Bhi07G001523 (gene) Wax gourd (B227) v1

Overview


Name	Bhi07G001523
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	caffeoyl-CoA O-methyltransferase-like
Location	chr7: 51281091 .. 51289676 (+)
RNA-Seq Expression	Bhi07G001523
Synteny	Bhi07G001523

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTATTCCAAGCATCCAAGCGCCTTCATTACAGTTCAGCTTATGGCGGACGATTCCCCCAAGAAGAACATCCTCCAGCACCGCTTTCTCCAGGTTCTCTCCTATTCCAATCTCAACTTTGTTCTTGTTTTCACTCCGATTCCTGAACTAATTCATTTATCCCTGCGCTGAACCCTGATGATTGCGTTAATTACTACCGACGCTACAGAGTAACTAACATAATCCTTCAATTCTGATTACGTTGATTACTCGCGATTACGCCACAGATTAATTAAACTCAACCATCCTTGAAATTCAATCAAGAGCTATTTAATTTGAAAAGAAAAATGACTGACGTTAATTTCGAAGTTGAAACACATGAATTTGATTGCAGTATATACTCGAAGGCAACGCCTATCCTAGAGAGCACGAACAGTTGAAGAAACTCAGGGAATCCACCTTCGAGAAGTTTGACAAGCGCAGGTGAGTTTCCGACTCTTCAATCGGTTTGATTCAATTTTTGAATGATCGATCGATTTGGATGTATTTTGTCTGATAATCGTATAAGATCAGAGTGGTTAATAGTTTAGAATTTTAATTTTGGGGATTGAGTTACAGGAGTTTGATGATTGTACCAGCAGATGAAGGATTGTTTCTATCAATGCTTTTGAAATTGATGAACGCAAAGAAAACTATAGAGATCGGTGTATTCACTGGCTATTCGCTCCTCACCACGGCACTTGCACTGCCCGCCGATGGCCAGGTAGTCACGGCTTAATTTCCCCTTAATCTCCATCGTCACTGCACTTGCACTGCCCGCAAATTCGCCATCGGCTACGGCTTAATTAATTTTCATTTGATTACGTGCGATTGGCAAATGGATTTCTGATTTTTTCTCTCTTTTAAATATACATTATCTTCTATCATCAAGTTAAACAAGAAAGTTTCCTTTTATCATTTTTTTTTCATATAATTATTTTCAGTATTTCGTTCATGCTTCAGAGTCTTTGAACTTTGAACATAAATGATAAACTAGTCAATTCCAAAATTGTTTAAATAGTCAGAGACGTAATCATTAAGTGGCTGTTTGTGAGTCATCTAAATTAGCCCAATTCCAAAATTTCAAAATGTGACATTCACAGATTCACTTTTGTATACATGAATAAAAGTAAACAATAAAGTAAGATAATTAGAGGTGGAAGGGATGTTTATAGTTTTAACTTTTAAAAATTTTAAACCAAAAATCAAATGGTTCGGGACCTAATCATTTGGTTTGGACTTGATCATTTGGTTTTTAAAAATTAAGCCTCTAAAATCCACTTCCATCTATTGGTTTCAATGTTTTGTTGTTGTCTATACTTTAGAAATGTTTTCAAAATTAAAGACAAATGGAAACTAAAAAATAAAAAATAAAAAATGTAGTTTTCAAAAATTGGCTAAGAATTCAAATACTTACAAAGTGTGAAAATTGAGAGTTAGAAACAAGGAGAAAACAAACATAAATTTTAAAAACCCAAAATCAAAGTTCTATAATTTAAAACTATTTTGTTTTTTGTTTTTGAAAATTAGATACATTTTCTCACCATTTCTTAGCTTATTTGCAACTTTTTTTAAGTGCAAAAGTTTCACATAAAAAATTTAAAGATGAGAGAAATATTTATAGACTTAATTATAAAAGTGAAAAAAATTTAAAACCAAATAGTCAAGAAGATCTTAAACTAAAAGTTTCATCGATGGAAACAAAAAAAAAAAAAAAAAAAAAGTTTGAGATTAAAAAACTAAATAGTCACCAAGATGTTAGAGGGGTTTTTCATAAAAATGGTTGAGATAAACAATTTTTAAAAAGTGAGGGATCTATTAATGCACCAAAAATTAGAAGGTGGAGTGCATTTACTTTACATGAATTTGAGAAGATATATTATCAACTTTATTATTAAAATTCATATAGTTATTGTTTTATTTATTTATTTTACCTGGAATGTGTAGATAACAGCAATTGATGTGAATAGACAATCATTCGAATTTGGTTTGCCATTTATGAAAAAGGCAGGAGTTGATCATAAGATCAATTTTGTGGAATCTGAAGCATTGGTTGCCCTAAATGACCTCCTCAATGATGTAAGTGATTGAAAGTTGCATTTTCTAAACATGCTTTTCTATTCTTCAGATTACAAATTTAGTACACAAAAATATTTGGGTGATGTTACCCTATTTTTTGTTTACTGCTATTTAGTTTTATTTCCTATTTTTGCAATCAAATTAGTTACTTACGGATTCCTATAACAAACGAATTTGAAGTAATGACTTGGTTTTAGTGGTATTTAGGTAGTGGGTTGTTTTAAATTTATAATCAAATTTGAAAAAAAGTAGAGAGACGGATAAGATTTTTTTTAATTTTTTATTTAGTTATGGGTTCTTTTATTTTTTATGTATCTATAAATAAAGGGGTGCATAAAATATTCCTCTTTTTAAAATAAAGGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAGTTATGTGTATTAAATGTTTTATTATAAAAAAGTGATCATTAGTATTTTTTTCGATAAGTGATGAATAATCGATAAATATAGTCATTTAGTATATATATATCTCATTCAGTAAGATAATTCATCTGTTAAAATAAATACATGTCAAATTATCAATGTCAACCTAGGTCAATTAAAACACAATTCTAACATTATTTTAAAGTACAAAACTTATTTATGAATTAAAAGCTCAAATCTTCATTTCATGTTTTTAAATTATATTCTTTATTAATATTTTATTTATTTTAAACAATCCATGATGAAATAGAAACTTGGATATAAGAAAATTTAGCCTATTCATTTATGTTTGTTTATTATTACTTTTATTGAGGAATACATACTATAGCTGAGTCAACAAAATTGAGTTAATATAAGATACGACTAACTTGAACGATGATAGTCAAAGGAAAATTAGAACAAGAATCATATTTGGTATATAAGTACATTAACCACTTTTAAAGAGAAAGCTTGCTAAAGTTGTAGGTAACTTTTTCTACTAAAATAAATAAATGAACACAACGTATGATATATCTAATAATATCTAAAAATTAATGGAGAATAGTTTGATGAAAATTTTATTCAGGAAGAAATTAAATATGATATTATTGTTTCTTTGAAACAGGGCAAAGAAGAAGAGTTTGATTTTGCATTTGTTGATGCCAAAAAATCAGAATACATTAAATATCACGAGTTGCTTCTAAAACTAGTGAAGGTAGGTGGAGTAATTGCCTATGATAACACTTTGTGGTTTGGATCAGTGGCACTAAGCGATGATGACGTTCATGAAGATTTGAAGGAGAACAAAACTCATCTGCAACAACTCAATGCTTTTCTAGTAAATGACATGCGGGTGGAGATAGCTATCATCTCCATTGGAGATGGTGTCACTCTTTGCCGACGTATCAAATAATTTAAGCTTTGAATGTTTCAACGCTATGGTGTCACTCTTTGCCAACGAATATTGCTCCTTGGTTCTTATTTTATTACATTGTCATTGTCAACGAGAAATAATTAATACTTCACAATTATTATACTTCATTAGTAGTTAAAAGTCTTTTCATTGTACCTCGAAAAAATTTAATTATATTTATGGACAATTATTATACTTTCATTAGTAGTTAAAAGTCTTTCCCTCAATTTCGACCAA

mRNA sequence

CTTTATTCCAAGCATCCAAGCGCCTTCATTACAGTTCAGCTTATGGCGGACGATTCCCCCAAGAAGAACATCCTCCAGCACCGCTTTCTCCAGTATATACTCGAAGGCAACGCCTATCCTAGAGAGCACGAACAGTTGAAGAAACTCAGGGAATCCACCTTCGAGAAGTTTGACAAGCGCAGGAGTTTGATGATTGTACCAGCAGATGAAGGATTGTTTCTATCAATGCTTTTGAAATTGATGAACGCAAAGAAAACTATAGAGATCGGTGTATTCACTGGCTATTCGCTCCTCACCACGGCACTTGCACTGCCCGCCGATGGCCAGATAACAGCAATTGATGTGAATAGACAATCATTCGAATTTGGTTTGCCATTTATGAAAAAGGCAGGAGTTGATCATAAGATCAATTTTGTGGAATCTGAAGCATTGGTTGCCCTAAATGACCTCCTCAATGATGGCAAAGAAGAAGAGTTTGATTTTGCATTTGTTGATGCCAAAAAATCAGAATACATTAAATATCACGAGTTGCTTCTAAAACTAGTGAAGGTAGGTGGAGTAATTGCCTATGATAACACTTTGTGGTTTGGATCAGTGGCACTAAGCGATGATGACGTTCATGAAGATTTGAAGGAGAACAAAACTCATCTGCAACAACTCAATGCTTTTCTAGTAAATGACATGCGGGTGGAGATAGCTATCATCTCCATTGGAGATGGTGTCACTCTTTGCCGACGTATCAAATAATTTAAGCTTTGAATGTTTCAACGCTATGGTGTCACTCTTTGCCAACGAATATTGCTCCTTGGTTCTTATTTTATTACATTGTCATTGTCAACGAGAAATAATTAATACTTCACAATTATTATACTTCATTAGTAGTTAAAAGTCTTTTCATTGTACCTCGAAAAAATTTAATTATATTTATGGACAATTATTATACTTTCATTAGTAGTTAAAAGTCTTTCCCTCAATTTCGACCAA

Coding sequence (CDS)

ATGGCGGACGATTCCCCCAAGAAGAACATCCTCCAGCACCGCTTTCTCCAGTATATACTCGAAGGCAACGCCTATCCTAGAGAGCACGAACAGTTGAAGAAACTCAGGGAATCCACCTTCGAGAAGTTTGACAAGCGCAGGAGTTTGATGATTGTACCAGCAGATGAAGGATTGTTTCTATCAATGCTTTTGAAATTGATGAACGCAAAGAAAACTATAGAGATCGGTGTATTCACTGGCTATTCGCTCCTCACCACGGCACTTGCACTGCCCGCCGATGGCCAGATAACAGCAATTGATGTGAATAGACAATCATTCGAATTTGGTTTGCCATTTATGAAAAAGGCAGGAGTTGATCATAAGATCAATTTTGTGGAATCTGAAGCATTGGTTGCCCTAAATGACCTCCTCAATGATGGCAAAGAAGAAGAGTTTGATTTTGCATTTGTTGATGCCAAAAAATCAGAATACATTAAATATCACGAGTTGCTTCTAAAACTAGTGAAGGTAGGTGGAGTAATTGCCTATGATAACACTTTGTGGTTTGGATCAGTGGCACTAAGCGATGATGACGTTCATGAAGATTTGAAGGAGAACAAAACTCATCTGCAACAACTCAATGCTTTTCTAGTAAATGACATGCGGGTGGAGATAGCTATCATCTCCATTGGAGATGGTGTCACTCTTTGCCGACGTATCAAATAA

Protein sequence

MADDSPKKNILQHRFLQYILEGNAYPREHEQLKKLRESTFEKFDKRRSLMIVPADEGLFLSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRQSFEFGLPFMKKAGVDHKINFVESEALVALNDLLNDGKEEEFDFAFVDAKKSEYIKYHELLLKLVKVGGVIAYDNTLWFGSVALSDDDVHEDLKENKTHLQQLNAFLVNDMRVEIAIISIGDGVTLCRRIK

Homology

[Close]

BLAST of Bhi07G001523 vs. TAIR 10
Match: AT1G67980.1 (caffeoyl-CoA 3-O-methyltransferase )

HSP 1 Score: 276.2 bits (705), Expect = 2.5e-74
Identity = 145/234 (61.97%), Postives = 183/234 (78.21%), Query Frame = 0


Query: 1   MADDSPKKNILQHRFL-QYILEGNAYPREHEQLKKLRESTFEKFDKRRSLMIVPADEGLF 60
           MA++ P K IL+   L QYI+E +AYPREHE LK+LR++T +K+    S M VP DEG F
Sbjct: 1   MANEIPTKGILKSEALKQYIMETSAYPREHELLKELRKATVQKYG-NLSEMEVPVDEGHF 60

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRQSFEFGLPFMKKAGVD 120
           LSML+K+MNAK TIEIGVFTGYSLLTTALALP DG+ITAID++++++E GL F+KKAGVD
Sbjct: 61  LSMLVKIMNAKNTIEIGVFTGYSLLTTALALPEDGRITAIDIDKEAYEVGLEFIKKAGVD 120

Query: 121 HKINFVESEALVALNDLLNDGKEEEFDFAFVDAKKSEYIKYHELLLKLVKVGGVIAYDNT 180
           HKINF+ S+ L AL+ L+ND  + EFDFAF DA KS Y+ +HE LLKLVKVGG+IA+DNT
Sbjct: 121 HKINFIHSDGLKALDQLVND--KCEFDFAFADADKSSYVNFHERLLKLVKVGGIIAFDNT 180

Query: 181 LWFGSVALSDDDVHEDLKENKTHLQQLNAFLVNDMRVEIAIISIGDGVTLCRRI 234
           LWFG VA  +D V E ++E +  L + N  L  D RVE++ ISIGDG+TLCRR+
Sbjct: 181 LWFGFVAEDEDGVPEHMREYRAALIEFNKKLALDPRVEVSQISIGDGITLCRRL 231

[Close]

BLAST of Bhi07G001523 vs. TAIR 10
Match: AT1G67990.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 259.2 bits (661), Expect = 3.1e-69
Identity = 135/229 (58.95%), Postives = 175/229 (76.42%), Query Frame = 0


Query: 6   PKKNILQHRFL-QYILEGNAYPREHEQLKKLRESTFEKFDKRRSLMIVPADEGLFLSMLL 65
           P K IL+   L QYI+E  AYPREHE LK+LRE+T +++    S M VP DE LFLSML+
Sbjct: 6   PDKGILKSEALKQYIMETTAYPREHELLKELREATIQRYG-NLSEMGVPVDESLFLSMLV 65

Query: 66  KLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRQSFEFGLPFMKKAGVDHKINF 125
           K++NAK TIEIGVFTGYSL T ALALP DG+ITAID+++  +  GL FMKKAGVDHKINF
Sbjct: 66  KIINAKNTIEIGVFTGYSLFTVALALPEDGRITAIDIDQAGYNLGLEFMKKAGVDHKINF 125

Query: 126 VESEALVALNDLLNDGKEEEFDFAFVDAKKSEYIKYHELLLKLVKVGGVIAYDNTLWFGS 185
           ++S+A+  L+ LLN G+++E+DFAFVDA K+ Y+ + E LLKLVKVGG+IA+DNTLWFG+
Sbjct: 126 IQSDAVRGLDQLLN-GEKQEYDFAFVDADKTNYVYFLEKLLKLVKVGGIIAFDNTLWFGT 185

Query: 186 VALSDDDVHEDLKENKTHLQQLNAFLVNDMRVEIAIISIGDGVTLCRRI 234
           +   +++V   ++  +  L + N  L  D RVEIA ISIGDG+TLCRR+
Sbjct: 186 LIQKENEVPGHMRAYREALLEFNKILARDPRVEIAQISIGDGLTLCRRL 232

[Close]

BLAST of Bhi07G001523 vs. TAIR 10
Match: AT1G24735.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 258.5 bits (659), Expect = 5.3e-69
Identity = 136/229 (59.39%), Postives = 170/229 (74.24%), Query Frame = 0


Query: 6   PKKNILQHRFL-QYILEGNAYPREHEQLKKLRESTFEKFDKRRSLMIVPADEGLFLSMLL 65
           P K IL+   L +YI E  AYPREHEQLKKLRE+T  K+    S M VP DEG FLSMLL
Sbjct: 12  PPKGILKSDALKKYIFETTAYPREHEQLKKLREATVLKYG-NLSEMEVPVDEGHFLSMLL 71

Query: 66  KLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRQSFEFGLPFMKKAGVDHKINF 125
           K+MNAKKTIE+GVFTGYSLLTTALALP DG +T ID++++++E GL F+K AGV HKINF
Sbjct: 72  KIMNAKKTIELGVFTGYSLLTTALALPHDGHVTGIDIDKEAYEMGLEFIKNAGVHHKINF 131

Query: 126 VESEALVALNDLLNDGKEEEFDFAFVDAKKSEYIKYHELLLKLVKVGGVIAYDNTLWFGS 185
           + S+ L AL+++L++  + EFDFAFVDA K  Y   HE L+KLVKVGGVIA+DNTLW G 
Sbjct: 132 IHSDCLQALDNMLSENPKPEFDFAFVDADKPNYANMHERLMKLVKVGGVIAFDNTLWSGF 191

Query: 186 VALSDDDVHEDLKENKTHLQQLNAFLVNDMRVEIAIISIGDGVTLCRRI 234
           VA  +++V   ++ N+     LN  L  D  VE++ +SIGDGVTLCRR+
Sbjct: 192 VAEKEENVPVHMRVNRKAFLDLNKRLAADPHVEVSQVSIGDGVTLCRRL 239

[Close]

BLAST of Bhi07G001523 vs. TAIR 10
Match: AT1G24735.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 244.2 bits (622), Expect = 1.0e-64
Identity = 138/279 (49.46%), Postives = 172/279 (61.65%), Query Frame = 0


Query: 6   PKKNILQHRFL-QYILEGNAYPREHEQLKKLRESTFEKFDK------------------- 65
           P K IL+   L +YI E  AYPREHEQLKKLRE+T  K+                     
Sbjct: 12  PPKGILKSDALKKYIFETTAYPREHEQLKKLREATVLKYGNLYTVLLYDHTLILSNNNCK 71

Query: 66  -------------------------------RRSLMIVPADEGLFLSMLLKLMNAKKTIE 125
                                          RRS M VP DEG FLSMLLK+MNAKKTIE
Sbjct: 72  YHNVTTLDPTKYVYHQFKDYTSNEKMVCACMRRSEMEVPVDEGHFLSMLLKIMNAKKTIE 131

Query: 126 IGVFTGYSLLTTALALPADGQITAIDVNRQSFEFGLPFMKKAGVDHKINFVESEALVALN 185
           +GVFTGYSLLTTALALP DG +T ID++++++E GL F+K AGV HKINF+ S+ L AL+
Sbjct: 132 LGVFTGYSLLTTALALPHDGHVTGIDIDKEAYEMGLEFIKNAGVHHKINFIHSDCLQALD 191

Query: 186 DLLNDGKEEEFDFAFVDAKKSEYIKYHELLLKLVKVGGVIAYDNTLWFGSVALSDDDVHE 234
           ++L++  + EFDFAFVDA K  Y   HE L+KLVKVGGVIA+DNTLW G VA  +++V  
Sbjct: 192 NMLSENPKPEFDFAFVDADKPNYANMHERLMKLVKVGGVIAFDNTLWSGFVAEKEENVPV 251

[Close]

BLAST of Bhi07G001523 vs. TAIR 10
Match: AT4G26220.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 226.5 bits (576), Expect = 2.2e-59
Identity = 124/234 (52.99%), Postives = 158/234 (67.52%), Query Frame = 0


Query: 1   MADDSPKKNILQHRFLQYILEGNAYPREHEQLKKLRESTFEKFDKRRSLMIVPADEGLFL 60
           MA D  K  +      +YILE + YPRE E L++LR  T    +  ++ M    D G  +
Sbjct: 1   MAKDEAKGLLKSEELYKYILETSVYPREPEVLRELRNIT---HNHPQAGMATAPDAGQLM 60

Query: 61  SMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRQSFEFGLPFMKKAGVDH 120
            MLL L+NA+KTIE+GVFTGYSLL TAL LP DG++ AID+NR S+E GLP +KKAGV+H
Sbjct: 61  GMLLNLVNARKTIEVGVFTGYSLLLTALTLPEDGKVIAIDMNRDSYEIGLPVIKKAGVEH 120

Query: 121 KINFVESEALVALNDLLNDG-KEEEFDFAFVDAKKSEYIKYHELLLKLVKVGGVIAYDNT 180
           KI+F ESEAL AL++LLN+   E  FDFAFVDA K  Y  YHE L++L+KVGG+I YDNT
Sbjct: 121 KIDFKESEALPALDELLNNKVNEGGFDFAFVDADKLNYWNYHERLIRLIKVGGIIVYDNT 180

Query: 181 LWFGSVALSDDDVHEDLKENKTHLQQLNAFLVNDMRVEIAIISIGDGVTLCRRI 234
           LW GSVA  D    E   E K    +LN  L  D RV+I+  ++GDG+T+CRR+
Sbjct: 181 LWGGSVAEPDSSTPEWRIEVKKATLELNKKLSADQRVQISQAALGDGITICRRL 231

[Close]

BLAST of Bhi07G001523 vs. ExPASy Swiss-Prot
Match: C7AE94 (Flavonoid 3',5'-methyltransferase OS=Vitis vinifera OX=29760 GN=FAOMT PE=1 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 4.9e-75
Identity = 144/235 (61.28%), Postives = 184/235 (78.30%), Query Frame = 0


Query: 1   MADDSPKKNILQHRFLQYILEGNAYPREHEQLKKLRESTFEKFDKRRSLMIVPADEGLFL 60
           M+  S +  +      +Y+LE +AYPREHEQLK LRE+T EK  K  SLM VP DEGLF+
Sbjct: 1   MSSSSHRGILKTEALTKYLLETSAYPREHEQLKGLREATVEK-HKYWSLMNVPVDEGLFI 60

Query: 61  SMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRQSFEFGLPFMKKAGVDH 120
           SMLLK+MNAKKTIE+GVFTGYSLL TALALP DG+I A+D ++++++ G+PF+KKAGV+H
Sbjct: 61  SMLLKIMNAKKTIELGVFTGYSLLATALALPQDGKIIAVDPDKEAYQTGVPFIKKAGVEH 120

Query: 121 KINFVESEALVALNDLLNDGKEE-EFDFAFVDAKKSEYIKYHELLLKLVKVGGVIAYDNT 180
           KINF++S+A+  LNDL+ DGKEE   DFA VDA K  Y+ YHELLLKLV+VGG+IAYDNT
Sbjct: 121 KINFIQSDAMSVLNDLIADGKEEGTLDFAMVDADKENYLNYHELLLKLVRVGGIIAYDNT 180

Query: 181 LWFGSVALSDDDVHEDL-KENKTHLQQLNAFLVNDMRVEIAIISIGDGVTLCRRI 234
           LWFGSVA S+++   D  +  + HL +LN FL +D RVE++ +SIGDGV LCRR+
Sbjct: 181 LWFGSVARSEEEEMMDFERAGRVHLMKLNKFLASDPRVELSHLSIGDGVALCRRL 234

[Close]

BLAST of Bhi07G001523 vs. ExPASy Swiss-Prot
Match: P93711 (Caffeoyl-CoA O-methyltransferase OS=Populus kitakamiensis OX=34292 PE=2 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 1.6e-73
Identity = 149/235 (63.40%), Postives = 183/235 (77.87%), Query Frame = 0


Query: 1   MADDSPKKNILQHRFL-QYILEGNAYPREHEQLKKLRESTFEKFDKRRSLMIVPADEGLF 60
           MA   P K ILQ   L QYI E +AYP EHEQLK+LRE+T +K+    S M VP DEG F
Sbjct: 1   MAFVLPAKGILQSEALKQYIYETSAYPGEHEQLKELREATTKKYGS-LSGMSVPVDEGRF 60

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRQSFEFGLPFMKKAGVD 120
           LSMLLKLMNAK+T+E+GVFTGYSLL+TALALP DGQ+TAID +R ++E GLPF++KAGV+
Sbjct: 61  LSMLLKLMNAKRTLEVGVFTGYSLLSTALALPEDGQVTAIDKDRGAYEIGLPFIQKAGVE 120

Query: 121 HKINFVESEALVALNDLLNDGKEEEFDFAFVDAKKSEYIKYHELLLKLVKVGGVIAYDNT 180
            KINF++SEA   LN++L + K+ EFDFAFVDA KS Y  YHE LLKLVK+GG+IAYDNT
Sbjct: 121 DKINFIQSEAPPILNEMLCNDKQPEFDFAFVDADKSSYKHYHEQLLKLVKIGGIIAYDNT 180

Query: 181 LWFGSVALS-DDDVHEDLKENKTHLQQLNAFLVNDMRVEIAIISIGDGVTLCRRI 234
           LW+G VA   DD+V E L+  +T + + N  L +D+RVEI+ ISIGDGVTLCRR+
Sbjct: 181 LWYGLVAKEVDDEVPEPLRMVRTVIMEFNKLLSSDLRVEISQISIGDGVTLCRRL 234

[Close]

BLAST of Bhi07G001523 vs. ExPASy Swiss-Prot
Match: Q9C9W3 (Putative caffeoyl-CoA O-methyltransferase At1g67980 OS=Arabidopsis thaliana OX=3702 GN=At1g67980 PE=2 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 3.5e-73
Identity = 145/234 (61.97%), Postives = 183/234 (78.21%), Query Frame = 0


Query: 1   MADDSPKKNILQHRFL-QYILEGNAYPREHEQLKKLRESTFEKFDKRRSLMIVPADEGLF 60
           MA++ P K IL+   L QYI+E +AYPREHE LK+LR++T +K+    S M VP DEG F
Sbjct: 1   MANEIPTKGILKSEALKQYIMETSAYPREHELLKELRKATVQKYG-NLSEMEVPVDEGHF 60

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRQSFEFGLPFMKKAGVD 120
           LSML+K+MNAK TIEIGVFTGYSLLTTALALP DG+ITAID++++++E GL F+KKAGVD
Sbjct: 61  LSMLVKIMNAKNTIEIGVFTGYSLLTTALALPEDGRITAIDIDKEAYEVGLEFIKKAGVD 120

Query: 121 HKINFVESEALVALNDLLNDGKEEEFDFAFVDAKKSEYIKYHELLLKLVKVGGVIAYDNT 180
           HKINF+ S+ L AL+ L+ND  + EFDFAF DA KS Y+ +HE LLKLVKVGG+IA+DNT
Sbjct: 121 HKINFIHSDGLKALDQLVND--KCEFDFAFADADKSSYVNFHERLLKLVKVGGIIAFDNT 180

Query: 181 LWFGSVALSDDDVHEDLKENKTHLQQLNAFLVNDMRVEIAIISIGDGVTLCRRI 234
           LWFG VA  +D V E ++E +  L + N  L  D RVE++ ISIGDG+TLCRR+
Sbjct: 181 LWFGFVAEDEDGVPEHMREYRAALIEFNKKLALDPRVEVSQISIGDGITLCRRL 231

[Close]

BLAST of Bhi07G001523 vs. ExPASy Swiss-Prot
Match: A0A077EW86 (Norbelladine 4'-O-methyltransferase 2 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=N4OMT2 PE=2 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 3.9e-72
Identity = 147/229 (64.19%), Postives = 179/229 (78.17%), Query Frame = 0


Query: 8   KNILQHR-FLQYILEGNAYPREHEQLKKLRESTFEKFDKRRSLMIVPADEGLFLSMLLKL 67
           KNIL     L+YILE +AYPREHEQLK LRE T EK +   S  +VPADEGLFLSMLLKL
Sbjct: 13  KNILHSEDLLKYILETSAYPREHEQLKGLREVT-EKHE--WSSALVPADEGLFLSMLLKL 72

Query: 68  MNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRQSFEFGLPFMKKAGVDHKINFVE 127
           MNAK+TIEIGV+TGYSLLTTALALP DG+ITAIDVN+  FE GLPF++KAGV+HKINF+E
Sbjct: 73  MNAKRTIEIGVYTGYSLLTTALALPEDGKITAIDVNKSFFEIGLPFIQKAGVEHKINFIE 132

Query: 128 SEALVALNDLLNDGKEEE-FDFAFVDAKKSEYIKYHELLLKLVKVGGVIAYDNTLWFGSV 187
           SEAL  L+ +L + KEE+ +D+AFVDA KS Y  YHE L+KLV++GG I YDNTLW+GSV
Sbjct: 133 SEALPVLDQMLQETKEEDLYDYAFVDADKSNYANYHERLVKLVRIGGAILYDNTLWYGSV 192

Query: 188 ALSD-DDVHEDLKENKTHLQQLNAFLVNDMRVEIAIISIGDGVTLCRRI 234
           A  +   +H + +  +   + LN FL  D RVEI+ +SIGDGVT+CRR+
Sbjct: 193 AYPEYPGLHPEEEVARLSFRNLNTFLAADPRVEISQVSIGDGVTICRRL 238

[Close]

BLAST of Bhi07G001523 vs. ExPASy Swiss-Prot
Match: A0A077ESS0 (Norbelladine 4'-O-methyltransferase 5 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=N4OMT5 PE=2 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 8.6e-72
Identity = 145/229 (63.32%), Postives = 178/229 (77.73%), Query Frame = 0


Query: 7   KKNILQHRFLQYILEGNAYPREHEQLKKLRESTFEKFDKRRSLMIVPADEGLFLSMLLKL 66
           KK +     L+YILE +AYPREHEQLK LRE T EK +   S  +VPADEGLFLSMLLKL
Sbjct: 13  KKILHSEDLLKYILETSAYPREHEQLKGLREVT-EKHE--WSSALVPADEGLFLSMLLKL 72

Query: 67  MNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRQSFEFGLPFMKKAGVDHKINFVE 126
           MNAK+TIEIGV+TGYSLLTTALALP DG+ITAIDVN+  FE GLPF++KAGV+HKINF+E
Sbjct: 73  MNAKRTIEIGVYTGYSLLTTALALPEDGKITAIDVNKSFFEIGLPFIQKAGVEHKINFIE 132

Query: 127 SEALVALNDLLNDGKEEE-FDFAFVDAKKSEYIKYHELLLKLVKVGGVIAYDNTLWFGSV 186
           SEAL  L+ +L + KEE+ +D+AFVDA KS Y  YHE L+KLV++GG I YDNTLW+GSV
Sbjct: 133 SEALPVLDQMLQETKEEDLYDYAFVDADKSNYANYHERLVKLVRIGGAILYDNTLWYGSV 192

Query: 187 ALSD-DDVHEDLKENKTHLQQLNAFLVNDMRVEIAIISIGDGVTLCRRI 234
           A  +   +H + +  +   + LN FL  D RVEI+ +SIGDGVT+CRR+
Sbjct: 193 AYPEYPGLHPEEEVARLSFRNLNTFLAADPRVEISQVSIGDGVTICRRL 238

[Close]

BLAST of Bhi07G001523 vs. ExPASy TrEMBL
Match: A0A6J1DCA7 (flavonoid 3',5'-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111019721 PE=3 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 5.0e-99
Identity = 188/234 (80.34%), Postives = 212/234 (90.60%), Query Frame = 0


Query: 1   MADDSPKKNILQH-RFLQYILEGNAYPREHEQLKKLRESTFEKFDKRRSLMIVPADEGLF 60
           MA+D P KNILQ    LQYILE NAYPREHEQLK+LRE+T  KFDK RS+M VPAD+GLF
Sbjct: 18  MANDFPDKNILQSPALLQYILESNAYPREHEQLKELRETTVGKFDKSRSVMGVPADQGLF 77

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRQSFEFGLPFMKKAGVD 120
           L+ML+KLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDV+R+SFE GLPF+KKAGVD
Sbjct: 78  LAMLVKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVDRKSFEIGLPFIKKAGVD 137

Query: 121 HKINFVESEALVALNDLLNDGKEEEFDFAFVDAKKSEYIKYHELLLKLVKVGGVIAYDNT 180
           HKINFVESEAL+ LNDLL DGKE EFDFAFVDAKKSEYIKYHELLL+LVKVGGVI YDNT
Sbjct: 138 HKINFVESEALIVLNDLLKDGKEGEFDFAFVDAKKSEYIKYHELLLRLVKVGGVIGYDNT 197

Query: 181 LWFGSVALSDDDVHEDLKENKTHLQQLNAFLVNDMRVEIAIISIGDGVTLCRRI 234
           LW+GSVA S++ V  DLK+N+ ++++LNAFLVND RVEIA++SIGDG+TLCRRI
Sbjct: 198 LWYGSVAQSEELVEADLKDNRIYMRELNAFLVNDSRVEIALLSIGDGLTLCRRI 251

[Close]

BLAST of Bhi07G001523 vs. ExPASy TrEMBL
Match: A0A6J1HSF7 (caffeoyl-CoA O-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466357 PE=3 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 1.1e-98
Identity = 187/235 (79.57%), Postives = 206/235 (87.66%), Query Frame = 0


Query: 1   MADDSPKKNILQH-RFLQYILEGNAYPREHEQLKKLRESTFEKFDKRRSLMIVPADEGLF 60
           MADD P KNILQ    LQYILE N+YPREHEQLK+LRE+T  KFDK  S+M VPAD+GLF
Sbjct: 1   MADDCPDKNILQSTALLQYILEANSYPREHEQLKELRETTLRKFDKSLSVMSVPADQGLF 60

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRQSFEFGLPFMKKAGVD 120
           LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNR+SFE GLPF KKAGVD
Sbjct: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRESFEIGLPFFKKAGVD 120

Query: 121 HKINFVESEALVALNDLLNDGKEEEFDFAFVDAKKSEYIKYHELLLKLVKVGGVIAYDNT 180
           HKINFVES ALV LNDL+ND    EFDFAFVDA K EYIKYHELLL+LVKVGG+IAYDNT
Sbjct: 121 HKINFVESMALVVLNDLINDDTNTEFDFAFVDAVKGEYIKYHELLLRLVKVGGIIAYDNT 180

Query: 181 LWFGSVALSDDDVHEDLKENKTHLQQLNAFLVNDMRVEIAIISIGDGVTLCRRIK 235
           LW+GSVA +DD V  DLK N++H+++ NAFLVND RVEIA++SIGDG+TLCRRIK
Sbjct: 181 LWYGSVAQNDDVVPHDLKANRSHIREFNAFLVNDSRVEIALLSIGDGLTLCRRIK 235

[Close]

BLAST of Bhi07G001523 vs. ExPASy TrEMBL
Match: A0A6J1G1C7 (caffeoyl-CoA O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111449784 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 3.2e-98
Identity = 184/235 (78.30%), Postives = 207/235 (88.09%), Query Frame = 0


Query: 1   MADDSPKKNILQH-RFLQYILEGNAYPREHEQLKKLRESTFEKFDKRRSLMIVPADEGLF 60
           MADD P KNILQ    LQYILE N+YPREHEQLK+LRE+T  KFDK  S+M VP D+GLF
Sbjct: 1   MADDCPDKNILQSTALLQYILEANSYPREHEQLKELRETTLRKFDKSLSVMSVPGDQGLF 60

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRQSFEFGLPFMKKAGVD 120
           LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNR SFE GLPF+KKAGVD
Sbjct: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRASFEIGLPFIKKAGVD 120

Query: 121 HKINFVESEALVALNDLLNDGKEEEFDFAFVDAKKSEYIKYHELLLKLVKVGGVIAYDNT 180
           HKINFVES+AL+ LN+L+ND  + EFDFAFVDA K EYIKYHE LL+LVKVGGVIAYDNT
Sbjct: 121 HKINFVESQALIVLNNLINDDAKTEFDFAFVDAVKGEYIKYHEFLLRLVKVGGVIAYDNT 180

Query: 181 LWFGSVALSDDDVHEDLKENKTHLQQLNAFLVNDMRVEIAIISIGDGVTLCRRIK 235
           LW+GSVA SDD V +DLK N++H+++ NAFLVND RVEIA++SIGDG+TLCRR+K
Sbjct: 181 LWYGSVAQSDDVVQDDLKANRSHIREFNAFLVNDSRVEIALLSIGDGLTLCRRVK 235

[Close]

BLAST of Bhi07G001523 vs. ExPASy TrEMBL
Match: A0A6J1G1B9 (flavonoid 3',5'-methyltransferase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449785 PE=3 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 2.7e-97
Identity = 188/235 (80.00%), Postives = 211/235 (89.79%), Query Frame = 0


Query: 1   MADDSPKKNILQ-HRFLQYILEGNAYPREHEQLKKLRESTFEKFDKRRSLMIVPADEGLF 60
           MADDS KKNILQ    LQYILE N+YPREHEQLK+LRE+T E F K RS+M VPAD+GLF
Sbjct: 1   MADDS-KKNILQCPALLQYILEENSYPREHEQLKELRETTLETF-KSRSVMSVPADQGLF 60

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRQSFEFGLPFMKKAGVD 120
           LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAID++R+SFE GLPF+KKAGVD
Sbjct: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDISRESFEVGLPFIKKAGVD 120

Query: 121 HKINFVESEALVALNDLLNDGKEEEFDFAFVDAKKSEYIKYHELLLKLVKVGGVIAYDNT 180
           HKINFVES+AL+ LNDLLNDGKE EFDF FVDA K EYIKYHELL+KLVKVGGVIAYDNT
Sbjct: 121 HKINFVESQALIVLNDLLNDGKEGEFDFVFVDADKGEYIKYHELLMKLVKVGGVIAYDNT 180

Query: 181 LWFGSVALSDDDVHEDLKENKTHLQQLNAFLVNDMRVEIAIISIGDGVTLCRRIK 235
           LWFG+VA SDD V E LK ++ H+++LNAFLVND RVEIA++S+GDG+TLCRRI+
Sbjct: 181 LWFGTVAQSDDVVDEKLKASRNHIRELNAFLVNDSRVEIALLSVGDGITLCRRIE 233

[Close]

BLAST of Bhi07G001523 vs. ExPASy TrEMBL
Match: A0A0A0KTC8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589940 PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.4e-96
Identity = 186/235 (79.15%), Postives = 212/235 (90.21%), Query Frame = 0


Query: 1   MADDSPKKNILQH-RFLQYILEGNAYPREHEQLKKLRESTFEKFDKRRSLMIVPADEGLF 60
           MA+    KNIL+    LQYIL+ NAYPREHEQLK+LRESTF KFDK  S+M VP DEGLF
Sbjct: 1   MANYHSDKNILKSTALLQYILKANAYPREHEQLKELRESTFNKFDKSGSVMNVPVDEGLF 60

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRQSFEFGLPFMKKAGVD 120
           LSMLLKLMNAKKTIE+GV+TGYSLLTTALALPADG+ITAID++R+ FEFGLPF++KAGVD
Sbjct: 61  LSMLLKLMNAKKTIEVGVYTGYSLLTTALALPADGRITAIDMDRKPFEFGLPFIRKAGVD 120

Query: 121 HKINFVESEALVALNDLLNDGKEEEFDFAFVDAKKSEYIKYHELLLKLVKVGGVIAYDNT 180
           HKINFVES+ALVALN+LLNDGK ++FDFAFVDA KSEYI+YHELLLKLVKVGGVIAYDNT
Sbjct: 121 HKINFVESKALVALNNLLNDGK-KDFDFAFVDAMKSEYIEYHELLLKLVKVGGVIAYDNT 180

Query: 181 LWFGSVALSDDDVHEDLKENKTHLQQLNAFLVNDMRVEIAIISIGDGVTLCRRIK 235
           LWFGSVALSD++V E L+E+K H+Q+ NAFLV D RVEIA++SIGDGVTLCRRIK
Sbjct: 181 LWFGSVALSDNEVDEHLRESKVHIQRFNAFLVKDSRVEIALLSIGDGVTLCRRIK 234

The following BLAST results are available for this feature:

BLAST of Bhi07G001523 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G67980.1	2.5e-74	61.97	caffeoyl-CoA 3-O-methyltransferase	[more]
AT1G67990.1	3.1e-69	58.95	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G24735.1	5.3e-69	59.39	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G24735.2	1.0e-64	49.46	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G26220.1	2.2e-59	52.99	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

BLAST of Bhi07G001523 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C7AE94	4.9e-75	61.28	Flavonoid 3',5'-methyltransferase OS=Vitis vinifera OX=29760 GN=FAOMT PE=1 SV=1	[more]
P93711	1.6e-73	63.40	Caffeoyl-CoA O-methyltransferase OS=Populus kitakamiensis OX=34292 PE=2 SV=1	[more]
Q9C9W3	3.5e-73	61.97	Putative caffeoyl-CoA O-methyltransferase At1g67980 OS=Arabidopsis thaliana OX=3...	[more]
A0A077EW86	3.9e-72	64.19	Norbelladine 4'-O-methyltransferase 2 OS=Narcissus aff. pseudonarcissus MK-2014 ...	[more]
A0A077ESS0	8.6e-72	63.32	Norbelladine 4'-O-methyltransferase 5 OS=Narcissus aff. pseudonarcissus MK-2014 ...	[more]

BLAST of Bhi07G001523 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DCA7	5.0e-99	80.34	flavonoid 3',5'-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1HSF7	1.1e-98	79.57	caffeoyl-CoA O-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1G1C7	3.2e-98	78.30	caffeoyl-CoA O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111449...	[more]
A0A6J1G1B9	2.7e-97	80.00	flavonoid 3',5'-methyltransferase-like isoform X1 OS=Cucurbita moschata OX=3662 ...	[more]
A0A0A0KTC8	1.4e-96	79.15	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589940 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002935	Class I-like SAM-dependent O-methyltransferase	PFAM	PF01596	Methyltransf_3	coord: 25..233 e-value: 2.0E-80 score: 268.9
IPR002935	Class I-like SAM-dependent O-methyltransferase	PROSITE	PS51682	SAM_OMT_I	coord: 8..233 score: 59.897808
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 4..234 e-value: 2.9E-69 score: 234.9
None	No IPR available	PANTHER	PTHR10509	O-METHYLTRANSFERASE-RELATED	coord: 8..233
None	No IPR available	PANTHER	PTHR10509:SF80	FLAVONOID 3',5'-METHYLTRANSFERASE-LIKE	coord: 8..233
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 73..176 e-value: 0.00399216 score: 33.9427
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 12..232

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi07M001523	Bhi07M001523	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity