HG10019298 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10019298
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionbifunctional epoxide hydrolase 2-like
LocationChr04: 19974046 .. 19975923 (-)
RNA-Seq ExpressionHG10019298
SyntenyHG10019298
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACACCATCCACCACACCACCGTTCAAACCAACGGCATCAAACTCCACGTCGCCTCCGCCGGCCCTGTAACCGGCCCACCCGTGCTCCTCCTCCACGGCTTCCCGGAGCTCTGGTACTCCTGGCGCCACCAGATCGTTTTCCTCTCCTCCGTCGGGTACCGAGTCATCGCCCCGGACCTCCGCGGCTACGGTGACTCGGACGCGCCGCCGTCCGCCGAGTCATACACCACCCTGCACATCGTGGGGGATTTGGTGGGGCTGTTGGATGAGCTGGGGATTGAGAAGGTGTTATTGGTGGGCCATGATTGGGGAGCCTTGATTGCTTGGTACTTTTGCTTGTTTAGGCCGGATAAGATCAAGGCGTCGGTGATTTTGAGCGTCCAGTTTTTTCCTAGAAACCCAACAACGCCATTTATAGAAGGCTTCAAAGCTGTTCTTGGAGACCAATTTTATATGGTCAGATTTCAGGTCGTTTTCTAACCATATCCTCCATTTAGTTTTCAACAGTTTTGATCTTAGCTAAAGAGCTGAAAAAAGCTGTTCCTCTATGTTATATGCTATCATAGATTCTTCTAGTTATGTTTGGAAATTAGTGTAGTACAAATTCAAACAGTATTGATGCGACGGAAAATATTAAAAAATAAAAATAAATTTAAAGTTAAATCAGGTCTGTTTGATTCGCTTACTTCTTAAGTTGTGTAAATGTTAGATATCATATAATTTATCTTCTTCCGTTAGCTTAAAATTTTAGGTCAATCGACGATTTAAGATGATACTTTTAGTGGTTCAGGGAGGTTCGGTGTTCAGGTCCCTACAATATTATTTTCACATTTATCGAGCAACTCCTTCTTTCTCTCTATCTTTATATTTCATCTTTCTCAAGTAAATTCATCTTCTTCTCTTTATCTACTTTTCATAACTATTGAAAAACTCTAGTTTACAACTCTACAACTCAAATACAAATTTACTAACTCCAACAATTTAATCTTAAAGAAATTTTTTAATACTACAGAGTATGATACTCTTTTTTTTCACACTATAAATTTGCTTTCATAAATATTTGGAGATCTTACAACAAGTGAAAGTAATAAGGAAAATTATTCATACTACCGTAATATCCGTAATATAGAAGTTTTTTGTATAGCTTCTTATAATGAACTAATTATTGAGTTCATGTATGTTTGGTGTTAAATAGGAACCAGGAAAGGCAGAGGAAGAGTTTGCGTCGGTGGATATAAGAGAGCTTTTCAAGAACGTGCTGTCAAATCGAGACCCACGTGCTCCATATTTGCCTGGTGAGGTCAAATTTGAAGGGGTCCCACCGCCGCCGCTAGCACCGTGGCTCACTCCCCAAGATATCGACTTTTATGCCGACAAGTTCTCCCACACTGGCTTCACCGGCGGCCTCAACTATTACCGAGCTTTCGACCGGTAATAACAATTATCTACATCATTTTCCTAACATTTATGTTCTCCTAATACTTAACCATTTAGAGAAACAAAATATAAATTTCTTTTAATTTTAAATTATAGAATTCCATTTGAAACCAACTCGGAAAGATTGTAACAGTAACATCTTTAATGATTGAAAGGGAAGTGAATGAATGTTTGAGTTTGAAATGCAGGACTTGGGAGCTGACAGCGCCATGGACAGGGGCACAAATAAAAGTACCAGTAAAGTTCATAGTGGGAGATTTAGATCTGACTTACCATTTTCCAGGAGCTCAAAAGTACATCAATGGCCATGCCTTCAAAAAGGATGTGCCTGGTTTGGAAGAAGTGATTGTTATGAAAGATACTTCTCATTTCATCAACCAAGAAAGGCCTCATGAAATCAACTGCTACGTTCATGATTTCTTCAACAAATTCTGTTGA

mRNA sequence

ATGGACACCATCCACCACACCACCGTTCAAACCAACGGCATCAAACTCCACGTCGCCTCCGCCGGCCCTGTAACCGGCCCACCCGTGCTCCTCCTCCACGGCTTCCCGGAGCTCTGGTACTCCTGGCGCCACCAGATCGTTTTCCTCTCCTCCGTCGGGTACCGAGTCATCGCCCCGGACCTCCGCGGCTACGGTGACTCGGACGCGCCGCCGTCCGCCGAGTCATACACCACCCTGCACATCGTGGGGGATTTGGTGGGGCTGTTGGATGAGCTGGGGATTGAGAAGGTGTTATTGGTGGGCCATGATTGGGGAGCCTTGATTGCTTGGTACTTTTGCTTGTTTAGGCCGGATAAGATCAAGGCGTCGGTGATTTTGAGCGTCCAGTTTTTTCCTAGAAACCCAACAACGCCATTTATAGAAGGCTTCAAAGCTGTTCTTGGAGACCAATTTTATATGGAACCAGGAAAGGCAGAGGAAGAGTTTGCGTCGGTGGATATAAGAGAGCTTTTCAAGAACGTGCTGTCAAATCGAGACCCACGTGCTCCATATTTGCCTGGTGAGGTCAAATTTGAAGGGGTCCCACCGCCGCCGCTAGCACCGTGGCTCACTCCCCAAGATATCGACTTTTATGCCGACAAGTTCTCCCACACTGGCTTCACCGGCGGCCTCAACTATTACCGAGCTTTCGACCGGACTTGGGAGCTGACAGCGCCATGGACAGGGGCACAAATAAAAGTACCAGTAAAGTTCATAGTGGGAGATTTAGATCTGACTTACCATTTTCCAGGAGCTCAAAAGTACATCAATGGCCATGCCTTCAAAAAGGATGTGCCTGGTTTGGAAGAAGTGATTGTTATGAAAGATACTTCTCATTTCATCAACCAAGAAAGGCCTCATGAAATCAACTGCTACGTTCATGATTTCTTCAACAAATTCTGTTGA

Coding sequence (CDS)

ATGGACACCATCCACCACACCACCGTTCAAACCAACGGCATCAAACTCCACGTCGCCTCCGCCGGCCCTGTAACCGGCCCACCCGTGCTCCTCCTCCACGGCTTCCCGGAGCTCTGGTACTCCTGGCGCCACCAGATCGTTTTCCTCTCCTCCGTCGGGTACCGAGTCATCGCCCCGGACCTCCGCGGCTACGGTGACTCGGACGCGCCGCCGTCCGCCGAGTCATACACCACCCTGCACATCGTGGGGGATTTGGTGGGGCTGTTGGATGAGCTGGGGATTGAGAAGGTGTTATTGGTGGGCCATGATTGGGGAGCCTTGATTGCTTGGTACTTTTGCTTGTTTAGGCCGGATAAGATCAAGGCGTCGGTGATTTTGAGCGTCCAGTTTTTTCCTAGAAACCCAACAACGCCATTTATAGAAGGCTTCAAAGCTGTTCTTGGAGACCAATTTTATATGGAACCAGGAAAGGCAGAGGAAGAGTTTGCGTCGGTGGATATAAGAGAGCTTTTCAAGAACGTGCTGTCAAATCGAGACCCACGTGCTCCATATTTGCCTGGTGAGGTCAAATTTGAAGGGGTCCCACCGCCGCCGCTAGCACCGTGGCTCACTCCCCAAGATATCGACTTTTATGCCGACAAGTTCTCCCACACTGGCTTCACCGGCGGCCTCAACTATTACCGAGCTTTCGACCGGACTTGGGAGCTGACAGCGCCATGGACAGGGGCACAAATAAAAGTACCAGTAAAGTTCATAGTGGGAGATTTAGATCTGACTTACCATTTTCCAGGAGCTCAAAAGTACATCAATGGCCATGCCTTCAAAAAGGATGTGCCTGGTTTGGAAGAAGTGATTGTTATGAAAGATACTTCTCATTTCATCAACCAAGAAAGGCCTCATGAAATCAACTGCTACGTTCATGATTTCTTCAACAAATTCTGTTGA

Protein sequence

MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPDLRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKIKASVILSVQFFPRNPTTPFIEGFKAVLGDQFYMEPGKAEEEFASVDIRELFKNVLSNRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFINQERPHEINCYVHDFFNKFC
Homology
BLAST of HG10019298 vs. NCBI nr
Match: XP_038905173.1 (epoxide hydrolase A-like [Benincasa hispida])

HSP 1 Score: 630.6 bits (1625), Expect = 7.4e-177
Identity = 293/318 (92.14%), Postives = 303/318 (95.28%), Query Frame = 0

Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           MDTIHHTTVQTNGIKLHVA+AGPVTGPPVLLLHGFPELWYSWRHQIVFLSS GYRVIAPD
Sbjct: 1   MDTIHHTTVQTNGIKLHVAAAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSAGYRVIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           L GYGDSDAP S++SYT LHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI
Sbjct: 61  LHGYGDSDAPSSSDSYTALHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYM----EPGKAEEEFASVDIRELFKNVLS 180
           KASVILSVQFFPRNP TPFIEGFKAVLGDQFYM    EPGKAEEEFA+VDIRE FKN LS
Sbjct: 121 KASVILSVQFFPRNPKTPFIEGFKAVLGDQFYMVRFQEPGKAEEEFATVDIREFFKNTLS 180

Query: 181 NRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRTWEL 240
           NRDPRAPYLPGEVKFEGVPPPPLAPWLTP+DIDFYA KFSHTGFTG LNYYRAFDRTWEL
Sbjct: 181 NRDPRAPYLPGEVKFEGVPPPPLAPWLTPEDIDFYAQKFSHTGFTGSLNYYRAFDRTWEL 240

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFINQ 300
           TAPWTGAQI+VPVKFIVGDLDLTYHFPGAQKYI+G AFKKDVPGL+EVIVMKD SHFINQ
Sbjct: 241 TAPWTGAQIEVPVKFIVGDLDLTYHFPGAQKYIHGDAFKKDVPGLDEVIVMKDASHFINQ 300

Query: 301 ERPHEINCYVHDFFNKFC 315
           ERPHEINC++HDFFNKFC
Sbjct: 301 ERPHEINCHIHDFFNKFC 318

BLAST of HG10019298 vs. NCBI nr
Match: XP_004152243.1 (uncharacterized protein LOC101204575 [Cucumis sativus] >KGN52824.1 hypothetical protein Csa_014427 [Cucumis sativus])

HSP 1 Score: 604.4 bits (1557), Expect = 5.7e-169
Identity = 278/318 (87.42%), Postives = 298/318 (93.71%), Query Frame = 0

Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+TI+H TVQTNGI LHVA+AGPVTGPPVLLLHGFPELWYSWRHQI+FLSSVGYRVIAPD
Sbjct: 1   METINHITVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSVGYRVIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPS+++YT LHIVGD+VGLL+ELGI+KVLLVGHDWGALIAWYFCLFRPD+I
Sbjct: 61  LRGYGDSDAPPSSDTYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYM----EPGKAEEEFASVDIRELFKNVLS 180
           KASVILSVQFFPRNP   F+EGFKAVLGDQFYM    EPGKAE+EFASVDIRE FKNV+S
Sbjct: 121 KASVILSVQFFPRNPKVSFVEGFKAVLGDQFYMVRFQEPGKAEKEFASVDIREFFKNVMS 180

