Bhi01G000499 (gene) Wax gourd (B227) v1

Overview
NameBhi01G000499
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionProtein NRT1/ PTR FAMILY 5.2-like
Locationchr1: 12887459 .. 12889379 (-)
RNA-Seq ExpressionBhi01G000499
SyntenyBhi01G000499
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAATGTCTCTTCTAACACTGGCAGTGTCCATGCCAAGCCTAAAGCCCCATCCATGTTTGGATGCAAACGCAGAACATTGCAAGCAGACCTCCAGGCTACAACTCGCTGTCTTCTTCACTGCACTCTACATATTGGCTCTTGGCACGGGTGGAACCAAACCAAACATCTCGACAATCGGAGCCGACCAGTTCGACGACTTCCATCCCGAAGAGAAGGCCCAGAAGTTATCCTTCTTCAATTGGTGGATGTTCAGCATTTTCTTTGGCACTCTCTTTGCCACCACCACTCTTGTTTACATACAAGAGAATGTAGGATGGAGCTTGGGATATGGCATTCCCACTGTAGGCCTTGCAATAGCCATTCTCATATTTGCTGCAGGCACACCTTTTTACAGGCACAAGCTCCCCACTGGAAGCCCTTTCATCAGGATGGCTAAGGTCATTGTAGCTACTGCTTGGAATTGGAGGCAGCCTCTTCCTTATGACCCTAGGCAACTCCATGAGCTTCAGCTTGAGGAGTACACTAAGCAAGGAACTTTCAGGATTGATTCCACTCCATCCTTGAGGTTACTATCTCTAAAATTAGAATCACTTGCATTAGCTGCTAAATTATTGTTGTTACATACCTGTTAACATCACCGTGTTCGTTTGTGTTCAGATCCAAACATAGTCACACAATGATCAAAAGTAGGAAAGATACATTGACACGAGCTATTTTAGGTGAAATCAAAAGAAAAGTACAGAGATTTATATGCAAAGTGAATAAAAAGCCCAATTTGTATAAATCAGAGTAGTTTTATTTAGTTAGAACTTGGTTCTGAAGTGTTTATGGATGTAGAACATCTTGGGATTACTGAATTGGCATAGGTGCAATATGGGGGGTTCAATGAGATTGTATTTCTTCACTGCATATGCAGGTTCCTCAACAAGGCTGCCATAAGAACAGGCTCAACTCATCCATGGAAGCTATGTTCAGTGACTCAAGTGGAGGAGACAAAACAGATGTTGAGAATGATCCCAATTCTGATATGCACATTCATTCCAAGCACAATGTTGTCACAGGCACAGACCCTTTTCATCAAGCAAGGCAGGACCCTCGACCGAAGCGTCGGTAGCCACTTTCAAATCCCTCCTGCAAGTTTAACTGCTTTTGTTACCATCTCCATGCTAGTCAGTGTCATCATCTACGACAGGTTTGCTAAAAAAATTATTGCCAATGCTACAAGTCATACGACTTCTTGTGTTACCATTAACCTGGTGTCTTCTTTCTTGAAGGTTCTTCGTCAAGATAATGCAGAGATTAACTAACAATCCAAGAGGAATCACAATGTTACAAAGATTGGGAATTGGGATGATTCTCCATATCTTGATAATGACAATTGCATCTTTGGTTGAAAGGCATAGACTCCATGTTGCTAGAAAAAATGGGTTGGAAAAAAATGAAGAACAACTCCCCCTGACCATTTTCACTCTCCTTCCTCAGTTCATGCTTGTGGGAGTTGCTGATGCATTTTCAGAAGTAGCAAAGATTGAATTCTTTTATGATCAAGCACCAGAAAGCATGAAGAGTTTAGGCACTTCGTATTCGATGACTTCGCTTGGAATTGGAAACTTCCTAAGTAGTTTTCTTCTTTCAACAGTTTCTCATCTTACAAAGAAACATGGCAATGGATGGATCAACAACAACTTGAATGCTTCTCATCTTGACTACTACTATGGGTTTTTGGCAGTGCTTAGTACCATCAACTTCTTCCTCTTCTTGCTCATGTCCAAAATGTACGTGTACAAAGCAGAAGTATCTGATTCAATAAAGGTGCTGACTGATAAACTCAAGGCATCCAACCTAAACGAAAAATCAGCTATGACGAGGGGCAGAGCCGATGAAGGAAGGGGGCCCTTGCCACCCTTCAATTTGTTTTAG

mRNA sequence

GGAATGTCTCTTCTAACACTGGCAGTGTCCATGCCAAGCCTAAAGCCCCATCCATGTTTGGATGCAAACGCAGAACATTGCAAGCAGACCTCCAGGCTACAACTCGCTGTCTTCTTCACTGCACTCTACATATTGGCTCTTGGCACGGGTGGAACCAAACCAAACATCTCGACAATCGGAGCCGACCAGTTCGACGACTTCCATCCCGAAGAGAAGGCCCAGAAGTTATCCTTCTTCAATTGGTGGATGTTCAGCATTTTCTTTGGCACTCTCTTTGCCACCACCACTCTTGTTTACATACAAGAGAATGTAGGATGGAGCTTGGGATATGGCATTCCCACTGTAGGCCTTGCAATAGCCATTCTCATATTTGCTGCAGGCACACCTTTTTACAGGCACAAGCTCCCCACTGGAAGCCCTTTCATCAGGATGGCTAAGGTCATTGTAGCTACTGCTTGGAATTGGAGGCAGCCTCTTCCTTATGACCCTAGGCAACTCCATGAGCTTCAGCTTGAGGAGTACACTAAGCAAGGAACTTTCAGGATTGATTCCACTCCATCCTTGAGGTTCCTCAACAAGGCTGCCATAAGAACAGGCTCAACTCATCCATGGAAGCTATGTTCAGTGACTCAAGTGGAGGAGACAAAACAGATGTTGAGAATGATCCCAATTCTGATATGCACATTCATTCCAAGCACAATGTTGTCACAGGCACAGACCCTTTTCATCAAGCAAGGCAGGACCCTCGACCGAAGCGTCGGTAGCCACTTTCAAATCCCTCCTGCAAGTTTAACTGCTTTTGTTACCATCTCCATGCTAGTCAGTGTCATCATCTACGACAGGTTCTTCGTCAAGATAATGCAGAGATTAACTAACAATCCAAGAGGAATCACAATGTTACAAAGATTGGGAATTGGGATGATTCTCCATATCTTGATAATGACAATTGCATCTTTGGTTGAAAGGCATAGACTCCATGTTGCTAGAAAAAATGGGTTGGAAAAAAATGAAGAACAACTCCCCCTGACCATTTTCACTCTCCTTCCTCAGTTCATGCTTGTGGGAGTTGCTGATGCATTTTCAGAAGTAGCAAAGATTGAATTCTTTTATGATCAAGCACCAGAAAGCATGAAGAGTTTAGGCACTTCGTATTCGATGACTTCGCTTGGAATTGGAAACTTCCTAAGTAGTTTTCTTCTTTCAACAGTTTCTCATCTTACAAAGAAACATGGCAATGGATGGATCAACAACAACTTGAATGCTTCTCATCTTGACTACTACTATGGGTTTTTGGCAGTGCTTAGTACCATCAACTTCTTCCTCTTCTTGCTCATGTCCAAAATGTACGTGTACAAAGCAGAAGTATCTGATTCAATAAAGGTGCTGACTGATAAACTCAAGGCATCCAACCTAAACGAAAAATCAGCTATGACGAGGGGCAGAGCCGATGAAGGAAGGGGGCCCTTGCCACCCTTCAATTTGTTTTAG

Coding sequence (CDS)

GGAATGTCTCTTCTAACACTGGCAGTGTCCATGCCAAGCCTAAAGCCCCATCCATGTTTGGATGCAAACGCAGAACATTGCAAGCAGACCTCCAGGCTACAACTCGCTGTCTTCTTCACTGCACTCTACATATTGGCTCTTGGCACGGGTGGAACCAAACCAAACATCTCGACAATCGGAGCCGACCAGTTCGACGACTTCCATCCCGAAGAGAAGGCCCAGAAGTTATCCTTCTTCAATTGGTGGATGTTCAGCATTTTCTTTGGCACTCTCTTTGCCACCACCACTCTTGTTTACATACAAGAGAATGTAGGATGGAGCTTGGGATATGGCATTCCCACTGTAGGCCTTGCAATAGCCATTCTCATATTTGCTGCAGGCACACCTTTTTACAGGCACAAGCTCCCCACTGGAAGCCCTTTCATCAGGATGGCTAAGGTCATTGTAGCTACTGCTTGGAATTGGAGGCAGCCTCTTCCTTATGACCCTAGGCAACTCCATGAGCTTCAGCTTGAGGAGTACACTAAGCAAGGAACTTTCAGGATTGATTCCACTCCATCCTTGAGGTTCCTCAACAAGGCTGCCATAAGAACAGGCTCAACTCATCCATGGAAGCTATGTTCAGTGACTCAAGTGGAGGAGACAAAACAGATGTTGAGAATGATCCCAATTCTGATATGCACATTCATTCCAAGCACAATGTTGTCACAGGCACAGACCCTTTTCATCAAGCAAGGCAGGACCCTCGACCGAAGCGTCGGTAGCCACTTTCAAATCCCTCCTGCAAGTTTAACTGCTTTTGTTACCATCTCCATGCTAGTCAGTGTCATCATCTACGACAGGTTCTTCGTCAAGATAATGCAGAGATTAACTAACAATCCAAGAGGAATCACAATGTTACAAAGATTGGGAATTGGGATGATTCTCCATATCTTGATAATGACAATTGCATCTTTGGTTGAAAGGCATAGACTCCATGTTGCTAGAAAAAATGGGTTGGAAAAAAATGAAGAACAACTCCCCCTGACCATTTTCACTCTCCTTCCTCAGTTCATGCTTGTGGGAGTTGCTGATGCATTTTCAGAAGTAGCAAAGATTGAATTCTTTTATGATCAAGCACCAGAAAGCATGAAGAGTTTAGGCACTTCGTATTCGATGACTTCGCTTGGAATTGGAAACTTCCTAAGTAGTTTTCTTCTTTCAACAGTTTCTCATCTTACAAAGAAACATGGCAATGGATGGATCAACAACAACTTGAATGCTTCTCATCTTGACTACTACTATGGGTTTTTGGCAGTGCTTAGTACCATCAACTTCTTCCTCTTCTTGCTCATGTCCAAAATGTACGTGTACAAAGCAGAAGTATCTGATTCAATAAAGGTGCTGACTGATAAACTCAAGGCATCCAACCTAAACGAAAAATCAGCTATGACGAGGGGCAGAGCCGATGAAGGAAGGGGGCCCTTGCCACCCTTCAATTTGTTTTAG

Protein sequence

GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIGADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIAILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTFRIDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQTLFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITMLQRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAFSEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGNGWINNNLNASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTDKLKASNLNEKSAMTRGRADEGRGPLPPFNLF
Homology
BLAST of Bhi01G000499 vs. TAIR 10
Match: AT5G46050.1 (peptide transporter 3 )

HSP 1 Score: 623.2 bits (1606), Expect = 1.7e-178
Identity = 308/468 (65.81%), Postives = 377/468 (80.56%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GM +LTL+V++P +KP  C   N E+C++ S LQLAVFF ALY LA+GTGGTKPNISTIG
Sbjct: 112 GMMVLTLSVTIPGIKPPECSTTNVENCEKASVLQLAVFFGALYTLAIGTGGTKPNISTIG 171

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFD F P+EK QKLSFFNWWMFSIFFGTLFA T LVY+Q+NVGW+LGYG+PT+GLAI+
Sbjct: 172 ADQFDVFDPKEKTQKLSFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAIS 231

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           I IF  GTPFYRHKLPTGSPF +MA+VIVA+      P+ +D    HEL   EY ++G F
Sbjct: 232 ITIFLLGTPFYRHKLPTGSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAF 291

Query: 181 RIDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQT 240
            I  TPSLRFL++A+++TG+ H W LC+ T+VEETKQMLRM+P+L  TF+PS ML+Q  T
Sbjct: 292 PIHPTPSLRFLDRASLKTGTNHKWNLCTTTEVEETKQMLRMLPVLFITFVPSMMLAQINT 351

Query: 241 LFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITML 300
           LF+KQG TLDR V   F IPPASL+ FVT+SML+S+++YDR FVKI ++ T NPRGIT+L
Sbjct: 352 LFVKQGTTLDRKVTGSFSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLL 411

Query: 301 QRLGIGMILHILIMTIASLVERHRLHVARKNGL-EKNEEQLPLTIFTLLPQFMLVGVADA 360
           QR+GIG+I HILIM +AS+ ER+RL VA  +GL  +   +LPLTIF LLPQF+L+G+AD+
Sbjct: 412 QRMGIGLIFHILIMIVASVTERYRLKVAADHGLIHQTGVKLPLTIFALLPQFVLMGMADS 471

Query: 361 FSEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGNGWINNNL 420
           F EVAK+EFFYDQAPESMKSLGTSYS TSL IGNF+SSFLLSTVS +TKK G GWI NNL
Sbjct: 472 FLEVAKLEFFYDQAPESMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKRGRGWILNNL 531

Query: 421 NASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTDKLK 468
           N S LDYYY F AVL+ +NF LFL++ K YVY+AEV+DS+ V   ++K
Sbjct: 532 NESRLDYYYLFFAVLNLVNFVLFLVVVKFYVYRAEVTDSVDVKEVEMK 579

BLAST of Bhi01G000499 vs. TAIR 10
Match: AT5G46040.1 (Major facilitator superfamily protein )

HSP 1 Score: 597.0 bits (1538), Expect = 1.3e-170
Identity = 294/462 (63.64%), Postives = 369/462 (79.87%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GM+LLTL+VS+P LKP  C  AN E+C++ S +QLAVFF ALY LA+GTGGTKPNISTIG
Sbjct: 112 GMALLTLSVSLPGLKPPKCSTANVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIG 171

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFD+F P++K  K SFFNWWMFSIFFGT FATT LVY+Q+NVGW++GYG+ T+GLA +
Sbjct: 172 ADQFDEFDPKDKIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFS 231

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           I IF  GT  YRHKLP GSPF +MA+VIVA+    R+P+  D  + +EL   EY  +  F
Sbjct: 232 IFIFLLGTRLYRHKLPMGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAF 291

Query: 181 RIDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQT 240
            I ST SLRFLN+A+++TGSTH W+LC++T+VEETKQML+M+P+L  TF+PS ML+Q  T
Sbjct: 292 PIHSTSSLRFLNRASLKTGSTHKWRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMT 351

Query: 241 LFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITML 300
           LFIKQG TLDR + ++F IPPASL  F T SMLVS++IYDR FVK M++LT NPRGIT+L
Sbjct: 352 LFIKQGTTLDRRLTNNFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLL 411

Query: 301 QRLGIGMILHILIMTIASLVERHRLHVARKNGL-EKNEEQLPLTIFTLLPQFMLVGVADA 360
           QR+GIGMILHILIM IAS+ ER+RL VA ++GL  +    +PL+IFTLLPQ++L+G+ADA
Sbjct: 412 QRMGIGMILHILIMIIASITERYRLKVAAEHGLTHQTAVPIPLSIFTLLPQYVLMGLADA 471

Query: 361 FSEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGNGWINNNL 420
           F E+AK+EFFYDQAPESMKSLGTSY+ TS+ +G F+SS LLS+VS +TKK G GWI NNL
Sbjct: 472 FIEIAKLEFFYDQAPESMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKKQGRGWIQNNL 531

Query: 421 NASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKV 462
           N S LD YY F AVL+ +NF LFL++ + Y Y+A+V+ S  V
Sbjct: 532 NESRLDNYYMFFAVLNLLNFILFLVVIRFYEYRADVTQSANV 573

BLAST of Bhi01G000499 vs. TAIR 10
Match: AT2G40460.1 (Major facilitator superfamily protein )

HSP 1 Score: 448.0 bits (1151), Expect = 1.0e-125
Identity = 232/456 (50.88%), Postives = 317/456 (69.52%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GM LLT+AV++ SL+P          C + S LQ+  F+ +LY +A+G GGTKPNIST G
Sbjct: 109 GMILLTMAVTVKSLRP----TCENGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFG 168

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFD +  EEK QK+SFFNWWMFS F G LFAT  LVYIQEN+GW LGYGIPTVGL ++
Sbjct: 169 ADQFDSYSIEEKKQKVSFFNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVS 228

Query: 121 ILIFAAGTPFYRHK-LPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGT 180
           +++F  GTPFYRHK + T +    + +V +A   N +   P D  +L+EL    Y   G 
Sbjct: 229 LVVFYIGTPFYRHKVIKTDNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGK 288

Query: 181 FRIDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQ 240
            ++  TP  RFL+KAAI+T S  P   C+VT+VE  K++L +I I + T IPST+ +Q  
Sbjct: 289 HQVHHTPVFRFLDKAAIKTSSRVP---CTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVN 348

Query: 241 TLFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITM 300
           TLF+KQG TLDR +GS+FQIP ASL +FVT+SML+SV +YD+ FV  M++ T NPRGIT+
Sbjct: 349 TLFVKQGTTLDRKIGSNFQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITL 408

Query: 301 LQRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADA 360
           LQRLG+G  + I+ + IAS VE  R+ V ++  +    + +P++IF LLPQ+ L+G+ D 
Sbjct: 409 LQRLGVGFAIQIVAIAIASAVEVKRMRVIKEFHITSPTQVVPMSIFWLLPQYSLLGIGDV 468

Query: 361 FSEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKK-HGNGWINNN 420
           F+ +  +EFFYDQ+PE M+SLGT++  + +G+GNFL+SFL++ +  +T K  G  WI NN
Sbjct: 469 FNAIGLLEFFYDQSPEEMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNN 528

Query: 421 LNASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAE 455
           LN S LDYYYGFL V+S +N  LF+  +  YVYK++
Sbjct: 529 LNDSRLDYYYGFLVVISIVNMGLFVWAASKYVYKSD 557

BLAST of Bhi01G000499 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 388.7 bits (997), Expect = 7.2e-108
Identity = 205/463 (44.28%), Postives = 306/463 (66.09%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GM+LLTL+ S+P LKP  C   NA+ C   S  Q AVFF ALY++ALGTGG KP +S+ G
Sbjct: 109 GMTLLTLSASVPGLKPGNC---NADTCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFG 168

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFD+    EK +K SFFNW+ FSI  G L A T LV+IQ NVGW  G+G+PTV + IA
Sbjct: 169 ADQFDENDENEKIKKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIA 228

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           +  F  G+ FYR + P GSP  R+ +VIVA        +P D   L E   +E   +G+ 
Sbjct: 229 VCFFFFGSRFYRLQRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSR 288

Query: 181 RIDSTPSLRFLNKAA-------IRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPST 240
           ++  T +L+F +KAA       I+ G  +PW+LCSVTQVEE K ++ ++P+     + +T
Sbjct: 289 KLVHTDNLKFFDKAAVESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFAT 348

Query: 241 MLSQAQTLFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNN 300
           + SQ  T+F+ QG T+D+ +G +F+IP ASL+ F T+S+L    +YD+F + + ++ T N
Sbjct: 349 VYSQMSTMFVLQGNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRN 408

Query: 301 PRGITMLQRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFML 360
            RG T LQR+GIG+++ I  M  A ++E  RL   + +    +++Q+ ++IF  +PQ++L
Sbjct: 409 ERGFTQLQRMGIGLVVSIFAMITAGVLEVVRLDYVKTHN-AYDQKQIHMSIFWQIPQYLL 468

Query: 361 VGVADAFSEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGN- 420
           +G A+ F+ + ++EFFYDQAP++M+SL ++ S+T++ +GN+LS+ L++ V  +TKK+G  
Sbjct: 469 IGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKP 528

Query: 421 GWINNNLNASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEV 456
           GWI +NLN  HLDY++  LA LS +NF ++L +SK Y YK  V
Sbjct: 529 GWIPDNLNRGHLDYFFYLLATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of Bhi01G000499 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 379.0 bits (972), Expect = 5.7e-105
Identity = 204/460 (44.35%), Postives = 303/460 (65.87%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GM+LLT++ S+P L P      + E C  T+  Q A+ F ALY++ALGTGG KP +S+ G
Sbjct: 110 GMTLLTISASVPGLTP----TCSGETCHATAG-QTAITFIALYLIALGTGGIKPCVSSFG 169

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFDD   +EK  K SFFNW+ F I  G + A++ LV+IQ NVGW  G G+PTV +AIA
Sbjct: 170 ADQFDDTDEKEKESKSSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIA 229

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           ++ F AG+ FYR + P GSP  RM +VIVA+    +  +P D   L+E Q  E +  G+ 
Sbjct: 230 VVFFFAGSNFYRLQKPGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSR 289

Query: 181 RIDSTPSLRFLNKAAIRTGS-------THPWKLCSVTQVEETKQMLRMIPILICTFIPST 240
           +++ T  L F +KAA+ T S       +  WKLC+VTQVEE K ++R++PI     + ++
Sbjct: 290 KLEHTKILTFFDKAAVETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFAS 349

Query: 241 MLSQAQTLFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNN 300
           + SQ  T+F+ QG TLD+ +G +F+IP ASL+ F T+S+L    +YD+  V   ++ T +
Sbjct: 350 VYSQMGTVFVLQGNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGH 409

Query: 301 PRGITMLQRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFML 360
            RG T LQR+GIG+++ I  M  A ++E  RL+  + + L  NEE +P+TIF  +PQ+ L
Sbjct: 410 ERGFTQLQRIGIGLVISIFSMVSAGILEVARLNYVQTHNL-YNEETIPMTIFWQVPQYFL 469

Query: 361 VGVADAFSEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGN- 420
           VG A+ F+ + ++EFFYDQAP++M+SL ++ S+T++  GN+LS+FL++ V+ +T+  G  
Sbjct: 470 VGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRP 529

Query: 421 GWINNNLNASHLDYYYGFLAVLSTINFFLFLLMSKMYVYK 453
           GWI  NLN  HLDY++  LA LS +NF ++L ++K Y YK
Sbjct: 530 GWIAKNLNNGHLDYFFWLLAGLSFLNFLVYLWIAKWYTYK 563

BLAST of Bhi01G000499 vs. ExPASy Swiss-Prot
Match: Q9FNL7 (Protein NRT1/ PTR FAMILY 5.2 OS=Arabidopsis thaliana OX=3702 GN=NPF5.2 PE=2 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 2.5e-177
Identity = 308/468 (65.81%), Postives = 377/468 (80.56%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GM +LTL+V++P +KP  C   N E+C++ S LQLAVFF ALY LA+GTGGTKPNISTIG
Sbjct: 112 GMMVLTLSVTIPGIKPPECSTTNVENCEKASVLQLAVFFGALYTLAIGTGGTKPNISTIG 171

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFD F P+EK QKLSFFNWWMFSIFFGTLFA T LVY+Q+NVGW+LGYG+PT+GLAI+
Sbjct: 172 ADQFDVFDPKEKTQKLSFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAIS 231

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           I IF  GTPFYRHKLPTGSPF +MA+VIVA+      P+ +D    HEL   EY ++G F
Sbjct: 232 ITIFLLGTPFYRHKLPTGSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAF 291

Query: 181 RIDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQT 240
            I  TPSLRFL++A+++TG+ H W LC+ T+VEETKQMLRM+P+L  TF+PS ML+Q  T
Sbjct: 292 PIHPTPSLRFLDRASLKTGTNHKWNLCTTTEVEETKQMLRMLPVLFITFVPSMMLAQINT 351

Query: 241 LFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITML 300
           LF+KQG TLDR V   F IPPASL+ FVT+SML+S+++YDR FVKI ++ T NPRGIT+L
Sbjct: 352 LFVKQGTTLDRKVTGSFSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLL 411

Query: 301 QRLGIGMILHILIMTIASLVERHRLHVARKNGL-EKNEEQLPLTIFTLLPQFMLVGVADA 360
           QR+GIG+I HILIM +AS+ ER+RL VA  +GL  +   +LPLTIF LLPQF+L+G+AD+
Sbjct: 412 QRMGIGLIFHILIMIVASVTERYRLKVAADHGLIHQTGVKLPLTIFALLPQFVLMGMADS 471

Query: 361 FSEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGNGWINNNL 420
           F EVAK+EFFYDQAPESMKSLGTSYS TSL IGNF+SSFLLSTVS +TKK G GWI NNL
Sbjct: 472 FLEVAKLEFFYDQAPESMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKRGRGWILNNL 531

Query: 421 NASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTDKLK 468
           N S LDYYY F AVL+ +NF LFL++ K YVY+AEV+DS+ V   ++K
Sbjct: 532 NESRLDYYYLFFAVLNLVNFVLFLVVVKFYVYRAEVTDSVDVKEVEMK 579

BLAST of Bhi01G000499 vs. ExPASy Swiss-Prot
Match: Q9FNL8 (Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 1.9e-169
Identity = 294/462 (63.64%), Postives = 369/462 (79.87%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GM+LLTL+VS+P LKP  C  AN E+C++ S +QLAVFF ALY LA+GTGGTKPNISTIG
Sbjct: 112 GMALLTLSVSLPGLKPPKCSTANVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIG 171

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFD+F P++K  K SFFNWWMFSIFFGT FATT LVY+Q+NVGW++GYG+ T+GLA +
Sbjct: 172 ADQFDEFDPKDKIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFS 231

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           I IF  GT  YRHKLP GSPF +MA+VIVA+    R+P+  D  + +EL   EY  +  F
Sbjct: 232 IFIFLLGTRLYRHKLPMGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAF 291

Query: 181 RIDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQT 240
            I ST SLRFLN+A+++TGSTH W+LC++T+VEETKQML+M+P+L  TF+PS ML+Q  T
Sbjct: 292 PIHSTSSLRFLNRASLKTGSTHKWRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMT 351

Query: 241 LFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITML 300
           LFIKQG TLDR + ++F IPPASL  F T SMLVS++IYDR FVK M++LT NPRGIT+L
Sbjct: 352 LFIKQGTTLDRRLTNNFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLL 411

Query: 301 QRLGIGMILHILIMTIASLVERHRLHVARKNGL-EKNEEQLPLTIFTLLPQFMLVGVADA 360
           QR+GIGMILHILIM IAS+ ER+RL VA ++GL  +    +PL+IFTLLPQ++L+G+ADA
Sbjct: 412 QRMGIGMILHILIMIIASITERYRLKVAAEHGLTHQTAVPIPLSIFTLLPQYVLMGLADA 471

Query: 361 FSEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGNGWINNNL 420
           F E+AK+EFFYDQAPESMKSLGTSY+ TS+ +G F+SS LLS+VS +TKK G GWI NNL
Sbjct: 472 FIEIAKLEFFYDQAPESMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKKQGRGWIQNNL 531

Query: 421 NASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKV 462
           N S LD YY F AVL+ +NF LFL++ + Y Y+A+V+ S  V
Sbjct: 532 NESRLDNYYMFFAVLNLLNFILFLVVIRFYEYRADVTQSANV 573

BLAST of Bhi01G000499 vs. ExPASy Swiss-Prot
Match: Q8VZR7 (Protein NRT1/ PTR FAMILY 5.1 OS=Arabidopsis thaliana OX=3702 GN=NPF5.1 PE=2 SV=2)

HSP 1 Score: 448.0 bits (1151), Expect = 1.4e-124
Identity = 232/456 (50.88%), Postives = 317/456 (69.52%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GM LLT+AV++ SL+P          C + S LQ+  F+ +LY +A+G GGTKPNIST G
Sbjct: 109 GMILLTMAVTVKSLRP----TCENGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFG 168

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFD +  EEK QK+SFFNWWMFS F G LFAT  LVYIQEN+GW LGYGIPTVGL ++
Sbjct: 169 ADQFDSYSIEEKKQKVSFFNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVS 228

Query: 121 ILIFAAGTPFYRHK-LPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGT 180
           +++F  GTPFYRHK + T +    + +V +A   N +   P D  +L+EL    Y   G 
Sbjct: 229 LVVFYIGTPFYRHKVIKTDNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGK 288

Query: 181 FRIDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQ 240
            ++  TP  RFL+KAAI+T S  P   C+VT+VE  K++L +I I + T IPST+ +Q  
Sbjct: 289 HQVHHTPVFRFLDKAAIKTSSRVP---CTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVN 348

Query: 241 TLFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITM 300
           TLF+KQG TLDR +GS+FQIP ASL +FVT+SML+SV +YD+ FV  M++ T NPRGIT+
Sbjct: 349 TLFVKQGTTLDRKIGSNFQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITL 408

Query: 301 LQRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADA 360
           LQRLG+G  + I+ + IAS VE  R+ V ++  +    + +P++IF LLPQ+ L+G+ D 
Sbjct: 409 LQRLGVGFAIQIVAIAIASAVEVKRMRVIKEFHITSPTQVVPMSIFWLLPQYSLLGIGDV 468

Query: 361 FSEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKK-HGNGWINNN 420
           F+ +  +EFFYDQ+PE M+SLGT++  + +G+GNFL+SFL++ +  +T K  G  WI NN
Sbjct: 469 FNAIGLLEFFYDQSPEEMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNN 528

Query: 421 LNASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAE 455
           LN S LDYYYGFL V+S +N  LF+  +  YVYK++
Sbjct: 529 LNDSRLDYYYGFLVVISIVNMGLFVWAASKYVYKSD 557

BLAST of Bhi01G000499 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.0e-106
Identity = 205/463 (44.28%), Postives = 306/463 (66.09%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GM+LLTL+ S+P LKP  C   NA+ C   S  Q AVFF ALY++ALGTGG KP +S+ G
Sbjct: 109 GMTLLTLSASVPGLKPGNC---NADTCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFG 168

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFD+    EK +K SFFNW+ FSI  G L A T LV+IQ NVGW  G+G+PTV + IA
Sbjct: 169 ADQFDENDENEKIKKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIA 228

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           +  F  G+ FYR + P GSP  R+ +VIVA        +P D   L E   +E   +G+ 
Sbjct: 229 VCFFFFGSRFYRLQRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSR 288

Query: 181 RIDSTPSLRFLNKAA-------IRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPST 240
           ++  T +L+F +KAA       I+ G  +PW+LCSVTQVEE K ++ ++P+     + +T
Sbjct: 289 KLVHTDNLKFFDKAAVESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFAT 348

Query: 241 MLSQAQTLFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNN 300
           + SQ  T+F+ QG T+D+ +G +F+IP ASL+ F T+S+L    +YD+F + + ++ T N
Sbjct: 349 VYSQMSTMFVLQGNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRN 408

Query: 301 PRGITMLQRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFML 360
            RG T LQR+GIG+++ I  M  A ++E  RL   + +    +++Q+ ++IF  +PQ++L
Sbjct: 409 ERGFTQLQRMGIGLVVSIFAMITAGVLEVVRLDYVKTHN-AYDQKQIHMSIFWQIPQYLL 468

Query: 361 VGVADAFSEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGN- 420
           +G A+ F+ + ++EFFYDQAP++M+SL ++ S+T++ +GN+LS+ L++ V  +TKK+G  
Sbjct: 469 IGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKP 528

Query: 421 GWINNNLNASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEV 456
           GWI +NLN  HLDY++  LA LS +NF ++L +SK Y YK  V
Sbjct: 529 GWIPDNLNRGHLDYFFYLLATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of Bhi01G000499 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 8.1e-104
Identity = 204/460 (44.35%), Postives = 303/460 (65.87%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GM+LLT++ S+P L P      + E C  T+  Q A+ F ALY++ALGTGG KP +S+ G
Sbjct: 110 GMTLLTISASVPGLTP----TCSGETCHATAG-QTAITFIALYLIALGTGGIKPCVSSFG 169

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFDD   +EK  K SFFNW+ F I  G + A++ LV+IQ NVGW  G G+PTV +AIA
Sbjct: 170 ADQFDDTDEKEKESKSSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIA 229

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           ++ F AG+ FYR + P GSP  RM +VIVA+    +  +P D   L+E Q  E +  G+ 
Sbjct: 230 VVFFFAGSNFYRLQKPGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSR 289

Query: 181 RIDSTPSLRFLNKAAIRTGS-------THPWKLCSVTQVEETKQMLRMIPILICTFIPST 240
           +++ T  L F +KAA+ T S       +  WKLC+VTQVEE K ++R++PI     + ++
Sbjct: 290 KLEHTKILTFFDKAAVETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFAS 349

Query: 241 MLSQAQTLFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNN 300
           + SQ  T+F+ QG TLD+ +G +F+IP ASL+ F T+S+L    +YD+  V   ++ T +
Sbjct: 350 VYSQMGTVFVLQGNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGH 409

Query: 301 PRGITMLQRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFML 360
            RG T LQR+GIG+++ I  M  A ++E  RL+  + + L  NEE +P+TIF  +PQ+ L
Sbjct: 410 ERGFTQLQRIGIGLVISIFSMVSAGILEVARLNYVQTHNL-YNEETIPMTIFWQVPQYFL 469

Query: 361 VGVADAFSEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGN- 420
           VG A+ F+ + ++EFFYDQAP++M+SL ++ S+T++  GN+LS+FL++ V+ +T+  G  
Sbjct: 470 VGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRP 529

Query: 421 GWINNNLNASHLDYYYGFLAVLSTINFFLFLLMSKMYVYK 453
           GWI  NLN  HLDY++  LA LS +NF ++L ++K Y YK
Sbjct: 530 GWIAKNLNNGHLDYFFWLLAGLSFLNFLVYLWIAKWYTYK 563

BLAST of Bhi01G000499 vs. NCBI nr
Match: XP_038902958.1 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 979.9 bits (2532), Expect = 7.9e-282
Identity = 495/495 (100.00%), Postives = 495/495 (100.00%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG
Sbjct: 123 GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 182

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA
Sbjct: 183 ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 242

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF
Sbjct: 243 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 302

Query: 181 RIDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQT 240
           RIDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQT
Sbjct: 303 RIDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQT 362

Query: 241 LFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITML 300
           LFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITML
Sbjct: 363 LFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITML 422

Query: 301 QRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAF 360
           QRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAF
Sbjct: 423 QRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAF 482

Query: 361 SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGNGWINNNLN 420
           SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGNGWINNNLN
Sbjct: 483 SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGNGWINNNLN 542

Query: 421 ASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTDKLKASNLNEKSAMTRG 480
           ASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTDKLKASNLNEKSAMTRG
Sbjct: 543 ASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTDKLKASNLNEKSAMTRG 602

Query: 481 RADEGRGPLPPFNLF 496
           RADEGRGPLPPFNLF
Sbjct: 603 RADEGRGPLPPFNLF 617

BLAST of Bhi01G000499 vs. NCBI nr
Match: XP_038902959.1 (protein NRT1/ PTR FAMILY 5.2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 977.2 bits (2525), Expect = 5.1e-281
Identity = 494/494 (100.00%), Postives = 494/494 (100.00%), Query Frame = 0

Query: 2   MSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIGA 61
           MSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIGA
Sbjct: 1   MSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIGA 60

Query: 62  DQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIAI 121
           DQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIAI
Sbjct: 61  DQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIAI 120

Query: 122 LIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTFR 181
           LIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTFR
Sbjct: 121 LIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTFR 180

Query: 182 IDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQTL 241
           IDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQTL
Sbjct: 181 IDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQTL 240

Query: 242 FIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITMLQ 301
           FIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITMLQ
Sbjct: 241 FIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITMLQ 300

Query: 302 RLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAFS 361
           RLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAFS
Sbjct: 301 RLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAFS 360

Query: 362 EVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGNGWINNNLNA 421
           EVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGNGWINNNLNA
Sbjct: 361 EVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGNGWINNNLNA 420

Query: 422 SHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTDKLKASNLNEKSAMTRGR 481
           SHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTDKLKASNLNEKSAMTRGR
Sbjct: 421 SHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTDKLKASNLNEKSAMTRGR 480

Query: 482 ADEGRGPLPPFNLF 496
           ADEGRGPLPPFNLF
Sbjct: 481 ADEGRGPLPPFNLF 494

BLAST of Bhi01G000499 vs. NCBI nr
Match: XP_004134404.1 (protein NRT1/ PTR FAMILY 5.2 [Cucumis sativus] >KGN56863.2 hypothetical protein Csa_010990 [Cucumis sativus])

HSP 1 Score: 895.2 bits (2312), Expect = 2.6e-256
Identity = 455/498 (91.37%), Postives = 471/498 (94.58%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GMSLLTLAVSMPSLKP PCLD N E+CKQ SRL LA+FFTALYILALGTGGTKPNISTIG
Sbjct: 125 GMSLLTLAVSMPSLKPPPCLDTNKENCKQASRLHLAIFFTALYILALGTGGTKPNISTIG 184

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFDDFHP EKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA
Sbjct: 185 ADQFDDFHPGEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 244

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQ +P D RQL+ELQLEEY+KQGTF
Sbjct: 245 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQDIPDDSRQLYELQLEEYSKQGTF 304

Query: 181 RIDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQT 240
           RIDSTPSLRFLNKAAIRTGSTH WKLC+VTQVEETKQMLRMIPILICTFIPSTML QAQT
Sbjct: 305 RIDSTPSLRFLNKAAIRTGSTHSWKLCTVTQVEETKQMLRMIPILICTFIPSTMLPQAQT 364

Query: 241 LFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITML 300
           LFIKQGRTL+RSVGS FQIPPASLTAFVTISML+SVIIYDRFFVKIMQ+LTNNPRGIT+L
Sbjct: 365 LFIKQGRTLNRSVGSQFQIPPASLTAFVTISMLISVIIYDRFFVKIMQKLTNNPRGITLL 424

Query: 301 QRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAF 360
           QR+GIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQL LTIFTLLPQFMLVGVADAF
Sbjct: 425 QRMGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLSLTIFTLLPQFMLVGVADAF 484

Query: 361 SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKH----GNGWIN 420
           SEVAKIEFFYDQAPE+MKSLGTSYSMTSLGIGNFLSSFLLSTVSH+TKKH    GNGWI 
Sbjct: 485 SEVAKIEFFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITKKHGNGNGNGWIE 544

Query: 421 NNLNASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTDKLKASNLNEKSA 480
           NNLN SHLDYYY FLAVL  INFF FL+MSKMY YKAEVSDSIKVL+DKLKASN+NEK A
Sbjct: 545 NNLNVSHLDYYYAFLAVLCAINFFFFLVMSKMYEYKAEVSDSIKVLSDKLKASNINEKPA 604

Query: 481 MTRGRADEGRGPLPPFNL 495
           M RGRADEGRG LPPFNL
Sbjct: 605 MMRGRADEGRGHLPPFNL 622

BLAST of Bhi01G000499 vs. NCBI nr
Match: XP_016898953.1 (PREDICTED: LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo])

HSP 1 Score: 892.1 bits (2304), Expect = 2.2e-255
Identity = 455/492 (92.48%), Postives = 470/492 (95.53%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GMSLLTLAVSMPSLKP PCL+ N E+CKQTSRLQLA+FFTALY LALGTGGTKPNISTIG
Sbjct: 123 GMSLLTLAVSMPSLKPPPCLETNKENCKQTSRLQLAIFFTALYTLALGTGGTKPNISTIG 182

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFDDFHP EKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA
Sbjct: 183 ADQFDDFHPGEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 242

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATA NWRQ +P D RQL+ELQLEEY+KQGTF
Sbjct: 243 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATALNWRQDIPNDSRQLYELQLEEYSKQGTF 302

Query: 181 RIDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQT 240
           RIDST SLRFLNKAAIRTGSTH WKLC+VTQVEETKQMLRMIPILICTFIPSTML QAQT
Sbjct: 303 RIDSTSSLRFLNKAAIRTGSTHSWKLCTVTQVEETKQMLRMIPILICTFIPSTMLPQAQT 362

Query: 241 LFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITML 300
           LFIKQGRTL+RSVGS FQIPPASLTAFVTISML+SVIIYDRFFVKIMQ+LTNNPRGIT+L
Sbjct: 363 LFIKQGRTLNRSVGSQFQIPPASLTAFVTISMLISVIIYDRFFVKIMQKLTNNPRGITLL 422

Query: 301 QRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAF 360
           QR+GIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAF
Sbjct: 423 QRMGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAF 482

Query: 361 SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHG-NGWINNNL 420
           SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSH+TKKHG NGWI NNL
Sbjct: 483 SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITKKHGNNGWIENNL 542

Query: 421 NASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTDKLKASNLNEKSAMTR 480
           N SHLDYYY FLAVLS INFF FL+MSKMY YKAEVSDSIKVLTDKLKASN+NEKSAM R
Sbjct: 543 NVSHLDYYYAFLAVLSAINFFFFLVMSKMYEYKAEVSDSIKVLTDKLKASNINEKSAMMR 602

Query: 481 GRADEGRGPLPP 492
           GRADEGRG LPP
Sbjct: 603 GRADEGRGHLPP 614

BLAST of Bhi01G000499 vs. NCBI nr
Match: KAA0049225.1 (protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa] >TYK17333.1 protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa])

HSP 1 Score: 890.6 bits (2300), Expect = 6.3e-255
Identity = 454/492 (92.28%), Postives = 469/492 (95.33%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GMSLLTLAVSMPSLKP PCL+ N E+CKQTSRLQLA+FFTALY LALGTGGTKPNISTIG
Sbjct: 123 GMSLLTLAVSMPSLKPPPCLETNKENCKQTSRLQLAIFFTALYTLALGTGGTKPNISTIG 182

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFDDFHP EKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA
Sbjct: 183 ADQFDDFHPGEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 242

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATA NWRQ +P D RQL+ELQLEEY+KQGTF
Sbjct: 243 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATALNWRQDIPNDSRQLYELQLEEYSKQGTF 302

Query: 181 RIDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQT 240
           RIDST SLRFLNKAAIRTGSTH WKLC+VTQVEETKQMLRMIPILICTFIPSTML QAQT
Sbjct: 303 RIDSTSSLRFLNKAAIRTGSTHSWKLCTVTQVEETKQMLRMIPILICTFIPSTMLPQAQT 362

Query: 241 LFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITML 300
           LFIKQGRTL+RSVGS FQIPPASLTAFVTISML+SVIIYDRFFVKIMQ+LTNNPRGIT+L
Sbjct: 363 LFIKQGRTLNRSVGSQFQIPPASLTAFVTISMLISVIIYDRFFVKIMQKLTNNPRGITLL 422

Query: 301 QRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAF 360
           QR+GIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTL PQFMLVGVADAF
Sbjct: 423 QRMGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLFPQFMLVGVADAF 482

Query: 361 SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHG-NGWINNNL 420
           SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSH+TKKHG NGWI NNL
Sbjct: 483 SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITKKHGNNGWIENNL 542

Query: 421 NASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTDKLKASNLNEKSAMTR 480
           N SHLDYYY FLAVLS INFF FL+MSKMY YKAEVSDSIKVLTDKLKASN+NEKSAM R
Sbjct: 543 NVSHLDYYYAFLAVLSAINFFFFLVMSKMYEYKAEVSDSIKVLTDKLKASNINEKSAMMR 602

Query: 481 GRADEGRGPLPP 492
           GRADEGRG LPP
Sbjct: 603 GRADEGRGHLPP 614

BLAST of Bhi01G000499 vs. ExPASy TrEMBL
Match: A0A0A0L4B7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G134780 PE=3 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.2e-256
Identity = 455/498 (91.37%), Postives = 471/498 (94.58%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GMSLLTLAVSMPSLKP PCLD N E+CKQ SRL LA+FFTALYILALGTGGTKPNISTIG
Sbjct: 150 GMSLLTLAVSMPSLKPPPCLDTNKENCKQASRLHLAIFFTALYILALGTGGTKPNISTIG 209

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFDDFHP EKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA
Sbjct: 210 ADQFDDFHPGEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 269

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQ +P D RQL+ELQLEEY+KQGTF
Sbjct: 270 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQDIPDDSRQLYELQLEEYSKQGTF 329

Query: 181 RIDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQT 240
           RIDSTPSLRFLNKAAIRTGSTH WKLC+VTQVEETKQMLRMIPILICTFIPSTML QAQT
Sbjct: 330 RIDSTPSLRFLNKAAIRTGSTHSWKLCTVTQVEETKQMLRMIPILICTFIPSTMLPQAQT 389

Query: 241 LFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITML 300
           LFIKQGRTL+RSVGS FQIPPASLTAFVTISML+SVIIYDRFFVKIMQ+LTNNPRGIT+L
Sbjct: 390 LFIKQGRTLNRSVGSQFQIPPASLTAFVTISMLISVIIYDRFFVKIMQKLTNNPRGITLL 449

Query: 301 QRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAF 360
           QR+GIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQL LTIFTLLPQFMLVGVADAF
Sbjct: 450 QRMGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLSLTIFTLLPQFMLVGVADAF 509

Query: 361 SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKH----GNGWIN 420
           SEVAKIEFFYDQAPE+MKSLGTSYSMTSLGIGNFLSSFLLSTVSH+TKKH    GNGWI 
Sbjct: 510 SEVAKIEFFYDQAPENMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITKKHGNGNGNGWIE 569

Query: 421 NNLNASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTDKLKASNLNEKSA 480
           NNLN SHLDYYY FLAVL  INFF FL+MSKMY YKAEVSDSIKVL+DKLKASN+NEK A
Sbjct: 570 NNLNVSHLDYYYAFLAVLCAINFFFFLVMSKMYEYKAEVSDSIKVLSDKLKASNINEKPA 629

Query: 481 MTRGRADEGRGPLPPFNL 495
           M RGRADEGRG LPPFNL
Sbjct: 630 MMRGRADEGRGHLPPFNL 647

BLAST of Bhi01G000499 vs. ExPASy TrEMBL
Match: A0A1S4DSL3 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo OX=3656 GN=LOC107990387 PE=3 SV=1)

HSP 1 Score: 892.1 bits (2304), Expect = 1.0e-255
Identity = 455/492 (92.48%), Postives = 470/492 (95.53%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GMSLLTLAVSMPSLKP PCL+ N E+CKQTSRLQLA+FFTALY LALGTGGTKPNISTIG
Sbjct: 123 GMSLLTLAVSMPSLKPPPCLETNKENCKQTSRLQLAIFFTALYTLALGTGGTKPNISTIG 182

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFDDFHP EKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA
Sbjct: 183 ADQFDDFHPGEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 242

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATA NWRQ +P D RQL+ELQLEEY+KQGTF
Sbjct: 243 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATALNWRQDIPNDSRQLYELQLEEYSKQGTF 302

Query: 181 RIDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQT 240
           RIDST SLRFLNKAAIRTGSTH WKLC+VTQVEETKQMLRMIPILICTFIPSTML QAQT
Sbjct: 303 RIDSTSSLRFLNKAAIRTGSTHSWKLCTVTQVEETKQMLRMIPILICTFIPSTMLPQAQT 362

Query: 241 LFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITML 300
           LFIKQGRTL+RSVGS FQIPPASLTAFVTISML+SVIIYDRFFVKIMQ+LTNNPRGIT+L
Sbjct: 363 LFIKQGRTLNRSVGSQFQIPPASLTAFVTISMLISVIIYDRFFVKIMQKLTNNPRGITLL 422

Query: 301 QRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAF 360
           QR+GIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAF
Sbjct: 423 QRMGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAF 482

Query: 361 SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHG-NGWINNNL 420
           SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSH+TKKHG NGWI NNL
Sbjct: 483 SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITKKHGNNGWIENNL 542

Query: 421 NASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTDKLKASNLNEKSAMTR 480
           N SHLDYYY FLAVLS INFF FL+MSKMY YKAEVSDSIKVLTDKLKASN+NEKSAM R
Sbjct: 543 NVSHLDYYYAFLAVLSAINFFFFLVMSKMYEYKAEVSDSIKVLTDKLKASNINEKSAMMR 602

Query: 481 GRADEGRGPLPP 492
           GRADEGRG LPP
Sbjct: 603 GRADEGRGHLPP 614

BLAST of Bhi01G000499 vs. ExPASy TrEMBL
Match: A0A5A7U6D9 (Protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001880 PE=3 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 3.0e-255
Identity = 454/492 (92.28%), Postives = 469/492 (95.33%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GMSLLTLAVSMPSLKP PCL+ N E+CKQTSRLQLA+FFTALY LALGTGGTKPNISTIG
Sbjct: 123 GMSLLTLAVSMPSLKPPPCLETNKENCKQTSRLQLAIFFTALYTLALGTGGTKPNISTIG 182

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFDDFHP EKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA
Sbjct: 183 ADQFDDFHPGEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 242

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATA NWRQ +P D RQL+ELQLEEY+KQGTF
Sbjct: 243 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATALNWRQDIPNDSRQLYELQLEEYSKQGTF 302

Query: 181 RIDSTPSLRFLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQT 240
           RIDST SLRFLNKAAIRTGSTH WKLC+VTQVEETKQMLRMIPILICTFIPSTML QAQT
Sbjct: 303 RIDSTSSLRFLNKAAIRTGSTHSWKLCTVTQVEETKQMLRMIPILICTFIPSTMLPQAQT 362

Query: 241 LFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITML 300
           LFIKQGRTL+RSVGS FQIPPASLTAFVTISML+SVIIYDRFFVKIMQ+LTNNPRGIT+L
Sbjct: 363 LFIKQGRTLNRSVGSQFQIPPASLTAFVTISMLISVIIYDRFFVKIMQKLTNNPRGITLL 422

Query: 301 QRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAF 360
           QR+GIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTL PQFMLVGVADAF
Sbjct: 423 QRMGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLFPQFMLVGVADAF 482

Query: 361 SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHG-NGWINNNL 420
           SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSH+TKKHG NGWI NNL
Sbjct: 483 SEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITKKHGNNGWIENNL 542

Query: 421 NASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTDKLKASNLNEKSAMTR 480
           N SHLDYYY FLAVLS INFF FL+MSKMY YKAEVSDSIKVLTDKLKASN+NEKSAM R
Sbjct: 543 NVSHLDYYYAFLAVLSAINFFFFLVMSKMYEYKAEVSDSIKVLTDKLKASNINEKSAMMR 602

Query: 481 GRADEGRGPLPP 492
           GRADEGRG LPP
Sbjct: 603 GRADEGRGHLPP 614

BLAST of Bhi01G000499 vs. ExPASy TrEMBL
Match: A0A6J1ECE4 (protein NRT1/ PTR FAMILY 5.2-like OS=Cucurbita moschata OX=3662 GN=LOC111431933 PE=3 SV=1)

HSP 1 Score: 857.8 bits (2215), Expect = 2.2e-245
Identity = 441/501 (88.02%), Postives = 463/501 (92.42%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GMSLLTLAVSMPSLKP PCL+ N EHCK  SRL LAVFFTALY LALGTGGTKPNISTIG
Sbjct: 117 GMSLLTLAVSMPSLKPPPCLEPNIEHCKPASRLHLAVFFTALYTLALGTGGTKPNISTIG 176

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTL ATTTLVYIQ+NVGWSLGYGIPTVGLAIA
Sbjct: 177 ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLLATTTLVYIQDNVGWSLGYGIPTVGLAIA 236

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           ILIFAAGTP YRHKLPTGSPF RMAKVIVATAWNWRQPLP DPRQLHELQLEEYTK+G F
Sbjct: 237 ILIFAAGTPMYRHKLPTGSPFSRMAKVIVATAWNWRQPLPNDPRQLHELQLEEYTKKGAF 296

Query: 181 RIDSTPSLRFLNKAAIRTG-STHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQ 240
           RIDSTPSLRFLNKAAIRTG STHPWKLC+VTQVEETKQMLRMIPILICTFIPSTML QA 
Sbjct: 297 RIDSTPSLRFLNKAAIRTGSSTHPWKLCTVTQVEETKQMLRMIPILICTFIPSTMLPQAH 356

Query: 241 TLFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITM 300
           TLFIKQGRTL+RS GS+FQIPPASLTAFVTISMLVSVIIYDR FVKIMQRLTNNPRGIT+
Sbjct: 357 TLFIKQGRTLNRSFGSNFQIPPASLTAFVTISMLVSVIIYDRLFVKIMQRLTNNPRGITL 416

Query: 301 LQRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADA 360
           LQR+GIGMILHILIMTIASLVERHRL VAR+NG   + ++LPLTIFTLLPQFMLVGVADA
Sbjct: 417 LQRMGIGMILHILIMTIASLVERHRLDVARQNG---SRQELPLTIFTLLPQFMLVGVADA 476

Query: 361 FSEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGNGWINNNL 420
           F+EVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSH+T+KHGNGWI N+L
Sbjct: 477 FTEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGNGWIMNDL 536

Query: 421 NASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTD-----KLKASNLNEK 480
           NASHLDYYY FLAVLS INFFLFLL+SK YVYKAEVSDS+KVLT+     KLK SN +EK
Sbjct: 537 NASHLDYYYAFLAVLSAINFFLFLLISKFYVYKAEVSDSLKVLTEKLKENKLKPSNPDEK 596

Query: 481 SAMTRGRADEGRGPLPPFNLF 496
            A TR R+DEGR  LPPF+LF
Sbjct: 597 WATTRARSDEGRAHLPPFSLF 614

BLAST of Bhi01G000499 vs. ExPASy TrEMBL
Match: A0A6J1IT17 (protein NRT1/ PTR FAMILY 5.2-like OS=Cucurbita maxima OX=3661 GN=LOC111479111 PE=3 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.1e-244
Identity = 440/501 (87.82%), Postives = 461/501 (92.02%), Query Frame = 0

Query: 1   GMSLLTLAVSMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIG 60
           GMSLLTLAVSMPSLKP PC + N EHCK  SRL LAVFFTALY LALGTGGTKPNISTIG
Sbjct: 128 GMSLLTLAVSMPSLKPPPCSEPNIEHCKPASRLHLAVFFTALYTLALGTGGTKPNISTIG 187

Query: 61  ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIA 120
           ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQ+NVGWSLGYGIPTVGLAIA
Sbjct: 188 ADQFDDFHPEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQDNVGWSLGYGIPTVGLAIA 247

Query: 121 ILIFAAGTPFYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTF 180
           ILIFAAGTP YRHKLPTGSPFIRMAKVI ATAWNWRQPLP DPRQLHELQLEEYTK+G F
Sbjct: 248 ILIFAAGTPLYRHKLPTGSPFIRMAKVIFATAWNWRQPLPNDPRQLHELQLEEYTKKGAF 307

Query: 181 RIDSTPSLRFLNKAAIRTG-STHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQ 240
           RIDSTPSLRFLNKAAIR G STHPWKLC+VTQVEETKQMLRMIPILICTFIPSTML QA 
Sbjct: 308 RIDSTPSLRFLNKAAIRIGSSTHPWKLCTVTQVEETKQMLRMIPILICTFIPSTMLPQAH 367

Query: 241 TLFIKQGRTLDRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITM 300
           TLFIKQGRTL+RS GS+FQIPPASLTAFVTISMLVSVIIYDR FVKIMQRLTNNPRGIT+
Sbjct: 368 TLFIKQGRTLNRSFGSNFQIPPASLTAFVTISMLVSVIIYDRLFVKIMQRLTNNPRGITL 427

Query: 301 LQRLGIGMILHILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADA 360
           LQR+GIGMILHILIMTIASLVERHRL VAR+NG   + ++LPLTIFTLLPQFMLVGVADA
Sbjct: 428 LQRMGIGMILHILIMTIASLVERHRLDVARQNG---SRQELPLTIFTLLPQFMLVGVADA 487

Query: 361 FSEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKHGNGWINNNL 420
           F+EVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSH+T+KHGNGWI N+L
Sbjct: 488 FTEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGNGWIMNDL 547

Query: 421 NASHLDYYYGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTD-----KLKASNLNEK 480
           NASHLDYYY FLAVLS INFFLFLL+SK YVYKAEVSDS+KVLT+     KLK SN NEK
Sbjct: 548 NASHLDYYYAFLAVLSAINFFLFLLISKFYVYKAEVSDSLKVLTEKLKENKLKPSNPNEK 607

Query: 481 SAMTRGRADEGRGPLPPFNLF 496
            A TR  +DEGR  LPPF+LF
Sbjct: 608 WATTRATSDEGRAHLPPFSLF 625

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G46050.11.7e-17865.81peptide transporter 3 [more]
AT5G46040.11.3e-17063.64Major facilitator superfamily protein [more]
AT2G40460.11.0e-12550.88Major facilitator superfamily protein [more]
AT3G54140.17.2e-10844.28peptide transporter 1 [more]
AT5G01180.15.7e-10544.35peptide transporter 5 [more]
Match NameE-valueIdentityDescription
Q9FNL72.5e-17765.81Protein NRT1/ PTR FAMILY 5.2 OS=Arabidopsis thaliana OX=3702 GN=NPF5.2 PE=2 SV=1[more]
Q9FNL81.9e-16963.64Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1[more]
Q8VZR71.4e-12450.88Protein NRT1/ PTR FAMILY 5.1 OS=Arabidopsis thaliana OX=3702 GN=NPF5.1 PE=2 SV=2[more]
Q9M3901.0e-10644.28Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB88.1e-10444.35Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038902958.17.9e-282100.00protein NRT1/ PTR FAMILY 5.2-like isoform X1 [Benincasa hispida][more]
XP_038902959.15.1e-281100.00protein NRT1/ PTR FAMILY 5.2-like isoform X2 [Benincasa hispida][more]
XP_004134404.12.6e-25691.37protein NRT1/ PTR FAMILY 5.2 [Cucumis sativus] >KGN56863.2 hypothetical protein ... [more]
XP_016898953.12.2e-25592.48PREDICTED: LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo][more]
KAA0049225.16.3e-25592.28protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa] >TYK17333.1 protein... [more]
Match NameE-valueIdentityDescription
A0A0A0L4B71.2e-25691.37Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G134780 PE=3 SV=1[more]
A0A1S4DSL31.0e-25592.48LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo OX=3656 G... [more]
A0A5A7U6D93.0e-25592.28Protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A6J1ECE42.2e-24588.02protein NRT1/ PTR FAMILY 5.2-like OS=Cucurbita moschata OX=3662 GN=LOC111431933 ... [more]
A0A6J1IT171.1e-24487.82protein NRT1/ PTR FAMILY 5.2-like OS=Cucurbita maxima OX=3661 GN=LOC111479111 PE... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 1..414
e-value: 4.2E-102
score: 341.9
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 1..463
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..457
e-value: 4.6E-148
score: 495.7
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 35..447
NoneNo IPR availablePANTHERPTHR11654:SF453PROTEIN NRT1/ PTR FAMILY 5.2-RELATEDcoord: 1..463

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M000499Bhi01M000499mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035442 dipeptide transmembrane transport
biological_process GO:0042939 tripeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0071916 dipeptide transmembrane transporter activity
molecular_function GO:0042937 tripeptide transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity