Spg003573 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg003573
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionE3 ubiquitin ligase
Locationscaffold4: 49020170 .. 49025145 (+)
RNA-Seq ExpressionSpg003573
SyntenySpg003573
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTTGGCGGCGAATTTTGTTAAGCACAAAATTCTCATCCAAACCCAAGAAGTCAACCGTCAACTCCACGCAGTGCTAACTGCTAAGAACGACGACGACCGATCAGCTTATCTTATAATTAATTCGAGACTCCAAATTAGAAATTAAAATGGAAAGTAAACCAAGTAAAAAAAAAAAAAAAAAAGAAAAAAAAGAAAGACCCAATTGAATTGGACTGACTCGTTGAGCTGTTGTTTCTAAGCCAATGACCTCCGTGGCGGTTGCCGTTAACGGCGTCCGCGGCGGCAAAGGCGGTGGCGGAAGCCGCCGCGCCGTCAGATGGGCCGTCGAGAATCTCTTGCCAACCGCCGATCGTTTCATTTTGGTTCACGTTATGCCCAAAATCACCTCCATTCCCACCCCAAGTACGTGCCTAAAAATTTTCTTTCTCAACTGTTTGAGAAAATAAAATCAAACAAGTTACGGCGACGCCTCAATTTCTCGATCTTTGATTCTGGGTTTTGTTTGTGTTTCTCAGTGGGGGATCGCGTCGCTGTCTCGGAACTCGATGCAGATGTTGTAGCACTTTATGTGCACGATGTGAAAGAGAAATATGAACAAGTTTTTGTCCCTTTCAAGAAGTTATGTAGAAGAGAAAAGGTAATTTGAAGCTCAAGAATTTTATTCCATTTTTTGTTTGAATTCCAGCCCCAAATTTATGGTAAACTGAAATGGGAATTTGTTCTGCAGTTTCTTCTTCTGGTAGAAACTTTGATTTTGGAGGATGACAATCCTGCTACTGCACTTTTGAGATATGCATCTGAATCTGGGATTAAACGCTTGGTTTTGGGTTCTTGTTTTAGAACTTGCATAGCAAGGTAATATTTGTGTCATTTCTGTTTTTATTTCTTCAAAGTTCTCACTGGTGAGGATCAGTGTTTTAAAAGGCGCGAGCCTAGGCGCAAGGCGCAACCTTAGCACCTCGCTTCGAAGAAGTCAAAGCCCTCGAGGCGCGCGCCTTAGGCGCCTCTGGGGCTTCACATTTTTATATATGTTTTAGGGTGGAGTATTGACATCATCATACTTGGTCATTCAGAGTGTCAGAAGGCATATTGACAATACTAAACTCATTGTTTTTTGTGGAGTATTGACATTTGACTCTATGTTGGAAGACTAATATTTGAACCTTATTTATCTTTTATCTATTTTTCTTGGTTAAAAACTTCATTACTTGCCATGAAAGTGTTTTATATACATGTATATATTTTTGTAAGTTTTTTTTCTATATAGTGCGCCTTAAAGAAAAAAGCCCGCACCTTTTTGTGCGTCTTGCGCCTAGGCCCCAGGAGGCCATTGCGCCTTAGTGCGCCTTGAGCCTTTTAAAACACTGGTGAGGATTCCACATTGAAAAGATAAGTGGAGACCTCACAAGTTATAAGATAGATGGATTACTCCACTCATTGTCAATTGGTTTTGAGATGGAACCTCATGTATCTAATATGGTATCAGAGTCCATGAAACCCAAACGGGTATTCAATCCATTAAAAAAAATGAGATCCGATCCAAGAATAGTGAACCCAAAGAGGCACCATCTTGAGGGGGCATGTGCCCCCACATTGAAAAGATAAGTGGACAAACCAATTGGTTTTGAGATGAACCTCATGTATCTAATACTTACTGTGATAACTAAAATTCATTAATGAAATGGTGATATGATGATGTTCATAATTTGGTGTTTAATTGGATTGGAAAATGCATAGACATGTAAGTTTATATGATATTGTTTACTCATTTCACATGATCTGAAAGCACTTACTCCTGTAGGAAGCTAAAAGGGGATTCAGTACCCTCTGCTGTTCTGAGAACTGCTCCTAGTTCTCTTGATATTTATGTTATATATAGGAGAAGAGTCGTTACAAGAATGGCTAGTACTGCTCTATCTACTGGTAATTTGCTGAAAGCACTTACTCCAGTCAGTTCTTTTTCTCCTCCTGATATGCAAATTTGTATGTAATTGTTTTTCACATTGAACTTTACAGAAACCGATTCGAGGCAGTGGATGCTTGGTGATACTGATTACTACAGGGGTTCAAGTGCCGTTAGTGAAAAATCATCTGGAACTTTTTCTTCTTCTTCCTTGTCGATTGTTCATCGAAGGGGCGATAGCCTCGAAGTTACTTCTACTGAGCAACCGAACTCTTTGAGTACTCTGACTGAGGAGGTTTTTATTTTTTCTTTGTGTTTTGAGCTTTGCTGTCCATCTTTGATTTAGTTTTCTTGATGGAATTTCGTCATCATTTCGAGGCGATGCTACAATGAGTTCTTAAATAACAGTCCTGAATTGCAGGAAGATATGCGGGCTGAAGTTGAATCACTGCAACTAGAATTAGAAACCACTGTTTCCTTGTACAAACAAGCTTGTGAAGAACTAGTACATGCTCAGAAGAAGGTGAGAGCTTGATCATTATATCGGTTTCGGCATAACGTTTTACGGGCCAAGGTTTTAGAATCTCCATGGGATTTCATGGAGACAATGAAGTTAAAGCTGAATCATGATGTTCTAAGATGCATTTGAGCATTTAAACCACACTCAATTCAATATGAAATGGAGTTGGCAGTGATAATTTTGTATGGTTTAGTAAACTTAAAGGAATGTTAAACCACTAATTGGCTTCATACAAATTAAAAGAAACAATGTGAATGCCTATTTGTGCTTCATTTTGCAGGTTCAGTCACTTACATCAGAATGCCTTGAAGAATCAAGAAGAGTAAATGCTGCCTTGGAAAGGGAAGAAACTTTTAGGAAACTTGCTGCTGAAGAAAAAGAAAAGCACTTGGAAGCTATTAAGGAGCTTGAGGAGGCAAAAGACTTGCTGGCTAAAGAAGCTTATGAAAGGCAGCTGGCTGAGCTTAATGCCTTAAAGGAATCCTTGGAGAAGCAGAAAATTGTTGACACTCTTCTGACTAATGATAGGAGGTACAGAAGATATACTACGGCCGAAATCGAAACTGCGACGAATTTCTTCGATGAAGTTAATGTGATCGGCGAGGGAGGATATGGGAAAGTCTACAAATGCAGCCTCGATCATACCCCTGTGGCGATTAAGGTTTTTCAACACGACATATTCGAGAAAAAGGATGAGTTTCTGAAAGAGGTATCATTTGTTACAAAATTTTCCAACTCTTCATGGTTCTTATCCAATTAATGATCCTCATTCTATCACCTGAACTTATATCTGCAGGTTGAAATTCTGAGCCAGATACGTCATCCACACGTGGTTTTACTGCTCGGAGCTTGTCCCGAGCGAGGTTGCTTGATCTATGAATACATGGAAAATGGAAGCTTAGATGATCACATCTTGCTCCGGAATGGAAAAACTCCCCTTCCTTGGTCTATTAGATTTAGAATAATTTTTCAAGTAGCTTCTGGACTTGCATTTTTACACAATTCAAAGCCAGAGCCTATTATTCATAGAGATCTAAAACCAGGTAATATCTTACTAGACAGAAACTATGTAAGCAAGATCTCTGATGTTGGAATGGCTAAGATCATCGGCGATATCGTGCCCGACAATGTCACGGAGTACAGAAACACCATTCTTGCTGGTACCTTACATTACATGGATCCTGAATATCAGAGAACTGGCACCCTCCGACCAAAGTCAGATACATATGCACTTGGAGTAACAATGCTCCAGTTGTTGACAGGGCGCCAGCCACATGGTCTTCTTCTGGCTGTCGAGAATTCCGTCGCAAGCGCCTCCCTTGCAGATATTCTAGATAAGTCAATTACTGACTGGCCTCTAGCCGAAGCAGAAGAACTAGCTCGACTTGCACTGAAATGCATGAAACTTAGATGCAGGGATAGGCCAGATCTTGAAACTGAAGTTCTTCCTGTCCTTAAAAGGCTTGTTGATTTTGCAGATACTTGTCAAAATGAAGAAAAAGGGTCTGCCAATCCTCCAAGCCACTACTTCTGCCCAATCCTCCAGGTGAATTCAAAACACTCACACACCATCATCATTTATTCATAAGCTTGACCATAAACCTCTAAGTTTTTATCTGGGTATTCTACAAATATTATAGATAACCGTTTCGATTTCATCCAGAAATTTATATAGAATCTTTGAAAAGGACTTTCCTTCATGAGAAAGAAAAGACTTACTGTCTTTAGGATCTGATGTCGCACTTTGCTCAATACTGTGGGAATCCACCATATTGCATAAATCGATTCCTTCATTTTGATCTTATATCATGATATAAGTAATTTTTATTGTGTTGGGCCTGTCGGCTAAAAATCTAACTAATTGATCTTTACAATACTTCTTCCATCAATGAGATCCTTTACTCACAGAATAAAGTATCTAGGCACACCTATTTCTTCATATTTCTACTTCTATGAAGTTTCTTCCTTTGCTACAGTAGATACATATCGTTAGTTTGGACAAAACAAATGTAGAAAGTCTAAGAACAAATTTTGTTCTAGTGCCCGAAAATAATAGTCCACATTTTTTTTTTTTTTTGAACACACAGGAAATAATGGAGGATCCTTATATAGCAGCGGACGGGTTTAGTTACGAGTATATAGCGATTAAAGCATGGCTCGAGGAACATGACATATCGCCCGTGACAAAGCTTAAGCTTCTGCATTCCATGTTTATTCCAAATCACACTTTGCGTTCTGCCATACAAGAGTGGAGATCAAGAGTGACATTTCCATCTTCCTAAATTGGAGATTTTGGCTTTACCTGTCTCAAGCATTCTTGTGGATTTTTTTTTTCCTCAAAAGAAAGAAACATCTTTGTTATTTTTTGTATAGTTATAAGTGAAATCTCATGAATGTATAGAATTGTTTATGTACTGTAAACTACTTCAAATCGTTTCATCTTTGTATTGTAAAGAGTAGTTTATATTCAATGAATGTATAGAAGTGGCTATGTACTGTAAACTACTTAAAATGTTCCATATTTATAAAGAGTAGTTTATTTCCTTCTTG

mRNA sequence

ATGACCTCCGTGGCGGTTGCCGTTAACGGCGTCCGCGGCGGCAAAGGCGGTGGCGGAAGCCGCCGCGCCGTCAGATGGGCCGTCGAGAATCTCTTGCCAACCGCCGATCGTTTCATTTTGGTTCACGTTATGCCCAAAATCACCTCCATTCCCACCCCAATGGGGGATCGCGTCGCTGTCTCGGAACTCGATGCAGATGTTGTAGCACTTTATGTGCACGATGTGAAAGAGAAATATGAACAAGTTTTTGTCCCTTTCAAGAAGTTATGTAGAAGAGAAAAGTTTCTTCTTCTGGTAGAAACTTTGATTTTGGAGGATGACAATCCTGCTACTGCACTTTTGAGATATGCATCTGAATCTGGGATTAAACGCTTGGTTTTGGGTTCTTGTTTTAGAACTTGCATAGCAAGGAAGCTAAAAGGGGATTCAGTACCCTCTGCTGTTCTGAGAACTGCTCCTAGTTCTCTTGATATTTATGTTATATATAGGAGAAGAGTCGTTACAAGAATGGCTAGTACTGCTCTATCTACTGAAACCGATTCGAGGCAGTGGATGCTTGGTGATACTGATTACTACAGGGGTTCAAGTGCCGTTAGTGAAAAATCATCTGGAACTTTTTCTTCTTCTTCCTTGTCGATTGTTCATCGAAGGGGCGATAGCCTCGAAGTTACTTCTACTGAGCAACCGAACTCTTTGAGTACTCTGACTGAGGAGGAAGATATGCGGGCTGAAGTTGAATCACTGCAACTAGAATTAGAAACCACTGTTTCCTTGTACAAACAAGCTTGTGAAGAACTAGTACATGCTCAGAAGAAGGTTCAGTCACTTACATCAGAATGCCTTGAAGAATCAAGAAGAGTAAATGCTGCCTTGGAAAGGGAAGAAACTTTTAGGAAACTTGCTGCTGAAGAAAAAGAAAAGCACTTGGAAGCTATTAAGGAGCTTGAGGAGGCAAAAGACTTGCTGGCTAAAGAAGCTTATGAAAGGCAGCTGGCTGAGCTTAATGCCTTAAAGGAATCCTTGGAGAAGCAGAAAATTGTTGACACTCTTCTGACTAATGATAGGAGGTACAGAAGATATACTACGGCCGAAATCGAAACTGCGACGAATTTCTTCGATGAAGTTAATGTGATCGGCGAGGGAGGATATGGGAAAGTCTACAAATGCAGCCTCGATCATACCCCTGTGGCGATTAAGGTTTTTCAACACGACATATTCGAGAAAAAGGATGAGTTTCTGAAAGAGGTTGAAATTCTGAGCCAGATACGTCATCCACACGTGGTTTTACTGCTCGGAGCTTGTCCCGAGCGAGGTTGCTTGATCTATGAATACATGGAAAATGGAAGCTTAGATGATCACATCTTGCTCCGGAATGGAAAAACTCCCCTTCCTTGGTCTATTAGATTTAGAATAATTTTTCAAGTAGCTTCTGGACTTGCATTTTTACACAATTCAAAGCCAGAGCCTATTATTCATAGAGATCTAAAACCAGGTAATATCTTACTAGACAGAAACTATGTAAGCAAGATCTCTGATGTTGGAATGGCTAAGATCATCGGCGATATCGTGCCCGACAATGTCACGGAGTACAGAAACACCATTCTTGCTGGTACCTTACATTACATGGATCCTGAATATCAGAGAACTGGCACCCTCCGACCAAAGTCAGATACATATGCACTTGGAGTAACAATGCTCCAGTTGTTGACAGGGCGCCAGCCACATGGTCTTCTTCTGGCTGTCGAGAATTCCGTCGCAAGCGCCTCCCTTGCAGATATTCTAGATAAGTCAATTACTGACTGGCCTCTAGCCGAAGCAGAAGAACTAGCTCGACTTGCACTGAAATGCATGAAACTTAGATGCAGGGATAGGCCAGATCTTGAAACTGAAGTTCTTCCTGTCCTTAAAAGGCTTGTTGATTTTGCAGATACTTGTCAAAATGAAGAAAAAGGGTCTGCCAATCCTCCAAGCCACTACTTCTGCCCAATCCTCCAGGAAATAATGGAGGATCCTTATATAGCAGCGGACGGGTTTAGTTACGAGTATATAGCGATTAAAGCATGGCTCGAGGAACATGACATATCGCCCGTGACAAAGCTTAAGCTTCTGCATTCCATGTTTATTCCAAATCACACTTTGCGTTCTGCCATACAAGAGTGGAGATCAAGAGTGACATTTCCATCTTCCTAA

