Bhi03G001829 (gene) Wax gourd (B227) v1

Overview
NameBhi03G001829
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationchr3: 50575303 .. 50577164 (-)
RNA-Seq ExpressionBhi03G001829
SyntenyBhi03G001829
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAAACATGGTTACTCATATTATGGTACTTCGACATCCTCTTCAAGCATTTATTTTATTTGGAAATATGTTTTTCGAGATAGTTTTCTCCCCCTCATCATGATAGAGTTTGAAGGAGTAGTTGATAGTTGGTTGTATGCTGTTTTTTTTTTCCCACTAAAAAATGAAAACGAAAAACAAAATATTGTCAAAATCTCTCAACAAAAATCTATTGAGTAAAAAAATAATAAATAAAAAAAATGCCCTAAAATTGTGGTCATGGGTCATTTACATTTTACATAAGCAATGACGCATCACCATCATCTTCAACTTACTGTAACGTTGCTTTCCTCTTCCTCCTCCATTAAAATGGCGGTTCTCGTTCTTCGCTCACACTAATTCCCCACCGCCATTTTCAAACACCCTTCTCAAATCAACCCTTTCATTTTCCGATTAAACCCATGGCCGCCGTCTCCGCCGCTCTGCCGCTCGACGGCCGAACCGCAATCGTCACCGGCGCATCCCGTGGCATTGGCCGCGCCATCGCCGTCCACCTCCACTTCCTCGGCGCAAACCTCGTCATCAACTACGCTTCCAACTCTTCACAAGCCGATCTGCTCGCATCGGAGCTCAACCAGTCATCGGCGCCGCTCCGGCGAGCCGTCGTTGTCCAGGCCGACGTGTCTGATCCAGATCAGGTAAAGCGGTTGTTCGATTCGGCGGAAAAGGAGTTTGGATCTGAGATTCACATCGTCGTGAACTCCGCTGGGATTCTTGATTCGAAGTACCCGAGTCTGGCCGGAACCGCCGTGGAGGATTGGGATGAGGCGTTTCGAGTGAATTGCAGAGGCGCGTTTTTGGTCTGCAAAGAGGCGTCGAATCGGCTCAGGCGCGGCGGTGGAGGGCGGGTTGTGTTGCTTACGACGTCGATCGTTGGGTCTCTGCCGCCGGGATACGGTGCTTATGCGGCGTCGAAAGCGGCGGTGGAGGCGATGGCGAAAATTGCGGCGAAGGAGCTCAAGGGGACGGAAATCACGGTTAACTGCGTGGCGCCAGGACCGGTGGCGACGGAGCTTTTTTACGCCGGGAAATCAGAGGAGACGGTGGCGAAGATGGCGGAGGCCTGCCCTCTAGGCCGGCTTGGGCAGCCCGACGACGTGGCGAAAGTGGTTGGATTTTTGGTTACTGATGCCGGTGGATGGGTCAACGGGCAGGTCGTTCGGGTCAATGGAGGGCTTGTAATTTGATTTTGGAAGGGAAGTAATTGAAACGCTATAGGAGTGAATTTTTTTCCCCCTTTTCTAAGAAAGTATTGGAATTAAAATTAATTTGAGTTACCATATTTTACGAATCAGAATGGGATTCCGCAATTTTTTATTCTCCTTCTTGCAAAGTTAACACTCTCCTTGAGTTCGAGTTGAGGGTAAATTTGTCAAAAAAAATATCCGCCATATCTAAACTTTCAATTTCATATTTAGTATGTCTAAAATAAGTTCAAAATTATTTAAAAAAATTAATTAATTCATCTATTAAATATAAAATTGAGTTAATATTTCTCTAAACTTATCTATAAATATCCTACTTAAAACTATTTAAAAAAATTTATGTACCAAATAAATACTGATTTCAAAATTTAATAAATAAATCACTAATTTAACTTATATTATAATTTTCAATTAGCACGTCATAGATAAAAACATATATTAATGGATTTAACTTTTTTTTTCATCAAAATACATTTTTAAATATATACATTTCAAATATTTTAATTTTTAAACAAAGTAATTAATTTAAAATCATTTTTAAATTTTCAAATGATAAGATTTAAAATAGATTCTTTTGACTATCTTATCCTAAATGAAATGAAGTGTAAAATTAAATTT

mRNA sequence

GGAAAACATGGTTACTCATATTATGGTACTTCGACATCCTCTTCAAGCATTTATTTTATTTGGAAATATGTTTTTCGAGATAGTTTTCTCCCCCTCATCATGATAGAGTTTGAAGGAGTAGTTGATAGTTGGTTGTATGCTGTTTTTTTTTTCCCACTAAAAAATGAAAACGAAAAACAAAATATTGTCAAAATCTCTCAACAAAAATCTATTGAGTAAAAAAATAATAAATAAAAAAAATGCCCTAAAATTGTGGTCATGGGTCATTTACATTTTACATAAGCAATGACGCATCACCATCATCTTCAACTTACTGTAACGTTGCTTTCCTCTTCCTCCTCCATTAAAATGGCGGTTCTCGTTCTTCGCTCACACTAATTCCCCACCGCCATTTTCAAACACCCTTCTCAAATCAACCCTTTCATTTTCCGATTAAACCCATGGCCGCCGTCTCCGCCGCTCTGCCGCTCGACGGCCGAACCGCAATCGTCACCGGCGCATCCCGTGGCATTGGCCGCGCCATCGCCGTCCACCTCCACTTCCTCGGCGCAAACCTCGTCATCAACTACGCTTCCAACTCTTCACAAGCCGATCTGCTCGCATCGGAGCTCAACCAGTCATCGGCGCCGCTCCGGCGAGCCGTCGTTGTCCAGGCCGACGTGTCTGATCCAGATCAGGTAAAGCGGTTGTTCGATTCGGCGGAAAAGGAGTTTGGATCTGAGATTCACATCGTCGTGAACTCCGCTGGGATTCTTGATTCGAAGTACCCGAGTCTGGCCGGAACCGCCGTGGAGGATTGGGATGAGGCGTTTCGAGTGAATTGCAGAGGCGCGTTTTTGGTCTGCAAAGAGGCGTCGAATCGGCTCAGGCGCGGCGGTGGAGGGCGGGTTGTGTTGCTTACGACGTCGATCGTTGGGTCTCTGCCGCCGGGATACGGTGCTTATGCGGCGTCGAAAGCGGCGGTGGAGGCGATGGCGAAAATTGCGGCGAAGGAGCTCAAGGGGACGGAAATCACGGTTAACTGCGTGGCGCCAGGACCGGTGGCGACGGAGCTTTTTTACGCCGGGAAATCAGAGGAGACGGTGGCGAAGATGGCGGAGGCCTGCCCTCTAGGCCGGCTTGGGCAGCCCGACGACGTGGCGAAAGTGGTTGGATTTTTGGTTACTGATGCCGGTGGATGGGTCAACGGGCAGGTCGTTCGGGTCAATGGAGGGCTTGTAATTTGATTTTGGAAGGGAAGTAATTGAAACGCTATAGGAGTGAATTTTTTTCCCCCTTTTCTAAGAAAGTATTGGAATTAAAATTAATTTGAGTTACCATATTTTACGAATCAGAATGGGATTCCGCAATTTTTTATTCTCCTTCTTGCAAAGTTAACACTCTCCTTGAGTTCGAGTTGAGGGTAAATTTGTCAAAAAAAATATCCGCCATATCTAAACTTTCAATTTCATATTTAGTATGTCTAAAATAAGTTCAAAATTATTTAAAAAAATTAATTAATTCATCTATTAAATATAAAATTGAGTTAATATTTCTCTAAACTTATCTATAAATATCCTACTTAAAACTATTTAAAAAAATTTATGTACCAAATAAATACTGATTTCAAAATTTAATAAATAAATCACTAATTTAACTTATATTATAATTTTCAATTAGCACGTCATAGATAAAAACATATATTAATGGATTTAACTTTTTTTTTCATCAAAATACATTTTTAAATATATACATTTCAAATATTTTAATTTTTAAACAAAGTAATTAATTTAAAATCATTTTTAAATTTTCAAATGATAAGATTTAAAATAGATTCTTTTGACTATCTTATCCTAAATGAAATGAAGTGTAAAATTAAATTT

Coding sequence (CDS)

ATGGCCGCCGTCTCCGCCGCTCTGCCGCTCGACGGCCGAACCGCAATCGTCACCGGCGCATCCCGTGGCATTGGCCGCGCCATCGCCGTCCACCTCCACTTCCTCGGCGCAAACCTCGTCATCAACTACGCTTCCAACTCTTCACAAGCCGATCTGCTCGCATCGGAGCTCAACCAGTCATCGGCGCCGCTCCGGCGAGCCGTCGTTGTCCAGGCCGACGTGTCTGATCCAGATCAGGTAAAGCGGTTGTTCGATTCGGCGGAAAAGGAGTTTGGATCTGAGATTCACATCGTCGTGAACTCCGCTGGGATTCTTGATTCGAAGTACCCGAGTCTGGCCGGAACCGCCGTGGAGGATTGGGATGAGGCGTTTCGAGTGAATTGCAGAGGCGCGTTTTTGGTCTGCAAAGAGGCGTCGAATCGGCTCAGGCGCGGCGGTGGAGGGCGGGTTGTGTTGCTTACGACGTCGATCGTTGGGTCTCTGCCGCCGGGATACGGTGCTTATGCGGCGTCGAAAGCGGCGGTGGAGGCGATGGCGAAAATTGCGGCGAAGGAGCTCAAGGGGACGGAAATCACGGTTAACTGCGTGGCGCCAGGACCGGTGGCGACGGAGCTTTTTTACGCCGGGAAATCAGAGGAGACGGTGGCGAAGATGGCGGAGGCCTGCCCTCTAGGCCGGCTTGGGCAGCCCGACGACGTGGCGAAAGTGGTTGGATTTTTGGTTACTGATGCCGGTGGATGGGTCAACGGGCAGGTCGTTCGGGTCAATGGAGGGCTTGTAATTTGA

Protein sequence

MAAVSAALPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQSSAPLRRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDWDEAFRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMAKIAAKELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGFLVTDAGGWVNGQVVRVNGGLVI
Homology
BLAST of Bhi03G001829 vs. TAIR 10
Match: AT4G13180.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 344.0 bits (881), Expect = 1.1e-94
Identity = 165/257 (64.20%), Postives = 209/257 (81.32%), Query Frame = 0

Query: 5   SAALPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQSSAPL 64
           S++LPL GR AIVTGA+RG+GR IA+HLH LGA + INY S+SS+A+LL SELN SS  L
Sbjct: 8   SSSLPLAGRVAIVTGATRGMGREIAIHLHSLGARVTINYVSSSSKAELLVSELNDSS-QL 67

Query: 65  RRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDWDEAF 124
           + A+ V+ADVSDPDQ+  LFD  E+EFGS++HIVVN AG+LD KYPSL+ T +ED+D  F
Sbjct: 68  KSAIAVKADVSDPDQINNLFDQTEQEFGSKVHIVVNCAGVLDPKYPSLSETTLEDFDNTF 127

Query: 125 RVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMAKIAAK 184
            +N RG+FL CKEA+ R+ RGGGGR+++++TS+VG L PGYG YAASKAAVE M K+ AK
Sbjct: 128 TINTRGSFLCCKEAAKRVMRGGGGRIIMMSTSMVGGLAPGYGVYAASKAAVETMVKVLAK 187

Query: 185 ELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGFLVTDA 244
           ELKG+ IT NCVAPGPVATE+FYAGKS+ETV  +A ACP+GR+G+  D+ ++VGFL  D 
Sbjct: 188 ELKGSRITANCVAPGPVATEMFYAGKSDETVKMLAGACPMGRIGESKDITEIVGFLAGDG 247

Query: 245 GGWVNGQVVRVNGGLVI 262
           G W+NGQV+R NGG V+
Sbjct: 248 GEWINGQVIRANGGFVV 263

BLAST of Bhi03G001829 vs. TAIR 10
Match: AT3G03980.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 307.0 bits (785), Expect = 1.5e-83
Identity = 160/261 (61.30%), Postives = 199/261 (76.25%), Query Frame = 0

Query: 8   LPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQSSAPLR-- 67
           LPL GR AIVTG+SRGIGRAIA+HL  LGA +VINY S ++ A+ +ASE+N    P+R  
Sbjct: 12  LPLAGRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERVASEIN--DFPVREE 71

Query: 68  ------RAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVED 127
                 RA+VVQA+VS+P QVK +FD+AE  F + +HI+VNSAGILD KYP++A T+VED
Sbjct: 72  ITGKGPRAIVVQANVSEPSQVKSMFDAAESAFEAPVHILVNSAGILDPKYPTIADTSVED 131

Query: 128 WDEAFRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMA 187
           +D  F VN +GAFL  KEA+NRL++GGGGR++LLT+S   SL PG+GAYAASKAAVE M 
Sbjct: 132 FDHTFSVNTKGAFLCSKEAANRLKQGGGGRIILLTSSQTRSLKPGFGAYAASKAAVETMV 191

Query: 188 KIAAKELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGF 247
           KI AKELKGT IT NCVAPGP+ATE+F+ GK+ E V K+A   P GR+G+  DV  +VGF
Sbjct: 192 KILAKELKGTGITANCVAPGPIATEMFFDGKTPELVEKIAAESPFGRVGEAKDVVPLVGF 251

Query: 248 LVTDAGGWVNGQVVRVNGGLV 261
           L  D G WVNGQ++ VNGG V
Sbjct: 252 LAGDGGEWVNGQIIPVNGGYV 270

BLAST of Bhi03G001829 vs. TAIR 10
Match: AT5G18210.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 288.9 bits (738), Expect = 4.1e-78
Identity = 148/243 (60.91%), Postives = 187/243 (76.95%), Query Frame = 0

Query: 5   SAALPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQS--SA 64
           S+   L GR AIVTG+SRGIGRAIA+HL  LGA +VINY + S++AD +A+E+N S  + 
Sbjct: 3   SSVSSLAGRVAIVTGSSRGIGRAIAIHLAELGAKIVINYTTRSTEADQVAAEINSSAGTV 62

Query: 65  PLRRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDWDE 124
           P   AVV  AD+S+P Q+K LFD+AEK F S +HI+VNSAGIL+  YP++A T +E++D 
Sbjct: 63  PQPIAVVFLADISEPSQIKSLFDAAEKAFNSPVHILVNSAGILNPNYPTIANTPIEEFDR 122

Query: 125 AFRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMAKIA 184
            F+VN RG+FL CKEA+ RL+RGGGGR++LLT+S+  +L PG GAY ASKAAVEAM KI 
Sbjct: 123 IFKVNTRGSFLCCKEAAKRLKRGGGGRIILLTSSLTEALIPGQGAYTASKAAVEAMVKIL 182

Query: 185 AKELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGFLVT 244
           AKELKG  IT NCV+PGPVATE+F+ GKSEETV  + E  P GRLG+  D+A VVGFL +
Sbjct: 183 AKELKGLGITANCVSPGPVATEMFFDGKSEETVMNIIERSPFGRLGETKDIASVVGFLAS 242

Query: 245 DAG 246
           D G
Sbjct: 243 DGG 245

BLAST of Bhi03G001829 vs. TAIR 10
Match: AT3G04000.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 274.6 bits (701), Expect = 8.0e-74
Identity = 147/272 (54.04%), Postives = 196/272 (72.06%), Query Frame = 0

Query: 1   MAAVSAA----LPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASE 60
           MAA S+     L L GR AIVTG+SRGIGRAIA+HL  LGA +V+NY+++  +A+ +A+ 
Sbjct: 1   MAAASSVSSPPLCLAGRVAIVTGSSRGIGRAIAIHLAELGARVVVNYSTSPVEAEKVATA 60

Query: 61  LN-------QSSAPLRRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKY 120
           +        + +    R +VV+AD+S+P QVK LFD AE+ F S +HI+VNSA I D  +
Sbjct: 61  ITTNCSKDAEVAGKSPRVIVVKADISEPSQVKSLFDEAERVFESPVHILVNSAAIADPNH 120

Query: 121 PSLAGTAVEDWDEAFRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYA 180
            +++  +VE +D    VN RGAF+  +EA+NRL+RGGGGR++LL+TS+V +L   YG+Y 
Sbjct: 121 STISDMSVELFDRIISVNTRGAFICAREAANRLKRGGGGRIILLSTSLVQTLNTNYGSYT 180

Query: 181 ASKAAVEAMAKIAAKELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQ 240
           ASKAAVEAMAKI AKELKGTEITVNCV+PGPVATE+FY G S E V K+      GR+G+
Sbjct: 181 ASKAAVEAMAKILAKELKGTEITVNCVSPGPVATEMFYTGLSNEIVEKVKSQNLFGRIGE 240

Query: 241 PDDVAKVVGFLVTDAGGWVNGQVVRVNGGLVI 262
             D+A VVGFL +DAG W+NGQV+  NGG ++
Sbjct: 241 TKDIAPVVGFLASDAGEWINGQVIMANGGCLL 272

BLAST of Bhi03G001829 vs. TAIR 10
Match: AT1G24360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 2.8e-26
Identity = 82/254 (32.28%), Postives = 141/254 (55.51%), Query Frame = 0

Query: 10  LDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQSSAPLRRAVV 69
           ++    ++TGASRGIG+AIA+ L   G  +++NYA ++ +A+ +A ++ +      +A+ 
Sbjct: 74  VESPVVVITGASRGIGKAIALALGKAGCKVLVNYARSAKEAEEVAKQIEEYGG---QAIT 133

Query: 70  VQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDWDEAFRVNCR 129
              DVS    V  +  +A  ++G+ I +VVN+AGI  ++   L       WDE   +N  
Sbjct: 134 FGGDVSKATDVDAMMKTALDKWGT-IDVVVNNAGI--TRDTLLIRMKQSQWDEVIALNLT 193

Query: 130 GAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPP-GYGAYAASKAAVEAMAKIAAKELKG 189
           G FL  + A   + +   GR++ + +S+VG +   G   YAA+K  V + +K AA+E   
Sbjct: 194 GVFLCTQAAVKIMMKKKRGRIINI-SSVVGLIGNIGQANYAAAKGGVISFSKTAAREGAS 253

Query: 190 TEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGFL-VTDAGGW 249
             I VN V PG +A+++  A   E+   K+    PLGR G+ ++VA +V FL ++ A  +
Sbjct: 254 RNINVNVVCPGFIASDM-TAELGEDMEKKILGTIPLGRYGKAEEVAGLVEFLALSPAASY 313

Query: 250 VNGQVVRVNGGLVI 262
           + GQ   ++GG+ I
Sbjct: 314 ITGQAFTIDGGIAI 319

BLAST of Bhi03G001829 vs. ExPASy Swiss-Prot
Match: Q9SQR4 (NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g03980 PE=3 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.1e-82
Identity = 160/261 (61.30%), Postives = 199/261 (76.25%), Query Frame = 0

Query: 8   LPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQSSAPLR-- 67
           LPL GR AIVTG+SRGIGRAIA+HL  LGA +VINY S ++ A+ +ASE+N    P+R  
Sbjct: 12  LPLAGRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERVASEIN--DFPVREE 71

Query: 68  ------RAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVED 127
                 RA+VVQA+VS+P QVK +FD+AE  F + +HI+VNSAGILD KYP++A T+VED
Sbjct: 72  ITGKGPRAIVVQANVSEPSQVKSMFDAAESAFEAPVHILVNSAGILDPKYPTIADTSVED 131

Query: 128 WDEAFRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMA 187
           +D  F VN +GAFL  KEA+NRL++GGGGR++LLT+S   SL PG+GAYAASKAAVE M 
Sbjct: 132 FDHTFSVNTKGAFLCSKEAANRLKQGGGGRIILLTSSQTRSLKPGFGAYAASKAAVETMV 191

Query: 188 KIAAKELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGF 247
           KI AKELKGT IT NCVAPGP+ATE+F+ GK+ E V K+A   P GR+G+  DV  +VGF
Sbjct: 192 KILAKELKGTGITANCVAPGPIATEMFFDGKTPELVEKIAAESPFGRVGEAKDVVPLVGF 251

Query: 248 LVTDAGGWVNGQVVRVNGGLV 261
           L  D G WVNGQ++ VNGG V
Sbjct: 252 LAGDGGEWVNGQIIPVNGGYV 270

BLAST of Bhi03G001829 vs. ExPASy Swiss-Prot
Match: Q08632 (Short-chain type dehydrogenase/reductase OS=Picea abies OX=3329 PE=2 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 3.9e-73
Identity = 147/256 (57.42%), Postives = 192/256 (75.00%), Query Frame = 0

Query: 8   LPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQ---SSAPL 67
           LPL GR AIVTGASRGIGR IA+++   GA +VI+Y+SN   A+ +AS +N    SS   
Sbjct: 17  LPLGGRVAIVTGASRGIGREIALNMAEKGAKVVIHYSSNQHAAEEVASIINNKSPSSGDG 76

Query: 68  RRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDWDEAF 127
            RA+V +ADV++P QV +LFD+AE  FG  +HIVVN+AG+ DSKYP+LA T+ E+WD  F
Sbjct: 77  VRAIVCKADVAEPSQVAQLFDTAEHAFG-PLHIVVNNAGVTDSKYPTLAQTSDEEWDRIF 136

Query: 128 RVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMAKIAAK 187
           +VNC+GAFL  +EA+ R+ RGGGGR++ +++S+V    P YGAY ASKAAVE M +I A+
Sbjct: 137 QVNCKGAFLCSREAAKRVVRGGGGRIINISSSLVAMPIPRYGAYTASKAAVEMMTRILAQ 196

Query: 188 ELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGFLVTDA 247
           EL+GT+IT NCVAPGPVAT++F+AGKSE  V    ++ P  RLG+ +DVA +V FL +D 
Sbjct: 197 ELRGTQITANCVAPGPVATDMFFAGKSEAAVEAGVKSNPFERLGKVEDVAPLVAFLASDE 256

Query: 248 GGWVNGQVVRVNGGLV 261
           G WVN QVVRVNGG V
Sbjct: 257 GEWVNAQVVRVNGGQV 271

BLAST of Bhi03G001829 vs. ExPASy Swiss-Prot
Match: Q9SQR2 (NADPH-dependent aldehyde reductase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ChlADR2 PE=1 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 1.1e-72
Identity = 147/272 (54.04%), Postives = 196/272 (72.06%), Query Frame = 0

Query: 1   MAAVSAA----LPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASE 60
           MAA S+     L L GR AIVTG+SRGIGRAIA+HL  LGA +V+NY+++  +A+ +A+ 
Sbjct: 1   MAAASSVSSPPLCLAGRVAIVTGSSRGIGRAIAIHLAELGARVVVNYSTSPVEAEKVATA 60

Query: 61  LN-------QSSAPLRRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKY 120
           +        + +    R +VV+AD+S+P QVK LFD AE+ F S +HI+VNSA I D  +
Sbjct: 61  ITTNCSKDAEVAGKSPRVIVVKADISEPSQVKSLFDEAERVFESPVHILVNSAAIADPNH 120

Query: 121 PSLAGTAVEDWDEAFRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYA 180
            +++  +VE +D    VN RGAF+  +EA+NRL+RGGGGR++LL+TS+V +L   YG+Y 
Sbjct: 121 STISDMSVELFDRIISVNTRGAFICAREAANRLKRGGGGRIILLSTSLVQTLNTNYGSYT 180

Query: 181 ASKAAVEAMAKIAAKELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQ 240
           ASKAAVEAMAKI AKELKGTEITVNCV+PGPVATE+FY G S E V K+      GR+G+
Sbjct: 181 ASKAAVEAMAKILAKELKGTEITVNCVSPGPVATEMFYTGLSNEIVEKVKSQNLFGRIGE 240

Query: 241 PDDVAKVVGFLVTDAGGWVNGQVVRVNGGLVI 262
             D+A VVGFL +DAG W+NGQV+  NGG ++
Sbjct: 241 TKDIAPVVGFLASDAGEWINGQVIMANGGCLL 272

BLAST of Bhi03G001829 vs. ExPASy Swiss-Prot
Match: P0CU75 (Short chain dehydrogenase claC OS=Passalora fulva OX=5499 GN=claC PE=1 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.3e-39
Identity = 99/261 (37.93%), Postives = 153/261 (58.62%), Query Frame = 0

Query: 10  LDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQSSAPLRRAVV 69
           LDGR A+VTG+ RGIG AIAVHL  LGAN+V+NYA+++  A  +  ++  + +    A+ 
Sbjct: 13  LDGRVAVVTGSGRGIGAAIAVHLGRLGANIVVNYANSALDAQKVVDQIKGAGS---EAIA 72

Query: 70  VQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDWDEAFRVNCR 129
           ++AD+ D  Q+ RLFD A   FG  + I V+++G++   +  L     E++D  F +N R
Sbjct: 73  IKADIRDVSQIMRLFDEAVAHFG-HVDIAVSNSGVV--SFGHLKDVTEEEFDRVFSLNTR 132

Query: 130 GAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLP-PGYGAYAASKAAVEAMAKIAAKELKG 189
           G F V +EA   L    GGR+++ +++       P +  Y+ SK AV++  +I +K+   
Sbjct: 133 GQFFVAREAYRHLSE--GGRIIMTSSNTSKDFSVPRHSLYSGSKGAVDSFVRIFSKDCGD 192

Query: 190 TEITVNCVAPGPVATELF------YAGKSEETVAKM-----AEACPLGRLGQPDDVAKVV 249
            +ITVN VAPG   T++F      Y    E+  A+      A A PL R G P+D+A VV
Sbjct: 193 KKITVNGVAPGGTVTDMFHDVSHHYIPDGEKYTAEQRQQMAAFASPLHRNGFPEDIANVV 252

Query: 250 GFLVTDAGGWVNGQVVRVNGG 259
           GFL +  G W+NG+V+ ++GG
Sbjct: 253 GFLASKEGEWINGKVINLDGG 265

BLAST of Bhi03G001829 vs. ExPASy Swiss-Prot
Match: Q00791 (Versicolorin reductase OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=stcU PE=3 SV=2)

HSP 1 Score: 163.7 bits (413), Expect = 2.8e-39
Identity = 101/262 (38.55%), Postives = 151/262 (57.63%), Query Frame = 0

Query: 10  LDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQSSAPLRRAVV 69
           LDG+ A+VTGA RGIG AIAV L   GA +V+NYA++   A+ +  E+  + +    A+ 
Sbjct: 9   LDGKVALVTGAGRGIGAAIAVALGQRGAKVVVNYANSREAAEKVVDEIKSNGSD---AIS 68

Query: 70  VQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDWDEAFRVNCR 129
           +QADV DPD V +L D A + FG  + IV ++AGI+   +  +     +++D  FRVN R
Sbjct: 69  IQADVGDPDAVTKLMDQAVEHFG-YLDIVSSNAGIV--SFGHVKDVTPDEFDRVFRVNTR 128

Query: 130 GAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLP--PGYGAYAASKAAVEAMAKIAAKELK 189
           G F V +EA   LR GG    ++LT+S   S+   P +  Y+ SK A++   +  A +  
Sbjct: 129 GQFFVAREAYRHLREGGR---IILTSSNTASVKGVPRHAVYSGSKGAIDTFVRCLAIDCG 188

Query: 190 GTEITVNCVAPGPVATELF------YAGKSEETVAKMAEAC-----PLGRLGQPDDVAKV 249
             +ITVN VAPG + T++F      Y    E    +  + C     PL R+G P DVA+V
Sbjct: 189 DKKITVNAVAPGAIKTDMFLSVSREYIPNGETFTDEQVDECAAWLSPLNRVGLPVDVARV 248

Query: 250 VGFLVTDAGGWVNGQVVRVNGG 259
           V FL +DA  W++G+++ V+GG
Sbjct: 249 VSFLASDAAEWISGKIIGVDGG 261

BLAST of Bhi03G001829 vs. NCBI nr
Match: XP_038883178.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic [Benincasa hispida])

HSP 1 Score: 498.4 bits (1282), Expect = 3.7e-137
Identity = 261/261 (100.00%), Postives = 261/261 (100.00%), Query Frame = 0

Query: 1   MAAVSAALPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQS 60
           MAAVSAALPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQS
Sbjct: 1   MAAVSAALPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQS 60

Query: 61  SAPLRRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDW 120
           SAPLRRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDW
Sbjct: 61  SAPLRRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDW 120

Query: 121 DEAFRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMAK 180
           DEAFRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMAK
Sbjct: 121 DEAFRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMAK 180

Query: 181 IAAKELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGFL 240
           IAAKELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGFL
Sbjct: 181 IAAKELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGFL 240

Query: 241 VTDAGGWVNGQVVRVNGGLVI 262
           VTDAGGWVNGQVVRVNGGLVI
Sbjct: 241 VTDAGGWVNGQVVRVNGGLVI 261

BLAST of Bhi03G001829 vs. NCBI nr
Match: XP_004147531.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic [Cucumis sativus] >KGN53946.1 hypothetical protein Csa_021571 [Cucumis sativus])

HSP 1 Score: 455.3 bits (1170), Expect = 3.6e-124
Identity = 233/258 (90.31%), Postives = 246/258 (95.35%), Query Frame = 0

Query: 4   VSAALPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQSSAP 63
           ++ ALPLDGRTAIVTGASRGIGRAIA+HLH LGANLV+NYASNS+QADLLASELNQSSAP
Sbjct: 1   MATALPLDGRTAIVTGASRGIGRAIAIHLHSLGANLVLNYASNSTQADLLASELNQSSAP 60

Query: 64  LRRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDWDEA 123
           LRRAV VQADVSDPD VKRLFDSAEKEFGSEIHI+VNSAGILDSKYPSL  T VEDWDE 
Sbjct: 61  LRRAVAVQADVSDPDHVKRLFDSAEKEFGSEIHILVNSAGILDSKYPSLVETTVEDWDET 120

Query: 124 FRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMAKIAA 183
           FRVNCRGAFLVCKEA NR++RGGGGR+VL+TTSIV SLPPGYGAYAASKAAVEAMAKIAA
Sbjct: 121 FRVNCRGAFLVCKEAENRIKRGGGGRIVLITTSIVLSLPPGYGAYAASKAAVEAMAKIAA 180

Query: 184 KELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGFLVTD 243
           KELKGT ITVNCVAPGPVATELFYAGKSEETVA+MAEACP+GRLGQPDD+AKVVGFLVTD
Sbjct: 181 KELKGTGITVNCVAPGPVATELFYAGKSEETVARMAEACPMGRLGQPDDLAKVVGFLVTD 240

Query: 244 AGGWVNGQVVRVNGGLVI 262
           AG WVNGQVVRVNGGLVI
Sbjct: 241 AGEWVNGQVVRVNGGLVI 258

BLAST of Bhi03G001829 vs. NCBI nr
Match: XP_008441973.1 (PREDICTED: short-chain type dehydrogenase/reductase [Cucumis melo])

HSP 1 Score: 453.0 bits (1164), Expect = 1.8e-123
Identity = 232/261 (88.89%), Postives = 248/261 (95.02%), Query Frame = 0

Query: 1   MAAVSAALPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQS 60
           M+ V+A LPL GRTAIVTGASRGIGRAIA+HLH LGANLV+NYASNS+QADLLAS+LNQS
Sbjct: 1   MSTVAAPLPLHGRTAIVTGASRGIGRAIAIHLHSLGANLVLNYASNSTQADLLASDLNQS 60

Query: 61  SAPLRRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDW 120
           SAPLRRAV VQADVSDPDQVKRLFDSAEKEFGSEIHI+VNSAGILDSKYPSLAGTAVEDW
Sbjct: 61  SAPLRRAVAVQADVSDPDQVKRLFDSAEKEFGSEIHILVNSAGILDSKYPSLAGTAVEDW 120

Query: 121 DEAFRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMAK 180
           DE FRVNCRGAFLVCKEA NR++RGGGGR++L+TTSIV SLPPGYGAYAASKAAVEAMAK
Sbjct: 121 DETFRVNCRGAFLVCKEAENRIKRGGGGRILLITTSIVLSLPPGYGAYAASKAAVEAMAK 180

Query: 181 IAAKELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGFL 240
           IAAKELKGT +TVNCVAPGPV TELFYAGKSEETVA++AEACP+ RLGQPDDVAKVVGFL
Sbjct: 181 IAAKELKGTGVTVNCVAPGPVETELFYAGKSEETVARIAEACPMVRLGQPDDVAKVVGFL 240

Query: 241 VTDAGGWVNGQVVRVNGGLVI 262
            TDAG WVNGQVVRVNGGLVI
Sbjct: 241 ATDAGEWVNGQVVRVNGGLVI 261

BLAST of Bhi03G001829 vs. NCBI nr
Match: XP_023518082.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 441.8 bits (1135), Expect = 4.1e-120
Identity = 227/262 (86.64%), Postives = 244/262 (93.13%), Query Frame = 0

Query: 1   MAAVSAA-LPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQ 60
           MAA +AA LPLDGR A+VTGASRGIGRAIAVHLH LGANLV+NYASNS+QA+LLASELNQ
Sbjct: 1   MAATAAAGLPLDGRNAVVTGASRGIGRAIAVHLHSLGANLVLNYASNSAQANLLASELNQ 60

Query: 61  SSAPLRRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVED 120
           SS P +R + VQADVSDP QVKRLFDSA +E GSEIHIVVN+AGILDSKYPSLAGTAVED
Sbjct: 61  SSTPRQRVIAVQADVSDPAQVKRLFDSAVEELGSEIHIVVNAAGILDSKYPSLAGTAVED 120

Query: 121 WDEAFRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMA 180
           WDE FRVNCRGAFLVCKEA+NRL+RGGGGR++L+TTSIVGSLPPGYGAYAASKAAVEAMA
Sbjct: 121 WDETFRVNCRGAFLVCKEAANRLKRGGGGRIILITTSIVGSLPPGYGAYAASKAAVEAMA 180

Query: 181 KIAAKELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGF 240
           K AAKELKGTEITVNCVAPGPVATELF+AGKSEE VA+  +ACPLGRLGQPDDVAKVVGF
Sbjct: 181 KTAAKELKGTEITVNCVAPGPVATELFFAGKSEEMVARSVDACPLGRLGQPDDVAKVVGF 240

Query: 241 LVTDAGGWVNGQVVRVNGGLVI 262
           L TD GGWVNGQVVRVNGGLVI
Sbjct: 241 LATDGGGWVNGQVVRVNGGLVI 262

BLAST of Bhi03G001829 vs. NCBI nr
Match: KAG6595510.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027496.1 NADPH-dependent aldehyde reductase-like protein, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 441.0 bits (1133), Expect = 7.0e-120
Identity = 223/259 (86.10%), Postives = 241/259 (93.05%), Query Frame = 0

Query: 3   AVSAALPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQSSA 62
           A +A LPLDGR A+VTGASRGIGRAIAVHLH LGANLV+NYASNS+QA++LASELNQSS 
Sbjct: 4   AAAAGLPLDGRNAVVTGASRGIGRAIAVHLHSLGANLVLNYASNSAQANILASELNQSST 63

Query: 63  PLRRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDWDE 122
           P +R + VQADVSDP QVKRLFDSA +E GSEIHIVVN+AGILDSKYPSLAGTAVEDWDE
Sbjct: 64  PRQRVIAVQADVSDPAQVKRLFDSAVEELGSEIHIVVNAAGILDSKYPSLAGTAVEDWDE 123

Query: 123 AFRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMAKIA 182
            FRVNCRGAFLVCKEA+NRL+RGGGGR++L+TTSIVGSLPPGYGAYAASKAAVEAMAK A
Sbjct: 124 TFRVNCRGAFLVCKEAANRLKRGGGGRIILITTSIVGSLPPGYGAYAASKAAVEAMAKTA 183

Query: 183 AKELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGFLVT 242
           AKELKGTEITVNCVAPGPVATELF+AGKSEE VA+  +ACPLGRLGQPDDVAKVVGFL T
Sbjct: 184 AKELKGTEITVNCVAPGPVATELFFAGKSEEMVARSVDACPLGRLGQPDDVAKVVGFLAT 243

Query: 243 DAGGWVNGQVVRVNGGLVI 262
           D GGWVNGQVVRVNGGLVI
Sbjct: 244 DGGGWVNGQVVRVNGGLVI 262

BLAST of Bhi03G001829 vs. ExPASy TrEMBL
Match: A0A0A0L1D5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G192180 PE=4 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 1.7e-124
Identity = 233/258 (90.31%), Postives = 246/258 (95.35%), Query Frame = 0

Query: 4   VSAALPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQSSAP 63
           ++ ALPLDGRTAIVTGASRGIGRAIA+HLH LGANLV+NYASNS+QADLLASELNQSSAP
Sbjct: 1   MATALPLDGRTAIVTGASRGIGRAIAIHLHSLGANLVLNYASNSTQADLLASELNQSSAP 60

Query: 64  LRRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDWDEA 123
           LRRAV VQADVSDPD VKRLFDSAEKEFGSEIHI+VNSAGILDSKYPSL  T VEDWDE 
Sbjct: 61  LRRAVAVQADVSDPDHVKRLFDSAEKEFGSEIHILVNSAGILDSKYPSLVETTVEDWDET 120

Query: 124 FRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMAKIAA 183
           FRVNCRGAFLVCKEA NR++RGGGGR+VL+TTSIV SLPPGYGAYAASKAAVEAMAKIAA
Sbjct: 121 FRVNCRGAFLVCKEAENRIKRGGGGRIVLITTSIVLSLPPGYGAYAASKAAVEAMAKIAA 180

Query: 184 KELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGFLVTD 243
           KELKGT ITVNCVAPGPVATELFYAGKSEETVA+MAEACP+GRLGQPDD+AKVVGFLVTD
Sbjct: 181 KELKGTGITVNCVAPGPVATELFYAGKSEETVARMAEACPMGRLGQPDDLAKVVGFLVTD 240

Query: 244 AGGWVNGQVVRVNGGLVI 262
           AG WVNGQVVRVNGGLVI
Sbjct: 241 AGEWVNGQVVRVNGGLVI 258

BLAST of Bhi03G001829 vs. ExPASy TrEMBL
Match: A0A1S3B4M7 (short-chain type dehydrogenase/reductase OS=Cucumis melo OX=3656 GN=LOC103485974 PE=4 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 8.6e-124
Identity = 232/261 (88.89%), Postives = 248/261 (95.02%), Query Frame = 0

Query: 1   MAAVSAALPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQS 60
           M+ V+A LPL GRTAIVTGASRGIGRAIA+HLH LGANLV+NYASNS+QADLLAS+LNQS
Sbjct: 1   MSTVAAPLPLHGRTAIVTGASRGIGRAIAIHLHSLGANLVLNYASNSTQADLLASDLNQS 60

Query: 61  SAPLRRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDW 120
           SAPLRRAV VQADVSDPDQVKRLFDSAEKEFGSEIHI+VNSAGILDSKYPSLAGTAVEDW
Sbjct: 61  SAPLRRAVAVQADVSDPDQVKRLFDSAEKEFGSEIHILVNSAGILDSKYPSLAGTAVEDW 120

Query: 121 DEAFRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMAK 180
           DE FRVNCRGAFLVCKEA NR++RGGGGR++L+TTSIV SLPPGYGAYAASKAAVEAMAK
Sbjct: 121 DETFRVNCRGAFLVCKEAENRIKRGGGGRILLITTSIVLSLPPGYGAYAASKAAVEAMAK 180

Query: 181 IAAKELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGFL 240
           IAAKELKGT +TVNCVAPGPV TELFYAGKSEETVA++AEACP+ RLGQPDDVAKVVGFL
Sbjct: 181 IAAKELKGTGVTVNCVAPGPVETELFYAGKSEETVARIAEACPMVRLGQPDDVAKVVGFL 240

Query: 241 VTDAGGWVNGQVVRVNGGLVI 262
            TDAG WVNGQVVRVNGGLVI
Sbjct: 241 ATDAGEWVNGQVVRVNGGLVI 261

BLAST of Bhi03G001829 vs. ExPASy TrEMBL
Match: A0A6J1EGB3 (NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111432299 PE=4 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 1.3e-119
Identity = 221/257 (85.99%), Postives = 240/257 (93.39%), Query Frame = 0

Query: 5   SAALPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQSSAPL 64
           +A LPLDGR A+VTGASRGIGRAIAVHLH LGANLV+NYASNS+QA++LASELNQSS P 
Sbjct: 6   AAGLPLDGRNAVVTGASRGIGRAIAVHLHSLGANLVLNYASNSAQANILASELNQSSTPR 65

Query: 65  RRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDWDEAF 124
           +R + VQADVSDP QVKRLFDSA +E GSEIHIVVN+AGILDSKYPSLAGTA+EDWDE F
Sbjct: 66  QRVIAVQADVSDPAQVKRLFDSAVEELGSEIHIVVNAAGILDSKYPSLAGTALEDWDETF 125

Query: 125 RVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMAKIAAK 184
           RVNCRGAFLVCKEA+NRL+RGGGGR++L+TTSIVGSLPPGYGAYAASKAAVEAMAK AAK
Sbjct: 126 RVNCRGAFLVCKEAANRLKRGGGGRIILITTSIVGSLPPGYGAYAASKAAVEAMAKTAAK 185

Query: 185 ELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGFLVTDA 244
           ELKGTEITVNCVAPGPVATELF+AGKSEE VA+  +ACPLGRLGQPDDVAKVVGFL TD 
Sbjct: 186 ELKGTEITVNCVAPGPVATELFFAGKSEEMVARSVDACPLGRLGQPDDVAKVVGFLATDG 245

Query: 245 GGWVNGQVVRVNGGLVI 262
           GGWVNGQVVRVNGGLVI
Sbjct: 246 GGWVNGQVVRVNGGLVI 262

BLAST of Bhi03G001829 vs. ExPASy TrEMBL
Match: A0A5A7V0W1 (Short-chain type dehydrogenase/reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00450 PE=4 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 2.9e-119
Identity = 224/255 (87.84%), Postives = 240/255 (94.12%), Query Frame = 0

Query: 1   MAAVSAALPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQS 60
           M+ V+A LPL GRTAIVTGASRGIGRAIA+HLH LGANLV+NYASNS+QADLLAS+LNQS
Sbjct: 1   MSTVAAPLPLHGRTAIVTGASRGIGRAIAIHLHSLGANLVLNYASNSTQADLLASDLNQS 60

Query: 61  SAPLRRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDW 120
           SAPLRRAV VQADVSDPDQVKRLFDSAEKEFGSEIHI+VNSAGILDSKYPSLAGTAVEDW
Sbjct: 61  SAPLRRAVAVQADVSDPDQVKRLFDSAEKEFGSEIHILVNSAGILDSKYPSLAGTAVEDW 120

Query: 121 DEAFRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMAK 180
           DE FRVNCRGAFLVCKEA NR++RGGGGR++L+TTSIV SLPPGYGAYAASKAAVEAMAK
Sbjct: 121 DETFRVNCRGAFLVCKEAENRIKRGGGGRILLITTSIVLSLPPGYGAYAASKAAVEAMAK 180

Query: 181 IAAKELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGFL 240
           IAAKELKGT +TVNCVAPGPV TELFYAGKSEETVA++AEACP+ RLGQPDDVAKVVGFL
Sbjct: 181 IAAKELKGTGVTVNCVAPGPVETELFYAGKSEETVARIAEACPMVRLGQPDDVAKVVGFL 240

Query: 241 VTDAGGWVNGQVVRV 256
            TDAG WVNGQ  RV
Sbjct: 241 ATDAGEWVNGQGTRV 255

BLAST of Bhi03G001829 vs. ExPASy TrEMBL
Match: A0A6J1HS50 (NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466086 PE=4 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 8.3e-119
Identity = 222/259 (85.71%), Postives = 239/259 (92.28%), Query Frame = 0

Query: 3   AVSAALPLDGRTAIVTGASRGIGRAIAVHLHFLGANLVINYASNSSQADLLASELNQSSA 62
           A +AALPLDGR A+VTGASRGIGRAIAVHLH LG NLV+NYASNS+QA+LLASELNQSS 
Sbjct: 2   ATAAALPLDGRNAVVTGASRGIGRAIAVHLHSLGVNLVLNYASNSAQANLLASELNQSST 61

Query: 63  PLRRAVVVQADVSDPDQVKRLFDSAEKEFGSEIHIVVNSAGILDSKYPSLAGTAVEDWDE 122
           P +R + VQADVSDP QVKRLFDSA +E GSEIHIVVN+AGILDSKYPSLA TAVEDWDE
Sbjct: 62  PRQRVIAVQADVSDPAQVKRLFDSAVEELGSEIHIVVNAAGILDSKYPSLARTAVEDWDE 121

Query: 123 AFRVNCRGAFLVCKEASNRLRRGGGGRVVLLTTSIVGSLPPGYGAYAASKAAVEAMAKIA 182
            FRVNCRGAFLVCKEA+NRL+RGGGGR++L+TTSIVGSLPPGYGAYAASKAAVEAMAK A
Sbjct: 122 TFRVNCRGAFLVCKEAANRLKRGGGGRIILITTSIVGSLPPGYGAYAASKAAVEAMAKTA 181

Query: 183 AKELKGTEITVNCVAPGPVATELFYAGKSEETVAKMAEACPLGRLGQPDDVAKVVGFLVT 242
           AKELKGTEITVNCVAPGPVATELF+AGKSEE VA+  +ACPLGRLGQPDDVAKVV FL T
Sbjct: 182 AKELKGTEITVNCVAPGPVATELFFAGKSEEMVARSVDACPLGRLGQPDDVAKVVAFLAT 241

Query: 243 DAGGWVNGQVVRVNGGLVI 262
           D GGWVNGQVVRVNGGLVI
Sbjct: 242 DGGGWVNGQVVRVNGGLVI 260

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G13180.11.1e-9464.20NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G03980.11.5e-8361.30NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G18210.14.1e-7860.91NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G04000.18.0e-7454.04NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G24360.12.8e-2632.28NAD(P)-binding Rossmann-fold superfamily protein [more]
Match NameE-valueIdentityDescription
Q9SQR42.1e-8261.30NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Arabidopsis th... [more]
Q086323.9e-7357.42Short-chain type dehydrogenase/reductase OS=Picea abies OX=3329 PE=2 SV=1[more]
Q9SQR21.1e-7254.04NADPH-dependent aldehyde reductase 2, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
P0CU751.3e-3937.93Short chain dehydrogenase claC OS=Passalora fulva OX=5499 GN=claC PE=1 SV=1[more]
Q007912.8e-3938.55Versicolorin reductase OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS... [more]
Match NameE-valueIdentityDescription
XP_038883178.13.7e-137100.00NADPH-dependent aldehyde reductase-like protein, chloroplastic [Benincasa hispid... [more]
XP_004147531.13.6e-12490.31NADPH-dependent aldehyde reductase-like protein, chloroplastic [Cucumis sativus]... [more]
XP_008441973.11.8e-12388.89PREDICTED: short-chain type dehydrogenase/reductase [Cucumis melo][more]
XP_023518082.14.1e-12086.64NADPH-dependent aldehyde reductase-like protein, chloroplastic [Cucurbita pepo s... [more]
KAG6595510.17.0e-12086.10NADPH-dependent aldehyde reductase-like protein, chloroplastic, partial [Cucurbi... [more]
Match NameE-valueIdentityDescription
A0A0A0L1D51.7e-12490.31Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G192180 PE=4 SV=1[more]
A0A1S3B4M78.6e-12488.89short-chain type dehydrogenase/reductase OS=Cucumis melo OX=3656 GN=LOC103485974... [more]
A0A6J1EGB31.3e-11985.99NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Cucurbita mosc... [more]
A0A5A7V0W12.9e-11987.84Short-chain type dehydrogenase/reductase OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A6J1HS508.3e-11985.71NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Cucurbita maxi... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 168..187
score: 37.68
coord: 93..104
score: 39.09
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 189..206
score: 41.71
coord: 93..104
score: 38.37
coord: 14..31
score: 51.92
coord: 142..158
score: 17.17
coord: 168..187
score: 39.06
coord: 223..243
score: 38.94
NoneNo IPR availableSMARTSM00822PKS_KRcoord: 13..199
e-value: 1.5E-4
score: -37.2
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 19..259
e-value: 1.9E-58
score: 197.8
NoneNo IPR availableGENE3D3.40.50.720coord: 2..259
e-value: 2.4E-77
score: 261.9
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 16..206
e-value: 1.2E-12
score: 45.5
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 10..259
e-value: 4.1E-20
score: 69.8
NoneNo IPR availablePANTHERPTHR48107:SF12SHORT-CHAIN TYPE DEHYDROGENASE/REDUCTASEcoord: 3..261
NoneNo IPR availablePANTHERPTHR48107NADPH-DEPENDENT ALDEHYDE REDUCTASE-LIKE PROTEIN, CHLOROPLASTIC-RELATEDcoord: 3..261
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 11..260

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M001829Bhi03M001829mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004316 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
molecular_function GO:0016491 oxidoreductase activity