Spg021948 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg021948
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Phosphopyruvate hydratase
Location	scaffold2: 4046313 .. 4049488 (+)
RNA-Seq Expression	Spg021948
Synteny	Spg021948

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCATATAGTTATTTTTCCAGCCCCACCAACCCTCTGGTGTCAAAATCTCCACTAGCGTCGCTTCTCGTCGCCAAGTCCCAACAACCACGCTGCTCTGTTTTGAACGCCCTTCCCTTTTTCGGCGGACTTTCCCACTTTCTCTACTTTCTTCTTCTTCTCTGTATTATTATATATACTCTCCCCATTTCCATTTCTTCCTCTCTCACCAAACCCATTTCATTTCTCCCCATCAAAACTTCCATGGCTGCTGCTCTTCCTTCCCCACCCACCATTAACATGAACCTCCTCGACAAAACCCTCCTTCCTTCCAAACCTCAATCTTTCATTACTTCAACAACTTCTTCGAAGCCTCTTCTTCGCTCCCATGGTGTTCGCTGCTCTGTTGCTGTGGCGCCTTCTGCGTCGACCAAGGAGTCGAGGGAATTTAAGTTGAAGTCCCTGAAGGCCCGGCAGATCATTGACAGCAGAGGCAATCCGACGGTCGAGGTTGATTTGATTACTGATGATCTCTACCGATCCGCTGTCCCCAGTGGGGCCTCCACTGGGATCTATGAGGCTTTGGAGCTTCGAGATGGAGATAAAAGCGTTTATGGAGGTAAAGGCGTGCTTACTGCTGTTAAGAACATCAATGAAATCTTGGCCCCAAAGCTCGTCGGCGTCGATATCAGGTGGATTTAAGTTTGTCTTTGATGAACATCAATTTGAATGTTTATAATACTGAGTGCAAGTCGTGAAAAGTGAAATTGTGTTATTCTGATCGAATTGTTTGGTCTACTTTCTGACATAATACGAACATAATTCTCCTCTCCGAGTGTTTTACTGTTTTGGGTCTGCTACCTTTTCATCCTGATTGTGGGATTTGTGTAAAAGTTGGCTTAGTTCACTCTGAGATTTCATTTTCACCCTGATTGTTGCAAGACAAGGATGTGAATGGAACCGAAAAGATGGTGGAAATTTTCGTTGAACTTTCTAATATCTGACTGTGAAAGCTGGTAGTCTTTTCATTGAATTAAGTGCTGAAGATGTGAAATTCCAAGCTTTTTTGTTGTTCTGTCCAAGCTTATTTCACTTTTCACTTCTGCTTCCTGACCTAAGCTTGTCAACTTCTGTAGAAATCAAAAAGATGTTGATGCAATTATGCTGGAAATTGATGGAACCCCAAACAAGTCGAAACTTGGGGCAAATGCTATACTAGGAGTTTCTTTGAGCGTATGCAGAGCTGGTGCTGGCGCCAAGGGACTACCATTATATAGGCACATCCAAGAATTATCAGGCACAAAGGAACTTGTTATGCCAGTTCCTGCTTTTAATGTCATAAATGGGGGTAGCCATGCTGGAAATAATCTAGCAATGCAAGAATTTATGATCTTACCAGTAGGTGCCACATCTTTCGCTGAGGCACTTCGCATGGGAAGTGAAGGTTAGCTGCTGCTTCTTAAGCACTGCAATTTTCTTTTCTATCATTATGGTGTTAGTTTATTATGCATTTTGCACCTCGTGCATACGCTCGTTTCTGACATATATACAAGATTTATAGACAGTCTCTACGTCTTCCTCCTTTTCATATAATTAAGCCTGTCATTAGTTGGTAGCTTGACTTTAGTATTTTCTTATGAACAGTTTATCACACACTGAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGGTTTGCTCCAAATGTTCAAGATAACAGAGAGGGACTGGTGCTGCTTATTGATGCCATTGAAAAGGCTGGTTATACAGGAAAGGTATATGATACTAAATGTTTTTTTTTTTTTTTTTTGGAATAGTGTATAGTAGTTGTTTTATTACCTATCCTGCTAAGACAAGAAATTTAATCTGACACAGATCAAAATAGGAATGGATGTTGCAGCTTCAGAGTTTTTCACTAAAGATGGAACATATGATCTGAACTTCAAGAAACAGCCAAATGATGGAGCTCATGTTCACTCAGCTCATGGCCTTGGCGAACTCTATAAACAGTTCGTCAAAGATTTTCCCATTGTTTCCATCGAGGACCCCTTTGACCAAGATGATTGGAGTTCCTGGGCTTCCCTACAATCTTCTGTTGATATCCAACTTGTGGGAGATGACTTGCTTGTAACAAATCCAAAGAGAATAGCTGAGGCCATTAAGAAAAAGGCTTGTAATGCTTTACTTCTGAAGGTAAGCAGCCTCTTATTTCATTCTTACCTTTCTCTCTGTTATAGACTAATTCTAGGATTCCGGTTGGTCCATAAAACTCTTTTTTTTTTTTTTTTTCCTTTTCCTGAAATATAAGGTTCTCCATTGATTTAGGTTGACCAGTCTGTCTCTGCAGTTAGAAACATGTGATTTTGATACTGCAATTTGCTCATATGTTAGAAATTTCTTCTGGCAGGTTAACCAGATTGGATCAGTGACTGAATCAATTCAAGCTGCACTTGATTCAAAGGCTGCTGGTTGGGGTGTGATGGTCAGCCACCGAAGTGGTGAAACTGAGGATAATTTTATTGCTGATCTTTCTGTTGGCTTAGCTAGTCGACAGGTTTCATTCATCTTCAGCTCTTCTATCAATAATTATAAGTCAAATACTATAAGTCAATAACTTTATGTCGATAATGTTTGTATGTTGTTGGTATAACATTCATAACTGAATATTGTGTTACTCTTCTATAGATCAAAACTGGAGCCCCATGCCGAAGTGAACGGTTAGCTAAATATAATCAGGTAAAGTTTGATCAAATCATAACTTCCAAAACTATTGTCCTTGTTAAATGTTCCTGACTGTTTGTCTCGAAATGTTTGTAAGTTTCACTAATTGTACTGTTCTAATTCTTCTCGATATCAATTTGGATGCAGCTTCTTCGCATTGAAGAAGAACTAGGCAATGTTCGCTATGCCGGTGAGTCTTTCAGATCACCTTAGGAGGTAAAAGTTCAAGGTGACAGGAATGATGTATCAATTTTAGGTTGAAGGTTGGCTTGATAGAAGAAAATGTAGAGGATGATTTCTGCAGAAACAATATGCAGAAGTTTCCTGTCCGAGGTGTTCGAATCTGACACGTTACAAAATTGATTTGAGTGTTACCATGAGAGTATTTAAATTTTTGTTTTGAACTATCCATGGAAATGTTCGTTTCAAAATAATGCAGCTCTTTCTGATTTGCA

mRNA sequence

ATGGCTGCTGCTCTTCCTTCCCCACCCACCATTAACATGAACCTCCTCGACAAAACCCTCCTTCCTTCCAAACCTCAATCTTTCATTACTTCAACAACTTCTTCGAAGCCTCTTCTTCGCTCCCATGGTGTTCGCTGCTCTGTTGCTGTGGCGCCTTCTGCGTCGACCAAGGAGTCGAGGGAATTTAAGTTGAAGTCCCTGAAGGCCCGGCAGATCATTGACAGCAGAGGCAATCCGACGGTCGAGGTTGATTTGATTACTGATGATCTCTACCGATCCGCTGTCCCCAGTGGGGCCTCCACTGGGATCTATGAGGCTTTGGAGCTTCGAGATGGAGATAAAAGCGTTTATGGAGGTAAAGGCGTGCTTACTGCTGTTAAGAACATCAATGAAATCTTGGCCCCAAAGCTCGTCGGCGTCGATATCAGAAATCAAAAAGATGTTGATGCAATTATGCTGGAAATTGATGGAACCCCAAACAAGTCGAAACTTGGGGCAAATGCTATACTAGGAGTTTCTTTGAGCGTATGCAGAGCTGGTGCTGGCGCCAAGGGACTACCATTATATAGGCACATCCAAGAATTATCAGGCACAAAGGAACTTGTTATGCCAGTTCCTGCTTTTAATGTCATAAATGGGGGTAGCCATGCTGGAAATAATCTAGCAATGCAAGAATTTATGATCTTACCAGTAGGTGCCACATCTTTCGCTGAGGCACTTCGCATGGGAAGTGAAGTTTATCACACACTGAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGGTTTGCTCCAAATGTTCAAGATAACAGAGAGGGACTGGTGCTGCTTATTGATGCCATTGAAAAGGCTGGTTATACAGGAAAGATCAAAATAGGAATGGATGTTGCAGCTTCAGAGTTTTTCACTAAAGATGGAACATATGATCTGAACTTCAAGAAACAGCCAAATGATGGAGCTCATGTTCACTCAGCTCATGGCCTTGGCGAACTCTATAAACAGTTCGTCAAAGATTTTCCCATTGTTTCCATCGAGGACCCCTTTGACCAAGATGATTGGAGTTCCTGGGCTTCCCTACAATCTTCTGTTGATATCCAACTTGTGGGAGATGACTTGCTTGTAACAAATCCAAAGAGAATAGCTGAGGCCATTAAGAAAAAGGCTTGTAATGCTTTACTTCTGAAGGTTAACCAGATTGGATCAGTGACTGAATCAATTCAAGCTGCACTTGATTCAAAGGCTGCTGGTTGGGGTGTGATGGTCAGCCACCGAAGTGGTGAAACTGAGGATAATTTTATTGCTGATCTTTCTGTTGGCTTAGCTAGTCGACAGATCAAAACTGGAGCCCCATGCCGAAGTGAACGGTTAGCTAAATATAATCAGCTTCTTCGCATTGAAGAAGAACTAGGCAATGTTCGCTATGCCGGTGAGTCTTTCAGATCACCTTAG

Coding sequence (CDS)

ATGGCTGCTGCTCTTCCTTCCCCACCCACCATTAACATGAACCTCCTCGACAAAACCCTCCTTCCTTCCAAACCTCAATCTTTCATTACTTCAACAACTTCTTCGAAGCCTCTTCTTCGCTCCCATGGTGTTCGCTGCTCTGTTGCTGTGGCGCCTTCTGCGTCGACCAAGGAGTCGAGGGAATTTAAGTTGAAGTCCCTGAAGGCCCGGCAGATCATTGACAGCAGAGGCAATCCGACGGTCGAGGTTGATTTGATTACTGATGATCTCTACCGATCCGCTGTCCCCAGTGGGGCCTCCACTGGGATCTATGAGGCTTTGGAGCTTCGAGATGGAGATAAAAGCGTTTATGGAGGTAAAGGCGTGCTTACTGCTGTTAAGAACATCAATGAAATCTTGGCCCCAAAGCTCGTCGGCGTCGATATCAGAAATCAAAAAGATGTTGATGCAATTATGCTGGAAATTGATGGAACCCCAAACAAGTCGAAACTTGGGGCAAATGCTATACTAGGAGTTTCTTTGAGCGTATGCAGAGCTGGTGCTGGCGCCAAGGGACTACCATTATATAGGCACATCCAAGAATTATCAGGCACAAAGGAACTTGTTATGCCAGTTCCTGCTTTTAATGTCATAAATGGGGGTAGCCATGCTGGAAATAATCTAGCAATGCAAGAATTTATGATCTTACCAGTAGGTGCCACATCTTTCGCTGAGGCACTTCGCATGGGAAGTGAAGTTTATCACACACTGAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGGTTTGCTCCAAATGTTCAAGATAACAGAGAGGGACTGGTGCTGCTTATTGATGCCATTGAAAAGGCTGGTTATACAGGAAAGATCAAAATAGGAATGGATGTTGCAGCTTCAGAGTTTTTCACTAAAGATGGAACATATGATCTGAACTTCAAGAAACAGCCAAATGATGGAGCTCATGTTCACTCAGCTCATGGCCTTGGCGAACTCTATAAACAGTTCGTCAAAGATTTTCCCATTGTTTCCATCGAGGACCCCTTTGACCAAGATGATTGGAGTTCCTGGGCTTCCCTACAATCTTCTGTTGATATCCAACTTGTGGGAGATGACTTGCTTGTAACAAATCCAAAGAGAATAGCTGAGGCCATTAAGAAAAAGGCTTGTAATGCTTTACTTCTGAAGGTTAACCAGATTGGATCAGTGACTGAATCAATTCAAGCTGCACTTGATTCAAAGGCTGCTGGTTGGGGTGTGATGGTCAGCCACCGAAGTGGTGAAACTGAGGATAATTTTATTGCTGATCTTTCTGTTGGCTTAGCTAGTCGACAGATCAAAACTGGAGCCCCATGCCGAAGTGAACGGTTAGCTAAATATAATCAGCTTCTTCGCATTGAAGAAGAACTAGGCAATGTTCGCTATGCCGGTGAGTCTTTCAGATCACCTTAG

Protein sequence

MAAALPSPPTINMNLLDKTLLPSKPQSFITSTTSSKPLLRSHGVRCSVAVAPSASTKESREFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLTAVKNINEILAPKLVGVDIRNQKDVDAIMLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLLRIEEELGNVRYAGESFRSP

Homology

[Close]

BLAST of Spg021948 vs. NCBI nr
Match: KAG6587623.1 (Enolase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 896.7 bits (2316), Expect = 8.7e-257
Identity = 460/491 (93.69%), Postives = 473/491 (96.33%), Query Frame = 0


Query: 3   AALPSPPTINMNLLDKTLLPSKPQS---FITSTTSSKPLLRSHGVRCSVAVAPSASTKES 62
           A+LPSPPT   NLL+K+LLPSKPQS    I S++SSKP LRSHGVRCSVAVAPSASTK S
Sbjct: 2   ASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKP-LRSHGVRCSVAVAPSASTKAS 61

Query: 63  REFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGG 122
           REFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK+VYGG
Sbjct: 62  REFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG 121

Query: 123 KGVLTAVKNINEILAPKLVGVDIRNQKDVDAIMLEIDGTPNKSKLGANAILGVSLSVCRA 182
           KGVLTAVKNIN+ILAPKLVGVD+RNQ+DVDAIMLEIDGT NKSKLGANAILGVSLSVCRA
Sbjct: 122 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 181

Query: 183 GAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEA 242
           GAGAKGLPLYRHIQELS TKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA SFAEA
Sbjct: 182 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 241

Query: 243 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIK 302
           LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKAGYTGKIK
Sbjct: 242 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 301

Query: 303 IGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQD 362
           IGMDVAASEFFTKDG YDLNFKKQPNDGAHVHSAHGLGELYKQFV DFPIVSIEDPFDQD
Sbjct: 302 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 361

Query: 363 DWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVTESIQAALD 422
           DWSSWASLQSSV +Q+VGDDLLVTNPKRIAEAI+KKACNALLLKVNQIGSVTESIQAALD
Sbjct: 362 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 421

Query: 423 SKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLLRIEEELGN 482
           SKAAGWGVMVSHRSGETEDNFIADLSVGLAS QIKTGAPCRSERLAKYNQLLRIEEELGN
Sbjct: 422 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN 481

Query: 483 VRYAGESFRSP 491
           VRYAGESFRSP
Sbjct: 482 VRYAGESFRSP 491

[Close]

BLAST of Spg021948 vs. NCBI nr
Match: KAG7021584.1 (Enolase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 896.7 bits (2316), Expect = 8.7e-257
Identity = 460/491 (93.69%), Postives = 473/491 (96.33%), Query Frame = 0


Query: 3   AALPSPPTINMNLLDKTLLPSKPQS---FITSTTSSKPLLRSHGVRCSVAVAPSASTKES 62
           A+LPSPPT   NLL+K+LLPSKPQS    I S++SSKP LRSHGVRCSVAVAPSASTK S
Sbjct: 59  ASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKP-LRSHGVRCSVAVAPSASTKAS 118

Query: 63  REFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGG 122
           REFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK+VYGG
Sbjct: 119 REFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG 178

Query: 123 KGVLTAVKNINEILAPKLVGVDIRNQKDVDAIMLEIDGTPNKSKLGANAILGVSLSVCRA 182
           KGVLTAVKNIN+ILAPKLVGVD+RNQ+DVDAIMLEIDGT NKSKLGANAILGVSLSVCRA
Sbjct: 179 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 238

Query: 183 GAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEA 242
           GAGAKGLPLYRHIQELS TKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA SFAEA
Sbjct: 239 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 298

Query: 243 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIK 302
           LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKAGYTGKIK
Sbjct: 299 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 358

Query: 303 IGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQD 362
           IGMDVAASEFFTKDG YDLNFKKQPNDGAHVHSAHGLGELYKQFV DFPIVSIEDPFDQD
Sbjct: 359 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 418

Query: 363 DWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVTESIQAALD 422
           DWSSWASLQSSV +Q+VGDDLLVTNPKRIAEAI+KKACNALLLKVNQIGSVTESIQAALD
Sbjct: 419 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 478

Query: 423 SKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLLRIEEELGN 482
           SKAAGWGVMVSHRSGETEDNFIADLSVGLAS QIKTGAPCRSERLAKYNQLLRIEEELGN
Sbjct: 479 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN 538

Query: 483 VRYAGESFRSP 491
           VRYAGESFRSP
Sbjct: 539 VRYAGESFRSP 548

[Close]

BLAST of Spg021948 vs. NCBI nr
Match: XP_022931709.1 (enolase 1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 894.4 bits (2310), Expect = 4.3e-256
Identity = 459/491 (93.48%), Postives = 472/491 (96.13%), Query Frame = 0


Query: 3   AALPSPPTINMNLLDKTLLPSKPQS---FITSTTSSKPLLRSHGVRCSVAVAPSASTKES 62
           A+LPSPPT   NLL+K+LLPSKPQS    I S++SSKP LRSHGVRCSVAVAPSASTK S
Sbjct: 2   ASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKP-LRSHGVRCSVAVAPSASTKAS 61

Query: 63  REFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGG 122
            EFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK+VYGG
Sbjct: 62  SEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG 121

Query: 123 KGVLTAVKNINEILAPKLVGVDIRNQKDVDAIMLEIDGTPNKSKLGANAILGVSLSVCRA 182
           KGVLTAVKNIN+ILAPKLVGVD+RNQ+DVDAIMLEIDGT NKSKLGANAILGVSLSVCRA
Sbjct: 122 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 181

Query: 183 GAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEA 242
           GAGAKGLPLYRHIQELS TKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA SFAEA
Sbjct: 182 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 241

Query: 243 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIK 302
           LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKAGYTGKIK
Sbjct: 242 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 301

Query: 303 IGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQD 362
           IGMDVAASEFFTKDG YDLNFKKQPNDGAHVHSAHGLGELYKQFV DFPIVSIEDPFDQD
Sbjct: 302 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 361

Query: 363 DWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVTESIQAALD 422
           DWSSWASLQSSV +Q+VGDDLLVTNPKRIAEAI+KKACNALLLKVNQIGSVTESIQAALD
Sbjct: 362 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 421

Query: 423 SKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLLRIEEELGN 482
           SKAAGWGVMVSHRSGETEDNFIADLSVGLAS QIKTGAPCRSERLAKYNQLLRIEEELGN
Sbjct: 422 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN 481

Query: 483 VRYAGESFRSP 491
           VRYAGESFRSP
Sbjct: 482 VRYAGESFRSP 491

[Close]

BLAST of Spg021948 vs. NCBI nr
Match: XP_022965656.1 (enolase 1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 893.3 bits (2307), Expect = 9.6e-256
Identity = 458/491 (93.28%), Postives = 470/491 (95.72%), Query Frame = 0


Query: 3   AALPSPPTINMNLLDKTLLPSKPQS---FITSTTSSKPLLRSHGVRCSVAVAPSASTKES 62
           A+LPSPPT   NLL+K+LLPSKPQS    I S++SSKP LRSHGVRCSVA APS STK S
Sbjct: 2   ASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKP-LRSHGVRCSVAAAPSVSTKAS 61

Query: 63  REFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGG 122
           REFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGG
Sbjct: 62  REFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGG 121

Query: 123 KGVLTAVKNINEILAPKLVGVDIRNQKDVDAIMLEIDGTPNKSKLGANAILGVSLSVCRA 182
           KGVLTAVKNIN+ILAPKLVGVD+RNQ+DVDAIMLEIDGT NKSKLGANAILGVSLSVCRA
Sbjct: 122 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 181

Query: 183 GAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEA 242
           GAGAKGLPLYRHIQELS TKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA SFAEA
Sbjct: 182 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 241

Query: 243 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIK 302
           LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKAGYTGKIK
Sbjct: 242 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 301

Query: 303 IGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQD 362
           IGMDVAASEFFTKDG YDLNFKKQPNDGAHVHSAHGLGELYKQFV DFPIVSIEDPFDQD
Sbjct: 302 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 361

Query: 363 DWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVTESIQAALD 422
           DWSSWASLQSSV +Q+VGDDLLVTNPKRIAEAI+KKACNALLLKVNQIGSVTESIQAALD
Sbjct: 362 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 421

Query: 423 SKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLLRIEEELGN 482
           SKAAGWGVMVSHRSGETEDNFIADLSVGLAS QIKTGAPCRSERL KYNQLLRIEEELGN
Sbjct: 422 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLTKYNQLLRIEEELGN 481

Query: 483 VRYAGESFRSP 491
           VRYAGESFRSP
Sbjct: 482 VRYAGESFRSP 491

[Close]

BLAST of Spg021948 vs. NCBI nr
Match: XP_038879680.1 (enolase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 891.3 bits (2302), Expect = 3.7e-255
Identity = 455/492 (92.48%), Postives = 476/492 (96.75%), Query Frame = 0


Query: 3   AALPSPPTINMNLLDKTLLPSKPQSFI----TSTTSSKPLLRSHGVRCSVAVAPSASTKE 62
           A+LPSPPT  MNLL+K+LLPS PQSFI    +S++SSKP LRSH +RCSVAVAPS STK 
Sbjct: 2   ASLPSPPT--MNLLEKSLLPSSPQSFISSIPSSSSSSKP-LRSHAIRCSVAVAPSVSTKA 61

Query: 63  SREFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYG 122
           S+EFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK+VYG
Sbjct: 62  SKEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKTVYG 121

Query: 123 GKGVLTAVKNINEILAPKLVGVDIRNQKDVDAIMLEIDGTPNKSKLGANAILGVSLSVCR 182
           GKGVLTAVKNIN+ILAPKLVGVD+RNQ++VDAIMLEIDGTPNKSKLGANAILGVSLSVCR
Sbjct: 122 GKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSLSVCR 181

Query: 183 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAE 242
           AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA SFAE
Sbjct: 182 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAISFAE 241

Query: 243 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 302
           ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDN+EGLVLLIDAIEKAGYTGKI
Sbjct: 242 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNKEGLVLLIDAIEKAGYTGKI 301

Query: 303 KIGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 362
           KIGMDVAASEFFTK+G YDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ
Sbjct: 302 KIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 361

Query: 363 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVTESIQAAL 422
           DDWSSWASLQSSVDIQLVGDDLLVTNPKRIA+ I+KKACNALLLKVNQIGSVTESIQAAL
Sbjct: 362 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAKGIQKKACNALLLKVNQIGSVTESIQAAL 421

Query: 423 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLLRIEEELG 482
           DSKAAGWGVMVSHRSGETEDNFIADLSVGLAS QIKTGAPCRSERLAKYNQLLRIEEE+G
Sbjct: 422 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEEIG 481

Query: 483 NVRYAGESFRSP 491
           ++RYAG SFRSP
Sbjct: 482 SIRYAGASFRSP 490

[Close]

BLAST of Spg021948 vs. ExPASy Swiss-Prot
Match: Q9C9C4 (Enolase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ENO1 PE=1 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 9.4e-230
Identity = 398/451 (88.25%), Postives = 425/451 (94.24%), Query Frame = 0


Query: 40  RSHGVRCSVAVAPSASTKESREFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGA 99
           R  GV+CSV          ++E ++K +KARQIIDSRGNPTVEVDLITDDLYRSAVPSGA
Sbjct: 36  RRSGVQCSVV---------AKECRVKGVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGA 95

Query: 100 STGIYEALELRDGDKSVYGGKGVLTAVKNINEILAPKLVGVDIRNQKDVDAIMLEIDGTP 159
           STGIYEALELRDGDKSVYGGKGVL A+KNINE++APKL+GVD+RNQ DVDA+MLE+DGTP
Sbjct: 96  STGIYEALELRDGDKSVYGGKGVLQAIKNINELVAPKLIGVDVRNQADVDALMLELDGTP 155

Query: 160 NKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGN 219
           NKSKLGANAILGVSLSVCRAGAGAKG+PLY+HIQE SGTKELVMPVPAFNVINGGSHAGN
Sbjct: 156 NKSKLGANAILGVSLSVCRAGAGAKGVPLYKHIQETSGTKELVMPVPAFNVINGGSHAGN 215

Query: 220 NLAMQEFMILPVGATSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNR 279
           +LAMQEFMILPVGATSF+EA +MGSEVYHTLKGIIK KYGQDACNVGDEGGFAPNVQDNR
Sbjct: 216 SLAMQEFMILPVGATSFSEAFQMGSEVYHTLKGIIKTKYGQDACNVGDEGGFAPNVQDNR 275

Query: 280 EGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGEL 339
           EGLVLLIDAIEKAGYTGKIKIGMDVAASEFF KDG YDLNFKKQPNDGAHV SA  L +L
Sbjct: 276 EGLVLLIDAIEKAGYTGKIKIGMDVAASEFFMKDGRYDLNFKKQPNDGAHVLSAESLADL 335

Query: 340 YKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNA 399
           Y++F+KDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKK++CNA
Sbjct: 336 YREFIKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKQSCNA 395

Query: 400 LLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPC 459
           LLLKVNQIG+VTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLAS QIKTGAPC
Sbjct: 396 LLLKVNQIGTVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPC 455

Query: 460 RSERLAKYNQLLRIEEELGNVRYAGESFRSP 491
           RSERL+KYNQLLRIEEELGNVRYAGE+FRSP
Sbjct: 456 RSERLSKYNQLLRIEEELGNVRYAGEAFRSP 477

[Close]

BLAST of Spg021948 vs. ExPASy Swiss-Prot
Match: Q54RK5 (Enolase A OS=Dictyostelium discoideum OX=44689 GN=enoA PE=3 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 6.4e-170
Identity = 303/431 (70.30%), Postives = 358/431 (83.06%), Query Frame = 0


Query: 64  LKSLKARQIIDSRGNPTVEVDLITD-----DLYRSAVPSGASTGIYEALELRDGDKSVYG 123
           +KS+KAR+I++SRGNPTVEVDL T+       +R+AVPSGASTGIYEA+ELRDGDKS Y 
Sbjct: 4   IKSIKAREILNSRGNPTVEVDLYTEKDGVVSSFRAAVPSGASTGIYEAVELRDGDKSRYL 63

Query: 124 GKGVLTAVKNINEILAPKLVGVDIRNQKDVDAIMLEIDGTPNKSKLGANAILGVSLSVCR 183
           GKGVL A+KNI E++ P ++G  + +Q  +D +M+++DGTPNK KLGANAIL VSL+VCR
Sbjct: 64  GKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPNKGKLGANAILAVSLAVCR 123

Query: 184 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAE 243
           AGA  + LPLY++I E++GTK + +PVPAFNVINGGSHAGN LAMQEFMILPVGA  F E
Sbjct: 124 AGAADRNLPLYKYISEIAGTK-MRLPVPAFNVINGGSHAGNKLAMQEFMILPVGAKDFNE 183

Query: 244 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 303
           A RMGSEVYH LK +I  +YGQDA NVGDEGGFAP +Q N+EGL LL  AIEKAGYTG +
Sbjct: 184 AYRMGSEVYHNLKNVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLV 243

Query: 304 KIGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 363
           KIGMD AASEF  ++G YDL+FK + NDG+ V S   LG+LY++F+K++PI+SIEDPFDQ
Sbjct: 244 KIGMDCAASEFKVENG-YDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQ 303

Query: 364 DDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVTESIQAAL 423
           DDW S+  L +SVDIQ+VGDDLLVTNP+RI   I+KKACNALLLKVNQIGSVTESI+AAL
Sbjct: 304 DDWESYTKLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAAL 363

Query: 424 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLLRIEEELG 483
           DSK A WGVMVSHRSGETED FIADL VGL + QIKTGAPCRSERLAKYNQL+RI EELG
Sbjct: 364 DSKNASWGVMVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELG 423

Query: 484 -NVRYAGESFR 489
            N  YAG +FR
Sbjct: 424 ENHNYAGLTFR 432

[Close]

BLAST of Spg021948 vs. ExPASy Swiss-Prot
Match: P15007 (Enolase OS=Drosophila melanogaster OX=7227 GN=Eno PE=1 SV=2)

HSP 1 Score: 598.6 bits (1542), Expect = 6.4e-170
Identity = 299/440 (67.95%), Postives = 362/440 (82.27%), Query Frame = 0


Query: 55  STKESREFKLKSLKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGD 114
           ST +S E  +K++KARQI DSRGNPTVEVDL T+  L+R+AVPSGASTG++EALELRD D
Sbjct: 61  STCDSNEMTIKAIKARQIYDSRGNPTVEVDLTTELGLFRAAVPSGASTGVHEALELRDND 120

Query: 115 KSVYGGKGVLTAVKNINEILAPKLV--GVDIRNQKDVDAIMLEIDGTPNKSKLGANAILG 174
           K+ Y GK VL AV ++N+ L P+L+   +D+ +Q  +D  M+++DGT NKSK GANAILG
Sbjct: 121 KANYHGKSVLKAVGHVNDTLGPELIKANLDVVDQASIDNFMIKLDGTENKSKFGANAILG 180

Query: 175 VSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPV 234
           VSL+V +AGA  KG+PLY+HI +L+G KE+++PVPAFNVINGGSHAGN LAMQEFMILP 
Sbjct: 181 VSLAVAKAGAAKKGVPLYKHIADLAGNKEIILPVPAFNVINGGSHAGNKLAMQEFMILPT 240

Query: 235 GATSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEK 294
           GATSF EA++MGSEVYH LK +IKAK+G DA  VGDEGGFAPN+Q N+E L L+ DAI K
Sbjct: 241 GATSFTEAMKMGSEVYHHLKNVIKAKFGLDATAVGDEGGFAPNIQSNKEALNLISDAIAK 300

Query: 295 AGYTGKIKIGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVS 354
           AGYTGKI+IGMDVAASEF+ KDG YDL+FK + +D +    A  L  LY++F+KDFPIVS
Sbjct: 301 AGYTGKIEIGMDVAASEFY-KDGQYDLDFKNEKSDKSQWLPADKLANLYQEFIKDFPIVS 360

Query: 355 IEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVT 414
           IEDPFDQD W +W++L    DIQ+VGDDL VTNPKRIA A++KKACN LLLKVNQIG+VT
Sbjct: 361 IEDPFDQDHWEAWSNLTGCTDIQIVGDDLTVTNPKRIATAVEKKACNCLLLKVNQIGTVT 420

Query: 415 ESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLL 474
           ESI A L +K  GWG MVSHRSGETED+FI DL VGL++ QIKTGAPCRSERLAKYNQ+L
Sbjct: 421 ESIAAHLLAKKNGWGTMVSHRSGETEDSFIGDLVVGLSTGQIKTGAPCRSERLAKYNQIL 480

Query: 475 RIEEELG-NVRYAGESFRSP 491
           RIEEE+G  V++AG+SFR P
Sbjct: 481 RIEEEIGAGVKFAGKSFRKP 499

[Close]

BLAST of Spg021948 vs. ExPASy Swiss-Prot
Match: P42894 (Enolase OS=Neocallimastix frontalis OX=4757 PE=3 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 1.9e-169
Identity = 305/434 (70.28%), Postives = 360/434 (82.95%), Query Frame = 0


Query: 64  LKSLKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKSVYGGKGV 123
           +  + ARQI DSRGNPTVEV++ TD  L+R+AVPSGASTG++EALELRDG K+ Y GKGV
Sbjct: 3   ITKVHARQIFDSRGNPTVEVEVTTDKGLFRAAVPSGASTGVHEALELRDGIKADYVGKGV 62

Query: 124 LTAVKNINEILAPKLV--GVDIRNQKDVDAIMLEIDGTPNKSKLGANAILGVSLSVCRAG 183
           L AV+N+N+ +AP LV   +D++NQK VD  +L++DGTPNKSKLGANAILGVSL+V RAG
Sbjct: 63  LKAVENVNKTIAPALVAANLDVKNQKAVDDFLLKLDGTPNKSKLGANAILGVSLAVARAG 122

Query: 184 AGAKGLPLYRHIQELSGTK-ELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEA 243
           A  KG+PLY+H+ EL+G K   ++PVP+ NV+NGGSHAGN LAMQEFMILP GA SF EA
Sbjct: 123 AADKGVPLYQHLGELAGNKGPWILPVPSMNVLNGGSHAGNKLAMQEFMILPTGAKSFTEA 182

Query: 244 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIK 303
           L+MGSEVYH LK +IKAKYGQDACNVGDEGGFAPN+QDN+EGL LL +AI KAGYTGK+K
Sbjct: 183 LKMGSEVYHALKSVIKAKYGQDACNVGDEGGFAPNIQDNKEGLELLNEAIAKAGYTGKVK 242

Query: 304 IGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQD 363
           IGMDVA+SEF+ KDG YDL+FK   +D +   S   LG+ YK+   ++PIVSIEDP+DQD
Sbjct: 243 IGMDVASSEFY-KDGKYDLDFKNPNSDPSKWISGEELGQFYKEITSEYPIVSIEDPYDQD 302

Query: 364 DWSSWASLQSSVD--IQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVTESIQAA 423
           D+ SW+  ++ +   IQ+VGDDL VTNPKRIA AI+KKACN LLLKVNQIG+V+ESIQAA
Sbjct: 303 DFESWSKFRADMQDKIQIVGDDLTVTNPKRIAMAIEKKACNGLLLKVNQIGTVSESIQAA 362

Query: 424 LDSKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLLRIEEEL 483
           LD+   GWGVMVSHRSGETED FIADL VGL S QIKTGAPCRSERLAKYNQLLRIEEEL
Sbjct: 363 LDAFNDGWGVMVSHRSGETEDTFIADLVVGLKSGQIKTGAPCRSERLAKYNQLLRIEEEL 422

Query: 484 G-NVRYAGESFRSP 491
           G N  YAGE+FR P
Sbjct: 423 GANATYAGENFRRP 435

[Close]

BLAST of Spg021948 vs. ExPASy Swiss-Prot
Match: Q27527 (Enolase OS=Caenorhabditis elegans OX=6239 GN=enol-1 PE=1 SV=3)

HSP 1 Score: 593.6 bits (1529), Expect = 2.1e-168
Identity = 305/431 (70.77%), Postives = 353/431 (81.90%), Query Frame = 0


Query: 64  LKSLKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKSVYGGKGV 123
           +  + ARQI DSRGNPTVEVDL T+  ++R+AVPSGASTG++EALELRDGDK+V+ GKGV
Sbjct: 3   ITKIHARQIYDSRGNPTVEVDLFTEKGVFRAAVPSGASTGVHEALELRDGDKAVHLGKGV 62

Query: 124 LTAVKNINEILAPKLV--GVDIRNQKDVDAIMLEIDGTPNKSKLGANAILGVSLSVCRAG 183
           L AV NINE +AP L+  G D+  QKD+D  M+ +DG+ NK  LGANAILGVSL+V +AG
Sbjct: 63  LKAVSNINEKIAPALIAKGFDVTAQKDIDDFMMALDGSENKGNLGANAILGVSLAVAKAG 122

Query: 184 AGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEAL 243
           A  KGLPLY++I EL+GT ++V+PVPAFNVINGGSHAGN LAMQEFMILPVGA+SFAEA+
Sbjct: 123 AVHKGLPLYKYIAELAGTGKVVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFAEAM 182

Query: 244 RMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKI 303
           RMGSEVYH LK  IK +YG DA  VGDEGGFAPN+QDN+EGL LL  AI+KAGYTGKI I
Sbjct: 183 RMGSEVYHHLKAEIKKRYGLDATAVGDEGGFAPNIQDNKEGLDLLNTAIDKAGYTGKISI 242

Query: 304 GMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQDD 363
           GMDVAASEFF KDG YDL+FK   +D +   S   L ELY+ F+K++P+VSIED FDQDD
Sbjct: 243 GMDVAASEFF-KDGKYDLDFKNPASDSSKWLSGEQLTELYQSFIKEYPVVSIEDAFDQDD 302

Query: 364 WSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVTESIQAALDS 423
           W +W     +  IQLVGDDL VTNPKRI  AI KK+CN LLLKVNQIGSVTESI+AA  S
Sbjct: 303 WDNWGKFHGATSIQLVGDDLTVTNPKRIQTAIDKKSCNCLLLKVNQIGSVTESIEAAKLS 362

Query: 424 KAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLLRIEEELG-N 483
           +A GWGVMVSHRSGETED FIADL VGLA+ QIKTGAPCRSERLAKYNQLLRIEEELG +
Sbjct: 363 RANGWGVMVSHRSGETEDTFIADLVVGLATGQIKTGAPCRSERLAKYNQLLRIEEELGAD 422

Query: 484 VRYAGESFRSP 491
             YAG +FR+P
Sbjct: 423 AVYAGHNFRNP 432

[Close]

BLAST of Spg021948 vs. ExPASy TrEMBL
Match: A0A6J1EV05 (Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111437858 PE=3 SV=1)

HSP 1 Score: 894.4 bits (2310), Expect = 2.1e-256
Identity = 459/491 (93.48%), Postives = 472/491 (96.13%), Query Frame = 0


Query: 3   AALPSPPTINMNLLDKTLLPSKPQS---FITSTTSSKPLLRSHGVRCSVAVAPSASTKES 62
           A+LPSPPT   NLL+K+LLPSKPQS    I S++SSKP LRSHGVRCSVAVAPSASTK S
Sbjct: 2   ASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKP-LRSHGVRCSVAVAPSASTKAS 61

Query: 63  REFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGG 122
            EFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK+VYGG
Sbjct: 62  SEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKNVYGG 121

Query: 123 KGVLTAVKNINEILAPKLVGVDIRNQKDVDAIMLEIDGTPNKSKLGANAILGVSLSVCRA 182
           KGVLTAVKNIN+ILAPKLVGVD+RNQ+DVDAIMLEIDGT NKSKLGANAILGVSLSVCRA
Sbjct: 122 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 181

Query: 183 GAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEA 242
           GAGAKGLPLYRHIQELS TKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA SFAEA
Sbjct: 182 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 241

Query: 243 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIK 302
           LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKAGYTGKIK
Sbjct: 242 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 301

Query: 303 IGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQD 362
           IGMDVAASEFFTKDG YDLNFKKQPNDGAHVHSAHGLGELYKQFV DFPIVSIEDPFDQD
Sbjct: 302 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 361

Query: 363 DWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVTESIQAALD 422
           DWSSWASLQSSV +Q+VGDDLLVTNPKRIAEAI+KKACNALLLKVNQIGSVTESIQAALD
Sbjct: 362 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 421

Query: 423 SKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLLRIEEELGN 482
           SKAAGWGVMVSHRSGETEDNFIADLSVGLAS QIKTGAPCRSERLAKYNQLLRIEEELGN
Sbjct: 422 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGN 481

Query: 483 VRYAGESFRSP 491
           VRYAGESFRSP
Sbjct: 482 VRYAGESFRSP 491

[Close]

BLAST of Spg021948 vs. ExPASy TrEMBL
Match: A0A6J1HM99 (Phosphopyruvate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111465484 PE=3 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 4.7e-256
Identity = 458/491 (93.28%), Postives = 470/491 (95.72%), Query Frame = 0


Query: 3   AALPSPPTINMNLLDKTLLPSKPQS---FITSTTSSKPLLRSHGVRCSVAVAPSASTKES 62
           A+LPSPPT   NLL+K+LLPSKPQS    I S++SSKP LRSHGVRCSVA APS STK S
Sbjct: 2   ASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKP-LRSHGVRCSVAAAPSVSTKAS 61

Query: 63  REFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGG 122
           REFKLKS+KARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGG
Sbjct: 62  REFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGG 121

Query: 123 KGVLTAVKNINEILAPKLVGVDIRNQKDVDAIMLEIDGTPNKSKLGANAILGVSLSVCRA 182
           KGVLTAVKNIN+ILAPKLVGVD+RNQ+DVDAIMLEIDGT NKSKLGANAILGVSLSVCRA
Sbjct: 122 KGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRA 181

Query: 183 GAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEA 242
           GAGAKGLPLYRHIQELS TKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA SFAEA
Sbjct: 182 GAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAASFAEA 241

Query: 243 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIK 302
           LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKAGYTGKIK
Sbjct: 242 LRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKAGYTGKIK 301

Query: 303 IGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQD 362
           IGMDVAASEFFTKDG YDLNFKKQPNDGAHVHSAHGLGELYKQFV DFPIVSIEDPFDQD
Sbjct: 302 IGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSIEDPFDQD 361

Query: 363 DWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVTESIQAALD 422
           DWSSWASLQSSV +Q+VGDDLLVTNPKRIAEAI+KKACNALLLKVNQIGSVTESIQAALD
Sbjct: 362 DWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTESIQAALD 421

Query: 423 SKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLLRIEEELGN 482
           SKAAGWGVMVSHRSGETEDNFIADLSVGLAS QIKTGAPCRSERL KYNQLLRIEEELGN
Sbjct: 422 SKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLTKYNQLLRIEEELGN 481

Query: 483 VRYAGESFRSP 491
           VRYAGESFRSP
Sbjct: 482 VRYAGESFRSP 491

[Close]

BLAST of Spg021948 vs. ExPASy TrEMBL
Match: A0A5D3DYS6 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00060 PE=3 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 4.5e-251
Identity = 455/498 (91.37%), Postives = 470/498 (94.38%), Query Frame = 0


Query: 3   AALPSPPTINMNLLDKTLLPSKPQSFI----------TSTTSSKPLLRSHGVRCSVAVAP 62
           A+LPSPPT  MNLL+K L  S PQSFI          +S++SSKP LRS  +RCSVAVAP
Sbjct: 2   ASLPSPPT--MNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKP-LRSPTIRCSVAVAP 61

Query: 63  SASTKESREFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDG 122
           S ST  S+EFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDG
Sbjct: 62  SVSTNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDG 121

Query: 123 DKSVYGGKGVLTAVKNINEILAPKLVGVDIRNQKDVDAIMLEIDGTPNKSKLGANAILGV 182
           DK+VYGGKGVLTAVKNIN+ILAPKLVGVD+RNQ++VDAIMLEIDGTPNKSKLGANAILGV
Sbjct: 122 DKTVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGV 181

Query: 183 SLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG 242
           SLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG
Sbjct: 182 SLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG 241

Query: 243 ATSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKA 302
           A+SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKA
Sbjct: 242 ASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKA 301

Query: 303 GYTGKIKIGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSI 362
           GYTGKIKIGMDVAASEFFTK G YDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSI
Sbjct: 302 GYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSI 361

Query: 363 EDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVTE 422
           EDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAE I+KKACNALLLKVNQIGSVTE
Sbjct: 362 EDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTE 421

Query: 423 SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLLR 482
           SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLAS QIKTGAPCRSERLAKYNQLLR
Sbjct: 422 SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR 481

Query: 483 IEEELGNVRYAGESFRSP 491
           IEEELG+VRYAG SFRSP
Sbjct: 482 IEEELGSVRYAGASFRSP 496

[Close]

BLAST of Spg021948 vs. ExPASy TrEMBL
Match: A0A5A7US84 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003390 PE=3 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 1.0e-250
Identity = 454/498 (91.16%), Postives = 470/498 (94.38%), Query Frame = 0


Query: 3   AALPSPPTINMNLLDKTLLPSKPQSFI----------TSTTSSKPLLRSHGVRCSVAVAP 62
           A+LPSPPT  MNLL+K L  S PQSFI          +S++SSKP LRS  +RCSVAVAP
Sbjct: 2   ASLPSPPT--MNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKP-LRSPTIRCSVAVAP 61

Query: 63  SASTKESREFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDG 122
           S ST  S+EFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDG
Sbjct: 62  SVSTNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDG 121

Query: 123 DKSVYGGKGVLTAVKNINEILAPKLVGVDIRNQKDVDAIMLEIDGTPNKSKLGANAILGV 182
           DK+VYGGKGVLTAVKNI +ILAPKLVGVD+RNQ++VDAIMLEIDGTPNKSKLGANAILGV
Sbjct: 122 DKTVYGGKGVLTAVKNIKDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGV 181

Query: 183 SLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG 242
           SLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG
Sbjct: 182 SLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG 241

Query: 243 ATSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKA 302
           A+SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKA
Sbjct: 242 ASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKA 301

Query: 303 GYTGKIKIGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSI 362
           GYTGKIKIGMDVAASEFFTK+G YDLNFKKQPNDGAHVHSAH LGELYKQFVKDFPIVSI
Sbjct: 302 GYTGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSI 361

Query: 363 EDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVTE 422
           EDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAE I+KKACNALLLKVNQIGSVTE
Sbjct: 362 EDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTE 421

Query: 423 SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLLR 482
           SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLAS QIKTGAPCRSERLAKYNQLLR
Sbjct: 422 SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR 481

Query: 483 IEEELGNVRYAGESFRSP 491
           IEEELG+VRYAG SFRSP
Sbjct: 482 IEEELGSVRYAGASFRSP 496

[Close]

BLAST of Spg021948 vs. ExPASy TrEMBL
Match: A0A6J1BZW1 (Phosphopyruvate hydratase OS=Momordica charantia OX=3673 GN=LOC111007005 PE=3 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 1.0e-250
Identity = 452/497 (90.95%), Postives = 467/497 (93.96%), Query Frame = 0


Query: 3   AALPSPPTINMNLLDKTLLPSKPQSFI---------TSTTSSKPLLRSHGVRCSVAVAPS 62
           A+L SPP   MNLL  TLLPSK  S I         +S++SS   LRS  VRCSVAVAPS
Sbjct: 2   ASLSSPPA--MNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAPS 61

Query: 63  ASTKESREFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGD 122
           A+TK S+E+KLKS+KARQIIDSRGNPTVEVDL+TDDLYRSAVPSGASTGIYEALELRDGD
Sbjct: 62  AATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGD 121

Query: 123 KSVYGGKGVLTAVKNINEILAPKLVGVDIRNQKDVDAIMLEIDGTPNKSKLGANAILGVS 182
           KSVYGGKGVLTAVKNIN+ILAPKLVGVD+RNQ+DVDAIMLEIDGT NKSKLGANAILGVS
Sbjct: 122 KSVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVS 181

Query: 183 LSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA 242
           LSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA
Sbjct: 182 LSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA 241

Query: 243 TSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAG 302
           TSFAEALRMGSEVYH LKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAG
Sbjct: 242 TSFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAG 301

Query: 303 YTGKIKIGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIE 362
           YTGKIKIGMDVAASEFFTKDG YDLNFKKQPNDGAHVHSAHGLGELYKQF+KDFPIVSIE
Sbjct: 302 YTGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIE 361

Query: 363 DPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVTES 422
           DPFDQDDWSSW+SLQSSVDIQLVGDDLLVTNPKRIAEAI KKAC ALLLKVNQIGSVTES
Sbjct: 362 DPFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACTALLLKVNQIGSVTES 421

Query: 423 IQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLLRI 482
           IQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLAS QIKTGAPCRSERLAKYNQLLRI
Sbjct: 422 IQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRI 481

Query: 483 EEELGNVRYAGESFRSP 491
           EEELG+VRYAGESFRSP
Sbjct: 482 EEELGSVRYAGESFRSP 496

[Close]

BLAST of Spg021948 vs. TAIR 10
Match: AT1G74030.1 (enolase 1 )

HSP 1 Score: 797.3 bits (2058), Expect = 6.7e-231
Identity = 398/451 (88.25%), Postives = 425/451 (94.24%), Query Frame = 0


Query: 40  RSHGVRCSVAVAPSASTKESREFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGA 99
           R  GV+CSV          ++E ++K +KARQIIDSRGNPTVEVDLITDDLYRSAVPSGA
Sbjct: 36  RRSGVQCSVV---------AKECRVKGVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGA 95

Query: 100 STGIYEALELRDGDKSVYGGKGVLTAVKNINEILAPKLVGVDIRNQKDVDAIMLEIDGTP 159
           STGIYEALELRDGDKSVYGGKGVL A+KNINE++APKL+GVD+RNQ DVDA+MLE+DGTP
Sbjct: 96  STGIYEALELRDGDKSVYGGKGVLQAIKNINELVAPKLIGVDVRNQADVDALMLELDGTP 155

Query: 160 NKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGN 219
           NKSKLGANAILGVSLSVCRAGAGAKG+PLY+HIQE SGTKELVMPVPAFNVINGGSHAGN
Sbjct: 156 NKSKLGANAILGVSLSVCRAGAGAKGVPLYKHIQETSGTKELVMPVPAFNVINGGSHAGN 215

Query: 220 NLAMQEFMILPVGATSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNR 279
           +LAMQEFMILPVGATSF+EA +MGSEVYHTLKGIIK KYGQDACNVGDEGGFAPNVQDNR
Sbjct: 216 SLAMQEFMILPVGATSFSEAFQMGSEVYHTLKGIIKTKYGQDACNVGDEGGFAPNVQDNR 275

Query: 280 EGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGEL 339
           EGLVLLIDAIEKAGYTGKIKIGMDVAASEFF KDG YDLNFKKQPNDGAHV SA  L +L
Sbjct: 276 EGLVLLIDAIEKAGYTGKIKIGMDVAASEFFMKDGRYDLNFKKQPNDGAHVLSAESLADL 335

Query: 340 YKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNA 399
           Y++F+KDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKK++CNA
Sbjct: 336 YREFIKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKQSCNA 395

Query: 400 LLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPC 459
           LLLKVNQIG+VTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLAS QIKTGAPC
Sbjct: 396 LLLKVNQIGTVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPC 455

Query: 460 RSERLAKYNQLLRIEEELGNVRYAGESFRSP 491
           RSERL+KYNQLLRIEEELGNVRYAGE+FRSP
Sbjct: 456 RSERLSKYNQLLRIEEELGNVRYAGEAFRSP 477

[Close]

BLAST of Spg021948 vs. TAIR 10
Match: AT2G36530.1 (Enolase )

HSP 1 Score: 575.9 bits (1483), Expect = 3.2e-164
Identity = 298/435 (68.51%), Postives = 350/435 (80.46%), Query Frame = 0


Query: 67  LKARQIIDSRGNPTVEVDLITDDLYR--SAVPSGASTGIYEALELRDGDKSVYGGKGVLT 126
           +KARQI DSRGNPTVEVD+ T +  +  +AVPSGASTGIYEALELRDG  S Y GKGV  
Sbjct: 7   VKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDGG-SDYLGKGVSK 66

Query: 127 AVKNINEILAPKLVGVDIRNQKDVDAIML-EIDGTPN-----KSKLGANAILGVSLSVCR 186
           AV N+N I+ P L+G D   Q  +D  M+ E+DGT N     K KLGANAIL VSL+VC+
Sbjct: 67  AVGNVNNIIGPALIGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVSLAVCK 126

Query: 187 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAE 246
           AGA   G+PLY+HI  L+G  ++V+PVPAFNVINGGSHAGN LAMQEFMILPVGA SF E
Sbjct: 127 AGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAASFKE 186

Query: 247 ALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 306
           A++MG EVYH LK +IK KYGQDA NVGDEGGFAPN+Q+N+EGL LL  AIEKAGYTGK+
Sbjct: 187 AMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGYTGKV 246

Query: 307 KIGMDVAASEFFTKDGTYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQ 366
            IGMDVAASEF+++D TYDLNFK++ N+G+   S   L +LYK FV ++PIVSIEDPFDQ
Sbjct: 247 VIGMDVAASEFYSEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSIEDPFDQ 306

Query: 367 DDWSSWASL--QSSVDIQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVTESIQA 426
           DDW  +A +  +   ++Q+VGDDLLVTNPKR+A+AI +K+CNALLLKVNQIGSVTESI+A
Sbjct: 307 DDWEHYAKMTTECGTEVQIVGDDLLVTNPKRVAKAIAEKSCNALLLKVNQIGSVTESIEA 366

Query: 427 ALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLLRIEEE 486
              SK AGWGVM SHRSGETED FIADL+VGL++ QIKTGAPCRSERLAKYNQLLRIEEE
Sbjct: 367 VKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQLLRIEEE 426

Query: 487 LGN-VRYAGESFRSP 491
           LG+   YAG +FR P
Sbjct: 427 LGSEAIYAGVNFRKP 440

[Close]

BLAST of Spg021948 vs. TAIR 10
Match: AT2G29560.1 (cytosolic enolase )

HSP 1 Score: 458.8 bits (1179), Expect = 5.6e-129
Identity = 233/428 (54.44%), Postives = 309/428 (72.20%), Query Frame = 0


Query: 64  LKSLKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKSVYGGKGV 123
           +  +KARQI+DSRG PTVEVDL T+  ++R++VPSG S+G YEA+ELRDGDK +Y G  V
Sbjct: 47  ITKVKARQILDSRGIPTVEVDLHTNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSV 106

Query: 124 LTAVKNINEILAPKLVGVDIRNQKDVDAIMLEIDGTPNKSKLGANAILGVSLSVCRAGAG 183
             AVKNINE ++  L+G+D + Q  +D  M+++D T  KS+LGANAIL VS++ C+AGA 
Sbjct: 107 AKAVKNINEKISEALIGMDPKLQGQIDQAMIDLDKTEKKSELGANAILAVSIAACKAGAA 166

Query: 184 AKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEALRM 243
            K +PL +H+ +LSG   +V+PVPAF V++GG HA N  A+QE MILP+GA+ F EAL+ 
Sbjct: 167 EKEVPLCKHLSDLSGRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGASRFEEALQW 226

Query: 244 GSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGM 303
           GSE YH LK +I  K G   CNVG++GG AP++   +EGL L+ +AI + GY  KIKI +
Sbjct: 227 GSETYHHLKAVISEKNGGLGCNVGEDGGLAPDISSLKEGLELVKEAINRTGYNDKIKIAI 286

Query: 304 DVAASEFFTKDGT-YDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIEDPFDQDDW 363
           D+AA+ F    GT YDL+ K     G +  SA  + ++YK+   D+PIVSIEDPFD++DW
Sbjct: 287 DIAATNFCL--GTKYDLDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKEDW 346

Query: 364 SSWASLQSSVDIQLVGDDLLVTNPKRIAEAIKKKACNALLLKVNQIGSVTESIQAALDSK 423
                  S    Q+VGDDLL++N KR+  AI++ +CNALLLKVNQIG+VTE+I+    ++
Sbjct: 347 EHTKYFSSLGICQVVGDDLLMSNSKRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMAR 406

Query: 424 AAGWGVMVSHRSGETEDNFIADLSVGLASRQIKTGAPCRSERLAKYNQLLRIEEELGN-V 483
            A WGV+ SHR GETED+FI+DLSVGLA+  IK GAPCR ER  KYNQLLRIEEELG+  
Sbjct: 407 DAQWGVVTSHRCGETEDSFISDLSVGLATGVIKAGAPCRGERTMKYNQLLRIEEELGDQA 466

Query: 484 RYAGESFR 489
            YAGE ++
Sbjct: 467 VYAGEDWK 472

The following BLAST results are available for this feature:

BLAST of Spg021948 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6587623.1	8.7e-257	93.69	Enolase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7021584.1	8.7e-257	93.69	Enolase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022931709.1	4.3e-256	93.48	enolase 1, chloroplastic [Cucurbita moschata]	[more]
XP_022965656.1	9.6e-256	93.28	enolase 1, chloroplastic [Cucurbita maxima]	[more]
XP_038879680.1	3.7e-255	92.48	enolase 1, chloroplastic [Benincasa hispida]	[more]

BLAST of Spg021948 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C9C4	9.4e-230	88.25	Enolase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ENO1 PE=1 SV=1	[more]
Q54RK5	6.4e-170	70.30	Enolase A OS=Dictyostelium discoideum OX=44689 GN=enoA PE=3 SV=1	[more]
P15007	6.4e-170	67.95	Enolase OS=Drosophila melanogaster OX=7227 GN=Eno PE=1 SV=2	[more]
P42894	1.9e-169	70.28	Enolase OS=Neocallimastix frontalis OX=4757 PE=3 SV=1	[more]
Q27527	2.1e-168	70.77	Enolase OS=Caenorhabditis elegans OX=6239 GN=enol-1 PE=1 SV=3	[more]

BLAST of Spg021948 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EV05	2.1e-256	93.48	Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111437858 PE=3 SV=...	[more]
A0A6J1HM99	4.7e-256	93.28	Phosphopyruvate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111465484 PE=3 SV=1	[more]
A0A5D3DYS6	4.5e-251	91.37	Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A5A7US84	1.0e-250	91.16	Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A6J1BZW1	1.0e-250	90.95	Phosphopyruvate hydratase OS=Momordica charantia OX=3673 GN=LOC111007005 PE=3 SV...	[more]

BLAST of Spg021948 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G74030.1	6.7e-231	88.25	enolase 1	[more]
AT2G36530.1	3.2e-164	68.51	Enolase	[more]
AT2G29560.1	5.6e-129	54.44	cytosolic enolase	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000941	Enolase	PRINTS	PR00148	ENOLASE	coord: 95..109 score: 88.38 coord: 429..446 score: 83.0 coord: 377..388 score: 78.16 coord: 400..414 score: 80.2 coord: 222..235 score: 75.87 coord: 165..181 score: 76.74
IPR000941	Enolase	TIGRFAM	TIGR01060	TIGR01060	coord: 65..486 e-value: 1.7E-164 score: 545.4
IPR000941	Enolase	SFLD	SFLDF00002	enolase	coord: 65..477 e-value: 0.0 score: 620.7
IPR000941	Enolase	PANTHER	PTHR11902	ENOLASE	coord: 30..490
IPR000941	Enolase	HAMAP	MF_00318	Enolase	coord: 62..477 score: 43.339058
IPR000941	Enolase	CDD	cd03313	enolase	coord: 66..476 e-value: 0.0 score: 737.369
IPR020810	Enolase, C-terminal TIM barrel domain	SMART	SM01192	Enolase_C_3	coord: 200..490 e-value: 2.4E-191 score: 651.7
IPR020810	Enolase, C-terminal TIM barrel domain	PFAM	PF00113	Enolase_C	coord: 201..488 e-value: 6.4E-146 score: 485.2
IPR020811	Enolase, N-terminal	SMART	SM01193	Enolase_N_3	coord: 64..192 e-value: 9.2E-85 score: 297.6
IPR020811	Enolase, N-terminal	PFAM	PF03952	Enolase_N	coord: 65..192 e-value: 2.5E-55 score: 186.3
IPR029017	Enolase-like, N-terminal	GENE3D	3.30.390.10		coord: 58..185 e-value: 7.4E-56 score: 189.8
IPR029017	Enolase-like, N-terminal	SUPERFAMILY	54826	Enolase N-terminal domain-like	coord: 64..195
IPR036849	Enolase-like, C-terminal domain superfamily	GENE3D	3.20.20.120		coord: 186..490 e-value: 3.1E-152 score: 508.0
IPR036849	Enolase-like, C-terminal domain superfamily	SUPERFAMILY	51604	Enolase C-terminal domain-like	coord: 200..485
None	No IPR available	SFLD	SFLDG00178	enolase	coord: 65..477 e-value: 0.0 score: 620.7
None	No IPR available	PANTHER	PTHR11902:SF42	ENOLASE 1, CHLOROPLASTIC	coord: 30..490
IPR020809	Enolase, conserved site	PROSITE	PS00164	ENOLASE	coord: 400..413

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg021948.1	Spg021948.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006096	glycolytic process
biological_process	GO:0010090	trichome morphogenesis
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0000015	phosphopyruvate hydratase complex
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0004634	phosphopyruvate hydratase activity