Coding sequence (CDS)

ATGACCTCCGTGGCGGTTGCCGTTAACGGCGTCCGCGGCGGCAAAGGCGGTGGCGGAAGCCGCCGCGCCGTCAGATGGGCCGTCGAGAATCTCTTGCCAACCGCCGATCGTTTCATTTTGGTTCACGTTATGCCCAAAATCACCTCCATTCCCACCCCAATGGGGGATCGCGTCGCTGTCTCGGAACTCGATGCAGATGTTGTAGCACTTTATGTGCACGATGTGAAAGAGAAATATGAACAAGTTTTTGTCCCTTTCAAGAAGTTATGTAGAAGAGAAAAGTTTCTTCTTCTGGTAGAAACTTTGATTTTGGAGGATGACAATCCTGCTACTGCACTTTTGAGATATGCATCTGAATCTGGGATTAAACGCTTGGTTTTGGGTTCTTGTTTTAGAACTTGCATAGCAAGGAAGCTAAAAGGGGATTCAGTACCCTCTGCTGTTCTGAGAACTGCTCCTAGTTCTCTTGATATTTATGTTATATATAGGAGAAGAGTCGTTACAAGAATGGCTAGTACTGCTCTATCTACTGAAACCGATTCGAGGCAGTGGATGCTTGGTGATACTGATTACTACAGGGGTTCAAGTGCCGTTAGTGAAAAATCATCTGGAACTTTTTCTTCTTCTTCCTTGTCGATTGTTCATCGAAGGGGCGATAGCCTCGAAGTTACTTCTACTGAGCAACCGAACTCTTTGAGTACTCTGACTGAGGAGGAAGATATGCGGGCTGAAGTTGAATCACTGCAACTAGAATTAGAAACCACTGTTTCCTTGTACAAACAAGCTTGTGAAGAACTAGTACATGCTCAGAAGAAGGTTCAGTCACTTACATCAGAATGCCTTGAAGAATCAAGAAGAGTAAATGCTGCCTTGGAAAGGGAAGAAACTTTTAGGAAACTTGCTGCTGAAGAAAAAGAAAAGCACTTGGAAGCTATTAAGGAGCTTGAGGAGGCAAAAGACTTGCTGGCTAAAGAAGCTTATGAAAGGCAGCTGGCTGAGCTTAATGCCTTAAAGGAATCCTTGGAGAAGCAGAAAATTGTTGACACTCTTCTGACTAATGATAGGAGGTACAGAAGATATACTACGGCCGAAATCGAAACTGCGACGAATTTCTTCGATGAAGTTAATGTGATCGGCGAGGGAGGATATGGGAAAGTCTACAAATGCAGCCTCGATCATACCCCTGTGGCGATTAAGGTTTTTCAACACGACATATTCGAGAAAAAGGATGAGTTTCTGAAAGAGGTTGAAATTCTGAGCCAGATACGTCATCCACACGTGGTTTTACTGCTCGGAGCTTGTCCCGAGCGAGGTTGCTTGATCTATGAATACATGGAAAATGGAAGCTTAGATGATCACATCTTGCTCCGGAATGGAAAAACTCCCCTTCCTTGGTCTATTAGATTTAGAATAATTTTTCAAGTAGCTTCTGGACTTGCATTTTTACACAATTCAAAGCCAGAGCCTATTATTCATAGAGATCTAAAACCAGGTAATATCTTACTAGACAGAAACTATGTAAGCAAGATCTCTGATGTTGGAATGGCTAAGATCATCGGCGATATCGTGCCCGACAATGTCACGGAGTACAGAAACACCATTCTTGCTGGTACCTTACATTACATGGATCCTGAATATCAGAGAACTGGCACCCTCCGACCAAAGTCAGATACATATGCACTTGGAGTAACAATGCTCCAGTTGTTGACAGGGCGCCAGCCACATGGTCTTCTTCTGGCTGTCGAGAATTCCGTCGCAAGCGCCTCCCTTGCAGATATTCTAGATAAGTCAATTACTGACTGGCCTCTAGCCGAAGCAGAAGAACTAGCTCGACTTGCACTGAAATGCATGAAACTTAGATGCAGGGATAGGCCAGATCTTGAAACTGAAGTTCTTCCTGTCCTTAAAAGGCTTGTTGATTTTGCAGATACTTGTCAAAATGAAGAAAAAGGGTCTGCCAATCCTCCAAGCCACTACTTCTGCCCAATCCTCCAGGAAATAATGGAGGATCCTTATATAGCAGCGGACGGGTTTAGTTACGAGTATATAGCGATTAAAGCATGGCTCGAGGAACATGACATATCGCCCGTGACAAAGCTTAAGCTTCTGCATTCCATGTTTATTCCAAATCACACTTTGCGTTCTGCCATACAAGAGTGGAGATCAAGAGTGACATTTCCATCTTCCTAA

Protein sequence

MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAVSELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASESGIKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVTRMASTALSTETDSRQWMLGDTDYYRGSSAVSEKSSGTFSSSSLSIVHRRGDSLEVTSTEQPNSLSTLTEEEDMRAEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREETFRKLAAEEKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRYTTAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPVAIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKTPLPWSIRFRIIFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLHYMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPHGLLLAVENSVASASLADILDKSITDWPLAEAEELARLALKCMKLRCRDRPDLETEVLPVLKRLVDFADTCQNEEKGSANPPSHYFCPILQEIMEDPYIAADGFSYEYIAIKAWLEEHDISPVTKLKLLHSMFIPNHTLRSAIQEWRSRVTFPSS
Homology
BLAST of Spg003573 vs. NCBI nr
Match: XP_038891123.1 (U-box domain-containing protein 34-like [Benincasa hispida])

HSP 1 Score: 1323.1 bits (3423), Expect = 0.0e+00
Identity = 671/729 (92.04%), Postives = 703/729 (96.43%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           M SVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGD VAV
Sbjct: 1   MISVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQVFVPFKKLCRREKFLLLVETL+LEDDNPATALLRYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQVFVPFKKLCRREKFLLLVETLVLEDDNPATALLRYASES 120

Query: 121 GIKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVTRMASTALSTETD 180
           GIKRLVLGSCFRTC+ARKLKGDSVPSA++RT  SS DI+VIY+RRV+TR ASTA S ETD
Sbjct: 121 GIKRLVLGSCFRTCVARKLKGDSVPSAIMRTVHSSFDIHVIYKRRVITRKASTAPSNETD 180

Query: 181 SRQWMLGDTDYYRGSSAVSEKSSGTFSSSSLSIVHRRGDSLEVTSTEQPNSLSTLTEEED 240
           SRQWMLGDTDYYRGSSA SEKS GTFSSSSLSI HRRGDSL+V STE+ NSL+TLTEEED
Sbjct: 181 SRQWMLGDTDYYRGSSAASEKSLGTFSSSSLSIGHRRGDSLKVNSTEELNSLNTLTEEED 240

Query: 241 MRAEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREETFRKL 300
           M+AEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALERE+  RK 
Sbjct: 241 MQAEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREQALRKT 300

Query: 301 AAEEKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRY 360
            A+EKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKES+EKQKIVDTLLTNDRRYRRY
Sbjct: 301 VAKEKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKESVEKQKIVDTLLTNDRRYRRY 360

Query: 361 TTAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPVAIKVFQHDIFEKKDEFLKEVEILS 420
           TTAEIE ATNFFDEVNVIGEGGYGKVY CSLDHTPVAIKVFQHDIF+KKDEFLKEVEILS
Sbjct: 361 TTAEIEAATNFFDEVNVIGEGGYGKVYNCSLDHTPVAIKVFQHDIFKKKDEFLKEVEILS 420

Query: 421 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKTPLPWSIRFRIIFQVASGLA 480
           QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGK PLPWS RFRI+FQVASGLA
Sbjct: 421 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGLA 480

Query: 481 FLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 540
           FLHNSKPEPIIHRDLKPGNILLDRN+VSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH
Sbjct: 481 FLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 540

Query: 541 YMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPHGLLLAVENSVASASLADILDKSITD 600
           YMDPEYQRTGTLRPKSDTYALGVT+LQLLTGRQPHGLLLA+ENS+ASASLADILDKSI+D
Sbjct: 541 YMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSISD 600

Query: 601 WPLAEAEELARLALKCMKLRCRDRPDLETEVLPVLKRLVDFADTCQNEEKGSANPPSHYF 660
           WPLA+AEELARLALKC+KLRCRDRPDLE+EVLPVLKRLVDFADTC+NE+KG ANP SHYF
Sbjct: 601 WPLAKAEELARLALKCLKLRCRDRPDLESEVLPVLKRLVDFADTCENEDKGFANPLSHYF 660

Query: 661 CPILQEIMEDPYIAADGFSYEYIAIKAWLEEHDISPVTKLKLLHSMFIPNHTLRSAIQEW 720
           CPILQEIMEDPYIAADGF+YEY+AIKAWLE++DISPVTKLKL HS+FIPNHTLRSAIQEW
Sbjct: 661 CPILQEIMEDPYIAADGFTYEYVAIKAWLEKYDISPVTKLKLQHSLFIPNHTLRSAIQEW 720

Query: 721 RSRVTFPSS 730
           RSRVT  SS
Sbjct: 721 RSRVTVSSS 729

BLAST of Spg003573 vs. NCBI nr
Match: KAG6599517.1 (U-box domain-containing protein 34, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1302.7 bits (3370), Expect = 0.0e+00
Identity = 664/729 (91.08%), Postives = 697/729 (95.61%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVA+NGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGD VAV
Sbjct: 1   MTSVAVAINGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQ+FVPFKK+CRREKFLLLVETLILEDDNPATAL+RYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQIFVPFKKMCRREKFLLLVETLILEDDNPATALMRYASES 120

Query: 121 GIKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVTRMASTALSTETD 180
           GIK LVLGSCFRTCIAR+LKGDSVP A++R A    DIYVIYRRRV+TR A+TA S+ETD
Sbjct: 121 GIKHLVLGSCFRTCIARRLKGDSVPFAIMRAASRCFDIYVIYRRRVITRTATTAPSSETD 180

Query: 181 SRQWMLGDTDYYRGSSAVSEKSSGTFSSSSLSIVHRRGDSLEVTSTEQPNSLSTLTEEED 240
           SRQWMLGDTDYYRGSS VSEKS GT SSSS+S VHRRGDSLEV STEQ NSLSTLTEEED
Sbjct: 181 SRQWMLGDTDYYRGSSTVSEKSFGTLSSSSMSNVHRRGDSLEVNSTEQLNSLSTLTEEED 240

Query: 241 MRAEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREETFRKL 300
           M+AEVESLQLELE+TVSLYKQACEELVHAQKKVQSLTSECLEESR+VNAALERE+  RK+
Sbjct: 241 MQAEVESLQLELESTVSLYKQACEELVHAQKKVQSLTSECLEESRKVNAALEREQALRKV 300

Query: 301 AAEEKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRY 360
           AAEEK KHLEAIKELEEAKD LAKEAYERQLAELNALKESLEK+KIVDTLLTNDRRYRRY
Sbjct: 301 AAEEKAKHLEAIKELEEAKDSLAKEAYERQLAELNALKESLEKKKIVDTLLTNDRRYRRY 360

Query: 361 TTAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPVAIKVFQHDIFEKKDEFLKEVEILS 420
           TTAEIE ATNFFDEVNVIGEGGYGKVY CSLDHTPVAIKVF+HDIFE KDEFLKEVEILS
Sbjct: 361 TTAEIEAATNFFDEVNVIGEGGYGKVYVCSLDHTPVAIKVFRHDIFENKDEFLKEVEILS 420

Query: 421 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKTPLPWSIRFRIIFQVASGLA 480
           QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGK PLPWSIRFRI+FQVASGLA
Sbjct: 421 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKPPLPWSIRFRIVFQVASGLA 480

Query: 481 FLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 540
           FLHNSKPEPIIHRDLKPGNILLDRN+VSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH
Sbjct: 481 FLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 540

Query: 541 YMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPHGLLLAVENSVASASLADILDKSITD 600
           YMDPEYQRTGTLRPKSDTYALGVT+LQLLTGRQPHGLLLAVENS+ASASLADILDKSITD
Sbjct: 541 YMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAVENSIASASLADILDKSITD 600

Query: 601 WPLAEAEELARLALKCMKLRCRDRPDLETEVLPVLKRLVDFADTCQNEEKGSANPPSHYF 660
           WP+AEAEELA LALKC+KLRCRDRPDLETEVLPVLKRLVDFADT Q   KG+ANPPSHYF
Sbjct: 601 WPVAEAEELAGLALKCLKLRCRDRPDLETEVLPVLKRLVDFADTFQ---KGAANPPSHYF 660

Query: 661 CPILQEIMEDPYIAADGFSYEYIAIKAWLEEHDISPVTKLKLLHSMFIPNHTLRSAIQEW 720
           CPILQE+MEDPYIAADGF+YEY AIKAWLE++D+SPVTKLKL HSMFIPN+TLRSAIQEW
Sbjct: 661 CPILQEVMEDPYIAADGFTYEYEAIKAWLEKYDVSPVTKLKLQHSMFIPNYTLRSAIQEW 720

Query: 721 RSRVTFPSS 730
           RSR+TF SS
Sbjct: 721 RSRLTFSSS 726

BLAST of Spg003573 vs. NCBI nr
Match: XP_023546893.1 (U-box domain-containing protein 34-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1300.4 bits (3364), Expect = 0.0e+00
Identity = 662/729 (90.81%), Postives = 697/729 (95.61%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVA+NGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGD VAV
Sbjct: 21  MTSVAVAINGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 80

Query: 61  SELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQ+FVPFKK+CRREKFLLLVETLILEDDNPATAL+RYASES
Sbjct: 81  SELDADVVALYVHDVKQKYEQIFVPFKKMCRREKFLLLVETLILEDDNPATALVRYASES 140

Query: 121 GIKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVTRMASTALSTETD 180
           GIK LVLGSCFRTCIAR+LKGDSVPSA++R A    DIYVIYRRRV+TR A+TA S+ETD
Sbjct: 141 GIKHLVLGSCFRTCIARRLKGDSVPSAIMRAASRCFDIYVIYRRRVITRTATTAPSSETD 200

Query: 181 SRQWMLGDTDYYRGSSAVSEKSSGTFSSSSLSIVHRRGDSLEVTSTEQPNSLSTLTEEED 240
           SRQWMLGDTDYYRGSS VSE+S GT SSSS+S VHRRGDSLEV STEQ NSLSTLTEEED
Sbjct: 201 SRQWMLGDTDYYRGSSTVSERSFGTLSSSSMSNVHRRGDSLEVNSTEQLNSLSTLTEEED 260

Query: 241 MRAEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREETFRKL 300
           M+AEVESLQLELE+TVSLYKQACEELVHAQKKVQSLTSECLEESR+VNAALERE+  RK+
Sbjct: 261 MQAEVESLQLELESTVSLYKQACEELVHAQKKVQSLTSECLEESRKVNAALEREQALRKV 320

Query: 301 AAEEKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRY 360
           AAEEK KHLEAIKELEEAKD LAKEAYERQLAELNALKESLEK+KIVDTLLTNDRRYRRY
Sbjct: 321 AAEEKAKHLEAIKELEEAKDSLAKEAYERQLAELNALKESLEKKKIVDTLLTNDRRYRRY 380

Query: 361 TTAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPVAIKVFQHDIFEKKDEFLKEVEILS 420
           TTAEIE ATNFFDEVNVIGEGGYGKVY CSLDHTPVAIKVF+HDIFE KDEFLKEVEILS
Sbjct: 381 TTAEIEAATNFFDEVNVIGEGGYGKVYVCSLDHTPVAIKVFRHDIFENKDEFLKEVEILS 440

Query: 421 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKTPLPWSIRFRIIFQVASGLA 480
           QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGK PLPWSIRFRI+FQVASGLA
Sbjct: 441 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKPPLPWSIRFRIVFQVASGLA 500

Query: 481 FLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 540
           FLHNSKPEPIIHRDLKPGNILLDRN+VSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH
Sbjct: 501 FLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 560

Query: 541 YMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPHGLLLAVENSVASASLADILDKSITD 600
           YMDPEYQRTGTLRPKSDTYALGVT+LQLLTGRQPHGLLLAVENS+AS SLADILDKSITD
Sbjct: 561 YMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAVENSIASTSLADILDKSITD 620

Query: 601 WPLAEAEELARLALKCMKLRCRDRPDLETEVLPVLKRLVDFADTCQNEEKGSANPPSHYF 660
           WP+AEAEELA LALKC+KLRCRDRPDLETEVLPVLKRLVDFADT Q   KG+ANPPSHYF
Sbjct: 621 WPVAEAEELAGLALKCLKLRCRDRPDLETEVLPVLKRLVDFADTFQ---KGAANPPSHYF 680

Query: 661 CPILQEIMEDPYIAADGFSYEYIAIKAWLEEHDISPVTKLKLLHSMFIPNHTLRSAIQEW 720
           CPILQE+MEDPYIAADGF+YE+ AIKAWLE++D+SPVTKLKL HSMFIPN+TLRSAIQEW
Sbjct: 681 CPILQEVMEDPYIAADGFTYEFEAIKAWLEKYDVSPVTKLKLQHSMFIPNYTLRSAIQEW 740

Query: 721 RSRVTFPSS 730
           RSR+TF SS
Sbjct: 741 RSRLTFSSS 746

BLAST of Spg003573 vs. NCBI nr
Match: XP_022946754.1 (U-box domain-containing protein 34-like [Cucurbita moschata])

HSP 1 Score: 1299.3 bits (3361), Expect = 0.0e+00
Identity = 661/729 (90.67%), Postives = 696/729 (95.47%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVA+NGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGD VAV
Sbjct: 1   MTSVAVAINGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQ+FVPFKK+CRREKFLLLVETLILEDDNPATAL+RYA+ES
Sbjct: 61  SELDADVVALYVHDVKQKYEQIFVPFKKMCRREKFLLLVETLILEDDNPATALMRYATES 120

Query: 121 GIKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVTRMASTALSTETD 180
           GIK LVLGSCFRTCIAR+LKGDSVPSA++R A    D+YVIYRRRV+TR A+TA S+ETD
Sbjct: 121 GIKHLVLGSCFRTCIARRLKGDSVPSAIMRAASRCFDVYVIYRRRVITRTATTAPSSETD 180

Query: 181 SRQWMLGDTDYYRGSSAVSEKSSGTFSSSSLSIVHRRGDSLEVTSTEQPNSLSTLTEEED 240
           SRQWMLGDTDYYRGSS VSEKS GT SSSS+S VHRRGDSLEV STEQ NSLSTLTEEED
Sbjct: 181 SRQWMLGDTDYYRGSSTVSEKSFGTLSSSSMSNVHRRGDSLEVNSTEQLNSLSTLTEEED 240

Query: 241 MRAEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREETFRKL 300
           M+AEVESLQLELE+TVSLYKQACEELVHAQKKVQSLTSECLEESR+VNAALERE+  RK+
Sbjct: 241 MQAEVESLQLELESTVSLYKQACEELVHAQKKVQSLTSECLEESRKVNAALEREQALRKV 300

Query: 301 AAEEKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRY 360
           AAEEK KHLEAIKELEEAKD LAKEAYERQLAELNALKESLEK+KIVDTLLTNDRRYRRY
Sbjct: 301 AAEEKAKHLEAIKELEEAKDSLAKEAYERQLAELNALKESLEKKKIVDTLLTNDRRYRRY 360

Query: 361 TTAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPVAIKVFQHDIFEKKDEFLKEVEILS 420
           TTAEIE ATNFFDEVNVIGEGGYGKVY CSLDHTPVAIKVF+HDIFE KDEFLKEVEILS
Sbjct: 361 TTAEIEAATNFFDEVNVIGEGGYGKVYVCSLDHTPVAIKVFRHDIFENKDEFLKEVEILS 420

Query: 421 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKTPLPWSIRFRIIFQVASGLA 480
           QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGK PLPWSIRFRI+FQVASGLA
Sbjct: 421 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKPPLPWSIRFRIVFQVASGLA 480

Query: 481 FLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 540
            LHNSKPEPIIHRDLKPGNILLDRN+VSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH
Sbjct: 481 LLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 540

Query: 541 YMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPHGLLLAVENSVASASLADILDKSITD 600
           YMDPEYQRTGTLRP SDTYALGVT+LQLLTGRQPHGLLLAVENS+ASASLADILDKSITD
Sbjct: 541 YMDPEYQRTGTLRPNSDTYALGVTILQLLTGRQPHGLLLAVENSIASASLADILDKSITD 600

Query: 601 WPLAEAEELARLALKCMKLRCRDRPDLETEVLPVLKRLVDFADTCQNEEKGSANPPSHYF 660
           WP+AEAEELA LALKC+KLRCRDRPDLETEVLPVLKRLVDFADT Q   KG+ANPPSHYF
Sbjct: 601 WPVAEAEELAGLALKCLKLRCRDRPDLETEVLPVLKRLVDFADTFQ---KGAANPPSHYF 660

Query: 661 CPILQEIMEDPYIAADGFSYEYIAIKAWLEEHDISPVTKLKLLHSMFIPNHTLRSAIQEW 720
           CPILQE+MEDPYIAADGF+YEY AIKAWLE++D+SPVTKLKL HSMFIPN+TLRSAIQEW
Sbjct: 661 CPILQEVMEDPYIAADGFTYEYEAIKAWLEKYDVSPVTKLKLQHSMFIPNYTLRSAIQEW 720

Query: 721 RSRVTFPSS 730
           RSR+TF SS
Sbjct: 721 RSRLTFSSS 726

BLAST of Spg003573 vs. NCBI nr
Match: XP_022999433.1 (U-box domain-containing protein 34-like [Cucurbita maxima])

HSP 1 Score: 1298.9 bits (3360), Expect = 0.0e+00
Identity = 662/729 (90.81%), Postives = 695/729 (95.34%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVA+NGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGD VAV
Sbjct: 1   MTSVAVAINGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQ+FVPFKK+CRREKFLLLVETLILEDDNPATAL+RYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQIFVPFKKMCRREKFLLLVETLILEDDNPATALMRYASES 120

Query: 121 GIKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVTRMASTALSTETD 180
           GIK LVLGSCFRTCIAR+LKGDSVPSA++R A    DIYVIYRRRV+TR A+TA S+ETD
Sbjct: 121 GIKHLVLGSCFRTCIARRLKGDSVPSAIMRAASRCFDIYVIYRRRVITRTATTAPSSETD 180

Query: 181 SRQWMLGDTDYYRGSSAVSEKSSGTFSSSSLSIVHRRGDSLEVTSTEQPNSLSTLTEEED 240
           SRQWMLGDTDYYRGSS VSEKS GT SSSS+S VHRRGDSLEV STEQ NSLSTLTEEED
Sbjct: 181 SRQWMLGDTDYYRGSSTVSEKSFGTLSSSSMSNVHRRGDSLEVNSTEQLNSLSTLTEEED 240

Query: 241 MRAEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREETFRKL 300
           M+AEVESLQLELE+TVSLYKQACEELVHAQKKVQSLTSECLEESR+VNAALERE+  RK+
Sbjct: 241 MQAEVESLQLELESTVSLYKQACEELVHAQKKVQSLTSECLEESRKVNAALEREQALRKV 300

Query: 301 AAEEKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRY 360
           AAEEK KHLEAIKELEEAKD LAKEAYERQLAELNALKESLEK+KIVDTLLTNDRRYRRY
Sbjct: 301 AAEEKAKHLEAIKELEEAKDSLAKEAYERQLAELNALKESLEKKKIVDTLLTNDRRYRRY 360

Query: 361 TTAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPVAIKVFQHDIFEKKDEFLKEVEILS 420
           TTAEIE ATNFFDEVNVIGEGGYGKVY CSLDHTPVAIKVF+HDIFE KDEFLKEVEILS
Sbjct: 361 TTAEIEAATNFFDEVNVIGEGGYGKVYVCSLDHTPVAIKVFRHDIFENKDEFLKEVEILS 420

Query: 421 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKTPLPWSIRFRIIFQVASGLA 480
           QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGK PLPWSIR RI+FQVASGLA
Sbjct: 421 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKPPLPWSIRLRIVFQVASGLA 480

Query: 481 FLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 540
           FLHNSKPEPIIHRDLKPGNILLDRN+VSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH
Sbjct: 481 FLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 540

Query: 541 YMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPHGLLLAVENSVASASLADILDKSITD 600
           YMDPEYQRTGTLRPKSDTYALGVT+LQLLTGRQPHGLLLAVENS+AS SLADILDKSITD
Sbjct: 541 YMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAVENSIASGSLADILDKSITD 600

Query: 601 WPLAEAEELARLALKCMKLRCRDRPDLETEVLPVLKRLVDFADTCQNEEKGSANPPSHYF 660
           WPLAEAEELA LALKC+KLRCRDRPDLETEVLPVLKRLVDFAD  +   KG+ANPPSHYF
Sbjct: 601 WPLAEAEELAGLALKCLKLRCRDRPDLETEVLPVLKRLVDFADAFR---KGAANPPSHYF 660

Query: 661 CPILQEIMEDPYIAADGFSYEYIAIKAWLEEHDISPVTKLKLLHSMFIPNHTLRSAIQEW 720
           CPILQE+MEDPYIAADGF+YEY AIKAWLE++D+SPVTKLKL HSMFIPN+TLRSAIQEW
Sbjct: 661 CPILQEVMEDPYIAADGFTYEYEAIKAWLEKYDVSPVTKLKLQHSMFIPNYTLRSAIQEW 720

Query: 721 RSRVTFPSS 730
           RSR+TF SS
Sbjct: 721 RSRLTFSSS 726

BLAST of Spg003573 vs. ExPASy Swiss-Prot
Match: Q8S8S7 (U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 1.7e-211
Identity = 409/807 (50.68%), Postives = 528/807 (65.43%), Query Frame = 0

Query: 4   VAVAVNGVRGGK-GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTP--------- 63
           VAVAV G+ G K GG GSRRAVRWAV+NLLP AD+F+++HV+P ITSIPTP         
Sbjct: 24  VAVAVKGLIGDKLGGAGSRRAVRWAVDNLLPKADKFVMIHVIPTITSIPTPNILILMFTR 83

Query: 64  -----MGDRVAVSELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDN 123
                 GDR+ V E++  VV +YV DVK++YE VFVPF K+C+                 
Sbjct: 84  MWVVTAGDRLPVEEVEESVVEMYVRDVKKEYETVFVPFLKMCK----------------- 143

Query: 124 PATALLRYASESGIKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVT 183
                    S    KR      FR   +R+ KG  VP  VLR AP + ++Y++ + R+ T
Sbjct: 144 ---------STRSTKRY-----FR---SRRTKGTGVPLTVLRYAPETCEVYIVCKDRITT 203

Query: 184 RMASTALSTETDS------------RQWMLG----------------DTDYYRGSSA--- 243
           +     ++ E  +            R W                   +    R +SA   
Sbjct: 204 KSMDPLINREPCTSPHAAATAHDFLRDWAASFHTLRSPTLPDPRQSTEAGTRRSASAREL 263

Query: 244 ------------VSEKSSGTFSSSSLSIVHRRGDS---------LEVTSTEQPNSLSTLT 303
                        + +SS   S+++  I  RR  S          + T T+  +++  + 
Sbjct: 264 RFEALSLTCNKPKTPQSSKASSATTPEIFRRRRGSDIPQLNYSDFDKTCTKPQSNVENIV 323

Query: 304 EEE-----------------DMRAEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSE 363
            E                  ++  EVE L+ EL++TV  YKQACEEL   Q KV+ L++E
Sbjct: 324 SEHRDSDRSPPETSRKSKKVEIEEEVERLKNELQSTVFKYKQACEELFSTQNKVKMLSTE 383

Query: 364 CLEESRRVNAALEREETFRKLAAEEKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKE 423
            L ES+RVN A+E+EE  R  AA EKE++++A+KE+E AK LLA+E  +RQ+AE+NAL+ 
Sbjct: 384 YLNESKRVNNAVEKEELQRNTAALEKERYMKAVKEVETAKALLAREFCQRQIAEVNALRT 443

Query: 424 SLEKQKIVDTLLTNDRRYRRYTTAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPVAIK 483
            LEK+K++D LL  D RYR+YT  EI TAT  F    VIGEGGYGKVY+CSLD TP A+K
Sbjct: 444 YLEKKKVIDQLLGTDHRYRKYTIEEIVTATEGFSPEKVIGEGGYGKVYQCSLDSTPAAVK 503

Query: 484 VFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNG 543
           V + D  EKK EFLKEVE+LSQ+RHPHVVLLLGACPE GCL+YEY+ENGSL+++I  R  
Sbjct: 504 VVRLDTPEKKQEFLKEVEVLSQLRHPHVVLLLGACPENGCLVYEYLENGSLEEYIFHRKN 563

Query: 544 KTPLPWSIRFRIIFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMAKII 603
           K PLPW IRFR+IF+VA GLAFLH+SKPEPI+HRDLKPGNILL+RNYVSKI+DVG+AK++
Sbjct: 564 KPPLPWFIRFRVIFEVACGLAFLHSSKPEPIVHRDLKPGNILLNRNYVSKIADVGLAKLV 623

Query: 604 GDIVPDNVTEYRNTILAGTLHYMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPHGLLL 663
            D+ PDNVT YRN++LAGTLHY+DPEY RTGT+RPKSD YA G+ +LQLLT R P G++ 
Sbjct: 624 TDVAPDNVTMYRNSVLAGTLHYIDPEYHRTGTIRPKSDLYAFGIIILQLLTARNPSGIVP 683

Query: 664 AVENSVASASLADILDKSITDWPLAEAEELARLALKCMKLRCRDRPDLETEVLPVLKRLV 723
           AVEN+V   +L ++LDKS+TDWPLAE EELAR+ LKC + RCRDRPDL++EV+PVLKRLV
Sbjct: 684 AVENAVKKGTLTEMLDKSVTDWPLAETEELARIGLKCAEFRCRDRPDLKSEVIPVLKRLV 743

Query: 724 DFADTCQNEEKGSANPPSHYFCPILQEIMEDPYIAADGFSYEYIAIKAWLEEHDISPVTK 727
           + A++   +E  +   PSHYFCPIL+EIME+P IAADGF+YE  AI AWLE+H+ISPVT+
Sbjct: 744 ETANSKVKKEGSNLRAPSHYFCPILREIMEEPEIAADGFTYERKAILAWLEKHNISPVTR 796

BLAST of Spg003573 vs. ExPASy Swiss-Prot
Match: Q9FKG6 (U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 3.1e-136
Identity = 315/831 (37.91%), Postives = 450/831 (54.15%), Query Frame = 0

Query: 3   SVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTA-DRFILVHVMPKITSIPTPMGDRVAVS 62
           SVAVA+NG +       S+  V WA+E  +P     F L++V P ++ IPTPMG  VAVS
Sbjct: 23  SVAVAINGKK------KSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVS 82

Query: 63  ELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASESG 122
           EL  DVV+ Y  ++     ++  P+KK+  R K  + VE L+L+   PA A+    + +G
Sbjct: 83  ELREDVVSAYKQELDWSANEMLRPYKKMFERRK--VQVEVLLLDSLEPAAAIAEEIAGTG 142

Query: 123 IKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVT------------- 182
           + +LV+G   R   +RK+    + S +    P    +YVI + ++ +             
Sbjct: 143 VTKLVIGMSLRGFFSRKI---DMSSLIATAVPRFCTVYVISKGKLASVRPSESDASGSIR 202

Query: 183 --RMASTALST-----------------ETDSRQWMLGDT-DYYRGSSAVSE---KSSGT 242
             R +ST+ ST                 E  SR         +  GS+AV++    SSGT
Sbjct: 203 FERSSSTSGSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSSGT 262

Query: 243 -----FSSSSLSIVH-------RRGDSLEVT---STEQPNSLSTLT---EEEDMRA---- 302
                 +   + IVH        + +S   +    TE  NS+S  +   + ED R     
Sbjct: 263 DQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRREMRSS 322

Query: 303 --------------------------------------------------EVESLQLELE 362
                                                             E+E L+ EL+
Sbjct: 323 SSSNNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRAELK 382

Query: 363 TTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREETFRKLAAEEKEKHLEAIK 422
               +Y  A  E V A KK+  L     EES ++    E+EE  +  A++EK+++ EA+K
Sbjct: 383 HVQEMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEKEEVAKDTASKEKQRYEEAMK 442

Query: 423 ELEEAKDLLAKEAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRYTTAEIETATNFFD 482
           E E+ K+L+ KEA  R+ AE  A +++ EK K+  +L++   +Y+ YT  EI  AT+ F 
Sbjct: 443 EAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQHYTWEEIAAATSDFA 502

Query: 483 EVNVIGEGGYGKVYKCSLDHTPVAIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGA 542
           E   IG G YG VYKC+L HT  A+KV      +   +F +E+EILS+IRHPH+VLLLGA
Sbjct: 503 ENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRHPHLVLLLGA 562

Query: 543 CPERGCLIYEYMENGSLDDHILLRNGKTPLPWSIRFRIIFQVASGLAFLHNSKPEPIIHR 602
           CPERGCL+YEYM+NGSLDD ++L N   P+PW  RFRI  +VAS L FLH SKP PIIHR
Sbjct: 563 CPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASALVFLHKSKPRPIIHR 622

Query: 603 DLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLHYMDPEYQRTGTLR 662
           DLKPGNILLD N+VSK+ DVG++ ++      + T ++ T   GTL Y+DPEYQRTG + 
Sbjct: 623 DLKPGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGTLCYIDPEYQRTGIIS 682

Query: 663 PKSDTYALGVTMLQLLTGRQPHGLLLAVENSVA-SASLADILDKSITDWPLAEAEELARL 722
           PKSD Y+LGV +LQL+T +    +   VE ++   A    ILDK    WP+++  ELA L
Sbjct: 683 PKSDVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKKAGSWPISDTRELAAL 742

BLAST of Spg003573 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 455.3 bits (1170), Expect = 1.3e-126
Identity = 285/809 (35.23%), Postives = 423/809 (52.29%), Query Frame = 0

Query: 16  GGGGSRRAVRWAVENLLPTAD-RFILVHVMPKITSIPTPMGDRVAVSELDADVVALYVHD 75
           G   S+  V WA+E      +  F L+H+ P ITS+PTPMG+ + +SE+  DVV  Y  +
Sbjct: 28  GSSKSKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISEVRDDVVTAYRQE 87

Query: 76  VKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASESGIKRLVLGSCFRTC 135
           +  + E++  P+ KL  R K  + VE L++E DN A A+    +   I R+V+G   R+ 
Sbjct: 88  ILWQSEEMLKPYTKLFVRRK--VAVEVLVIESDNVAAAIAEEVTRDSIDRIVIGGSSRSF 147

Query: 136 IARKLKGDSVPSAVL--------------------------------------------- 195
            +RK    SV SA++                                             
Sbjct: 148 FSRKADICSVISALMPNFCTVYVVSKGKLSCVRPSDSDGNATIREDGSERTNSSSGSSGP 207

Query: 196 --------------RTAPSSLDIYVIYRRRVVTRMASTAL-------------------- 255
                         ++ P SL +  +     +   AS  +                    
Sbjct: 208 TSDSSDVMSSAHDSQSRPLSLPVRRMQHFPAIAGQASVPMETSSVGSDETRCMSLDAEEA 267

Query: 256 ---------STETDSRQWMLGDTDYYRGSSAVSEKSSG----------TFSSSSLSIVHR 315
                    ST+T SR W     DY     A+S  SS           ++S   +   H 
Sbjct: 268 RDVSSINRSSTDTTSR-WTPRRRDYEERKEAMSSSSSNREYGNFGTRFSWSGMGVDTTHS 327

Query: 316 RGDSLEVTSTEQPNSLSTLTEEEDMRAEVESLQLELETTVSLYKQACEELVHAQKKVQSL 375
           R        ++  +  S    + ++  EVE L+ EL     +Y  A  E   A +K+  L
Sbjct: 328 RASQQASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYAVAQTETFDASRKLGEL 387

Query: 376 TSECLEESRRVNAALEREETFRKLAAEEKEKHLEAIKELEEAKDLLAKEAYERQLAELNA 435
               LEE+ ++     +E   R+LA +EK+   +A ++ E  ++   +E  +R+ AE  +
Sbjct: 388 NQRRLEEAIKLEELKLKEYEARELAEKEKQNFEKARRDAESMRERAEREIAQRREAERKS 447

Query: 436 LKESLEKQKIVDTLLTNDRRYRRYTTAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPV 495
            +++ EK+K+  TL +   +Y+ +   EI  AT+ F E   IG G YG VYKC+L HT  
Sbjct: 448 ARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGMGAYGAVYKCNLHHTTA 507

Query: 496 AIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILL 555
            +KV Q    +   +F +E+EILS+IRHPH+VLLLGACPE+G L+YEYMENGSL+D +  
Sbjct: 508 VVKVLQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLFQ 567

Query: 556 RNGKTPLPWSIRFRIIFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMA 615
            N   PLPW  RFRI ++VA+ L FLH SKP+PIIHRDLKP NILLD N+VSK+ DVG++
Sbjct: 568 VNNSPPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGLS 627

Query: 616 KIIG-DIVPDNVTEYRNTILAGTLHYMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPH 675
            ++  D +    T Y+ T   GTL Y+DPEYQRTG +  KSD Y+ G+ +LQLLT +   
Sbjct: 628 TMVQVDPLSTKFTIYKQTSPVGTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAKPAI 687

Query: 676 GLLLAVENSV-ASASLADILDKSITDWPLAEAEELARLALKCMKLRCRDRPDLETEVLPV 723
            L   VE+++ ++     ILD+   +WP+ E  ELA LAL C +LR +DRPDL+ ++LP 
Sbjct: 688 ALTHFVESAMDSNDEFLKILDQKAGNWPIEETRELAALALCCTELRGKDRPDLKDQILPA 747

BLAST of Spg003573 vs. ExPASy Swiss-Prot
Match: Q9LU47 (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 2.8e-121
Identity = 291/800 (36.38%), Postives = 431/800 (53.87%), Query Frame = 0

Query: 3   SVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTAD-RFILVHVMPKITSIPTPMGDRVAVS 62
           +VA+A++      G   S+  ++WA+       +  F L+H+ PKIT++PT  G+ V++S
Sbjct: 33  TVALAIS------GSIKSKNVIKWALNKFGSDKNVTFKLIHIHPKITTLPTASGNIVSIS 92

Query: 63  ELDADVVALYVHDV-KEKYEQVFVPFKKLCRREKFLL------------LVETLILEDDN 122
           E   +V A Y   V +E  E +  PFKK+C R+K  +             VE  +LE ++
Sbjct: 93  EELEEVAAAYRQKVMQETKETLLKPFKKMCERKKLKIDETRFESSLTKVAVELQVLESNS 152

Query: 123 PATALLRYASESGIKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVT 182
            A A+ +  ++  I  L++G   R+  A   +   + +++  +  +   +YV+    V  
Sbjct: 153 VAVAITKEVNQHLISNLIIG---RSSQAASSRNYDITASISASVSNLCTVYVVSNGGVHI 212

Query: 183 RMASTALSTETDSRQWMLG----DTDYYRGSSAVSEKSSGTFSSSSLSIVHRRGDSL--- 242
            +A     TE +      G     +    GS A S+  S    S+  ++ ++R  +L   
Sbjct: 213 -LAKDTSDTERNDTSIESGFERTSSSCSSGSGANSDVMSNALKSNPHTLSNKRMQNLPTI 272

Query: 243 --------EVTSTEQPNS---LSTLTEEEDMRAEVESLQ--------------------- 302
                   E +STE   +    S   EE   R+  E+ +                     
Sbjct: 273 VRGVSVPMETSSTESDETKKRSSDAAEEASKRSSPETSRSVSWNPQFRDFDERKDAMSSM 332

Query: 303 -----------------LELETTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALE 362
                             + + T++   +   EL HA +       E L+ SR++N    
Sbjct: 333 SSNFEYGNVVTPLGHYFTDNQDTLNEISKLRAELRHAHEMYAVAQVETLDASRKLN---- 392

Query: 363 REETFRKLAAEEKEKHLEAIKELE--EAKDLLAKEAYERQLAELNALKESLEKQKIVDTL 422
            E  F +L   E E    A KE E  E K    +EA +R+ AE+ A  E+ EK+K+ ++ 
Sbjct: 393 -ELKFEELTLLEHETKGIAKKETEKFEQKRREEREAAQRREAEMKATHEAKEKEKLEESS 452

Query: 423 LTNDR-RYRRYTTAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPVAIKVFQHDIFEKK 482
           L   + +Y+ +T  EI  AT+ F E   IG G YG VYKC+L HT  A+KV         
Sbjct: 453 LVAPKLQYQEFTWEEIINATSSFSEDLKIGMGAYGDVYKCNLHHTIAAVKVLHSAESSLS 512

Query: 483 DEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKTPLPWSIRF 542
            +F +E+EILS+IRHPH+VLLLGACP+ G L+YEYMENGSL+D +   N   P+PW +R 
Sbjct: 513 KQFDQELEILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQVNDSQPIPWFVRL 572

Query: 543 RIIFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMAKII--GDIVPDNV 602
           RI ++VAS L FLH SKP PIIHRDLKP NILL+ N+VSK+ DVG++ +I   D +    
Sbjct: 573 RIAWEVASALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLSTMIQAADPLSTKF 632

Query: 603 TEYRNTILAGTLHYMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPHGLLLAVENSVAS 662
           T Y+ T   GTL Y+DPEYQRTG + PKSD YA G+ +LQLLTG+Q   L   VE ++ +
Sbjct: 633 TMYKQTSPVGTLCYIDPEYQRTGRISPKSDVYAFGMIILQLLTGQQAMALTYTVETAMEN 692

Query: 663 AS---LADILDKSITDWPLAEAEELARLALKCMKLRCRDRPDLETEVLPVLKRLVDFADT 722
            +   L  ILD+   +WP+ E  +LA LAL+C +LR +DRPDLE ++LPVL+ L   AD 
Sbjct: 693 NNDDELIQILDEKAGNWPIEETRQLAALALQCTELRSKDRPDLEDQILPVLESLKKVADK 752

Query: 723 CQNE-EKGSANPPSHYFCPILQEIMEDPYIAADGFSYEYIAIKAWLEEHDISPVTKLKLL 724
            +N      + PPSH+FCP+L+++M++P IAADG++Y+  AI+ W+E H  SPVT   L 
Sbjct: 753 ARNSLSAAPSQPPSHFFCPLLKDVMKEPCIAADGYTYDRRAIEEWMENHRTSPVTNSPLQ 812

BLAST of Spg003573 vs. ExPASy Swiss-Prot
Match: Q9FKG5 (U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2 SV=2)

HSP 1 Score: 399.8 bits (1026), Expect = 6.5e-110
Identity = 229/556 (41.19%), Postives = 335/556 (60.25%), Query Frame = 0

Query: 185 MLGDTDYYRGSSAVSEKSSGTFSSSSLSIVHRRGDSLEVTSTEQPNSLSTLTEEEDMRAE 244
           +L    +YR SS+         SS   S +     S   ++   P S S+   ++    E
Sbjct: 245 LLNQKGFYRTSSSGIGYGGSDISSWRSSQMEEASSS---STYSDPTSSSSQIHKD---FE 304

Query: 245 VESLQLELETTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREETFRKLAAEE 304
           +E L++EL     +Y  A  E++ A KK+Q L     EE+ R+     REE   ++   E
Sbjct: 305 LEKLKIELRHIKGMYAVAQSEVIDASKKMQDLNQRRSEEATRLKNLTIREEEADEVVEME 364

Query: 305 KEKHLEAIKELEEAKDLLAKEAYERQLAELNALKESLEKQKIVDTL---LTNDRRYRRYT 364
           +E+  +A  E E  ++ + +E  ER  AE  A +   EKQ++ D L       ++Y ++ 
Sbjct: 365 RERQEDAENEAELVRECIERETEERLEAEARAEEVRKEKQRLEDALEGGPLQRQQYMKFE 424

Query: 365 TAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPVAIKVFQHDIFEKKDEFLKEVEILSQ 424
             EI  AT+ F +   IG GGYG VY+C+L HT VA+KV   D      +F +E+EILS+
Sbjct: 425 WEEIVEATSSFSDELKIGVGGYGSVYRCNLHHTTVAVKVLHSDKSSLTKQFHQELEILSK 484

Query: 425 IRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLR--NGKTPLP----WSIRFRIIFQV 484
           IRHPH++LLLGACPERG L+YEYM NGSL++ ++ R  N  TP P    W  RFRI +++
Sbjct: 485 IRHPHLLLLLGACPERGSLVYEYMHNGSLEERLMKRRPNVDTPQPPPLRWFERFRIAWEI 544

Query: 485 ASGLAFLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTIL 544
           AS L FLH ++P PI+HRDLKP NILLDRN VSKI DVG++K++        T +  T  
Sbjct: 545 ASALYFLHTNEPRPIVHRDLKPANILLDRNNVSKIGDVGLSKMVNLDPSHASTVFNETGP 604

Query: 545 AGTLHYMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPHGLLLAVENSV--ASASLADI 604
            GT  Y+DPEYQRTG + P+SD YA G+ +LQL+T R   GL  ++E ++   +    +I
Sbjct: 605 VGTFFYIDPEYQRTGVVTPESDIYAFGIILLQLVTARSAMGLAHSIEKALRDQTGKFTEI 664

Query: 605 LDKSITDWPLAEAEELARLALKCMKLRCRDRPDLETEVLPVLKRLVDFADTCQNEEKGSA 664
           LDK+  DWP+ EA+E+  + L+C ++R RDRPDL  E+LPVL+RL + A   +N    + 
Sbjct: 665 LDKTAGDWPVKEAKEMVMIGLRCAEMRKRDRPDLGKEILPVLERLKEVASIARNMFADNL 724

Query: 665 -----NPPSHYFCPILQEIMEDPYIAADGFSYEYIAIKAWLEEHDISPVTKLKLLHSMFI 724
                N P+H++CPI +++ME+P +A+DG++YE  AIK WL+++  SP+T L       +
Sbjct: 725 IDHHHNAPTHFYCPITKDVMENPCVASDGYTYEKRAIKEWLQKNHKSPMTDLPFPSDSLL 784

BLAST of Spg003573 vs. ExPASy TrEMBL
Match: A0A6J1G4U5 (E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111450728 PE=4 SV=1)

HSP 1 Score: 1299.3 bits (3361), Expect = 0.0e+00
Identity = 661/729 (90.67%), Postives = 696/729 (95.47%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVA+NGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGD VAV
Sbjct: 1   MTSVAVAINGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQ+FVPFKK+CRREKFLLLVETLILEDDNPATAL+RYA+ES
Sbjct: 61  SELDADVVALYVHDVKQKYEQIFVPFKKMCRREKFLLLVETLILEDDNPATALMRYATES 120

Query: 121 GIKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVTRMASTALSTETD 180
           GIK LVLGSCFRTCIAR+LKGDSVPSA++R A    D+YVIYRRRV+TR A+TA S+ETD
Sbjct: 121 GIKHLVLGSCFRTCIARRLKGDSVPSAIMRAASRCFDVYVIYRRRVITRTATTAPSSETD 180

Query: 181 SRQWMLGDTDYYRGSSAVSEKSSGTFSSSSLSIVHRRGDSLEVTSTEQPNSLSTLTEEED 240
           SRQWMLGDTDYYRGSS VSEKS GT SSSS+S VHRRGDSLEV STEQ NSLSTLTEEED
Sbjct: 181 SRQWMLGDTDYYRGSSTVSEKSFGTLSSSSMSNVHRRGDSLEVNSTEQLNSLSTLTEEED 240

Query: 241 MRAEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREETFRKL 300
           M+AEVESLQLELE+TVSLYKQACEELVHAQKKVQSLTSECLEESR+VNAALERE+  RK+
Sbjct: 241 MQAEVESLQLELESTVSLYKQACEELVHAQKKVQSLTSECLEESRKVNAALEREQALRKV 300

Query: 301 AAEEKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRY 360
           AAEEK KHLEAIKELEEAKD LAKEAYERQLAELNALKESLEK+KIVDTLLTNDRRYRRY
Sbjct: 301 AAEEKAKHLEAIKELEEAKDSLAKEAYERQLAELNALKESLEKKKIVDTLLTNDRRYRRY 360

Query: 361 TTAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPVAIKVFQHDIFEKKDEFLKEVEILS 420
           TTAEIE ATNFFDEVNVIGEGGYGKVY CSLDHTPVAIKVF+HDIFE KDEFLKEVEILS
Sbjct: 361 TTAEIEAATNFFDEVNVIGEGGYGKVYVCSLDHTPVAIKVFRHDIFENKDEFLKEVEILS 420

Query: 421 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKTPLPWSIRFRIIFQVASGLA 480
           QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGK PLPWSIRFRI+FQVASGLA
Sbjct: 421 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKPPLPWSIRFRIVFQVASGLA 480

Query: 481 FLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 540
            LHNSKPEPIIHRDLKPGNILLDRN+VSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH
Sbjct: 481 LLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 540

Query: 541 YMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPHGLLLAVENSVASASLADILDKSITD 600
           YMDPEYQRTGTLRP SDTYALGVT+LQLLTGRQPHGLLLAVENS+ASASLADILDKSITD
Sbjct: 541 YMDPEYQRTGTLRPNSDTYALGVTILQLLTGRQPHGLLLAVENSIASASLADILDKSITD 600

Query: 601 WPLAEAEELARLALKCMKLRCRDRPDLETEVLPVLKRLVDFADTCQNEEKGSANPPSHYF 660
           WP+AEAEELA LALKC+KLRCRDRPDLETEVLPVLKRLVDFADT Q   KG+ANPPSHYF
Sbjct: 601 WPVAEAEELAGLALKCLKLRCRDRPDLETEVLPVLKRLVDFADTFQ---KGAANPPSHYF 660

Query: 661 CPILQEIMEDPYIAADGFSYEYIAIKAWLEEHDISPVTKLKLLHSMFIPNHTLRSAIQEW 720
           CPILQE+MEDPYIAADGF+YEY AIKAWLE++D+SPVTKLKL HSMFIPN+TLRSAIQEW
Sbjct: 661 CPILQEVMEDPYIAADGFTYEYEAIKAWLEKYDVSPVTKLKLQHSMFIPNYTLRSAIQEW 720

Query: 721 RSRVTFPSS 730
           RSR+TF SS
Sbjct: 721 RSRLTFSSS 726

BLAST of Spg003573 vs. ExPASy TrEMBL
Match: A0A6J1KJP8 (E3 ubiquitin ligase OS=Cucurbita maxima OX=3661 GN=LOC111493807 PE=4 SV=1)

HSP 1 Score: 1298.9 bits (3360), Expect = 0.0e+00
Identity = 662/729 (90.81%), Postives = 695/729 (95.34%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVA+NGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGD VAV
Sbjct: 1   MTSVAVAINGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQ+FVPFKK+CRREKFLLLVETLILEDDNPATAL+RYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQIFVPFKKMCRREKFLLLVETLILEDDNPATALMRYASES 120

Query: 121 GIKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVTRMASTALSTETD 180
           GIK LVLGSCFRTCIAR+LKGDSVPSA++R A    DIYVIYRRRV+TR A+TA S+ETD
Sbjct: 121 GIKHLVLGSCFRTCIARRLKGDSVPSAIMRAASRCFDIYVIYRRRVITRTATTAPSSETD 180

Query: 181 SRQWMLGDTDYYRGSSAVSEKSSGTFSSSSLSIVHRRGDSLEVTSTEQPNSLSTLTEEED 240
           SRQWMLGDTDYYRGSS VSEKS GT SSSS+S VHRRGDSLEV STEQ NSLSTLTEEED
Sbjct: 181 SRQWMLGDTDYYRGSSTVSEKSFGTLSSSSMSNVHRRGDSLEVNSTEQLNSLSTLTEEED 240

Query: 241 MRAEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREETFRKL 300
           M+AEVESLQLELE+TVSLYKQACEELVHAQKKVQSLTSECLEESR+VNAALERE+  RK+
Sbjct: 241 MQAEVESLQLELESTVSLYKQACEELVHAQKKVQSLTSECLEESRKVNAALEREQALRKV 300

Query: 301 AAEEKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRY 360
           AAEEK KHLEAIKELEEAKD LAKEAYERQLAELNALKESLEK+KIVDTLLTNDRRYRRY
Sbjct: 301 AAEEKAKHLEAIKELEEAKDSLAKEAYERQLAELNALKESLEKKKIVDTLLTNDRRYRRY 360

Query: 361 TTAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPVAIKVFQHDIFEKKDEFLKEVEILS 420
           TTAEIE ATNFFDEVNVIGEGGYGKVY CSLDHTPVAIKVF+HDIFE KDEFLKEVEILS
Sbjct: 361 TTAEIEAATNFFDEVNVIGEGGYGKVYVCSLDHTPVAIKVFRHDIFENKDEFLKEVEILS 420

Query: 421 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKTPLPWSIRFRIIFQVASGLA 480
           QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGK PLPWSIR RI+FQVASGLA
Sbjct: 421 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKPPLPWSIRLRIVFQVASGLA 480

Query: 481 FLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 540
           FLHNSKPEPIIHRDLKPGNILLDRN+VSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH
Sbjct: 481 FLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 540

Query: 541 YMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPHGLLLAVENSVASASLADILDKSITD 600
           YMDPEYQRTGTLRPKSDTYALGVT+LQLLTGRQPHGLLLAVENS+AS SLADILDKSITD
Sbjct: 541 YMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAVENSIASGSLADILDKSITD 600

Query: 601 WPLAEAEELARLALKCMKLRCRDRPDLETEVLPVLKRLVDFADTCQNEEKGSANPPSHYF 660
           WPLAEAEELA LALKC+KLRCRDRPDLETEVLPVLKRLVDFAD  +   KG+ANPPSHYF
Sbjct: 601 WPLAEAEELAGLALKCLKLRCRDRPDLETEVLPVLKRLVDFADAFR---KGAANPPSHYF 660

Query: 661 CPILQEIMEDPYIAADGFSYEYIAIKAWLEEHDISPVTKLKLLHSMFIPNHTLRSAIQEW 720
           CPILQE+MEDPYIAADGF+YEY AIKAWLE++D+SPVTKLKL HSMFIPN+TLRSAIQEW
Sbjct: 661 CPILQEVMEDPYIAADGFTYEYEAIKAWLEKYDVSPVTKLKLQHSMFIPNYTLRSAIQEW 720

Query: 721 RSRVTFPSS 730
           RSR+TF SS
Sbjct: 721 RSRLTFSSS 726

BLAST of Spg003573 vs. ExPASy TrEMBL
Match: A0A6J1DK63 (E3 ubiquitin ligase OS=Momordica charantia OX=3673 GN=LOC111021851 PE=4 SV=1)

HSP 1 Score: 1291.6 bits (3341), Expect = 0.0e+00
Identity = 655/729 (89.85%), Postives = 692/729 (94.92%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVAVNG RG  GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV
Sbjct: 1   MTSVAVAVNGGRG--GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120
           SELDADVVALYVHDVKEKYEQVF PFKKLCRREKFLLLVETL+LEDDNPATALLRY SES
Sbjct: 61  SELDADVVALYVHDVKEKYEQVFAPFKKLCRREKFLLLVETLVLEDDNPATALLRYVSES 120

Query: 121 GIKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVTRMASTALSTETD 180
           GIKRLVLGSCFR+CIARKLKG SVPSA+LR AP+S DIYVIY+ RV+TR  +TA STETD
Sbjct: 121 GIKRLVLGSCFRSCIARKLKGPSVPSAILRAAPASFDIYVIYKGRVITRPTATAPSTETD 180

Query: 181 SRQWMLGDTDYYRGSSAVSEKSSGTFSSSSLSIVHRRGDSLEVTSTEQPNSLSTLTEEED 240
           SRQWMLGDTDYYRGS A+SE+SS TFSSSS+S VH+RGDSLE  STEQ NSLSTLTEEED
Sbjct: 181 SRQWMLGDTDYYRGSGAISEQSSATFSSSSVSNVHQRGDSLEGNSTEQLNSLSTLTEEED 240

Query: 241 MRAEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREETFRKL 300
           MRAEVESLQLELETTVSLYK+ACEELVHAQK+VQSLTSECLEESRRV+ ALERE+  R +
Sbjct: 241 MRAEVESLQLELETTVSLYKRACEELVHAQKQVQSLTSECLEESRRVSEALEREQALRTV 300

Query: 301 AAEEKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRY 360
           A+EEK KHLEA+KELEEAKDLLAKEAYERQLA+LNALKES+EKQKIVDTLLTNDRRYRRY
Sbjct: 301 ASEEKAKHLEAMKELEEAKDLLAKEAYERQLADLNALKESVEKQKIVDTLLTNDRRYRRY 360

Query: 361 TTAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPVAIKVFQHDIFEKKDEFLKEVEILS 420
           T AEIE ATNFF++VNVIGEGGYGKVY+CSLDHTPVAIKV QHD+ EKKDEFLKEVEILS
Sbjct: 361 TPAEIEAATNFFEQVNVIGEGGYGKVYRCSLDHTPVAIKVLQHDVLEKKDEFLKEVEILS 420

Query: 421 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKTPLPWSIRFRIIFQVASGLA 480
           QIRHPHVVLLLGACPE+GCLIYEYMENGSLDDHILLRNGKTPLPW +RFRI FQVASGLA
Sbjct: 421 QIRHPHVVLLLGACPEQGCLIYEYMENGSLDDHILLRNGKTPLPWFVRFRIAFQVASGLA 480

Query: 481 FLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 540
           FLHNS PEPIIHRDLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH
Sbjct: 481 FLHNSNPEPIIHRDLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 540

Query: 541 YMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPHGLLLAVENSVASASLADILDKSITD 600
           YMDPEYQRTGTLRPKSDTYALGVT+LQLLTGRQPHGLLLAVE+S+AS+SL DILD+SITD
Sbjct: 541 YMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAVESSIASSSLGDILDRSITD 600

Query: 601 WPLAEAEELARLALKCMKLRCRDRPDLETEVLPVLKRLVDFADTCQNEEKGSANPPSHYF 660
           WPLAEAE LARLALKC+KLRCRDRPDL+TEVLPVLKRLVDFAD CQNEEK  ANPPSHYF
Sbjct: 601 WPLAEAEVLARLALKCLKLRCRDRPDLDTEVLPVLKRLVDFADACQNEEKCPANPPSHYF 660

Query: 661 CPILQEIMEDPYIAADGFSYEYIAIKAWLEEHDISPVTKLKLLHSMFIPNHTLRSAIQEW 720
           CPILQEIM DPYIAADGF+YEY+AIKAWLE+HD+SPVTKLKL HSMFIPNHTLRSAIQEW
Sbjct: 661 CPILQEIMNDPYIAADGFTYEYVAIKAWLEKHDLSPVTKLKLQHSMFIPNHTLRSAIQEW 720

Query: 721 RSRVTFPSS 730
           RSRVTFPSS
Sbjct: 721 RSRVTFPSS 727

BLAST of Spg003573 vs. ExPASy TrEMBL
Match: A0A1S3C9U0 (E3 ubiquitin ligase OS=Cucumis melo OX=3656 GN=LOC103498614 PE=4 SV=1)

HSP 1 Score: 1291.6 bits (3341), Expect = 0.0e+00
Identity = 656/729 (89.99%), Postives = 690/729 (94.65%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRF+LVHVMPKITSIPTPMGD VAV
Sbjct: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFVLVHVMPKITSIPTPMGDLVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120

Query: 121 GIKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVTRMASTALSTETD 180
           GIK LVLGSCFRTCIARKLKGDSVPSA++RTA SS DIYV Y+RRV+TR ASTA STETD
Sbjct: 121 GIKCLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 180

Query: 181 SRQWMLGDTDYYRGSSAVSEKSSGTFSSSSLSIVHRRGDSLEVTSTEQPNSLSTLTEEED 240
           SRQWMLGDTDYY GSSAVS KS GT S SSLSI H+RGDS+ V STEQ NSL TLTEEED
Sbjct: 181 SRQWMLGDTDYYMGSSAVSAKSLGTLSLSSLSISHQRGDSIGVDSTEQLNSLHTLTEEED 240

Query: 241 MRAEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREETFRKL 300
           M+AEVESLQLELETTVSLYKQACEELVH QKKVQSLTSE LEESR+V AALEREE+ RK 
Sbjct: 241 MQAEVESLQLELETTVSLYKQACEELVHTQKKVQSLTSEYLEESRKVTAALEREESLRKA 300

Query: 301 AAEEKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRY 360
           AA+EK KHLEAIKELEEAKDLLAKEAYERQLAELNA+KES+EK+KIVDTLLTNDRRYRRY
Sbjct: 301 AAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELNAIKESVEKEKIVDTLLTNDRRYRRY 360

Query: 361 TTAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPVAIKVFQHDIFEKKDEFLKEVEILS 420
           TTAEIE ATNFF+EVNVIGEGGYGKVYKCSLDHTPVAIKV QHDI+EKKDEFLKEVEILS
Sbjct: 361 TTAEIEAATNFFNEVNVIGEGGYGKVYKCSLDHTPVAIKVIQHDIYEKKDEFLKEVEILS 420

Query: 421 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKTPLPWSIRFRIIFQVASGLA 480
           QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHI LRNGK PLPWS RFRI+FQVASGLA
Sbjct: 421 QIRHPHVVLLLGACPERGCLIYEYMENGSLDDHIFLRNGKAPLPWSTRFRIVFQVASGLA 480

Query: 481 FLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLH 540
           FLHNSKPEPIIHRDLKPGNILLDRN+VSKISDVGMAKIIGDIVPDNVTEYRNT+LAGTLH
Sbjct: 481 FLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTVLAGTLH 540

Query: 541 YMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPHGLLLAVENSVASASLADILDKSITD 600
           YMDPEYQRTGTLRPKSDTYALGVT+LQLLTGRQPHGLLLA+ENS+ASASLADILDKSI+D
Sbjct: 541 YMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSISD 600

Query: 601 WPLAEAEELARLALKCMKLRCRDRPDLETEVLPVLKRLVDFADTCQNEEKGSANPPSHYF 660
           WPLA+AEELARLALKC+KLRCRDRPDLE+EVLPVLKRLVDFADT Q E+KG  NPPSHYF
Sbjct: 601 WPLAKAEELARLALKCLKLRCRDRPDLESEVLPVLKRLVDFADTFQKEDKGFGNPPSHYF 660

Query: 661 CPILQEIMEDPYIAADGFSYEYIAIKAWLEEHDISPVTKLKLLHSMFIPNHTLRSAIQEW 720
           CPILQE+MEDPYIAADGF+YEY+AIKAWLE+HD+SPVTKLKL HS   PNHTLRSAI+EW
Sbjct: 661 CPILQEVMEDPYIAADGFTYEYVAIKAWLEKHDVSPVTKLKLQHSFLXPNHTLRSAIREW 720

Query: 721 RSRVTFPSS 730
           RSR T  SS
Sbjct: 721 RSRATLSSS 729

BLAST of Spg003573 vs. ExPASy TrEMBL
Match: A0A5D3CHP5 (E3 ubiquitin ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001070 PE=4 SV=1)

HSP 1 Score: 1284.6 bits (3323), Expect = 0.0e+00
Identity = 659/747 (88.22%), Postives = 692/747 (92.64%), Query Frame = 0

Query: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 60
           MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGD VAV
Sbjct: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 61  SELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120
           SELDADVVALYVHDVK+KYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120

Query: 121 GIKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVTRMASTALSTETD 180
           GIK LVLGSCFRTCIARKLKGDSVPSA++RTA SS DIYV Y+RRV+TR ASTA STETD
Sbjct: 121 GIKCLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 180

Query: 181 SRQWMLGDTDYYRGSSAVSEKSSGTFSSSSLSIVHRRGDSLEVTSTEQPNSLSTLTEE-- 240
           SRQWMLGDTDYY GSSAVS KS GT S SSLSI H+RGDS+ V STEQ NSL TLTEE  
Sbjct: 181 SRQWMLGDTDYYMGSSAVSAKSLGTLSLSSLSISHQRGDSIGVDSTEQLNSLHTLTEEVF 240

Query: 241 ----------------EDMRAEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSECLE 300
                           EDM+AEVESLQLELETTVSLYKQACEELVH QKKVQSLTSE LE
Sbjct: 241 VFLFYCFIVSSFASISEDMQAEVESLQLELETTVSLYKQACEELVHTQKKVQSLTSEYLE 300

Query: 301 ESRRVNAALEREETFRKLAAEEKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKESLE 360
           ESR+V AALEREE+ RK AA+EK KHLEAIKELEEAKDLLAKEAYERQLAELNA+KES+E
Sbjct: 301 ESRKVTAALEREESLRKAAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELNAIKESVE 360

Query: 361 KQKIVDTLLTNDRRYRRYTTAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPVAIKVFQ 420
           K+KIVDTLLTNDRRYRRYTTAEIE ATNFF+EVNVIGEGGYGKVYKCSLDHTPVAIKV Q
Sbjct: 361 KEKIVDTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKCSLDHTPVAIKVIQ 420

Query: 421 HDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKTP 480
           HDI+EKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHI LRNGK P
Sbjct: 421 HDIYEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHIFLRNGKAP 480

Query: 481 LPWSIRFRIIFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMAKIIGDI 540
           LPWS RFRI+FQVASGLAFLHNSKPEPIIHRDLKPGNILLDRN+VSKISDVGMAKIIGDI
Sbjct: 481 LPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDI 540

Query: 541 VPDNVTEYRNTILAGTLHYMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPHGLLLAVE 600
           VPDNVTEYRNT+LAGTLHYMDPEYQRTGTLRPKSDTYALGVT+LQLLTGRQPHGLLLA+E
Sbjct: 541 VPDNVTEYRNTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIE 600

Query: 601 NSVASASLADILDKSITDWPLAEAEELARLALKCMKLRCRDRPDLETEVLPVLKRLVDFA 660
           NS+ASASLADILDKSI+DWPLA+AEELARLALKC+KLRCRDRPDLE+EVLPVLKRLVDFA
Sbjct: 601 NSIASASLADILDKSISDWPLAKAEELARLALKCLKLRCRDRPDLESEVLPVLKRLVDFA 660

Query: 661 DTCQNEEKGSANPPSHYFCPILQEIMEDPYIAADGFSYEYIAIKAWLEEHDISPVTKLKL 720
           DT Q E+KG  NPPSHYFCPILQE+MEDPYIAADGF+YEY+AIKAWL++HD+SPVTKLKL
Sbjct: 661 DTFQKEDKGFGNPPSHYFCPILQEVMEDPYIAADGFTYEYVAIKAWLDKHDVSPVTKLKL 720

Query: 721 LHSMFIPNHTLRSAIQEWRSRVTFPSS 730
            HS FIPNHTLRSAIQEWRSR T  SS
Sbjct: 721 QHSFFIPNHTLRSAIQEWRSRATSSSS 747

BLAST of Spg003573 vs. TAIR 10
Match: AT5G57035.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 802.4 bits (2071), Expect = 3.1e-232
Identity = 427/764 (55.89%), Postives = 548/764 (71.73%), Query Frame = 0

Query: 2   TSVAVAVNG-VRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDRVAV 61
           +SV+VAV G V    GG  SRRA+RW +EN LP  DR +LVHVMP +T+IP+P G ++ +
Sbjct: 19  SSVSVAVKGSVGDAVGGTASRRALRWTIENFLPKIDRLVLVHVMPTVTTIPSPSGSKIPI 78

Query: 62  SELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 121
            ELD  VV++Y  D+++++EQVFVPFK++C+  K    VETL+LE  +PA ALL+Y S++
Sbjct: 79  EELDESVVSMYKRDLRKEFEQVFVPFKRICKSNK----VETLLLEHHDPAKALLKYMSDT 138

Query: 122 GIKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVTRMASTALSTETD 181
            ++ LV+GSC    + RK KG  +P  VL  AP + +IYV+ + R++T+  +   +  + 
Sbjct: 139 DVECLVIGSCSSNFLTRK-KGQEMPLTVLGEAPETCEIYVVCKDRILTKSTNQFTADSSS 198

Query: 182 SRQWMLGDTDYYR----------GSSAVSEKSSGTF---------------------SSS 241
           S +   G   Y            G SA S  SSG                        SS
Sbjct: 199 SFRIPEGAEAYTESFSRTRSDKTGLSASSITSSGRMRIGRPGSLPHSHPTSRVYSDAQSS 258

Query: 242 SLSIV-----HRRG---DSLEVTSTEQPNSLSTL-TEEEDMRAEVESLQLELETTVSLYK 301
           S  IV     H R     S   TS  Q +    L T +  +RAEVE L+ E++TT+S+YK
Sbjct: 259 STDIVLVDDEHCRSILRHSTVSTSKIQMDPRPHLKTPKSGVRAEVEQLRKEVQTTLSMYK 318

Query: 302 QACEELVHAQKKVQSLTSECLEESRRVNAALEREETFRKLAAEEKEKHLEAIKELEEAKD 361
           QACEELVH Q +VQSL+SEC++E+ RV  ALE+EE  RK AAEEKEKHL+A+KE+EEAK 
Sbjct: 319 QACEELVHKQTQVQSLSSECIKETERVITALEKEEMRRKAAAEEKEKHLKAVKEVEEAKS 378

Query: 362 LLAKEAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRYTTAEIETATNFFDEVNVIGE 421
           +LAKE  ERQLAEL+ALK+S+EKQK+++ L   D RYR+YT  EI  AT+ F    +IGE
Sbjct: 379 MLAKEFCERQLAELDALKQSIEKQKVIEQLFLRDGRYRKYTKEEIAAATDNFSSRKIIGE 438

Query: 422 GGYGKVYKCSLDHTPVAIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCL 481
           GGYGKVYKCSLDHTPVA+KV + D  EKK+EFLKE+ +LSQ+RHPHVVLLLGACPE GCL
Sbjct: 439 GGYGKVYKCSLDHTPVALKVLKPDSVEKKEEFLKEISVLSQLRHPHVVLLLGACPENGCL 498

Query: 482 IYEYMENGSLDDHILLRNGKTPLPWSIRFRIIFQVASGLAFLHNSKPEPIIHRDLKPGNI 541
           +YEYMENGSLD HI  + GK  L W IRFRII++ A GLAFLHNSKPEPI+HRDLKPGNI
Sbjct: 499 VYEYMENGSLDCHISPKKGKPSLSWFIRFRIIYETACGLAFLHNSKPEPIVHRDLKPGNI 558

Query: 542 LLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLHYMDPEYQRTGTLRPKSDTYA 601
           LLDRN+VSKI DVG+AK++ D  PD+VT YRN+I+AGTL+YMDPEYQRTGT+RPKSD YA
Sbjct: 559 LLDRNFVSKIGDVGLAKLMSDEAPDSVTVYRNSIIAGTLYYMDPEYQRTGTIRPKSDLYA 618

Query: 602 LGVTMLQLLTGRQPHGLLLAVENSVASASLADILDKSITDWPLAEAEELARLALKCMKLR 661
            G+ +LQLLT R P+GLL  VE++V      D+LD S+ DWP+AEA+ELAR+A++C +L+
Sbjct: 619 FGIIILQLLTARHPNGLLFCVEDAVKRGCFEDMLDGSVKDWPIAEAKELARIAIRCSQLK 678

Query: 662 CRDRPDLETEVLPVLKRLVDFADTCQNEEKGSANPPSHYFCPILQEIMEDPYIAADGFSY 721
           CRDRPDL T+VLP LKR+++ A++    E+ +A  P+HY+CPIL+EIMEDP IAADGF+Y
Sbjct: 679 CRDRPDLSTQVLPALKRILESANSRLKTEQANARAPTHYYCPILKEIMEDPQIAADGFTY 738

Query: 722 EYIAIKAWLEEH-DISPVTKLKLLHSMFIPNHTLRSAIQEWRSR 724
           E  AIK W+++H D+SPVTK +L HS   PNHTLRSAI+EWRSR
Sbjct: 739 ERKAIKEWIQKHQDVSPVTKHRLKHSDLTPNHTLRSAIREWRSR 777

BLAST of Spg003573 vs. TAIR 10
Match: AT2G19410.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 737.3 bits (1902), Expect = 1.2e-212
Identity = 409/807 (50.68%), Postives = 528/807 (65.43%), Query Frame = 0

Query: 4   VAVAVNGVRGGK-GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTP--------- 63
           VAVAV G+ G K GG GSRRAVRWAV+NLLP AD+F+++HV+P ITSIPTP         
Sbjct: 24  VAVAVKGLIGDKLGGAGSRRAVRWAVDNLLPKADKFVMIHVIPTITSIPTPNILILMFTR 83

Query: 64  -----MGDRVAVSELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDN 123
                 GDR+ V E++  VV +YV DVK++YE VFVPF K+C+                 
Sbjct: 84  MWVVTAGDRLPVEEVEESVVEMYVRDVKKEYETVFVPFLKMCK----------------- 143

Query: 124 PATALLRYASESGIKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVT 183
                    S    KR      FR   +R+ KG  VP  VLR AP + ++Y++ + R+ T
Sbjct: 144 ---------STRSTKRY-----FR---SRRTKGTGVPLTVLRYAPETCEVYIVCKDRITT 203

Query: 184 RMASTALSTETDS------------RQWMLG----------------DTDYYRGSSA--- 243
           +     ++ E  +            R W                   +    R +SA   
Sbjct: 204 KSMDPLINREPCTSPHAAATAHDFLRDWAASFHTLRSPTLPDPRQSTEAGTRRSASAREL 263

Query: 244 ------------VSEKSSGTFSSSSLSIVHRRGDS---------LEVTSTEQPNSLSTLT 303
                        + +SS   S+++  I  RR  S          + T T+  +++  + 
Sbjct: 264 RFEALSLTCNKPKTPQSSKASSATTPEIFRRRRGSDIPQLNYSDFDKTCTKPQSNVENIV 323

Query: 304 EEE-----------------DMRAEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSE 363
            E                  ++  EVE L+ EL++TV  YKQACEEL   Q KV+ L++E
Sbjct: 324 SEHRDSDRSPPETSRKSKKVEIEEEVERLKNELQSTVFKYKQACEELFSTQNKVKMLSTE 383

Query: 364 CLEESRRVNAALEREETFRKLAAEEKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKE 423
            L ES+RVN A+E+EE  R  AA EKE++++A+KE+E AK LLA+E  +RQ+AE+NAL+ 
Sbjct: 384 YLNESKRVNNAVEKEELQRNTAALEKERYMKAVKEVETAKALLAREFCQRQIAEVNALRT 443

Query: 424 SLEKQKIVDTLLTNDRRYRRYTTAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPVAIK 483
            LEK+K++D LL  D RYR+YT  EI TAT  F    VIGEGGYGKVY+CSLD TP A+K
Sbjct: 444 YLEKKKVIDQLLGTDHRYRKYTIEEIVTATEGFSPEKVIGEGGYGKVYQCSLDSTPAAVK 503

Query: 484 VFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNG 543
           V + D  EKK EFLKEVE+LSQ+RHPHVVLLLGACPE GCL+YEY+ENGSL+++I  R  
Sbjct: 504 VVRLDTPEKKQEFLKEVEVLSQLRHPHVVLLLGACPENGCLVYEYLENGSLEEYIFHRKN 563

Query: 544 KTPLPWSIRFRIIFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMAKII 603
           K PLPW IRFR+IF+VA GLAFLH+SKPEPI+HRDLKPGNILL+RNYVSKI+DVG+AK++
Sbjct: 564 KPPLPWFIRFRVIFEVACGLAFLHSSKPEPIVHRDLKPGNILLNRNYVSKIADVGLAKLV 623

Query: 604 GDIVPDNVTEYRNTILAGTLHYMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPHGLLL 663
            D+ PDNVT YRN++LAGTLHY+DPEY RTGT+RPKSD YA G+ +LQLLT R P G++ 
Sbjct: 624 TDVAPDNVTMYRNSVLAGTLHYIDPEYHRTGTIRPKSDLYAFGIIILQLLTARNPSGIVP 683

Query: 664 AVENSVASASLADILDKSITDWPLAEAEELARLALKCMKLRCRDRPDLETEVLPVLKRLV 723
           AVEN+V   +L ++LDKS+TDWPLAE EELAR+ LKC + RCRDRPDL++EV+PVLKRLV
Sbjct: 684 AVENAVKKGTLTEMLDKSVTDWPLAETEELARIGLKCAEFRCRDRPDLKSEVIPVLKRLV 743

Query: 724 DFADTCQNEEKGSANPPSHYFCPILQEIMEDPYIAADGFSYEYIAIKAWLEEHDISPVTK 727
           + A++   +E  +   PSHYFCPIL+EIME+P IAADGF+YE  AI AWLE+H+ISPVT+
Sbjct: 744 ETANSKVKKEGSNLRAPSHYFCPILREIMEEPEIAADGFTYERKAILAWLEKHNISPVTR 796

BLAST of Spg003573 vs. TAIR 10
Match: AT5G61550.2 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 490.0 bits (1260), Expect = 3.4e-138
Identity = 318/838 (37.95%), Postives = 454/838 (54.18%), Query Frame = 0

Query: 3   SVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTA-DRFILVHVMPKITSIPTPMGDRVAVS 62
           SVAVA+NG +       S+  V WA+E  +P     F L++V P ++ IPTPMG  VAVS
Sbjct: 23  SVAVAINGKK------KSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVS 82

Query: 63  ELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASESG 122
           EL  DVV+ Y  ++     ++  P+KK+  R K  + VE L+L+   PA A+    + +G
Sbjct: 83  ELREDVVSAYKQELDWSANEMLRPYKKMFERRK--VQVEVLLLDSLEPAAAIAEEIAGTG 142

Query: 123 IKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVT------------- 182
           + +LV+G   R   +RK+    + S +    P    +YVI + ++ +             
Sbjct: 143 VTKLVIGMSLRGFFSRKI---DMSSLIATAVPRFCTVYVISKGKLASVRPSESDASGSIR 202

Query: 183 --RMASTALST-----------------ETDSRQWMLGDT-DYYRGSSAVSE---KSSGT 242
             R +ST+ ST                 E  SR         +  GS+AV++    SSGT
Sbjct: 203 FERSSSTSGSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSSGT 262

Query: 243 -----FSSSSLSIVH-------RRGDSLEVT---STEQPNSLSTLT---EEEDMRA---- 302
                 +   + IVH        + +S   +    TE  NS+S  +   + ED R     
Sbjct: 263 DQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRREMRSS 322

Query: 303 --------------------------------------------------EVESLQLELE 362
                                                             E+E L+ EL+
Sbjct: 323 SSSNNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRAELK 382

Query: 363 TTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREETFRKLAAEEKEKHLEAIK 422
               +Y  A  E V A KK+  L     EES ++    E+EE  +  A++EK+++ EA+K
Sbjct: 383 HVQEMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEKEEVAKDTASKEKQRYEEAMK 442

Query: 423 ELEEAKDLLAKEAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRYTTAEIETATNFFD 482
           E E+ K+L+ KEA  R+ AE  A +++ EK K+  +L++   +Y+ YT  EI  AT+ F 
Sbjct: 443 EAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQHYTWEEIAAATSDFA 502

Query: 483 EVNVIGEGGYGKVYKCSLDHTPVAIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGA 542
           E   IG G YG VYKC+L HT  A+KV      +   +F +E+EILS+IRHPH+VLLLGA
Sbjct: 503 ENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRHPHLVLLLGA 562

Query: 543 CPERGCLIYEYMENGSLDDHILLRNGKTPLPWSIRFRIIFQVASGLAFLHNSKPEPIIHR 602
           CPERGCL+YEYM+NGSLDD ++L N   P+PW  RFRI  +VAS L FLH SKP PIIHR
Sbjct: 563 CPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASALVFLHKSKPRPIIHR 622

Query: 603 DLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLHYMDPEYQRTGTLR 662
           DLKPGNILLD N+VSK+ DVG++ ++      + T ++ T   GTL Y+DPEYQRTG + 
Sbjct: 623 DLKPGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGTLCYIDPEYQRTGIIS 682

Query: 663 PKSDTYALGVTMLQLLTGRQPHGLLLAVENSVA-SASLADILDKSITDWPLAEAEELARL 722
           PKSD Y+LGV +LQL+T +    +   VE ++   A    ILDK    WP+++  ELA L
Sbjct: 683 PKSDVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKKAGSWPISDTRELAAL 742

Query: 723 ALKCMKLRCRDRPDLETEVLPVLKRLVDFADTCQN-EEKGSANPPSHYFCPILQEIMEDP 728
            L C ++R RDRPDL+ +++P L+RL   AD  QN   +  + PPSH+ CP+L+ +M +P
Sbjct: 743 GLCCTEMRRRDRPDLKDQIIPALERLRKVADKAQNLLSRTPSGPPSHFICPLLKGVMNEP 802

BLAST of Spg003573 vs. TAIR 10
Match: AT5G61550.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 487.3 bits (1253), Expect = 2.2e-137
Identity = 315/831 (37.91%), Postives = 450/831 (54.15%), Query Frame = 0

Query: 3   SVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTA-DRFILVHVMPKITSIPTPMGDRVAVS 62
           SVAVA+NG +       S+  V WA+E  +P     F L++V P ++ IPTPMG  VAVS
Sbjct: 23  SVAVAINGKK------KSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVS 82

Query: 63  ELDADVVALYVHDVKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASESG 122
           EL  DVV+ Y  ++     ++  P+KK+  R K  + VE L+L+   PA A+    + +G
Sbjct: 83  ELREDVVSAYKQELDWSANEMLRPYKKMFERRK--VQVEVLLLDSLEPAAAIAEEIAGTG 142

Query: 123 IKRLVLGSCFRTCIARKLKGDSVPSAVLRTAPSSLDIYVIYRRRVVT------------- 182
           + +LV+G   R   +RK+    + S +    P    +YVI + ++ +             
Sbjct: 143 VTKLVIGMSLRGFFSRKI---DMSSLIATAVPRFCTVYVISKGKLASVRPSESDASGSIR 202

Query: 183 --RMASTALST-----------------ETDSRQWMLGDT-DYYRGSSAVSE---KSSGT 242
             R +ST+ ST                 E  SR         +  GS+AV++    SSGT
Sbjct: 203 FERSSSTSGSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSSGT 262

Query: 243 -----FSSSSLSIVH-------RRGDSLEVT---STEQPNSLSTLT---EEEDMRA---- 302
                 +   + IVH        + +S   +    TE  NS+S  +   + ED R     
Sbjct: 263 DQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRREMRSS 322

Query: 303 --------------------------------------------------EVESLQLELE 362
                                                             E+E L+ EL+
Sbjct: 323 SSSNNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRAELK 382

Query: 363 TTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREETFRKLAAEEKEKHLEAIK 422
               +Y  A  E V A KK+  L     EES ++    E+EE  +  A++EK+++ EA+K
Sbjct: 383 HVQEMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEKEEVAKDTASKEKQRYEEAMK 442

Query: 423 ELEEAKDLLAKEAYERQLAELNALKESLEKQKIVDTLLTNDRRYRRYTTAEIETATNFFD 482
           E E+ K+L+ KEA  R+ AE  A +++ EK K+  +L++   +Y+ YT  EI  AT+ F 
Sbjct: 443 EAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQHYTWEEIAAATSDFA 502

Query: 483 EVNVIGEGGYGKVYKCSLDHTPVAIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGA 542
           E   IG G YG VYKC+L HT  A+KV      +   +F +E+EILS+IRHPH+VLLLGA
Sbjct: 503 ENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRHPHLVLLLGA 562

Query: 543 CPERGCLIYEYMENGSLDDHILLRNGKTPLPWSIRFRIIFQVASGLAFLHNSKPEPIIHR 602
           CPERGCL+YEYM+NGSLDD ++L N   P+PW  RFRI  +VAS L FLH SKP PIIHR
Sbjct: 563 CPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASALVFLHKSKPRPIIHR 622

Query: 603 DLKPGNILLDRNYVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTLHYMDPEYQRTGTLR 662
           DLKPGNILLD N+VSK+ DVG++ ++      + T ++ T   GTL Y+DPEYQRTG + 
Sbjct: 623 DLKPGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGTLCYIDPEYQRTGIIS 682

Query: 663 PKSDTYALGVTMLQLLTGRQPHGLLLAVENSVA-SASLADILDKSITDWPLAEAEELARL 722
           PKSD Y+LGV +LQL+T +    +   VE ++   A    ILDK    WP+++  ELA L
Sbjct: 683 PKSDVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKKAGSWPISDTRELAAL 742

BLAST of Spg003573 vs. TAIR 10
Match: AT4G25160.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 455.3 bits (1170), Expect = 9.3e-128
Identity = 285/809 (35.23%), Postives = 423/809 (52.29%), Query Frame = 0

Query: 16  GGGGSRRAVRWAVENLLPTAD-RFILVHVMPKITSIPTPMGDRVAVSELDADVVALYVHD 75
           G   S+  V WA+E      +  F L+H+ P ITS+PTPMG+ + +SE+  DVV  Y  +
Sbjct: 28  GSSKSKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISEVRDDVVTAYRQE 87

Query: 76  VKEKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASESGIKRLVLGSCFRTC 135
           +  + E++  P+ KL  R K  + VE L++E DN A A+    +   I R+V+G   R+ 
Sbjct: 88  ILWQSEEMLKPYTKLFVRRK--VAVEVLVIESDNVAAAIAEEVTRDSIDRIVIGGSSRSF 147

Query: 136 IARKLKGDSVPSAVL--------------------------------------------- 195
            +RK    SV SA++                                             
Sbjct: 148 FSRKADICSVISALMPNFCTVYVVSKGKLSCVRPSDSDGNATIREDGSERTNSSSGSSGP 207

Query: 196 --------------RTAPSSLDIYVIYRRRVVTRMASTAL-------------------- 255
                         ++ P SL +  +     +   AS  +                    
Sbjct: 208 TSDSSDVMSSAHDSQSRPLSLPVRRMQHFPAIAGQASVPMETSSVGSDETRCMSLDAEEA 267

Query: 256 ---------STETDSRQWMLGDTDYYRGSSAVSEKSSG----------TFSSSSLSIVHR 315
                    ST+T SR W     DY     A+S  SS           ++S   +   H 
Sbjct: 268 RDVSSINRSSTDTTSR-WTPRRRDYEERKEAMSSSSSNREYGNFGTRFSWSGMGVDTTHS 327

Query: 316 RGDSLEVTSTEQPNSLSTLTEEEDMRAEVESLQLELETTVSLYKQACEELVHAQKKVQSL 375
           R        ++  +  S    + ++  EVE L+ EL     +Y  A  E   A +K+  L
Sbjct: 328 RASQQASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYAVAQTETFDASRKLGEL 387

Query: 376 TSECLEESRRVNAALEREETFRKLAAEEKEKHLEAIKELEEAKDLLAKEAYERQLAELNA 435
               LEE+ ++     +E   R+LA +EK+   +A ++ E  ++   +E  +R+ AE  +
Sbjct: 388 NQRRLEEAIKLEELKLKEYEARELAEKEKQNFEKARRDAESMRERAEREIAQRREAERKS 447

Query: 436 LKESLEKQKIVDTLLTNDRRYRRYTTAEIETATNFFDEVNVIGEGGYGKVYKCSLDHTPV 495
            +++ EK+K+  TL +   +Y+ +   EI  AT+ F E   IG G YG VYKC+L HT  
Sbjct: 448 ARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGMGAYGAVYKCNLHHTTA 507

Query: 496 AIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILL 555
            +KV Q    +   +F +E+EILS+IRHPH+VLLLGACPE+G L+YEYMENGSL+D +  
Sbjct: 508 VVKVLQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLFQ 567

Query: 556 RNGKTPLPWSIRFRIIFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNYVSKISDVGMA 615
            N   PLPW  RFRI ++VA+ L FLH SKP+PIIHRDLKP NILLD N+VSK+ DVG++
Sbjct: 568 VNNSPPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGLS 627

Query: 616 KIIG-DIVPDNVTEYRNTILAGTLHYMDPEYQRTGTLRPKSDTYALGVTMLQLLTGRQPH 675
            ++  D +    T Y+ T   GTL Y+DPEYQRTG +  KSD Y+ G+ +LQLLT +   
Sbjct: 628 TMVQVDPLSTKFTIYKQTSPVGTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAKPAI 687

Query: 676 GLLLAVENSV-ASASLADILDKSITDWPLAEAEELARLALKCMKLRCRDRPDLETEVLPV 723
            L   VE+++ ++     ILD+   +WP+ E  ELA LAL C +LR +DRPDL+ ++LP 
Sbjct: 688 ALTHFVESAMDSNDEFLKILDQKAGNWPIEETRELAALALCCTELRGKDRPDLKDQILPA 747

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038891123.10.0e+0092.04U-box domain-containing protein 34-like [Benincasa hispida][more]
KAG6599517.10.0e+0091.08U-box domain-containing protein 34, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023546893.10.0e+0090.81U-box domain-containing protein 34-like [Cucurbita pepo subsp. pepo][more]
XP_022946754.10.0e+0090.67U-box domain-containing protein 34-like [Cucurbita moschata][more]
XP_022999433.10.0e+0090.81U-box domain-containing protein 34-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8S8S71.7e-21150.68U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3... [more]
Q9FKG63.1e-13637.91U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2... [more]
Q9SW111.3e-12635.23U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2... [more]
Q9LU472.8e-12136.38Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q9FKG56.5e-11041.19U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1G4U50.0e+0090.67E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111450728 PE=4 SV=1[more]
A0A6J1KJP80.0e+0090.81E3 ubiquitin ligase OS=Cucurbita maxima OX=3661 GN=LOC111493807 PE=4 SV=1[more]
A0A6J1DK630.0e+0089.85E3 ubiquitin ligase OS=Momordica charantia OX=3673 GN=LOC111021851 PE=4 SV=1[more]
A0A1S3C9U00.0e+0089.99E3 ubiquitin ligase OS=Cucumis melo OX=3656 GN=LOC103498614 PE=4 SV=1[more]
A0A5D3CHP50.0e+0088.22E3 ubiquitin ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G... [more]
Match NameE-valueIdentityDescription
AT5G57035.13.1e-23255.89U-box domain-containing protein kinase family protein [more]
AT2G19410.11.2e-21250.68U-box domain-containing protein kinase family protein [more]
AT5G61550.23.4e-13837.95U-box domain-containing protein kinase family protein [more]
AT5G61550.12.2e-13737.91U-box domain-containing protein kinase family protein [more]
AT4G25160.19.3e-12835.23U-box domain-containing protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 259..279
NoneNo IPR availableCOILSCoilCoilcoord: 298..347
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 351..444
e-value: 1.7E-24
score: 87.7
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 445..654
e-value: 2.2E-47
score: 163.0
NoneNo IPR availablePIRSRPIRSR037993-1PIRSR037993-1coord: 367..575
e-value: 8.6E-6
score: 22.4
NoneNo IPR availablePIRSRPIRSR000604-2PIRSR000604-2coord: 256..635
e-value: 3.3E-22
score: 76.2
NoneNo IPR availablePIRSRPIRSR038172-1PIRSR038172-1coord: 371..568
e-value: 5.7E-14
score: 49.0
NoneNo IPR availablePANTHERPTHR45647:SF65U-BOX DOMAIN-CONTAINING PROTEIN 34coord: 2..214
coord: 225..723
NoneNo IPR availablePANTHERPTHR45647OS02G0152300 PROTEINcoord: 225..723
NoneNo IPR availablePANTHERPTHR45647OS02G0152300 PROTEINcoord: 2..214
NoneNo IPR availableCDDcd01989STK_Ncoord: 3..161
e-value: 7.58885E-39
score: 138.948
NoneNo IPR availableCDDcd16655RING-Ubox_WDSUB1_likecoord: 659..699
e-value: 1.60697E-17
score: 74.5627
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 646..723
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 17..155
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 372..635
e-value: 1.8E-36
score: 137.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 372..635
score: 36.117329
IPR003613U box domainSMARTSM00504Ubox_2coord: 658..720
e-value: 1.1E-24
score: 98.0
IPR003613U box domainPFAMPF04564U-boxcoord: 656..721
e-value: 1.3E-14
score: 54.1
IPR003613U box domainPROSITEPS51698U_BOXcoord: 654..727
score: 30.669807
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 376..628
e-value: 6.0E-42
score: 143.7
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 2..158
e-value: 4.4E-11
score: 45.3
IPR006016UspAPFAMPF00582Uspcoord: 19..154
e-value: 5.1E-7
score: 30.3
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 655..725
e-value: 7.8E-26
score: 91.8
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 490..502
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 378..399
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 355..628

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg003573.1Spg003573.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0004672 protein kinase activity