Spg026182 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg026182
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold7: 1528307 .. 1531746 (+)
RNA-Seq Expression	Spg026182
Synteny	Spg026182

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAAAATTGCGAAATGAGTTTGCAACAAAGAGAGAGCGATGGGGAGAGACAAAACAAAATGCATCAAGGGCGGTGTTGAATTTGGTATGCTGGTTTTAGAGTAAAGCAAAGAAACTTCAAAAATTTTCTCTTCTACTTTACGTGTAGGCATATTGGGAAATGGCGACCTCCCCTTAATCTCCTTTCAAGCAAAGCCATATGCCCACTAAAATGGCTTCTTTATTTTCCTTTATAAACTCTAACCTCTTTCACTGGATCATTGCATTAACACAAATTACTCTAGTAATCTCTTCTACCAAGTCGACTGAAATGGATAAATTCTTCCTGCTTTCCCTTCTTGGTATGCTGCTCATTGCCTATGGAGTTTCAGAGAGTGTCGTGCCTGACAGCGACATTGACATGGTTAGAGAACTTGAAAGTTTCAACATTGAAGAAGAGGACGACGATATAGTGGAGACATTTGAGGTCCCGGTGTGGAGAAGCGAGCGCGGGAGCAAGGTTCTGATGAATGTGGATAGTTTCGACGCTGTTGGAGATGGAGTTACAGATGATACAATGGTACATAGACATTTCTGTTTGGAGATATGGAATTTGCAGAGACAATACTGAATGAAAGTGTTTGTTTTTCGATCAGGCTTTCAGGAAGGCATGGGAGACTGCCTGCAGCACTTCAAAATCAGTTCTTTTGGTGCCAAAAGAAGGGAGATATCTGGTGAATGCAACCACTTTTAAAGGGCCATGCAAAGATGGTATGGTGATCCAGGTAAGCCACAGGATTATAAGGATTTCATTTCCCCAAATAAACAAGGTAGAAGATCATGGTTGAAAGATAGAGTTAACTTTGCAGATTGAAGGAACAATAGTTGCACCAGATGAACCAGAGAAATGGGATCCAAAATTTCCACGACAATGGCTGGAGTTTACCAAACTAAATGCAGCTACCTTTCAAGGGGATGGAGTTATCGACGGCTCTGGCGAGAAATGGTGGGCAGAATCCTGTAAAAAGAACAAGTCCAGGGTAAATTTTTGAACTGTCTCTGTGGTTGTTTCTTAGGCTATGTTGTTGAAATGTTAATCATTTCTGGTTTTCTGGTTGTTCTTTTTCCAGCCCTGCAAAGGAGCACCAACAGTAAGTAAAAAACTACTCTCCTCTCAATGAAAACCATGTTTTTTCCCTGTGTAACACATTCTGATTTGAGGGCTTTCATGCAGGCATTAACCATTGATTCAAGTTCAAATATAAGAGTAAAAGGCCTTACTATTCAGAACAGCCAACAGATGCATTTCATCATTGCTCGGTCGGAAACTGTTCGGATTACGGAGGTAAGAGTCTCAGCTCCAGGAGATAGCCCCAATACCGATGGGATCCATATCACTCAATCAACTAATGTTGTAGTTCAGAACTGCAAGATTTCAACAGGTTTTCATTCTGACCTTCATCATGTTATCATTGATACTACCATTTTACCATATAAACTGGCTGAAAAGGGTATTGATTAATAATGGTTCTGCAATTTATATGCAGGCGATGACTGCATCTCTATTGTCAATGCTAGTTCTGGCATCAAGATGAAAGGAATATCATGTGGACCAGGACACGGGATCAGGTATCGGCAAATGTTTTCATTTCACTCGGCATTATTCGATTTATTTCAACCTCCAAAACAAGATCAACGTCTTAGAATTCCTTGCTCTTTGTTTGTTTGTTATGAAACTGCGTTCCCTAGCATTGGAAGTTTAGGGAAAGACAACTCCACTGGCATTGTGACAAAGGTGGTGTTAGACACAGCCTTCCTCAGAGAGACAACAAATGGTGTTAGAATCAAGACTTGGCAGGTTAGATCACAGCTTTAACCACCATTTTGGTTGATTACAACCAAGCACACGGGTAAATTACTCTATTTATGTGGTATAGGGAGGTTCTGGATATGTTCGTGCCGTACGATTCGAAAATGTGAGAATGGAAGATGTTGAGAATCCCATTATCATTGATCAATTCTACTGTGACTCTCCTACTACTTGTGAAACTCAGGTATTATCTTCAAAATCCAGAATTCAGCTTCTAATCAAGCAATAACATTAAAAATAAGATCATAATTAGACAATAAGACAAAGTTCGTCCATATCAATAATGAAGTTAAATTCTTTTGTTTTTGTTAGACATCTGCCGTGAAAATAAGCCAAATCATGTATCGAAACATAAGCGGAACCACAACAAGCAAGAACGCAATGAAATTTGCCTGTAGCGACAGCGTTCCATGCAGCAACATAATTCTAAGCAATGTCAATTTGGAGAAGACGGATGGAACAGTGGAAACCTATTGTCACGCTGCTCAAGGCTTTGGATATGGCGTCGTGCACCCATCGGCAGACTGCCTAACCTCCAGCGACAATGACTTTGCAGCTTCAAACCAGACTCAAGTTCCGGAGCCTAGGGCAGTGCAACTCCCTACTGAGCAGCACATTGTTCACACTGAGCTCTGACCTTGTTGTCCTGTCTATCTATACTAAACTACTAAGGCGATCATATTTTGGTAGAGCTTGGTTTTCAGGTGACCAACTCAGGAAATATTTGTAGCATATATTTACATAGCACACAATTATACCTACCCTTTTGAAATCTAAATAGAAATATAGACTACTAAGTAAATGATTGTACTATTGCTAGAAGAAAATATACATATTTTGAGTTTATAGTAAAGTTTTTTGATACTAAAGTTCATATCAAATGGTTGATGGCCCTCTAACTATTTATAATATACATATTACTCCAAAAAAGAAAAAGGAACTATATAATAATAAACACAAATGTGAAGATAGGGACTAGTGGACAATAATGAGAAACAAAAGGGGAAGATTGAGGAAGAAGGACCGCTGTTAATGGAGTATAGTGGACGCCCAGCAAACAGATCAAACTCAAGCCTTCCTTTCAACATTCTTAAAGGGAATGCCAAAAAGAAAGTAAAAACACAGCAGTTTCTGGTAGAATACCAACTTCTTGAAAGCGAAAGATGGCGTACAGTAGAAGTCATGGAACAAGACAAAGTGGTCCAATGGAGTAATTGAACCAAAATGGCACTTCCACTAACTGATGGTCAATGAAACAGAGGAAACGCACCCCCACAACCCATAAAACTAAATTCCCTTTTGAAGAAACAGAAAATGAAAATAAAGAACTAATGGCATCCAATGCTCTAACTTGAAGGGCAGTATGACCTTATAAGCTATGGAGAAAGGCCAAGGCATACCCGATACCCTTATTGGCAGAGTGCTAAACTTGAAGGGCAGTGACAATGACAGTGAGCTTAGAAGCTATGAAGAAGAGCCCCAAAGGCATACCCTTATCGGCAAAGAGTCCTAAACTTGAAGGGTATTGACCTTTGCTACATTTCAAACAGAGAGAGGAAGCAAAAGATTAGTGGGGGTGGCTCAGAAACAGA

mRNA sequence

ATGCCCACTAAAATGGCTTCTTTATTTTCCTTTATAAACTCTAACCTCTTTCACTGGATCATTGCATTAACACAAATTACTCTAGTAATCTCTTCTACCAAGTCGACTGAAATGGATAAATTCTTCCTGCTTTCCCTTCTTGGTATGCTGCTCATTGCCTATGGAGTTTCAGAGAGTGTCGTGCCTGACAGCGACATTGACATGGTTAGAGAACTTGAAAGTTTCAACATTGAAGAAGAGGACGACGATATAGTGGAGACATTTGAGGTCCCGGTGTGGAGAAGCGAGCGCGGGAGCAAGGTTCTGATGAATGTGGATAGTTTCGACGCTGTTGGAGATGGAGTTACAGATGATACAATGGCTTTCAGGAAGGCATGGGAGACTGCCTGCAGCACTTCAAAATCAGTTCTTTTGGTGCCAAAAGAAGGGAGATATCTGGTGAATGCAACCACTTTTAAAGGGCCATGCAAAGATGGTATGGTGATCCAGATTGAAGGAACAATAGTTGCACCAGATGAACCAGAGAAATGGGATCCAAAATTTCCACGACAATGGCTGGAGTTTACCAAACTAAATGCAGCTACCTTTCAAGGGGATGGAGTTATCGACGGCTCTGGCGAGAAATGGTGGGCAGAATCCTGTAAAAAGAACAAGTCCAGGCCCTGCAAAGGAGCACCAACAGCATTAACCATTGATTCAAGTTCAAATATAAGAGTAAAAGGCCTTACTATTCAGAACAGCCAACAGATGCATTTCATCATTGCTCGGTCGGAAACTGTTCGGATTACGGAGGTAAGAGTCTCAGCTCCAGGAGATAGCCCCAATACCGATGGGATCCATATCACTCAATCAACTAATGTTGTAGTTCAGAACTGCAAGATTTCAACAGGCGATGACTGCATCTCTATTGTCAATGCTAGTTCTGGCATCAAGATGAAAGGAATATCATGTGGACCAGGACACGGGATCAGGTATCGGCAAATGTTTTCATTTCACTCGGCATTATTCGATTTATTTCAACCTCCAAAACAAGATCAACGTCTTAGAATTCCTTGCTCTTTGTTTGTTTGTTATGAAACTGCGTTCCCTAGCATTGGAAGTTTAGGGAAAGACAACTCCACTGGCATTGTGACAAAGGTGGTGTTAGACACAGCCTTCCTCAGAGAGACAACAAATGGTGTTAGAATCAAGACTTGGCAGGGAGGTTCTGGATATGTTCGTGCCGTACGATTCGAAAATGTGAGAATGGAAGATGTTGAGAATCCCATTATCATTGATCAATTCTACTGTGACTCTCCTACTACTTGTGAAACTCAGACATCTGCCGTGAAAATAAGCCAAATCATGTATCGAAACATAAGCGGAACCACAACAAGCAAGAACGCAATGAAATTTGCCTGTAGCGACAGCGTTCCATGCAGCAACATAATTCTAAGCAATGTCAATTTGGAGAAGACGGATGGAACAGTGGAAACCTATTGTCACGCTGCTCAAGGCTTTGGATATGGCGTCGTGCACCCATCGGCAGACTGCCTAACCTCCAGCGACAATGACTTTGCAGCTTCAAACCAGACTCAAGTTCCGGAGCCTAGGGCAGTGCAACTCCCTACTGAGCAGCACATTGTTCACACTGAGCTCTGA

Coding sequence (CDS)

ATGCCCACTAAAATGGCTTCTTTATTTTCCTTTATAAACTCTAACCTCTTTCACTGGATCATTGCATTAACACAAATTACTCTAGTAATCTCTTCTACCAAGTCGACTGAAATGGATAAATTCTTCCTGCTTTCCCTTCTTGGTATGCTGCTCATTGCCTATGGAGTTTCAGAGAGTGTCGTGCCTGACAGCGACATTGACATGGTTAGAGAACTTGAAAGTTTCAACATTGAAGAAGAGGACGACGATATAGTGGAGACATTTGAGGTCCCGGTGTGGAGAAGCGAGCGCGGGAGCAAGGTTCTGATGAATGTGGATAGTTTCGACGCTGTTGGAGATGGAGTTACAGATGATACAATGGCTTTCAGGAAGGCATGGGAGACTGCCTGCAGCACTTCAAAATCAGTTCTTTTGGTGCCAAAAGAAGGGAGATATCTGGTGAATGCAACCACTTTTAAAGGGCCATGCAAAGATGGTATGGTGATCCAGATTGAAGGAACAATAGTTGCACCAGATGAACCAGAGAAATGGGATCCAAAATTTCCACGACAATGGCTGGAGTTTACCAAACTAAATGCAGCTACCTTTCAAGGGGATGGAGTTATCGACGGCTCTGGCGAGAAATGGTGGGCAGAATCCTGTAAAAAGAACAAGTCCAGGCCCTGCAAAGGAGCACCAACAGCATTAACCATTGATTCAAGTTCAAATATAAGAGTAAAAGGCCTTACTATTCAGAACAGCCAACAGATGCATTTCATCATTGCTCGGTCGGAAACTGTTCGGATTACGGAGGTAAGAGTCTCAGCTCCAGGAGATAGCCCCAATACCGATGGGATCCATATCACTCAATCAACTAATGTTGTAGTTCAGAACTGCAAGATTTCAACAGGCGATGACTGCATCTCTATTGTCAATGCTAGTTCTGGCATCAAGATGAAAGGAATATCATGTGGACCAGGACACGGGATCAGGTATCGGCAAATGTTTTCATTTCACTCGGCATTATTCGATTTATTTCAACCTCCAAAACAAGATCAACGTCTTAGAATTCCTTGCTCTTTGTTTGTTTGTTATGAAACTGCGTTCCCTAGCATTGGAAGTTTAGGGAAAGACAACTCCACTGGCATTGTGACAAAGGTGGTGTTAGACACAGCCTTCCTCAGAGAGACAACAAATGGTGTTAGAATCAAGACTTGGCAGGGAGGTTCTGGATATGTTCGTGCCGTACGATTCGAAAATGTGAGAATGGAAGATGTTGAGAATCCCATTATCATTGATCAATTCTACTGTGACTCTCCTACTACTTGTGAAACTCAGACATCTGCCGTGAAAATAAGCCAAATCATGTATCGAAACATAAGCGGAACCACAACAAGCAAGAACGCAATGAAATTTGCCTGTAGCGACAGCGTTCCATGCAGCAACATAATTCTAAGCAATGTCAATTTGGAGAAGACGGATGGAACAGTGGAAACCTATTGTCACGCTGCTCAAGGCTTTGGATATGGCGTCGTGCACCCATCGGCAGACTGCCTAACCTCCAGCGACAATGACTTTGCAGCTTCAAACCAGACTCAAGTTCCGGAGCCTAGGGCAGTGCAACTCCCTACTGAGCAGCACATTGTTCACACTGAGCTCTGA

Protein sequence

MPTKMASLFSFINSNLFHWIIALTQITLVISSTKSTEMDKFFLLSLLGMLLIAYGVSESVVPDSDIDMVRELESFNIEEEDDDIVETFEVPVWRSERGSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEGRYLVNATTFKGPCKDGMVIQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFSFHSALFDLFQPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNDFAASNQTQVPEPRAVQLPTEQHIVHTEL

Homology

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BLAST of Spg026182 vs. NCBI nr
Match: XP_008446196.1 (PREDICTED: probable polygalacturonase At1g80170 [Cucumis melo])

HSP 1 Score: 918.3 bits (2372), Expect = 3.1e-263
Identity = 470/546 (86.08%), Postives = 483/546 (88.46%), Query Frame = 0


Query: 1   MPTKMASLFSFINSNLFHWIIALTQITLVISSTKSTEMDKFFLLSLLGMLLIAYGVSESV 60
           MPTKMASLFSFINSNLF  IIAL QITLVISS  STEMDK FLLSLLG+L+IAYGVS + 
Sbjct: 1   MPTKMASLFSFINSNLFLSIIALIQITLVISSNNSTEMDKLFLLSLLGLLIIAYGVSGTF 60

Query: 61  VPDSDIDMVRELESFNIEEEDDDIVETFEVPVWRSERGSKVLMNVDSFDAVGDGVTDDTM 120
           V DSDIDM+ ELESF+IEEEDDD+V ++ VP WRSERGSK+LMNVDSFDAVGDGVTDDTM
Sbjct: 61  VSDSDIDMIEELESFDIEEEDDDVVYSYTVPAWRSERGSKILMNVDSFDAVGDGVTDDTM 120

Query: 121 AFRKAWETACSTSKSVLLVPKEGRYLVNATTFKGPCKDGMVIQIEGTIVAPDEPEKWDPK 180
           AFRKAWETACSTSKSVLLVPKE RYLVNATTFKGPCKDGMVIQIEGTIVAPDEPE WDPK
Sbjct: 121 AFRKAWETACSTSKSVLLVPKEKRYLVNATTFKGPCKDGMVIQIEGTIVAPDEPELWDPK 180

Query: 181 FPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRPCKGAPTALTIDSSSNIRVK 240
           FPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRPCKGAPTA TIDSSSNIRVK
Sbjct: 181 FPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRPCKGAPTAFTIDSSSNIRVK 240

Query: 241 GLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNCKISTGDDC 300
           GLTIQNSQQMHF IARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQN KISTGDDC
Sbjct: 241 GLTIQNSQQMHFTIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNSKISTGDDC 300

Query: 301 ISIVNASSGIKMKGISCGPGHGIRYRQMFSFHSALFDLFQPPKQDQRLRIPCSLFVCYET 360
           ISIVNASSGIKMKGISCGPGHGI                                     
Sbjct: 301 ISIVNASSGIKMKGISCGPGHGI------------------------------------- 360

Query: 361 AFPSIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVE 420
              SIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVR+VRFENVRMEDVE
Sbjct: 361 ---SIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVE 420

Query: 421 NPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSN 480
           NPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSN
Sbjct: 421 NPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSN 480

Query: 481 VNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNDFAASNQTQVPEPRAVQLPTEQH 540
           VNLEKTDGTVETYCHAAQGFGYG+VHPSADCLTSSDN+FAA +QTQV E   VQLPTEQH
Sbjct: 481 VNLEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAALDQTQVLELETVQLPTEQH 506

Query: 541 IVHTEL 547
           IVHTEL
Sbjct: 541 IVHTEL 506

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BLAST of Spg026182 vs. NCBI nr
Match: XP_011655619.1 (probable polygalacturonase At1g80170 isoform X1 [Cucumis sativus])

HSP 1 Score: 860.5 bits (2222), Expect = 7.7e-246
Identity = 436/509 (85.66%), Postives = 451/509 (88.61%), Query Frame = 0


Query: 38  MDKFFLLSLLGMLLIAYGVSESVVPDSDIDMVRELESFNIEEEDDDIVETFEVPVWRSER 97
           MDK FLLSLLG+ +IAYGVS + V DSDIDM++ELESF+IEEEDDD V ++ VPVWRSER
Sbjct: 1   MDKLFLLSLLGLFIIAYGVSGAFVSDSDIDMIKELESFDIEEEDDDDVYSYTVPVWRSER 60

Query: 98  GSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEGRYLVNATTFKGPCK 157
           GSK+LMNVDSFDA+GDGVTDDTMAFRKAWETACSTSKSVLLVPKE  YLVNATTFKGPCK
Sbjct: 61  GSKILMNVDSFDAIGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKTYLVNATTFKGPCK 120

Query: 158 DGMVIQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 217
           DGMVIQIEGTIVAPDEPE WDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN
Sbjct: 121 DGMVIQIEGTIVAPDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180

Query: 218 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTD 277
           KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHF IARSETVRIT VRVSAPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITGVRVSAPGDSPNTD 240

Query: 278 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFSFHSALFD 337
           GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGI              
Sbjct: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGI-------------- 300

Query: 338 LFQPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIK 397
                                     SIGSLGKDNSTGIVTKVVLDTA+LRETTNGVRIK
Sbjct: 301 --------------------------SIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIK 360

Query: 398 TWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTT 457
           TWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCE+QTSAVKISQIMYRNISGTT
Sbjct: 361 TWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTT 420

Query: 458 TSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDN 517
           TSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDN
Sbjct: 421 TSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDN 469

Query: 518 DFAASNQTQVPEPRAVQLPTEQHIVHTEL 547
           +FA+S+QTQV E   VQL TEQHIVHTEL
Sbjct: 481 NFASSDQTQVLELETVQLSTEQHIVHTEL 469

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BLAST of Spg026182 vs. NCBI nr
Match: XP_022966897.1 (probable polygalacturonase At1g80170 [Cucurbita maxima])

HSP 1 Score: 860.5 bits (2222), Expect = 7.7e-246
Identity = 436/530 (82.26%), Postives = 462/530 (87.17%), Query Frame = 0


Query: 20  IIALTQITLVISSTKSTEMDKFF---LLSLLGMLLIAYGVSESVVPDSDIDMVRELESFN 79
           +I LTQIT VISSTKST+MDKFF   LL LL +L+IAYGVS S+ P+SDIDMV+EL++FN
Sbjct: 1   MIPLTQITSVISSTKSTKMDKFFLLPLLPLLSLLIIAYGVSCSIDPESDIDMVKELKTFN 60

Query: 80  IEEEDDDIVETFEVPVWRSERGSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSV 139
           IEE+DD++V+T+ +PVW+SERGS+ LMNVDSFDAVGDGVTDDTMAFRKAWETACS +KSV
Sbjct: 61  IEEQDDNVVDTYAIPVWKSERGSRSLMNVDSFDAVGDGVTDDTMAFRKAWETACSAAKSV 120

Query: 140 LLVPKEGRYLVNATTFKGPCKDGMVIQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATF 199
           LLVPKE RYLVNATTFKGPC DGM+IQIEGTI+APDEPEKWDPK PRQWLEFTKLNAATF
Sbjct: 121 LLVPKEKRYLVNATTFKGPCNDGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATF 180

Query: 200 QGDGVIDGSGEKWWAESCKKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIAR 259
           QGDGVIDGSGEKWWAESCKKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHF IAR
Sbjct: 181 QGDGVIDGSGEKWWAESCKKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIAR 240

Query: 260 SETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGIS 319
           SETVRITEVRVS+PGDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGIS
Sbjct: 241 SETVRITEVRVSSPGDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGIS 300

Query: 320 CGPGHGIRYRQMFSFHSALFDLFQPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGI 379
           CGPGHGI                                        SIGSLGKDNS GI
Sbjct: 301 CGPGHGI----------------------------------------SIGSLGKDNSAGI 360

Query: 380 VTKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTC 439
           V+KVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRM+DVENPIIIDQFYCDSP  C
Sbjct: 361 VSKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNAC 420

Query: 440 ETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHA 499
           ETQTSA+K+SQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHA
Sbjct: 421 ETQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHA 480

Query: 500 AQGFGYGVVHPSADCLTSSDNDFAASNQTQVPEPRAVQLPTEQHIVHTEL 547
           AQGFGYGVVHPSADCLTSSDNDFAA +QT        QLPTEQHIVHTEL
Sbjct: 481 AQGFGYGVVHPSADCLTSSDNDFAAKDQT--------QLPTEQHIVHTEL 482

[Close]

BLAST of Spg026182 vs. NCBI nr
Match: XP_022956901.1 (probable polygalacturonase At1g80170 [Cucurbita moschata])

HSP 1 Score: 845.5 bits (2183), Expect = 2.6e-241
Identity = 427/509 (83.89%), Postives = 446/509 (87.62%), Query Frame = 0


Query: 38  MDKFFLLSLLGMLLIAYGVSESVVPDSDIDMVRELESFNIEEEDDDIVETFEVPVWRSER 97
           MDKFFLLSLLGML+I YGVS S+VPD DIDMV+ELESF+IEEEDD  V+T+ VP WRSE 
Sbjct: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGDIDMVKELESFDIEEEDDSAVDTYAVPAWRSEH 60

Query: 98  GSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEGRYLVNATTFKGPCK 157
           GSK+L+NVDSFDAVGDGVTDDTMAFRKAWETACS+S SVLLVPKEGRYLVNAT FKGPCK
Sbjct: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120

Query: 158 DGMVIQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 217
            GMVIQIEGTIVAPDEPE WDPK PRQWLEFTKL AATFQGDG+IDGSGEKWWAESCKKN
Sbjct: 121 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLTAATFQGDGIIDGSGEKWWAESCKKN 180

Query: 218 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTD 277
           KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARS+TVR++EVRVSAPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240

Query: 278 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFSFHSALFD 337
           GIHITQSTNVVV+NCKISTGDDCISIVNASSGIKMK ISCGPGHGI              
Sbjct: 241 GIHITQSTNVVVENCKISTGDDCISIVNASSGIKMKRISCGPGHGI-------------- 300

Query: 338 LFQPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIK 397
                                     SIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIK
Sbjct: 301 --------------------------SIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIK 360

Query: 398 TWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTT 457
           TWQGGSGYVRAVRFENVRME+VENPIIIDQFYCD  TTCE Q SAVKISQIMYRNISGTT
Sbjct: 361 TWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDH-TTCEPQASAVKISQIMYRNISGTT 420

Query: 458 TSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDN 517
           TSKNAMKFACSDS PCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCL+SSDN
Sbjct: 421 TSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLSSSDN 468

Query: 518 DFAASNQTQVPEPRAVQLPTEQHIVHTEL 547
           D AAS++TQVPE  AVQLPTE+HIVHTEL
Sbjct: 481 DIAASDETQVPELEAVQLPTERHIVHTEL 468

[Close]

BLAST of Spg026182 vs. NCBI nr
Match: KAG6573734.1 (putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 843.6 bits (2178), Expect = 9.7e-241
Identity = 421/509 (82.71%), Postives = 446/509 (87.62%), Query Frame = 0


Query: 38  MDKFFLLSLLGMLLIAYGVSESVVPDSDIDMVRELESFNIEEEDDDIVETFEVPVWRSER 97
           MDKFFLL LL +L+IAYGVS S+ P+SDIDMV+ELE+F+IEE+DD++V+T+ +PVW+SER
Sbjct: 1   MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER 60

Query: 98  GSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEGRYLVNATTFKGPCK 157
           GS+ LMNVDSFDAVGDG+TDDTMAFRKAWETACS +KSVLLVPKE RYLVNATTFKGPC 
Sbjct: 61  GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKRYLVNATTFKGPCN 120

Query: 158 DGMVIQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 217
           DGM+IQIEGTI+APDEPEKWDPK PRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN
Sbjct: 121 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180

Query: 218 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTD 277
           KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHF IARSETVRITEVRVS+PGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 240

Query: 278 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFSFHSALFD 337
           GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGI              
Sbjct: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGI-------------- 300

Query: 338 LFQPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIK 397
                                     SIGSLGKDNS GIV+KVVLDTAFLRETTNGVRIK
Sbjct: 301 --------------------------SIGSLGKDNSAGIVSKVVLDTAFLRETTNGVRIK 360

Query: 398 TWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTT 457
           TWQGGSGYVRAVRFENVRM+DVENPIIIDQFYCDSP  CETQTSA+K+SQIMYRNISGTT
Sbjct: 361 TWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACETQTSAIKVSQIMYRNISGTT 420

Query: 458 TSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDN 517
           TSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDN
Sbjct: 421 TSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDN 461

Query: 518 DFAASNQTQVPEPRAVQLPTEQHIVHTEL 547
           DFAA +QT        QLPTEQHIVHTEL
Sbjct: 481 DFAAKDQT--------QLPTEQHIVHTEL 461

[Close]

BLAST of Spg026182 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.3e-99
Identity = 193/429 (44.99%), Postives = 256/429 (59.67%), Query Frame = 0


Query: 94  RSERGSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTS-KSVLLVPKEGRYLVNATTF 153
           RSER    L++V +F A G+GVTDDT AF  AW+TACS+  K+ +LVP+    L+     
Sbjct: 48  RSER----LLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDL 107

Query: 154 KGPCKDGMVIQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAE 213
            GPCK  + +QI GTI+AP++P+ W+    R+WL F  L+  T +G G ++G G++WW  
Sbjct: 108 SGPCKARLTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWER 167

Query: 214 SCKKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGD 273
           SCK N S PC+GAPTALT     N+RV+ L + +SQQMH  +     V I+ ++V AP  
Sbjct: 168 SCKHNHSNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPAT 227

Query: 274 SPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFSFH 333
           SPNTDGIHI+ S  +V+ N  +STGDDCISIV  S+ I +  I CGPGHGI         
Sbjct: 228 SPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGI--------- 287

Query: 334 SALFDLFQPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRETTN 393
                                          SIGSLGK  S   V  + +DTA + +T N
Sbjct: 288 -------------------------------SIGSLGKSKSWEEVRDITVDTAIISDTAN 347

Query: 394 GVRIKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRN 453
           GVRIKTWQGGSG V  + F N++M +V NPIIIDQ+YCDS   C  QTSA+ I  I + +
Sbjct: 348 GVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVH 407

Query: 454 ISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKT--DGTVETYCHAAQGFGYGVVHPSAD 513
           + GT+ SK A+K +CSDS PC NI+L +++LE +  DG  E++C  A G   G V+P   
Sbjct: 408 VRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEAYGSSSGQVYP-PP 430

Query: 514 CLTSSDNDF 520
           CL S D  F
Sbjct: 468 CL-SDDTSF 430

[Close]

BLAST of Spg026182 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 6.6e-91
Identity = 185/407 (45.45%), Postives = 240/407 (58.97%), Query Frame = 0


Query: 103 MNVDSFDAVGDGVTDDTMAFRKAWETAC-STSKSVLLVPKEGRYLVNATTFKGPCKDGMV 162
           +NVD F A GDG  DDT AF KAW+ AC STS +VLLVPK+  YLV   +F GPCK G+ 
Sbjct: 90  VNVDDFGAKGDG-RDDTKAFEKAWKAACSSTSSAVLLVPKK-NYLVRPISFSGPCKSGLT 149

Query: 163 IQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRP 222
           +QI GTI A D+   +  K  R WL F  +     +G G I+G+G+ WW  SCK NK+ P
Sbjct: 150 MQIYGTIEASDDRSDY-RKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKALP 209

Query: 223 CKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTDGIHI 282
           CK APTALT   S ++ VK L I+N+QQ+H        V+ + + V+AP +SPNTDGIH+
Sbjct: 210 CKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPNTDGIHV 269

Query: 283 TQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFSFHSALFDLFQP 342
           T + N+ + +C I TGDDCISIVN S  +++  I+CGPGHGI                  
Sbjct: 270 TGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGI------------------ 329

Query: 343 PKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQG 402
                                 SIGSLG  NS   V+ VV++ A L  TTNGVRIKTWQG
Sbjct: 330 ----------------------SIGSLGYGNSEAHVSDVVVNGAKLCGTTNGVRIKTWQG 389

Query: 403 GSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKN 462
           GSG    ++F+NV M +VENPIIIDQ YCD    C+ Q+SAV++  ++Y+NI GT  S  
Sbjct: 390 GSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKPCQEQSSAVQVKNVVYQNIKGTCASNV 449

Query: 463 AMKFACSDSVPCSNIILSNVNLEKTDG-TVETYCHAAQGFGYGVVHP 508
           A+ F CS   PC  I+L +V+LE   G   +  C+  +    GVV P
Sbjct: 450 AITFDCSKRFPCQGIVLEDVDLEIEGGAAAKALCNNVELSETGVVSP 453

[Close]

BLAST of Spg026182 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 3.6e-89
Identity = 178/402 (44.28%), Postives = 231/402 (57.46%), Query Frame = 0


Query: 91  PVWRSERGSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEGRYLVNAT 150
           PV   E      ++VD F A GDG TDDT AF KAW+ ACS S SVL+VP+   YL+   
Sbjct: 73  PVGGPETSPDTDISVDDFGARGDG-TDDTKAFEKAWKDACS-SGSVLIVPENKNYLLKQI 132

Query: 151 TFKGPCKDGMVIQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWW 210
           TF GPCK  + ++I GTI A  +   W     ++W+EF  ++  T +G G  +G+GE WW
Sbjct: 133 TFSGPCKSDLRVKIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWW 192

Query: 211 AESCKKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAP 270
             SCK+ KS PCK APTALT  S  N+ V  L+I++SQ+MH    + + V  + + V+AP
Sbjct: 193 DSSCKRKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAP 252

Query: 271 GDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFS 330
             SPNTDGIHIT +  + V N  I TGDDCISI + S  +    I+CGPGHGI       
Sbjct: 253 EHSPNTDGIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGI------- 312

Query: 331 FHSALFDLFQPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRET 390
                                            SIGSLG  NS   V+ V++D   L +T
Sbjct: 313 ---------------------------------SIGSLGDRNSEAHVSGVLVDGGNLFDT 372

Query: 391 TNGVRIKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMY 450
           TNG+RIKTWQGGSG  + ++F+N+ M +V NPIIIDQ+YCDS   C  Q SAVK+S + Y
Sbjct: 373 TNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCDSKDPCPEQESAVKVSNVAY 432

Query: 451 RNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVET 493
            NI GT+ S+ A+KF CS S PC   I+ N+NL    G   T
Sbjct: 433 MNIRGTSASEVAVKFDCSKSSPCQGYIVGNINLVGNGGKETT 432

[Close]

BLAST of Spg026182 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 5.8e-87
Identity = 171/416 (41.11%), Postives = 232/416 (55.77%), Query Frame = 0


Query: 101 VLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEGRYLVNATTFKGPCKDGM 160
           ++ NV+ + AVGDG  D T AF K W  AC+   +V LVP   +++VN   F GPC+   
Sbjct: 58  IVFNVEHYGAVGDGKHDSTDAFEKTWNAACNKLSAVFLVPANKKFVVNNLVFYGPCQPHF 117

Query: 161 VIQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSR 220
             +++GTI A  +P KW  K  + W+ F +L      G GVIDG G +WW++ CK    R
Sbjct: 118 SFKVDGTIAAYPDPAKW--KNSKIWMHFARLTDFNLMGTGVIDGQGNRWWSDQCKTINGR 177

Query: 221 PC---KGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTD 280
                KG PTA+ ID S ++ VK LT+ NS + H +    + V+I  +++ AP DSPNTD
Sbjct: 178 TVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGVKIQGIKIKAPRDSPNTD 237

Query: 281 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFSFHSALFD 340
           GI I  S    ++ C I TGDDC+++   SS I +K ++CGPGHG+              
Sbjct: 238 GIDIFASKRFEIEKCTIGTGDDCVAVGTGSSNITIKDLTCGPGHGM-------------- 297

Query: 341 LFQPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIK 400
                                     SIGSLGK NS   V+ V LD A   +T NG+RIK
Sbjct: 298 --------------------------SIGSLGKGNSRSEVSFVHLDGAKFIDTQNGLRIK 357

Query: 401 TWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTT 460
           TWQGGSG    + +ENV M + ENPI+I+QFYC S   C+ Q SAVKI  + ++NI GT+
Sbjct: 358 TWQGGSGLASHITYENVEMINAENPILINQFYCTSAAACKNQRSAVKIQDVTFKNIHGTS 417

Query: 461 TSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHA-AQGFGYGVVHPSADCL 513
            +  A++  CSDSVPCSNI LSNV L+ T G V T  +  A G+    ++PS   L
Sbjct: 418 ATTAAIQLMCSDSVPCSNIKLSNVFLKLTSGKVATCVNKNANGYYTNPLNPSCKSL 431

[Close]

BLAST of Spg026182 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 3.2e-85
Identity = 171/406 (42.12%), Postives = 237/406 (58.37%), Query Frame = 0


Query: 103 MNVDSFDAVGDGVTDDTMAFRKAWETACSTS-KSVLLVPKEGRYLVNATTFKGPCKDGMV 162
           ++V +F A GDG TDDT AF+KAW+ ACST+  +  LVPK   YL+ +T F+GPCK    
Sbjct: 68  VSVSNFGAKGDGKTDDTQAFKKAWKKACSTNGVTTFLVPKGKTYLLKSTRFRGPCKSLRN 127

Query: 163 IQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQG--DGVIDGSGEKWWAESCKKNKS 222
            QI GT+ A    ++ D K    WL    +N  +  G   G+I+G+G+ WW  SCK +KS
Sbjct: 128 FQILGTLSA--STKRSDYKDKNHWLILEDVNNLSIDGGSTGIINGNGKTWWQNSCKIDKS 187

Query: 223 RPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTDGI 282
           +PC  APTALT+ +  N+ VK L ++N+QQ+   I +   V ++ V ++APGDSPNTDGI
Sbjct: 188 KPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNVEITAPGDSPNTDGI 247

Query: 283 HITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFSFHSALFDLF 342
           HIT + N+ V N  I TGDDCISI + +  +++  ++CGPGHGI                
Sbjct: 248 HITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGI---------------- 307

Query: 343 QPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTW 402
                                   SIGSLG DNS   V+ + +D A   E+ NGVRIKT+
Sbjct: 308 ------------------------SIGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTY 367

Query: 403 QGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTS 462
           QGGSG  + ++F+N+RME+V+NPIIIDQ YCD    CE Q SAV++  ++Y+NISGT+ +
Sbjct: 368 QGGSGTAKNIKFQNIRMENVKNPIIIDQDYCDK-DKCEDQESAVQVKNVVYKNISGTSAT 427

Query: 463 KNAMKFACSDSVPCSNIILSNVNLE-----------KTDGTVETYC 495
             A+   CS+  PC  I+L NV ++           K  GTV   C
Sbjct: 428 DVAITLNCSEKYPCQGIVLENVKIKGGTASCKNANVKNQGTVSPKC 430

[Close]

BLAST of Spg026182 vs. ExPASy TrEMBL
Match: A0A1S3BF58 (probable polygalacturonase At1g80170 OS=Cucumis melo OX=3656 GN=LOC103489000 PE=3 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 1.5e-263
Identity = 470/546 (86.08%), Postives = 483/546 (88.46%), Query Frame = 0


Query: 1   MPTKMASLFSFINSNLFHWIIALTQITLVISSTKSTEMDKFFLLSLLGMLLIAYGVSESV 60
           MPTKMASLFSFINSNLF  IIAL QITLVISS  STEMDK FLLSLLG+L+IAYGVS + 
Sbjct: 1   MPTKMASLFSFINSNLFLSIIALIQITLVISSNNSTEMDKLFLLSLLGLLIIAYGVSGTF 60

Query: 61  VPDSDIDMVRELESFNIEEEDDDIVETFEVPVWRSERGSKVLMNVDSFDAVGDGVTDDTM 120
           V DSDIDM+ ELESF+IEEEDDD+V ++ VP WRSERGSK+LMNVDSFDAVGDGVTDDTM
Sbjct: 61  VSDSDIDMIEELESFDIEEEDDDVVYSYTVPAWRSERGSKILMNVDSFDAVGDGVTDDTM 120

Query: 121 AFRKAWETACSTSKSVLLVPKEGRYLVNATTFKGPCKDGMVIQIEGTIVAPDEPEKWDPK 180
           AFRKAWETACSTSKSVLLVPKE RYLVNATTFKGPCKDGMVIQIEGTIVAPDEPE WDPK
Sbjct: 121 AFRKAWETACSTSKSVLLVPKEKRYLVNATTFKGPCKDGMVIQIEGTIVAPDEPELWDPK 180

Query: 181 FPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRPCKGAPTALTIDSSSNIRVK 240
           FPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRPCKGAPTA TIDSSSNIRVK
Sbjct: 181 FPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRPCKGAPTAFTIDSSSNIRVK 240

Query: 241 GLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNCKISTGDDC 300
           GLTIQNSQQMHF IARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQN KISTGDDC
Sbjct: 241 GLTIQNSQQMHFTIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNSKISTGDDC 300

Query: 301 ISIVNASSGIKMKGISCGPGHGIRYRQMFSFHSALFDLFQPPKQDQRLRIPCSLFVCYET 360
           ISIVNASSGIKMKGISCGPGHGI                                     
Sbjct: 301 ISIVNASSGIKMKGISCGPGHGI------------------------------------- 360

Query: 361 AFPSIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVE 420
              SIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVR+VRFENVRMEDVE
Sbjct: 361 ---SIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVE 420

Query: 421 NPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSN 480
           NPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSN
Sbjct: 421 NPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSN 480

Query: 481 VNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNDFAASNQTQVPEPRAVQLPTEQH 540
           VNLEKTDGTVETYCHAAQGFGYG+VHPSADCLTSSDN+FAA +QTQV E   VQLPTEQH
Sbjct: 481 VNLEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAALDQTQVLELETVQLPTEQH 506

Query: 541 IVHTEL 547
           IVHTEL
Sbjct: 541 IVHTEL 506

[Close]

BLAST of Spg026182 vs. ExPASy TrEMBL
Match: A0A6J1HP82 (probable polygalacturonase At1g80170 OS=Cucurbita maxima OX=3661 GN=LOC111466464 PE=3 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 3.7e-246
Identity = 436/530 (82.26%), Postives = 462/530 (87.17%), Query Frame = 0


Query: 20  IIALTQITLVISSTKSTEMDKFF---LLSLLGMLLIAYGVSESVVPDSDIDMVRELESFN 79
           +I LTQIT VISSTKST+MDKFF   LL LL +L+IAYGVS S+ P+SDIDMV+EL++FN
Sbjct: 1   MIPLTQITSVISSTKSTKMDKFFLLPLLPLLSLLIIAYGVSCSIDPESDIDMVKELKTFN 60

Query: 80  IEEEDDDIVETFEVPVWRSERGSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSV 139
           IEE+DD++V+T+ +PVW+SERGS+ LMNVDSFDAVGDGVTDDTMAFRKAWETACS +KSV
Sbjct: 61  IEEQDDNVVDTYAIPVWKSERGSRSLMNVDSFDAVGDGVTDDTMAFRKAWETACSAAKSV 120

Query: 140 LLVPKEGRYLVNATTFKGPCKDGMVIQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATF 199
           LLVPKE RYLVNATTFKGPC DGM+IQIEGTI+APDEPEKWDPK PRQWLEFTKLNAATF
Sbjct: 121 LLVPKEKRYLVNATTFKGPCNDGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATF 180

Query: 200 QGDGVIDGSGEKWWAESCKKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIAR 259
           QGDGVIDGSGEKWWAESCKKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHF IAR
Sbjct: 181 QGDGVIDGSGEKWWAESCKKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIAR 240

Query: 260 SETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGIS 319
           SETVRITEVRVS+PGDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGIS
Sbjct: 241 SETVRITEVRVSSPGDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGIS 300

Query: 320 CGPGHGIRYRQMFSFHSALFDLFQPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGI 379
           CGPGHGI                                        SIGSLGKDNS GI
Sbjct: 301 CGPGHGI----------------------------------------SIGSLGKDNSAGI 360

Query: 380 VTKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTC 439
           V+KVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRM+DVENPIIIDQFYCDSP  C
Sbjct: 361 VSKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNAC 420

Query: 440 ETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHA 499
           ETQTSA+K+SQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHA
Sbjct: 421 ETQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHA 480

Query: 500 AQGFGYGVVHPSADCLTSSDNDFAASNQTQVPEPRAVQLPTEQHIVHTEL 547
           AQGFGYGVVHPSADCLTSSDNDFAA +QT        QLPTEQHIVHTEL
Sbjct: 481 AQGFGYGVVHPSADCLTSSDNDFAAKDQT--------QLPTEQHIVHTEL 482

[Close]

BLAST of Spg026182 vs. ExPASy TrEMBL
Match: A0A0A0KQ65 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G600960 PE=3 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 3.7e-246
Identity = 436/509 (85.66%), Postives = 451/509 (88.61%), Query Frame = 0


Query: 38  MDKFFLLSLLGMLLIAYGVSESVVPDSDIDMVRELESFNIEEEDDDIVETFEVPVWRSER 97
           MDK FLLSLLG+ +IAYGVS + V DSDIDM++ELESF+IEEEDDD V ++ VPVWRSER
Sbjct: 1   MDKLFLLSLLGLFIIAYGVSGAFVSDSDIDMIKELESFDIEEEDDDDVYSYTVPVWRSER 60

Query: 98  GSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEGRYLVNATTFKGPCK 157
           GSK+LMNVDSFDA+GDGVTDDTMAFRKAWETACSTSKSVLLVPKE  YLVNATTFKGPCK
Sbjct: 61  GSKILMNVDSFDAIGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKTYLVNATTFKGPCK 120

Query: 158 DGMVIQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 217
           DGMVIQIEGTIVAPDEPE WDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN
Sbjct: 121 DGMVIQIEGTIVAPDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180

Query: 218 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTD 277
           KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHF IARSETVRIT VRVSAPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITGVRVSAPGDSPNTD 240

Query: 278 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFSFHSALFD 337
           GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGI              
Sbjct: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGI-------------- 300

Query: 338 LFQPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIK 397
                                     SIGSLGKDNSTGIVTKVVLDTA+LRETTNGVRIK
Sbjct: 301 --------------------------SIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIK 360

Query: 398 TWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTT 457
           TWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCE+QTSAVKISQIMYRNISGTT
Sbjct: 361 TWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTT 420

Query: 458 TSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDN 517
           TSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDN
Sbjct: 421 TSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDN 469

Query: 518 DFAASNQTQVPEPRAVQLPTEQHIVHTEL 547
           +FA+S+QTQV E   VQL TEQHIVHTEL
Sbjct: 481 NFASSDQTQVLELETVQLSTEQHIVHTEL 469

[Close]

BLAST of Spg026182 vs. ExPASy TrEMBL
Match: A0A6J1GXR9 (probable polygalacturonase At1g80170 OS=Cucurbita moschata OX=3662 GN=LOC111458447 PE=3 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 1.2e-241
Identity = 427/509 (83.89%), Postives = 446/509 (87.62%), Query Frame = 0


Query: 38  MDKFFLLSLLGMLLIAYGVSESVVPDSDIDMVRELESFNIEEEDDDIVETFEVPVWRSER 97
           MDKFFLLSLLGML+I YGVS S+VPD DIDMV+ELESF+IEEEDD  V+T+ VP WRSE 
Sbjct: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGDIDMVKELESFDIEEEDDSAVDTYAVPAWRSEH 60

Query: 98  GSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEGRYLVNATTFKGPCK 157
           GSK+L+NVDSFDAVGDGVTDDTMAFRKAWETACS+S SVLLVPKEGRYLVNAT FKGPCK
Sbjct: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120

Query: 158 DGMVIQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 217
            GMVIQIEGTIVAPDEPE WDPK PRQWLEFTKL AATFQGDG+IDGSGEKWWAESCKKN
Sbjct: 121 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLTAATFQGDGIIDGSGEKWWAESCKKN 180

Query: 218 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTD 277
           KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARS+TVR++EVRVSAPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240

Query: 278 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFSFHSALFD 337
           GIHITQSTNVVV+NCKISTGDDCISIVNASSGIKMK ISCGPGHGI              
Sbjct: 241 GIHITQSTNVVVENCKISTGDDCISIVNASSGIKMKRISCGPGHGI-------------- 300

Query: 338 LFQPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIK 397
                                     SIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIK
Sbjct: 301 --------------------------SIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIK 360

Query: 398 TWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTT 457
           TWQGGSGYVRAVRFENVRME+VENPIIIDQFYCD  TTCE Q SAVKISQIMYRNISGTT
Sbjct: 361 TWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDH-TTCEPQASAVKISQIMYRNISGTT 420

Query: 458 TSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDN 517
           TSKNAMKFACSDS PCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCL+SSDN
Sbjct: 421 TSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLSSSDN 468

Query: 518 DFAASNQTQVPEPRAVQLPTEQHIVHTEL 547
           D AAS++TQVPE  AVQLPTE+HIVHTEL
Sbjct: 481 DIAASDETQVPELEAVQLPTERHIVHTEL 468

[Close]

BLAST of Spg026182 vs. ExPASy TrEMBL
Match: A0A6J1IQQ8 (probable polygalacturonase At1g80170 OS=Cucurbita maxima OX=3661 GN=LOC111477357 PE=3 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 6.2e-241
Identity = 426/509 (83.69%), Postives = 447/509 (87.82%), Query Frame = 0


Query: 38  MDKFFLLSLLGMLLIAYGVSESVVPDSDIDMVRELESFNIEEEDDDIVETFEVPVWRSER 97
           MDKFFLLSLLGML+I YGVS S+VPD DIDMV+ELESF+IEEEDD  V+T+ VPVWRSE 
Sbjct: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGDIDMVKELESFDIEEEDDSAVDTYAVPVWRSEH 60

Query: 98  GSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEGRYLVNATTFKGPCK 157
           GSK+L+NVDSFDAVGDGVTDDTMAFRKAWETACS+S SVLLVPKEGRYLVNAT FKGPCK
Sbjct: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120

Query: 158 DGMVIQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 217
            GMVIQIEGTIVAP EPE WDPK PRQWLEFTKL+AATFQGDG+IDGSGEKWWAESCKKN
Sbjct: 121 HGMVIQIEGTIVAPGEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN 180

Query: 218 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTD 277
           KSRPCKGAPTALTI+SSSNIRVKGLTIQNSQQMHFIIARS+TVR++EVRVSAPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240

Query: 278 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFSFHSALFD 337
           GIHITQSTNVVV+NCKISTGDDCISIVNASSGIKMK ISCGPGHGI              
Sbjct: 241 GIHITQSTNVVVENCKISTGDDCISIVNASSGIKMKRISCGPGHGI-------------- 300

Query: 338 LFQPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIK 397
                                     SIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIK
Sbjct: 301 --------------------------SIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIK 360

Query: 398 TWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTT 457
           TWQGGSGYVRAVRFENVRME+VENPIIIDQFYCD  TTCE Q SAVKISQIMYRNISGTT
Sbjct: 361 TWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDH-TTCEPQASAVKISQIMYRNISGTT 420

Query: 458 TSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDN 517
           TSKNAMKFACSDS PCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCL+SSDN
Sbjct: 421 TSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLSSSDN 468

Query: 518 DFAASNQTQVPEPRAVQLPTEQHIVHTEL 547
           D AAS++TQVPE  AVQLPTE+HIVHTEL
Sbjct: 481 DIAASDETQVPELEAVQLPTERHIVHTEL 468

[Close]

BLAST of Spg026182 vs. TAIR 10
Match: AT1G23460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 619.0 bits (1595), Expect = 3.6e-177
Identity = 309/481 (64.24%), Postives = 368/481 (76.51%), Query Frame = 0


Query: 38  MDKFFLLSLLGMLLI-AYGVSESVVPDSDIDMVRELESFNIEEEDDDIVETFEVPVWRSE 97
           MDK F+LSLLG+LL+ AYG +  +V  +D+D++ ELE+F++  +DDD  +  + P + S 
Sbjct: 2   MDKLFILSLLGLLLVTAYGAAGKMV-YTDLDILDELENFDVLVDDDDDTKLLDWPSFTSR 61

Query: 98  RGSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEGRYLVNATTFKGPC 157
              K L+NVD+F A GDGV+DDT AF  AW  ACSTSKSV LVP+  RYLVNAT F GPC
Sbjct: 62  HSGKNLVNVDTFGAAGDGVSDDTQAFVSAWSKACSTSKSVFLVPEGRRYLVNATKFNGPC 121

Query: 158 KDGMVIQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKK 217
           +  ++IQI+GTIVAPDEP  WD KF R WLEF+KL    FQG GVIDGSG KWWA SCKK
Sbjct: 122 EQKLIIQIDGTIVAPDEPSNWDSKFQRIWLEFSKLKGVVFQGKGVIDGSGSKWWAASCKK 181

Query: 218 NKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNT 277
           NKS PCK APTALTI+SSS ++V GLTIQNSQQM+FIIARS++VR+++V VS+PGDSPNT
Sbjct: 182 NKSNPCKSAPTALTIESSSGVKVSGLTIQNSQQMNFIIARSDSVRVSKVMVSSPGDSPNT 241

Query: 278 DGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFSFHSALF 337
           DGIHIT STNV++Q+CKI TGDDC+SIVNASS IKMK I CGPGHGI             
Sbjct: 242 DGIHITGSTNVILQDCKIGTGDDCVSIVNASSNIKMKNIYCGPGHGI------------- 301

Query: 338 DLFQPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRI 397
                                      SIGSLGKDN+TGIVT+VVLDTA LRETTNG+RI
Sbjct: 302 ---------------------------SIGSLGKDNTTGIVTQVVLDTALLRETTNGLRI 361

Query: 398 KTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGT 457
           KT+QGGSGYV+ +RF NV M+DV NPI+IDQFYCDSPTTC+ QTSAVKISQIMYRNI+GT
Sbjct: 362 KTYQGGSGYVQGIRFTNVEMQDVANPILIDQFYCDSPTTCQNQTSAVKISQIMYRNITGT 421

Query: 458 TTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSD 517
           T S  A+KFACSD+VPCS+I+L+NVNLE  DG VE YC++A+GFGYGV+HPSADCL S D
Sbjct: 422 TKSAKAIKFACSDTVPCSHIVLNNVNLEGNDGQVEAYCNSAEGFGYGVIHPSADCLYSHD 441

[Close]

BLAST of Spg026182 vs. TAIR 10
Match: AT1G70500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 577.4 bits (1487), Expect = 1.2e-164
Identity = 297/511 (58.12%), Postives = 368/511 (72.02%), Query Frame = 0


Query: 38  MDKFFLLSLLGMLLIA-YGVSESVVPDSDIDMVRELESFNIEEED-DDIVETFEVPVWRS 97
           M K F++SL  +L++A YG +  ++   ++D++ +LE+ ++ E+D +D V  F+   + S
Sbjct: 1   MIKPFIISLFALLMVASYGATGKML-FKELDILGKLENLDVPEDDIEDDVTFFDFSSFTS 60

Query: 98  ERGSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEGRYLVNATTFKGP 157
           +   K L+NVDSF+A GDGV+DDT AF +AW  ACS   SVLLVP+   YLVNAT F GP
Sbjct: 61  QYSGKNLVNVDSFNASGDGVSDDTQAFIRAWTMACSAPNSVLLVPQGRSYLVNATKFDGP 120

Query: 158 CKDGMVIQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCK 217
           C++ ++IQI+GTI+APDEP +WDPKFPR WL+F+KL    FQG+GVIDGSG KWWA SCK
Sbjct: 121 CQEKLIIQIDGTIIAPDEPSQWDPKFPRNWLQFSKLQGVVFQGNGVIDGSGTKWWAASCK 180

Query: 218 KNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPN 277
           KNKS PC GAPTALTI SSSN+ V+GLTI+NSQQMH II RS TVRI+ V V++PGDSPN
Sbjct: 181 KNKSNPCVGAPTALTIYSSSNVYVRGLTIRNSQQMHLIIQRSTTVRISRVMVTSPGDSPN 240

Query: 278 TDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFSFHSAL 337
           TDGIHIT ST+VVVQ+ KISTGDDC+SIVN S+ IKMK I CGPGHGI            
Sbjct: 241 TDGIHITASTDVVVQDSKISTGDDCVSIVNGSAKIKMKRIYCGPGHGI------------ 300

Query: 338 FDLFQPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRETTNGVR 397
                                       SIGSLG+ +S G VT VVL+TAFL+ TTNG+R
Sbjct: 301 ----------------------------SIGSLGQGHSKGTVTAVVLETAFLKNTTNGLR 360

Query: 398 IKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISG 457
           IKTWQGG+GYV+ VRFENV M+DV NPIIIDQFYCDSP+TC+ QTSAV IS+IMYRNI+G
Sbjct: 361 IKTWQGGNGYVKGVRFENVVMQDVANPIIIDQFYCDSPSTCQNQTSAVHISEIMYRNITG 420

Query: 458 TTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSS 517
           TT S  A+ F CSD+VPCS+I+L+N+NLE  DG VE YC++A+GFGYGVVHPSADCL S 
Sbjct: 421 TTKSSKAINFKCSDAVPCSHIVLNNINLEGNDGKVEAYCNSAEGFGYGVVHPSADCLYSH 468

Query: 518 DNDFAASNQTQVPEPRAVQLPTEQHIVHTEL 547
           D+   + NQT       + +  E    H EL
Sbjct: 481 DD--KSLNQTDQYLAETIMVEKETENAHDEL 468

[Close]

BLAST of Spg026182 vs. TAIR 10
Match: AT1G80170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 365.2 bits (936), Expect = 9.4e-101
Identity = 193/429 (44.99%), Postives = 256/429 (59.67%), Query Frame = 0


Query: 94  RSERGSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTS-KSVLLVPKEGRYLVNATTF 153
           RSER    L++V +F A G+GVTDDT AF  AW+TACS+  K+ +LVP+    L+     
Sbjct: 48  RSER----LLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDL 107

Query: 154 KGPCKDGMVIQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAE 213
            GPCK  + +QI GTI+AP++P+ W+    R+WL F  L+  T +G G ++G G++WW  
Sbjct: 108 SGPCKARLTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWER 167

Query: 214 SCKKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGD 273
           SCK N S PC+GAPTALT     N+RV+ L + +SQQMH  +     V I+ ++V AP  
Sbjct: 168 SCKHNHSNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPAT 227

Query: 274 SPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFSFH 333
           SPNTDGIHI+ S  +V+ N  +STGDDCISIV  S+ I +  I CGPGHGI         
Sbjct: 228 SPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGI--------- 287

Query: 334 SALFDLFQPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRETTN 393
                                          SIGSLGK  S   V  + +DTA + +T N
Sbjct: 288 -------------------------------SIGSLGKSKSWEEVRDITVDTAIISDTAN 347

Query: 394 GVRIKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRN 453
           GVRIKTWQGGSG V  + F N++M +V NPIIIDQ+YCDS   C  QTSA+ I  I + +
Sbjct: 348 GVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVH 407

Query: 454 ISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKT--DGTVETYCHAAQGFGYGVVHPSAD 513
           + GT+ SK A+K +CSDS PC NI+L +++LE +  DG  E++C  A G   G V+P   
Sbjct: 408 VRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEAYGSSSGQVYP-PP 430

Query: 514 CLTSSDNDF 520
           CL S D  F
Sbjct: 468 CL-SDDTSF 430

[Close]

BLAST of Spg026182 vs. TAIR 10
Match: AT3G57510.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 317.4 bits (812), Expect = 2.3e-86
Identity = 171/406 (42.12%), Postives = 237/406 (58.37%), Query Frame = 0


Query: 103 MNVDSFDAVGDGVTDDTMAFRKAWETACSTS-KSVLLVPKEGRYLVNATTFKGPCKDGMV 162
           ++V +F A GDG TDDT AF+KAW+ ACST+  +  LVPK   YL+ +T F+GPCK    
Sbjct: 68  VSVSNFGAKGDGKTDDTQAFKKAWKKACSTNGVTTFLVPKGKTYLLKSTRFRGPCKSLRN 127

Query: 163 IQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQG--DGVIDGSGEKWWAESCKKNKS 222
            QI GT+ A    ++ D K    WL    +N  +  G   G+I+G+G+ WW  SCK +KS
Sbjct: 128 FQILGTLSA--STKRSDYKDKNHWLILEDVNNLSIDGGSTGIINGNGKTWWQNSCKIDKS 187

Query: 223 RPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTDGI 282
           +PC  APTALT+ +  N+ VK L ++N+QQ+   I +   V ++ V ++APGDSPNTDGI
Sbjct: 188 KPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNVEITAPGDSPNTDGI 247

Query: 283 HITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFSFHSALFDLF 342
           HIT + N+ V N  I TGDDCISI + +  +++  ++CGPGHGI                
Sbjct: 248 HITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGI---------------- 307

Query: 343 QPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTW 402
                                   SIGSLG DNS   V+ + +D A   E+ NGVRIKT+
Sbjct: 308 ------------------------SIGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTY 367

Query: 403 QGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTS 462
           QGGSG  + ++F+N+RME+V+NPIIIDQ YCD    CE Q SAV++  ++Y+NISGT+ +
Sbjct: 368 QGGSGTAKNIKFQNIRMENVKNPIIIDQDYCDK-DKCEDQESAVQVKNVVYKNISGTSAT 427

Query: 463 KNAMKFACSDSVPCSNIILSNVNLE-----------KTDGTVETYC 495
             A+   CS+  PC  I+L NV ++           K  GTV   C
Sbjct: 428 DVAITLNCSEKYPCQGIVLENVKIKGGTASCKNANVKNQGTVSPKC 430

[Close]

BLAST of Spg026182 vs. TAIR 10
Match: AT2G41850.1 (polygalacturonase abscission zone A. thaliana )

HSP 1 Score: 316.6 bits (810), Expect = 3.9e-86
Identity = 175/420 (41.67%), Postives = 243/420 (57.86%), Query Frame = 0


Query: 94  RSERGSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSV-LLVPKEGRYLVNATTF 153
           R+   +   ++V  F A GDG TDDT AF  AW+ ACS++ +V LLVPK   YL+ +   
Sbjct: 59  RASLRTPTTVSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQL 118

Query: 154 KGPCKDGMVIQIEGTIVAPDEPEKWDPKFPRQWLEFTKLNAATFQG--DGVIDGSGEKWW 213
            GPC   + +QI GT+ A    ++ D K   +W+ F  +N  +  G   GV+DG+GE WW
Sbjct: 119 TGPCNSILTVQIFGTLSA--SQKRSDYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETWW 178

Query: 214 AESCKKNKSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAP 273
             SCK+NK++PC  APTALT  +S ++ VK L ++N+QQ+   I +   V+++ V V+AP
Sbjct: 179 QNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAP 238

Query: 274 GDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGIRYRQMFS 333
            DSPNTDGIHIT + N+ V    I TGDDCISI + S  +++  I+CGPGHGI       
Sbjct: 239 ADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGI------- 298

Query: 334 FHSALFDLFQPPKQDQRLRIPCSLFVCYETAFPSIGSLGKDNSTGIVTKVVLDTAFLRET 393
                                            SIGSLG DNS   V+ V +D A L  T
Sbjct: 299 ---------------------------------SIGSLGDDNSKAFVSGVTVDGAKLSGT 358

Query: 394 TNGVRIKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMY 453
            NGVRIKT+QGGSG    + F+N++M++V+NPIIIDQ YCD  + C T+ SAV++  ++Y
Sbjct: 359 DNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDK-SKCTTEKSAVQVKNVVY 418

Query: 454 RNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSAD 511
           R+ISGT+ S+NA+ F CS + PC  I+L  VN++    T    C  A     G V P  +
Sbjct: 419 RDISGTSASENAITFNCSKNYPCQGIVLDRVNIKGGKAT----CTNANVVDKGAVLPQCN 431

The following BLAST results are available for this feature:

BLAST of Spg026182 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008446196.1	3.1e-263	86.08	PREDICTED: probable polygalacturonase At1g80170 [Cucumis melo]	[more]
XP_011655619.1	7.7e-246	85.66	probable polygalacturonase At1g80170 isoform X1 [Cucumis sativus]	[more]
XP_022966897.1	7.7e-246	82.26	probable polygalacturonase At1g80170 [Cucurbita maxima]	[more]
XP_022956901.1	2.6e-241	83.89	probable polygalacturonase At1g80170 [Cucurbita moschata]	[more]
KAG6573734.1	9.7e-241	82.71	putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Spg026182 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AJ5	1.3e-99	44.99	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
P35336	6.6e-91	45.45	Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1	[more]
Q02096	3.6e-89	44.28	Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1	[more]
Q9FY19	5.8e-87	41.11	Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1	[more]
O23147	3.2e-85	42.12	Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1	[more]

BLAST of Spg026182 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BF58	1.5e-263	86.08	probable polygalacturonase At1g80170 OS=Cucumis melo OX=3656 GN=LOC103489000 PE=...	[more]
A0A6J1HP82	3.7e-246	82.26	probable polygalacturonase At1g80170 OS=Cucurbita maxima OX=3661 GN=LOC111466464...	[more]
A0A0A0KQ65	3.7e-246	85.66	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G600960 PE=3 SV=1	[more]
A0A6J1GXR9	1.2e-241	83.89	probable polygalacturonase At1g80170 OS=Cucurbita moschata OX=3662 GN=LOC1114584...	[more]
A0A6J1IQQ8	6.2e-241	83.69	probable polygalacturonase At1g80170 OS=Cucurbita maxima OX=3661 GN=LOC111477357...	[more]

BLAST of Spg026182 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G23460.1	3.6e-177	64.24	Pectin lyase-like superfamily protein	[more]
AT1G70500.1	1.2e-164	58.12	Pectin lyase-like superfamily protein	[more]
AT1G80170.1	9.4e-101	44.99	Pectin lyase-like superfamily protein	[more]
AT3G57510.1	2.3e-86	42.12	Pectin lyase-like superfamily protein	[more]
AT2G41850.1	3.9e-86	41.67	polygalacturonase abscission zone A. thaliana	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 473..494 e-value: 7500.0 score: 1.0 coord: 406..427 e-value: 5200.0 score: 2.1 coord: 284..305 e-value: 18.0 score: 14.2 coord: 257..283 e-value: 75.0 score: 12.2
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 132..323 e-value: 1.2E-50 score: 172.5 coord: 364..494 e-value: 4.3E-36 score: 124.6
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 92..545 e-value: 1.7E-116 score: 391.6
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 25..324
None	No IPR available	PANTHER	PTHR31375:SF205	BNAA08G20470D PROTEIN	coord: 25..324 coord: 364..524
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 364..524
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 108..507

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg026182.1	Spg026182.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008234	cysteine-type peptidase activity
molecular_function	GO:0004650	polygalacturonase activity