Spg003635 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg003635
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	alpha-amylase/subtilisin inhibitor-like
Location	scaffold4: 45091708 .. 45092268 (+)
RNA-Seq Expression	Spg003635
Synteny	Spg003635

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGCTGCAAAAACTGTTTGCTAATTTGTGTGCATTTGCAGCCATATTCTCGAGCGCTGCAGGCATCCCAGTACTCGACACCAACGGGAAGCCTCTTGAATCCGGTCCCCAGTACTACATAAAGCCTGCAATTCCTGGTAGAGGTGGTGATCTCACTCTGGTTACAAGAAAAAACCAACGCAGTCCATGCCCAAAATACGTCGGTCAAGTAGAGAAACCGGAAATGAAAGGAACTCCGATTAACTTTACGTCGAATTCGGAGGTCCACGAAAGCACGATCATCCAAATTACGTTTCAAGCTCCGACTTCCTGCCCGGAGTCTCGGATGTGGGGGCTCGGAGAGAATGAGAACAGGACAAGTCGGAAGTTGATCGGGATGAGCCCGACGTTACTTGTACAATCTGTATTCCGGATTGTGGATAACAATCGCCTTTACCATTTGCTCTGGTGTCCTGATGTCCTCAATTGTGGAATGGCTGGTATCTTAGGTGAGGAGGGAGTACATTTGCTGGCCTTGGATGGCCCTGCGCTTCCTTTTGTTTTTGAGAGAGTTGTGTGA

mRNA sequence

ATGCAGCTGCAAAAACTGTTTGCTAATTTGTGTGCATTTGCAGCCATATTCTCGAGCGCTGCAGGCATCCCAGTACTCGACACCAACGGGAAGCCTCTTGAATCCGGTCCCCAGTACTACATAAAGCCTGCAATTCCTGGTAGAGGTGGTGATCTCACTCTGGTTACAAGAAAAAACCAACGCAGTCCATGCCCAAAATACGTCGGTCAAGTAGAGAAACCGGAAATGAAAGGAACTCCGATTAACTTTACGTCGAATTCGGAGGTCCACGAAAGCACGATCATCCAAATTACGTTTCAAGCTCCGACTTCCTGCCCGGAGTCTCGGATGTGGGGGCTCGGAGAGAATGAGAACAGGACAAGTCGGAAGTTGATCGGGATGAGCCCGACGTTACTTGTACAATCTGTATTCCGGATTGTGGATAACAATCGCCTTTACCATTTGCTCTGGTGTCCTGATGTCCTCAATTGTGGAATGGCTGGTATCTTAGGTGAGGAGGGAGTACATTTGCTGGCCTTGGATGGCCCTGCGCTTCCTTTTGTTTTTGAGAGAGTTGTGTGA

Coding sequence (CDS)

ATGCAGCTGCAAAAACTGTTTGCTAATTTGTGTGCATTTGCAGCCATATTCTCGAGCGCTGCAGGCATCCCAGTACTCGACACCAACGGGAAGCCTCTTGAATCCGGTCCCCAGTACTACATAAAGCCTGCAATTCCTGGTAGAGGTGGTGATCTCACTCTGGTTACAAGAAAAAACCAACGCAGTCCATGCCCAAAATACGTCGGTCAAGTAGAGAAACCGGAAATGAAAGGAACTCCGATTAACTTTACGTCGAATTCGGAGGTCCACGAAAGCACGATCATCCAAATTACGTTTCAAGCTCCGACTTCCTGCCCGGAGTCTCGGATGTGGGGGCTCGGAGAGAATGAGAACAGGACAAGTCGGAAGTTGATCGGGATGAGCCCGACGTTACTTGTACAATCTGTATTCCGGATTGTGGATAACAATCGCCTTTACCATTTGCTCTGGTGTCCTGATGTCCTCAATTGTGGAATGGCTGGTATCTTAGGTGAGGAGGGAGTACATTTGCTGGCCTTGGATGGCCCTGCGCTTCCTTTTGTTTTTGAGAGAGTTGTGTGA

Protein sequence

MQLQKLFANLCAFAAIFSSAAGIPVLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQRSPCPKYVGQVEKPEMKGTPINFTSNSEVHESTIIQITFQAPTSCPESRMWGLGENENRTSRKLIGMSPTLLVQSVFRIVDNNRLYHLLWCPDVLNCGMAGILGEEGVHLLALDGPALPFVFERVV

Homology

[Close]

BLAST of Spg003635 vs. NCBI nr
Match: XP_022150757.1 (alpha-amylase/subtilisin inhibitor-like [Momordica charantia])

HSP 1 Score: 218.4 bits (555), Expect = 5.2e-53
Identity = 113/188 (60.11%), Postives = 132/188 (70.21%), Query Frame = 0


Query: 1   MQLQKLFANLCAFAAIFSSAAGIPVLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQ 60
           M LQKL A LC FAAIFS+AA +PVLDTNG  + SG QYYIKPAI GRGG LTLV RKN 
Sbjct: 1   MVLQKLVA-LCLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNG 60

Query: 61  RSPCPKYVGQVEKPEMKGTPINFTSNSEVHESTIIQITFQAPTSCPESRMWGLGENENRT 120
           + PCPKYVGQVE P+ KGTP+ F      +E  +  ITFQ   SC +  MWGLG+ EN +
Sbjct: 61  K-PCPKYVGQVEHPDQKGTPVKFMLAPVTNEGWLAHITFQTSDSCSKLTMWGLGDRENVS 120

Query: 121 SRKLIGMSPTLLVQSVFRIVDNNRLYHLLWCPDV---LNCGMAGILGEEGVHLLALDGPA 180
            R+LI     LLV+ +F+IV +N LYH+LWC        CG AGIL E+GV LLALDGP 
Sbjct: 121 GRRLITSGSELLVEVLFKIVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSLLALDGPE 180

Query: 181 LPFVFERV 186
           LPFVFE+V
Sbjct: 181 LPFVFEKV 186

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BLAST of Spg003635 vs. NCBI nr
Match: XP_023545477.1 (miraculin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 148.3 bits (373), Expect = 6.6e-32
Identity = 81/185 (43.78%), Postives = 110/185 (59.46%), Query Frame = 0


Query: 6   LFANLCAFAAIFSSAAGIPVLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQRSPCP 65
           +F+ L  F AI S+A   PVLDT+G+PL+ G +YYIKPAI    G+LTL +R N  +PCP
Sbjct: 8   IFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSN--APCP 67

Query: 66  KYVGQVEKPEMK-GTPINFT----SNSEVHESTIIQITFQAPTSCPESRMWGLGENENRT 125
            YVGQ        G P+ FT        + ES    + FQA ++C  S  W + E E+ T
Sbjct: 68  LYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESAT 127

Query: 126 SRKLIGMSPTLLVQSVFRIVDNNRLYHLLWCPDVL---NCGMAGILGEEGVHLLALDGPA 183
            R+ +G+        +FRI  NN +Y+++WCP+++    CG AGIL E+GV LLALDG A
Sbjct: 128 GRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNA 187

[Close]

BLAST of Spg003635 vs. NCBI nr
Match: XP_022999136.1 (miraculin-like [Cucurbita maxima])

HSP 1 Score: 146.7 bits (369), Expect = 1.9e-31
Identity = 81/185 (43.78%), Postives = 108/185 (58.38%), Query Frame = 0


Query: 6   LFANLCAFAAIFSSAAGIPVLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQRSPCP 65
           +F+ L  F AI S+A   PVLDT+G+PL  G +YYIKPAI    G+LTL  R N  +PCP
Sbjct: 8   IFSYLLVFLAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRSN--APCP 67

Query: 66  KYVGQVEKPEMK-GTPINFT----SNSEVHESTIIQITFQAPTSCPESRMWGLGENENRT 125
            YVGQ        G P+ FT        + ES    + FQA ++C  S  W + E E+ T
Sbjct: 68  LYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESAT 127

Query: 126 SRKLIGMSPTLLVQSVFRIVDNNRLYHLLWCPDVL---NCGMAGILGEEGVHLLALDGPA 183
            R+ +G+        +FRI  NN +Y+++WCP+++    CG AGIL E+GV LLALDG A
Sbjct: 128 GRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNA 187

[Close]

BLAST of Spg003635 vs. NCBI nr
Match: XP_022946294.1 (miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita moschata])

HSP 1 Score: 146.0 bits (367), Expect = 3.3e-31
Identity = 81/185 (43.78%), Postives = 109/185 (58.92%), Query Frame = 0


Query: 6   LFANLCAFAAIFSSAAGIPVLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQRSPCP 65
           +F+ L  F AI S+A   PVLDT+G+PL  G +YYIKPAI    G+LTL +R N  +PCP
Sbjct: 8   IFSFLLVFMAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSN--APCP 67

Query: 66  KYVGQVEKPEMK-GTPINFT----SNSEVHESTIIQITFQAPTSCPESRMWGLGENENRT 125
            YVGQ        G P+ FT        + ES    + FQA ++C  S  W + E E+ T
Sbjct: 68  LYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESAT 127

Query: 126 SRKLIGMSPTLLVQSVFRIVDNNRLYHLLWCPDVL---NCGMAGILGEEGVHLLALDGPA 183
            R+ +G+        +FRI  NN +Y+++WCP+++    CG AGIL E+GV LLALDG A
Sbjct: 128 GRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNA 187

[Close]

BLAST of Spg003635 vs. NCBI nr
Match: XP_038888526.1 (kunitz type trypsin inhibitor 106-like [Benincasa hispida])

HSP 1 Score: 144.8 bits (364), Expect = 7.3e-31
Identity = 80/185 (43.24%), Postives = 107/185 (57.84%), Query Frame = 0


Query: 6   LFANLCAFAAIFSSAAGIPVLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQRSPCP 65
           +F   C F AI S+A   PVLDT+ +PL  G +YYIKPAI   GG+LTL +R N  +PCP
Sbjct: 10  IFGYACLFMAITSTAQLPPVLDTDSQPLRRGVEYYIKPAITDVGGNLTLKSRSN--APCP 69

Query: 66  KYVGQVEKPEMK-GTPINFT----SNSEVHESTIIQITFQAPTSCPESRMWGLGENENRT 125
            +VGQ        G P+ FT        + ES  + I F+A ++C  S  W + E E+ T
Sbjct: 70  LFVGQEPVTSTNIGLPVTFTPIVAGEDIIEESRSLNIVFEALSTCVTSTQWRVDEAESDT 129

Query: 126 SRKLIGMSPTLLVQSVFRIVDNNRLYHLLWCPDVL---NCGMAGILGEEGVHLLALDGPA 183
            R+ +G+        +FRI  NN +Y+++WCP ++    CG AGIL E GV LLALDG A
Sbjct: 130 GRRFVGIGNDDGPSGIFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVENGVRLLALDGDA 189

[Close]

BLAST of Spg003635 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.5e-23
Identity = 66/177 (37.29%), Postives = 94/177 (53.11%), Query Frame = 0


Query: 25  VLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQRSPCPKYVGQVEKPEMKGTPINFT 84
           V+DT+G+P+E   +Y+I+PAI G GG  TLVT      PCP +VG        G  + FT
Sbjct: 27  VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT---GNGPCPLHVGLDNTEGTLGVAVKFT 86

Query: 85  ------SNSEVHESTIIQITFQAPTSCPESRMWGLGENENRTSRKLI--GMSPTLLVQ-S 144
                  + +V  +  +++TF   TSC +S  W LGE +  + R+LI  G       Q +
Sbjct: 87  PFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLGEKDATSGRRLIVTGRDNGAGSQGN 146

Query: 145 VFRIVDNNR--LYHLLWCPD------VLNCGMAGILGEEGVHLLALDGPALPFVFER 185
            FRIV       Y++ WCP        + CG  G++ E G +LLALDG ALP VF++
Sbjct: 147 FFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVIRENGKNLLALDGDALPVVFQK 200

[Close]

BLAST of Spg003635 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.0e-22
Identity = 68/177 (38.42%), Postives = 99/177 (55.93%), Query Frame = 0


Query: 25  VLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQRSPCPKYVG-QVEKPEMKGTPIN- 84
           V+DT+G+P+E+   Y+I+PAI G GG LTLVTR +    CP  VG   + P+     ++ 
Sbjct: 27  VIDTSGEPVENDEDYFIRPAITGNGGSLTLVTRNS----CPFNVGLDPDAPQGFAVLLSP 86

Query: 85  FTSNSEVHESTI---IQITFQAPTSCPESRMWGLGENENRTSRKLI--GMSPTLL--VQS 144
           F SN E  E  +   +++ FQA TSC +S  W LGE +  T R+ I  G   + +    +
Sbjct: 87  FVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERDATTGRRFIITGRDDSTVGSYGN 146

Query: 145 VFRIVD--NNRLYHLLWCPDVL------NCGMAGILGEEGVHLLALDGPALPFVFER 185
            FRIV   +  ++++ WCP  +       CG  GI+ E G  LLALDG ALP  F++
Sbjct: 147 FFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVRENGKILLALDGSALPVAFQK 199

[Close]

BLAST of Spg003635 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 8.5e-22
Identity = 66/197 (33.50%), Postives = 102/197 (51.78%), Query Frame = 0


Query: 6   LFANLCAFAAIFSSAAGIPVLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQRSPCP 65
           + A++C F  I ++     VLDT G+P+E   +Y+I+P I  +GG  T+V+R      CP
Sbjct: 12  ILAHVCLF--ITTTTIAQFVLDTVGEPVEGDEEYFIRPVITNKGGRSTMVSRNES---CP 71

Query: 66  KYVGQVEKPEMKGTPINFTSNSEVHE------STIIQITFQAPTSCPESRMWGLGENENR 125
            +VG       +G  + FT  +  H+      +  ++ITFQA +SC +S  W LGE + +
Sbjct: 72  LHVGLELTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEWRLGEKDTK 131

Query: 126 TSRKLIGMSPTLLVQ----SVFRIVDN--NRLYHLLWCPDVL------NCGMAGILGEEG 185
           + R+LI             + FRIV+     +Y++ WCP  +       CG   +L E G
Sbjct: 132 SGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGTVDMLNENG 191

[Close]

BLAST of Spg003635 vs. ExPASy Swiss-Prot
Match: P29421 (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 1.3e-14
Identity = 62/189 (32.80%), Postives = 85/189 (44.97%), Query Frame = 0


Query: 10  LCAFAAIFS-SAAGIPVLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQRSPCPKYV 69
           L   A  FS SAA  PV DT G  L +   YY+ PA PG GG LT+  R     PCP  V
Sbjct: 11  LSLLAISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV---LPCPLLV 70

Query: 70  GQVEKPEMKGTPINFT--------SNSEVHESTIIQITFQAPTSCPESRMWGLGENENRT 129
            Q      KG P+ FT         +  +  ST ++I F A T C +S  W +G+     
Sbjct: 71  AQETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVGDEPLTG 130

Query: 130 SRK-----LIGMSPTLLVQSVFRIVDNNRLYHLLWCPDVLNCGMAGILGEEGVHLLALDG 185
           +R+     LIG SP+   ++ FR+      Y L+ C D  +C   G+  +     L    
Sbjct: 131 ARRVVTGPLIGPSPSGR-ENAFRVEKYGGGYKLVSCRD--SCQDLGVSRDGARAWLGASQ 190

[Close]

BLAST of Spg003635 vs. ExPASy Swiss-Prot
Match: Q9LMU2 (Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.2e-12
Identity = 60/193 (31.09%), Postives = 87/193 (45.08%), Query Frame = 0


Query: 6   LFANLCAFAAIFSSAAGIPVLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQRSPCP 65
           L A   +   + + AA  PV D NGK L +G  YYI P I GRGG LT+   K +   CP
Sbjct: 10  LLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTE--TCP 69

Query: 66  KYVGQVEKPEMKGTPINFT---SNSEVHESTIIQITFQAPTSCPESRMWGLGENENRTSR 125
             V Q +    +G P+ F+    +  +  ST + I F +PTS     +W L   +  T +
Sbjct: 70  TSVIQDQFEVSQGLPVKFSPYDKSRTIPVSTDVNIKF-SPTS-----IWELANFDETTKQ 129

Query: 126 KLI------GMSPTLLVQSVFRIVDNNRLYHLLWCPDVLN-----CGMAGILGEEGVHLL 185
             I      G      V + F+I    + Y + +CP V N     C   G+  ++G   L
Sbjct: 130 WFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQDGKRRL 189

[Close]

BLAST of Spg003635 vs. ExPASy TrEMBL
Match: A0A6J1DBL5 (alpha-amylase/subtilisin inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111018817 PE=3 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 2.5e-53
Identity = 113/188 (60.11%), Postives = 132/188 (70.21%), Query Frame = 0


Query: 1   MQLQKLFANLCAFAAIFSSAAGIPVLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQ 60
           M LQKL A LC FAAIFS+AA +PVLDTNG  + SG QYYIKPAI GRGG LTLV RKN 
Sbjct: 1   MVLQKLVA-LCLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNG 60

Query: 61  RSPCPKYVGQVEKPEMKGTPINFTSNSEVHESTIIQITFQAPTSCPESRMWGLGENENRT 120
           + PCPKYVGQVE P+ KGTP+ F      +E  +  ITFQ   SC +  MWGLG+ EN +
Sbjct: 61  K-PCPKYVGQVEHPDQKGTPVKFMLAPVTNEGWLAHITFQTSDSCSKLTMWGLGDRENVS 120

Query: 121 SRKLIGMSPTLLVQSVFRIVDNNRLYHLLWCPDV---LNCGMAGILGEEGVHLLALDGPA 180
            R+LI     LLV+ +F+IV +N LYH+LWC        CG AGIL E+GV LLALDGP 
Sbjct: 121 GRRLITSGSELLVEVLFKIVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSLLALDGPE 180

Query: 181 LPFVFERV 186
           LPFVFE+V
Sbjct: 181 LPFVFEKV 186

[Close]

BLAST of Spg003635 vs. ExPASy TrEMBL
Match: A0A6J1KC85 (miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 9.4e-32
Identity = 81/185 (43.78%), Postives = 108/185 (58.38%), Query Frame = 0


Query: 6   LFANLCAFAAIFSSAAGIPVLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQRSPCP 65
           +F+ L  F AI S+A   PVLDT+G+PL  G +YYIKPAI    G+LTL  R N  +PCP
Sbjct: 8   IFSYLLVFLAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRSN--APCP 67

Query: 66  KYVGQVEKPEMK-GTPINFT----SNSEVHESTIIQITFQAPTSCPESRMWGLGENENRT 125
            YVGQ        G P+ FT        + ES    + FQA ++C  S  W + E E+ T
Sbjct: 68  LYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESAT 127

Query: 126 SRKLIGMSPTLLVQSVFRIVDNNRLYHLLWCPDVL---NCGMAGILGEEGVHLLALDGPA 183
            R+ +G+        +FRI  NN +Y+++WCP+++    CG AGIL E+GV LLALDG A
Sbjct: 128 GRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNA 187

[Close]

BLAST of Spg003635 vs. ExPASy TrEMBL
Match: A0A6J1G3G3 (miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 1.6e-31
Identity = 81/185 (43.78%), Postives = 109/185 (58.92%), Query Frame = 0


Query: 6   LFANLCAFAAIFSSAAGIPVLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQRSPCP 65
           +F+ L  F AI S+A   PVLDT+G+PL  G +YYIKPAI    G+LTL +R N  +PCP
Sbjct: 8   IFSFLLVFMAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSN--APCP 67

Query: 66  KYVGQVEKPEMK-GTPINFT----SNSEVHESTIIQITFQAPTSCPESRMWGLGENENRT 125
            YVGQ        G P+ FT        + ES    + FQA ++C  S  W + E E+ T
Sbjct: 68  LYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESAT 127

Query: 126 SRKLIGMSPTLLVQSVFRIVDNNRLYHLLWCPDVL---NCGMAGILGEEGVHLLALDGPA 183
            R+ +G+        +FRI  NN +Y+++WCP+++    CG AGIL E+GV LLALDG A
Sbjct: 128 GRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNA 187

[Close]

BLAST of Spg003635 vs. ExPASy TrEMBL
Match: A0A5D3D468 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520 PE=3 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 1.1e-29
Identity = 76/185 (41.08%), Postives = 103/185 (55.68%), Query Frame = 0


Query: 6   LFANLCAFAAIFSSAAGIPVLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQRSPCP 65
           +F   C F AI S+A   PVLDTNG+PL  G +YYI PAI   GG+LTL +R N  +PCP
Sbjct: 10  IFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRSN--APCP 69

Query: 66  KYVGQVEKPEMK-GTPINF----TSNSEVHESTIIQITFQAPTSCPESRMWGLGENENRT 125
            +VGQ        G P+ F         + E T + I F+A ++C  S  W +   E+ T
Sbjct: 70  LFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDATESDT 129

Query: 126 SRKLIGMSPTLLVQSVFRIVDNNRLYHLLWCPDVL---NCGMAGILGEEGVHLLALDGPA 183
            R+ +G+        +F I  +N  Y+++WCP ++    CG AGIL E GV L+ALDG A
Sbjct: 130 GRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDA 189

[Close]

BLAST of Spg003635 vs. ExPASy TrEMBL
Match: A0A5A7VDV8 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530 PE=3 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 1.1e-29
Identity = 76/185 (41.08%), Postives = 103/185 (55.68%), Query Frame = 0


Query: 6   LFANLCAFAAIFSSAAGIPVLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQRSPCP 65
           +F   C F AI S+A   PVLDTNG+PL  G +YYI PAI   GG+LTL +R N  +PCP
Sbjct: 8   IFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRSN--APCP 67

Query: 66  KYVGQVEKPEMK-GTPINF----TSNSEVHESTIIQITFQAPTSCPESRMWGLGENENRT 125
            +VGQ        G P+ F         + E T + I F+A ++C  S  W +   E+ T
Sbjct: 68  LFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDATESDT 127

Query: 126 SRKLIGMSPTLLVQSVFRIVDNNRLYHLLWCPDVL---NCGMAGILGEEGVHLLALDGPA 183
            R+ +G+        +F I  +N  Y+++WCP ++    CG AGIL E GV L+ALDG A
Sbjct: 128 GRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDA 187

[Close]

BLAST of Spg003635 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 74.7 bits (182), Expect = 8.7e-14
Identity = 60/193 (31.09%), Postives = 87/193 (45.08%), Query Frame = 0


Query: 6   LFANLCAFAAIFSSAAGIPVLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQRSPCP 65
           L A   +   + + AA  PV D NGK L +G  YYI P I GRGG LT+   K +   CP
Sbjct: 10  LLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTE--TCP 69

Query: 66  KYVGQVEKPEMKGTPINFT---SNSEVHESTIIQITFQAPTSCPESRMWGLGENENRTSR 125
             V Q +    +G P+ F+    +  +  ST + I F +PTS     +W L   +  T +
Sbjct: 70  TSVIQDQFEVSQGLPVKFSPYDKSRTIPVSTDVNIKF-SPTS-----IWELANFDETTKQ 129

Query: 126 KLI------GMSPTLLVQSVFRIVDNNRLYHLLWCPDVLN-----CGMAGILGEEGVHLL 185
             I      G      V + F+I    + Y + +CP V N     C   G+  ++G   L
Sbjct: 130 WFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQDGKRRL 189

[Close]

BLAST of Spg003635 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 60.8 bits (146), Expect = 1.3e-09
Identity = 56/196 (28.57%), Postives = 87/196 (44.39%), Query Frame = 0


Query: 7   FANLCAFAAIFSSAAGIPVLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRKNQRSPCPK 66
           F  + A  A+ +S A   V+D +G  +     YY+ P I GRGG LTL  R  Q  PCP 
Sbjct: 12  FYLVLALTAVLASNAYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQ--PCPY 71

Query: 67  YVGQVEKPEMKGTPINFTS----NSEVHESTIIQI-TFQAPTSCPESRMWGLGENENRTS 126
            + Q      +G P+ F++     + V ES  + I T    T C +S  W +GE ++   
Sbjct: 72  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 131

Query: 127 RKLIGMSPTL------LVQSVFRIVDNNR-LYHLLWCPDVLN-----CGMAGI-LGEEGV 185
           +  +   P         ++S F+I  +    Y  ++CP   +     C   GI + E GV
Sbjct: 132 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 191

[Close]

BLAST of Spg003635 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.4e-08
Identity = 53/184 (28.80%), Postives = 83/184 (45.11%), Query Frame = 0


Query: 7   FANLCAFAAIFSSAAGIP------VLDTNGKPLESGPQYYIKPAIPGRGGDLTLVTRK-N 66
           F  L   +AIF++A+         VLD  G P++S  QYYI PA  G GG L    R  +
Sbjct: 8   FITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLS 67

Query: 67  QRSPCPKY-VGQVEKPEMKGTPINF----TSNSEVHESTIIQITFQAPT-SCPESRMWGL 126
            +  C    + Q   P + G P+ F    T    V  S  + + F +    CP+S++W +
Sbjct: 68  TQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRI 127

Query: 127 GEN-ENRTSRKLIGMSPTLLVQSVFRIVDNNRLYHLLWCP--DVLNCGMAGI-LGEEGVH 174
             + + R S   IG        S F+I ++   Y L++CP   ++ C    + + + GV 
Sbjct: 128 DHSVQLRKSFVSIG-GQKGKGNSWFQIQEDGDAYKLMYCPISSIVACINVSLEIDDHGVR 187

The following BLAST results are available for this feature:

BLAST of Spg003635 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150757.1	5.2e-53	60.11	alpha-amylase/subtilisin inhibitor-like [Momordica charantia]	[more]
XP_023545477.1	6.6e-32	43.78	miraculin-like [Cucurbita pepo subsp. pepo]	[more]
XP_022999136.1	1.9e-31	43.78	miraculin-like [Cucurbita maxima]	[more]
XP_022946294.1	3.3e-31	43.78	miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita mo...	[more]
XP_038888526.1	7.3e-31	43.24	kunitz type trypsin inhibitor 106-like [Benincasa hispida]	[more]

BLAST of Spg003635 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A072TH68	1.5e-23	37.29	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
G7LCV7	1.0e-22	38.42	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
G7LCV1	8.5e-22	33.50	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
P29421	1.3e-14	32.80	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R...	[more]
Q9LMU2	1.2e-12	31.09	Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1	[more]

BLAST of Spg003635 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DBL5	2.5e-53	60.11	alpha-amylase/subtilisin inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1KC85	9.4e-32	43.78	miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1	[more]
A0A6J1G3G3	1.6e-31	43.78	miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1	[more]
A0A5D3D468	1.1e-29	41.08	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520...	[more]
A0A5A7VDV8	1.1e-29	41.08	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530...	[more]

BLAST of Spg003635 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	8.7e-14	31.09	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	1.3e-09	28.57	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	1.4e-08	28.80	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 64..84 score: 25.4 coord: 24..53 score: 42.48
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 24..186 e-value: 5.7E-24 score: 95.6
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 25..183 e-value: 3.4E-31 score: 108.5
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 5..184
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 25..41
None	No IPR available	GENE3D	2.80.10.50		coord: 23..186 e-value: 7.6E-37 score: 128.6
None	No IPR available	PANTHER	PTHR33107:SF40	TRYPSIN INHIBITOR A	coord: 5..184
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 24..184

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg003635.1	Spg003635.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004866	endopeptidase inhibitor activity