Query: 181 NRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRTWEL 240
           NRDP APYLPGE KFEGVPPP LAPWLTPQDID+YA KFSH+GFTGGLNYYRAFDRTWEL
Sbjct: 181 NRDPSAPYLPGEEKFEGVPPPSLAPWLTPQDIDYYAQKFSHSGFTGGLNYYRAFDRTWEL 240

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFINQ 300
           TAPWT A+IKVPVKFIVGDLDLTYHFPG Q YING AF+KDVPGLEEVIVMKDTSHFINQ
Sbjct: 241 TAPWTAAEIKVPVKFIVGDLDLTYHFPGGQDYINGDAFRKDVPGLEEVIVMKDTSHFINQ 300

Query: 301 ERPHEINCYVHDFFNKFC 315
           ERP EINC++HDFFNKFC
Sbjct: 301 ERPDEINCHIHDFFNKFC 318

BLAST of HG10019298 vs. NCBI nr
Match: KAA0044388.1 (bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa] >TYK29516.1 bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 582.8 bits (1501), Expect = 1.8e-162
Identity = 271/318 (85.22%), Postives = 290/318 (91.19%), Query Frame = 0

Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+TI HT VQTNG+ LHVA+AGPVTGPPVLLLHGFPELWYSWR QI+FLSS GYRVIAPD
Sbjct: 33  METIQHTNVQTNGVNLHVATAGPVTGPPVLLLHGFPELWYSWRSQILFLSSAGYRVIAPD 92

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPS++SYT LHIVGD+VGLLDE GI+KVLLVGHDWGALIAWY CLFRPD+I
Sbjct: 93  LRGYGDSDAPPSSDSYTALHIVGDVVGLLDEFGIDKVLLVGHDWGALIAWYCCLFRPDRI 152

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYM----EPGKAEEEFASVDIRELFKNVLS 180
           KASVILSVQFFPRNP   FIEGFKAVLGDQFYM    EPGKAE+EFASVDIRE FKNV+S
Sbjct: 153 KASVILSVQFFPRNPKMSFIEGFKAVLGDQFYMVRFQEPGKAEKEFASVDIREFFKNVMS 212

Query: 181 NRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRTWEL 240
           NRDP APYLPGEVKFEGVPPP LAPWLTP+DID+YA KFSH+GFTGGLNYYRAFDRTWEL
Sbjct: 213 NRDPSAPYLPGEVKFEGVPPPSLAPWLTPKDIDYYAQKFSHSGFTGGLNYYRAFDRTWEL 272

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFINQ 300
           TAPWT A+IKVPVKFIVGDLDLTYHFPGAQ YING AF+KDVPGLEEVIV+ D SHFINQ
Sbjct: 273 TAPWTKAEIKVPVKFIVGDLDLTYHFPGAQDYINGDAFEKDVPGLEEVIVIGDASHFINQ 332

Query: 301 ERPHEINCYVHDFFNKFC 315
           ERP EIN ++HDFF  FC
Sbjct: 333 ERPVEINSHIHDFFRNFC 350

BLAST of HG10019298 vs. NCBI nr
Match: XP_008454324.2 (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo])

HSP 1 Score: 582.8 bits (1501), Expect = 1.8e-162
Identity = 271/318 (85.22%), Postives = 290/318 (91.19%), Query Frame = 0

Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+TI HT VQTNG+ LHVA+AGPVTGPPVLLLHGFPELWYSWR QI+FLSS GYRVIAPD
Sbjct: 1   METIQHTNVQTNGVNLHVATAGPVTGPPVLLLHGFPELWYSWRSQILFLSSAGYRVIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPS++SYT LHIVGD+VGLLDE GI+KVLLVGHDWGALIAWY CLFRPD+I
Sbjct: 61  LRGYGDSDAPPSSDSYTALHIVGDVVGLLDEFGIDKVLLVGHDWGALIAWYCCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYM----EPGKAEEEFASVDIRELFKNVLS 180
           KASVILSVQFFPRNP   FIEGFKAVLGDQFYM    EPGKAE+EFASVDIRE FKNV+S
Sbjct: 121 KASVILSVQFFPRNPKMSFIEGFKAVLGDQFYMVRFQEPGKAEKEFASVDIREFFKNVMS 180

Query: 181 NRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRTWEL 240
           NRDP APYLPGEVKFEGVPPP LAPWLTP+DID+YA KFSH+GFTGGLNYYRAFDRTWEL
Sbjct: 181 NRDPSAPYLPGEVKFEGVPPPSLAPWLTPKDIDYYAQKFSHSGFTGGLNYYRAFDRTWEL 240

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFINQ 300
           TAPWT A+IKVPVKFIVGDLDLTYHFPGAQ YING AF+KDVPGLEEVIV+ D SHFINQ
Sbjct: 241 TAPWTKAEIKVPVKFIVGDLDLTYHFPGAQDYINGDAFEKDVPGLEEVIVIGDASHFINQ 300

Query: 301 ERPHEINCYVHDFFNKFC 315
           ERP EIN ++HDFF  FC
Sbjct: 301 ERPVEINSHIHDFFRNFC 318

BLAST of HG10019298 vs. NCBI nr
Match: XP_022998019.1 (uncharacterized protein LOC111492796 [Cucurbita maxima])

HSP 1 Score: 569.3 bits (1466), Expect = 2.0e-158
Identity = 258/318 (81.13%), Postives = 290/318 (91.19%), Query Frame = 0

Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+ IHHTTV+TNGI LH+A+ G  TGPPVLLLHGFPE WYSWRHQ+++LSS+GYR IAPD
Sbjct: 1   MEAIHHTTVKTNGINLHIAAVG--TGPPVLLLHGFPEFWYSWRHQLIYLSSIGYRAIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPSA++YTTLHIVGDLVGLLDELGIEKVLLVG+DWGA+IAWY CLFRPD+I
Sbjct: 61  LRGYGDSDAPPSADAYTTLHIVGDLVGLLDELGIEKVLLVGNDWGAIIAWYLCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYM----EPGKAEEEFASVDIRELFKNVLS 180
           KA+VILSVQFFPRNPTTPF+E FK+VLGDQFYM    +PG+AEEEF +VDI++ F  V+S
Sbjct: 121 KAAVILSVQFFPRNPTTPFVEAFKSVLGDQFYMVRFQQPGQAEEEFGTVDIKKFFNEVMS 180

Query: 181 NRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRTWEL 240
           NRDPRAPYLPGEVKFEG+PPPP A WLTP+DID YAD FS TGFTGGLNYYRAFDRTWEL
Sbjct: 181 NRDPRAPYLPGEVKFEGLPPPPPATWLTPEDIDVYADMFSRTGFTGGLNYYRAFDRTWEL 240

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFINQ 300
           TA WT A+IKVPVKFIVGDLDLTYHFPGAQ+YI+G  FK+DVPGLEEV+VMKDTSHFINQ
Sbjct: 241 TAAWTRAEIKVPVKFIVGDLDLTYHFPGAQEYIHGDGFKRDVPGLEEVVVMKDTSHFINQ 300

Query: 301 ERPHEINCYVHDFFNKFC 315
           ERP+E N ++HDFFNKFC
Sbjct: 301 ERPNETNAHIHDFFNKFC 316

BLAST of HG10019298 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.4e-56
Identity = 120/314 (38.22%), Postives = 164/314 (52.23%), Query Frame = 0

Query: 9   VQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPDLRGYGDSD 68
           V TNG++L V  AG    P V+L HGFPEL YSWRHQI  L+  GY V+APD RGYG S 
Sbjct: 9   VDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSS 68

Query: 69  APPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKIKASVILSV 128
            P + E+Y    +  DLVGLLD++G E+ + VGHDWGA++ W   L   D++ A   LSV
Sbjct: 69  RPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALSV 128

Query: 129 QFFPRNPTTPFIEGFKAVLGDQF-----YMEPGKAEEEFASVDIRELFKNVLSNRDP--- 188
              PR    P  + F++  G+ F     + EPG A+ E      R + + +   R P   
Sbjct: 129 PALPRAQVPP-TQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLRPPGDQ 188

Query: 189 ---RAPYLPGEVKFEGVPPPP--LAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRTWE 248
                   PG   F    P P  L  W++ +++D Y  +F+ TGFTGGLN+YR FDR WE
Sbjct: 189 SAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNWYRNFDRNWE 248

Query: 249 LTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFIN 308
            TA   G  I VP  FI G  D    F    +        + + G    +++    H++ 
Sbjct: 249 TTADLAGKTISVPSLFIAGTADPVLTFTRTDR------AAEVISGPYREVLIDGAGHWLQ 308

Query: 309 QERPHEINCYVHDF 310
           QERP E+   + +F
Sbjct: 309 QERPGEVTAALLEF 315

BLAST of HG10019298 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.6e-49
Identity = 109/301 (36.21%), Postives = 161/301 (53.49%), Query Frame = 0

Query: 13  GIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPDLRGYGDSDAPPS 72
           G++LH    G  +GP V L HGFPE W+SWR+QI  L+  G+RV+A D++GYG+S APP 
Sbjct: 247 GVRLHFVEMG--SGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPE 306

Query: 73  AESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKIKASVILSVQFFP 132
            E Y+   +  D+V  L++LG+ + + +GHDWG ++ W   LF P++++A   L+  F P
Sbjct: 307 IEEYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMP 366

Query: 133 RNPTTPFIEGFKA--VLGDQFY-MEPGKAEEEFASVDIRELFKNVLSNRDP------RAP 192
            NP    +E  KA  V   Q Y  EPG AE E    ++   FKN     D       R  
Sbjct: 367 SNPNVSPMEIIKANPVFDYQLYFQEPGVAEAELEQ-NLDRTFKNFFRAHDETFLTTNRVR 426

Query: 193 YLPGEVKFEGVP-PPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRTWELTAPWTG 252
            L G   F G P  P L+  +T +DI FY  +F  +GF G LN+YR  +R W+     +G
Sbjct: 427 ELGG--LFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCKGSG 486

Query: 253 AQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFINQERPHEI 304
            +I +P   +  + DL  H P   K++        +P L+   + KD  H+   ++P E+
Sbjct: 487 RKILIPALMVTAENDLVLH-PKMSKHMENW-----IPHLKRGHI-KDCGHWTQIDKPAEL 535

BLAST of HG10019298 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 191.0 bits (484), Expect = 2.0e-47
Identity = 100/298 (33.56%), Postives = 155/298 (52.01%), Query Frame = 0

Query: 14  IKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPDLRGYGDSDAPPSA 73
           ++LH    G  +GP V L HGFPE WYSWR+QI  L+  GYRV+A D++GYG+S APP  
Sbjct: 248 VRLHFVELG--SGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEI 307

Query: 74  ESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKIKASVILSVQFFPR 133
           E Y    +  ++V  LD+LG+ + + +GHDWG ++ WY  LF P++++A   L+  F P 
Sbjct: 308 EEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPA 367

Query: 134 NPTTPFIEGFKA--VLGDQFY-MEPGKAEEEFASVDIRELFKNVLSNRDPRAPYL----- 193
           NP    +E  KA  V   Q Y  EPG AE E    ++   FK++    D     +     
Sbjct: 368 NPNMSPLESIKANPVFDYQLYFQEPGVAEAELEQ-NLSRTFKSLFRASDESVLSMHKVCE 427

Query: 194 PGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRTWELTAPWTGAQI 253
            G +       P L+  +T ++I FY  +F  +GF G LN+YR  +R W+      G +I
Sbjct: 428 AGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKI 487

Query: 254 KVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFINQERPHEIN 304
            +P   +  + D     P   +++        +P L+   + +D  H+   ++P E+N
Sbjct: 488 LIPALMVTAEKDFVL-VPQMSQHMEDW-----IPHLKRGHI-EDCGHWTQMDKPTEVN 535

BLAST of HG10019298 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 179.1 bits (453), Expect = 7.8e-44
Identity = 102/312 (32.69%), Postives = 160/312 (51.28%), Query Frame = 0

