Bhi08G000243 (gene) Wax gourd (B227) v1

Overview
NameBhi08G000243
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionMajor facilitator superfamily protein
Locationchr8: 10288327 .. 10290626 (+)
RNA-Seq ExpressionBhi08G000243
SyntenyBhi08G000243
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGTAGCAGCAGGGCATAGCTCCAATGGCGGAATTGGGAGCGGAGAATTGGCTGATTTTGTCCGGCAAGTGGTGGTGGGGCGGTGGTTTTCTCTTTTCGCTTCGTTCCTTGTAATGTCCGGCGCCGGCGGAGTTTATGTGTTCGCTTATTACTCTAAAGACATCAAAACGACGCTCAAATGTGATCAAACGACACTAAACAAAATTGGGTTTTATAAAGATTTGGGATCAAACGTCGGCATTGTTGCCGGACTTCTGGCGGAGGTGGCGCCCACTTGGTTAGTTCTTCTCATCGGCGCCGCCTTAAATTTTCTTGGCTACTTTAAGATATGGCAAGCCGTTACCGGAAAAATTGTCCACCCCACCGTCGGCTTCTTTTGCTTTTTCATTATGGTTGGAGCTAACTCTCAGAATTTTGCCAATACAGGTAATCCAAATTAATTAATTACTCCAAATTATTTTATTTTATTTTATTTTTATCCGTTTTGAAAATGATAGGAAAACAAATTAAATAAAAAAATTATTAATATCGAATAGGTTAATTATTTTTAAGTACAAAGTTAAAATATTAACTTTGATCACTTTTAAAAGTTCAGAAGTTAAATAAATATTAACTTAAAAGTGAGTATAGTTCCTCCATAGTTAACGTAAATTTTTCATATATCTTCATTTAATGTATTAAAAAAAGTTAAATAAAGTTCATTAGATAACTACTTGATTTTTTTATCTTTTACTTTTGGTTTTTAGTTTTTAAAATTAAATATTAATAGATAACAAAAAATAAAAATAAAAAAAACTAGTATTAGAGACTAAATTTAAGATTTATTGAATGTTCAAGGATTGAAGACTAAAATTAAACAAATTTAAAGTATAAGAATCAAAATGGTATTTATCTAATTCAATATTTTTCTTAAAACCCAACAGGTGTGCTAGTCACGTGCGTGAAGAACTTTCCAGAAAGAAGAGGGGTGATGTTGGGCCTTCTGAAGGGCTTCGTGGGCCTAAGCGGAGCCATCATGACCCAACTTTACATCGCCATTTACGGCCACGATACGAGATCTCTTGTTCTTCTAATCGCTTGGTTCCCTTCTTTAATCTCTTTGATTTTCGTCTATACGATCAGAGAAATCAAATTCGTTAAACACCCAAATGAATTCAGAGTTTTCGTTCAATTCCTTTGTCTCACAATCCTTTTATCCGTCTTCCTCACCGTCTTAATCTTCATTCAAAAACGAGTCCAATTCGATCAATCCGCTCACATCGCCATCGTCGCCGCCATTTTTGCTCTGCTTTTTGTTCCATTGTTAATCGCCATTAGAGAAGAACTCGTTCTCTGGAATTTCAACAAACGAACCCTTATTAACAACCCTTTTACTAGAATCAGAATCGAAACCCCCCAAACAACAACAACAACAACAACAACAACAAACCCCCCAATTCCTTCTTCAAATCAAACCCAAATCCAACCCAGTTCTTGTTTCGCTAATATTTTCAACAAACCCGAAAGAGGCGAAGATTACACTGTTCTTCAAGCGATTTTCAGTATTGATATGCTTATTATATGCTCCACTATGTTAATCGGCGTCGGTGCAAGCTTAACCGCCATCGACAATCTAGGACAAATCGGTGAATCTCAAGGCTACCCATCTGGAACAATCAACTTATTCATCTCTTTAGTAAGTATTTTCAATTTCACTGGTAGAATCTTTTCTGGGTTCGTCTCTGAAATCTTGCTTGAGAAATTCAAATTCCCTCGTCCGTTGATGCTTACATTCATCCTCTTGATTTCCTGTGTTGGGCATCTTTTAGTAGCTTTCCCATTTGAGGATTCGCTTTACATTGCATCAATCATTATCGGGTTTTCAATGGGTTCACAAGTTCCATTGCATTTCGCGATGATTTCAGAAATTTTTGGGTTGAAACATTACTCGACATTGTTTAATTTTGGGCAATTGTCTTGTCCAATTGGGTCTTATATTCTGAACGTGATGGTGACTGGTAAACTGTATGATGAAGTAGCGCAATTGGGGAGTAGTCCAAATAAGTTCCATTGTCAAGGGAATCATTGTTATGAACAATCGTTTTTGATTTTGAGTGGATTGACGTTTATTGTAGCGATGATATCGTTGATTTTGGTGAAGAGGACGAGGGAGTTTTATGGAGGGGATATATACAAGAAATTCAGGGAAGATATGGAAACTTTGAAGACGGAAATGGAGTTTTACAGATTGGATGAGAAGAGGACTAGGATTGGGAATTTACTTGTTGATAAACATTCTATCAATTTCAAGAGATGA

mRNA sequence

ATGATTGTAGCAGCAGGGCATAGCTCCAATGGCGGAATTGGGAGCGGAGAATTGGCTGATTTTGTCCGGCAAGTGGTGGTGGGGCGGTGGTTTTCTCTTTTCGCTTCGTTCCTTGTAATGTCCGGCGCCGGCGGAGTTTATGTGTTCGCTTATTACTCTAAAGACATCAAAACGACGCTCAAATGTGATCAAACGACACTAAACAAAATTGGGTTTTATAAAGATTTGGGATCAAACGTCGGCATTGTTGCCGGACTTCTGGCGGAGGTGGCGCCCACTTGGTTAGTTCTTCTCATCGGCGCCGCCTTAAATTTTCTTGGCTACTTTAAGATATGGCAAGCCGTTACCGGAAAAATTGTCCACCCCACCGTCGGCTTCTTTTGCTTTTTCATTATGGTTGGAGCTAACTCTCAGAATTTTGCCAATACAGGTGTGCTAGTCACGTGCGTGAAGAACTTTCCAGAAAGAAGAGGGGTGATGTTGGGCCTTCTGAAGGGCTTCGTGGGCCTAAGCGGAGCCATCATGACCCAACTTTACATCGCCATTTACGGCCACGATACGAGATCTCTTGTTCTTCTAATCGCTTGGTTCCCTTCTTTAATCTCTTTGATTTTCGTCTATACGATCAGAGAAATCAAATTCGTTAAACACCCAAATGAATTCAGAGTTTTCGTTCAATTCCTTTGTCTCACAATCCTTTTATCCGTCTTCCTCACCGTCTTAATCTTCATTCAAAAACGAGTCCAATTCGATCAATCCGCTCACATCGCCATCGTCGCCGCCATTTTTGCTCTGCTTTTTGTTCCATTGTTAATCGCCATTAGAGAAGAACTCGTTCTCTGGAATTTCAACAAACGAACCCTTATTAACAACCCTTTTACTAGAATCAGAATCGAAACCCCCCAAACAACAACAACAACAACAACAACAACAAACCCCCCAATTCCTTCTTCAAATCAAACCCAAATCCAACCCAGTTCTTGTTTCGCTAATATTTTCAACAAACCCGAAAGAGGCGAAGATTACACTGTTCTTCAAGCGATTTTCAGTATTGATATGCTTATTATATGCTCCACTATGTTAATCGGCGTCGGTGCAAGCTTAACCGCCATCGACAATCTAGGACAAATCGGTGAATCTCAAGGCTACCCATCTGGAACAATCAACTTATTCATCTCTTTAGTAAGTATTTTCAATTTCACTGGTAGAATCTTTTCTGGGTTCGTCTCTGAAATCTTGCTTGAGAAATTCAAATTCCCTCGTCCGTTGATGCTTACATTCATCCTCTTGATTTCCTGTGTTGGGCATCTTTTAGTAGCTTTCCCATTTGAGGATTCGCTTTACATTGCATCAATCATTATCGGGTTTTCAATGGGTTCACAAGTTCCATTGCATTTCGCGATGATTTCAGAAATTTTTGGGTTGAAACATTACTCGACATTGTTTAATTTTGGGCAATTGTCTTGTCCAATTGGGTCTTATATTCTGAACGTGATGGTGACTGGTAAACTGTATGATGAAGTAGCGCAATTGGGGAGTAGTCCAAATAAGTTCCATTGTCAAGGGAATCATTGTTATGAACAATCGTTTTTGATTTTGAGTGGATTGACGTTTATTGTAGCGATGATATCGTTGATTTTGGTGAAGAGGACGAGGGAGTTTTATGGAGGGGATATATACAAGAAATTCAGGGAAGATATGGAAACTTTGAAGACGGAAATGGAGTTTTACAGATTGGATGAGAAGAGGACTAGGATTGGGAATTTACTTGTTGATAAACATTCTATCAATTTCAAGAGATGA

Coding sequence (CDS)

ATGATTGTAGCAGCAGGGCATAGCTCCAATGGCGGAATTGGGAGCGGAGAATTGGCTGATTTTGTCCGGCAAGTGGTGGTGGGGCGGTGGTTTTCTCTTTTCGCTTCGTTCCTTGTAATGTCCGGCGCCGGCGGAGTTTATGTGTTCGCTTATTACTCTAAAGACATCAAAACGACGCTCAAATGTGATCAAACGACACTAAACAAAATTGGGTTTTATAAAGATTTGGGATCAAACGTCGGCATTGTTGCCGGACTTCTGGCGGAGGTGGCGCCCACTTGGTTAGTTCTTCTCATCGGCGCCGCCTTAAATTTTCTTGGCTACTTTAAGATATGGCAAGCCGTTACCGGAAAAATTGTCCACCCCACCGTCGGCTTCTTTTGCTTTTTCATTATGGTTGGAGCTAACTCTCAGAATTTTGCCAATACAGGTGTGCTAGTCACGTGCGTGAAGAACTTTCCAGAAAGAAGAGGGGTGATGTTGGGCCTTCTGAAGGGCTTCGTGGGCCTAAGCGGAGCCATCATGACCCAACTTTACATCGCCATTTACGGCCACGATACGAGATCTCTTGTTCTTCTAATCGCTTGGTTCCCTTCTTTAATCTCTTTGATTTTCGTCTATACGATCAGAGAAATCAAATTCGTTAAACACCCAAATGAATTCAGAGTTTTCGTTCAATTCCTTTGTCTCACAATCCTTTTATCCGTCTTCCTCACCGTCTTAATCTTCATTCAAAAACGAGTCCAATTCGATCAATCCGCTCACATCGCCATCGTCGCCGCCATTTTTGCTCTGCTTTTTGTTCCATTGTTAATCGCCATTAGAGAAGAACTCGTTCTCTGGAATTTCAACAAACGAACCCTTATTAACAACCCTTTTACTAGAATCAGAATCGAAACCCCCCAAACAACAACAACAACAACAACAACAACAAACCCCCCAATTCCTTCTTCAAATCAAACCCAAATCCAACCCAGTTCTTGTTTCGCTAATATTTTCAACAAACCCGAAAGAGGCGAAGATTACACTGTTCTTCAAGCGATTTTCAGTATTGATATGCTTATTATATGCTCCACTATGTTAATCGGCGTCGGTGCAAGCTTAACCGCCATCGACAATCTAGGACAAATCGGTGAATCTCAAGGCTACCCATCTGGAACAATCAACTTATTCATCTCTTTAGTAAGTATTTTCAATTTCACTGGTAGAATCTTTTCTGGGTTCGTCTCTGAAATCTTGCTTGAGAAATTCAAATTCCCTCGTCCGTTGATGCTTACATTCATCCTCTTGATTTCCTGTGTTGGGCATCTTTTAGTAGCTTTCCCATTTGAGGATTCGCTTTACATTGCATCAATCATTATCGGGTTTTCAATGGGTTCACAAGTTCCATTGCATTTCGCGATGATTTCAGAAATTTTTGGGTTGAAACATTACTCGACATTGTTTAATTTTGGGCAATTGTCTTGTCCAATTGGGTCTTATATTCTGAACGTGATGGTGACTGGTAAACTGTATGATGAAGTAGCGCAATTGGGGAGTAGTCCAAATAAGTTCCATTGTCAAGGGAATCATTGTTATGAACAATCGTTTTTGATTTTGAGTGGATTGACGTTTATTGTAGCGATGATATCGTTGATTTTGGTGAAGAGGACGAGGGAGTTTTATGGAGGGGATATATACAAGAAATTCAGGGAAGATATGGAAACTTTGAAGACGGAAATGGAGTTTTACAGATTGGATGAGAAGAGGACTAGGATTGGGAATTTACTTGTTGATAAACATTCTATCAATTTCAAGAGATGA

Protein sequence

MIVAAGHSSNGGIGSGELADFVRQVVVGRWFSLFASFLVMSGAGGVYVFAYYSKDIKTTLKCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWLVLLIGAALNFLGYFKIWQAVTGKIVHPTVGFFCFFIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDTRSLVLLIAWFPSLISLIFVYTIREIKFVKHPNEFRVFVQFLCLTILLSVFLTVLIFIQKRVQFDQSAHIAIVAAIFALLFVPLLIAIREELVLWNFNKRTLINNPFTRIRIETPQTTTTTTTTTNPPIPSSNQTQIQPSSCFANIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGVGASLTAIDNLGQIGESQGYPSGTINLFISLVSIFNFTGRIFSGFVSEILLEKFKFPRPLMLTFILLISCVGHLLVAFPFEDSLYIASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNFGQLSCPIGSYILNVMVTGKLYDEVAQLGSSPNKFHCQGNHCYEQSFLILSGLTFIVAMISLILVKRTREFYGGDIYKKFREDMETLKTEMEFYRLDEKRTRIGNLLVDKHSINFKR
Homology
BLAST of Bhi08G000243 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 608.6 bits (1568), Expect = 5.4e-174
Identity = 319/565 (56.46%), Postives = 413/565 (73.10%), Query Frame = 0

Query: 13  IGSGELADFVRQVVVGRWFSLFASFLVMSGAGGVYVFAYYSKDIKTTLKCDQTTLNKIGF 72
           +G+ E   F+     GRWF +FASFL+M+ AG  Y+F  YSKDIK+TL  DQTTLN +GF
Sbjct: 1   MGNDETVRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGF 60

Query: 73  YKDLGSNVGIVAGLLAEVAPTWLVLLIGAALNFLGYFKIWQAVTGKIVHPTVGFFCFFIM 132
           +KDLG+NVG+++GL+AEV PTW VL IG+A+NF+GYF IW  VTGK+  P V   C +I 
Sbjct: 61  FKDLGANVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYIC 120

Query: 133 VGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDTRSLVL 192
           +GANSQNFANTG LVTCVKNFPE RGVMLGLLKG+VGLSGAI TQLY AIYGHD++SL+L
Sbjct: 121 IGANSQNFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLIL 180

Query: 193 LIAWFPSLISLIFVYTIREIKFVKHPNEFRVFVQFLCLTILLSVFLTVLIFIQKRVQFDQ 252
           LIAW P+ +SL+FVY IRE K V+  NE  VF QFL ++I L++FL  +   +K+V F +
Sbjct: 181 LIAWLPAAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSK 240

Query: 253 SAHIAIVAAIFALLFVPLLIAIREELVLWNFNKRTLINNPFTRIRIETPQTTTTTTTTTN 312
           +A+ A      ALLFVPL +++++EL +WN  K   I  P + +++E P+          
Sbjct: 241 AAYAASATICCALLFVPLTVSVKQELEVWNMMKLP-IEEP-SEVKVEKPKKELDLDQDKA 300

Query: 313 PPIPSSNQTQIQPSSCFANIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGVGASLTAID 372
             +   N  + +  SCF+ +F+ P RGEDYT+LQA+ S DM+I+      G+G+SLTA+D
Sbjct: 301 AKV---NGEEKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVD 360

Query: 373 NLGQIGESQGYPSGTINLFISLVSIFNFTGRIFSGFVSEILLEKFKFPRPLMLTFILLIS 432
           NLGQIGES GYP+ T++ F+SLVSI+N+ GR+FSGFVSE LL K+K PRPLM+T +LL+S
Sbjct: 361 NLGQIGESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLS 420

Query: 433 CVGHLLVAFPFEDSLYIASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNFGQLSCPIG 492
           C GHLL+AFP   S+YIASI++GFS G+Q+PL FA+ISE+FGLK+YSTLFN GQL+ P+G
Sbjct: 421 CAGHLLIAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLG 480

Query: 493 SYILNVMVTGKLYDEVA--QLGS------SPNKFHCQGNHCYEQSFLILSGLTFIVAMIS 552
           SYILNV VTG LYD+ A  QL +            C G+ CY+  FLIL+ +TF  A++S
Sbjct: 481 SYILNVRVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVS 540

Query: 553 LILVKRTREFYGGDIYKKFREDMET 570
           L L  RTREFY GDIYKKFRE  E+
Sbjct: 541 LGLAIRTREFYKGDIYKKFRESPES 560

BLAST of Bhi08G000243 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 553.9 bits (1426), Expect = 1.6e-157
Identity = 295/569 (51.85%), Postives = 389/569 (68.37%), Query Frame = 0

Query: 16  GELADFVRQVVVGRWFSLFASFLVMSGAGGVYVFAYYSKDIKTTLKCDQTTLNKIGFYKD 75
           G +     Q++ GRWF  F S L+MS AG  Y+F  YS DIK TL  DQTTLN + F+KD
Sbjct: 8   GSMKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKD 67

Query: 76  LGSNVGIVAGLLAEVAPTWLVLLIGAALNFLGYFKIWQAVTGKIVHPTVGFFCFFIMVGA 135
           LG+NVG++AGLL EV P W +LLIGA LNF GYF IW AVT +I  P V   C +I VGA
Sbjct: 68  LGANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGA 127

Query: 136 NSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDTRSLVLLIA 195
           NSQ+FANTG LVTCVKNFPE RGV+LG+LKG+VGLSGAI+TQLY A YG DT+ L+L+I 
Sbjct: 128 NSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIG 187

Query: 196 WFPSLISLIFVYTIREIKFVKHPNEFRVFVQFLCLTILLSVFLTVLIFIQKRVQFDQSAH 255
           W P+++S  F+ TIR +K  +  NE +VF  FL +++ L+ FL V+I I K   F QS  
Sbjct: 188 WLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEF 247

Query: 256 IAIVAAIFALLFVPLLIAIREELVLWNFNKRTLINNPF-TRIRIETPQTTTTTTTTTNPP 315
               A +  LL +P+++ I EE  LW   K+  +N+P    +  E P+  ++     +  
Sbjct: 248 GGSAAVVIVLLLLPIIVVILEEKKLWK-EKQVALNDPAPINVVTEKPKLDSSEFKDDDGE 307

Query: 316 IPSSNQTQIQPSSCFANIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGVGASLTAIDNL 375
                  +++  SC+  +FN PERG+DYT+LQA+FS+DMLI+    + GVG +LTAIDNL
Sbjct: 308 ESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNL 367

Query: 376 GQIGESQGYPSGTINLFISLVSIFNFTGRIFSGFVSEILLEKFKFPRPLMLTFILLISCV 435
           GQIG S GYP  +++ F+SLVSI+N+ GR+ SG VSEI L K+KFPRPLMLT +LL+SC 
Sbjct: 368 GQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCA 427

Query: 436 GHLLVAFPFEDSLYIASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNFGQLSCPIGSY 495
           GHLL+AF     LY+AS+IIGF  G+Q PL FA+ISEIFGLK+YSTL+NFG ++ PIGSY
Sbjct: 428 GHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSY 487

Query: 496 ILNVMVTGKLYD-----EVAQLGSS---PNKFHCQGNHCYEQSFLILSGLTFIVAMISLI 555
           +LNV V G LYD     +   LG +       +C G  C++ SF+I++ +T    ++S++
Sbjct: 488 LLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMV 547

Query: 556 LVKRTREFYGGDIYKKFREDMETLKTEME 576
           LV RT++FY  DIYKKFRE  + L  EME
Sbjct: 548 LVIRTKKFYKSDIYKKFRE--KALAAEME 573

BLAST of Bhi08G000243 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 293.9 bits (751), Expect = 2.9e-79
Identity = 184/557 (33.03%), Postives = 299/557 (53.68%), Query Frame = 0

Query: 29  RWFSLFASFLVMSGAGGVYVFAYYSKDIKTTLKCDQTTLNKIGFYKDLGSNVGIVAGLLA 88
           RW    A+  + S AG  Y+F   S  IK++L  +Q  L+++G  KDLG +VG +AG L+
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLS 75

Query: 89  EVAPTWLVLLIGAALNFLGYFKIWQAVTGKIVHPTVGFFCFFIMVGANSQNFANTGVLVT 148
           E+ P W  LL+GA  N +GY  +W  VTG+     +   C  I VG N + + NTG LV+
Sbjct: 76  EILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVS 135

Query: 149 CVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDTRSLVLLIAWFPSLISLIFVYT 208
            V+NFP+ RG ++G+LKGF GL GAI++Q+Y  I+  +  SL+L++A  P+++ +  ++ 
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVCLMFF 195

Query: 209 IREIKFVK--HPNEFRVFVQFLCLTILLSVFLTVLIFIQKRVQFDQSAHIAIVAAIFALL 268
           IR +   K   P +   F     + +LL+ +L  ++ IQ  V    +        +F +L
Sbjct: 196 IRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLFVIL 255

Query: 269 FVPLLIAIREELVLWNFNKRTLINNPFTRIR------IETPQTTTTTTTTTNPP----IP 328
            VP+L+ I              I  P    R      ++TP    +      P     +P
Sbjct: 256 VVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVDLLP 315

Query: 329 SSN--------QTQIQPSSC----FANIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGV 388
           +S         Q Q+  ++       N    P RGED+T+ QA+   D  +I  ++L+G 
Sbjct: 316 ASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSLLLGS 375

Query: 389 GASLTAIDNLGQIGESQGYPSGTINLFISLVSIFNFTGRIFSGFVSEILLEKFKFPRPLM 448
           G+ LT IDNLGQ+ +S GY +   ++ +S++SI+NF GRI  G+ SE+++  + +PRP+ 
Sbjct: 376 GSGLTVIDNLGQMSQSLGYDN--THVLVSMISIWNFLGRIGGGYFSELVVRDYAYPRPVA 435

Query: 449 LTFILLISCVGHLLVAFPFEDSLYIASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNF 508
           +    LI  VGH+  A+ +  ++YI +++IG   G+   +  A  SE+FGLK +  L+NF
Sbjct: 436 MAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGALYNF 495

Query: 509 GQLSCPIGSYILNVMVTGKLYDEVAQLGSSPNKF------HCQGNHCYEQSFLILSGLTF 556
             L+ P GS + + M+   +YD  A+  +  + F       C G+ C+  + LI+SG   
Sbjct: 496 LTLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPDDALRCNGSICFFLTSLIMSGFCI 555

BLAST of Bhi08G000243 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 292.0 bits (746), Expect = 1.1e-78
Identity = 181/578 (31.31%), Postives = 309/578 (53.46%), Query Frame = 0

Query: 22  VRQVVVGRWFSLFASFLVMSGAGGVYVFAYYSKDIKTTLKCDQTTLNKIGFYKDLGSNVG 81
           V+  +  RW    A+  + S AG  Y+F   S  IK++L  +Q  L+++G  KDLG +VG
Sbjct: 9   VKSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVG 68

Query: 82  IVAGLLAEVAPTWLVLLIGAALNFLGYFKIWQAVTGKIVHPTVGFFCFFIMVGANSQNFA 141
            +AG L+E+ P W  LL+G+  N +GY  +W  VTG+     +   C  I VG N + + 
Sbjct: 69  FLAGTLSEILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYF 128

Query: 142 NTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDTRSLVLLIAWFPSLI 201
           NT  LV+ V+NFP+ RG ++G+LKGF GL GAI++Q+Y  I+  D  SL+ ++A  PS++
Sbjct: 129 NTAALVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPSVV 188

Query: 202 SLIFVYTIREIKFVK--HPNEFRVFVQFLCLTILLSVFLTVLIFIQKRVQFDQSAHIAIV 261
            +  ++ IR +   +    ++   F     + ILL+ +L  ++ ++  +    S  IA  
Sbjct: 189 VVPLMFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIAFT 248

Query: 262 AAIFALLFVPLLIAIREELVLWNFNKRTLINNP-----------------------FTRI 321
             +FA+L VP+ I I       + +    +  P                       F+ +
Sbjct: 249 VVLFAILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFSEV 308

Query: 322 RIETPQTTTTTTTTTNPPIPSSNQTQIQPSSCFANIFNK----PERGEDYTVLQAIFSID 381
             E P+              +  Q ++  ++    +  K    P RGED+T+ QA+   D
Sbjct: 309 EDEKPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKAD 368

Query: 382 MLIICSTMLIGVGASLTAIDNLGQIGESQGYPSGTINLFISLVSIFNFTGRIFSGFVSEI 441
             +I  ++L+G G+ LT IDNLGQ+ +S GY +   ++F+S++SI+NF GRI  G+ SE+
Sbjct: 369 FWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDN--THVFVSMISIWNFLGRIGGGYFSEL 428

Query: 442 LLEKFKFPRPLMLTFILLISCVGHLLVAFPFEDSLYIASIIIGFSMGSQVPLHFAMISEI 501
           ++  + +PRP+ +    L+  VGH+  A+ +  +++I +++IG   G+   +  A  SE+
Sbjct: 429 IVRDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASEL 488

Query: 502 FGLKHYSTLFNFGQLSCPIGSYILNVMVTGKLYDEVAQLGSSPNKFH------CQGNHCY 561
           FGLK +  L+NF  L+ P GS + + ++   +YD  A+  +  + F+      C+G+ CY
Sbjct: 489 FGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFNPDDVLRCRGSICY 548

Query: 562 EQSFLILSGLTFIVAMISLILVKRTREFYGGDIYKKFR 565
             + LI+SG   I A +S+ILV+RT+  Y  ++Y K R
Sbjct: 549 FLTSLIMSGFCLIAAALSMILVQRTKPVY-TNLYGKTR 583

BLAST of Bhi08G000243 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 288.5 bits (737), Expect = 1.2e-77
Identity = 180/555 (32.43%), Postives = 304/555 (54.77%), Query Frame = 0

Query: 24  QVVVGRWFSLFASFLVMSGAGGVYVFAYYSKDIKTTLKCDQTTLNKIGFYKDLGSNVGIV 83
           +++  +W ++ AS  +   +G  Y F  YS  +K+T   DQ+TL+ +  +KD+G+N G+ 
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 84  AGLLAEVAPT----------------WLVLLIGAALNFLGYFKIWQAVTGKIVHPTVGFF 143
           +GLL   A +                W+VL +GA   F GYF IW +VTG I  P V   
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 144 CFFIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDT 203
           C F+ + A SQ F NT  +V+ V+NF +  G  +G++KGF+GLSGAI+ QLY  +   D 
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDP 181

Query: 204 RSLVLLIAWFPSLISLIFVYTIREIKFVKHPNEFRVFVQFLCLTILLSVFLTVLIFIQKR 263
            S +LL+A  P+++SL+ +  +R I      ++ +       ++++++ +L ++I ++  
Sbjct: 182 ASFILLLAVTPTVLSLLVMPLVR-IYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNT 241

Query: 264 VQFDQSAHIAIVAAIFALLFVPLLIAIREELVLWNFNKRTLINNPFTRIRIETPQTTTTT 323
                 A+I  +  +  +L +PLLIA R +           + + ++ + I +P+ TT  
Sbjct: 242 FGLSSWANIVTLVCLLVMLALPLLIARRAQ----RDGMEKTVPHDYSPL-ISSPKATT-- 301

Query: 324 TTTTNPPIPSSNQTQIQPSSCFANIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGVGAS 383
                    S NQ+    S   A +       E+  +LQA+  +   ++   M+ G+G+ 
Sbjct: 302 ---------SGNQSSEGDSKVEAGL------SENLNLLQAMKKLSFWLLFLAMICGMGSG 361

Query: 384 LTAIDNLGQIGESQGYPSGTINLFISLVSIFNFTGRIFSGFVSEILLEKFKFPRPLMLTF 443
           L+ I+N+ QIGES  Y S  IN  +SL SI+NF GR  +G+ S+ LL K  +PRPL++  
Sbjct: 362 LSTINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAA 421

Query: 444 ILLISCVGHLLVAFPFEDSLYIASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNFGQL 503
            L    +GHL++A  F+ +LY+ S+I+G   GSQ  L   + SE+FG++H  T+FN   +
Sbjct: 422 TLGTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISV 481

Query: 504 SCPIGSYILNVMVTGKLYDEVAQLGSSPNKFHCQGNHCYEQSFLILSGLTFIVAMISLIL 563
           + PIGSYI +V + G +YD+ A    S     C G+HC+  SF+I++ + F   +++++L
Sbjct: 482 ASPIGSYIFSVRLIGYIYDKTA----SGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVL 529

BLAST of Bhi08G000243 vs. ExPASy TrEMBL
Match: A0A1S3CMG9 (uncharacterized protein LOC103502605 OS=Cucumis melo OX=3656 GN=LOC103502605 PE=4 SV=1)

HSP 1 Score: 1012.3 bits (2616), Expect = 8.4e-292
Identity = 516/600 (86.00%), Postives = 560/600 (93.33%), Query Frame = 0

Query: 1   MIVAAGHSSNGGIGSGELADFVRQVVVGRWFSLFASFLVMSGAGGVYVFAYYSKDIKTTL 60
           MIVAAG  SNGGI SGELADF+ QVVVGRWFSLFASFLVM+GAGGVY+FAYYS+DIKTTL
Sbjct: 1   MIVAAGQRSNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTL 60

Query: 61  KCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWLVLLIGAALNFLGYFKIWQAVTGKIV 120
           +CDQTTLN+IGFYKDLGSNVG++AGLLAEVAPTWLVLLIGAA NF+GYFKIWQAVTGKIV
Sbjct: 61  QCDQTTLNRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIV 120

Query: 121 HPTVGFFCFFIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180
            PTV FFCF+IM+GANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI
Sbjct: 121 RPTVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180

Query: 181 AIYGHDTRSLVLLIAWFPSLISLIFVYTIREIKFVKHPNEFRVFVQFLCLTILLSVFLTV 240
           AIYGHDT+SL+LLIAWFPS++SLIFVYTIREIKFVKHPNEFRVFVQFLC+T+LL++ LT 
Sbjct: 181 AIYGHDTKSLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTA 240

Query: 241 LIFIQKRVQFDQSAHIAIVAAIFALLFVPLLIAIREELVLWNFNKRTLINNPFTRIRIET 300
           LIF+QKRV FDQSAHIAIVAAI ALLFVPLLIAIREE+V+WN NKRT I+NPF RI IE+
Sbjct: 241 LIFVQKRVHFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIES 300

Query: 301 PQTTTTTTTTTNPPIPSSNQTQIQPSSCFANIFNKPERGEDYTVLQAIFSIDMLIICSTM 360
            Q      + + P  P     Q QP+SCF+NIFNKPERGEDYTVLQAIFSIDMLIICSTM
Sbjct: 301 SQPKPPIHSPSTPQHP----LQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTM 360

Query: 361 LIGVGASLTAIDNLGQIGESQGYPSGTINLFISLVSIFNFTGRIFSGFVSEILLEKFKFP 420
           LIGVGASLTAIDNLGQIGE+QGYPS TINLF+SL+SI NFTGRIFSGFVSEILLEKF+FP
Sbjct: 361 LIGVGASLTAIDNLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFP 420

Query: 421 RPLMLTFILLISCVGHLLVAFPFEDSLYIASIIIGFSMGSQVPLHFAMISEIFGLKHYST 480
           RPLMLT ILLIS +GHLLVAFPF+DSLY+ASIIIGFSMGSQVPLHFAMISEIFGLKHYST
Sbjct: 421 RPLMLTLILLISSLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYST 480

Query: 481 LFNFGQLSCPIGSYILNVMVTGKLYDEVAQLGSSPNKFHCQGNHCYEQSFLILSGLTFIV 540
           LFNFGQLSCPIGSYILNVMVTGKLYDEVA++GS+PNK HC G HCYEQSFLIL+GLTFIV
Sbjct: 481 LFNFGQLSCPIGSYILNVMVTGKLYDEVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIV 540

Query: 541 AMISLILVKRTREFYGGDIYKKFREDMETLKTEMEFYRLDEKRTRIGNLLVDKHSINFKR 600
           AMISLILV+RTREFY GDIYKKF+EDMETLKTE+EFYR+DEKRTRIGNLLVDKHSINFK+
Sbjct: 541 AMISLILVQRTREFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFKK 596

BLAST of Bhi08G000243 vs. ExPASy TrEMBL
Match: A0A5D3BP48 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2042G00080 PE=4 SV=1)

HSP 1 Score: 1007.3 bits (2603), Expect = 2.7e-290
Identity = 513/598 (85.79%), Postives = 558/598 (93.31%), Query Frame = 0

Query: 3   VAAGHSSNGGIGSGELADFVRQVVVGRWFSLFASFLVMSGAGGVYVFAYYSKDIKTTLKC 62
           +AAG  SNGGI SGELADF+ QVVVGRWFSLFASFLVM+GAGGVY+FAYYS+DIKTTL+C
Sbjct: 28  LAAGQRSNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTLQC 87

Query: 63  DQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWLVLLIGAALNFLGYFKIWQAVTGKIVHP 122
           DQTTLN+IGFYKDLGSNVG++AGLLAEVAPTWLVLLIGAA NF+GYFKIWQAVTGKIV P
Sbjct: 88  DQTTLNRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIVRP 147

Query: 123 TVGFFCFFIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAI 182
           TV FFCF+IM+GANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAI
Sbjct: 148 TVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAI 207

Query: 183 YGHDTRSLVLLIAWFPSLISLIFVYTIREIKFVKHPNEFRVFVQFLCLTILLSVFLTVLI 242
           YGHDT+SL+LLIAWFPS++SLIFVYTIREIKFVKHPNEFRVFVQFLC+T+LL++ LT LI
Sbjct: 208 YGHDTKSLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTALI 267

Query: 243 FIQKRVQFDQSAHIAIVAAIFALLFVPLLIAIREELVLWNFNKRTLINNPFTRIRIETPQ 302
           F+QKRV FDQSAHIAIVAAI ALLFVPLLIAIREE+V+WN NKRT I+NPF RI IE+ Q
Sbjct: 268 FVQKRVHFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIESSQ 327

Query: 303 TTTTTTTTTNPPIPSSNQTQIQPSSCFANIFNKPERGEDYTVLQAIFSIDMLIICSTMLI 362
                 + + P  P     Q QP+SCF+NIFNKPERGEDYTVLQAIFSIDMLIICSTMLI
Sbjct: 328 PKPPIHSPSTPQHP----LQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLI 387

Query: 363 GVGASLTAIDNLGQIGESQGYPSGTINLFISLVSIFNFTGRIFSGFVSEILLEKFKFPRP 422
           GVGASLTAIDNLGQIGE+QGYPS TINLF+SL+SI NFTGRIFSGFVSEILLEKF+FPRP
Sbjct: 388 GVGASLTAIDNLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRP 447

Query: 423 LMLTFILLISCVGHLLVAFPFEDSLYIASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLF 482
           LMLT ILLIS +GHLLVAFPF+DSLY+ASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLF
Sbjct: 448 LMLTLILLISSLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLF 507

Query: 483 NFGQLSCPIGSYILNVMVTGKLYDEVAQLGSSPNKFHCQGNHCYEQSFLILSGLTFIVAM 542
           NFGQLSCPIGSYILNVMVTGKLYDEVA++GS+PNK HC G HCYEQSFLIL+GLTFIVAM
Sbjct: 508 NFGQLSCPIGSYILNVMVTGKLYDEVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAM 567

Query: 543 ISLILVKRTREFYGGDIYKKFREDMETLKTEMEFYRLDEKRTRIGNLLVDKHSINFKR 601
           ISLILV+RTREFY GDIYKKF+EDMETLKTE+EFYR+DEKRTRIGNLLVDKHSINFK+
Sbjct: 568 ISLILVQRTREFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFKK 621

BLAST of Bhi08G000243 vs. ExPASy TrEMBL
Match: A0A6J1GAT5 (uncharacterized protein LOC111452462 OS=Cucurbita moschata OX=3662 GN=LOC111452462 PE=4 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 7.9e-258
Identity = 459/601 (76.37%), Postives = 534/601 (88.85%), Query Frame = 0

Query: 1   MIVAAGHSS-NGGIGSGELADFVRQVVVGRWFSLFASFLVMSGAGGVYVFAYYSKDIKTT 60
           MIV+   SS NGGI   EL+ F++QVV+GRWFSLFASFL+MSGAGGVY+FA YSKDIK+T
Sbjct: 1   MIVSPPPSSINGGICRAELSCFIQQVVMGRWFSLFASFLIMSGAGGVYLFASYSKDIKST 60

Query: 61  LKCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWLVLLIGAALNFLGYFKIWQAVTGKI 120
           LKCDQTTLNKIGFYKDLGSNVG++AGLLAEVAP W V+LIGAALNF+GYFKIWQAVTGKI
Sbjct: 61  LKCDQTTLNKIGFYKDLGSNVGVIAGLLAEVAPPWFVILIGAALNFVGYFKIWQAVTGKI 120

Query: 121 VHPTVGFFCFFIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLY 180
           + PTV FFCF+IMVGANSQNFANT VLV+CVKNFPERRGVMLGLLKGFVGLSGAIMTQ+Y
Sbjct: 121 IRPTVEFFCFYIMVGANSQNFANTVVLVSCVKNFPERRGVMLGLLKGFVGLSGAIMTQIY 180

Query: 181 IAIYGHDTRSLVLLIAWFPSLISLIFVYTIREIKFVKHPNEFRVFVQFLCLTILLSVFLT 240
            AIY HDT+SL+LL+AWFPSLIS++FV TIRE++ VKHPNEFRVFVQFLC+T++L++FLT
Sbjct: 181 GAIYDHDTKSLILLLAWFPSLISIVFVKTIREMRAVKHPNEFRVFVQFLCVTVILALFLT 240

Query: 241 VLIFIQKRVQFDQSAHIAIVAAIFALLFVPLLIAIREELVLWNFNKRTLINNPFTRIRIE 300
            LIF+QK+V F+Q+AHI IVAAIFALL VPLLIAIREE+++WN NKRT + N FTRIRI+
Sbjct: 241 ALIFVQKKVPFNQTAHITIVAAIFALLLVPLLIAIREEIIMWNLNKRT-VGNRFTRIRID 300

Query: 301 TPQTTTTTTTTTNPPIPSSNQTQIQPSSCFANIFNKPERGEDYTVLQAIFSIDMLIICST 360
            PQ+  + + + +    S  +   Q +SCFA IFNKP+RGED+T+ QAIFS+DMLI+ +T
Sbjct: 301 NPQSAPSCSASCSS--SSVLEQPKQSNSCFAAIFNKPKRGEDFTIPQAIFSVDMLILLTT 360

Query: 361 MLIGVGASLTAIDNLGQIGESQGYPSGTINLFISLVSIFNFTGRIFSGFVSEILLEKFKF 420
           MLIGVGA+LTAIDNLGQIGES  YP  TIN FISLVSIFNFTGRIFSGFVSEILLEKFKF
Sbjct: 361 MLIGVGANLTAIDNLGQIGESLNYPPETINSFISLVSIFNFTGRIFSGFVSEILLEKFKF 420

Query: 421 PRPLMLTFILLISCVGHLLVAFPFEDSLYIASIIIGFSMGSQVPLHFAMISEIFGLKHYS 480
           PRP+MLT ILL+SCVG+L+VAFPF +SL IASIIIGFS+GSQVPLHFAMISE FGLKHYS
Sbjct: 421 PRPMMLTLILLVSCVGYLIVAFPFNNSLCIASIIIGFSLGSQVPLHFAMISEFFGLKHYS 480

Query: 481 TLFNFGQLSCPIGSYILNVMVTGKLYDEVAQLGSSPNKFHCQGNHCYEQSFLILSGLTFI 540
           TLFNFGQLSCPIGSYILNVMV GKLYDE+A  GS+ +KFHC+G+ C+EQSF IL+GLTF+
Sbjct: 481 TLFNFGQLSCPIGSYILNVMVIGKLYDEMAMTGSNASKFHCEGSKCFEQSFTILAGLTFV 540

Query: 541 VAMISLILVKRTREFYGGDIYKKFREDMETLKTEMEFYRLDEKRTRIGNLLVDKHSINFK 600
           VAM+SL+LV+RT EFY GDIY+KFREDM++LKTEMEFY L+ KR +IGNL  DKH+INFK
Sbjct: 541 VAMVSLVLVRRTAEFYRGDIYQKFREDMDSLKTEMEFYPLESKRMKIGNLTFDKHAINFK 598

BLAST of Bhi08G000243 vs. ExPASy TrEMBL
Match: A0A6J1K965 (uncharacterized protein LOC111493395 OS=Cucurbita maxima OX=3661 GN=LOC111493395 PE=4 SV=1)

HSP 1 Score: 898.3 bits (2320), Expect = 1.8e-257
Identity = 460/603 (76.29%), Postives = 536/603 (88.89%), Query Frame = 0

Query: 1   MIVAAGHSS-NGGIGSGELADFVRQVVVGRWFSLFASFLVMSGAGGVYVFAYYSKDIKTT 60
           MIV+   SS NGGI   EL+ F++QVV GRWFSLFASFL+MSGAGGVY+FA YSKDIK+T
Sbjct: 1   MIVSPPPSSINGGICRAELSCFIQQVVTGRWFSLFASFLIMSGAGGVYLFASYSKDIKST 60

Query: 61  LKCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWLVLLIGAALNFLGYFKIWQAVTGKI 120
           LKCDQTTLNKIGFYKDLGSNVG++AGLLAEVAPTW V+LIGAALNF+GYFKIWQAVTGKI
Sbjct: 61  LKCDQTTLNKIGFYKDLGSNVGVIAGLLAEVAPTWFVILIGAALNFVGYFKIWQAVTGKI 120

Query: 121 VHPTVGFFCFFIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLY 180
           + PTV FFCF+IMVGANSQNFANT VLV+CVKNFPERRGVMLGLLKGFVGLSGAIMTQ+Y
Sbjct: 121 IRPTVEFFCFYIMVGANSQNFANTVVLVSCVKNFPERRGVMLGLLKGFVGLSGAIMTQIY 180

Query: 181 IAIYGHDTRSLVLLIAWFPSLISLIFVYTIREIKFVKHPNEFRVFVQFLCLTILLSVFLT 240
            A Y HDT+SL+LL+AWFPSLIS+IFV TIRE++ VKHPNEFRVFVQFLC+TI+L++FLT
Sbjct: 181 GAFYDHDTKSLILLLAWFPSLISIIFVKTIREMRAVKHPNEFRVFVQFLCVTIILALFLT 240

Query: 241 VLIFIQKRVQFDQSAHIAIVAAIFALLFVPLLIAIREELVLWNFNKRTLINNPFTRIRIE 300
            LIF+QK+VQF+Q+AHI IVAAI ALL VPLLIAIREE+++WN NKRT + N FTRIRI+
Sbjct: 241 ALIFVQKKVQFNQTAHITIVAAILALLLVPLLIAIREEIIMWNLNKRT-VGNRFTRIRID 300

Query: 301 TPQTTTTTTTTTNPPIPSSNQTQI--QPSSCFANIFNKPERGEDYTVLQAIFSIDMLIIC 360
            PQ+  + + + +    SS+  ++  Q +SCFANIFNKP+RGED+T+ QAIFS+DMLI+ 
Sbjct: 301 NPQSAPSCSASCS----SSSVLELPKQSNSCFANIFNKPKRGEDFTIPQAIFSVDMLILL 360

Query: 361 STMLIGVGASLTAIDNLGQIGESQGYPSGTINLFISLVSIFNFTGRIFSGFVSEILLEKF 420
           +TMLIGVGA+LTAIDNLGQIGES  YP  TIN FISLVSIFNFTGRIFSGFVSEILLEKF
Sbjct: 361 TTMLIGVGANLTAIDNLGQIGESLNYPPETINSFISLVSIFNFTGRIFSGFVSEILLEKF 420

Query: 421 KFPRPLMLTFILLISCVGHLLVAFPFEDSLYIASIIIGFSMGSQVPLHFAMISEIFGLKH 480
           KFPRP+MLT I L+SCVG+L+VAFPF +SL IASIIIGFS+GSQVPLHFAMISE FGLKH
Sbjct: 421 KFPRPMMLTLIHLVSCVGYLIVAFPFNNSLCIASIIIGFSLGSQVPLHFAMISEFFGLKH 480

Query: 481 YSTLFNFGQLSCPIGSYILNVMVTGKLYDEVAQLGSSPNKFHCQGNHCYEQSFLILSGLT 540
           YSTLFNFGQLSCPIGSYILNVMV GKLYDE+A  G++ ++FHC+G+ C+EQSF IL+GLT
Sbjct: 481 YSTLFNFGQLSCPIGSYILNVMVIGKLYDEMALTGNNASEFHCEGSKCFEQSFTILAGLT 540

Query: 541 FIVAMISLILVKRTREFYGGDIYKKFREDMETLKTEMEFYRLDEKRTRIGNLLVDKHSIN 600
           F+VAM+SL+LV+RT EFY GDIY+KFREDM++LKTEMEFY L+ KR +IGNL  DKH+IN
Sbjct: 541 FVVAMVSLVLVRRTAEFYRGDIYQKFREDMDSLKTEMEFYPLESKRMKIGNLTFDKHAIN 598

BLAST of Bhi08G000243 vs. ExPASy TrEMBL
Match: A0A6J1I5D1 (uncharacterized protein LOC111469394 OS=Cucurbita maxima OX=3661 GN=LOC111469394 PE=4 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 3.9e-257
Identity = 468/599 (78.13%), Postives = 516/599 (86.14%), Query Frame = 0

Query: 1   MIVAAGHSSNGGIGSGELADFVRQVVVGRWFSLFASFLVMSGAGGVYVFAYYSKDIKTTL 60
           MIV+A  SSN GIGSGEL  FVRQVV GRWFSLFASFLVM+GAGGVY+FAYYS DIKTTL
Sbjct: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL 60

Query: 61  KCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWLVLLIGAALNFLGYFKIWQAVTGKIV 120
           +CDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTW++LLIGAALNF+GYFKIWQ VTGKIV
Sbjct: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120

Query: 121 HPTVGFFCFFIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180
            PTV +FCF+IMVGANSQNFANTGVLVTCVKNFPERRGVMLGL KGFVGLSGAIMTQ+Y 
Sbjct: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180

Query: 181 AIYGHDTRSLVLLIAWFPSLISLIFVYTIREIKFVKHPNEFRVFVQFLCLTILLSVFLTV 240
           AIYG DTRSL+LL+ W PSLISLIF+ TIREIK VKHPNEFRVFV FLC++++L++FL  
Sbjct: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240

Query: 241 LIFIQKRVQFDQSAHIAIVAAIFALLFVPLLIAIREELVLWNFNKRTLINNPFTRIRIET 300
           LIFIQKR++FDQSAH A+VAAI ALL +PLLIAIREE+VLWN NKRT        I I  
Sbjct: 241 LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRT--------IGIHP 300

Query: 301 PQTTTTTTTTTNPPIPSSNQTQIQPSSCFANIFNKPERGEDYTVLQAIFSIDMLIICSTM 360
           PQ       + NPP PSS+              NKP RGED+T+ QAIFS DMLI+C TM
Sbjct: 301 PQ------ASPNPP-PSSS--------------NKPSRGEDHTIPQAIFSADMLILCITM 360

Query: 361 LIGVGASLTAIDNLGQIGESQGYPSGTINLFISLVSIFNFTGRIFSGFVSEILLEKFKFP 420
           LIGVGASLTAIDNL QIGES GYPS TIN  I LVSIFNFTGRIFSGFVS+ILLEKFKFP
Sbjct: 361 LIGVGASLTAIDNLRQIGESHGYPSETINSLIVLVSIFNFTGRIFSGFVSDILLEKFKFP 420

Query: 421 RPLMLTFILLISCVGHLLVAFPFEDSLYIASIIIGFSMGSQVPLHFAMISEIFGLKHYST 480
           RPLMLT +LL+SCVGHLLVAFPF  SLYIASI+IGFS+G+QVPLHFAMISE+FGLKHYST
Sbjct: 421 RPLMLTLVLLVSCVGHLLVAFPFHGSLYIASIVIGFSLGAQVPLHFAMISELFGLKHYST 480

Query: 481 LFNFGQLSCPIGSYILNVMVTGKLYDEVAQLGSSPNKFHCQGNHCYEQSFLILSGLTFIV 540
           LFNFGQLSCPIGSYILNVMVTG+LYDE+A+  S     HC+G+ CYEQSF+IL+GLTF V
Sbjct: 481 LFNFGQLSCPIGSYILNVMVTGRLYDEMAKATSGIGGLHCEGHSCYEQSFVILAGLTFFV 540

Query: 541 AMISLILVKRTREFYGGDIYKKFREDMETLKTEMEFYRLDEKRTRIGNLLVDKHSINFK 600
           A++SLILV RTREFY GDIYKKFREDME+LKT+MEFY LD+KRTRIGNLLVDKHSINFK
Sbjct: 541 ALVSLILVGRTREFYRGDIYKKFREDMESLKTKMEFYTLDDKRTRIGNLLVDKHSINFK 570

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G28120.15.4e-17456.46Major facilitator superfamily protein [more]
AT2G39210.11.6e-15751.85Major facilitator superfamily protein [more]
AT5G14120.12.9e-7933.03Major facilitator superfamily protein [more]
AT3G01930.21.1e-7831.31Major facilitator superfamily protein [more]
AT1G74780.11.2e-7732.43Nodulin-like / Major Facilitator Superfamily protein [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A1S3CMG98.4e-29286.00uncharacterized protein LOC103502605 OS=Cucumis melo OX=3656 GN=LOC103502605 PE=... [more]
A0A5D3BP482.7e-29085.79Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1GAT57.9e-25876.37uncharacterized protein LOC111452462 OS=Cucurbita moschata OX=3662 GN=LOC1114524... [more]
A0A6J1K9651.8e-25776.29uncharacterized protein LOC111493395 OS=Cucurbita maxima OX=3661 GN=LOC111493395... [more]
A0A6J1I5D13.9e-25778.13uncharacterized protein LOC111469394 OS=Cucurbita maxima OX=3661 GN=LOC111469394... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 560..580
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 303..322
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 14..571
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 14..571
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 342..556
e-value: 1.1E-12
score: 49.7
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 256..552
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 34..270
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 29..274
e-value: 2.8E-79
score: 266.1

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M000243Bhi08M000243mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity