Spg033738 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg033738
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCysteine protease
Locationscaffold13: 38425923 .. 38431450 (-)
RNA-Seq ExpressionSpg033738
SyntenySpg033738
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCATTCCCCTCTGTTTCAGTACAGTCTCTACCAATGGAGCGCCTCACTGTAATCGCCTTCTTCTTTATCATTCTACTATCGACGACGGTAGCCTACGGCGTATCTTCCGATCAAATCAACGGCGCCAACGAAGACGATCTTCTGATTCGTCAAGTCGTGTCCGGTGCCGATGATCGTCTCTTAACCGCCGAGCAACACTTCCAGAACTTCAAACTCAAGTTCGGAAAGACCTACGCCACTGATGAGGAGCACGATTACAGGTTCCGTGTCTTCAAGGCTAACCTTCGCAGAGCCAAACGCCACCAGAAGCTCGATCCCGGCGCCGTCCATGGCGTCACTCGGTTTTCGGATCTCACCGAGTCCGAGTTCCGTAAAAATTTTGTTGGAGTGAATCGGCTCCGTCTTCCTGCCGATGCTCACGAGGCTCCGATTCTTCCTACCGATGACCTTGCCTCCGACTTCGATTGGCGCAACCTCGGTGCCGTCACGCCAGTCAAAGATCAGGTACGAGTTTCGGAACGAACTTGTTCCTCACTTTTGTCGAGTATTTGACCTCCGATTTTCCTGTTTTCTTGAGAGAAGCGTTTGCTCCAATTTTTTTAGGGTTCTTGTGGCTCGTGCTGGTCTTTCAGTGCTGTTGGAGCGCTGGAGGGAGCTAATTTCTTGTCAACTGGAAATCTTGTTAGTTTGAGCGAGCAACAGCTTGTGGATTGTGATCACGAGGTTTGCTCCATTCCTTCCCTGCCTTGGATTTATTGCCTTTTGTTCGTATTCATAATTTGTTGATCCACTTCTCTCTCTCTTTAACCTGCTACAGAGGTTCGGTTTATAACTTTTCATTTTTAGAAATTATGCTCGTTTACTCATACTTTTTTTTTTAGGAAAATGTTTTCGAATCTACTGTTCTTTTAGCTTAAATTATTAAAAATACAATGAAAATTTTAAGAGTTTTATTTTAGCCTAAAAATTTGTTTCAAAACAATCTTTGAAGATTTTTTTTTGTTAAATTCATAGTAGGAAAATGACTGGCATATGTATTATTGGTTATATGAACAAATTTGAATACATACCTCCAAAGAACAATAAAAACACATTTGCACGTCATTTTTTGTCCATAAATTTAACTGAACATGTCTCTAAGAGTAGTTTTGAAATAATTTCAAATTTCATAGGTTTAAATGAAAATTTTGAAAGTTTAGGAGTATAATTGAAATCAGAGTATTTTCAATTATTTAAGCGATCTTTTATATTTGAAATTTGACTAGATTTCAAAAGTTAATGTCCAGAAACAAGAAAATGATGTGTATATATATTTTGCAAAGTCAATGAAATGATATATCCTATCCATTAACAAATTTGTTTGGTCTCACTGAGCACAGCACCCATCTATTCAGGTCATGTTCAAAATTTTTTTGTTGCTTTGCAACTAAGTAATGATTTCCTACTCTCTGTTTCAGTTTATTTCTAGTGAATACAATATGTTAGTGAAATTCAATTATTTATTAAAGTAAAAGAGAAATCTTAGAAGGAAATTTTTGTTCATAACTTTATAAACCATATATCATTTTGTCGATCTAATGGGAAATGAATAGTTTATTATTATACCTTTTCGAAAAAAAATGGATTATTATTTTTTAAGAAAGGAAGGGTGTGTTTAGTTGGTGATTTGAAAACAAAAATTTGAAAACAATGTTTTAGCAGATTCAGAAATTGGATCCCAATTTAAATAATTGTTCAAAATGTGTTTTGATAATATATTTATGAATCATAAAGTATTTGTAGTTATCAATTAAACACTAGTTTGAATATGAGCTATTATTAATTAATTTATAAACATGTTATAAATTTTTTATAATTATTATTCTACAATATCATATAATTTATAATATATTATATTATACAAATTTTAATTTCACATTTTTTTTTATAGTTTATAACATGAAATACTATATTTATTAAAAATTTTATCTTTTGTAATTTAATTTTGCATTTAAAAATTTTAAATTCAAAATCTTTGATACAATGAAAACATAAAAAAAGTTGTTTTTAGAATTTTCACTATTTAAACCATTATAGGTTGGCCTTAGTGGTCAATAAGGGTCATAGGGCTTAGAGGGAATAGGTTCAATCTACAATGATTGTCCTGTGAGATTAGTCAGGTCAGGTGCGTGTAAGTTGGCTCGGACACTCACGAATATGAAAAAAAAAACACTATTTGGATCAGAGAATCCGAAAACAAAACAGTTTTAAAAAACACATCTGTCTAAAACTTATTCACTAATAAAACATAAAAACAGAATTTGGATTGTTTACCAAACAGATCCAAAATATCTGAAGAAATATATAAATGAGAAGAAATATATGATGAGATTACAAGATTTTATTCTATGTTATTTTATTTTTGAATACAGATGATTTGTATCTGTATATATATTGAACACAACATACTTGCAAAATATTAATAACACGTTTTGTTCGTAAAAGAAACTTGGTTTGTCAAATTATTTTTTATTTTTTTTATTATTATTTTTCTTTTTAAGAAATAACTACTTTGGCCTTTAGTTACTGTACTTTTTGTTTGTTTTCTATTTTCTAAAAATAAATTTGAAAATTTCACTAATTATTCCTTTTAATATTTAAAATTGAATTAAAATTTAAAAATGATTTTAAAACATAAACTACATAAAATGGTTTGGTTATTTTTTATTTTTTATTTTATTTTTTGAGAAAGTAAATGGTTTGGTTATAAACTATGTTTTTCTTTTTTTAAATAACTGGGGTGAGAGCATACAAACCATGACCGAGGACATATACTTTATGTCTATTGAGCTATATTCTGTTTGACAATTATAAACTATGTTTATTTTTATAAGACATTTATAATACATAAATTATATTTTCCAACACAATCATTCATTTGTTGAATTCTCAAATCCTATTAAAGCCAACCTTGTTATAGATACTAATACTTTGATGAATTGCAAGAAAAAGTTAAAGTGACCCCAAAATGTCATGACACTTTTCGTGAAATTAATTAAACTCCCAAAAATGACTTAGTTTAATTTGGTTGTCGTAACAATTCATCATGAATTGAACGATAGTTATGTAGATTGATTAGATTAAAACACCTAATGTATTTAATGTTAGCTTCAACTCTCCACCTAAAGATAGTACACCAATTTTTAAAGGAAAATACTTCCGGCCGATTGATTAGTAGAATTTTTTTTTGACTTAGCTCTATATTTTTTTAGTGAAAAATACTCTCTTGTTGACATGTCAGTAGGAATTTCTAGTAATCACCCCTAGGCTCTAAGTTTTTTTGTTTCTTGTGTTATTTGTCTCTGTCTTTGTCTTTTTTTTTTGTCTTTTTTTTTTTAAAATTTATTTAAAGAAAAAGTCTATCTTTTATTTTAGGATGTATGGTTCTTTTTTTAAAAACAGAATCATAGCTAGGGCTCTTAGAACCTAGAAAATGAGGACAAGAATATTCTCACGTTTGGTTTAGAACTTTCACCCATTTACCACCAAATTTTAGCATAGATCAATTATTTATAATGAAAACTCCAAAAGAAATAGTAATAGGTTTTATGATATTTTGTTTGTCTTAGATTGGCTATGATATTGCTTGAACTTAAAACTTATCAATCAATAATCTACTTCCAAGTTTCAATAACGACACACTCGTATTTTCATCTTAAAGGTAGATGGTTACGATAAATTAGTTTGGAGAGGTTACTGATTTATGTCATGAATTAATTGAAAATAAAATGAATGTGTTTTGCAGTGTGATCCTGAGGAAGCTGGTGCATGCGACGCGGGGTGCGGCGGGGGACTAATGAATTCAGCTTTCGAGTACATTTTGAAATCAGGTGGATTAGAGCGAGAAGAGGACTATCCTTACACTGGGACGGATCGTGGCCCTTGCAAGTTCCAAAATGGCAAAATCGCTGCCTCTGTAGCCAACTTCAGCGTTGTTTCTGAAGATGCCGACCAAATTGCAGCAAACTTGGTCAAGAATGGCCCTTTAGCAGGTAATGAAATTCATTCTTCTTCACTTTTTAAGTGCGTTTTATGACTTCTCAACCCGATTTTAAACCCGTAATGATGTGAAAAACTGAAGCTATCAGGATAGTATATATATATATATATATATTTTAAAAAATAATTATATGCTTATTTCCTTGCCTTTTATTTATTTATTTATTTTTAATATTGTTTCCCTTTGTTAAAGAACACATATCATATAGCTTTCAAAGTATAGTTTTCAATTTGAAAACATTTTTTTAGAAGAATATAACCAGACAAAGAAAAATATAGGTAGAGGTGATATTTATAGCCTTAATTTTTGAAAATAGAAAACGAAAATAAAAAAGATTATCTAATGAGCTAATATATGCGTTCTTGCATGTTTTTTTTTTTTTTTTGAGTTCAACAACATTGGAATGGGGGGATTCGAACCTCTGACCTCTTGATTGAAAATACATGTCAATTACCACTAAACTATGTCCACTTTGGCGCAGATGTCATTATGTTATACCAATTATTTCCTTTTTGTTTATTTTAATTATGGTGAATTCTTCTCTTATGGCTATAGTTTCTTAATTTTCCACGTGAATCTTTGACTATCAACTTTATTGCTCCCATTTGCCATATATTATTGTCAATTTGCAAGAGCTGTGTTTTTTTTTCAATTCTATTCTACGTGAATTTCAAACATCCTTCTGTTGCCCATTCCCCCATGTGCTTTGAACCTTCATCCTTTATCGAGAGTTTCTAGGTTTTCCACAAAAATCCATATATTCTATTTCTGTAGATTTTAGTTGGAAGAGCCATTAATTATTACTGTTTTTCTATTTTCAAAGTAATCGGAGCAACGAAAGACTGAATCTCAATGAATCGTGGTAGCAAAGCTTCCATTATTGCTGCTTTCAACCCCCAAGTTTCTAACATTCATCCCTTTGCCTATTCCCCCGCAGTTGGAATCAACGCAGTTTTCATGCAGACCTACATAGGGGGAGTTTCATGCCCATACATATGCTCTAAACGCAACCTAGATCATGGAGTACTGATTGTAGGGTATGGATCTGCTGGCTTTGCTCCGATCCGGTTCAAGGAGAAGCCCTACTGGATCATAAAGAACTCTTGGGGTGAAAACTGGGGAGAAAATGGATACTACTTCATTTGCAAGGGCAAAAATATCTGTGGAGTCGAGTCGATGGTCTCGTCCGTGGCCGCTACCAAGAACTGAGCCATTCTTAGCGCTTTAATATCAAGGTAAGTTGCTGGGGCTCTGTAAATATGTTATATTCTGGGAAATGGTACGTTACATCAAGCTCTTGTGGAACTTGGTCATTCTCTTGGCTGATGGTAGATATTGCTAGTTACTGTTGTATAAGTTGTATGTTGGAATAATGTTGCACTCTGTAAGCTTTTGGATTCTAGAAATGCAGACATTTTTATACTATCTGTTTTTTTTTTTTACTTCGTCAACATTTGGAAGTGGGGAGATTCGAATCTCTGACCTCT

mRNA sequence

ATGGAGCGCCTCACTGTAATCGCCTTCTTCTTTATCATTCTACTATCGACGACGGTAGCCTACGGCGTATCTTCCGATCAAATCAACGGCGCCAACGAAGACGATCTTCTGATTCGTCAAGTCGTGTCCGGTGCCGATGATCGTCTCTTAACCGCCGAGCAACACTTCCAGAACTTCAAACTCAAGTTCGGAAAGACCTACGCCACTGATGAGGAGCACGATTACAGGTTCCGTGTCTTCAAGGCTAACCTTCGCAGAGCCAAACGCCACCAGAAGCTCGATCCCGGCGCCGTCCATGGCGTCACTCGGTTTTCGGATCTCACCGAGTCCGAGTTCCGTAAAAATTTTGTTGGAGTGAATCGGCTCCGTCTTCCTGCCGATGCTCACGAGGCTCCGATTCTTCCTACCGATGACCTTGCCTCCGACTTCGATTGGCGCAACCTCGGTGCCGTCACGCCAGTCAAAGATCAGGGTTCTTGTGGCTCGTGCTGGTCTTTCAGTGCTGTTGGAGCGCTGGAGGGAGCTAATTTCTTGTCAACTGGAAATCTTGTTAGTTTGAGCGAGCAACAGCTTGTGGATTGTGATCACGAGTGTGATCCTGAGGAAGCTGGTGCATGCGACGCGGGGTGCGGCGGGGGACTAATGAATTCAGCTTTCGAGTACATTTTGAAATCAGGTGGATTAGAGCGAGAAGAGGACTATCCTTACACTGGGACGGATCGTGGCCCTTGCAAGTTCCAAAATGGCAAAATCGCTGCCTCTGTAGCCAACTTCAGCGTTGTTTCTGAAGATGCCGACCAAATTGCAGCAAACTTGGTCAAGAATGGCCCTTTAGCAGTTGGAATCAACGCAGTTTTCATGCAGACCTACATAGGGGGAGTTTCATGCCCATACATATGCTCTAAACGCAACCTAGATCATGGAGTACTGATTGTAGGGTATGGATCTGCTGGCTTTGCTCCGATCCGGTTCAAGGAGAAGCCCTACTGGATCATAAAGAACTCTTGGGGTGAAAACTGGGGAGAAAATGGATACTACTTCATTTGCAAGGGCAAAAATATCTGTGGAGTCGAGTCGATGGTCTCGTCCGTGGCCGCTACCAAGAACTGA

Coding sequence (CDS)

ATGGAGCGCCTCACTGTAATCGCCTTCTTCTTTATCATTCTACTATCGACGACGGTAGCCTACGGCGTATCTTCCGATCAAATCAACGGCGCCAACGAAGACGATCTTCTGATTCGTCAAGTCGTGTCCGGTGCCGATGATCGTCTCTTAACCGCCGAGCAACACTTCCAGAACTTCAAACTCAAGTTCGGAAAGACCTACGCCACTGATGAGGAGCACGATTACAGGTTCCGTGTCTTCAAGGCTAACCTTCGCAGAGCCAAACGCCACCAGAAGCTCGATCCCGGCGCCGTCCATGGCGTCACTCGGTTTTCGGATCTCACCGAGTCCGAGTTCCGTAAAAATTTTGTTGGAGTGAATCGGCTCCGTCTTCCTGCCGATGCTCACGAGGCTCCGATTCTTCCTACCGATGACCTTGCCTCCGACTTCGATTGGCGCAACCTCGGTGCCGTCACGCCAGTCAAAGATCAGGGTTCTTGTGGCTCGTGCTGGTCTTTCAGTGCTGTTGGAGCGCTGGAGGGAGCTAATTTCTTGTCAACTGGAAATCTTGTTAGTTTGAGCGAGCAACAGCTTGTGGATTGTGATCACGAGTGTGATCCTGAGGAAGCTGGTGCATGCGACGCGGGGTGCGGCGGGGGACTAATGAATTCAGCTTTCGAGTACATTTTGAAATCAGGTGGATTAGAGCGAGAAGAGGACTATCCTTACACTGGGACGGATCGTGGCCCTTGCAAGTTCCAAAATGGCAAAATCGCTGCCTCTGTAGCCAACTTCAGCGTTGTTTCTGAAGATGCCGACCAAATTGCAGCAAACTTGGTCAAGAATGGCCCTTTAGCAGTTGGAATCAACGCAGTTTTCATGCAGACCTACATAGGGGGAGTTTCATGCCCATACATATGCTCTAAACGCAACCTAGATCATGGAGTACTGATTGTAGGGTATGGATCTGCTGGCTTTGCTCCGATCCGGTTCAAGGAGAAGCCCTACTGGATCATAAAGAACTCTTGGGGTGAAAACTGGGGAGAAAATGGATACTACTTCATTTGCAAGGGCAAAAATATCTGTGGAGTCGAGTCGATGGTCTCGTCCGTGGCCGCTACCAAGAACTGA

Protein sequence

MERLTVIAFFFIILLSTTVAYGVSSDQINGANEDDLLIRQVVSGADDRLLTAEQHFQNFKLKFGKTYATDEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGVNRLRLPADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLSTGNLVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGTDRGPCKFQNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYICSKRNLDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVESMVSSVAATKN
Homology
BLAST of Spg033738 vs. NCBI nr
Match: XP_022951324.1 (cysteine protease RD19A-like [Cucurbita moschata] >XP_022951325.1 cysteine protease RD19A-like [Cucurbita moschata])

HSP 1 Score: 697.2 bits (1798), Expect = 7.6e-197
Identity = 333/369 (90.24%), Postives = 352/369 (95.39%), Query Frame = 0

Query: 1   MERLTVIAFFFIILLSTTVAYGVSSDQINGANEDDLLIRQVVSGADDRLLTAEQHFQNFK 60
           MERLTVIA  F ILLS TVAYG SSDQINGA +DD+LIRQVVSGADDRLLTAEQHFQNFK
Sbjct: 1   MERLTVIALSFAILLSATVAYGESSDQINGAEDDDILIRQVVSGADDRLLTAEQHFQNFK 60

Query: 61  LKFGKTYATDEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGVN 120
           LKFGKTYATDEEHDYRFR+FKANLR+AKRHQKLDPGAVHGVT+FSDLTE EFRKNFVGVN
Sbjct: 61  LKFGKTYATDEEHDYRFRIFKANLRKAKRHQKLDPGAVHGVTQFSDLTEDEFRKNFVGVN 120

Query: 121 RLRLPADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLST 180
           RLRLPADA +APILPTD+LASDFDWR+ GAVTPVK+QGSCGSCWSFSAVGALEGANFLST
Sbjct: 121 RLRLPADAQKAPILPTDNLASDFDWRDHGAVTPVKNQGSCGSCWSFSAVGALEGANFLST 180

Query: 181 GNLVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGTD 240
           G LVSLSEQQLVDCDHECDPEEAGACDAGC GGLMNSAFEYILK+GGLEREEDYPYTG D
Sbjct: 181 GQLVSLSEQQLVDCDHECDPEEAGACDAGCNGGLMNSAFEYILKAGGLEREEDYPYTGKD 240

Query: 241 RGPCKFQNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYIC 300
           RGPCKFQN KIAASV+NFSV+S DADQIAANL+KNGPLA+GINA FMQTY+ GVSCPYIC
Sbjct: 241 RGPCKFQNAKIAASVSNFSVISLDADQIAANLLKNGPLAIGINAAFMQTYMKGVSCPYIC 300

Query: 301 SKRNLDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVESM 360
           SKRNL+HGVL+VGYGSAG+APIRFKEKPYWIIKNSWGENWGENGYY ICKGKNICGVESM
Sbjct: 301 SKRNLNHGVLLVGYGSAGYAPIRFKEKPYWIIKNSWGENWGENGYYLICKGKNICGVESM 360

Query: 361 VSSVAATKN 370
           VSSVAAT+N
Sbjct: 361 VSSVAATQN 369

BLAST of Spg033738 vs. NCBI nr
Match: XP_023002391.1 (cysteine protease RD19A-like [Cucurbita maxima])

HSP 1 Score: 693.7 bits (1789), Expect = 8.4e-196
Identity = 331/369 (89.70%), Postives = 350/369 (94.85%), Query Frame = 0

Query: 1   MERLTVIAFFFIILLSTTVAYGVSSDQINGANEDDLLIRQVVSGADDRLLTAEQHFQNFK 60
           MERLTVIA  F ILLS TVAYGVSSDQING  +DDLLIRQVVSG DDRLLTAEQHFQNFK
Sbjct: 1   MERLTVIALSFAILLSATVAYGVSSDQINGVEDDDLLIRQVVSGDDDRLLTAEQHFQNFK 60

Query: 61  LKFGKTYATDEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGVN 120
           LKFGKTYATDEEHDYRFR+FKANLR+AKRHQKLDPGA+HGVT+FSDLTE EFRKNFVGVN
Sbjct: 61  LKFGKTYATDEEHDYRFRIFKANLRKAKRHQKLDPGAIHGVTQFSDLTEDEFRKNFVGVN 120

Query: 121 RLRLPADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLST 180
           RLRLPADA +APILPTD+LASDFDWR+ GAVTPVK+QGSCGSCWSFSAVGALEGANFLST
Sbjct: 121 RLRLPADAQKAPILPTDNLASDFDWRDHGAVTPVKNQGSCGSCWSFSAVGALEGANFLST 180

Query: 181 GNLVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGTD 240
           G LVSLSEQQLVDCDHECDPEEAGACDAGC GGLM SAFEYILK+GGLEREEDYPYTG D
Sbjct: 181 GKLVSLSEQQLVDCDHECDPEEAGACDAGCNGGLMTSAFEYILKAGGLEREEDYPYTGKD 240

Query: 241 RGPCKFQNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYIC 300
           RGPCKFQN KIAASV+NFSV+S DADQIAANL+KNGPLA+GINA FMQTY+ GVSCPYIC
Sbjct: 241 RGPCKFQNAKIAASVSNFSVISLDADQIAANLLKNGPLAIGINAAFMQTYMKGVSCPYIC 300

Query: 301 SKRNLDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVESM 360
           SKRNL+HGVL+VGYGSAG+APIRFKEKPYWIIKNSWGENWGENGYY ICKGKNICGVESM
Sbjct: 301 SKRNLNHGVLLVGYGSAGYAPIRFKEKPYWIIKNSWGENWGENGYYLICKGKNICGVESM 360

Query: 361 VSSVAATKN 370
           VSSVAAT+N
Sbjct: 361 VSSVAATQN 369

BLAST of Spg033738 vs. NCBI nr
Match: XP_023537303.1 (cysteine protease RD19A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 693.3 bits (1788), Expect = 1.1e-195
Identity = 332/366 (90.71%), Postives = 349/366 (95.36%), Query Frame = 0

Query: 1   MERLTVIAFFFIILLSTTVAYGVSSDQINGANEDDLLIRQVVSGADDRLLTAEQHFQNFK 60
           MERLTVIA  F ILLS TVAYG SSDQINGA +DDLLIRQVVSGADDRLLTAEQHFQNFK
Sbjct: 1   MERLTVIALSFAILLSATVAYGESSDQINGAEDDDLLIRQVVSGADDRLLTAEQHFQNFK 60

Query: 61  LKFGKTYATDEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGVN 120
           LKFGKTYATDEEHDYR R+FKANLR+AKRHQKLDPGAVHGVT+FSDLTE EFRKNFVGVN
Sbjct: 61  LKFGKTYATDEEHDYRLRIFKANLRKAKRHQKLDPGAVHGVTQFSDLTEDEFRKNFVGVN 120

Query: 121 RLRLPADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLST 180
           RLRLPADA +APILPTD+LASDFDWR+ GAVTPVK+QGSCGSCWSFSAVGALEGANFLST
Sbjct: 121 RLRLPADAQKAPILPTDNLASDFDWRDHGAVTPVKNQGSCGSCWSFSAVGALEGANFLST 180

Query: 181 GNLVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGTD 240
           GNLVSLSEQQLVDCDHECDPEEAGACDAGC GGLMNSAFEYILK+GGLEREEDYPYTG D
Sbjct: 181 GNLVSLSEQQLVDCDHECDPEEAGACDAGCNGGLMNSAFEYILKAGGLEREEDYPYTGKD 240

Query: 241 RGPCKFQNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYIC 300
           RGPCKFQN KIAASV+NFSV+S DADQIAANL+KNGPLA+GINA FMQTY+ GVSCPYIC
Sbjct: 241 RGPCKFQNAKIAASVSNFSVISLDADQIAANLLKNGPLAIGINAAFMQTYMKGVSCPYIC 300

Query: 301 SKRNLDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVESM 360
           SKRNL+HGVL+VGYGSAG+APIRFKEKPYWIIKNSWGENWGENGYY ICKGKNICGVESM
Sbjct: 301 SKRNLNHGVLLVGYGSAGYAPIRFKEKPYWIIKNSWGENWGENGYYLICKGKNICGVESM 360

Query: 361 VSSVAA 367
           VSSVAA
Sbjct: 361 VSSVAA 366

BLAST of Spg033738 vs. NCBI nr
Match: XP_038884968.1 (cysteine protease RD19A-like [Benincasa hispida])

HSP 1 Score: 693.0 bits (1787), Expect = 1.4e-195
Identity = 332/371 (89.49%), Postives = 348/371 (93.80%), Query Frame = 0

Query: 1   MERLTVIAFFFIILLSTTVAYGVSSDQINGANED--DLLIRQVVSGADDRLLTAEQHFQN 60
           M+RL V A FF ILLS TV YGVSSDQINGA  D  DLLIRQVVSGADDR LTAEQHFQN
Sbjct: 1   MDRLAVFALFFTILLSATVTYGVSSDQINGAQSDDEDLLIRQVVSGADDRRLTAEQHFQN 60

Query: 61  FKLKFGKTYATDEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVG 120
           FKLKFGKTYATDEEHDYRFR+FKANLR+AKRHQKLDP AVHGVTRFSDLTESEFR NFVG
Sbjct: 61  FKLKFGKTYATDEEHDYRFRIFKANLRKAKRHQKLDPDAVHGVTRFSDLTESEFRTNFVG 120

Query: 121 VNRLRLPADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFL 180
           +NRLRLPADA +APILPTDDLA DFDWR+ GAVTPVKDQGSCGSCWSFSAVGA+EGANFL
Sbjct: 121 LNRLRLPADAQKAPILPTDDLAPDFDWRDQGAVTPVKDQGSCGSCWSFSAVGAVEGANFL 180

Query: 181 STGNLVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTG 240
           STG LVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAF+YILK+GGLE+EEDYPYTG
Sbjct: 181 STGKLVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFQYILKAGGLEKEEDYPYTG 240

Query: 241 TDRGPCKFQNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPY 300
           TDRGPCKFQN K+AASVANFSV+S DADQIAANLVKNGPLA+GINAVFMQTYI GVSCPY
Sbjct: 241 TDRGPCKFQNSKVAASVANFSVISNDADQIAANLVKNGPLAIGINAVFMQTYIKGVSCPY 300

Query: 301 ICSKRNLDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVE 360
           ICSKRNLDHGVL+VGYGSAGFAPIRFKEKPYWIIKNSWG+NWGENGYY ICKG+NICG E
Sbjct: 301 ICSKRNLDHGVLLVGYGSAGFAPIRFKEKPYWIIKNSWGQNWGENGYYLICKGRNICGSE 360

Query: 361 SMVSSVAATKN 370
           SMVSSVAAT N
Sbjct: 361 SMVSSVAATPN 371

BLAST of Spg033738 vs. NCBI nr
Match: KAG6585563.1 (Cysteine protease RD19A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 691.8 bits (1784), Expect = 3.2e-195
Identity = 331/366 (90.44%), Postives = 348/366 (95.08%), Query Frame = 0

Query: 1   MERLTVIAFFFIILLSTTVAYGVSSDQINGANEDDLLIRQVVSGADDRLLTAEQHFQNFK 60
           MERLTVIA  F ILLS TV YG SSDQINGA +DDLLIRQVVSGADDRLLTAEQHFQNFK
Sbjct: 1   MERLTVIALSFAILLSATVGYGESSDQINGAEDDDLLIRQVVSGADDRLLTAEQHFQNFK 60

Query: 61  LKFGKTYATDEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGVN 120
           LKFGKTYATDEEHDYRFR+FKANLR+AKRHQKLDPGAVHGVT+FSDLTE EFRKNFVGVN
Sbjct: 61  LKFGKTYATDEEHDYRFRIFKANLRKAKRHQKLDPGAVHGVTQFSDLTEDEFRKNFVGVN 120

Query: 121 RLRLPADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLST 180
           RLRLPADA +APILPTD+LASDFDWR+ GAVTPVK+QGSCGSCWSFSAVGALEGANFLST
Sbjct: 121 RLRLPADAQKAPILPTDNLASDFDWRDHGAVTPVKNQGSCGSCWSFSAVGALEGANFLST 180

Query: 181 GNLVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGTD 240
           G LVSLSEQQLVDCDHECDPEEAGACDAGC GGLMNSAFEYILK+GGLEREEDYPYTG D
Sbjct: 181 GQLVSLSEQQLVDCDHECDPEEAGACDAGCNGGLMNSAFEYILKAGGLEREEDYPYTGKD 240

Query: 241 RGPCKFQNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYIC 300
           RGPCKFQN KIAASV+NFSV+S DADQIAANL+KNGPLA+GINA FMQTY+ GVSCPYIC
Sbjct: 241 RGPCKFQNAKIAASVSNFSVISLDADQIAANLLKNGPLAIGINAAFMQTYMKGVSCPYIC 300

Query: 301 SKRNLDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVESM 360
           SKRNL+HGVL+VGYGSAG+APIRFKEKPYWIIKNSWGENWGENGYY ICKGKNICGVESM
Sbjct: 301 SKRNLNHGVLLVGYGSAGYAPIRFKEKPYWIIKNSWGENWGENGYYLICKGKNICGVESM 360

Query: 361 VSSVAA 367
           VSSVAA
Sbjct: 361 VSSVAA 366

BLAST of Spg033738 vs. ExPASy Swiss-Prot
Match: P43296 (Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 6.3e-154
Identity = 265/368 (72.01%), Postives = 310/368 (84.24%), Query Frame = 0

Query: 1   MERLTVIAFFFIILLSTTVAYGVSSDQINGANEDDLLIRQVVSGADDRLLTAEQHFQNFK 60
           M+RL +  +F + +LS  +   VSS  +N  + DDL+IRQVV GA+ ++LT+E HF  FK
Sbjct: 1   MDRLKL--YFSVFVLSFFIV-SVSSSDVN--DGDDLVIRQVVGGAEPQVLTSEDHFSLFK 60

Query: 61  LKFGKTYATDEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGV- 120
            KFGK YA++EEHDYRF VFKANLRRA+RHQKLDP A HGVT+FSDLT SEFRK  +GV 
Sbjct: 61  RKFGKVYASNEEHDYRFSVFKANLRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHLGVR 120

Query: 121 NRLRLPADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLS 180
           +  +LP DA++APILPT++L  DFDWR+ GAVTPVK+QGSCGSCWSFSA GALEGANFL+
Sbjct: 121 SGFKLPKDANKAPILPTENLPEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGANFLA 180

Query: 181 TGNLVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGT 240
           TG LVSLSEQQLVDCDHECDPEEA +CD+GC GGLMNSAFEY LK+GGL +EEDYPYTG 
Sbjct: 181 TGKLVSLSEQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKTGGLMKEEDYPYTGK 240

Query: 241 DRGPCKFQNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYI 300
           D   CK    KI ASV+NFSV+S D +QIAANLVKNGPLAV INA +MQTYIGGVSCPYI
Sbjct: 241 DGKTCKLDKSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVSCPYI 300

Query: 301 CSKRNLDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVES 360
           C++R L+HGVL+VGYG+AG+AP RFKEKPYWIIKNSWGE WGENG+Y ICKG+NICGV+S
Sbjct: 301 CTRR-LNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICGVDS 360

Query: 361 MVSSVAAT 368
           MVS+VAAT
Sbjct: 361 MVSTVAAT 362

BLAST of Spg033738 vs. ExPASy Swiss-Prot
Match: P25804 (Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 4.9e-146
Identity = 244/362 (67.40%), Postives = 290/362 (80.11%), Query Frame = 0

Query: 9   FFFIILLSTTVAYGVSSDQINGANEDDLLIRQVVSGADDRLLTAEQHFQNFKLKFGKTYA 68
           F F + L   VA  V+ D     N DD +IRQVV   +D LL AE HF +FK KF K+YA
Sbjct: 5   FLFALFLFAAVATAVTDD----TNNDDFIIRQVVDNEEDHLLNAEHHFTSFKSKFSKSYA 64

Query: 69  TDEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGV-NRLRLPAD 128
           T EEHDYRF VFK+NL +AK HQ  DP A HG+T+FSDLT SEFR+ F+G+  RLRLPA 
Sbjct: 65  TKEEHDYRFGVFKSNLIKAKLHQNRDPTAEHGITKFSDLTASEFRRQFLGLKKRLRLPAH 124

Query: 129 AHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLSTGNLVSLS 188
           A +APILPT +L  DFDWR  GAVTPVKDQGSCGSCW+FS  GALEGA++L+TG LVSLS
Sbjct: 125 AQKAPILPTTNLPEDFDWREKGAVTPVKDQGSCGSCWAFSTTGALEGAHYLATGKLVSLS 184

Query: 189 EQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGTDRGPCKFQ 248
           EQQLVDCDH CDPE+AG+CD+GC GGLMN+AFEY+L+SGG+ +E+DY YTG D G CKF 
Sbjct: 185 EQQLVDCDHVCDPEQAGSCDSGCNGGLMNNAFEYLLESGGVVQEKDYAYTGRD-GSCKFD 244

Query: 249 NGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYICSKRNLDH 308
             K+ ASV+NFSVV+ D DQIAANLVKNGPLAV INA +MQTY+ GVSCPY+C+K  LDH
Sbjct: 245 KSKVVASVSNFSVVTLDEDQIAANLVKNGPLAVAINAAWMQTYMSGVSCPYVCAKSRLDH 304

Query: 309 GVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVESMVSSVAAT 368
           GVL+VG+G   +APIR KEKPYWIIKNSWG+NWGE GYY IC+G+N+CGV+SMVS+VAA 
Sbjct: 305 GVLLVGFGKGAYAPIRLKEKPYWIIKNSWGQNWGEQGYYKICRGRNVCGVDSMVSTVAAA 361

Query: 369 KN 370
           ++
Sbjct: 365 QS 361

BLAST of Spg033738 vs. ExPASy Swiss-Prot
Match: P43295 (Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 SV=2)

HSP 1 Score: 518.5 bits (1334), Expect = 6.4e-146
Identity = 248/356 (69.66%), Postives = 293/356 (82.30%), Query Frame = 0

Query: 13  ILLSTTVAYGVSSDQINGANEDDLLIRQVVSGADDRLLTAEQHFQNFKLKFGKTYATDEE 72
           +L S ++ +   S  + G  ++D+LIRQVV   + ++L++E HF  FK KFGK Y + EE
Sbjct: 7   VLFSVSLIFVFVSVSVCG--DEDVLIRQVVDETEPKVLSSEDHFTLFKKKFGKVYGSIEE 66

Query: 73  HDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGV-NRLRLPADAHEA 132
           H YRF VFKANL RA RHQK+DP A HGVT+FSDLT SEFR+  +GV    +LP DA++A
Sbjct: 67  HYYRFSVFKANLLRAMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGVKGGFKLPKDANQA 126

Query: 133 PILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLSTGNLVSLSEQQL 192
           PILPT +L  +FDWR+ GAVTPVK+QGSCGSCWSFS  GALEGA+FL+TG LVSLSEQQL
Sbjct: 127 PILPTQNLPEEFDWRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFLATGKLVSLSEQQL 186

Query: 193 VDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGTDRGPCKFQNGKI 252
           VDCDHECDPEE G+CD+GC GGLMNSAFEY LK+GGL RE+DYPYTGTD G CK    KI
Sbjct: 187 VDCDHECDPEEEGSCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTGTDGGSCKLDRSKI 246

Query: 253 AASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYICSKRNLDHGVLI 312
            ASV+NFSVVS + DQIAANL+KNGPLAV INA +MQTYIGGVSCPYICS+R L+HGVL+
Sbjct: 247 VASVSNFSVVSINEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPYICSRR-LNHGVLL 306

Query: 313 VGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVESMVSSVAAT 368
           VGYGSAGF+  R KEKPYWIIKNSWGE+WGENG+Y ICKG+NICGV+S+VS+VAAT
Sbjct: 307 VGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDSLVSTVAAT 359

BLAST of Spg033738 vs. ExPASy Swiss-Prot
Match: Q9SUL1 (Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 6.2e-141
Identity = 244/363 (67.22%), Postives = 292/363 (80.44%), Query Frame = 0

Query: 10  FFIILLSTTVAYGVSSDQINGANEDDLL--IRQVV-SGADDRLLTAEQHFQNFKLKFGKT 69
           FF ++ +T +A  + S  I+G   D  +  IRQVV    D++LL AE HF  FK K+ KT
Sbjct: 6   FFFLIAATLLAGSLGSTVISGEVTDGFVNPIRQVVPEENDEQLLNAEHHFTLFKSKYEKT 65

Query: 70  YATDEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGVNR--LRL 129
           YAT  EHD+RFRVFKANLRRA+R+Q LDP AVHGVT+FSDLT  EFR+ F+G+ R   RL
Sbjct: 66  YATQVEHDHRFRVFKANLRRARRNQLLDPSAVHGVTQFSDLTPKEFRRKFLGLKRRGFRL 125

Query: 130 PADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLSTGNLV 189
           P D   APILPT DL ++FDWR  GAVTPVK+QG CGSCWSFSA+GALEGA+FL+T  LV
Sbjct: 126 PTDTQTAPILPTSDLPTEFDWREQGAVTPVKNQGMCGSCWSFSAIGALEGAHFLATKELV 185

Query: 190 SLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGTDRGPC 249
           SLSEQQLVDCDHECDP +A +CD+GC GGLMN+AFEY LK+GGL +EEDYPYTG D   C
Sbjct: 186 SLSEQQLVDCDHECDPAQANSCDSGCSGGLMNNAFEYALKAGGLMKEEDYPYTGRDHTAC 245

Query: 250 KFQNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYICSKRN 309
           KF   KI ASV+NFSVVS D DQIAANLV++GPLA+ INA++MQTYIGGVSCPY+CSK +
Sbjct: 246 KFDKSKIVASVSNFSVVSSDEDQIAANLVQHGPLAIAINAMWMQTYIGGVSCPYVCSK-S 305

Query: 310 LDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKG-KNICGVESMVSS 367
            DHGVL+VG+GS+G+APIR KEKPYWIIKNSWG  WGE+GYY IC+G  N+CG+++MVS+
Sbjct: 306 QDHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGYYKICRGPHNMCGMDTMVST 365

BLAST of Spg033738 vs. ExPASy Swiss-Prot
Match: Q10716 (Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 9.5e-134
Identity = 235/366 (64.21%), Postives = 281/366 (76.78%), Query Frame = 0

Query: 12  IILLSTTVAYGVSSDQINGANEDDLLIRQVVSGADDR--LLTAEQHFQNFKLKFGKTYAT 71
           ++LLS   A  V++      + +D LIRQVV G DD    L AE HF +F  +FGK+Y  
Sbjct: 6   LLLLSLASAAAVAA----AVDAEDPLIRQVVPGGDDNDLELNAESHFLSFVQRFGKSYKD 65

Query: 72  DEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGVNRLR------ 131
            +EH YR  VFK NLRRA+RHQ LDP A HGVT+FSDLT +EFR+ ++G+ + R      
Sbjct: 66  ADEHAYRLSVFKDNLRRARRHQLLDPSAEHGVTKFSDLTPAEFRRTYLGLRKSRRALLRE 125

Query: 132 LPADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLSTGNL 191
           L   AHEAP+LPTD L  DFDWR+ GAV PVK+QGSCGSCWSFSA GALEGA++L+TG L
Sbjct: 126 LGESAHEAPVLPTDGLPDDFDWRDHGAVGPVKNQGSCGSCWSFSASGALEGAHYLATGKL 185

Query: 192 VSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGTDRGP 251
             LSEQQ VDCDHECD  E  +CD+GC GGLM +AF Y+ K+GGLE E+DYPYTG+D G 
Sbjct: 186 EVLSEQQFVDCDHECDSSEPDSCDSGCNGGLMTTAFSYLQKAGGLESEKDYPYTGSD-GK 245

Query: 252 CKFQNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYICSKR 311
           CKF   KI ASV NFSVVS D  QI+ANL+K+GPLA+GINA +MQTYIGGVSCPYIC  R
Sbjct: 246 CKFDKSKIVASVQNFSVVSVDEAQISANLIKHGPLAIGINAAYMQTYIGGVSCPYICG-R 305

Query: 312 NLDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNI---CGVESM 367
           +LDHGVL+VGYG++GFAPIR K+KPYWIIKNSWGENWGENGYY IC+G N+   CGV+SM
Sbjct: 306 HLDHGVLLVGYGASGFAPIRLKDKPYWIIKNSWGENWGENGYYKICRGSNVRNKCGVDSM 365

BLAST of Spg033738 vs. ExPASy TrEMBL
Match: A0A6J1GIG1 (cysteine protease RD19A-like OS=Cucurbita moschata OX=3662 GN=LOC111454190 PE=3 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 3.7e-197
Identity = 333/369 (90.24%), Postives = 352/369 (95.39%), Query Frame = 0

Query: 1   MERLTVIAFFFIILLSTTVAYGVSSDQINGANEDDLLIRQVVSGADDRLLTAEQHFQNFK 60
           MERLTVIA  F ILLS TVAYG SSDQINGA +DD+LIRQVVSGADDRLLTAEQHFQNFK
Sbjct: 1   MERLTVIALSFAILLSATVAYGESSDQINGAEDDDILIRQVVSGADDRLLTAEQHFQNFK 60

Query: 61  LKFGKTYATDEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGVN 120
           LKFGKTYATDEEHDYRFR+FKANLR+AKRHQKLDPGAVHGVT+FSDLTE EFRKNFVGVN
Sbjct: 61  LKFGKTYATDEEHDYRFRIFKANLRKAKRHQKLDPGAVHGVTQFSDLTEDEFRKNFVGVN 120

Query: 121 RLRLPADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLST 180
           RLRLPADA +APILPTD+LASDFDWR+ GAVTPVK+QGSCGSCWSFSAVGALEGANFLST
Sbjct: 121 RLRLPADAQKAPILPTDNLASDFDWRDHGAVTPVKNQGSCGSCWSFSAVGALEGANFLST 180

Query: 181 GNLVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGTD 240
           G LVSLSEQQLVDCDHECDPEEAGACDAGC GGLMNSAFEYILK+GGLEREEDYPYTG D
Sbjct: 181 GQLVSLSEQQLVDCDHECDPEEAGACDAGCNGGLMNSAFEYILKAGGLEREEDYPYTGKD 240

Query: 241 RGPCKFQNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYIC 300
           RGPCKFQN KIAASV+NFSV+S DADQIAANL+KNGPLA+GINA FMQTY+ GVSCPYIC
Sbjct: 241 RGPCKFQNAKIAASVSNFSVISLDADQIAANLLKNGPLAIGINAAFMQTYMKGVSCPYIC 300

Query: 301 SKRNLDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVESM 360
           SKRNL+HGVL+VGYGSAG+APIRFKEKPYWIIKNSWGENWGENGYY ICKGKNICGVESM
Sbjct: 301 SKRNLNHGVLLVGYGSAGYAPIRFKEKPYWIIKNSWGENWGENGYYLICKGKNICGVESM 360

Query: 361 VSSVAATKN 370
           VSSVAAT+N
Sbjct: 361 VSSVAATQN 369

BLAST of Spg033738 vs. ExPASy TrEMBL
Match: A0A6J1KL64 (cysteine protease RD19A-like OS=Cucurbita maxima OX=3661 GN=LOC111496246 PE=3 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 4.1e-196
Identity = 331/369 (89.70%), Postives = 350/369 (94.85%), Query Frame = 0

Query: 1   MERLTVIAFFFIILLSTTVAYGVSSDQINGANEDDLLIRQVVSGADDRLLTAEQHFQNFK 60
           MERLTVIA  F ILLS TVAYGVSSDQING  +DDLLIRQVVSG DDRLLTAEQHFQNFK
Sbjct: 1   MERLTVIALSFAILLSATVAYGVSSDQINGVEDDDLLIRQVVSGDDDRLLTAEQHFQNFK 60

Query: 61  LKFGKTYATDEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGVN 120
           LKFGKTYATDEEHDYRFR+FKANLR+AKRHQKLDPGA+HGVT+FSDLTE EFRKNFVGVN
Sbjct: 61  LKFGKTYATDEEHDYRFRIFKANLRKAKRHQKLDPGAIHGVTQFSDLTEDEFRKNFVGVN 120

Query: 121 RLRLPADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLST 180
           RLRLPADA +APILPTD+LASDFDWR+ GAVTPVK+QGSCGSCWSFSAVGALEGANFLST
Sbjct: 121 RLRLPADAQKAPILPTDNLASDFDWRDHGAVTPVKNQGSCGSCWSFSAVGALEGANFLST 180

Query: 181 GNLVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGTD 240
           G LVSLSEQQLVDCDHECDPEEAGACDAGC GGLM SAFEYILK+GGLEREEDYPYTG D
Sbjct: 181 GKLVSLSEQQLVDCDHECDPEEAGACDAGCNGGLMTSAFEYILKAGGLEREEDYPYTGKD 240

Query: 241 RGPCKFQNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYIC 300
           RGPCKFQN KIAASV+NFSV+S DADQIAANL+KNGPLA+GINA FMQTY+ GVSCPYIC
Sbjct: 241 RGPCKFQNAKIAASVSNFSVISLDADQIAANLLKNGPLAIGINAAFMQTYMKGVSCPYIC 300

Query: 301 SKRNLDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVESM 360
           SKRNL+HGVL+VGYGSAG+APIRFKEKPYWIIKNSWGENWGENGYY ICKGKNICGVESM
Sbjct: 301 SKRNLNHGVLLVGYGSAGYAPIRFKEKPYWIIKNSWGENWGENGYYLICKGKNICGVESM 360

Query: 361 VSSVAATKN 370
           VSSVAAT+N
Sbjct: 361 VSSVAATQN 369

BLAST of Spg033738 vs. ExPASy TrEMBL
Match: A0A0A0LPD9 (Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_2G363570 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.6e-192
Identity = 326/368 (88.59%), Postives = 345/368 (93.75%), Query Frame = 0

Query: 1   MERLTVIAFFFIILLSTTVAYGVSSDQINGA--NEDDLLIRQVVSGADDRLLTAEQHFQN 60
           MER   I  FF ILLS TVAYGVSSDQIN A  +E+D+LIRQVVSGADDR LTAEQHFQ+
Sbjct: 1   MERFNAIPLFFAILLSATVAYGVSSDQINSAVSDEEDILIRQVVSGADDRPLTAEQHFQD 60

Query: 61  FKLKFGKTYATDEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVG 120
           FKLKFGKTY TDEEHDYRFRVFKANLR+AKRHQKLDP AVHGVTRFSDLTESEFR+NFVG
Sbjct: 61  FKLKFGKTYTTDEEHDYRFRVFKANLRKAKRHQKLDPDAVHGVTRFSDLTESEFRENFVG 120

Query: 121 VNRLRLPADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFL 180
           +NRLRLPADAH+APILPTD+LASDFDWR+ GAVTPVKDQGSCGSCWSFSAVGALEGANFL
Sbjct: 121 LNRLRLPADAHQAPILPTDNLASDFDWRDQGAVTPVKDQGSCGSCWSFSAVGALEGANFL 180

Query: 181 STGNLVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTG 240
           STG L+SLSEQQLVDCDHECDPEEAGACDAGC GGLM SAFEYI+K+GGLEREEDYPYTG
Sbjct: 181 STGKLISLSEQQLVDCDHECDPEEAGACDAGCNGGLMTSAFEYIVKAGGLEREEDYPYTG 240

Query: 241 TDRGPCKFQNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPY 300
           TDRG CKFQNGKIAAS ANFSV+S DADQIAANLVKNGPLA+GINAVFMQTY+ G+SCPY
Sbjct: 241 TDRGSCKFQNGKIAASAANFSVISNDADQIAANLVKNGPLAIGINAVFMQTYMKGISCPY 300

Query: 301 ICSKRNLDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVE 360
           ICSKRNLDHGVL+VGYG+AGFAPIR KEKPYWIIKNSWGENWGENGYYFICKGKNICG E
Sbjct: 301 ICSKRNLDHGVLLVGYGAAGFAPIRLKEKPYWIIKNSWGENWGENGYYFICKGKNICGSE 360

Query: 361 SMVSSVAA 367
           SMVSSVAA
Sbjct: 361 SMVSSVAA 368

BLAST of Spg033738 vs. ExPASy TrEMBL
Match: A0A1S3BB40 (cysteine proteinase RD19a-like OS=Cucumis melo OX=3656 GN=LOC103488015 PE=3 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 1.4e-191
Identity = 327/368 (88.86%), Postives = 344/368 (93.48%), Query Frame = 0

Query: 1   MERLTVIAFFFIILLSTTVAYGVSSDQING--ANEDDLLIRQVVSGADDRLLTAEQHFQN 60
           M R   IA FF ILLS TVAYGV SDQI+G  ++ +DLLIRQVVSGADDRLLTAEQ FQN
Sbjct: 1   MARFNAIALFFAILLSATVAYGVFSDQIDGDVSDGEDLLIRQVVSGADDRLLTAEQRFQN 60

Query: 61  FKLKFGKTYATDEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVG 120
           FKLKFGKTYAT EEHDYRFRVFK NLR+AKRHQKLDP AVHGVTRFSDLTESEFR+NFVG
Sbjct: 61  FKLKFGKTYATAEEHDYRFRVFKDNLRKAKRHQKLDPDAVHGVTRFSDLTESEFRENFVG 120

Query: 121 VNRLRLPADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFL 180
           +NRLRLPADAH+APILPTD+LASDFDWR+ GAVTPVKDQGSCGSCWSFSAVGALEGANFL
Sbjct: 121 LNRLRLPADAHQAPILPTDNLASDFDWRDKGAVTPVKDQGSCGSCWSFSAVGALEGANFL 180

Query: 181 STGNLVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTG 240
           STG L+SLSEQQLVDCDHECDPEEAGACDAGC GGLM SAFEYILK+GGLEREEDYPYTG
Sbjct: 181 STGKLISLSEQQLVDCDHECDPEEAGACDAGCNGGLMTSAFEYILKAGGLEREEDYPYTG 240

Query: 241 TDRGPCKFQNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPY 300
           TDRG CKFQNGKIAAS ANFSV+S DADQIAANLVKNGPLA+GINAVFMQTY+ GVSCPY
Sbjct: 241 TDRGSCKFQNGKIAASAANFSVISNDADQIAANLVKNGPLAIGINAVFMQTYMKGVSCPY 300

Query: 301 ICSKRNLDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVE 360
           ICSKRNLDHGVL+VGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKN+CG E
Sbjct: 301 ICSKRNLDHGVLLVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNVCGSE 360

Query: 361 SMVSSVAA 367
           SMVSSVAA
Sbjct: 361 SMVSSVAA 368

BLAST of Spg033738 vs. ExPASy TrEMBL
Match: A0A6J1CTJ1 (cysteine protease RD19A-like OS=Momordica charantia OX=3673 GN=LOC111014075 PE=3 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 3.0e-191
Identity = 324/369 (87.80%), Postives = 346/369 (93.77%), Query Frame = 0

Query: 1   MERLTVIAFFFIILLSTTVAYGVSSDQINGANEDDLLIRQVVSGADDRLLTAEQHFQNFK 60
           ME  TVIA FF ILLS TVAYGVSSDQI+G   DDLLIRQVVSGADDRLLTAEQHF+NFK
Sbjct: 1   MELPTVIALFFAILLSATVAYGVSSDQIDG---DDLLIRQVVSGADDRLLTAEQHFENFK 60

Query: 61  LKFGKTYATDEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGVN 120
           LKFGK+Y + EEHDYRFRVF+ANLRRA+RHQKLDPGAVHGVTRFSDLTE+EFR NFVGVN
Sbjct: 61  LKFGKSYGSSEEHDYRFRVFQANLRRARRHQKLDPGAVHGVTRFSDLTEAEFRSNFVGVN 120

Query: 121 RLRLPADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLST 180
           RLRLPADA +APILPT++L SDFDWR+LGAVTPVKDQGSCGSCWSFSAVGA+EGANFLST
Sbjct: 121 RLRLPADAQKAPILPTEELPSDFDWRDLGAVTPVKDQGSCGSCWSFSAVGAVEGANFLST 180

Query: 181 GNLVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGTD 240
           GNLVSLSEQQLVDCDHECDPEE GACDAGC GGLMNSAFEYILK+GGLEREEDYPYTGTD
Sbjct: 181 GNLVSLSEQQLVDCDHECDPEEYGACDAGCNGGLMNSAFEYILKAGGLEREEDYPYTGTD 240

Query: 241 RGPCKFQNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYIC 300
           RGPCKFQNGK+AASVANFSV+S+DA+QIAANLVKNGPLA+ INAVFMQTYIGGVSCPYIC
Sbjct: 241 RGPCKFQNGKVAASVANFSVISKDAEQIAANLVKNGPLAIAINAVFMQTYIGGVSCPYIC 300

Query: 301 SKRNLDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVESM 360
           SKRNL+HGVL+VGYGS  FAPIR KEKPYWIIKNSWGE WGENGYYFICKG N CG ES+
Sbjct: 301 SKRNLNHGVLLVGYGSGAFAPIRLKEKPYWIIKNSWGERWGENGYYFICKGHNTCGAESL 360

Query: 361 VSSVAATKN 370
           VSSVAAT+N
Sbjct: 361 VSSVAATQN 366

BLAST of Spg033738 vs. TAIR 10
Match: AT4G39090.1 (Papain family cysteine protease )

HSP 1 Score: 545.0 bits (1403), Expect = 4.5e-155
Identity = 265/368 (72.01%), Postives = 310/368 (84.24%), Query Frame = 0

Query: 1   MERLTVIAFFFIILLSTTVAYGVSSDQINGANEDDLLIRQVVSGADDRLLTAEQHFQNFK 60
           M+RL +  +F + +LS  +   VSS  +N  + DDL+IRQVV GA+ ++LT+E HF  FK
Sbjct: 1   MDRLKL--YFSVFVLSFFIV-SVSSSDVN--DGDDLVIRQVVGGAEPQVLTSEDHFSLFK 60

Query: 61  LKFGKTYATDEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGV- 120
            KFGK YA++EEHDYRF VFKANLRRA+RHQKLDP A HGVT+FSDLT SEFRK  +GV 
Sbjct: 61  RKFGKVYASNEEHDYRFSVFKANLRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHLGVR 120

Query: 121 NRLRLPADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLS 180
           +  +LP DA++APILPT++L  DFDWR+ GAVTPVK+QGSCGSCWSFSA GALEGANFL+
Sbjct: 121 SGFKLPKDANKAPILPTENLPEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGANFLA 180

Query: 181 TGNLVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGT 240
           TG LVSLSEQQLVDCDHECDPEEA +CD+GC GGLMNSAFEY LK+GGL +EEDYPYTG 
Sbjct: 181 TGKLVSLSEQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKTGGLMKEEDYPYTGK 240

Query: 241 DRGPCKFQNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYI 300
           D   CK    KI ASV+NFSV+S D +QIAANLVKNGPLAV INA +MQTYIGGVSCPYI
Sbjct: 241 DGKTCKLDKSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVSCPYI 300

Query: 301 CSKRNLDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVES 360
           C++R L+HGVL+VGYG+AG+AP RFKEKPYWIIKNSWGE WGENG+Y ICKG+NICGV+S
Sbjct: 301 CTRR-LNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICGVDS 360

Query: 361 MVSSVAAT 368
           MVS+VAAT
Sbjct: 361 MVSTVAAT 362

BLAST of Spg033738 vs. TAIR 10
Match: AT2G21430.1 (Papain family cysteine protease )

HSP 1 Score: 518.5 bits (1334), Expect = 4.5e-147
Identity = 248/356 (69.66%), Postives = 293/356 (82.30%), Query Frame = 0

Query: 13  ILLSTTVAYGVSSDQINGANEDDLLIRQVVSGADDRLLTAEQHFQNFKLKFGKTYATDEE 72
           +L S ++ +   S  + G  ++D+LIRQVV   + ++L++E HF  FK KFGK Y + EE
Sbjct: 7   VLFSVSLIFVFVSVSVCG--DEDVLIRQVVDETEPKVLSSEDHFTLFKKKFGKVYGSIEE 66

Query: 73  HDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGV-NRLRLPADAHEA 132
           H YRF VFKANL RA RHQK+DP A HGVT+FSDLT SEFR+  +GV    +LP DA++A
Sbjct: 67  HYYRFSVFKANLLRAMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGVKGGFKLPKDANQA 126

Query: 133 PILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLSTGNLVSLSEQQL 192
           PILPT +L  +FDWR+ GAVTPVK+QGSCGSCWSFS  GALEGA+FL+TG LVSLSEQQL
Sbjct: 127 PILPTQNLPEEFDWRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFLATGKLVSLSEQQL 186

Query: 193 VDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGTDRGPCKFQNGKI 252
           VDCDHECDPEE G+CD+GC GGLMNSAFEY LK+GGL RE+DYPYTGTD G CK    KI
Sbjct: 187 VDCDHECDPEEEGSCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTGTDGGSCKLDRSKI 246

Query: 253 AASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYICSKRNLDHGVLI 312
            ASV+NFSVVS + DQIAANL+KNGPLAV INA +MQTYIGGVSCPYICS+R L+HGVL+
Sbjct: 247 VASVSNFSVVSINEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPYICSRR-LNHGVLL 306

Query: 313 VGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVESMVSSVAAT 368
           VGYGSAGF+  R KEKPYWIIKNSWGE+WGENG+Y ICKG+NICGV+S+VS+VAAT
Sbjct: 307 VGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDSLVSTVAAT 359

BLAST of Spg033738 vs. TAIR 10
Match: AT4G16190.1 (Papain family cysteine protease )

HSP 1 Score: 501.9 bits (1291), Expect = 4.4e-142
Identity = 244/363 (67.22%), Postives = 292/363 (80.44%), Query Frame = 0

Query: 10  FFIILLSTTVAYGVSSDQINGANEDDLL--IRQVV-SGADDRLLTAEQHFQNFKLKFGKT 69
           FF ++ +T +A  + S  I+G   D  +  IRQVV    D++LL AE HF  FK K+ KT
Sbjct: 6   FFFLIAATLLAGSLGSTVISGEVTDGFVNPIRQVVPEENDEQLLNAEHHFTLFKSKYEKT 65

Query: 70  YATDEEHDYRFRVFKANLRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGVNR--LRL 129
           YAT  EHD+RFRVFKANLRRA+R+Q LDP AVHGVT+FSDLT  EFR+ F+G+ R   RL
Sbjct: 66  YATQVEHDHRFRVFKANLRRARRNQLLDPSAVHGVTQFSDLTPKEFRRKFLGLKRRGFRL 125

Query: 130 PADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLSTGNLV 189
           P D   APILPT DL ++FDWR  GAVTPVK+QG CGSCWSFSA+GALEGA+FL+T  LV
Sbjct: 126 PTDTQTAPILPTSDLPTEFDWREQGAVTPVKNQGMCGSCWSFSAIGALEGAHFLATKELV 185

Query: 190 SLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGTDRGPC 249
           SLSEQQLVDCDHECDP +A +CD+GC GGLMN+AFEY LK+GGL +EEDYPYTG D   C
Sbjct: 186 SLSEQQLVDCDHECDPAQANSCDSGCSGGLMNNAFEYALKAGGLMKEEDYPYTGRDHTAC 245

Query: 250 KFQNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYICSKRN 309
           KF   KI ASV+NFSVVS D DQIAANLV++GPLA+ INA++MQTYIGGVSCPY+CSK +
Sbjct: 246 KFDKSKIVASVSNFSVVSSDEDQIAANLVQHGPLAIAINAMWMQTYIGGVSCPYVCSK-S 305

Query: 310 LDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENGYYFICKG-KNICGVESMVSS 367
            DHGVL+VG+GS+G+APIR KEKPYWIIKNSWG  WGE+GYY IC+G  N+CG+++MVS+
Sbjct: 306 QDHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGYYKICRGPHNMCGMDTMVST 365

BLAST of Spg033738 vs. TAIR 10
Match: AT3G54940.2 (Papain family cysteine protease )

HSP 1 Score: 419.1 bits (1076), Expect = 3.7e-117
Identity = 201/345 (58.26%), Postives = 253/345 (73.33%), Query Frame = 0

Query: 31  ANEDDLLIRQVVSGADDRLLT-------AEQHFQNFKLKFGKTYATDEEHDYRFRVFKAN 90
           A+ +DL IRQV   AD+R +         E  F+ F   +GK Y+T EE+ +R  +F  N
Sbjct: 21  ASVEDLTIRQVT--ADNRRIRPNLLGTHTESKFRLFMSDYGKNYSTREEYIHRLGIFAKN 80

Query: 91  LRRAKRHQKLDPGAVHGVTRFSDLTESEFRKNFVGVNRL---RLPADAHEAPILPTDDLA 150
           + +A  HQ +DP AVHGVT+FSDLTE EF++ + GV  +   R      EAP++  D L 
Sbjct: 81  VLKAAEHQMMDPSAVHGVTQFSDLTEEEFKRMYTGVADVGGSRGGTVGAEAPMVEVDGLP 140

Query: 151 SDFDWRNLGAVTPVKDQGSCGSCWSFSAVGALEGANFLSTGNLVSLSEQQLVDCDHECDP 210
            DFDWR  G VT VK+QG+CGSCW+FS  GA EGA+F+STG L+SLSEQQLVDCD  CDP
Sbjct: 141 EDFDWREKGGVTEVKNQGACGSCWAFSTTGAAEGAHFVSTGKLLSLSEQQLVDCDQACDP 200

Query: 211 EEAGACDAGCGGGLMNSAFEYILKSGGLEREEDYPYTGTDRGPCKFQNGKIAASVANFSV 270
           ++  ACD GCGGGLM +A+EY++++GGLE E  YPYTG  RG CKF   K+A  V NF+ 
Sbjct: 201 KDKKACDNGCGGGLMTNAYEYLMEAGGLEEERSYPYTG-KRGHCKFDPEKVAVRVLNFTT 260

Query: 271 VSEDADQIAANLVKNGPLAVGINAVFMQTYIGGVSCPYICSKRNLDHGVLIVGYGSAGFA 330
           +  D +QIAANLV++GPLAVG+NAVFMQTYIGGVSCP ICSKRN++HGVL+VGYGS GF+
Sbjct: 261 IPLDENQIAANLVRHGPLAVGLNAVFMQTYIGGVSCPLICSKRNVNHGVLLVGYGSKGFS 320

Query: 331 PIRFKEKPYWIIKNSWGENWGENGYYFICKGKNICGVESMVSSVA 366
            +R   KPYWIIKNSWG+ WGENGYY +C+G +ICG+ SMVS+VA
Sbjct: 321 ILRLSNKPYWIIKNSWGKKWGENGYYKLCRGHDICGINSMVSAVA 362

BLAST of Spg033738 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 221.9 bits (564), Expect = 8.7e-58
Identity = 128/322 (39.75%), Postives = 182/322 (56.52%), Query Frame = 0

Query: 52  AEQHFQNFKLKFGKTYATDEEHDYRFRVFKANLRRAKRHQKLDPGAVH--GVTRFSDLTE 111
           A + ++ + ++  K Y    E + RF +FK NL+  + H  + P   +  G+TRF+DLT 
Sbjct: 39  ARRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSI-PNRTYEVGLTRFADLTN 98

Query: 112 SEFRKNFV--GVNRLRLPADAHEAPILPTDDLASDFDWRNLGAVTPVKDQGSCGSCWSFS 171
            EFR  ++   + R R+P    +      D L    DWR  GAV PVKDQGSCGSCW+FS
Sbjct: 99  DEFRAIYLRSKMERTRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFS 158

Query: 172 AVGALEGANFLSTGNLVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFEYILKSGG 231
           A+GA+EG N + TG L+SLSEQ+LVDCD         + + GCGGGLM+ AF++I+++GG
Sbjct: 159 AIGAVEGINQIKTGELISLSEQELVDCD--------TSYNDGCGGGLMDYAFKFIIENGG 218

Query: 232 LEREEDYPYTGTDRGPCKF-QNGKIAASVANFSVVSEDADQIAANLVKNGPLAVGINA-- 291
           ++ EEDYPY  TD   C   +      ++  +  V ++ ++     + N P++V I A  
Sbjct: 219 IDTEEDYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGG 278

Query: 292 VFMQTYIGGVSCPYICSKRNLDHGVLIVGYGSAGFAPIRFKEKPYWIIKNSWGENWGENG 351
              Q Y  GV     C   +LDHGV+ VGYGS G        + YWI++NSWG NWGE+G
Sbjct: 279 RAFQLYTSGVFTG-TCG-TSLDHGVVAVGYGSEG-------GQDYWIVRNSWGSNWGESG 338

Query: 352 YY----FICKGKNICGVESMVS 363
           Y+     I +    CGV  M S
Sbjct: 339 YFKLERNIKESSGKCGVAMMAS 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022951324.17.6e-19790.24cysteine protease RD19A-like [Cucurbita moschata] >XP_022951325.1 cysteine prote... [more]
XP_023002391.18.4e-19689.70cysteine protease RD19A-like [Cucurbita maxima][more]
XP_023537303.11.1e-19590.71cysteine protease RD19A-like [Cucurbita pepo subsp. pepo][more]
XP_038884968.11.4e-19589.49cysteine protease RD19A-like [Benincasa hispida][more]
KAG6585563.13.2e-19590.44Cysteine protease RD19A, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
P432966.3e-15472.01Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1[more]
P258044.9e-14667.40Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1[more]
P432956.4e-14669.66Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 S... [more]
Q9SUL16.2e-14167.22Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 S... [more]
Q107169.5e-13464.21Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GIG13.7e-19790.24cysteine protease RD19A-like OS=Cucurbita moschata OX=3662 GN=LOC111454190 PE=3 ... [more]
A0A6J1KL644.1e-19689.70cysteine protease RD19A-like OS=Cucurbita maxima OX=3661 GN=LOC111496246 PE=3 SV... [more]
A0A0A0LPD91.6e-19288.59Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_2G363570 PE=3 SV=1[more]
A0A1S3BB401.4e-19188.86cysteine proteinase RD19a-like OS=Cucumis melo OX=3656 GN=LOC103488015 PE=3 SV=1[more]
A0A6J1CTJ13.0e-19187.80cysteine protease RD19A-like OS=Momordica charantia OX=3673 GN=LOC111014075 PE=3... [more]
Match NameE-valueIdentityDescription
AT4G39090.14.5e-15572.01Papain family cysteine protease [more]
AT2G21430.14.5e-14769.66Papain family cysteine protease [more]
AT4G16190.14.4e-14267.22Papain family cysteine protease [more]
AT3G54940.23.7e-11758.26Papain family cysteine protease [more]
AT3G19390.18.7e-5839.75Granulin repeat cysteine protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 307..317
score: 58.7
coord: 157..172
score: 64.3
coord: 329..335
score: 75.14
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 139..365
e-value: 4.3E-103
score: 358.5
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 140..363
e-value: 4.7E-72
score: 242.6
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 56..112
e-value: 2.5E-22
score: 90.2
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 56..112
e-value: 2.7E-11
score: 43.7
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 30..365
e-value: 1.5E-96
score: 325.9
NoneNo IPR availablePANTHERPTHR12411:SF783CYSTEINE PROTEASE RD19C-RELATEDcoord: 32..365
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 32..365
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 157..168
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 329..348
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 305..315
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 143..364
e-value: 1.59253E-94
score: 279.124
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 53..364

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg033738.1Spg033738.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0005764 lysosome
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008234 cysteine-type peptidase activity