Bhi05G001294 (gene) Wax gourd (B227) v1

Overview


Name	Bhi05G001294
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	sodium/potassium/calcium exchanger 1-like
Location	chr5: 50941929 .. 50942742 (+)
RNA-Seq Expression	Bhi05G001294
Synteny	Bhi05G001294

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCAATTCCACTCCAACCAATCTCCTTCAAAACACAAACCCACAACAACTTTTTTTTTCCTTGAAAAGTTTCAATTTTTCCACAAAAATTGAAACACCCAGTTGACCCAATTGGCCAAAATCATCAAATCTTGATCCAAAATTTCAATCCAATCCTTCAAAATTTGCAAATTTCCACAATGAAGTTAGTGATCTCAGCTTCTTTACTATCTGCAATGGTATTTTACTCTTCTCTCCTTCCTTTTCTCCACTCTTTGAACCTTTATATCTCTACAACAATCCACAAGAACTACATGTTCTTGCTCTGCAATGGCCTCCTTGTCTTCATCGTTCGAAATTCCGGTCTTATCGGCAACTCCAGCGACGAACAGAGTGGCAGAAATGAAGCTCTAGGGGCTGCAATGGCTGATGGGAACAGAAAAATTGAGAGCAAAGTTGAAATTGAAGAAGAAAGGGAAAATGGGTTGATTGCAAAAGAAGAAGAAAATGAAGAATTGATTACAAAGGATGAAGATGAAGAAGAAAGATTCAATTTTTATGAAGAAGAAGAAGAAGAAGAAGAAGAGGAAATTGACGAAGAATTGAATAAGAAATGTGAAGAATTTATTAGAAAAATGAAGGAAGGAATTAAATTTGAATCACAGCAAGTGTTTGTTTTGTAACTGAGAATTTATAAATGATTAAAAATTCTGACTTTGTAATTTTTTACTCTGTTCTTCACTTTCTCTTCAGTTGGATATAGAAATAATTTTTTTACCTTTCATTTTGGCATAATTAGGATAGAGAATTATGAAATTAAAATAAATAAAAAGCAC

mRNA sequence

CCCAATTCCACTCCAACCAATCTCCTTCAAAACACAAACCCACAACAACTTTTTTTTTCCTTGAAAAGTTTCAATTTTTCCACAAAAATTGAAACACCCAGTTGACCCAATTGGCCAAAATCATCAAATCTTGATCCAAAATTTCAATCCAATCCTTCAAAATTTGCAAATTTCCACAATGAAGTTAGTGATCTCAGCTTCTTTACTATCTGCAATGGTATTTTACTCTTCTCTCCTTCCTTTTCTCCACTCTTTGAACCTTTATATCTCTACAACAATCCACAAGAACTACATGTTCTTGCTCTGCAATGGCCTCCTTGTCTTCATCGTTCGAAATTCCGGTCTTATCGGCAACTCCAGCGACGAACAGAGTGGCAGAAATGAAGCTCTAGGGGCTGCAATGGCTGATGGGAACAGAAAAATTGAGAGCAAAGTTGAAATTGAAGAAGAAAGGGAAAATGGGTTGATTGCAAAAGAAGAAGAAAATGAAGAATTGATTACAAAGGATGAAGATGAAGAAGAAAGATTCAATTTTTATGAAGAAGAAGAAGAAGAAGAAGAAGAGGAAATTGACGAAGAATTGAATAAGAAATGTGAAGAATTTATTAGAAAAATGAAGGAAGGAATTAAATTTGAATCACAGCAAGTGTTTGTTTTGTAACTGAGAATTTATAAATGATTAAAAATTCTGACTTTGTAATTTTTTACTCTGTTCTTCACTTTCTCTTCAGTTGGATATAGAAATAATTTTTTTACCTTTCATTTTGGCATAATTAGGATAGAGAATTATGAAATTAAAATAAATAAAAAGCAC

Coding sequence (CDS)

ATGAAGTTAGTGATCTCAGCTTCTTTACTATCTGCAATGGTATTTTACTCTTCTCTCCTTCCTTTTCTCCACTCTTTGAACCTTTATATCTCTACAACAATCCACAAGAACTACATGTTCTTGCTCTGCAATGGCCTCCTTGTCTTCATCGTTCGAAATTCCGGTCTTATCGGCAACTCCAGCGACGAACAGAGTGGCAGAAATGAAGCTCTAGGGGCTGCAATGGCTGATGGGAACAGAAAAATTGAGAGCAAAGTTGAAATTGAAGAAGAAAGGGAAAATGGGTTGATTGCAAAAGAAGAAGAAAATGAAGAATTGATTACAAAGGATGAAGATGAAGAAGAAAGATTCAATTTTTATGAAGAAGAAGAAGAAGAAGAAGAAGAGGAAATTGACGAAGAATTGAATAAGAAATGTGAAGAATTTATTAGAAAAATGAAGGAAGGAATTAAATTTGAATCACAGCAAGTGTTTGTTTTGTAA

Protein sequence

MKLVISASLLSAMVFYSSLLPFLHSLNLYISTTIHKNYMFLLCNGLLVFIVRNSGLIGNSSDEQSGRNEALGAAMADGNRKIESKVEIEEERENGLIAKEEENEELITKDEDEEERFNFYEEEEEEEEEEIDEELNKKCEEFIRKMKEGIKFESQQVFVL

Homology

[Close]

BLAST of Bhi05G001294 vs. TAIR 10
Match: AT2G04495.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G04515.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 42.4 bits (98), Expect = 4.1e-04
Identity = 63/191 (32.98%), Postives = 97/191 (50.79%), Query Frame = 0


Query: 2   KLVISASLLSAMVFYSSLLPF----LHSLNLYISTTIHKNYMFLLCNGLLVFIVRNSGLI 61
           K++  AS+ S ++ YSSL+      LH L++Y S  + K YMFLLCNG++ FI+      
Sbjct: 18  KIIALASIFSFILSYSSLISSLQQRLHLLSMY-SNLVDKKYMFLLCNGIVGFIL--GSFH 77

Query: 62  GNSSDEQSGR-----NEALGAAMAD-GNRKIESKVEIEEERENGLIAKEEENEE-----L 121
           GNS     G+      +     + D    K++  V + EE+    ++KEEE EE     +
Sbjct: 78  GNSESLSQGKAINIIEQTKEVGIKDLKETKLKKVVALLEEKS---VSKEEEEEEEGGRVV 137

Query: 122 ITKDEDEEERFNFYEEEEE--------------EEEEEID------EELNKKCEEFIRKM 158
           + +  +EEE     E +EE               ++EEI+      E+LNKKCE+FIRKM
Sbjct: 138 LFRGIEEEEVSLVVEVDEEILVQDLAIIRVDDHHDDEEINDSLLSVEDLNKKCEDFIRKM 197

[Close]

BLAST of Bhi05G001294 vs. TAIR 10
Match: AT2G04515.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G04495.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 42.0 bits (97), Expect = 5.4e-04
Identity = 57/184 (30.98%), Postives = 93/184 (50.54%), Query Frame = 0


Query: 2   KLVISASLLSAMVFYSSLLPF----LHSLNLYISTTIHKNYMFLLCNGLLVFIVRNSGLI 61
           K++  AS+ S ++ YSSL+      LH L +Y S  + K YMFLLCNG++ FI+      
Sbjct: 18  KIIAFASIFSFILSYSSLISSLQQRLHLLAMY-SNLVDKKYMFLLCNGIVGFIL--GSFH 77

Query: 62  GNSSDEQSGR-----NEALGAAMADGNRKIESKVEIEEERENGLIAKEEENEELITKDED 121
           GNS     G+      +     + D      +KV    E  N  ++KEEE   +++++E+
Sbjct: 78  GNSDSLSHGKAINIVEQTKEVGIKDLKETKVNKVVALLEESN--VSKEEEGRVVLSREEE 137

Query: 122 EEERFNFYEEE-------------EEEEEEEID------EELNKKCEEFIRKMKEGIKFE 158
           EE      E+E             + ++++ I+      E+LNKKCE+FIRKMK  I++ 
Sbjct: 138 EEVALVVKEDEGILVQDLSITRLDDHDDDDAINDSLLSSEDLNKKCEDFIRKMKAEIRYG 196

[Close]

BLAST of Bhi05G001294 vs. ExPASy TrEMBL
Match: A0A0A0K349 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G032240 PE=4 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 3.0e-55
Identity = 136/161 (84.47%), Postives = 145/161 (90.06%), Query Frame = 0


Query: 1   MKLVISASLLSAMVFYSSLLPFLHSLNLYISTTIHKNYMFLLCNGLLVFIVRNSGLIGNS 60
           MKLVISASLLSAMVFYSSLLPFLHSLNLYIS+TIHKNYMFLLCNGLLVFIVRNSGLIGN+
Sbjct: 1   MKLVISASLLSAMVFYSSLLPFLHSLNLYISSTIHKNYMFLLCNGLLVFIVRNSGLIGNT 60

Query: 61  SDEQSGRNEALGAA-MADGNRKIESKVEIEEERENGLIAKEEENEELITKDEDEEERFNF 120
           SDE+S  NEALGAA MADGNRKIESKVEIEEER NGL+  E++ E LIT +E EEERF  
Sbjct: 61  SDERSSGNEALGAATMADGNRKIESKVEIEEERGNGLVVNEDKIENLITDNEVEEERFMS 120

Query: 121 YEEEEEEEEEEIDEELNKKCEEFIRKMKEGIKFESQQVFVL 161
           Y   EEEEEEEIDEELNKKCEEFIRKMK GIKFESQQ+F+L
Sbjct: 121 Y---EEEEEEEIDEELNKKCEEFIRKMKAGIKFESQQLFIL 158

[Close]

BLAST of Bhi05G001294 vs. ExPASy TrEMBL
Match: A0A1S3CG16 (gem-associated protein 2-like OS=Cucumis melo OX=3656 GN=LOC103500515 PE=4 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 6.6e-50
Identity = 127/159 (79.87%), Postives = 136/159 (85.53%), Query Frame = 0


Query: 13  MVFYSSLLPFLHSLNLYISTTIHKNYMFLLCNGLLVFIVRNSGLIGNSSDEQSGRNEALG 72
           MVFYSSLLPFLHSLNLYIS+TIHKNYMFLLCNGLLVFIVRNSGLIGN+ +EQS RNEALG
Sbjct: 1   MVFYSSLLPFLHSLNLYISSTIHKNYMFLLCNGLLVFIVRNSGLIGNTCNEQSSRNEALG 60

Query: 73  A-AMADGNRKIESK--VEIEEERENGLIAKEEENEELITKDEDEEERFNFY--------E 132
           A  MADGNRKIESK  VE+EEER NGL+  EE+ E LIT +EDEEERF  Y        E
Sbjct: 61  AVTMADGNRKIESKVEVEVEEERRNGLVVNEEKFENLITDNEDEEERFISYEEEEEEEEE 120

Query: 133 EEEEEEEEEIDEELNKKCEEFIRKMKEGIKFESQQVFVL 161
           EEEEEEEEEIDEELNKKCEEFIRKMK GIKFESQQ+F+L
Sbjct: 121 EEEEEEEEEIDEELNKKCEEFIRKMKAGIKFESQQLFIL 159

[Close]

BLAST of Bhi05G001294 vs. ExPASy TrEMBL
Match: A0A6J1EJY9 (uncharacterized protein LOC111435260 OS=Cucurbita moschata OX=3662 GN=LOC111435260 PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 3.1e-39
Identity = 113/164 (68.90%), Postives = 127/164 (77.44%), Query Frame = 0


Query: 1   MKLVISASLLSAMVFYSSLLPFLHSLNLYISTTIHKNYMFLLCNGLLVFIVRNSGLIGNS 60
           MKL+ISASLLSAMVFYSSLLPFLHS NLYIS T HKNYMFLLCNGLLVFIVRNSGLI  S
Sbjct: 1   MKLLISASLLSAMVFYSSLLPFLHSFNLYISATAHKNYMFLLCNGLLVFIVRNSGLISCS 60

Query: 61  SDEQSGRN----EALGAAMADGNRKIESKVEIEEERENGLIAKEEENEELITKDEDEEER 120
            D  SG +    EAL A+MADG+ KIESKVE  EER NGLI K+EE+E  I +D+D ++ 
Sbjct: 61  GDRHSGGDSRGAEALEASMADGSSKIESKVEDREERGNGLIQKDEESERSIVEDDDNDD- 120

Query: 121 FNFYEEEEEEEEEEIDEELNKKCEEFIRKMKEGIKFESQQVFVL 161
               +++EEE      EELNKKCEEFIRKMK GIK ESQQ+ VL
Sbjct: 121 ----DDDEEEVGALSKEELNKKCEEFIRKMKAGIKLESQQLLVL 159

[Close]

BLAST of Bhi05G001294 vs. ExPASy TrEMBL
Match: A0A6J1I0H9 (sodium/potassium/calcium exchanger 1-like OS=Cucurbita maxima OX=3661 GN=LOC111468328 PE=4 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 8.9e-39
Identity = 112/169 (66.27%), Postives = 129/169 (76.33%), Query Frame = 0


Query: 1   MKLVISASLLSAMVFYSSLLPFLHSLNLYISTTIHKNYMFLLCNGLLVFIVRNSGLIGNS 60
           MKLVISAS+LSAMVFYSSLLPFLHSLNLYIS TIHKNYMFLLCNGLLVFIVRNSGLI +S
Sbjct: 1   MKLVISASVLSAMVFYSSLLPFLHSLNLYISATIHKNYMFLLCNGLLVFIVRNSGLISSS 60

Query: 61  SDEQSGRN-----EALGAAMADGNRKIESKVEIEEERENGLIAKEEENEELITKDEDEEE 120
             EQ G +     EALGAAM D    IE KVE  EERENG IA+EE+  E +  +E++++
Sbjct: 61  GREQCGGHGSREAEALGAAMEDSTTIIERKVEDGEERENGFIAEEEQESERLVTEEEQQQ 120

Query: 121 RFNFYEEEEEEEEEEI----DEELNKKCEEFIRKMKEGIKFESQQVFVL 161
                +EEE+ E EE+    +EEL+KK EEFIRKMK GIK ESQQV +L
Sbjct: 121 EHEEEQEEEKAEAEEVGALSNEELDKKFEEFIRKMKAGIKLESQQVLIL 169

[Close]

BLAST of Bhi05G001294 vs. ExPASy TrEMBL
Match: A0A6J1C4P4 (ATP-dependent RNA helicase MAK5 OS=Momordica charantia OX=3673 GN=LOC111007892 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 1.3e-37
Identity = 109/159 (68.55%), Postives = 124/159 (77.99%), Query Frame = 0


Query: 1   MKLVISASLLSAMVFYSSLLPFLHSLNLYISTTIHKNYMFLLCNGLLVFIVRNSGLIGNS 60
           MK++ISASLLSAMV YSSLLPFLHSLNLYIS TIHKNY+FLLCNGLLVFIVRNSGLIG S
Sbjct: 1   MKILISASLLSAMVLYSSLLPFLHSLNLYISATIHKNYIFLLCNGLLVFIVRNSGLIGGS 60

Query: 61  SDEQSGRNEALGAAMADGNRKIESKVEIEEERENGLIAKEEENEELITKDEDEEERFNFY 120
            +EQ             G  K+ESKVE  EERENGL+A++EE+E+LIT+DE+ EE     
Sbjct: 61  GEEQG------------GGGKVESKVEDGEERENGLVAEQEESEKLITEDEEHEEE---- 120

Query: 121 EEEEEEEEEEIDEELNKKCEEFIRKMKEGIKFESQQVFV 160
           EEEEEE+    +EELN+KCEEFIRKMK  IKFE QQV V
Sbjct: 121 EEEEEEDGGLSNEELNQKCEEFIRKMKAEIKFELQQVLV 143

The following BLAST results are available for this feature:

BLAST of Bhi05G001294 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G04495.1	4.1e-04	32.98	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT2G04515.1	5.4e-04	30.98	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

BLAST of Bhi05G001294 vs. TAIR 10


Match Name	E-value	Identity	Description

BLAST of Bhi05G001294 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K349	3.0e-55	84.47	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G032240 PE=4 SV=1	[more]
A0A1S3CG16	6.6e-50	79.87	gem-associated protein 2-like OS=Cucumis melo OX=3656 GN=LOC103500515 PE=4 SV=1	[more]
A0A6J1EJY9	3.1e-39	68.90	uncharacterized protein LOC111435260 OS=Cucurbita moschata OX=3662 GN=LOC1114352...	[more]
A0A6J1I0H9	8.9e-39	66.27	sodium/potassium/calcium exchanger 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1C4P4	1.3e-37	68.55	ATP-dependent RNA helicase MAK5 OS=Momordica charantia OX=3673 GN=LOC111007892 P...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 96..141
None	No IPR available	PANTHER	PTHR34947:SF3	TRANSMEMBRANE PROTEIN	coord: 1..158
None	No IPR available	PANTHER	PTHR34947	TRANSMEMBRANE PROTEIN	coord: 1..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M001294	Bhi05M001294	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane