Bhi08G000177 (gene) Wax gourd (B227) v1

Overview


Name	Bhi08G000177
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Carbonic anhydrase
Location	chr8: 7248875 .. 7251363 (+)
RNA-Seq Expression	Bhi08G000177
Synteny	Bhi08G000177

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGAAGCTCTTGTGCTCTTTCTTCTTTTCCATTCTTTTGCTTTCATTCAAGGCAAGATCCCAAGAAGTTGGTGAGACTCCCAACTTATGAAATGTTTTTTCTTTTTCGGTTTAAATCGTTTTAGTTTAATCTTGGTCCGGGAGATCCCAACGAGATACATGCACTACAACAAAACTGAAATTTCATGACATTTAAAAACCGTCACAATTAAAATTTGCGAGAACTATTTTCAGTTATTGTATTGACTGTCAAGGTATGAGTATCAATAACAATTATGTCATGAAAACTCACTTTTCTTGTATTGATGCGCTACTCTTATCATTACCAATTGATTTTGAGATAACATTACTTTACGTTATCTAATATTGTACCATAGTTCATGAAGCCTAAAACGAGCATTCCGTCTAAGATGGTGAACCCAAAGAAGCTCCATTTTGAGGGACATATTGGTGGATCCCACATTGAAATTAAAGGATCTTACCATCTTTAAGATCATACGTGAACTATTTCTCTTAGTATGAATTGATTTTGAGATGAAACTTCATGTTATTTAATAGTTTCACATGTTTTAACGTTTTTCAATTAGAGGAGTTAAACCACCTAAGAGAAAAAGTTTAGAATTTGGTGTAAATTTGGCTTGTAATTTTTCTTTTTCAAATTGTATTTTTATTATTATATTTTTGGATTTAAGGATTATATATGATTGTTTTATGTAGATGATGAAAGAGGGTTTGATTATGAAGGAGATGGAGAAAAGGGACCAATTCATTGGGGAGAGCTTAGAGCAGAGTGGCATACATGCAAGGTTGGGTTGATGCAATCTCCAATTGATTTATTGCATGAAAGAGTTGAAGTTATTTCTCACTTGGGAGACCTTAAAATCAACTACAAATCCTCTAATGCCACACTCAAGAATAGAGGCCATGATATGATGGTAATTAAATTCTTTTTTTAATTATAAATTACACTTTTGGTTTCTAAGTTTTGGGTTTAGTTTCTATTTGATTTCTAAATTTCTAAATATTATGCTTTTAGTATATAAATTCTGATTTTAATTTCAATTTAGTCCCTACGTTTCAAAATGTTACAATTTTACCCTTGATATTTGAGTTTTGTTTCAATTTGGTCTCTAATTTTGAAGACTTACACTCACATCAGTCTTTGGTGTTAATATCCGCTAATTAATTTAATGTAATTAAAACAAAAGGTGATTTTAATCGTTTTGAAATCACTCTCGAACATTAGATCTATAATATCTACGTCACATATATATAAAAGAAAAGTATTTGTGGTTATACAGATGAAATGGGTGGATGGTGGTGGATACATGGAAATGAATGGAACTCAATATAAACTAAAACAATGTCATTGGCACTCACCTTCCGAACACACCATTGATGGCAAGAAATTTGACCTTGAAGCTCACTTAGTCCATGAAAGCTCAAATGGGATGATTTCTGTCATTGGAATCTTGTATCAGATCGGAGAACCTGATTACTTCTTATCCACGGTGAGTTTAATGCATAATCGGGTCGATTTGTTTCGTAAACAATTTAAATTTTATTTTCTGTTTTTCGATTCATCGAGTTCGTGAATATGGATTCGATAGATGTATTTTTTAAAGTTGTTCTTAGATTTTTTGATTATTGCAGATTTTAGAACACAAGTTTTTATGTTTTTGTTGTATCCAAATGTCCAATTTGAAATTTGAAATTATAAATTTTATAAAAAGTATGAAAACTTGGATAAATATAGTGTTTTCATGTTTTAAGATATTCCATCAATTTTTGTCCCAATAAAAATATGTATAAGATATTATATTATATATTAATGGTATTATATTGTAAAATTATAATTATACAAAAAGAAGATTTAACATAAAACATGAATGTTGATAAATTGATTAATAATAATATGTATTAAACATAATAGTGGGTAATTATAATTAATTTTATTATTCACAAATATATTGTATCATATATTTTGAACCATTGTTTAAATGAGATTTATTTTTCAAATCTACTATCAACATTAATATAATAAAAATATGAAATACAACTCTATTTTCATTTGCTCTTTTAAATTTAAAATAATTATTTTAAATGATAAAATTATTAAAAATATTTTTAAATATAGTAAAATATCATTGTCTATCCGTGATAGACACTAATAGACATTTCTATAAGTATCTATCCGTGATAGATAATAACATTTTGCTATAATTATAAATAAGTTGGCTTATTTTCCTATATTTGAAAACACCTCTTAAATTTATGTTTTCAATTTAGTTCACAATAATTTCAATTTCATTGTATCAGATTAAAGGTGATTTAGAAATGGTATCAGATACCCATGAAGAAGAGAGAGTGATTGGAATAATTGATCCAAATCTATTGAAGATGGGCAGCAAACAATACTATAGATACATTGGCTCACTTACTGTTCCACCCTGCACTGAAAATGTGATGTGGACTATGATTCACCAG

mRNA sequence

ATGGGGAAGCTCTTGTGCTCTTTCTTCTTTTCCATTCTTTTGCTTTCATTCAAGGCAAGATCCCAAGAAGTTGATGATGAAAGAGGGTTTGATTATGAAGGAGATGGAGAAAAGGGACCAATTCATTGGGGAGAGCTTAGAGCAGAGTGGCATACATGCAAGGTTGGGTTGATGCAATCTCCAATTGATTTATTGCATGAAAGAGTTGAAGTTATTTCTCACTTGGGAGACCTTAAAATCAACTACAAATCCTCTAATGCCACACTCAAGAATAGAGGCCATGATATGATGGTGATTTTAATCGTTTTGAAATCACTCTCGAACATTAGATCTATAATATCTACGTCACATATATATAAAAGAAAAGTATTTGTGGTTATACAGATGAAATGGGTGGATGGTGGTGGATACATGGAAATGAATGGAACTCAATATAAACTAAAACAATGTCATTGGCACTCACCTTCCGAACACACCATTGATGGCAAGAAATTTGACCTTGAAGCTCACTTAGTCCATGAAAGCTCAAATGGGATGATTTCTGTCATTGGAATCTTGTATCAGATCGGAGAACCTGATTACTTCTTATCCACGATTAAAGGTGATTTAGAAATGGTATCAGATACCCATGAAGAAGAGAGAGTGATTGGAATAATTGATCCAAATCTATTGAAGATGGGCAGCAAACAATACTATAGATACATTGGCTCACTTACTGTTCCACCCTGCACTGAAAATGTGATGTGGACTATGATTCACCAG

Coding sequence (CDS)

ATGGGGAAGCTCTTGTGCTCTTTCTTCTTTTCCATTCTTTTGCTTTCATTCAAGGCAAGATCCCAAGAAGTTGATGATGAAAGAGGGTTTGATTATGAAGGAGATGGAGAAAAGGGACCAATTCATTGGGGAGAGCTTAGAGCAGAGTGGCATACATGCAAGGTTGGGTTGATGCAATCTCCAATTGATTTATTGCATGAAAGAGTTGAAGTTATTTCTCACTTGGGAGACCTTAAAATCAACTACAAATCCTCTAATGCCACACTCAAGAATAGAGGCCATGATATGATGGTGATTTTAATCGTTTTGAAATCACTCTCGAACATTAGATCTATAATATCTACGTCACATATATATAAAAGAAAAGTATTTGTGGTTATACAGATGAAATGGGTGGATGGTGGTGGATACATGGAAATGAATGGAACTCAATATAAACTAAAACAATGTCATTGGCACTCACCTTCCGAACACACCATTGATGGCAAGAAATTTGACCTTGAAGCTCACTTAGTCCATGAAAGCTCAAATGGGATGATTTCTGTCATTGGAATCTTGTATCAGATCGGAGAACCTGATTACTTCTTATCCACGATTAAAGGTGATTTAGAAATGGTATCAGATACCCATGAAGAAGAGAGAGTGATTGGAATAATTGATCCAAATCTATTGAAGATGGGCAGCAAACAATACTATAGATACATTGGCTCACTTACTGTTCCACCCTGCACTGAAAATGTGATGTGGACTATGATTCACCAG

Protein sequence

MGKLLCSFFFSILLLSFKARSQEVDDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLMQSPIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMVILIVLKSLSNIRSIISTSHIYKRKVFVVIQMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKGDLEMVSDTHEEERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQ

Homology

[Close]

BLAST of Bhi08G000177 vs. TAIR 10
Match: AT1G08080.1 (alpha carbonic anhydrase 7 )

HSP 1 Score: 242.3 bits (617), Expect = 4.3e-64
Identity = 116/256 (45.31%), Postives = 167/256 (65.23%), Query Frame = 0


Query: 6   CSFF------FSILLLSFKARSQ-EVDDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLM 65
           C FF      F+I+ +S  A S  EV+DER F+Y+ + EKGP  WGEL+ EW  C  G M
Sbjct: 9   CIFFVALFSIFTIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEM 68

Query: 66  QSPIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMVILIVLKSLSNIRSIISTSHI 125
           QSPIDL++ERV ++SHLG L  +Y  SNATLKNRGHD+M                     
Sbjct: 69  QSPIDLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIM--------------------- 128

Query: 126 YKRKVFVVIQMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNG 185
                     +K+ DG G +++NG +Y+L+Q HWHSPSEHTI+G++F LE H+VHE  N 
Sbjct: 129 ----------LKFEDGAGTIKINGFEYELQQLHWHSPSEHTINGRRFALELHMVHEGRNR 188

Query: 186 MISVIGILYQIGEPDYFLSTIKGDLEMVSDTHEEERVIGIIDPNLLKMGSKQYYRYIGSL 245
            ++V+ +LY+IG  D F+ +++ +LE +++  E E+ +G+IDP  +K+GS++YYRY GSL
Sbjct: 189 RMAVVTVLYKIGRADTFIRSLEKELEGIAEMEEAEKNVGMIDPTKIKIGSRKYYRYTGSL 233

Query: 246 TVPPCTENVMWTMIHQ 255
           T PPCT+NV W+++ +
Sbjct: 249 TTPPCTQNVTWSVVRK 233

[Close]

BLAST of Bhi08G000177 vs. TAIR 10
Match: AT2G28210.1 (alpha carbonic anhydrase 2 )

HSP 1 Score: 230.7 bits (587), Expect = 1.3e-60
Identity = 106/229 (46.29%), Postives = 149/229 (65.07%), Query Frame = 0


Query: 24  VDDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLMQSPIDLLHERVEVISHLGDLKINYK 83
           V+DE  F YE + E GP  WG+LR EW  C  G MQSPIDL+++RV +++HL  L  +YK
Sbjct: 5   VEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPIDLMNKRVRLVTHLKKLTRHYK 64

Query: 84  SSNATLKNRGHDMMVILIVLKSLSNIRSIISTSHIYKRKVFVVIQMKWVDGGGYMEMNGT 143
             NATLKNRGHDMM+                                  +G G + +NGT
Sbjct: 65  PCNATLKNRGHDMMLKF------------------------------GEEGSGSITVNGT 124

Query: 144 QYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKGDL 203
           +YKL Q HWHSPSEHT++G++F LE H+VHE+ NG ++V+ +LY+IG PD FL  ++  L
Sbjct: 125 EYKLLQLHWHSPSEHTMNGRRFALELHMVHENINGSLAVVTVLYKIGRPDSFLGLLENKL 184

Query: 204 EMVSDTHEEERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMI 253
             ++D +E E+ + +IDP  +K+GS+++YRYIGSLT PPCT+NV+WT++
Sbjct: 185 SAITDQNEAEKYVDVIDPRDIKIGSRKFYRYIGSLTTPPCTQNVIWTVV 203

[Close]

BLAST of Bhi08G000177 vs. TAIR 10
Match: AT1G08065.1 (alpha carbonic anhydrase 5 )

HSP 1 Score: 223.0 bits (567), Expect = 2.7e-58
Identity = 108/251 (43.03%), Postives = 154/251 (61.35%), Query Frame = 0


Query: 1   MGKLLCSFFFSILLLSFKARSQEVDDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLMQS 60
           +G +    F SI ++S      EV+DE  F+YE  GEKGP +WG L+ EW  C  G MQS
Sbjct: 6   IGYVFFLIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQS 65

Query: 61  PIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMVILIVLKSLSNIRSIISTSHIYK 120
           PIDL  +RV +  +LG L+  Y  SNAT+KNRGHD+M                       
Sbjct: 66  PIDLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIM----------------------- 125

Query: 121 RKVFVVIQMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMI 180
                 ++ +  + G  + +NGT+YKL+Q HWHSPSEHT++GK+F LE H+VH+S +G  
Sbjct: 126 ------MKFEGGNAGLGITINGTEYKLQQIHWHSPSEHTLNGKRFVLEEHMVHQSKDGRN 185

Query: 181 SVIGILYQIGEPDYFLSTIKGDLEMVSDTHEEERVIGIIDPNLLKMGSKQYYRYIGSLTV 240
           +V+   Y++G+PDYFL T++  L+ ++DTHE +  + ++ P      SK YYR+IGSLT 
Sbjct: 186 AVVAFFYKLGKPDYFLLTLERYLKRITDTHESQEFVEMVHPRTFGFESKHYYRFIGSLTT 227

Query: 241 PPCTENVMWTM 252
           PPC+ENV+WT+
Sbjct: 246 PPCSENVIWTI 227

[Close]

BLAST of Bhi08G000177 vs. TAIR 10
Match: AT4G21000.1 (alpha carbonic anhydrase 6 )

HSP 1 Score: 194.1 bits (492), Expect = 1.3e-49
Identity = 95/231 (41.13%), Postives = 138/231 (59.74%), Query Frame = 0


Query: 21  SQEVDDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLMQSPIDLLHERVEVISHLGDLKI 80
           ++E+ ++  F Y+   EKGP  WG+L  +W  C  G +QSPIDL  ERV +I H   L  
Sbjct: 28  AREIGNKPLFTYKQKTEKGPAEWGKLDPQWKVCSTGKIQSPIDLTDERVSLI-HDQALSK 87

Query: 81  NYKSSNATLKNRGHDMMVILIVLKSLSNIRSIISTSHIYKRKVFVVIQMKWVDGGGYMEM 140
           +YK ++A +++RGHD+MV                                W   GG + +
Sbjct: 88  HYKPASAVIQSRGHDVMV-------------------------------SWKGDGGKITI 147

Query: 141 NGTQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIK 200
           + T YKL QCHWHSPSEHTI+G  +DLE H+VH S++G  +V+G+LY++GEPD FL+ I 
Sbjct: 148 HQTDYKLVQCHWHSPSEHTINGTSYDLELHMVHTSASGKTTVVGVLYKLGEPDEFLTKI- 207

Query: 201 GDLEMVSDTHEEERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTM 252
             L  +    ++E  +GI+DP  ++  +  +YRYIGSLT+PPCTE V+WT+
Sbjct: 208 --LNGIKGVGKKEIDLGIVDPRDIRFETNNFYRYIGSLTIPPCTEGVIWTV 223

[Close]

BLAST of Bhi08G000177 vs. TAIR 10
Match: AT4G20990.1 (alpha carbonic anhydrase 4 )

HSP 1 Score: 193.4 bits (490), Expect = 2.3e-49
Identity = 96/230 (41.74%), Postives = 133/230 (57.83%), Query Frame = 0


Query: 23  EVDDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLMQSPIDLLHERVEVISHLGDLKINY 82
           EVDDE  F YE   EKGP  WG++   W  C  G  QSPIDL +ERV +I H       Y
Sbjct: 29  EVDDETPFTYEQKTEKGPEGWGKINPHWKVCNTGRYQSPIDLTNERVSLI-HDQAWTRQY 88

Query: 83  KSSNATLKNRGHDMMVILIVLKSLSNIRSIISTSHIYKRKVFVVIQMKWVDGGGYMEMNG 142
           K + A + NRGHD+MV                                W    G M +  
Sbjct: 89  KPAPAVITNRGHDIMV-------------------------------SWKGDAGKMTIRK 148

Query: 143 TQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKGD 202
           T + L QCHWHSPSEHT++G ++DLE H+VH S+ G  +VIG+LY++GEP+ FL+ +   
Sbjct: 149 TDFNLVQCHWHSPSEHTVNGTRYDLELHMVHTSARGRTAVIGVLYKLGEPNEFLTKLLNG 208

Query: 203 LEMVSDTHEEERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMI 253
           ++ V +   +E  +G+IDP  ++  ++++YRYIGSLTVPPCTE V+WT++
Sbjct: 209 IKAVGN---KEINLGMIDPREIRFQTRKFYRYIGSLTVPPCTEGVIWTVV 223

[Close]

BLAST of Bhi08G000177 vs. ExPASy Swiss-Prot
Match: Q8L817 (Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 6.0e-63
Identity = 116/256 (45.31%), Postives = 167/256 (65.23%), Query Frame = 0


Query: 6   CSFF------FSILLLSFKARSQ-EVDDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLM 65
           C FF      F+I+ +S  A S  EV+DER F+Y+ + EKGP  WGEL+ EW  C  G M
Sbjct: 9   CIFFVALFSIFTIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEM 68

Query: 66  QSPIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMVILIVLKSLSNIRSIISTSHI 125
           QSPIDL++ERV ++SHLG L  +Y  SNATLKNRGHD+M                     
Sbjct: 69  QSPIDLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIM--------------------- 128

Query: 126 YKRKVFVVIQMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNG 185
                     +K+ DG G +++NG +Y+L+Q HWHSPSEHTI+G++F LE H+VHE  N 
Sbjct: 129 ----------LKFEDGAGTIKINGFEYELQQLHWHSPSEHTINGRRFALELHMVHEGRNR 188

Query: 186 MISVIGILYQIGEPDYFLSTIKGDLEMVSDTHEEERVIGIIDPNLLKMGSKQYYRYIGSL 245
            ++V+ +LY+IG  D F+ +++ +LE +++  E E+ +G+IDP  +K+GS++YYRY GSL
Sbjct: 189 RMAVVTVLYKIGRADTFIRSLEKELEGIAEMEEAEKNVGMIDPTKIKIGSRKYYRYTGSL 233

Query: 246 TVPPCTENVMWTMIHQ 255
           T PPCT+NV W+++ +
Sbjct: 249 TTPPCTQNVTWSVVRK 233

[Close]

BLAST of Bhi08G000177 vs. ExPASy Swiss-Prot
Match: F4IHR4 (Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2)

HSP 1 Score: 236.9 bits (603), Expect = 2.5e-61
Identity = 112/249 (44.98%), Postives = 159/249 (63.86%), Query Frame = 0


Query: 4   LLCSFFFSILLLSFKARSQEVDDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLMQSPID 63
           +L SF  ++  LS     +EV+DE  F YE + E GP  WG+LR EW  C  G MQSPID
Sbjct: 13  VLTSFVTTVSCLSAATDYREVEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPID 72

Query: 64  LLHERVEVISHLGDLKINYKSSNATLKNRGHDMMVILIVLKSLSNIRSIISTSHIYKRKV 123
           L+++RV +++HL  L  +YK  NATLKNRGHDMM+                         
Sbjct: 73  LMNKRVRLVTHLKKLTRHYKPCNATLKNRGHDMMLKF----------------------- 132

Query: 124 FVVIQMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMISVI 183
                    +G G + +NGT+YKL Q HWHSPSEHT++G++F LE H+VHE+ NG ++V+
Sbjct: 133 -------GEEGSGSITVNGTEYKLLQLHWHSPSEHTMNGRRFALELHMVHENINGSLAVV 192

Query: 184 GILYQIGEPDYFLSTIKGDLEMVSDTHEEERVIGIIDPNLLKMGSKQYYRYIGSLTVPPC 243
            +LY+IG PD FL  ++  L  ++D +E E+ + +IDP  +K+GS+++YRYIGSLT PPC
Sbjct: 193 TVLYKIGRPDSFLGLLENKLSAITDQNEAEKYVDVIDPRDIKIGSRKFYRYIGSLTTPPC 231

Query: 244 TENVMWTMI 253
           T+NV+WT++
Sbjct: 253 TQNVIWTVV 231

[Close]

BLAST of Bhi08G000177 vs. ExPASy Swiss-Prot
Match: Q84UV8 (Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS=Nicotiana langsdorffii x Nicotiana sanderae OX=164110 GN=NEC3 PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 5.7e-61
Identity = 125/252 (49.60%), Postives = 160/252 (63.49%), Query Frame = 0


Query: 1   MGKLLCSFFFSILLLS--FKARSQEVDDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLM 60
           M  +    F S L LS  F ARS EVDDE  F Y+   E GP +WG +R +W  C  G +
Sbjct: 3   MAAITKMLFISFLFLSSVFLARSGEVDDESEFSYDEKSENGPANWGNIRPDWKECS-GKL 62

Query: 61  QSPIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMVILIVLKSLSNIRSIISTSHI 120
           QSPID+   R EV+S+L  L+ +YK SNATL NRGHD+M+ L                  
Sbjct: 63  QSPIDIFDLRAEVVSNLRILQKDYKPSNATLLNRGHDIMLRL------------------ 122

Query: 121 YKRKVFVVIQMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNG 180
                         D GGY+++N TQY+LKQ HWH+PSEHTI+G++F+LEAHLVHES+NG
Sbjct: 123 --------------DDGGYLKINETQYQLKQLHWHTPSEHTINGERFNLEAHLVHESNNG 182

Query: 181 MISVIGILYQIG-EPDYFLSTIKGDLEMVSDTHEEERVIGIIDPNLLKMGSKQYYRYIGS 240
              VIGI+Y+IG  PD FLS I+ DL++ ++    ER IGIIDPN +K+  K+Y+RYIGS
Sbjct: 183 KFVVIGIVYEIGLWPDPFLSMIENDLKVPANKKGIERGIGIIDPNQIKLDGKKYFRYIGS 221

Query: 241 LTVPPCTENVMW 250
           LT PPCTE V+W
Sbjct: 243 LTTPPCTEGVVW 221

[Close]

BLAST of Bhi08G000177 vs. ExPASy Swiss-Prot
Match: F4HUC4 (Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 3.8e-57
Identity = 108/251 (43.03%), Postives = 154/251 (61.35%), Query Frame = 0


Query: 1   MGKLLCSFFFSILLLSFKARSQEVDDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLMQS 60
           +G +    F SI ++S      EV+DE  F+YE  GEKGP +WG L+ EW  C  G MQS
Sbjct: 6   IGYVFFLIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQS 65

Query: 61  PIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMVILIVLKSLSNIRSIISTSHIYK 120
           PIDL  +RV +  +LG L+  Y  SNAT+KNRGHD+M                       
Sbjct: 66  PIDLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIM----------------------- 125

Query: 121 RKVFVVIQMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMI 180
                 ++ +  + G  + +NGT+YKL+Q HWHSPSEHT++GK+F LE H+VH+S +G  
Sbjct: 126 ------MKFEGGNAGLGITINGTEYKLQQIHWHSPSEHTLNGKRFVLEEHMVHQSKDGRN 185

Query: 181 SVIGILYQIGEPDYFLSTIKGDLEMVSDTHEEERVIGIIDPNLLKMGSKQYYRYIGSLTV 240
           +V+   Y++G+PDYFL T++  L+ ++DTHE +  + ++ P      SK YYR+IGSLT 
Sbjct: 186 AVVAFFYKLGKPDYFLLTLERYLKRITDTHESQEFVEMVHPRTFGFESKHYYRFIGSLTT 227

Query: 241 PPCTENVMWTM 252
           PPC+ENV+WT+
Sbjct: 246 PPCSENVIWTI 227

[Close]

BLAST of Bhi08G000177 vs. ExPASy Swiss-Prot
Match: Q9FM99 (Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3)

HSP 1 Score: 209.9 bits (533), Expect = 3.3e-53
Identity = 104/229 (45.41%), Postives = 136/229 (59.39%), Query Frame = 0


Query: 23  EVDDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLMQSPIDLLHERVEVISHLGDLKINY 82
           EV+DE  F YE  G KGP  WG L AEW  C +G MQSPIDL  + V V +  G L+  Y
Sbjct: 133 EVEDETEFSYETKGNKGPAKWGTLDAEWKMCGIGKMQSPIDLRDKNVVVSNKFGLLRSQY 192

Query: 83  KSSNATLKNRGHDMMVILIVLKSLSNIRSIISTSHIYKRKVFVVIQMKWVDGGGYMEMNG 142
             SN T+KNRGHD+M                             ++ K  + G  + + G
Sbjct: 193 LPSNTTIKNRGHDIM-----------------------------LKFKGGNKGIGVTIRG 252

Query: 143 TQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKGD 202
           T+Y+L+Q HWHSPSEHTI+GK+F LE HLVHES +   +V+  LY +G  D FL +++  
Sbjct: 253 TRYQLQQLHWHSPSEHTINGKRFALEEHLVHESKDKRYAVVAFLYNLGASDPFLFSLEKQ 312

Query: 203 LEMVSDTHEEERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTM 252
           L+ ++DTH  E  +GIIDP  L   SK YYRY GSLT PPC+ENV+W++
Sbjct: 313 LKKITDTHASEEHVGIIDPKKLSFESKHYYRYSGSLTAPPCSENVIWSV 332

[Close]

BLAST of Bhi08G000177 vs. ExPASy TrEMBL
Match: A0A6J1I0A8 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469326 PE=3 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 1.3e-108
Identity = 190/254 (74.80%), Postives = 208/254 (81.89%), Query Frame = 0


Query: 1   MGKLLCSFFFSILLLSFKARSQEVDDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLMQS 60
           M KLLCSFFF  LLLSFKA+SQEV+DER FDYE DG KGP HWGELR EWH CK GLMQS
Sbjct: 1   MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60

Query: 61  PIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMVILIVLKSLSNIRSIISTSHIYK 120
           PID+LHERVEVISHLGDLKINYK SNATLKNRGHDMM                       
Sbjct: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMM----------------------- 120

Query: 121 RKVFVVIQMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMI 180
                   MKW++GGG+ME+NG QYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGM+
Sbjct: 121 --------MKWIEGGGFMEVNGMQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMV 180

Query: 181 SVIGILYQIGEPDYFLSTIKGDLEMVSDTHEEERVIGIIDPNLLKMGSKQYYRYIGSLTV 240
           SVIGILYQIGEPDYFLSTIK  LE+VSD+HEEER+IG+IDP L+K+GSK+YYRY+GSLTV
Sbjct: 181 SVIGILYQIGEPDYFLSTIKDHLEVVSDSHEEERIIGVIDPTLIKIGSKRYYRYMGSLTV 223

Query: 241 PPCTENVMWTMIHQ 255
           PPCTE+VMWTMIH+
Sbjct: 241 PPCTESVMWTMIHE 223

[Close]

BLAST of Bhi08G000177 vs. ExPASy TrEMBL
Match: A0A6J1HPL0 (Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111464882 PE=3 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 2.2e-108
Identity = 191/254 (75.20%), Postives = 208/254 (81.89%), Query Frame = 0


Query: 1   MGKLLCSFFFSILLLSFKARSQEVDDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLMQS 60
           M KLLCSFFF ILLLSFKA+SQEV+DER FDYE DG KGP HWGELR EWH CK GLMQS
Sbjct: 1   MEKLLCSFFFCILLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60

Query: 61  PIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMVILIVLKSLSNIRSIISTSHIYK 120
           PID+LHERVEVISHLGDLKINYK SNATLKNRGHDMM                       
Sbjct: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMM----------------------- 120

Query: 121 RKVFVVIQMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMI 180
                   MKWV+GGG+ME+NG QYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGM+
Sbjct: 121 --------MKWVEGGGFMEVNGMQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMV 180

Query: 181 SVIGILYQIGEPDYFLSTIKGDLEMVSDTHEEERVIGIIDPNLLKMGSKQYYRYIGSLTV 240
           SVIGILYQIGEPDYFLSTIK  LE+VS +HEEER+IG+IDP L+K+GSK+YYRY+GSLTV
Sbjct: 181 SVIGILYQIGEPDYFLSTIKDHLEVVSGSHEEERIIGVIDPTLIKIGSKRYYRYMGSLTV 223

Query: 241 PPCTENVMWTMIHQ 255
           PPCTE+VMWTMIH+
Sbjct: 241 PPCTESVMWTMIHE 223

[Close]

BLAST of Bhi08G000177 vs. ExPASy TrEMBL
Match: A0A6J1DG52 (Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111020526 PE=3 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.9e-107
Identity = 192/254 (75.59%), Postives = 204/254 (80.31%), Query Frame = 0


Query: 1   MGKLLCSFFFSILLLSFKARSQEVDDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLMQS 60
           MGKLLCSFFF ILLLSFKA SQEVDDER FDYE DGEKGP HWGEL+AEWH CK G MQS
Sbjct: 1   MGKLLCSFFFCILLLSFKAASQEVDDEREFDYERDGEKGPSHWGELKAEWHKCKAGSMQS 60

Query: 61  PIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMVILIVLKSLSNIRSIISTSHIYK 120
           PIDL H+RVEVISHLGDLK NY  SNAT+KNRGHD+M                       
Sbjct: 61  PIDLYHQRVEVISHLGDLKTNYIPSNATIKNRGHDIM----------------------- 120

Query: 121 RKVFVVIQMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMI 180
                   MKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGK FDLEAHLVHESS G+I
Sbjct: 121 --------MKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKNFDLEAHLVHESSTGII 180

Query: 181 SVIGILYQIGEPDYFLSTIKGDLEMVSDTHEEERVIGIIDPNLLKMGSKQYYRYIGSLTV 240
           S IGILYQIGEPDYFLST+K  LE++SDTHEEER IGIIDP LLK+GSKQYYRYIGSLTV
Sbjct: 181 SAIGILYQIGEPDYFLSTMKDHLEVISDTHEEERAIGIIDPTLLKIGSKQYYRYIGSLTV 223

Query: 241 PPCTENVMWTMIHQ 255
           PPCTENV+WTMI++
Sbjct: 241 PPCTENVIWTMIYE 223

[Close]

BLAST of Bhi08G000177 vs. ExPASy TrEMBL
Match: A0A6J1DHJ2 (Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111020525 PE=3 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.9e-104
Identity = 186/254 (73.23%), Postives = 202/254 (79.53%), Query Frame = 0


Query: 1   MGKLLCSFFFSILLLSFKARSQEVDDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLMQS 60
           MGK LCSFFF ILLLSFKA SQEV++ER FDYE DGE+GP HWG+LR EW  CK G MQS
Sbjct: 1   MGKFLCSFFFCILLLSFKAASQEVENEREFDYEDDGERGPSHWGDLRPEWQKCKAGFMQS 60

Query: 61  PIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMVILIVLKSLSNIRSIISTSHIYK 120
           PIDLLH+RVEVISHLGDL+ NY  SNAT+KNRGHD+M                       
Sbjct: 61  PIDLLHQRVEVISHLGDLETNYIPSNATIKNRGHDIM----------------------- 120

Query: 121 RKVFVVIQMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMI 180
                   MKWVDGGGYMEMNG QYKLKQCHWHSPSEHTIDGK FDLEAHLVHESS G+I
Sbjct: 121 --------MKWVDGGGYMEMNGXQYKLKQCHWHSPSEHTIDGKNFDLEAHLVHESSTGII 180

Query: 181 SVIGILYQIGEPDYFLSTIKGDLEMVSDTHEEERVIGIIDPNLLKMGSKQYYRYIGSLTV 240
           SVIGILYQIGEPDYFLST+K  LE++SDTHEEER+IGIIDP LLK+GSKQYYRYIGSLTV
Sbjct: 181 SVIGILYQIGEPDYFLSTMKDHLEVISDTHEEERIIGIIDPTLLKIGSKQYYRYIGSLTV 223

Query: 241 PPCTENVMWTMIHQ 255
           PPCTENV+WTMI +
Sbjct: 241 PPCTENVIWTMIRE 223

[Close]

BLAST of Bhi08G000177 vs. ExPASy TrEMBL
Match: A0A5D3DDX0 (Carbonic anhydrase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold836G00290 PE=3 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 1.3e-103
Identity = 183/230 (79.57%), Postives = 193/230 (83.91%), Query Frame = 0


Query: 25  DDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLMQSPIDLLHERVEVISHLGDLKINYKS 84
           +DERGFDYE DGEKGP+HWGELRAEWH CK GLMQSPIDLLHERVEVISHLGDL+INYKS
Sbjct: 6   EDERGFDYERDGEKGPVHWGELRAEWHKCKGGLMQSPIDLLHERVEVISHLGDLQINYKS 65

Query: 85  SNATLKNRGHDMMVILIVLKSLSNIRSIISTSHIYKRKVFVVIQMKWVDGGGYMEMNGTQ 144
           SNATLKNRGHDMM                               MKWVDGGGYMEMNGTQ
Sbjct: 66  SNATLKNRGHDMM-------------------------------MKWVDGGGYMEMNGTQ 125

Query: 145 YKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKGDLE 204
           YKLKQCHWHSPSEHTIDGK FDLEAHLVHESSNGM+SVIGILYQIGEPDYFLSTIK DLE
Sbjct: 126 YKLKQCHWHSPSEHTIDGKNFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKEDLE 185

Query: 205 MVSDTHEEERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQ 255
           + S+THEEERV+GIIDPN+LKMGSKQYYRYIGSLTVPPCTE+VMWT+IHQ
Sbjct: 186 VASNTHEEERVVGIIDPNILKMGSKQYYRYIGSLTVPPCTEDVMWTIIHQ 204

The following BLAST results are available for this feature:

BLAST of Bhi08G000177 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G08080.1	4.3e-64	45.31	alpha carbonic anhydrase 7	[more]
AT2G28210.1	1.3e-60	46.29	alpha carbonic anhydrase 2	[more]
AT1G08065.1	2.7e-58	43.03	alpha carbonic anhydrase 5	[more]
AT4G21000.1	1.3e-49	41.13	alpha carbonic anhydrase 6	[more]
AT4G20990.1	2.3e-49	41.74	alpha carbonic anhydrase 4	[more]

BLAST of Bhi08G000177 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L817	6.0e-63	45.31	Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1	[more]
F4IHR4	2.5e-61	44.98	Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2	[more]
Q84UV8	5.7e-61	49.60	Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS...	[more]
F4HUC4	3.8e-57	43.03	Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1	[more]
Q9FM99	3.3e-53	45.41	Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3	[more]

BLAST of Bhi08G000177 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I0A8	1.3e-108	74.80	Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469326 PE=3 SV=1	[more]
A0A6J1HPL0	2.2e-108	75.20	Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111464882 PE=3 SV=1	[more]
A0A6J1DG52	1.9e-107	75.59	Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111020526 PE=3 SV=1	[more]
A0A6J1DHJ2	1.9e-104	73.23	Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111020525 PE=3 SV=1	[more]
A0A5D3DDX0	1.3e-103	79.57	Carbonic anhydrase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold836G0...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001148	Alpha carbonic anhydrase domain	SMART	SM01057	Carb_anhydrase_2a	coord: 32..254 e-value: 2.7E-34 score: 129.9
IPR001148	Alpha carbonic anhydrase domain	PFAM	PF00194	Carb_anhydrase	coord: 31..253 e-value: 3.1E-32 score: 112.2
IPR001148	Alpha carbonic anhydrase domain	PROSITE	PS51144	ALPHA_CA_2	coord: 28..254 score: 38.541103
IPR036398	Alpha carbonic anhydrase domain superfamily	GENE3D	3.10.200.10	Alpha carbonic anhydrase	coord: 27..254 e-value: 1.6E-59 score: 203.2
IPR036398	Alpha carbonic anhydrase domain superfamily	SUPERFAMILY	51069	Carbonic anhydrase	coord: 29..252
None	No IPR available	PANTHER	PTHR18952:SF239	BIFUNCTIONAL MONODEHYDROASCORBATE REDUCTASE AND CARBONIC ANHYDRASE NECTARIN-3	coord: 129..251
None	No IPR available	PANTHER	PTHR18952:SF239	BIFUNCTIONAL MONODEHYDROASCORBATE REDUCTASE AND CARBONIC ANHYDRASE NECTARIN-3	coord: 18..98
IPR023561	Carbonic anhydrase, alpha-class	PANTHER	PTHR18952	CARBONIC ANHYDRASE	coord: 129..251
IPR023561	Carbonic anhydrase, alpha-class	PANTHER	PTHR18952	CARBONIC ANHYDRASE	coord: 18..98
IPR018338	Carbonic anhydrase, alpha-class, conserved site	PROSITE	PS00162	ALPHA_CA_1	coord: 156..172
IPR041891	Carbonic anhydrase, prokaryotic-like	CDD	cd03124	alpha_CA_prokaryotic_like	coord: 39..251 e-value: 5.56742E-77 score: 230.236

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi08M000177	Bhi08M000177	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0004089	carbonate dehydratase activity
molecular_function	GO:0008270	zinc ion binding