Bhi01G001263 (gene) Wax gourd (B227) v1

BLAST of Bhi01G001263 vs. NCBI nr
Match: XP_038880648.1 (uncharacterized protein LOC120072275 [Benincasa hispida])

HSP 1 Score: 1314.3 bits (3400), Expect = 0.0e+00
Identity = 655/668 (98.05%), Postives = 655/668 (98.05%), Query Frame = 0

Query: 1   MVDFFMTTDKQMEEPKSRKPRQRRNLQMEELPTFTKWLNSVGHSGSCNDANSKSKPVNGN 60
           MVDFFMTTDKQMEEPKSRKPRQRRNLQMEELPTFTKWLNSVGHSGSCNDANSKSKPVNGN
Sbjct: 1   MVDFFMTTDKQMEEPKSRKPRQRRNLQMEELPTFTKWLNSVGHSGSCNDANSKSKPVNGN 60

Query: 61  RPVVRTPVVVFDSSEDGGAVVADVPKDRVQAVDDSISARAGCCCWQSSKSTRRECALKFH 120
           RPVVRTPVVVFDSSEDGGAVVADVPKDRVQAVDDSISARAGCCCWQSSKSTRRECALKFH
Sbjct: 61  RPVVRTPVVVFDSSEDGGAVVADVPKDRVQAVDDSISARAGCCCWQSSKSTRRECALKFH 120

Query: 121 LSLRKRKVVTNGSEVEVVTVVSNLREEETVVAVSDVLEKDGCGCRCFPTFQICGRRKSIV 180
           LSLRKRKVVTNGSEVEVVTVVSNLREEETVVAVSDVLEKDGCGCRCFPTFQICGRRKSIV
Sbjct: 121 LSLRKRKVVTNGSEVEVVTVVSNLREEETVVAVSDVLEKDGCGCRCFPTFQICGRRKSIV 180

Query: 181 VLQKEDGAVTDDPNLRTEEVANRGPDLVKEEEVVVVGGSDLRKEEGSGCCCGRCGCFPAF 240
           VLQKEDGAVTDDPNLRTEEVANRGPDLVKEEEVVVVGGSDLRKEEGSGCCCGRCGCFPAF
Sbjct: 181 VLQKEDGAVTDDPNLRTEEVANRGPDLVKEEEVVVVGGSDLRKEEGSGCCCGRCGCFPAF 240

Query: 241 QICRRRNVVASKEEAVVDVPEVEEEVANDKVNKQEGDSVECLQAFQSHICCAGRKTVDDN 300
           QICRRRNVVASKEEAVVDVPEVEEEVANDKVNKQEGDSVECLQAFQSHICCAGRKTVDDN
Sbjct: 241 QICRRRNVVASKEEAVVDVPEVEEEVANDKVNKQEGDSVECLQAFQSHICCAGRKTVDDN 300

Query: 301 PKTFEKEALASEDSSNVDVPDLQKEGSGGCCSCFKCMPTLHICGRRRNAVSEVPNPGREE 360
           PKTFEKEALASEDSSNVDVPDLQKEGSGGCCSCFKCMPTLHICGRRRNAVSEVPNPGREE
Sbjct: 301 PKTFEKEALASEDSSNVDVPDLQKEGSGGCCSCFKCMPTLHICGRRRNAVSEVPNPGREE 360

Query: 361 KVVVSVSDPPEGEEVVAADEEWHSKSTQGGICWPFQICTRGWLPRFFLCGERITVDASNH 420
           KVVVSVSDPPEGEEVVAADEEWHSKSTQGGICWPFQICTRGWLPRFFLCGERITVDASNH
Sbjct: 361 KVVVSVSDPPEGEEVVAADEEWHSKSTQGGICWPFQICTRGWLPRFFLCGERITVDASNH 420

Query: 421 REEEEKAPPDVQKEEVVAVIPDPQKESIAVADGIPDDGKEKQVAADDIPVQSTEEKMSAG 480
           REEEEKAPPDVQKEEVVAVIPDPQKESIAVADGIPDDGKEKQVAADDIPVQSTEEKMSAG
Sbjct: 421 REEEEKAPPDVQKEEVVAVIPDPQKESIAVADGIPDDGKEKQVAADDIPVQSTEEKMSAG 480

Query: 481 KEENASSGSIQETFEPDLNQEEIVDSGVSDLNQEDEGGCCRCFKLGGKEGSRRQHRRSPK 540
           KEENASSGSIQETFEP             DLNQEDEGGCCRCFKLGGKEGSRRQHRRSPK
Sbjct: 481 KEENASSGSIQETFEP-------------DLNQEDEGGCCRCFKLGGKEGSRRQHRRSPK 540

Query: 541 SREGGWGFQICGWLPTLDMCRGRKKESVSIAKLHEKEGLVDNGVSEVHNEVVDAAGVTGV 600
           SREGGWGFQICGWLPTLDMCRGRKKESVSIAKLHEKEGLVDNGVSEVHNEVVDAAGVTGV
Sbjct: 541 SREGGWGFQICGWLPTLDMCRGRKKESVSIAKLHEKEGLVDNGVSEVHNEVVDAAGVTGV 600

Query: 601 VADTDHSNSTRCCGCWHSKPRRRRAVDVVKDGGSGRCSKSKRRKGWLRRWGRKQREGKER 660
           VADTDHSNSTRCCGCWHSKPRRRRAVDVVKDGGSGRCSKSKRRKGWLRRWGRKQREGKER
Sbjct: 601 VADTDHSNSTRCCGCWHSKPRRRRAVDVVKDGGSGRCSKSKRRKGWLRRWGRKQREGKER 655

Query: 661 KKKKFKIF 669
           KKKKFKIF
Sbjct: 661 KKKKFKIF 655

BLAST of Bhi01G001263 vs. NCBI nr
Match: KGN57524.1 (hypothetical protein Csa_011487 [Cucumis sativus])

HSP 1 Score: 681.8 bits (1758), Expect = 6.0e-192
Identity = 416/680 (61.18%), Postives = 465/680 (68.38%), Query Frame = 0

Query: 6   MTTDKQMEEPKSRKPRQRRNLQMEELPTFTKWLNSVGHSGSCNDANSKSKPVN-GNRPVV 65
           M T+K  EE +SRKPR RRN QMEE PTFTKWL + GHSGSCNDA SKSK +N  NRP+ 
Sbjct: 1   MATNKPKEEQRSRKPRHRRNQQMEEFPTFTKWLTTFGHSGSCNDAKSKSKQLNPANRPLQ 60

Query: 66  RTPVVVFDSSEDGGAVVADVPKD---RVQAVDDSISAR--AGCCCWQSSKSTRRECALKF 125
           R PVV+  SSED   VV +VP++   +  AVD SISAR  AGCCCWQSSKSTRRECALKF
Sbjct: 61  RPPVVLPASSEDAVPVVTNVPEEEGVQTMAVDGSISARGGAGCCCWQSSKSTRRECALKF 120

Query: 126 HLSLRKRKVVTNGSEVEVVTVVSNLREEETVVAVSDVLEKDGCGCRCFPTFQICGRRKSI 185
           H+SLRKRKVV N +E EVV  V N  EE TV  V     KDGCGCRC  TF+I  RRKS 
Sbjct: 121 HISLRKRKVVANSTEAEVVAAVLNPPEEATVEDV-----KDGCGCRCLRTFKIFRRRKSR 180

Query: 186 VV----LQKEDGAVTDDPNLRTEEVANRGPDLVKEEEVVVVGGSDLRKEEGSGCCCGRCG 245
           VV    LQKE+GAVTD  NLRTEEVA+ G D++KEEEVV+    D RKEE SGCCCGR  
Sbjct: 181 VVGVSDLQKEEGAVTDGVNLRTEEVASSGSDMMKEEEVVI--APDRRKEEESGCCCGRWK 240

Query: 246 CFPAFQICRRRNVVASKEEAVVDVPEVEEEVANDKVNKQEGDSVECLQAFQSHICCAGRK 305
           C P FQICRRR VVA KEE V   P+V EEV ND V KQE DSV CLQAF  HI C GRK
Sbjct: 241 CSPTFQICRRRKVVAGKEEVVGGAPKV-EEVGNDNVTKQEEDSVGCLQAF--HI-CGGRK 300

Query: 306 TVDDNPKTFEKEALASEDSSNVDVPDLQKEGSGGCCSCFKCMPTLHIC-GRRRNAVSEVP 365
            VDDNPKT EKE L S DSSN+DV +LQKE S GCCSCF+C+PT  IC GRR N  S VP
Sbjct: 301 RVDDNPKTSEKEPLVSNDSSNLDVQNLQKEES-GCCSCFRCIPTFQICGGRRSNEDSGVP 360

Query: 366 NPGREEKVVVSVSDPPEGEEVVAADEEWHSKSTQGGICWPFQICTRGWLPRFFLCGERIT 425
            PGREEKV+V VSDPPE   VV   E  HS+  QGG CW       GW PRF LCGE   
Sbjct: 361 KPGREEKVIVDVSDPPEMGSVVDGRER-HSRPVQGGTCW------SGWFPRFLLCGEGTA 420

Query: 426 VDASNHREEEEKAPPDVQKEEVVAVIPDPQKESIAVADGIPDDGKEKQVAADDIPVQSTE 485
           VDA NHREEEEKAP D +KEE V V       + AV D I D  KEK VAA DIPV + E
Sbjct: 421 VDAPNHREEEEKAPSDARKEEKVVV-------ATAVGDEISDHDKEKPVAAIDIPVVNEE 480

Query: 486 E-------KMSAGKEENASSGSIQETFEPDLNQEEIVDSGVSDLNQEDEGGCCRCFKLGG 545
           E        +   KE+N SS +IQ     D+ +EEIVDS     +++ EGG C C+   G
Sbjct: 481 EVFVGAGDTLDLHKEKNVSSCNIQ-----DVRKEEIVDS-----DEKVEGGGCGCW---G 540

Query: 546 KEGSRRQHRRSPKSREGGWGFQIC--GWLPTLDMCRGRKKESVSIAKLHEKEGLVDNGVS 605
           KE   RQ  RS +S EG W FQIC  G LPTL++CRGRK  SV I+KL E EGLVDN VS
Sbjct: 541 KESGSRQQHRSSRSMEGCWSFQICGRGCLPTLNICRGRKDVSVRISKLVE-EGLVDNDVS 600

Query: 606 EVHNEVVDAAGVTGVVADTDHSNSTRCCGCWHSKPRRRRAVDVVKDGGSGRCSKSKRRK- 663
           +VH EVVDA GVT VVA +D+S   R CGCW+ K RRRRAV V K+GGSGR SK K RK 
Sbjct: 601 DVHKEVVDATGVTDVVAGSDNSKCRRGCGCWNLKSRRRRAVAVDKEGGSGRRSKFKGRKG 640

BLAST of Bhi01G001263 vs. NCBI nr
Match: XP_022922695.1 (uncharacterized protein LOC111430613 isoform X4 [Cucurbita moschata])

HSP 1 Score: 401.0 bits (1029), Expect = 2.0e-107
Identity = 356/907 (39.25%), Postives = 412/907 (45.42%), Query Frame = 0

Query: 7   TTDKQMEEPKSRKPRQRRNLQMEELPTFTKWLNSVGHSGSCNDANSKSKPVNGNRPVVRT 66
           TTDKQ E+ K +K   RRNLQ+EE PTFTKWL   G S S +D +SKS       P VR 
Sbjct: 3   TTDKQKEDGKPKKKCHRRNLQIEEFPTFTKWLGPRGRSSSRDDTSSKSYKRGVPYPPVRR 62

Query: 67  PVVVFDSSEDGGAVVA-DVPK-DRVQAVDDSISARAGCCCWQSSKSTRRECALKFHLSLR 126
             +  DSS  GG VVA DV K D   A+ +SISAR GCCCWQ SKST+REC L FH SLR
Sbjct: 63  --IPRDSSSGGGGVVACDVSKEDGGVAIGESISARGGCCCWQRSKSTQRECGLNFHFSLR 122

Query: 127 KRKVVTNGSEVEVVTVVSNLREEETVVAVSDVLEKDGCGCRCFPTFQICGRRK------- 186
           K KVVTN SE  V   VS++REE+ VV    V+ ++GCGCRC PTF ICGRRK       
Sbjct: 123 KSKVVTNVSE-GVAAGVSDVREEQ-VVEAGAVINREGCGCRCSPTFHICGRRKVPPTGVS 182

Query: 187 -----------------------SIVVLQKEDGAVTDDPNLRTEEVANRGPDLVKEEEVV 246
                                      L KE+G  TD P++  EEVA   PDLVKE E +
Sbjct: 183 YLPEKRSGRRKSRSIRRRGLPVVDAPNLHKEEGPATDGPDVLKEEVATSCPDLVKEGEAI 242

Query: 247 VVGG-------------------------------------------------------- 306
              G                                                        
Sbjct: 243 AAPGPCKEGSRSCCSQWKCLPSFPMCGRKLSVVEEEVTIEEEVSVYDPNGPEVEEVANGP 302

Query: 307 -----------------------------------------------------------S 366
                                                                       
Sbjct: 303 ELVKEGEATVAPGPCKEESGYCFPQWKCLPSFPTCGRKLSVVEEEVTVDDPNVPEVANRP 362

Query: 367 DLRKE-----------EGSGCCCGRCGCFPAFQICRRRNVVASKEEAVVDVPEV--EEEV 426
           DL KE           EGSGCC  R      FQI R   V A K+E  VDVP V   EEV
Sbjct: 363 DLAKEGEATVAPDPRKEGSGCCSPRLKDVSEFQI-RNSKVAAGKQEMTVDVPNVLEVEEV 422

Query: 427 ANDKVNKQ-----EGDSVECLQAFQ--SHICCAG-------------------------R 486
           AND VNKQ     E   V  L  FQ  S    AG                         +
Sbjct: 423 ANDVVNKQDDICPEKQEVIGLPGFQICSSKVAAGKQEMTIDVPNVPEVEEVANDVVKKQK 482

Query: 487 KTVDDNPKTFEKEALAS--------EDSSNVDVPDLQKEGSGGCCSCFKCMPTLHICGRR 546
           K VD +P T E  A  S        E+  N  VP+L K GSG  C  FK MP+  ICG +
Sbjct: 483 KEVDGDPNTGEAVASCSSSSNLHEEEEKINASVPELHKNGSG--CGWFKWMPSFLICGSK 542

Query: 547 RNAVSEVPNPGREEKVVVSVSDPPEGEEVVA----------ADEEWHSKSTQGGICWPFQ 606
              VS+VPNP REEKVV   SD  EG    A          AD E  SKS + G CWPFQ
Sbjct: 543 --VVSDVPNPSREEKVVAGDSDIQEGVGAAAVAVAAAADSDADWEAQSKSARQGSCWPFQ 602

Query: 607 ICTRGWLPRFFLCGERITVDASNHREEEEKAPPDVQKEEVVAVIPDPQKESIAVADGIPD 663
            CT  W P   LC   + V+ASNH+EEE++ P    KE+VV V+     E    A G+PD
Sbjct: 603 CCTI-WWPSKLLCTGGMDVNASNHQEEEQEEPLSDNKEKVVDVLTVKHNEK-GAAMGVPD 662

BLAST of Bhi01G001263 vs. NCBI nr
Match: XP_022922694.1 (uncharacterized protein LOC111430613 isoform X3 [Cucurbita moschata])

HSP 1 Score: 368.2 bits (944), Expect = 1.5e-97
Identity = 345/980 (35.20%), Postives = 414/980 (42.24%), Query Frame = 0

Query: 7   TTDKQMEEPKSRKPRQRRNLQMEELPTFTKWLNSVGHSGSCNDANSKSKPVNGNRPVVRT 66
           TTDKQ E+ K +K   RRNLQ+EE PTFTKWL   G S S +D +SKS       P VR 
Sbjct: 3   TTDKQKEDGKPKKKCHRRNLQIEEFPTFTKWLGPRGRSSSRDDTSSKSYKRGVPYPPVRR 62

Query: 67  PVVVFDSSEDGGAVVA-DVPK-DRVQAVDDSISARAGCCCWQSSKSTRRECALKFHLSLR 126
             +  DSS  GG VVA DV K D   A+ +SISAR GCCCWQ SKST+REC L FH SLR
Sbjct: 63  --IPRDSSSGGGGVVACDVSKEDGGVAIGESISARGGCCCWQRSKSTQRECGLNFHFSLR 122

Query: 127 KRKVVTNGSEVEVVTVVSNLREEETVVAVSDVLEKDGCGCRCFPTFQICGRRK------- 186
           K KVVTN SE  V   VS++REE+ VV    V+ ++GCGCRC PTF ICGRRK       
Sbjct: 123 KSKVVTNVSE-GVAAGVSDVREEQ-VVEAGAVINREGCGCRCSPTFHICGRRKVPPTGVS 182

Query: 187 -----------------------SIVVLQKEDGAVTDDPNLRTEEVANRGPDLVKEEEVV 246
                                      L KE+G  TD P++  EEVA   PDLVKE E +
Sbjct: 183 YLPEKRSGRRKSRSIRRRGLPVVDAPNLHKEEGPATDGPDVLKEEVATSCPDLVKEGEAI 242

Query: 247 ------------------------------------------------------------ 306
                                                                       
Sbjct: 243 AAPGPCKEGSRSCCSQWKCLPSFPMCGRKLSVVEEEVTIEEEVSVYDPNGPEVEEVANGP 302

Query: 307 ---------------------------------------------------------VVG 366
                                                                    V  
Sbjct: 303 ESVKEGEATIAPAPCKEGSESCSQWKCSPSIPMCERKVATGEEELTVVNVPNLLDVEVAN 362

Query: 367 GSDLRKE----------EGSGCCCGRCGCFPAFQICRRRNVVASKEEAVVDVPEV--EEE 426
           G +L +E          + SG CC +  C PAF +C R+   +  E  VVD+P +   EE
Sbjct: 363 GPELVQEGEATDAPSLCKRSGSCCSQWKCLPAFPMCGRKVATSKVELTVVDIPNLLEVEE 422

Query: 427 VANDKVNKQEGDSV-----------------ECLQAF----------------------- 486
           VAN     +EG +                  +CL +F                       
Sbjct: 423 VANGPELVKEGVATGSPGPYKKRSGSCCSQWKCLPSFPTCGRKLSVVEEEVTVDDPNVPE 482

Query: 487 ---------------------QSHICCAGR------------------------------ 546
                                +   CC+ R                              
Sbjct: 483 VANRPDLAKEGEATVAPDPRKEGSGCCSPRLKDVSEFQIRNSKVAAGKQEMTVDVPNVLE 542

Query: 547 -------------KTVDDNPKTFEKEALAS--------EDSSNVDVPDLQKEGSGGCCSC 606
                        K VD +P T E  A  S        E+  N  VP+L K GSG  C  
Sbjct: 543 VEEVANDVVNKQKKEVDGDPNTGEAVASCSSSSNLHEEEEKINASVPELHKNGSG--CGW 602

Query: 607 FKCMPTLHICGRRRNAVSEVPNPGREEKVVVSVSDPPEGEEVVA----------ADEEWH 663
           FK MP+  ICG +   VS+VPNP REEKVV   SD  EG    A          AD E  
Sbjct: 603 FKWMPSFLICGSK--VVSDVPNPSREEKVVAGDSDIQEGVGAAAVAVAAAADSDADWEAQ 662

BLAST of Bhi01G001263 vs. NCBI nr
Match: XP_022922693.1 (uncharacterized protein LOC111430613 isoform X2 [Cucurbita moschata])

HSP 1 Score: 356.3 bits (913), Expect = 5.8e-94
Identity = 354/1031 (34.34%), Postives = 416/1031 (40.35%), Query Frame = 0

Query: 7    TTDKQMEEPKSRKPRQRRNLQMEELPTFTKWLNSVGHSGSCNDANSKSKPVNGNRPVVRT 66
            TTDKQ E+ K +K   RRNLQ+EE PTFTKWL   G S S +D +SKS       P VR 
Sbjct: 3    TTDKQKEDGKPKKKCHRRNLQIEEFPTFTKWLGPRGRSSSRDDTSSKSYKRGVPYPPVRR 62

Query: 67   PVVVFDSSEDGGAVVA-DVPK-DRVQAVDDSISARAGCCCWQSSKSTRRECALKFHLSLR 126
              +  DSS  GG VVA DV K D   A+ +SISAR GCCCWQ SKST+REC L FH SLR
Sbjct: 63   --IPRDSSSGGGGVVACDVSKEDGGVAIGESISARGGCCCWQRSKSTQRECGLNFHFSLR 122

Query: 127  KRKVVTNGSEVEVVTVVSNLREEETVVAVSDVLEKDGCGCRCFPTFQICGRRK------- 186
            K KVVTN SE  V   VS++REE+ VV    V+ ++GCGCRC PTF ICGRRK       
Sbjct: 123  KSKVVTNVSE-GVAAGVSDVREEQ-VVEAGAVINREGCGCRCSPTFHICGRRKVPPTGVS 182

Query: 187  -----------------------SIVVLQKEDGAVTDDPNLRTEEVANRGPDLVKEEEVV 246
                                       L KE+G  TD P++  EEVA   PDLVKE E +
Sbjct: 183  YLPEKRSGRRKSRSIRRRGLPVVDAPNLHKEEGPATDGPDVLKEEVATSCPDLVKEGEAI 242

Query: 247  ------------------------------------------------------------ 306
                                                                        
Sbjct: 243  AAPGPCKEGSRSCCSQWKCLPSFPMCGRKLSVVEEEVTIEEEVSVYDPNGPEVEEVANGP 302

Query: 307  ---------------------------------------------------------VVG 366
                                                                     V  
Sbjct: 303  ESVKEGEATIAPAPCKEGSESCSQWKCSPSIPMCERKVATGEEELTVVNVPNLLDVEVAN 362

Query: 367  GSDLRKE----------EGSGCCCGRCGCFPAFQICRRR--------------------- 426
            G +L +E          + SG CC +  C PAF +C R+                     
Sbjct: 363  GPELVQEGEATDAPSLCKRSGSCCSQWKCLPAFPMCGRKVATSKEELTDVDVPNLLEVEE 422

Query: 427  ------------------------------------------------------------ 486
                                                                        
Sbjct: 423  VANGPELVKEGEATVAPGPCKEESGYCFPQWKCLPSFPTCGRKLSVVEEEVTVDDPNVPE 482

Query: 487  ------------------------------------------NVVASKEEAVVDVPEV-- 546
                                                       V A K+E  VDVP V  
Sbjct: 483  VANRPDLAKEGEATVAPDPRKEGSGCCSPRLKDVSEFQIRNSKVAAGKQEMTVDVPNVLE 542

Query: 547  EEEVANDKVNKQ-----EGDSVECLQAFQ--SHICCAG---------------------- 606
             EEVAND VNKQ     E   V  L  FQ  S    AG                      
Sbjct: 543  VEEVANDVVNKQDDICPEKQEVIGLPGFQICSSKVAAGKQEMTIDVPNVPEVEEVANDVV 602

Query: 607  ---RKTVDDNPKTFEKEALAS--------EDSSNVDVPDLQKEGSGGCCSCFKCMPTLHI 663
               +K VD +P T E  A  S        E+  N  VP+L K GSG  C  FK MP+  I
Sbjct: 603  KKQKKEVDGDPNTGEAVASCSSSSNLHEEEEKINASVPELHKNGSG--CGWFKWMPSFLI 662

BLAST of Bhi01G001263 vs. ExPASy TrEMBL
Match: A0A0A0L996 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G204780 PE=4 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 2.9e-192
Identity = 416/680 (61.18%), Postives = 465/680 (68.38%), Query Frame = 0

Query: 6   MTTDKQMEEPKSRKPRQRRNLQMEELPTFTKWLNSVGHSGSCNDANSKSKPVN-GNRPVV 65
           M T+K  EE +SRKPR RRN QMEE PTFTKWL + GHSGSCNDA SKSK +N  NRP+ 
Sbjct: 1   MATNKPKEEQRSRKPRHRRNQQMEEFPTFTKWLTTFGHSGSCNDAKSKSKQLNPANRPLQ 60

Query: 66  RTPVVVFDSSEDGGAVVADVPKD---RVQAVDDSISAR--AGCCCWQSSKSTRRECALKF 125
           R PVV+  SSED   VV +VP++   +  AVD SISAR  AGCCCWQSSKSTRRECALKF
Sbjct: 61  RPPVVLPASSEDAVPVVTNVPEEEGVQTMAVDGSISARGGAGCCCWQSSKSTRRECALKF 120

Query: 126 HLSLRKRKVVTNGSEVEVVTVVSNLREEETVVAVSDVLEKDGCGCRCFPTFQICGRRKSI 185
           H+SLRKRKVV N +E EVV  V N  EE TV  V     KDGCGCRC  TF+I  RRKS 
Sbjct: 121 HISLRKRKVVANSTEAEVVAAVLNPPEEATVEDV-----KDGCGCRCLRTFKIFRRRKSR 180

Query: 186 VV----LQKEDGAVTDDPNLRTEEVANRGPDLVKEEEVVVVGGSDLRKEEGSGCCCGRCG 245
           VV    LQKE+GAVTD  NLRTEEVA+ G D++KEEEVV+    D RKEE SGCCCGR  
Sbjct: 181 VVGVSDLQKEEGAVTDGVNLRTEEVASSGSDMMKEEEVVI--APDRRKEEESGCCCGRWK 240

Query: 246 CFPAFQICRRRNVVASKEEAVVDVPEVEEEVANDKVNKQEGDSVECLQAFQSHICCAGRK 305
           C P FQICRRR VVA KEE V   P+V EEV ND V KQE DSV CLQAF  HI C GRK
Sbjct: 241 CSPTFQICRRRKVVAGKEEVVGGAPKV-EEVGNDNVTKQEEDSVGCLQAF--HI-CGGRK 300

Query: 306 TVDDNPKTFEKEALASEDSSNVDVPDLQKEGSGGCCSCFKCMPTLHIC-GRRRNAVSEVP 365
            VDDNPKT EKE L S DSSN+DV +LQKE S GCCSCF+C+PT  IC GRR N  S VP
Sbjct: 301 RVDDNPKTSEKEPLVSNDSSNLDVQNLQKEES-GCCSCFRCIPTFQICGGRRSNEDSGVP 360

Query: 366 NPGREEKVVVSVSDPPEGEEVVAADEEWHSKSTQGGICWPFQICTRGWLPRFFLCGERIT 425
            PGREEKV+V VSDPPE   VV   E  HS+  QGG CW       GW PRF LCGE   
Sbjct: 361 KPGREEKVIVDVSDPPEMGSVVDGRER-HSRPVQGGTCW------SGWFPRFLLCGEGTA 420

Query: 426 VDASNHREEEEKAPPDVQKEEVVAVIPDPQKESIAVADGIPDDGKEKQVAADDIPVQSTE 485
           VDA NHREEEEKAP D +KEE V V       + AV D I D  KEK VAA DIPV + E
Sbjct: 421 VDAPNHREEEEKAPSDARKEEKVVV-------ATAVGDEISDHDKEKPVAAIDIPVVNEE 480

Query: 486 E-------KMSAGKEENASSGSIQETFEPDLNQEEIVDSGVSDLNQEDEGGCCRCFKLGG 545
           E        +   KE+N SS +IQ     D+ +EEIVDS     +++ EGG C C+   G
Sbjct: 481 EVFVGAGDTLDLHKEKNVSSCNIQ-----DVRKEEIVDS-----DEKVEGGGCGCW---G 540

Query: 546 KEGSRRQHRRSPKSREGGWGFQIC--GWLPTLDMCRGRKKESVSIAKLHEKEGLVDNGVS 605
           KE   RQ  RS +S EG W FQIC  G LPTL++CRGRK  SV I+KL E EGLVDN VS
Sbjct: 541 KESGSRQQHRSSRSMEGCWSFQICGRGCLPTLNICRGRKDVSVRISKLVE-EGLVDNDVS 600

Query: 606 EVHNEVVDAAGVTGVVADTDHSNSTRCCGCWHSKPRRRRAVDVVKDGGSGRCSKSKRRK- 663
           +VH EVVDA GVT VVA +D+S   R CGCW+ K RRRRAV V K+GGSGR SK K RK 
Sbjct: 601 DVHKEVVDATGVTDVVAGSDNSKCRRGCGCWNLKSRRRRAVAVDKEGGSGRRSKFKGRKG 640

BLAST of Bhi01G001263 vs. ExPASy TrEMBL
Match: A0A6J1E9I0 (uncharacterized protein LOC111430613 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111430613 PE=4 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 9.9e-108
Identity = 356/907 (39.25%), Postives = 412/907 (45.42%), Query Frame = 0

Query: 7   TTDKQMEEPKSRKPRQRRNLQMEELPTFTKWLNSVGHSGSCNDANSKSKPVNGNRPVVRT 66
           TTDKQ E+ K +K   RRNLQ+EE PTFTKWL   G S S +D +SKS       P VR 
Sbjct: 3   TTDKQKEDGKPKKKCHRRNLQIEEFPTFTKWLGPRGRSSSRDDTSSKSYKRGVPYPPVRR 62

Query: 67  PVVVFDSSEDGGAVVA-DVPK-DRVQAVDDSISARAGCCCWQSSKSTRRECALKFHLSLR 126
             +  DSS  GG VVA DV K D   A+ +SISAR GCCCWQ SKST+REC L FH SLR
Sbjct: 63  --IPRDSSSGGGGVVACDVSKEDGGVAIGESISARGGCCCWQRSKSTQRECGLNFHFSLR 122

Query: 127 KRKVVTNGSEVEVVTVVSNLREEETVVAVSDVLEKDGCGCRCFPTFQICGRRK------- 186
           K KVVTN SE  V   VS++REE+ VV    V+ ++GCGCRC PTF ICGRRK       
Sbjct: 123 KSKVVTNVSE-GVAAGVSDVREEQ-VVEAGAVINREGCGCRCSPTFHICGRRKVPPTGVS 182

Query: 187 -----------------------SIVVLQKEDGAVTDDPNLRTEEVANRGPDLVKEEEVV 246
                                      L KE+G  TD P++  EEVA   PDLVKE E +
Sbjct: 183 YLPEKRSGRRKSRSIRRRGLPVVDAPNLHKEEGPATDGPDVLKEEVATSCPDLVKEGEAI 242

Query: 247 VVGG-------------------------------------------------------- 306
              G                                                        
Sbjct: 243 AAPGPCKEGSRSCCSQWKCLPSFPMCGRKLSVVEEEVTIEEEVSVYDPNGPEVEEVANGP 302

Query: 307 -----------------------------------------------------------S 366
                                                                       
Sbjct: 303 ELVKEGEATVAPGPCKEESGYCFPQWKCLPSFPTCGRKLSVVEEEVTVDDPNVPEVANRP 362

Query: 367 DLRKE-----------EGSGCCCGRCGCFPAFQICRRRNVVASKEEAVVDVPEV--EEEV 426
           DL KE           EGSGCC  R      FQI R   V A K+E  VDVP V   EEV
Sbjct: 363 DLAKEGEATVAPDPRKEGSGCCSPRLKDVSEFQI-RNSKVAAGKQEMTVDVPNVLEVEEV 422

Query: 427 ANDKVNKQ-----EGDSVECLQAFQ--SHICCAG-------------------------R 486
           AND VNKQ     E   V  L  FQ  S    AG                         +
Sbjct: 423 ANDVVNKQDDICPEKQEVIGLPGFQICSSKVAAGKQEMTIDVPNVPEVEEVANDVVKKQK 482

Query: 487 KTVDDNPKTFEKEALAS--------EDSSNVDVPDLQKEGSGGCCSCFKCMPTLHICGRR 546
           K VD +P T E  A  S        E+  N  VP+L K GSG  C  FK MP+  ICG +
Sbjct: 483 KEVDGDPNTGEAVASCSSSSNLHEEEEKINASVPELHKNGSG--CGWFKWMPSFLICGSK 542

Query: 547 RNAVSEVPNPGREEKVVVSVSDPPEGEEVVA----------ADEEWHSKSTQGGICWPFQ 606
              VS+VPNP REEKVV   SD  EG    A          AD E  SKS + G CWPFQ
Sbjct: 543 --VVSDVPNPSREEKVVAGDSDIQEGVGAAAVAVAAAADSDADWEAQSKSARQGSCWPFQ 602

Query: 607 ICTRGWLPRFFLCGERITVDASNHREEEEKAPPDVQKEEVVAVIPDPQKESIAVADGIPD 663
            CT  W P   LC   + V+ASNH+EEE++ P    KE+VV V+     E    A G+PD
Sbjct: 603 CCTI-WWPSKLLCTGGMDVNASNHQEEEQEEPLSDNKEKVVDVLTVKHNEK-GAAMGVPD 662

BLAST of Bhi01G001263 vs. ExPASy TrEMBL
Match: A0A6J1E452 (uncharacterized protein LOC111430613 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111430613 PE=4 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 7.1e-98
Identity = 345/980 (35.20%), Postives = 414/980 (42.24%), Query Frame = 0

Query: 7   TTDKQMEEPKSRKPRQRRNLQMEELPTFTKWLNSVGHSGSCNDANSKSKPVNGNRPVVRT 66
           TTDKQ E+ K +K   RRNLQ+EE PTFTKWL   G S S +D +SKS       P VR 
Sbjct: 3   TTDKQKEDGKPKKKCHRRNLQIEEFPTFTKWLGPRGRSSSRDDTSSKSYKRGVPYPPVRR 62

Query: 67  PVVVFDSSEDGGAVVA-DVPK-DRVQAVDDSISARAGCCCWQSSKSTRRECALKFHLSLR 126
             +  DSS  GG VVA DV K D   A+ +SISAR GCCCWQ SKST+REC L FH SLR
Sbjct: 63  --IPRDSSSGGGGVVACDVSKEDGGVAIGESISARGGCCCWQRSKSTQRECGLNFHFSLR 122

Query: 127 KRKVVTNGSEVEVVTVVSNLREEETVVAVSDVLEKDGCGCRCFPTFQICGRRK------- 186
           K KVVTN SE  V   VS++REE+ VV    V+ ++GCGCRC PTF ICGRRK       
Sbjct: 123 KSKVVTNVSE-GVAAGVSDVREEQ-VVEAGAVINREGCGCRCSPTFHICGRRKVPPTGVS 182

Query: 187 -----------------------SIVVLQKEDGAVTDDPNLRTEEVANRGPDLVKEEEVV 246
                                      L KE+G  TD P++  EEVA   PDLVKE E +
Sbjct: 183 YLPEKRSGRRKSRSIRRRGLPVVDAPNLHKEEGPATDGPDVLKEEVATSCPDLVKEGEAI 242

Query: 247 ------------------------------------------------------------ 306
                                                                       
Sbjct: 243 AAPGPCKEGSRSCCSQWKCLPSFPMCGRKLSVVEEEVTIEEEVSVYDPNGPEVEEVANGP 302

Query: 307 ---------------------------------------------------------VVG 366
                                                                    V  
Sbjct: 303 ESVKEGEATIAPAPCKEGSESCSQWKCSPSIPMCERKVATGEEELTVVNVPNLLDVEVAN 362

Query: 367 GSDLRKE----------EGSGCCCGRCGCFPAFQICRRRNVVASKEEAVVDVPEV--EEE 426
           G +L +E          + SG CC +  C PAF +C R+   +  E  VVD+P +   EE
Sbjct: 363 GPELVQEGEATDAPSLCKRSGSCCSQWKCLPAFPMCGRKVATSKVELTVVDIPNLLEVEE 422

Query: 427 VANDKVNKQEGDSV-----------------ECLQAF----------------------- 486
           VAN     +EG +                  +CL +F                       
Sbjct: 423 VANGPELVKEGVATGSPGPYKKRSGSCCSQWKCLPSFPTCGRKLSVVEEEVTVDDPNVPE 482

Query: 487 ---------------------QSHICCAGR------------------------------ 546
                                +   CC+ R                              
Sbjct: 483 VANRPDLAKEGEATVAPDPRKEGSGCCSPRLKDVSEFQIRNSKVAAGKQEMTVDVPNVLE 542

Query: 547 -------------KTVDDNPKTFEKEALAS--------EDSSNVDVPDLQKEGSGGCCSC 606
                        K VD +P T E  A  S        E+  N  VP+L K GSG  C  
Sbjct: 543 VEEVANDVVNKQKKEVDGDPNTGEAVASCSSSSNLHEEEEKINASVPELHKNGSG--CGW 602

Query: 607 FKCMPTLHICGRRRNAVSEVPNPGREEKVVVSVSDPPEGEEVVA----------ADEEWH 663
           FK MP+  ICG +   VS+VPNP REEKVV   SD  EG    A          AD E  
Sbjct: 603 FKWMPSFLICGSK--VVSDVPNPSREEKVVAGDSDIQEGVGAAAVAVAAAADSDADWEAQ 662

BLAST of Bhi01G001263 vs. ExPASy TrEMBL
Match: A0A6J1E7K5 (uncharacterized protein LOC111430613 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430613 PE=4 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 2.8e-94
Identity = 354/1031 (34.34%), Postives = 416/1031 (40.35%), Query Frame = 0

Query: 7    TTDKQMEEPKSRKPRQRRNLQMEELPTFTKWLNSVGHSGSCNDANSKSKPVNGNRPVVRT 66
            TTDKQ E+ K +K   RRNLQ+EE PTFTKWL   G S S +D +SKS       P VR 
Sbjct: 3    TTDKQKEDGKPKKKCHRRNLQIEEFPTFTKWLGPRGRSSSRDDTSSKSYKRGVPYPPVRR 62

Query: 67   PVVVFDSSEDGGAVVA-DVPK-DRVQAVDDSISARAGCCCWQSSKSTRRECALKFHLSLR 126
              +  DSS  GG VVA DV K D   A+ +SISAR GCCCWQ SKST+REC L FH SLR
Sbjct: 63   --IPRDSSSGGGGVVACDVSKEDGGVAIGESISARGGCCCWQRSKSTQRECGLNFHFSLR 122

Query: 127  KRKVVTNGSEVEVVTVVSNLREEETVVAVSDVLEKDGCGCRCFPTFQICGRRK------- 186
            K KVVTN SE  V   VS++REE+ VV    V+ ++GCGCRC PTF ICGRRK       
Sbjct: 123  KSKVVTNVSE-GVAAGVSDVREEQ-VVEAGAVINREGCGCRCSPTFHICGRRKVPPTGVS 182

Query: 187  -----------------------SIVVLQKEDGAVTDDPNLRTEEVANRGPDLVKEEEVV 246
                                       L KE+G  TD P++  EEVA   PDLVKE E +
Sbjct: 183  YLPEKRSGRRKSRSIRRRGLPVVDAPNLHKEEGPATDGPDVLKEEVATSCPDLVKEGEAI 242

Query: 247  ------------------------------------------------------------ 306
                                                                        
Sbjct: 243  AAPGPCKEGSRSCCSQWKCLPSFPMCGRKLSVVEEEVTIEEEVSVYDPNGPEVEEVANGP 302

Query: 307  ---------------------------------------------------------VVG 366
                                                                     V  
Sbjct: 303  ESVKEGEATIAPAPCKEGSESCSQWKCSPSIPMCERKVATGEEELTVVNVPNLLDVEVAN 362

Query: 367  GSDLRKE----------EGSGCCCGRCGCFPAFQICRRR--------------------- 426
            G +L +E          + SG CC +  C PAF +C R+                     
Sbjct: 363  GPELVQEGEATDAPSLCKRSGSCCSQWKCLPAFPMCGRKVATSKEELTDVDVPNLLEVEE 422

Query: 427  ------------------------------------------------------------ 486
                                                                        
Sbjct: 423  VANGPELVKEGEATVAPGPCKEESGYCFPQWKCLPSFPTCGRKLSVVEEEVTVDDPNVPE 482

Query: 487  ------------------------------------------NVVASKEEAVVDVPEV-- 546
                                                       V A K+E  VDVP V  
Sbjct: 483  VANRPDLAKEGEATVAPDPRKEGSGCCSPRLKDVSEFQIRNSKVAAGKQEMTVDVPNVLE 542

Query: 547  EEEVANDKVNKQ-----EGDSVECLQAFQ--SHICCAG---------------------- 606
             EEVAND VNKQ     E   V  L  FQ  S    AG                      
Sbjct: 543  VEEVANDVVNKQDDICPEKQEVIGLPGFQICSSKVAAGKQEMTIDVPNVPEVEEVANDVV 602

Query: 607  ---RKTVDDNPKTFEKEALAS--------EDSSNVDVPDLQKEGSGGCCSCFKCMPTLHI 663
               +K VD +P T E  A  S        E+  N  VP+L K GSG  C  FK MP+  I
Sbjct: 603  KKQKKEVDGDPNTGEAVASCSSSSNLHEEEEKINASVPELHKNGSG--CGWFKWMPSFLI 662

BLAST of Bhi01G001263 vs. ExPASy TrEMBL
Match: A0A6J1E412 (uncharacterized protein LOC111430613 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430613 PE=4 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 2.8e-94
Identity = 354/1031 (34.34%), Postives = 416/1031 (40.35%), Query Frame = 0

Query: 7    TTDKQMEEPKSRKPRQRRNLQMEELPTFTKWLNSVGHSGSCNDANSKSKPVNGNRPVVRT 66
            TTDKQ E+ K +K   RRNLQ+EE PTFTKWL   G S S +D +SKS       P VR 
Sbjct: 3    TTDKQKEDGKPKKKCHRRNLQIEEFPTFTKWLGPRGRSSSRDDTSSKSYKRGVPYPPVRR 62

Query: 67   PVVVFDSSEDGGAVVA-DVPK-DRVQAVDDSISARAGCCCWQSSKSTRRECALKFHLSLR 126
              +  DSS  GG VVA DV K D   A+ +SISAR GCCCWQ SKST+REC L FH SLR
Sbjct: 63   --IPRDSSSGGGGVVACDVSKEDGGVAIGESISARGGCCCWQRSKSTQRECGLNFHFSLR 122

Query: 127  KRKVVTNGSEVEVVTVVSNLREEETVVAVSDVLEKDGCGCRCFPTFQICGRRK------- 186
            K KVVTN SE  V   VS++REE+ VV    V+ ++GCGCRC PTF ICGRRK       
Sbjct: 123  KSKVVTNVSE-GVAAGVSDVREEQ-VVEAGAVINREGCGCRCSPTFHICGRRKVPPTGVS 182

Query: 187  -----------------------SIVVLQKEDGAVTDDPNLRTEEVANRGPDLVKEEEVV 246
                                       L KE+G  TD P++  EEVA   PDLVKE E +
Sbjct: 183  YLPEKRSGRRKSRSIRRRGLPVVDAPNLHKEEGPATDGPDVLKEEVATSCPDLVKEGEAI 242

Query: 247  ------------------------------------------------------------ 306
                                                                        
Sbjct: 243  AAPGPCKEGSRSCCSQWKCLPSFPMCGRKLSVVEEEVTIEEEVSVYDPNGPEVEEVANGP 302

Query: 307  ---------------------------------------------------------VVG 366
                                                                     V  
Sbjct: 303  ESVKEGEATIAPAPCKEGSESCSQWKCSPSIPMCERKVATGEEELTVVNVPNLLDVEVAN 362

Query: 367  GSDLRKE----------EGSGCCCGRCGCFPAFQICRRR--------------------- 426
            G +L +E          + SG CC +  C PAF +C R+                     
Sbjct: 363  GPELVQEGEATDAPSLCKRSGSCCSQWKCLPAFPMCGRKVATSKVELTVVDIPNLLEVEE 422

Query: 427  ------------------------------------------------------------ 486
                                                                        
Sbjct: 423  VANGPELVKEGVATGSPGPYKKRSGSCCSQWKCLPSFPTCGRKLSVVEEEVTVDDPNVPE 482

Query: 487  ------------------------------------------NVVASKEEAVVDVPEV-- 546
                                                       V A K+E  VDVP V  
Sbjct: 483  VANRPDLAKEGEATVAPDPRKEGSGCCSPRLKDVSEFQIRNSKVAAGKQEMTVDVPNVLE 542

Query: 547  EEEVANDKVNKQ-----EGDSVECLQAFQ--SHICCAG---------------------- 606
             EEVAND VNKQ     E   V  L  FQ  S    AG                      
Sbjct: 543  VEEVANDVVNKQDDICPEKQEVIGLPGFQICSSKVAAGKQEMTIDVPNVPEVEEVANDVV 602

Query: 607  ---RKTVDDNPKTFEKEALAS--------EDSSNVDVPDLQKEGSGGCCSCFKCMPTLHI 663
               +K VD +P T E  A  S        E+  N  VP+L K GSG  C  FK MP+  I
Sbjct: 603  KKQKKEVDGDPNTGEAVASCSSSSNLHEEEEKINASVPELHKNGSG--CGWFKWMPSFLI 662