Query: 2   DTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPDL 61
           D  H       GI+LH    G  +GP + L HGFPE W+SWR+QI  L+  G+RV+A D+
Sbjct: 234 DVSHGYVTVKPGIRLHFVEMG--SGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDM 293

Query: 62  RGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKIK 121
           +GYGDS +PP  E Y    +  ++V  LD+LGI + + +GHDW  ++ W   LF P++++
Sbjct: 294 KGYGDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVR 353

Query: 122 ASVILSVQFFPRNPTTPFIEGFKA--VLGDQFY-MEPGKAEEEFASVDIRELFKNVLSNR 181
           A   L+  F P +P    ++  ++  V   Q Y  EPG AE E    ++   FK+     
Sbjct: 354 AVASLNTPFMPPDPDVSPMKVIRSIPVFNYQLYFQEPGVAEAELEK-NMSRTFKSFFRAS 413

Query: 182 DP-------RAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFD 241
           D        +A  + G +       P L+   T ++I+FY  +F  TGF G LN+YR  +
Sbjct: 414 DETGFIAVHKATEI-GGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTE 473

Query: 242 RTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTS 301
           R W+ +    G +I VP   +  + D+    P   K +     +K +P L+   + +D  
Sbjct: 474 RNWKWSCKGLGRKILVPALMVTAEKDIVLR-PEMSKNM-----EKWIPFLKRGHI-EDCG 533

Query: 302 HFINQERPHEIN 304
           H+   E+P E+N
Sbjct: 534 HWTQIEKPTEVN 534

BLAST of HG10019298 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 8.6e-43
Identity = 97/311 (31.19%), Postives = 153/311 (49.20%), Query Frame = 0

Query: 2   DTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPDL 61
           D  H       GI+LH    G  +GP + L HGFPE W+SWR+QI  L+  G+RV+A D+
Sbjct: 234 DVSHGYVTVKPGIRLHFVEMG--SGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDM 293

Query: 62  RGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKIK 121
           +GYGDS +PP  E Y    +  ++V  L++LGI + + +GHDW  ++ W   LF P++++
Sbjct: 294 KGYGDSSSPPEIEEYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVR 353

Query: 122 ASVILSVQFFPRNPTTPFIEGFKA--VLGDQFY-MEPGKAEEEFASVDIRELFKNVLSNR 181
           A   L+    P NP    +E  ++  V   Q Y  EPG AE E    ++   FK+     
Sbjct: 354 AVASLNTPLMPPNPEVSPMEVIRSIPVFNYQLYFQEPGVAEAELEK-NMSRTFKSFFRTS 413

Query: 182 DPRAPYLPGEVK-----FEGVP-PPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDR 241
           D        +         G P  P ++   T ++I++Y  +F  +GF G LN+YR  +R
Sbjct: 414 DDMGLLTVNKATEMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTER 473

Query: 242 TWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSH 301
            W+ +    G +I VP   +  + D+      ++   N   F K          ++D  H
Sbjct: 474 NWKWSCKALGRKILVPALMVTAEKDIVLRPEMSKNMENWIPFLKRGH-------IEDCGH 533

Query: 302 FINQERPHEIN 304
           +   E+P E+N
Sbjct: 534 WTQIEKPAEVN 534

BLAST of HG10019298 vs. ExPASy TrEMBL
Match: A0A0A0KV84 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001910 PE=4 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 2.8e-169
Identity = 278/318 (87.42%), Postives = 298/318 (93.71%), Query Frame = 0

Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+TI+H TVQTNGI LHVA+AGPVTGPPVLLLHGFPELWYSWRHQI+FLSSVGYRVIAPD
Sbjct: 1   METINHITVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSVGYRVIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPS+++YT LHIVGD+VGLL+ELGI+KVLLVGHDWGALIAWYFCLFRPD+I
Sbjct: 61  LRGYGDSDAPPSSDTYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYM----EPGKAEEEFASVDIRELFKNVLS 180
           KASVILSVQFFPRNP   F+EGFKAVLGDQFYM    EPGKAE+EFASVDIRE FKNV+S
Sbjct: 121 KASVILSVQFFPRNPKVSFVEGFKAVLGDQFYMVRFQEPGKAEKEFASVDIREFFKNVMS 180

Query: 181 NRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRTWEL 240
           NRDP APYLPGE KFEGVPPP LAPWLTPQDID+YA KFSH+GFTGGLNYYRAFDRTWEL
Sbjct: 181 NRDPSAPYLPGEEKFEGVPPPSLAPWLTPQDIDYYAQKFSHSGFTGGLNYYRAFDRTWEL 240

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFINQ 300
           TAPWT A+IKVPVKFIVGDLDLTYHFPG Q YING AF+KDVPGLEEVIVMKDTSHFINQ
Sbjct: 241 TAPWTAAEIKVPVKFIVGDLDLTYHFPGGQDYINGDAFRKDVPGLEEVIVMKDTSHFINQ 300

Query: 301 ERPHEINCYVHDFFNKFC 315
           ERP EINC++HDFFNKFC
Sbjct: 301 ERPDEINCHIHDFFNKFC 318

BLAST of HG10019298 vs. ExPASy TrEMBL
Match: A0A5A7TNE2 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001190 PE=4 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 8.6e-163
Identity = 271/318 (85.22%), Postives = 290/318 (91.19%), Query Frame = 0

Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+TI HT VQTNG+ LHVA+AGPVTGPPVLLLHGFPELWYSWR QI+FLSS GYRVIAPD
Sbjct: 33  METIQHTNVQTNGVNLHVATAGPVTGPPVLLLHGFPELWYSWRSQILFLSSAGYRVIAPD 92

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPS++SYT LHIVGD+VGLLDE GI+KVLLVGHDWGALIAWY CLFRPD+I
Sbjct: 93  LRGYGDSDAPPSSDSYTALHIVGDVVGLLDEFGIDKVLLVGHDWGALIAWYCCLFRPDRI 152

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYM----EPGKAEEEFASVDIRELFKNVLS 180
           KASVILSVQFFPRNP   FIEGFKAVLGDQFYM    EPGKAE+EFASVDIRE FKNV+S
Sbjct: 153 KASVILSVQFFPRNPKMSFIEGFKAVLGDQFYMVRFQEPGKAEKEFASVDIREFFKNVMS 212

Query: 181 NRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRTWEL 240
           NRDP APYLPGEVKFEGVPPP LAPWLTP+DID+YA KFSH+GFTGGLNYYRAFDRTWEL
Sbjct: 213 NRDPSAPYLPGEVKFEGVPPPSLAPWLTPKDIDYYAQKFSHSGFTGGLNYYRAFDRTWEL 272

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFINQ 300
           TAPWT A+IKVPVKFIVGDLDLTYHFPGAQ YING AF+KDVPGLEEVIV+ D SHFINQ
Sbjct: 273 TAPWTKAEIKVPVKFIVGDLDLTYHFPGAQDYINGDAFEKDVPGLEEVIVIGDASHFINQ 332

Query: 301 ERPHEINCYVHDFFNKFC 315
           ERP EIN ++HDFF  FC
Sbjct: 333 ERPVEINSHIHDFFRNFC 350

BLAST of HG10019298 vs. ExPASy TrEMBL
Match: A0A1S3BXU7 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494759 PE=4 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 8.6e-163
Identity = 271/318 (85.22%), Postives = 290/318 (91.19%), Query Frame = 0

Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+TI HT VQTNG+ LHVA+AGPVTGPPVLLLHGFPELWYSWR QI+FLSS GYRVIAPD
Sbjct: 1   METIQHTNVQTNGVNLHVATAGPVTGPPVLLLHGFPELWYSWRSQILFLSSAGYRVIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPS++SYT LHIVGD+VGLLDE GI+KVLLVGHDWGALIAWY CLFRPD+I
Sbjct: 61  LRGYGDSDAPPSSDSYTALHIVGDVVGLLDEFGIDKVLLVGHDWGALIAWYCCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYM----EPGKAEEEFASVDIRELFKNVLS 180
           KASVILSVQFFPRNP   FIEGFKAVLGDQFYM    EPGKAE+EFASVDIRE FKNV+S
Sbjct: 121 KASVILSVQFFPRNPKMSFIEGFKAVLGDQFYMVRFQEPGKAEKEFASVDIREFFKNVMS 180

Query: 181 NRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRTWEL 240
           NRDP APYLPGEVKFEGVPPP LAPWLTP+DID+YA KFSH+GFTGGLNYYRAFDRTWEL
Sbjct: 181 NRDPSAPYLPGEVKFEGVPPPSLAPWLTPKDIDYYAQKFSHSGFTGGLNYYRAFDRTWEL 240

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFINQ 300
           TAPWT A+IKVPVKFIVGDLDLTYHFPGAQ YING AF+KDVPGLEEVIV+ D SHFINQ
Sbjct: 241 TAPWTKAEIKVPVKFIVGDLDLTYHFPGAQDYINGDAFEKDVPGLEEVIVIGDASHFINQ 300

Query: 301 ERPHEINCYVHDFFNKFC 315
           ERP EIN ++HDFF  FC
Sbjct: 301 ERPVEINSHIHDFFRNFC 318

BLAST of HG10019298 vs. ExPASy TrEMBL
Match: A0A6J1KD67 (uncharacterized protein LOC111492796 OS=Cucurbita maxima OX=3661 GN=LOC111492796 PE=4 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 9.9e-159
Identity = 258/318 (81.13%), Postives = 290/318 (91.19%), Query Frame = 0

Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+ IHHTTV+TNGI LH+A+ G  TGPPVLLLHGFPE WYSWRHQ+++LSS+GYR IAPD
Sbjct: 1   MEAIHHTTVKTNGINLHIAAVG--TGPPVLLLHGFPEFWYSWRHQLIYLSSIGYRAIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPSA++YTTLHIVGDLVGLLDELGIEKVLLVG+DWGA+IAWY CLFRPD+I
Sbjct: 61  LRGYGDSDAPPSADAYTTLHIVGDLVGLLDELGIEKVLLVGNDWGAIIAWYLCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYM----EPGKAEEEFASVDIRELFKNVLS 180
           KA+VILSVQFFPRNPTTPF+E FK+VLGDQFYM    +PG+AEEEF +VDI++ F  V+S
Sbjct: 121 KAAVILSVQFFPRNPTTPFVEAFKSVLGDQFYMVRFQQPGQAEEEFGTVDIKKFFNEVMS 180

Query: 181 NRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRTWEL 240
           NRDPRAPYLPGEVKFEG+PPPP A WLTP+DID YAD FS TGFTGGLNYYRAFDRTWEL
Sbjct: 181 NRDPRAPYLPGEVKFEGLPPPPPATWLTPEDIDVYADMFSRTGFTGGLNYYRAFDRTWEL 240

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFINQ 300
           TA WT A+IKVPVKFIVGDLDLTYHFPGAQ+YI+G  FK+DVPGLEEV+VMKDTSHFINQ
Sbjct: 241 TAAWTRAEIKVPVKFIVGDLDLTYHFPGAQEYIHGDGFKRDVPGLEEVVVMKDTSHFINQ 300

Query: 301 ERPHEINCYVHDFFNKFC 315
           ERP+E N ++HDFFNKFC
Sbjct: 301 ERPNETNAHIHDFFNKFC 316

BLAST of HG10019298 vs. ExPASy TrEMBL
Match: A0A6J1GAQ3 (uncharacterized protein LOC111452460 OS=Cucurbita moschata OX=3662 GN=LOC111452460 PE=4 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.2e-157
Identity = 254/318 (79.87%), Postives = 291/318 (91.51%), Query Frame = 0

Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+ I HTTV+TNGI LH+A+ G  TGPPVLLLHGFPE WYSWRHQ+++LSS+GYR IAPD
Sbjct: 1   MEAIQHTTVKTNGINLHIAAVG--TGPPVLLLHGFPEFWYSWRHQLIYLSSIGYRAIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPSA++YTTLHIVGDLVGLLDELGIEKVLLVGHDWGA+IAWY CLFRPD+I
Sbjct: 61  LRGYGDSDAPPSADAYTTLHIVGDLVGLLDELGIEKVLLVGHDWGAIIAWYLCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYM----EPGKAEEEFASVDIRELFKNVLS 180
           KA+VILSVQFFPRNPTTPF++ FK+VLGDQFYM    +PG+AEEEFA+VDI++ F +V+S
Sbjct: 121 KAAVILSVQFFPRNPTTPFVQAFKSVLGDQFYMVRFQQPGQAEEEFATVDIKKFFNDVMS 180

Query: 181 NRDPRAPYLPGEVKFEGVPPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRTWEL 240
           NRDPRAPYLPGEVKFEG+PPPP A WLTP+DID YADKFS TGFTGGLNYYRAF+RTWEL
Sbjct: 181 NRDPRAPYLPGEVKFEGLPPPPPATWLTPEDIDVYADKFSRTGFTGGLNYYRAFNRTWEL 240

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFINQ 300
           TA WT ++I+VPVK+IVGDLDLTYHFPGAQ+YI+G  FK+DVPGLEEV+VMKDTSHFINQ
Sbjct: 241 TAAWTKSEIRVPVKYIVGDLDLTYHFPGAQEYIHGDGFKRDVPGLEEVVVMKDTSHFINQ 300

Query: 301 ERPHEINCYVHDFFNKFC 315
           ERP+E N ++HDFF KFC
Sbjct: 301 ERPNETNAHIHDFFKKFC 316

BLAST of HG10019298 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 420.6 bits (1080), Expect = 1.1e-117
Identity = 188/318 (59.12%), Postives = 242/318 (76.10%), Query Frame = 0

Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+ I HTT+ TNGI +HVAS G  +GP +L +HGFP+LWYSWRHQ+V  +++GYR IAPD
Sbjct: 1   MEKIEHTTISTNGINMHVASIG--SGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120
           LRGYGDSDAPPS ESYT LHIVGDLVGLLD LG+++V LVGHDWGA++AW+ C+ RPD++
Sbjct: 61  LRGYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRV 120

Query: 121 KASVILSVQFFPRNPTTPFIEGFKAVLGDQFYM----EPGKAEEEFASVDIRELFKNVLS 180
            A V  SV F PRNP+   ++ F+A+ GD +Y+    EPG+ EE+FA VD ++L     +
Sbjct: 121 NALVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFT 180

Query: 181 NRDPRAPYLPGEVKFEGVPPPPLAP-WLTPQDIDFYADKFSHTGFTGGLNYYRAFDRTWE 240
           +R+PR P +P  V F G+P PP  P WLT QD+ FY DKFS  GFTGGLNYYRA + +WE
Sbjct: 181 SRNPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWE 240

Query: 241 LTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHFIN 300
           LTAPWTG QIKVPVKFIVGDLD+TY+ PG ++YI+    KK VP L+EV+VM+   HF++
Sbjct: 241 LTAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLH 300

Query: 301 QERPHEINCYVHDFFNKF 314
           QE+P E+  +++ FF KF
Sbjct: 301 QEKPDEVTDHIYGFFKKF 316

BLAST of HG10019298 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 377.9 bits (969), Expect = 8.1e-105
Identity = 176/323 (54.49%), Postives = 220/323 (68.11%), Query Frame = 0

Query: 1   MDTIHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPD 60
           M+ I H  V  NGI +H+A  GP  GP VLLLHGFP+LWY+WRHQI  LSS+GYR +APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGYGDSDAPPSAESYTTLHIVGDLVGLLDEL--GIEKVLLVGHDWGALIAWYFCLFRPD 120
           LRGYGDSD+P S   YT L++VGDLV LLD +    EKV LVGHDWGA+I W+ CLFRP+
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 KIKASVILSVQFFPRNPTTPFIEGFKAVLGDQFYM----EPGKAEEEFASVDIRELFKNV 180
           KI   V LSV +  RNP    ++GFKAV GD +Y+    EPGK E E AS D R   +N+
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 LSNRDPRAPYLPGEVKFEGVPPP-----PLAPWLTPQDIDFYADKFSHTGFTGGLNYYRA 240
            + R    P LP +  F   P P      L  W + +D+DFY  KF   GFTGGLNYYRA
Sbjct: 181 FTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYRA 240

Query: 241 FDRTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKD 300
            D  WELTAPWTGA+I+VPVKF+ GD D+ Y  PG ++YI+G  F  DVP L+E++V++D
Sbjct: 241 MDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIED 300

Query: 301 TSHFINQERPHEINCYVHDFFNK 313
             HF+NQE+P E+  +++DFF K
Sbjct: 301 AGHFVNQEKPQEVTAHINDFFTK 323

BLAST of HG10019298 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 345.5 bits (885), Expect = 4.5e-95
Identity = 162/324 (50.00%), Postives = 221/324 (68.21%), Query Frame = 0

Query: 1   MDTIHHTTVQTNGIKLHVA---SAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVI 60
           +D + H T++ NGI +HVA    +G    P +L LHGFPELWY+WRHQ+V LSS+GYR I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGYGDSDAPPSAESYTTLHIVGDLVGLLDEL--GIEKVLLVGHDWGALIAWYFCLF 120
           APDLRGYGD++AP   E YT L++ GD+V L+D +  G + V +VGHDWGA+IAW  C +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RPDKIKASVILSVQFFPRNPTTPFIEGFKAVLGDQFYM----EPGKAEEEFASVDIRELF 180
           RP+K+KA V +SV F PRNP    +   + V GD +Y+    + G+ E EF  +    + 
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 KNVLSNRDPRAPYLPGEVKFEGV--PPPPLAPWLTPQDIDFYADKFSHTGFTGGLNYYRA 240
           K  L+ + P    LP +  F+        L  WLT +D+D+Y  K+ + GFTG +NYYR 
Sbjct: 231 KEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYRN 290

Query: 241 FDRTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKD 300
            DR WELTAPWTGA+I+VPVKFI+GD DLTY+FPGA++YING  FK+DVP L+E +V+K 
Sbjct: 291 IDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKG 350

Query: 301 TSHFINQERPHEINCYVHDFFNKF 314
             HF+++E P  IN ++H+FF+KF
Sbjct: 351 LGHFLHEENPDVINQHIHNFFHKF 374

BLAST of HG10019298 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 334.3 bits (856), Expect = 1.0e-91
Identity = 163/321 (50.78%), Postives = 212/321 (66.04%), Query Frame = 0

Query: 4   IHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPDLRG 63
           + H  V+ NGI +HVA  GP  GP VLLLHGFPELWYSWRHQI  L++ GYR +APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  YGDSDAPPSAESYTTLHIVGDLVGLLDELGI---EKVLLVGHDWGALIAWYFCLFRPDKI 123
           YGDSDAP    SYT  +IVGDL+ ++  L     EKV +VGHDWGALIAWY CLFRPD++
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 KASVILSV--QFFPRNPTTPFIEGFKAVLGDQFYM----EPGKAEEEFASVDIRELFKNV 183
           KA V LSV   F P +P+   ++  +A  GD +Y+    E G  E E A V    + K +
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 LSNRDPRAPYLPGEVKFEGVPPP--PLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDR 243
           L+ R P    +P +  F G      PL  WLT +D+ ++  KF   GF+G +NYYR F+R
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFEEKGFSGPVNYYRNFNR 240

Query: 244 TWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSH 303
             EL  PW G++I+VP KF++G+LDL Y+ PG ++YI+G  FK+DVP LEE +VM+  +H
Sbjct: 241 NNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGVAH 300

Query: 304 FINQERPHEINCYVHDFFNKF 314
           FINQE+P EI   + DF +KF
Sbjct: 301 FINQEKPQEILQIILDFISKF 321

BLAST of HG10019298 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 327.4 bits (838), Expect = 1.3e-89
Identity = 160/320 (50.00%), Postives = 211/320 (65.94%), Query Frame = 0

Query: 4   IHHTTVQTNGIKLHVASAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSVGYRVIAPDLRG 63
           + H  V+ NGI +HVA  GP  G  VLLLHGFPELWYSWRHQI  L++ GYR +APDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 64  YGDSDAPPSAESYTTLHIVGDLVGLLDELGIE--KVLLVGHDWGALIAWYFCLFRPDKIK 123
           YGDSDAP    S+T  +IVGDLV ++  L  E  KV +VGHDWGALIAWY CLFRPDK+K
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 124 ASVILSV--QFFPRNPTTPFIEGFKAVLGDQFYM----EPGKAEEEFASVDIRELFKNVL 183
           A V LSV   F+P +P+   ++  +AV G+ +Y+    E G  E E A V    + K +L
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 184 SNRDPRAPYLPGEVKFEGVPPP--PLAPWLTPQDIDFYADKFSHTGFTGGLNYYRAFDRT 243
           + R P    +P +  F G      PL  WLT +D+ ++  KF   GF G +NYYR F+R 
Sbjct: 181 TYRTPGPLIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFKEKGFCGPVNYYRNFNRN 240

Query: 244 WELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQKYINGHAFKKDVPGLEEVIVMKDTSHF 303
            EL  PW G++I+VP KF++G+LDL Y+ PG ++YI+G  FK+DVP +EE +VM+  +HF
Sbjct: 241 NELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVAHF 300

Query: 304 INQERPHEINCYVHDFFNKF 314
           +NQE+P EI   + DF + F
Sbjct: 301 LNQEKPQEILQIILDFISTF 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038905173.17.4e-17792.14epoxide hydrolase A-like [Benincasa hispida][more]
XP_004152243.15.7e-16987.42uncharacterized protein LOC101204575 [Cucumis sativus] >KGN52824.1 hypothetical ... [more]
KAA0044388.11.8e-16285.22bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa] >TYK29516.1 bif... [more]
XP_008454324.21.8e-16285.22PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo][more]
XP_022998019.12.0e-15881.13uncharacterized protein LOC111492796 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
I6YGS01.4e-5638.22Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
Q6Q2C23.6e-4936.21Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
P349132.0e-4733.56Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
P349147.8e-4432.69Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
P802998.6e-4331.19Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0KV842.8e-16987.42AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001... [more]
A0A5A7TNE28.6e-16385.22Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BXU78.6e-16385.22bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494759 PE... [more]
A0A6J1KD679.9e-15981.13uncharacterized protein LOC111492796 OS=Cucurbita maxima OX=3661 GN=LOC111492796... [more]
A0A6J1GAQ33.2e-15779.87uncharacterized protein LOC111452460 OS=Cucurbita moschata OX=3662 GN=LOC1114524... [more]
Match NameE-valueIdentityDescription
AT4G02340.11.1e-11759.12alpha/beta-Hydrolases superfamily protein [more]
AT3G05600.18.1e-10554.49alpha/beta-Hydrolases superfamily protein [more]
AT4G15960.14.5e-9550.00alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.11.0e-9150.78soluble epoxide hydrolase [more]
AT2G26750.11.3e-8950.00alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 113..126
score: 16.01
coord: 99..112
score: 33.25
coord: 53..68
score: 53.23
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 27..136
e-value: 3.8E-24
score: 85.7
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 53..68
score: 64.77
coord: 99..112
score: 55.52
coord: 33..51
score: 58.85
coord: 287..309
score: 33.99
coord: 113..126
score: 42.53
coord: 247..263
score: 38.77
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..313
e-value: 2.1E-102
score: 345.2
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 3..312
NoneNo IPR availablePANTHERPTHR43329:SF58OS05G0273800 PROTEINcoord: 1..313
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 1..313

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10019298.1HG10019298.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity