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTG 240
           DP P IIPNGFKT L TPE LP WLTEEDI+YFASKF KTGFTGGFNYYRA D++WELTG
Sbjct: 181 DPRPAIIPNGFKTLLETPEILPSWLTEEDIEYFASKFSKTGFTGGFNYYRALDITWELTG 240

Query: 241 PWTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEK 300
           PW+ AQIKVPTKFIVGDLD+VY+ PGAKEYIHGGGFKK VPLLE+VVVIEGAAHFINQEK
Sbjct: 241 PWSRAQIKVPTKFIVGDLDLVYNFPGAKEYIHGGGFKKDVPLLEDVVVIEGAAHFINQEK 300

Query: 301 AYDISSLIYDFIKKF 315
             +ISSLIYDFI KF
Sbjct: 301 PDEISSLIYDFITKF 315

BLAST of Bhi11G001019 vs. ExPASy TrEMBL
Match: A0A1S3BZ44 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494761 PE=4 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.1e-164
Identity = 279/315 (88.57%), Postives = 290/315 (92.06%), Query Frame = 0

Query: 1   MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60
           M+KIQHSTI TNGINIH ASIGSGP VLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR
Sbjct: 1   MDKIQHSTISTNGINIHFASIGSGPVVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120
           GFGDSD  PSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA
Sbjct: 61  GFGDSDAPPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 180
           LVNLSVHYTPRNP+ SPLA  R  LGD+FY CKFQEPGVAEADFGSVDTATMMKKFLTMR
Sbjct: 121 LVNLSVHYTPRNPAGSPLAVTRRYLGDDFYICKFQEPGVAEADFGSVDTATMMKKFLTMR 180

Query: 181 DPSPPIIPNGFKT-LATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTG 240
           DP P IIPNGFKT L TPE LP WLTEEDI+YFASKF KTGFTGGFNYYRA D++WELTG
Sbjct: 181 DPRPAIIPNGFKTLLETPEILPSWLTEEDIEYFASKFSKTGFTGGFNYYRALDITWELTG 240

Query: 241 PWTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEK 300
           PW+ AQIKVPTKFIVGDLD+VY+ PGAKEYIHGGGFKK VPLLE+VVVIEGAAHFINQEK
Sbjct: 241 PWSRAQIKVPTKFIVGDLDLVYNFPGAKEYIHGGGFKKDVPLLEDVVVIEGAAHFINQEK 300

Query: 301 AYDISSLIYDFIKKF 315
             +ISSLIYDFI KF
Sbjct: 301 PDEISSLIYDFITKF 315

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G02340.12.6e-12763.61alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.12.0e-10356.66soluble epoxide hydrolase [more]
AT3G05600.11.3e-10252.63alpha/beta-Hydrolases superfamily protein [more]
AT2G26750.13.0e-9954.35alpha/beta-Hydrolases superfamily protein [more]
AT4G15960.13.1e-9649.07alpha/beta-Hydrolases superfamily protein [more]
Match NameE-valueIdentityDescription
I6YGS04.0e-5637.74Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
P349146.1e-4933.54Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
P802991.4e-4833.02Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
P349132.2e-4631.91Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
Q6Q2C23.7e-4635.55Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038905064.19.4e-188100.00epoxide hydrolase A-like [Benincasa hispida][more]
XP_022984035.15.9e-16687.58uncharacterized protein LOC111482470 isoform X1 [Cucurbita maxima][more]
XP_022984036.15.9e-16687.58uncharacterized protein LOC111482470 isoform X2 [Cucurbita maxima] >XP_022984037... [more]
KAG6581276.18.5e-16587.90hypothetical protein SDJN03_21278, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022934351.11.1e-16487.26uncharacterized protein LOC111441542 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1J1072.9e-16687.58uncharacterized protein LOC111482470 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1J7K42.9e-16687.58uncharacterized protein LOC111482470 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1F1K35.4e-16587.26uncharacterized protein LOC111441542 OS=Cucurbita moschata OX=3662 GN=LOC1114415... [more]
A0A5A7TQJ92.1e-16488.57Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BZ442.1e-16488.57bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494761 PE... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 111..124
score: 56.17
coord: 31..49
score: 56.94
coord: 248..264
score: 42.51
coord: 97..110
score: 48.7
coord: 288..310
score: 36.56
coord: 51..66
score: 56.82
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 51..66
score: 51.51
coord: 97..110
score: 31.03
coord: 111..124
score: 29.8
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 25..131
e-value: 4.4E-24
score: 85.5
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..314
e-value: 2.2E-106
score: 358.2
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 1..313
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 1..314
NoneNo IPR availablePANTHERPTHR43329:SF58OS05G0273800 PROTEINcoord: 1..314

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi11M001019Bhi11M001019mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity