Bhi07G000608 (gene) Wax gourd (B227) v1

Overview


Name	Bhi07G000608
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like
Location	chr7: 26026924 .. 26029854 (+)
RNA-Seq Expression	Bhi07G000608
Synteny	Bhi07G000608

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CACATACTTAGTTCATAGATATAAATTCGTGATTCTATATAATTATTTTTTAAAGTTAATTATCTTATTTACATGTTAAAAAATATAAAATAGGTTAGGTAGGTGTAAACTATTAGGTATCAAAAGATAAAATCAATGTCATATTTACTGATTTCTAATCGAAATTGATATAATTGCAATGAGATTTTATTGATAAATTAATCACAAATATAAGTGGATTGTTTTTGGAGTATTGTGTGATAAACCAGACCACTAACCAGCGTTGCCGATTGCAATTAAGTAAAAAATGTCTTTGCGATTTGGATGTAACGTGTGATAGACCAGGCCACTGGTTCACGATTAGACCCGAAGAAGGTTGAATCCCCAATACCCGAAAATGGCTAAATCCCAATGGCGTTCTATGAAACTTCACTTTATTTGTCCTCCAATGCCGTTTCAGATTTCTACACGCGTCTTCTGAAGCTTTTTCCCATCGGAATCTCTGCAGCAATGGACGTTTTGTCGGCGTTTCTCTTCCCTCCGCCTCCTTCCAAGTTCGTCAACCTCATGACGGTGGTGAGCTCGACCGCGTTGGCCATGGCTGGCATTTCCGAGATTAACGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCGAATACTGCTCCGAAACTTTCATCGGAAGTATCTATGCTTTCGTCCAAATACGGGATGCTCTTGGCTTATACTCCGGCGTTTCTCGCTGGCGCGGCGTCGTTTTGGCTCTTTCCGAGTGCCGATGAGAGGATTCTTCTGCTTAAATCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTTGAGGTACACCTTTTTTTTTTTTTTTTTTTTCCATCTACGCCTTTGTTTGGTTCTGAAATTTCGAATCTGATTCTGGAAACAAGTATAGTAGAATGCAGTTTCTTCTCTGATGTTCTTGAGTAGCTAAATTCATATCCTGGTAAAATTATTTGTTCGAACCGCTGCGAATCTTATTCTTCGAAAATTTCTCGTTTTCTTGTAAACCGTATGAAAAATTCTCGGCGAAGAGAAAATTATGGAGACATCTGGATCTATTCTTCCTTCTCTGCATCTTATCATTCCTCGATTTCAAGTTTGGAACTCTTTTCTTTTTTGTTTACGGGATTGAGAAGACTTCAACCTAACTTGAACTTTCTTTTAATTATTTGTTTATCTTATGAATTTTTTTAGTAAAATAGGATTGGCATCCGAACTACAGAACTTTTTGTCACTGGTTGAATTATTTACTTTGAAGTTTGGATCGTGAACTTGGTTAGTAACCCATCCGCTCATAATTATCCGAGTTCATTAATTATCCTATTTGACTAATTGAATTATCGTTAAGATAATCTGAGTGTACTTTGTATAGAAAAATTGAAATTATTATTATATGAAGTCATGTTTAAGAAAGAAACAAACTACAAGATTCTGTTCAACAGTTCTCCAAAAATTCTATTTCATTAATCACGATATGATTTGCAAGTCCAGTACATTTTATTCGTCTAGCTATTACTTTGCAAACAATTTCAGGATTTTCTGAAACATGTTCTTGTGTTAAATTACCAGCGAACCCAAAACCTTAAGTTGGTGGACTATGTTGATTATACTTATATCAATATTTTACACTATTTCTCAACTGTGAGGTTAGGAATTTGTAGAAAACTCAATAAGTGGTAATCATTTTTCTTTTAAGAATACCGAACTTTTCATTAAGTAAATAAAAAGATACTAATGCTCCAAAATACAAAGTAAAGAAGGCCCTAAGGAAATAGAGTCACAAAAAATATAAAAGATAGAGATCGACTAAGAAAGAAACACGGAGTCTTCCTGATTCTTGTTCAAATCTTGGATGGAATAACCCTTGAAACTTTTAGATTATGAACAAAATGAAGAAAACTTCAAGTGGGCTGAATCAAAGCACTCCAAACATGAGCATGATGAATTTTAAAAATATACATAGGTTTCTTAACAAGTGGTAATCAATGTTAATTGAAAAGGAAATGACATTAAAAAAAGTTCAAACATTTCAAACTTCTTACGTTAATACCATGTTAAATCTTTGCTAAACTGAAAAACTTGGGTGAATGAATGTACTAATCTTTTTGCTCTCTTGGGAATCTTGACAGGTACTGTTTATTCACAAATACAGCAGTAAGATGGTCTTGGTGACAGCAATCCAGATCTCTTTCAGCTACTTCTTGTCCACAGTAATCATGATATACACCCAGCACCTTTCACAAGGGCTTCCAGAGCCACCCATTGAACTGAAAAACATTGGAATTGGTTTGTTTCTAATAGGCATTATTGGGAACTTTTACCATCATTATCTCTTGTCAAAAACCAGAAAAGAGGGAGAAACAAGCTACGAAATTCCCAAAGGAGGCCTCTTCAGCTTTGTCATTTGCCCCCACTATTTGTTTGAAATAATTGAGTTCTTTGGATTTGCTTTCATCTCTCAGGAACTTTATCCTTTGTGTTTCAGCATTGGCACCAGTATTTATTTGGCTGGGAGGAGCTATGCCACTAGGAAATGGTACATCTCCAAGTTTGAAAACTTCCCCAACCATGTCAAAGCCCTGCTCCCTTTTGTCTTTTAGACCCTTTTTTGGGTCTTTTCATAAAGTTACTTGTGTTATACTTTACCCAATTAAGTTAGGCTCATTCTAGTGCTTTAGGCCTTCCCTAGCTTGTTATTCTTTGGGCTCTGTTCTTACAGTTCAATAGGTTTGTTTTTTGGAATCATCCAATGATGTTAAGGTTTATTTTCTTTTTCATCATTATCACTAATATATTACAAGGAAACAAGGTGAATGCTTTTCTTCTAATGAGAAATAAATATGATTAAAATATGTTTCTATATTTTAAAGTTTGTTCAATTTTAGTACTCTAAAAGATGTTATTAAAGTCATTTAAATA

mRNA sequence

CACATACTTAGTTCATAGATATAAATTCGTGATTCTATATAATTATTTTTTAAAGTTAATTATCTTATTTACATGTTAAAAAATATAAAATAGGTTAGGTAGGTGTAAACTATTAGGTATCAAAAGATAAAATCAATGTCATATTTACTGATTTCTAATCGAAATTGATATAATTGCAATGAGATTTTATTGATAAATTAATCACAAATATAAGTGGATTGTTTTTGGAGTATTGTGTGATAAACCAGACCACTAACCAGCGTTGCCGATTGCAATTAAGTAAAAAATGTCTTTGCGATTTGGATGTAACGTGTGATAGACCAGGCCACTGGTTCACGATTAGACCCGAAGAAGGTTGAATCCCCAATACCCGAAAATGGCTAAATCCCAATGGCGTTCTATGAAACTTCACTTTATTTGTCCTCCAATGCCGTTTCAGATTTCTACACGCGTCTTCTGAAGCTTTTTCCCATCGGAATCTCTGCAGCAATGGACGTTTTGTCGGCGTTTCTCTTCCCTCCGCCTCCTTCCAAGTTCGTCAACCTCATGACGGTGGTGAGCTCGACCGCGTTGGCCATGGCTGGCATTTCCGAGATTAACGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCGAATACTGCTCCGAAACTTTCATCGGAAGTATCTATGCTTTCGTCCAAATACGGGATGCTCTTGGCTTATACTCCGGCGTTTCTCGCTGGCGCGGCGTCGTTTTGGCTCTTTCCGAGTGCCGATGAGAGGATTCTTCTGCTTAAATCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTTGAGGTACTGTTTATTCACAAATACAGCAGTAAGATGGTCTTGGTGACAGCAATCCAGATCTCTTTCAGCTACTTCTTGTCCACAGTAATCATGATATACACCCAGCACCTTTCACAAGGGCTTCCAGAGCCACCCATTGAACTGAAAAACATTGGAATTGGTTTGTTTCTAATAGGCATTATTGGGAACTTTTACCATCATTATCTCTTGTCAAAAACCAGAAAAGAGGGAGAAACAAGCTACGAAATTCCCAAAGGAGGCCTCTTCAGCTTTGTCATTTGCCCCCACTATTTGTTTGAAATAATTGAGTTCTTTGGATTTGCTTTCATCTCTCAGGAACTTTATCCTTTGTGTTTCAGCATTGGCACCAGTATTTATTTGGCTGGGAGGAGCTATGCCACTAGGAAATGGTACATCTCCAAGTTTGAAAACTTCCCCAACCATGTCAAAGCCCTGCTCCCTTTTGTCTTTTAGACCCTTTTTTGGGTCTTTTCATAAAGTTACTTGTGTTATACTTTACCCAATTAAGTTAGGCTCATTCTAGTGCTTTAGGCCTTCCCTAGCTTGTTATTCTTTGGGCTCTGTTCTTACAGTTCAATAGGTTTGTTTTTTGGAATCATCCAATGATGTTAAGGTTTATTTTCTTTTTCATCATTATCACTAATATATTACAAGGAAACAAGGTGAATGCTTTTCTTCTAATGAGAAATAAATATGATTAAAATATGTTTCTATATTTTAAAGTTTGTTCAATTTTAGTACTCTAAAAGATGTTATTAAAGTCATTTAAATA

Coding sequence (CDS)

ATGGCGTTCTATGAAACTTCACTTTATTTGTCCTCCAATGCCGTTTCAGATTTCTACACGCGTCTTCTGAAGCTTTTTCCCATCGGAATCTCTGCAGCAATGGACGTTTTGTCGGCGTTTCTCTTCCCTCCGCCTCCTTCCAAGTTCGTCAACCTCATGACGGTGGTGAGCTCGACCGCGTTGGCCATGGCTGGCATTTCCGAGATTAACGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCGAATACTGCTCCGAAACTTTCATCGGAAGTATCTATGCTTTCGTCCAAATACGGGATGCTCTTGGCTTATACTCCGGCGTTTCTCGCTGGCGCGGCGTCGTTTTGGCTCTTTCCGAGTGCCGATGAGAGGATTCTTCTGCTTAAATCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTTGAGGTACTGTTTATTCACAAATACAGCAGTAAGATGGTCTTGGTGACAGCAATCCAGATCTCTTTCAGCTACTTCTTGTCCACAGTAATCATGATATACACCCAGCACCTTTCACAAGGGCTTCCAGAGCCACCCATTGAACTGAAAAACATTGGAATTGGTTTGTTTCTAATAGGCATTATTGGGAACTTTTACCATCATTATCTCTTGTCAAAAACCAGAAAAGAGGGAGAAACAAGCTACGAAATTCCCAAAGGAGGCCTCTTCAGCTTTGTCATTTGCCCCCACTATTTGTTTGAAATAATTGAGTTCTTTGGATTTGCTTTCATCTCTCAGGAACTTTATCCTTTGTGTTTCAGCATTGGCACCAGTATTTATTTGGCTGGGAGGAGCTATGCCACTAGGAAATGGTACATCTCCAAGTTTGAAAACTTCCCCAACCATGTCAAAGCCCTGCTCCCTTTTGTCTTTTAG

Protein sequence

MAFYETSLYLSSNAVSDFYTRLLKLFPIGISAAMDVLSAFLFPPPPSKFVNLMTVVSSTALAMAGISEINGKHLQYSKFWNANTAPKLSSEVSMLSSKYGMLLAYTPAFLAGAASFWLFPSADERILLLKSALTLHFFKRIFEVLFIHKYSSKMVLVTAIQISFSYFLSTVIMIYTQHLSQGLPEPPIELKNIGIGLFLIGIIGNFYHHYLLSKTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFISQELYPLCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF

Homology

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BLAST of Bhi07G000608 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 312.0 bits (798), Expect = 5.2e-85
Identity = 154/268 (57.46%), Postives = 205/268 (76.49%), Query Frame = 0


Query: 34  MDVLSAFLFPPPPSKFVNLMTVVSSTALAMAGISEINGKHLQYSKFWNANTAPKLSSE-V 93
           M+++++F++PPPPS  +N M+VV   ALA  G SEI G HL+YSKF  ++++P+   E  
Sbjct: 1   MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKFGVSSSSPQPQKERF 60

Query: 94  SMLSSKYGMLLAYTPAFLAGAASFWLFPSADERILLLKSALTLHFFKRIFEVLFIHKYSS 153
             +SS+ GMLL YTPAFLA A+SF++ PS D R LLLKSAL LHFFKR+FEVLFIHKYS 
Sbjct: 61  GSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIHKYSG 120

Query: 154 KMVLVTAIQISFSYFLSTVIMIYTQHLSQGLPEPPIELKNIGIGLFLIGIIGNFYHHYLL 213
            M + +A+ IS SYF ST +M+Y+Q+L+ GL EP  ++K  G+ +F++GI+GN YHH LL
Sbjct: 121 GMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLL 180

Query: 214 SKTRKE-GETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFISQELYPLCFSIGTSIYLAG 273
           +K RKE G+  Y+IPKGGLF  +ICPHYLFEI+ F+ F  ISQ +Y   F++GT +YL G
Sbjct: 181 AKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIG 240

Query: 274 RSYATRKWYISKFENFPNHVKALLPFVF 300
           RSYATR WY+SKF++FP H+KAL+PFVF
Sbjct: 241 RSYATRTWYLSKFDDFPKHIKALIPFVF 268

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BLAST of Bhi07G000608 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.5e-12
Identity = 58/209 (27.75%), Postives = 97/209 (46.41%), Query Frame = 0


Query: 109 FLAGAASFW----LFP----SADERILLLKSALTLHFFK-------RIFEVLFIHKYSSK 168
           F+  + + W    LFP    + + + LLL S   +H+F        R+F   F    +  
Sbjct: 54  FVMESPTLWLTLLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKNGF 113

Query: 169 MVLVTAIQISFSYFLSTVIMIYTQHLSQGLPEPPIELKN--IGIGLFLIGIIGNFYHHYL 228
            + + A+  +F+     +   +  H      +         IG+ +F+ G+  N      
Sbjct: 114 PITIAALAFTFNLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRT 173

Query: 229 LSKTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFISQELYPLCFSIGTSIYLAG 288
           L + +KE    Y IP+GG F  V CP+Y  E IE+ G+A ++     + F + T   L  
Sbjct: 174 LVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFP 233

Query: 289 RSYATRKWYISKF-ENFPNHVKALLPFVF 300
           R+ A+ KWYI+KF E +P   KA++PFV+
Sbjct: 234 RARASHKWYIAKFKEEYPKTRKAVIPFVY 262

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BLAST of Bhi07G000608 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.7e-11
Identity = 47/151 (31.13%), Postives = 73/151 (48.34%), Query Frame = 0


Query: 136 HFFKRIFEVLFIHKYSSKMVLVTAIQISFSYFLS--TVIMIYTQHLSQGLPEPPIELKNI 195
           H+FKRI E  F+H++S     +  +  + +Y+ S    I  Y  H     P   +++K I
Sbjct: 141 HYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIAYYVNH-PLYTPVSDLQMK-I 200

Query: 196 GIGLFLIGIIGNFYHHYLLSKTR-KEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFI 255
           G G  L+  + NFY H LL   R   G   Y+IP+G LF+ V C +Y  EI ++ GF   
Sbjct: 201 GFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNIA 260

Query: 256 SQELYPLCFSIGTSIYLAGRSYATRKWYISK 284
           +Q       +I   ++LA  +     W + K
Sbjct: 261 TQ-------TIAGYVFLAVAALIMTNWALGK 282

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BLAST of Bhi07G000608 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 56.2 bits (134), Expect = 5.1e-08
Identity = 39/115 (33.91%), Postives = 54/115 (46.96%), Query Frame = 0


Query: 190 LKNIGIGLFLIGIIGNFYHHYLLSKTRK--EGETSYEIPKGGLFSFVICPHYLFEIIEFF 249
           L+ IG  +FL G I     H +L   R+       Y IP G  F  V CPH+L EI+ + 
Sbjct: 207 LQWIGGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYL 266

Query: 250 GFAFISQELYPLCFSIGTSIYLAGRSYA---TRKWYISKFENFPNHVKALLPFVF 300
           G   IS     +   +      A  +YA   T +WY+ KFEN+P    A+ P V+
Sbjct: 267 GL-LISSGGTDISIWLLFGFVAANLTYAAGETHRWYLQKFENYPASRHAIFPHVY 320

[Close]

BLAST of Bhi07G000608 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 53.9 bits (128), Expect = 2.6e-07
Identity = 38/117 (32.48%), Postives = 58/117 (49.57%), Query Frame = 0


Query: 190 LKNIGIGLFLIGIIGNFYHHYLLSKTRKEGETS--YEIPKGGLFSFVICPHYLFEIIEFF 249
           L+ IG  +FL G I     H +L   R+    +  Y IP G  F  V  PH+L EI+ + 
Sbjct: 230 LQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYA 289

Query: 250 GFAFISQ----ELYPLCFSIGTSI-YLAGRSYATRKWYISKFENFPNHVKALLPFVF 300
           G    S      ++ L   +  ++ Y AG    T +WY+ KFEN+P +  A+ P+V+
Sbjct: 290 GLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 343

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BLAST of Bhi07G000608 vs. ExPASy Swiss-Prot
Match: Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.4e-18
Identity = 43/107 (40.19%), Postives = 67/107 (62.62%), Query Frame = 0


Query: 193 IGIGLFLIGIIGNFYHHYLLSKTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFI 252
           IG GL+L G++ N +  ++L   RK G+T Y+IP+GGLF +V   +Y  EI+E+ G+A  
Sbjct: 157 IGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 216

Query: 253 SQELYPLCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 300
           S  +    F+  T  +L+GR+    +WY+ KFE +P   K L+PF+F
Sbjct: 217 SWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263

[Close]

BLAST of Bhi07G000608 vs. ExPASy Swiss-Prot
Match: P18405 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 4.1e-18
Identity = 42/107 (39.25%), Postives = 67/107 (62.62%), Query Frame = 0


Query: 193 IGIGLFLIGIIGNFYHHYLLSKTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFI 252
           IG GL+L G++ N +  ++L   RK G+T Y+IP+GGLF +V   +Y  EI+E+ G+A  
Sbjct: 153 IGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 212

Query: 253 SQELYPLCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 300
           S  +    F+  T  +L+GR+    +WY+ KFE +P   K ++PF+F
Sbjct: 213 SWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259

[Close]

BLAST of Bhi07G000608 vs. ExPASy Swiss-Prot
Match: P24008 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 PE=1 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 1.3e-16
Identity = 42/105 (40.00%), Postives = 64/105 (60.95%), Query Frame = 0


Query: 194 GIGLFLIGIIGNFYHHYLLSKTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFIS 253
           G  L+L+G++ N +  ++L   RK GET Y+IP+GGLF +V   +Y  E++E+ GFA  S
Sbjct: 154 GFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSAANYFGELVEWCGFALAS 213

Query: 254 QELYPLCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFV 299
             L  + F++ T   L  R+    +WY  KFE++P   K L+PFV
Sbjct: 214 WSLQGVVFALFTLSTLLTRAKQHHQWYHEKFEDYPKSRKILIPFV 258

[Close]

BLAST of Bhi07G000608 vs. ExPASy Swiss-Prot
Match: O18765 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa OX=9823 GN=SRD5A2 PE=2 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 1.3e-16
Identity = 41/108 (37.96%), Postives = 71/108 (65.74%), Query Frame = 0


Query: 192 NIGIGLFLIGIIGNFYHHYLLSKTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAF 251
           ++G+ LF++G+  N +  Y+L + RK GE  Y+IP+GGLF++V   ++L EIIE+ G+A 
Sbjct: 147 SLGVFLFILGMGINIHSDYILRQLRKPGEVIYKIPQGGLFTYVSGANFLGEIIEWIGYAL 206

Query: 252 ISQELYPLCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 300
            +  L  L F+  +  +L  R++   ++Y+  FE++P   KAL+PF+F
Sbjct: 207 ATWSLPALAFAFFSLCFLGLRAFHHHRFYVKMFEDYPKSRKALIPFIF 254

[Close]

BLAST of Bhi07G000608 vs. ExPASy Swiss-Prot
Match: Q55C17 (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn2 PE=3 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.7e-16
Identity = 67/242 (27.69%), Postives = 119/242 (49.17%), Query Frame = 0


Query: 62  AMAGISEINGKHL-QYSKFWNANTAPKLSSEVSMLSSKYGMLLAYTPAFLAGAASFWLFP 121
           A+   S++  KH+   S  +  +  P++S  +  +    G L  Y P F     S  ++ 
Sbjct: 61  ALGKDSDLVSKHVGADSTLYFKDLGPQISWSLVFICEYAGPLFVY-PIFY--FLSNLIYG 120

Query: 122 SADERILLLKSAL---TLHFFKRIFEVLFIHKYSSKMVLVTAIQISFSYFLSTVIMIYTQ 181
           +   +    K AL   +LH+ KRI+E +F+H++S   + +  +  + SY+     M+   
Sbjct: 121 TDSPKSFAQKVALVCYSLHYIKRIYETIFVHRFSHGTMPIFNLFKNCSYYWGCTAMVSYF 180

Query: 182 HLSQGLPEPPIELKNIGIGLFLIGIIGNFYHHYLLSKTRKEGETSYEIPKGGLFSFVICP 241
                  E PIE   +G+GL++IG + N+  H  L   R  G T  +IP+G LF FV CP
Sbjct: 181 VNHPLYTEAPIERVYLGLGLWIIGEVFNYICHIQLRNLRPAGSTERKIPRGLLFEFVSCP 240

Query: 242 HYLFEIIEFFGFAFISQELYPLCFSIGTSIYLAGRSYATRKWYISKF-ENFPNHVKALLP 299
           +Y  EI+ + GF+ ++Q L    F++  +  +   +    + Y  +F + +P   K L+P
Sbjct: 241 NYTVEILSWIGFSILTQTLTSWIFALMGAAQMWIWAVGKHRRYRKEFGDKYPKSRKILIP 299

[Close]

BLAST of Bhi07G000608 vs. ExPASy TrEMBL
Match: A0A1S3BFS0 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC103489360 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.8e-134
Identity = 241/274 (87.96%), Postives = 257/274 (93.80%), Query Frame = 0


Query: 26  FPIGISAAMDVLSAFLFPPPPSKFVNLMTVVSSTALAMAGISEINGKHLQYSKFWNANTA 85
           FPI +S AMD+LSAFLFPPPPS FVN+MTVVSST+LAMAG+SEI GKHLQYSKFWNAN+ 
Sbjct: 3   FPIWVSVAMDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSG 62

Query: 86  PKLSSEVSMLSSKYGMLLAYTPAFLAGAASFWLFPSADERILLLKSALTLHFFKRIFEVL 145
            KLS++ S LSSKYGMLL YTPAFLAGAASFWLFPS DER  LLKSALTLHFFKRIFEVL
Sbjct: 63  SKLSAQESKLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVL 122

Query: 146 FIHKYSSKMVLVTAIQISFSYFLSTVIMIYTQHLSQGLPEPPIELKNIGIGLFLIGIIGN 205
           FIHKYSSKMVL TAIQISFSYFLST IMIYTQHLSQGLPEPPI+LKNIG+GLFL GIIGN
Sbjct: 123 FIHKYSSKMVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLFLTGIIGN 182

Query: 206 FYHHYLLSKTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFISQELYPLCFSIGT 265
           FYHHYLLS+TRK+ ET+Y+IPKGGLFSFVICPHYLFEII +FGFAFISQELYPLCFSIGT
Sbjct: 183 FYHHYLLSQTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGT 242

Query: 266 SIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 300
           +IYLAGRSYATRKWYISKFENFPNHVKALLPFVF
Sbjct: 243 AIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 276

[Close]

BLAST of Bhi07G000608 vs. ExPASy TrEMBL
Match: A0A6J1GZA3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458588 PE=3 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 1.3e-128
Identity = 233/266 (87.59%), Postives = 248/266 (93.23%), Query Frame = 0


Query: 34  MDVLSAFLFPPPPSKFVNLMTVVSSTALAMAGISEINGKHLQYSKFWNANTAPKLSSEVS 93
           M +LSAFLFPPPPS FVN+MTVVSSTA+ MAGISEINGKHLQYSKFWNAN++PK  SE  
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 94  MLSSKYGMLLAYTPAFLAGAASFWLFPSADERILLLKSALTLHFFKRIFEVLFIHKYSSK 153
            LSSKYGMLLAYTPAFLAGAASFW FPS +ERIL+LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 154 MVLVTAIQISFSYFLSTVIMIYTQHLSQGLPEPPIELKNIGIGLFLIGIIGNFYHHYLLS 213
           MV  TAI+ISFSY LSTVIMIYTQHLSQGLPEPPI+LKNIGI LFLIGIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 214 KTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFISQELYPLCFSIGTSIYLAGRS 273
           KTRK GET Y+IPKGGLFS VICPHYLFEIIEFFGFAFISQELYPLCFS GT+IYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 274 YATRKWYISKFENFPNHVKALLPFVF 300
           YATRKWY+SKFENFP+HVKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of Bhi07G000608 vs. ExPASy TrEMBL
Match: A0A6J1J6R3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483043 PE=3 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.3e-128
Identity = 232/266 (87.22%), Postives = 248/266 (93.23%), Query Frame = 0


Query: 34  MDVLSAFLFPPPPSKFVNLMTVVSSTALAMAGISEINGKHLQYSKFWNANTAPKLSSEVS 93
           M +LSAFLFPPPPS FVN+MTVVSSTA+ MAGISEINGKHLQYSKFWNAN++PK  SE  
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 94  MLSSKYGMLLAYTPAFLAGAASFWLFPSADERILLLKSALTLHFFKRIFEVLFIHKYSSK 153
            LSSKYGMLLAYTPAFLAGAASFW FPS +ERIL+LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 154 MVLVTAIQISFSYFLSTVIMIYTQHLSQGLPEPPIELKNIGIGLFLIGIIGNFYHHYLLS 213
           MV  TAI+ISFSY LSTVIMIYTQHLSQG+PEPPI+LKNIGI LFLIGIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 214 KTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFISQELYPLCFSIGTSIYLAGRS 273
           KTRK GET Y+IPKGGLFS VICPHYLFEIIEFFGFAFISQELYPLCFS GT+IYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 274 YATRKWYISKFENFPNHVKALLPFVF 300
           YATRKWY+SKFENFP+HVKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of Bhi07G000608 vs. ExPASy TrEMBL
Match: A0A0A0KTC7 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G612880 PE=3 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 4.4e-124
Identity = 229/281 (81.49%), Postives = 248/281 (88.26%), Query Frame = 0


Query: 19  YTRLLKLFPIGISAAMDVLSAFLFPPPPSKFVNLMTVVSSTALAMAGISEINGKHLQYSK 78
           Y  LLKLFPI +SAAMDVLSAFLFPPPPS FVN+MTVVS+TALAMAG+SEINGKHLQYSK
Sbjct: 6   YPYLLKLFPIWVSAAMDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSK 65

Query: 79  FWNANTAPKLSSEVSMLSSKYGMLLAYTPAFLAGAASFWLFPSADERILLLKSALTLHFF 138
           FWNAN A KLS+E S +SSKYGMLLAYTPAFLAGAASFWLFPS DER             
Sbjct: 66  FWNANGASKLSAEESKVSSKYGMLLAYTPAFLAGAASFWLFPSGDER------------- 125

Query: 139 KRIFEVLFIHKYSSKMVLVTAIQISFSYFLSTVIMIYTQHLSQGLPEPPIELKNIGIGLF 198
                VLFIHKYSSKMVL TAIQIS SYF+ST IMIYTQHLSQGLPEPPI+LKNIG+G F
Sbjct: 126 -----VLFIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSF 185

Query: 199 LIGIIGNFYHHYLLSKTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFISQELYP 258
           LIGIIGNFYHHYLLS+TRK+G+T+Y+IPKGGLFSFV+CPHYLFEII +FGFAFISQELYP
Sbjct: 186 LIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYP 245

Query: 259 LCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 300
           LCFSIGT++YLAGRSYATRKWYISKFE FPN+VKALLPFVF
Sbjct: 246 LCFSIGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 268

[Close]

BLAST of Bhi07G000608 vs. ExPASy TrEMBL
Match: A0A6J1DAI6 (very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC111018692 PE=3 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 5.6e-119
Identity = 214/266 (80.45%), Postives = 237/266 (89.10%), Query Frame = 0


Query: 34  MDVLSAFLFPPPPSKFVNLMTVVSSTALAMAGISEINGKHLQYSKFWNANTAPKLSSEVS 93
           MD+ SAFLFPPPPS FVN+MTVVSSTA+AM+G SE+NGKHLQYSKFWNAN A K SSE S
Sbjct: 1   MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60

Query: 94  MLSSKYGMLLAYTPAFLAGAASFWLFPSADERILLLKSALTLHFFKRIFEVLFIHKYSSK 153
            +SSKYGMLLAYTPAFLAGAASFW FPS D RILLLKSALT HFFKRIFEVLF+HKYSSK
Sbjct: 61  KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK 120

Query: 154 MVLVTAIQISFSYFLSTVIMIYTQHLSQGLPEPPIELKNIGIGLFLIGIIGNFYHHYLLS 213
           MVL TA +IS SYFLST IMIYTQHLSQG P P I+LKNIGIG F++GIIGNFYHHYLLS
Sbjct: 121 MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS 180

Query: 214 KTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFISQELYPLCFSIGTSIYLAGRS 273
           +TR +GE  Y+IP GGLF  VICPHYLFEII +FGFAFISQ ++PLCF+ GT++YLAGRS
Sbjct: 181 RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS 240

Query: 274 YATRKWYISKFENFPNHVKALLPFVF 300
           YATRKWY+SKFE+FPNHVKALLPF+F
Sbjct: 241 YATRKWYVSKFEHFPNHVKALLPFLF 266

The following BLAST results are available for this feature:

BLAST of Bhi07G000608 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16010.1	5.2e-85	57.46	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	1.5e-12	27.75	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT3G55360.1	1.7e-11	31.13	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	5.1e-08	33.91	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	2.6e-07	32.48	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

BLAST of Bhi07G000608 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q28891	1.4e-18	40.19	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1...	[more]
P18405	4.1e-18	39.25	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 S...	[more]
P24008	1.3e-16	40.00	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 ...	[more]
O18765	1.3e-16	37.96	3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa OX=9823 GN=SRD5A2 PE=2 SV=...	[more]
Q55C17	1.7e-16	27.69	Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn...	[more]

BLAST of Bhi07G000608 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BFS0	2.8e-134	87.96	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A6J1GZA3	1.3e-128	87.59	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1J6R3	2.3e-128	87.22	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A0A0KTC7	4.4e-124	81.49	S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6128...	[more]
A0A6J1DAI6	5.6e-119	80.45	very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC11...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.120.1630		coord: 124..298 e-value: 5.3E-7 score: 31.5
None	No IPR available	PANTHER	PTHR10556:SF35	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN	coord: 39..299
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 189..299 e-value: 1.3E-28 score: 99.9
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 194..274 score: 25.393673
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 39..299

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi07M000608	Bhi07M000608	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors