Bhi02G001178 (gene) Wax gourd (B227) v1

Overview


Name	Bhi02G001178
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	DNA-directed RNA polymerase
Location	chr2: 36279788 .. 36280117 (-)
RNA-Seq Expression	Bhi02G001178
Synteny	Bhi02G001178

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCAATCTAGCAAAAGCTTACGATGGCTATCAATTTTATTTACCAGCTTTTCTGGATTTTCGTGGTAGAATCTACCACAATGGTGGCTTGCATTTCCATGATCGTGATCTAGCAAGAAGCTTGATAGTCTTTGTGGGTGAAAGTAAGAAAACAATCCGTAGTGAGATGGACTTCCGCAAAAATGCTAATTGTGCTATAACGTTTCATTACAAGCCTTTCGAGACCCATCAAAAGATTGATCATTCGTTAAATGCTAATGTATTTGGCAAAAGTAAGAAAGATTTGATTGAGTATTCTCGAAAAGCAAAACACCATAATGCTAGATAA

mRNA sequence

ATGCTCAATCTAGCAAAAGCTTACGATGGCTATCAATTTTATTTACCAGCTTTTCTGGATTTTCGTGGTAGAATCTACCACAATGGTGGCTTGCATTTCCATGATCGTGATCTAGCAAGAAGCTTGATAGTCTTTGTGGGTGAAAGTAAGAAAACAATCCGTAGTGAGATGGACTTCCGCAAAAATGCTAATTGTGCTATAACGTTTCATTACAAGCCTTTCGAGACCCATCAAAAGATTGATCATTCGTTAAATGCTAATGTATTTGGCAAAAGTAAGAAAGATTTGATTGAGTATTCTCGAAAAGCAAAACACCATAATGCTAGATAA

Coding sequence (CDS)

ATGCTCAATCTAGCAAAAGCTTACGATGGCTATCAATTTTATTTACCAGCTTTTCTGGATTTTCGTGGTAGAATCTACCACAATGGTGGCTTGCATTTCCATGATCGTGATCTAGCAAGAAGCTTGATAGTCTTTGTGGGTGAAAGTAAGAAAACAATCCGTAGTGAGATGGACTTCCGCAAAAATGCTAATTGTGCTATAACGTTTCATTACAAGCCTTTCGAGACCCATCAAAAGATTGATCATTCGTTAAATGCTAATGTATTTGGCAAAAGTAAGAAAGATTTGATTGAGTATTCTCGAAAAGCAAAACACCATAATGCTAGATAA

Protein sequence

MLNLAKAYDGYQFYLPAFLDFRGRIYHNGGLHFHDRDLARSLIVFVGESKKTIRSEMDFRKNANCAITFHYKPFETHQKIDHSLNANVFGKSKKDLIEYSRKAKHHNAR

Homology

[Close]

BLAST of Bhi02G001178 vs. ExPASy Swiss-Prot
Match: P10581 (Probable DNA-directed RNA polymerase OS=Zea mays OX=4577 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 2.8e-09
Identity = 33/73 (45.21%), Postives = 44/73 (60.27%), Query Frame = 0


Query: 2   LNLAKAYDGYQFYLPAFLDFRGRIYHNGGLHFHDRDLARSLIVF---VGESKKTIRSEMD 61
           + +A+AY  Y+ Y P FLDFRGR Y +G  HFH+RDL RSLI+F      +  TI S++ 
Sbjct: 518 MKIAEAYLDYKIYFPIFLDFRGRNYRHGPFHFHERDLVRSLIIFDESDDSAAHTINSDVG 577

Query: 62  FRKNANCAITFHY 72
            R   N  I+  Y
Sbjct: 578 DRILHNFLISAAY 590

[Close]

BLAST of Bhi02G001178 vs. ExPASy Swiss-Prot
Match: P33539 (Probable DNA-directed RNA polymerase OS=Agaricus bitorquis OX=5343 PE=3 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 6.1e-04
Identity = 21/46 (45.65%), Postives = 32/46 (69.57%), Query Frame = 0


Query: 1   MLNLAKAYDGYQFYLPAFLDFRGRIYH-NGGLHFHDRDLARSLIVF 46
           +++LA  Y   +FYLP ++DFRGR+Y  +  + +   DLARSLI+F
Sbjct: 595 IISLASLYKDNEFYLPVYVDFRGRVYPLSNYISYQGGDLARSLILF 640

[Close]

BLAST of Bhi02G001178 vs. NCBI nr
Match: XP_020594099.1 (uncharacterized protein LOC110034152 [Phalaenopsis equestris])

HSP 1 Score: 106.7 bits (265), Expect = 1.3e-19
Identity = 60/108 (55.56%), Postives = 75/108 (69.44%), Query Frame = 0


Query: 1   MLNLAKAYDGYQFYLPAFLDFRGRIYHNGGLHFHDRDLARSLIVFVG-ESKKTIRSEMDF 60
           ++ LA AYDGY FYLPAFLDFRGRIY +G LHFH+RDLARSLI+F G ESK +I  EM  
Sbjct: 234 IIKLAIAYDGYHFYLPAFLDFRGRIYRSGILHFHERDLARSLILFAGTESKDSISEEMKI 293

Query: 61  RKNANCAITFHYKPFETHQKIDHSLN--ANVFGKSKKDLIEYSRKAKH 106
           +     A  FHYK F T   +DH+     N F   +K+LI+++R+AKH
Sbjct: 294 Q--IMVAAAFHYKKFIT---VDHAKEWCLNNFYNLEKNLIDFAREAKH 336

[Close]

BLAST of Bhi02G001178 vs. NCBI nr
Match: XP_020251012.1 (uncharacterized protein LOC109828426, partial [Asparagus officinalis])

HSP 1 Score: 99.4 bits (246), Expect = 2.1e-17
Identity = 54/105 (51.43%), Postives = 69/105 (65.71%), Query Frame = 0


Query: 1   MLNLAKAYDGYQFYLPAFLDFRGRIYHNGGLHFHDRDLARSLIVFVGESKKTIRSEMDFR 60
           ++ LAKAYDGY FYLPAFLDFRGRIY +G LHFH+RDLARSLI+F G   K  +   +  
Sbjct: 461 IIKLAKAYDGYHFYLPAFLDFRGRIYRSGILHFHERDLARSLILFAG--TKFEKPLSNIY 520

Query: 61  KNANCAITFHYKPFETHQKIDHSLNANVFGKSKKDLIEYSRKAKH 106
            N   A  FHYK F T     +    N+  K K++ I+++R+AKH
Sbjct: 521 NNIIDATAFHYKKFTTVNDAKYWFLNNI-DKIKRNPIDFAREAKH 562

[Close]

BLAST of Bhi02G001178 vs. NCBI nr
Match: YP_009315942.1 (DNA-dependent RNA polymerase [Cocos nucifera] >AOX12936.1 DNA-dependent RNA polymerase [Cocos nucifera])

HSP 1 Score: 99.0 bits (245), Expect = 2.7e-17
Identity = 56/110 (50.91%), Postives = 72/110 (65.45%), Query Frame = 0


Query: 1   MLNLAKAYDGYQFYLPAFLDFRGRIYHNGGLHFHDRDLARSLIVFVGESKKTIRSEMDFR 60
           +L LA AYDGY+FYLPAFLDFRGRIY +G LHFH+RD+ARSLIVF   + K+  S  + R
Sbjct: 429 ILTLASAYDGYKFYLPAFLDFRGRIYRSGILHFHERDIARSLIVFANNNNKS--SSYNCR 488

Query: 61  KNANCAITFHYKPFETHQKIDHSL-----NANVFGKSKKDLIEYSRKAKH 106
                A  FHYK F +   I  S+     N +VF  S   LI++++KAK+
Sbjct: 489 TVLISAAPFHYKSFVS---ITDSIQWYIDNKSVFNTSDDSLIQFAQKAKN 533

[Close]

BLAST of Bhi02G001178 vs. NCBI nr
Match: XP_020547357.1 (uncharacterized protein LOC110011516 [Sesamum indicum])

HSP 1 Score: 97.1 bits (240), Expect = 1.0e-16
Identity = 52/102 (50.98%), Postives = 67/102 (65.69%), Query Frame = 0


Query: 7   AYDGYQFYLPAFLDFRGRIYHNGGLHFHDRDLARSLIVFVGESKKTIRSEMD--FRKNAN 66
           AY+GYQFYLP+F+DFRGRIY +G LHFH+RDLARSLIVF      +  SE+D   RK   
Sbjct: 56  AYEGYQFYLPSFIDFRGRIYRSGILHFHERDLARSLIVFAPNPYDSYDSEIDKRCRKILY 115

Query: 67  CAITFHYKPFETHQKIDHSLNAN--VFGKSKKDLIEYSRKAK 105
           C+  FHYK F+++ + +   N N   F  S   LIE++  AK
Sbjct: 116 CSAPFHYKSFQSYTESNEWYNDNKSSFNTSDHSLIEFALHAK 157

[Close]

BLAST of Bhi02G001178 vs. NCBI nr
Match: PRQ23030.1 (putative DNA-directed RNA polymerase [Rosa chinensis])

HSP 1 Score: 95.5 bits (236), Expect = 3.0e-16
Identity = 47/81 (58.02%), Postives = 58/81 (71.60%), Query Frame = 0


Query: 1   MLNLAKAYDGYQFYLPAFLDFRGRIYHNGGLHFHDRDLARSLIVFVGESKKTIRSEMDFR 60
           ++NLA AY+GY+FYLPAFLDFRGRIY  G LHFH+RDLARSLIVF  +SKK +   M+  
Sbjct: 493 VINLASAYEGYKFYLPAFLDFRGRIYRAGVLHFHERDLARSLIVFSNKSKKFVSMSMEDH 552

Query: 61  KNANCAIT----FHYKPFETH 78
           K     IT    FH+K F ++
Sbjct: 553 KFLTAMITSAAAFHFKKFTSN 573

[Close]

BLAST of Bhi02G001178 vs. ExPASy TrEMBL
Match: A0A2Z2CD19 (DNA-directed RNA polymerase OS=Cocos nucifera OX=13894 GN=RNA_pol PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.3e-17
Identity = 56/110 (50.91%), Postives = 72/110 (65.45%), Query Frame = 0


Query: 1   MLNLAKAYDGYQFYLPAFLDFRGRIYHNGGLHFHDRDLARSLIVFVGESKKTIRSEMDFR 60
           +L LA AYDGY+FYLPAFLDFRGRIY +G LHFH+RD+ARSLIVF   + K+  S  + R
Sbjct: 429 ILTLASAYDGYKFYLPAFLDFRGRIYRSGILHFHERDIARSLIVFANNNNKS--SSYNCR 488

Query: 61  KNANCAITFHYKPFETHQKIDHSL-----NANVFGKSKKDLIEYSRKAKH 106
                A  FHYK F +   I  S+     N +VF  S   LI++++KAK+
Sbjct: 489 TVLISAAPFHYKSFVS---ITDSIQWYIDNKSVFNTSDDSLIQFAQKAKN 533

[Close]

BLAST of Bhi02G001178 vs. ExPASy TrEMBL
Match: A0A2P6PM98 (DNA-directed RNA polymerase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr6g0256711 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.4e-16
Identity = 47/81 (58.02%), Postives = 58/81 (71.60%), Query Frame = 0


Query: 1   MLNLAKAYDGYQFYLPAFLDFRGRIYHNGGLHFHDRDLARSLIVFVGESKKTIRSEMDFR 60
           ++NLA AY+GY+FYLPAFLDFRGRIY  G LHFH+RDLARSLIVF  +SKK +   M+  
Sbjct: 493 VINLASAYEGYKFYLPAFLDFRGRIYRAGVLHFHERDLARSLIVFSNKSKKFVSMSMEDH 552

Query: 61  KNANCAIT----FHYKPFETH 78
           K     IT    FH+K F ++
Sbjct: 553 KFLTAMITSAAAFHFKKFTSN 573

[Close]

BLAST of Bhi02G001178 vs. ExPASy TrEMBL
Match: A0A6M9TTE7 (DNA-directed RNA polymerase OS=Mirabilis himalaica OX=482968 GN=ORF312 PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 2.5e-16
Identity = 49/81 (60.49%), Postives = 58/81 (71.60%), Query Frame = 0


Query: 1   MLNLAKAYDGYQFYLPAFLDFRGRIYHNGGLHFHDRDLARSLIVFVGESKKTIRSEMDFR 60
           +LNLA AY+GY FYLPAFLDFRGRIY  G LHFH+RDLARSLIVF G+ +KT        
Sbjct: 54  ILNLASAYEGYTFYLPAFLDFRGRIYRCGILHFHERDLARSLIVFAGDDEKT------NT 113

Query: 61  KNANCAI----TFHYKPFETH 78
           K  +CA+     FHYK FE++
Sbjct: 114 KVNSCAVISAFAFHYKSFESY 128

[Close]

BLAST of Bhi02G001178 vs. ExPASy TrEMBL
Match: A0A6J5Y9W3 (DNA-directed RNA polymerase OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS3511 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.6e-15
Identity = 53/106 (50.00%), Postives = 68/106 (64.15%), Query Frame = 0


Query: 1   MLNLAKAYDGYQFYLPAFLDFRGRIYHNGGLHFHDRDLARSLIVFVGESKKTIRSEMDFR 60
           ++ LA+AY+GY FYLPAFLDFRGRIY +G LHFH+RDLARS+IVF       I+S  +  
Sbjct: 424 IMKLAQAYEGYHFYLPAFLDFRGRIYRSGVLHFHERDLARSMIVFA-----DIKSSGNID 483

Query: 61  KNAN-CAITFHYKPFETHQKIDHSLNANVFGKS-KKDLIEYSRKAK 105
            NA   A  FHYK F +  +  +  N N    S   DL+ Y+R+AK
Sbjct: 484 MNAYLAAAAFHYKSFVSVDEALYFSNNNFLQLSHDDDLLMYAREAK 524

[Close]

BLAST of Bhi02G001178 vs. ExPASy TrEMBL
Match: A0A6J1IE03 (DNA-directed RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111474651 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.6e-15
Identity = 43/87 (49.43%), Postives = 59/87 (67.82%), Query Frame = 0


Query: 1   MLNLAKAYDGYQFYLPAFLDFRGRIYHNGGLHFHDRDLARSLIVFVGESKKTIRSEMDFR 60
           +LNLA+ YDGY+FYLPAFLDFRGRIY +G LHFH+ DLARSLIVF  ++++ I+     R
Sbjct: 46  ILNLARVYDGYKFYLPAFLDFRGRIYRSGVLHFHECDLARSLIVFANDNQEEIKCNSLIR 105

Query: 61  KNANCAITFHYKPFETHQKIDHSLNAN 88
                +  FH++ F +  +    +N N
Sbjct: 106 DQVELSTFFHFQSFNSMLEAVDFVNNN 132

The following BLAST results are available for this feature:

BLAST of Bhi02G001178 vs.


Match Name	E-value	Identity	Description

BLAST of Bhi02G001178 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P10581	2.8e-09	45.21	Probable DNA-directed RNA polymerase OS=Zea mays OX=4577 PE=3 SV=1	[more]
P33539	6.1e-04	45.65	Probable DNA-directed RNA polymerase OS=Agaricus bitorquis OX=5343 PE=3 SV=1	[more]

BLAST of Bhi02G001178 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_020594099.1	1.3e-19	55.56	uncharacterized protein LOC110034152 [Phalaenopsis equestris]	[more]
XP_020251012.1	2.1e-17	51.43	uncharacterized protein LOC109828426, partial [Asparagus officinalis]	[more]
YP_009315942.1	2.7e-17	50.91	DNA-dependent RNA polymerase [Cocos nucifera] >AOX12936.1 DNA-dependent RNA poly...	[more]
XP_020547357.1	1.0e-16	50.98	uncharacterized protein LOC110011516 [Sesamum indicum]	[more]
PRQ23030.1	3.0e-16	58.02	putative DNA-directed RNA polymerase [Rosa chinensis]	[more]

BLAST of Bhi02G001178 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2Z2CD19	1.3e-17	50.91	DNA-directed RNA polymerase OS=Cocos nucifera OX=13894 GN=RNA_pol PE=3 SV=1	[more]
A0A2P6PM98	1.4e-16	58.02	DNA-directed RNA polymerase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr6g0256711 ...	[more]
A0A6M9TTE7	2.5e-16	60.49	DNA-directed RNA polymerase OS=Mirabilis himalaica OX=482968 GN=ORF312 PE=3 SV=1	[more]
A0A6J5Y9W3	1.6e-15	50.00	DNA-directed RNA polymerase OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS3511 PE=...	[more]
A0A6J1IE03	1.6e-15	49.43	DNA-directed RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111474651 PE=3 SV=...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.70.370		coord: 20..102 e-value: 5.6E-5 score: 25.4
None	No IPR available	PANTHER	PTHR10102:SF8	DNA-DIRECTED RNA POLYMERASE	coord: 7..85
IPR002092	DNA-directed RNA polymerase, phage-type	PANTHER	PTHR10102	DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL	coord: 7..85
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 1..50

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi02M001178	Bhi02M001178	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity