HG10020201 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10020201 vs. NCBI nr
Match: XP_008442735.1 (PREDICTED: phosphatidylinositol 4-phosphate 5-kinase 9 [Cucumis melo] >KAA0043970.1 phosphatidylinositol 4-phosphate 5-kinase 9 [Cucumis melo var. makuwa] >TYK25170.1 phosphatidylinositol 4-phosphate 5-kinase 9 [Cucumis melo var. makuwa])

HSP 1 Score: 1632.8 bits (4227), Expect = 0.0e+00
Identity = 804/832 (96.63%), Postives = 816/832 (98.08%), Query Frame = 0

Query: 1   MSGPEAIVENVEGALSSAERTKSLDAISIKDFASAITNGETVHTSESARFRVGELLLVNG 60
           MSGPEAIVENV+GALSSAERTKSLDAISIKDF SAITNGETVH+SESARFRVGELLLVNG
Sbjct: 1   MSGPEAIVENVDGALSSAERTKSLDAISIKDFTSAITNGETVHSSESARFRVGELLLVNG 60

Query: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEFSGGYMHG 120
           ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMR G GKIRFPSGAVYEGEFSGGYMHG
Sbjct: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRHGNGKIRFPSGAVYEGEFSGGYMHG 120

Query: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG 180
           TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG
Sbjct: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG 180

Query: 181 NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLKDGKGSFY 240
           NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVG WT GLKDGKGSFY
Sbjct: 181 NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGTWTRGLKDGKGSFY 240

Query: 241 PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDK 300
           PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDK
Sbjct: 241 PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDK 300

Query: 301 ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTKIPILER 360
           ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHD SSGLSEYE GGNEIDTKIPILER
Sbjct: 301 ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDPSSGLSEYEFGGNEIDTKIPILER 360

Query: 361 EYMQGVLISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420
           EYMQGVLISELV+DNSFTSPSS+RAKR+QRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI
Sbjct: 361 EYMQGVLISELVLDNSFTSPSSRRAKRKQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420

Query: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480
           RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR
Sbjct: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480

Query: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540
           EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD
Sbjct: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540

Query: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600
           YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA
Sbjct: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600

Query: 601 DKVEIDENTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP 660
           DKVEIDENTILKDLDLNYSFYLEPTWR ALLKQIEIDSKFLE QNIMDYSLLLGVHYRAP
Sbjct: 601 DKVEIDENTILKDLDLNYSFYLEPTWREALLKQIEIDSKFLEEQNIMDYSLLLGVHYRAP 660

Query: 661 QQLQSHISYSRSMRADGLGILAEDDPFEDDMSTYPQGLVLVPRTDDNSVVVGPHIRGSRL 720
           QQLQS++SYSRSMRADGLGILAE+DPFEDD+STYPQGLVLVPRTDDNSVVVGPHIRGSRL
Sbjct: 661 QQLQSNMSYSRSMRADGLGILAEEDPFEDDISTYPQGLVLVPRTDDNSVVVGPHIRGSRL 720

Query: 721 R---AYGDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780
           R   A GDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI
Sbjct: 721 RASSATGDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780

Query: 781 LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSERFLDFIFQKVFPQNALAS 830
           LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYS+RFL+FI  +VFPQNA AS
Sbjct: 781 LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSKRFLEFIISRVFPQNARAS 832

BLAST of HG10020201 vs. NCBI nr
Match: XP_004137849.1 (phosphatidylinositol 4-phosphate 5-kinase 9 [Cucumis sativus] >KGN59008.1 hypothetical protein Csa_001635 [Cucumis sativus])

HSP 1 Score: 1629.4 bits (4218), Expect = 0.0e+00
Identity = 801/832 (96.27%), Postives = 815/832 (97.96%), Query Frame = 0

Query: 1   MSGPEAIVENVEGALSSAERTKSLDAISIKDFASAITNGETVHTSESARFRVGELLLVNG 60
           MSGPEAIVENVEGAL+SAERTKSLDAISIKDF SAITNGETVH+SESARFRVGELLLVNG
Sbjct: 1   MSGPEAIVENVEGALTSAERTKSLDAISIKDFTSAITNGETVHSSESARFRVGELLLVNG 60

Query: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEFSGGYMHG 120
           ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMR G GKIRFPSGAVYEGEFSGGYMHG
Sbjct: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRHGNGKIRFPSGAVYEGEFSGGYMHG 120

Query: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG 180
           TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG
Sbjct: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG 180

Query: 181 NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLKDGKGSFY 240
           NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVG WT GLKDGKGSFY
Sbjct: 181 NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGTWTRGLKDGKGSFY 240

Query: 241 PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDK 300
           PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVG+NQQSSRVSSDK
Sbjct: 241 PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGQNQQSSRVSSDK 300

Query: 301 ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTKIPILER 360
           ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHD SSG SEYELG NEIDTKIPILER
Sbjct: 301 ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDPSSGFSEYELGRNEIDTKIPILER 360

Query: 361 EYMQGVLISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420
           EYMQGVLISELV++NSFTSPSS+RAKR+QRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI
Sbjct: 361 EYMQGVLISELVLENSFTSPSSRRAKRKQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420

Query: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480
           RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR
Sbjct: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480

Query: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540
           EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD
Sbjct: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540

Query: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600
           YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA
Sbjct: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600

Query: 601 DKVEIDENTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP 660
           DKVEIDENTILKDLDLNYSF+LEPTWR ALLKQIEIDSKFLE QNIMDYSLLLGVHYRAP
Sbjct: 601 DKVEIDENTILKDLDLNYSFFLEPTWREALLKQIEIDSKFLEEQNIMDYSLLLGVHYRAP 660

Query: 661 QQLQSHISYSRSMRADGLGILAEDDPFEDDMSTYPQGLVLVPRTDDNSVVVGPHIRGSRL 720
           QQLQSHISYSRSMRADGLGILAEDDPFEDD+STYPQGLVLVPRTDDNSV+VGPHIRGSRL
Sbjct: 661 QQLQSHISYSRSMRADGLGILAEDDPFEDDISTYPQGLVLVPRTDDNSVIVGPHIRGSRL 720

Query: 721 R---AYGDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780
           R   A GDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI
Sbjct: 721 RASSATGDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780

Query: 781 LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSERFLDFIFQKVFPQNALAS 830
           LQEYNMSKKIEHAYKS+QFDSLSISAVDPTFYS+RFL+FI  +VFPQNA AS
Sbjct: 781 LQEYNMSKKIEHAYKSMQFDSLSISAVDPTFYSKRFLEFIISRVFPQNARAS 832

BLAST of HG10020201 vs. NCBI nr
Match: XP_038905936.1 (phosphatidylinositol 4-phosphate 5-kinase 9 [Benincasa hispida] >XP_038905937.1 phosphatidylinositol 4-phosphate 5-kinase 9 [Benincasa hispida])

HSP 1 Score: 1624.4 bits (4205), Expect = 0.0e+00
Identity = 802/831 (96.51%), Postives = 812/831 (97.71%), Query Frame = 0

Query: 1   MSGPEAIVENVEGALSSAERTKSLDAISIKDFASAITNGETVHTSESARFRVGELLLVNG 60
           MSGPE IVENVEGALSSAERTKSLDAIS+KDF SAITNGETVHTSESA FRVGELLLVNG
Sbjct: 1   MSGPETIVENVEGALSSAERTKSLDAISVKDFTSAITNGETVHTSESAGFRVGELLLVNG 60

Query: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEFSGGYMHG 120
           ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMR G GKIRFPSGAVY+GEFSGGYMHG
Sbjct: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRHGNGKIRFPSGAVYDGEFSGGYMHG 120

Query: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG 180
           TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGD FEGSWMQG PEGPGKYTWANGNVYLG
Sbjct: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDSFEGSWMQGIPEGPGKYTWANGNVYLG 180

Query: 181 NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLKDGKGSFY 240
           NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVG WT GLKDGKGSFY
Sbjct: 181 NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGTWTRGLKDGKGSFY 240

Query: 241 PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDK 300
           PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSR SSDK
Sbjct: 241 PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRGSSDK 300

Query: 301 ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTKIPILER 360
           ISKG+LLNLEQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTKIPILER
Sbjct: 301 ISKGSLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTKIPILER 360

Query: 361 EYMQGVLISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420
           EYMQGVLISELVIDNSF+SPSS+RAKRRQRKLVKE KKPGEMIIKGHRSYDLMLSLQLGI
Sbjct: 361 EYMQGVLISELVIDNSFSSPSSRRAKRRQRKLVKEGKKPGEMIIKGHRSYDLMLSLQLGI 420

Query: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480
           RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR
Sbjct: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480

Query: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540
           EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD
Sbjct: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540

Query: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600
           YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA
Sbjct: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600

Query: 601 DKVEIDENTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP 660
           DKVEIDENTILKDLDLNYSFYLEPTWR ALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP
Sbjct: 601 DKVEIDENTILKDLDLNYSFYLEPTWREALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP 660

Query: 661 QQLQSHISYSRSMRADGLGILAEDDPFEDDMSTYPQGLVLVPRTDDNSVVVGPHIRGSRL 720
           QQLQSHISYSRSMRADGLGILAEDD FE+D+STYPQGLVLVPRTDDNSVVVGPHIRGSRL
Sbjct: 661 QQLQSHISYSRSMRADGLGILAEDDTFEEDISTYPQGLVLVPRTDDNSVVVGPHIRGSRL 720

Query: 721 R---AYGDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780
           R   A GDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI
Sbjct: 721 RASSASGDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780

Query: 781 LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSERFLDFIFQKVFPQNALA 829
           LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYS+RFL+FI Q+VFPQNA A
Sbjct: 781 LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSQRFLEFINQRVFPQNARA 831

BLAST of HG10020201 vs. NCBI nr
Match: XP_022144637.1 (phosphatidylinositol 4-phosphate 5-kinase 9 [Momordica charantia])

HSP 1 Score: 1604.3 bits (4153), Expect = 0.0e+00
Identity = 788/832 (94.71%), Postives = 809/832 (97.24%), Query Frame = 0

Query: 1   MSGPEAIVENVEGALSSAERTKSLDAISIKDFASAITNGETVHTSESARFRVGELLLVNG 60
           MSGPEAIVENVEGALSSAERTKSLDAIS KDF SAITNGE VHTSESARFRVGELLL+NG
Sbjct: 1   MSGPEAIVENVEGALSSAERTKSLDAISSKDFTSAITNGEAVHTSESARFRVGELLLLNG 60

Query: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEFSGGYMHG 120
           ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQG G+IRFPSGAVYEGEFSGGYMHG
Sbjct: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGNGRIRFPSGAVYEGEFSGGYMHG 120

Query: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG 180
           TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGD+FEGSWMQG PEGPGKYTW NGN+YLG
Sbjct: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDIFEGSWMQGIPEGPGKYTWTNGNIYLG 180

Query: 181 NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLKDGKGSFY 240
           NMKAG MSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVG WT GLKDGKGSFY
Sbjct: 181 NMKAGKMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGTWTRGLKDGKGSFY 240

Query: 241 PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDK 300
           PKGSR+ AVQEIYLKALRKRGLLPDL+ +NHAHIHHATSGDMGNFKVGENQQSSRVSSDK
Sbjct: 241 PKGSRITAVQEIYLKALRKRGLLPDLRKQNHAHIHHATSGDMGNFKVGENQQSSRVSSDK 300

Query: 301 ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTKIPILER 360
           +SKGNLLNLEQYR+KNVSLERRWSLEVSIEKVLGHDSSSGLSEY+LGG+E+D KIPILER
Sbjct: 301 LSKGNLLNLEQYRSKNVSLERRWSLEVSIEKVLGHDSSSGLSEYDLGGHEVDAKIPILER 360

Query: 361 EYMQGVLISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420
           EYMQGVLISELVIDNSF+SPSS+RAKR+Q+KLVKEVKKPGEMIIKGHRSYDLMLSLQLGI
Sbjct: 361 EYMQGVLISELVIDNSFSSPSSRRAKRKQKKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420

Query: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480
           RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR
Sbjct: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480

Query: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540
           EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD
Sbjct: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540

Query: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600
           YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA
Sbjct: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600

Query: 601 DKVEIDENTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP 660
           DKVEIDENTILKDLDLNY+FYLEPTWR ALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP
Sbjct: 601 DKVEIDENTILKDLDLNYTFYLEPTWRVALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP 660

Query: 661 QQLQSHISYSRSMRADGLGILAEDDPFEDDMSTYPQGLVLVPRTDDNSVVVGPHIRGSRL 720
           Q LQSHISY+RSMRADGLGILAEDDPFE+D+S YPQGLVLVPR DDNSVVVGPHIRGSRL
Sbjct: 661 QHLQSHISYNRSMRADGLGILAEDDPFEEDISNYPQGLVLVPRIDDNSVVVGPHIRGSRL 720

Query: 721 R---AYGDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780
           R   A G EEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI
Sbjct: 721 RASSAAGGEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780

Query: 781 LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSERFLDFIFQKVFPQNALAS 830
           LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYS RFL+FI QKVFPQNALAS
Sbjct: 781 LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSHRFLEFI-QKVFPQNALAS 831

BLAST of HG10020201 vs. NCBI nr
Match: XP_022934963.1 (phosphatidylinositol 4-phosphate 5-kinase 9-like [Cucurbita moschata] >XP_022934973.1 phosphatidylinositol 4-phosphate 5-kinase 9-like [Cucurbita moschata])

HSP 1 Score: 1567.7 bits (4058), Expect = 0.0e+00
Identity = 768/831 (92.42%), Postives = 797/831 (95.91%), Query Frame = 0

Query: 1   MSGPEAIVENVEGALSSAERTKSLDAISIKDFASAITNGETVHTSESARFRVGELLLVNG 60
           MSGP AIV+NVEG LSSAERTKSLDAI+ K+F SAI NGETVHTSESARFRVGELLLVNG
Sbjct: 1   MSGPVAIVDNVEGGLSSAERTKSLDAITSKEFTSAIINGETVHTSESARFRVGELLLVNG 60

Query: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEFSGGYMHG 120
           ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMR G GKIRFPSGAVYEGEFSGGYMHG
Sbjct: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRHGNGKIRFPSGAVYEGEFSGGYMHG 120

Query: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG 180
           TGTY GPDNL YKGRWKLNLKHGLGYQVYPNGD+FEGSWMQGTPEGPGKYTW NGNVYLG
Sbjct: 121 TGTYTGPDNLVYKGRWKLNLKHGLGYQVYPNGDIFEGSWMQGTPEGPGKYTWVNGNVYLG 180

Query: 181 NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLKDGKGSFY 240
           NMKAG MSGKGTLTWINGDSFEGNWL GMMHGFGVYMWSDGGCYVG WT GLKDGKGSFY
Sbjct: 181 NMKAGAMSGKGTLTWINGDSFEGNWLSGMMHGFGVYMWSDGGCYVGTWTRGLKDGKGSFY 240

Query: 241 PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDK 300
           PKGSRLPA QEIYLKALRK+GLLPD + +NHAHIHHA SGDMGNFKVGENQQSSRV+SDK
Sbjct: 241 PKGSRLPAGQEIYLKALRKKGLLPDSRKQNHAHIHHAISGDMGNFKVGENQQSSRVTSDK 300

Query: 301 ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTKIPILER 360
           +SKG+LLNLEQYR+KNVSLERRWSLEVSIEKVLGHDSSSGLS+YE+GGN +DTKIPILER
Sbjct: 301 LSKGSLLNLEQYRSKNVSLERRWSLEVSIEKVLGHDSSSGLSDYEMGGNVVDTKIPILER 360

Query: 361 EYMQGVLISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420
           EYMQGVLISELVIDNSF+SPSS+RA+RRQ+KLVKEV KPGEMIIKGHRSYDLMLSLQLGI
Sbjct: 361 EYMQGVLISELVIDNSFSSPSSRRARRRQKKLVKEVNKPGEMIIKGHRSYDLMLSLQLGI 420

Query: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480
           RYTVGKITP+QRREVRSSDFGPRASFWM FPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR
Sbjct: 421 RYTVGKITPVQRREVRSSDFGPRASFWMTFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480

Query: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540
           EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD
Sbjct: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540

Query: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600
           YHHHVRSYENTLITKFFGLHRIKP+SGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA
Sbjct: 541 YHHHVRSYENTLITKFFGLHRIKPASGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600

Query: 601 DKVEIDENTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP 660
           DKVEIDENTILKDLDLNYSFYLEPTWR ALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP
Sbjct: 601 DKVEIDENTILKDLDLNYSFYLEPTWREALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP 660

Query: 661 QQLQSHISYSRSMRADGLGILAEDDPFEDDMSTYPQGLVLVPRTDDNSVVVGPHIRGSRL 720
           QQ QSH+SY+RSMRADGLGILAEDDPFE D+STYPQGLVLVPR DD+SVVVG HIRGSRL
Sbjct: 661 QQPQSHLSYNRSMRADGLGILAEDDPFE-DISTYPQGLVLVPRIDDDSVVVGSHIRGSRL 720

Query: 721 R---AYGDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780
           R   A GD EVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI
Sbjct: 721 RASSAAGDGEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780

Query: 781 LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSERFLDFIFQKVFPQNALA 829
           LQEYNMSKKIEHAYKS+QFDS+SIS+VDPTFYS+RFL FI Q+VFPQNA A
Sbjct: 781 LQEYNMSKKIEHAYKSLQFDSISISSVDPTFYSQRFLQFIIQRVFPQNARA 830

BLAST of HG10020201 vs. ExPASy Swiss-Prot
Match: Q8L850 (Phosphatidylinositol 4-phosphate 5-kinase 9 OS=Arabidopsis thaliana OX=3702 GN=PIP5K9 PE=1 SV=2)

HSP 1 Score: 1248.0 bits (3228), Expect = 0.0e+00
Identity = 620/822 (75.43%), Postives = 703/822 (85.52%), Query Frame = 0

Query: 10  NVEGALSSAERTKSLDAISIKDFASAITNGETVHTSESARFRVGELLLVNGELYSGSLLG 69
           +V GA+S AERTKS+DA++ K+  SA+ +GE   TSE ARFRV EL+L +GE YSGSLLG
Sbjct: 5   DVRGAVSFAERTKSVDALTKKEILSALNSGEVSETSEDARFRVRELVLPDGESYSGSLLG 64

Query: 70  NIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEFSGGYMHGTGTYIGPDN 129
           N+PEG GKY+WSDGC+Y+GEWRRGMR G G +R+ SGA Y+GEFSGGYMHG+GTY+  + 
Sbjct: 65  NVPEGPGKYIWSDGCVYDGEWRRGMRHGIGNMRWASGASYDGEFSGGYMHGSGTYVDANK 124

Query: 130 LTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLGNMKAGGMSG 189
           LTYKGRW+LNLKHGLGYQVYPNGDVFEGSW+QG  EGPGKYTWAN NVYLG+MK G MSG
Sbjct: 125 LTYKGRWRLNLKHGLGYQVYPNGDVFEGSWIQGLGEGPGKYTWANKNVYLGDMKGGKMSG 184

Query: 190 KGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLKDGKGSFYPKGSRLPAV 249
           KGTLTW+ GDS+EG+WL GMMHG GVY WSDGGCYVG WT GLKDGKGSFY  G+R+P V
Sbjct: 185 KGTLTWVTGDSYEGSWLNGMMHGVGVYTWSDGGCYVGTWTRGLKDGKGSFYSAGTRVPVV 244

Query: 250 QEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDKISKGNLLNL 309
           QE YL ALRKRG+LPD++ +N      A+S +M N +VG N+       +K+SKG+L+NL
Sbjct: 245 QEFYLNALRKRGVLPDMRRQNQV----ASSVNMENLRVGVNR-------NKLSKGSLINL 304

Query: 310 EQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTK--IPILEREYMQGVL 369
           EQ R   VSLERRWSLEVSIEKV+GH  S   +     G+ +  K  IPILEREYMQGVL
Sbjct: 305 EQSRNGRVSLERRWSLEVSIEKVIGHGYSDLSTAVLDSGSSVQYKANIPILEREYMQGVL 364

Query: 370 ISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGIRYTVGKI 429
           ISELV++N F S +S+RAKR+ ++LVKE KKPGE++IKGHRSYDLMLSLQLGIRYTVGKI
Sbjct: 365 ISELVVNNGF-SRTSRRAKRKHKRLVKEAKKPGEVVIKGHRSYDLMLSLQLGIRYTVGKI 424

Query: 430 TPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLREMFKIDA 489
           TPIQRR+VR++DFGPRASFWM FP+ GS +TPPH SEDFKWKDYCPMVFRNLREMFKIDA
Sbjct: 425 TPIQRRQVRTADFGPRASFWMTFPRAGSTMTPPHHSEDFKWKDYCPMVFRNLREMFKIDA 484

Query: 490 ADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPDYHHHVRS 549
           ADYMMSICGND LRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPDYHHHV++
Sbjct: 485 ADYMMSICGNDTLRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPDYHHHVKT 544

Query: 550 YENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSADKVEIDE 609
           YENTLITKFFGLHRIKPSSGQKFRFVVMGNMF T+LRIHRRFDLKGSSLGRSADKVEIDE
Sbjct: 545 YENTLITKFFGLHRIKPSSGQKFRFVVMGNMFFTDLRIHRRFDLKGSSLGRSADKVEIDE 604

Query: 610 NTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAPQQLQSHI 669
           NTILKDLDLNYSF+LE +WR  LL+Q+EIDSKFLE QNIMDYSLLLGVH+RAPQ L+S +
Sbjct: 605 NTILKDLDLNYSFFLETSWREGLLRQLEIDSKFLEAQNIMDYSLLLGVHHRAPQHLRSQL 664

Query: 670 SYSRSMRADGLGILAEDDPFEDDMSTYPQGLVLVPRTDDNSVVVGPHIRGSRLR--AYGD 729
             S+S+  D L  +AEDD  EDDM +Y +GLVLVPR  +N+ V GPHIRGSRLR  A GD
Sbjct: 665 VRSQSITTDALESVAEDDTIEDDMLSYHEGLVLVPRGSENT-VTGPHIRGSRLRASAVGD 724

Query: 730 EEVDLLLPGTARLQIQLGVNMPARAEQIPGKE--EKQMFHEAYDVVLYLGIIDILQEYNM 789
           EEVDLLLPGTARLQIQ GVNMPARAE IPG+E  EKQ+ H+  DVVLYLGIIDILQEYNM
Sbjct: 725 EEVDLLLPGTARLQIQQGVNMPARAELIPGREDKEKQILHDCCDVVLYLGIIDILQEYNM 784

Query: 790 SKKIEHAYKSIQFDSLSISAVDPTFYSERFLDFIFQKVFPQN 826
           +KKIEHAYKS+ FDSLSISAVDPTFYS+RFL+FI +KVFPQN
Sbjct: 785 TKKIEHAYKSLHFDSLSISAVDPTFYSQRFLEFI-KKVFPQN 812

BLAST of HG10020201 vs. ExPASy Swiss-Prot
Match: Q8RY89 (Phosphatidylinositol 4-phosphate 5-kinase 8 OS=Arabidopsis thaliana OX=3702 GN=PIP5K8 PE=1 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 1.6e-221
Identity = 412/797 (51.69%), Postives = 525/797 (65.87%), Query Frame = 0

Query: 51  RVGELLLVNGELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYE 110
           R  E +  NG++YSG L G +P G GKY W DG IYEG+W  G   G GK+ + SGA YE
Sbjct: 4   RPAERVFSNGDVYSGQLKGTLPHGKGKYAWPDGIIYEGDWEEGKISGRGKLMWSSGAKYE 63

Query: 111 GEFSGGYMHGTGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKY 170
           G+FSGGY+HG GT   PD   Y G W++N++HGLG + Y N DV++GSW +G  +G G Y
Sbjct: 64  GDFSGGYLHGFGTLTSPDGSVYAGAWRMNVRHGLGRKEYCNSDVYDGSWREGLQDGSGSY 123

Query: 171 TWANGNVYLGNMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTS 230
           +W NGN ++GN K G MSG+G ++W NGD F G WL G+ HG GVY ++DGG Y G W+ 
Sbjct: 124 SWYNGNRFIGNWKKGKMSGRGVMSWANGDLFNGFWLNGLRHGSGVYKYADGGFYFGTWSR 183

Query: 231 GLKDGKGSFYPKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGEN 290
           GLKDG G FYP GS+ P+++                  K H H  +    D GNF +  N
Sbjct: 184 GLKDGSGVFYPAGSKHPSLK------------------KWHRHFGY---DDTGNFLLSHN 243

Query: 291 QQSSRVSSDKISKGNLLNLEQY-------RTKNVSLERRWSLEVSIEKVLGH-DSSSGLS 350
             +  +   + SK    +L +        RT     +  WS        + H  SS    
Sbjct: 244 -STINIDDLRTSKAVSRSLSELTTTSGLTRTSERYPDDYWSTSDPPRDFMHHGPSSKSAR 303

Query: 351 EYELGGNEIDTKIPIL-EREYMQGVLISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPG- 410
             + G +EI  K PI+ EREYMQGVLI E ++     S      + R   L KEV     
Sbjct: 304 SVDSGQSEIRDKNPIVFEREYMQGVLIKERIM-----SSIDMSHRARPLNLTKEVTVSAC 363

Query: 411 EMIIKGHRSYDLMLSLQLGIRYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPP 470
              + G  ++ LML+LQLGIRYTVGKITP+  REVR+SDF  RA   M FP+ GSQ TPP
Sbjct: 364 VSFLGGKWNHYLMLNLQLGIRYTVGKITPVPPREVRASDFSERARIMMFFPRNGSQYTPP 423

Query: 471 HQSEDFKWKDYCPMVFRNLREMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDR 530
           H+S DF WKDYCPMVFRNLREMFK+DAADYMMSICG+D LRE+SSPGKSGS+F+LS DDR
Sbjct: 424 HKSIDFDWKDYCPMVFRNLREMFKLDAADYMMSICGDDGLREISSPGKSGSIFYLSHDDR 483

Query: 531 FMIKTLRKSEVKVLLRMLPDYHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFC 590
           F+IKTL++SE+KVLLRMLP Y+ HV  YENTLITKFFG+HRIK   G+K RFVVMGNMFC
Sbjct: 484 FVIKTLKRSELKVLLRMLPRYYEHVGDYENTLITKFFGVHRIKLKWGKKVRFVVMGNMFC 543

Query: 591 TELRIHRRFDLKGSSLGRSADKVEIDENTILKDLDLNYSFYLEPTWRAALLKQIEIDSKF 650
           TEL+IHRR+DLKGS+ GR  +K +I E T LKDLDL Y F+++   R AL KQI +D  F
Sbjct: 544 TELKIHRRYDLKGSTQGRYTEKNKIGEKTTLKDLDLAYEFHMDKLLREALFKQIILDCSF 603

Query: 651 LEVQNIMDYSLLLGVHYRAPQQLQSHISYSRSM---RADGLGILAEDDPFEDDMSTYPQG 710
           LE   I+DYSLLLG+H+RAP  L   +     M    +D +G +  + P E   S  P+G
Sbjct: 604 LESLQILDYSLLLGLHFRAPDPLTDILEPPNEMSDQESDSVGSVDVNLPREP--SIPPKG 663

Query: 711 LVLVPRTDDNSV--VVGPHIRGSRLRAY--GDEEVDLLLPGTARLQIQLGVNMPARAEQI 770
           L++V   + NSV    GPHIRGS LRA+  G++EVDL+LPGTARL++QLGVNMPA+A   
Sbjct: 664 LLMVTH-EPNSVNTAPGPHIRGSTLRAFSVGEKEVDLILPGTARLRVQLGVNMPAQAHHK 723

Query: 771 PGKEEKQM----FHEAYDVVLYLGIIDILQEYNMSKKIEHAYKSIQFDSLSISAVDPTFY 827
            G++ ++       E YDVV+Y+GIIDILQEYNM KK+EH  KS+++D ++ISA++PT Y
Sbjct: 724 LGQDNEESGTVELFEVYDVVVYMGIIDILQEYNMKKKVEHTCKSMKYDPMTISAIEPTLY 769

BLAST of HG10020201 vs. ExPASy Swiss-Prot
Match: Q6EX42 (Phosphatidylinositol 4-phosphate 5-kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PIPK1 PE=2 SV=2)

HSP 1 Score: 763.8 bits (1971), Expect = 2.0e-219
Identity = 410/799 (51.31%), Postives = 524/799 (65.58%), Query Frame = 0

Query: 57  LVNGELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEFSGG 116
           L NG++Y GS  G +P G GKY+W+DG +Y+GEW +    G G I++PSGA YEG+F GG
Sbjct: 35  LPNGDIYVGSFDGLVPHGPGKYMWTDGALYDGEWDKSKMTGRGLIQWPSGASYEGDFRGG 94

Query: 117 YMHGTGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGN 176
           ++ G GT+ G D   YKG W++N KHG+G  VY N D +EG W +G P+  GKYTWA+GN
Sbjct: 95  FIDGAGTFKGVDGSVYKGSWRMNKKHGMGTMVYSNSDTYEGFWNEGLPDEFGKYTWADGN 154

Query: 177 VYLGNMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLKDGK 236
           VY+G  K+G M+G G + WINGD+ + NWL G+ HG G   ++ G CY+G W  GLKDG 
Sbjct: 155 VYIGRWKSGKMNGSGVMQWINGDTLDCNWLNGLAHGKGYCKYASGACYIGTWDRGLKDGH 214

Query: 237 GSFYPKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRV 296
           G+FY  GS++P                 +L+  +    H  TS             +S  
Sbjct: 215 GTFYQPGSKIPC----------------NLEVSDCLTSHDGTS-------------ASSS 274

Query: 297 SSDKISKGNLLNLEQYRTKNVSLER--RWSLEVSIEKVLGHDSSSG-------------L 356
           S++KI+ G LL L Q   KN  L R       +S       D +SG              
Sbjct: 275 SNEKITIG-LLFLLQKLCKNWRLRRFLHRPRRISNGTTPVFDDNSGSHLCQDVSSKSFSA 334

Query: 357 SEYELGGNEIDTKIPILEREYMQGVLISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGE 416
            +  L  +E+D K  + EREY+QGVLI E     +  S  S+    ++    K+ K P E
Sbjct: 335 DDQCLQDSEVD-KDSVYEREYVQGVLIMEQ--PKNEDSRMSESGIAQENNWEKQAKGPME 394

Query: 417 MIIKGHRSYDLMLSLQLGIRYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPH 476
            I KGHRSY LML+LQLGIRYTVGKITP+  REVRS+DFGPRA   M FP EGSQ TPPH
Sbjct: 395 TIYKGHRSYYLMLNLQLGIRYTVGKITPVPLREVRSNDFGPRARIKMYFPCEGSQYTPPH 454

Query: 477 QSEDFKWKDYCPMVFRNLREMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRF 536
            S DF WKDYCPMVFRNLREMF IDAADYMMSICG D+L+ELSSPGKSGS+F+LSQD+RF
Sbjct: 455 YSVDFFWKDYCPMVFRNLREMFHIDAADYMMSICGGDSLKELSSPGKSGSIFYLSQDERF 514

Query: 537 MIKTLRKSEVK---------VLLRMLPDYHHHVRSYENTLITKFFGLHRIKPSSGQKFRF 596
           +IKTLRK+E+K         +LL+MLP Y++HV++Y+NTLITKFFG+HRI    G+K RF
Sbjct: 515 VIKTLRKTELKIGLMKYVLQILLKMLPKYYNHVKAYDNTLITKFFGVHRITLKPGRKVRF 574

Query: 597 VVMGNMFCTELRIHRRFDLKGSSLGRSADKVEIDENTILKDLDLNYSFYLEPTWRAALLK 656
           VVMGNMFCTELRIHR++DLKGS+ GRS  K  I+ENT LKDLDL+Y F+++  WR AL +
Sbjct: 575 VVMGNMFCTELRIHRKYDLKGSTQGRSTKKQNINENTTLKDLDLSYVFHVDKPWREALFR 634

Query: 657 QIEIDSKFLEVQNIMDYSLLLGVHYRAPQQLQSHISYSRSMRADGLGILAE-DDPFEDDM 716
           QI +D  FLE Q+I+DYS+LLG+H+RAP  L+   S   ++ + G+    E       + 
Sbjct: 635 QIALDCMFLESQSIIDYSMLLGIHFRAPNHLKRITSCQNALESTGISAETECSVALHHEE 694

Query: 717 STYPQGLVLVPRTDDNSVVVGPHIRGSRLRAY--GDEEVDLLLPGTARLQIQLGVNMPAR 776
           +   +G +LV   +    V G HIRGS +RA   G EEVDL+LPGT R ++QLGVNMPAR
Sbjct: 695 TISSKGFLLVAADEPGPAVRGSHIRGSMVRAAEGGYEEVDLVLPGTGRFRVQLGVNMPAR 754

Query: 777 A----EQIPGKEEKQMFHEAYDVVLYLGIIDILQEYNMSKKIEHAYKSIQFDSLSISAVD 825
           A    E +  + E +   E YDVVLYLGIIDILQEYN+SK++EHA KS++FD LSISAVD
Sbjct: 755 ARKVQEDVNVEVENRDTIEEYDVVLYLGIIDILQEYNVSKRVEHAVKSLKFDPLSISAVD 799

BLAST of HG10020201 vs. ExPASy Swiss-Prot
Match: Q9SUI2 (Phosphatidylinositol 4-phosphate 5-kinase 7 OS=Arabidopsis thaliana OX=3702 GN=PIP5K7 PE=1 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 2.3e-212
Identity = 397/789 (50.32%), Postives = 520/789 (65.91%), Query Frame = 0

Query: 51  RVGELLLVNGELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYE 110
           R G+    NG+ YSG + G IP G GKY WSDG IYEG+W  G   G GK+ + SGA YE
Sbjct: 4   RSGDREFPNGDFYSGEVKGIIPNGKGKYAWSDGTIYEGDWDEGKISGKGKLIWSSGAKYE 63

Query: 111 GEFSGGYMHGTGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKY 170
           G+FSGGY+HG GT   PD   Y G W++N++HGLG + Y N D+++G W +G  +G G Y
Sbjct: 64  GDFSGGYLHGFGTMTSPDESVYSGAWRMNVRHGLGRKEYCNSDLYDGLWKEGLQDGRGSY 123

Query: 171 TWANGNVYLGNMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTS 230
           +W NGN Y+GN K G M  +G + W NGD ++G WL G  HG GVY ++DG  Y G W+ 
Sbjct: 124 SWTNGNRYIGNWKKGKMCERGVMRWENGDLYDGFWLNGFRHGSGVYKFADGCLYYGTWSR 183

Query: 231 GLKDGKGSFYPKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGEN 290
           GLKDGKG FYP G++ P++++ + ++L                       D G F +   
Sbjct: 184 GLKDGKGVFYPAGTKQPSLKK-WCRSLE--------------------YDDTGKFVL--- 243

Query: 291 QQSSRVSSDKISKGNLLNLEQYRTKNVSLE------RRWSLEVSIEKVLGHDSSSGLSEY 350
            +S+ V+ +++   N +  +    K  + E      R ++    + K     S SG SE 
Sbjct: 244 SRSASVNVEELRSLNTVT-QSLSVKTSAGETTCDPPRDFTCHGPVSK-SARFSGSGQSEG 303

Query: 351 ELGGNEIDTKIPILEREYMQGVLISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGEM-I 410
           +      D    + EREYMQGVLI E ++ +   S   K   R      +EV+    M  
Sbjct: 304 Q------DKNRIVYEREYMQGVLIRETIMSSVDRSHKIKPPNR-----PREVRARSLMTF 363

Query: 411 IKGHRSYDLMLSLQLGIRYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQS 470
           ++G  +Y LML+LQLGIRYTVGKITP+ RREVR+SDFG  A   M FP++GS  TPPH+S
Sbjct: 364 LRGEHNYYLMLNLQLGIRYTVGKITPVPRREVRASDFGKNARTKMFFPRDGSNFTPPHKS 423

Query: 471 EDFKWKDYCPMVFRNLREMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMI 530
            DF WKDYCPMVFRNLR+MFK+DAA+YMMSICG+D L E+SSPGKSGS+F+LS DDRF+I
Sbjct: 424 VDFSWKDYCPMVFRNLRQMFKLDAAEYMMSICGDDGLTEISSPGKSGSIFYLSHDDRFVI 483

Query: 531 KTLRKSEVKVLLRMLPDYHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTEL 590
           KTL+KSE++VLLRMLP Y+ HV  +ENTLITKFFG+HRI    G+K RFVVMGNMFCTEL
Sbjct: 484 KTLKKSELQVLLRMLPKYYEHVGDHENTLITKFFGVHRITLKWGKKVRFVVMGNMFCTEL 543

Query: 591 RIHRRFDLKGSSLGRSADKVEIDENTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEV 650
           +IHRR+DLKGS+ GR  +K++I E T LKDLDL Y F+++   R AL KQI +D  FLE 
Sbjct: 544 KIHRRYDLKGSTQGRFTEKIKIQEKTTLKDLDLAYEFHMDKLLREALFKQIYLDCSFLES 603

Query: 651 QNIMDYSLLLGVHYRAPQQLQSHISYSRSMRADGLGILAEDDPFEDDMSTYPQGLVLVPR 710
            NI+DYSLLLG+H+RAP QL   +    +M +D   + + D     + S  P+GL+LV  
Sbjct: 604 LNIIDYSLLLGLHFRAPGQLNDILEPPNAM-SDQESVSSVDVGLTQEHSIPPKGLLLVTH 663

Query: 711 TDDNSV--VVGPHIRGSRLRAY--GDEEVDLLLPGTARLQIQLGVNMPARAEQ--IPGKE 770
            + NSV    GPHIRGS LRA+  G++EVDL+LPGTARL++QLGVNMPA+A    I  KE
Sbjct: 664 -EPNSVNTAPGPHIRGSTLRAFSVGEQEVDLILPGTARLRVQLGVNMPAQAHHKLIEDKE 723

Query: 771 EKQMFH--EAYDVVLYLGIIDILQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSERFLD 825
           E       E YDVV+Y+GIIDILQEYN  KK+EH  KS+Q+D ++IS  +P+ YS+RF++
Sbjct: 724 ESATIELFEVYDVVVYMGIIDILQEYNTKKKVEHTCKSLQYDPMTISVTEPSTYSKRFVN 752

BLAST of HG10020201 vs. ExPASy Swiss-Prot
Match: Q8L796 (Phosphatidylinositol 4-phosphate 5-kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PIP5K2 PE=1 SV=2)

HSP 1 Score: 620.5 bits (1599), Expect = 2.7e-176
Identity = 344/773 (44.50%), Postives = 439/773 (56.79%), Query Frame = 0

Query: 57  LVNGELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEFSGG 116
           L NG+LY G+  G  P G+GKY+W DGC+YEGEW+RG   G GK  +PSGA YEGEF  G
Sbjct: 76  LPNGDLYMGTFSGGFPNGSGKYLWKDGCMYEGEWKRGKASGKGKFSWPSGATYEGEFKSG 135

Query: 117 YMHGTGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGN 176
            M G+GT++G D  TY+G W  + K G G + Y NGD +EG+W +   +G G+Y W NGN
Sbjct: 136 RMEGSGTFVGVDGDTYRGSWVADRKQGHGQKRYANGDYYEGTWRRNLQDGRGRYVWMNGN 195

Query: 177 VYLGNMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLKDGK 236
            Y G  + G + GKG L W NG+ +EG W  G+  G GV+ W+DG  ++G W        
Sbjct: 196 QYTGEWRNGVICGKGVLAWPNGNRYEGQWENGVPKGSGVFTWADGSSWIGSW-------- 255

Query: 237 GSFYPKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRV 296
                                                  + +S  M NF           
Sbjct: 256 ---------------------------------------NESSNLMRNF----------- 315

Query: 297 SSDKISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTKIP 356
             D I K  L+         V+  +R S++     + G      +  +E  G   D    
Sbjct: 316 -FDGIEKNELI---------VATRKRSSVDSGAGSLTGEKIFPRICIWESDGEAGDITCD 375

Query: 357 ILEREYMQGVLISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGEMIIKGHRSYDLMLSL 416
           I++      +    + ID        ++ ++       E KKPGE I KGH+ YDLML+L
Sbjct: 376 IVDNVEASVIYRDRISIDKD----GFRQFRKNPCCFSGEAKKPGETISKGHKKYDLMLNL 435

Query: 417 QLGIRYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVF 476
           Q GIRY+VGK   +  R+++ SDF P   FW  FP EGS+ TPPH S DF+WKDYCP+VF
Sbjct: 436 QHGIRYSVGKHASVV-RDLKQSDFDPSEKFWTRFPPEGSKTTPPHLSVDFRWKDYCPLVF 495

Query: 477 RNLREMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLR 536
           R LRE+F +D ADYM++ICGNDALRELSSPGKSGS F+L+QDDRFMIKT++KSEVKVLLR
Sbjct: 496 RRLRELFTVDPADYMLAICGNDALRELSSPGKSGSFFYLTQDDRFMIKTVKKSEVKVLLR 555

Query: 537 MLPDYHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSL 596
           MLP Y+ HV  YENTL+T+F+G+H IKP  GQK RF+VMGN+FC+E RI RRFDLKGSS 
Sbjct: 556 MLPSYYKHVCQYENTLVTRFYGVHCIKPVGGQKTRFIVMGNLFCSEYRIQRRFDLKGSSH 615

Query: 597 GRSADKV--EIDENTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEVQNIMDYSLLLG 656
           GR   K   EIDE T LKDLDLN++F L+  W   L+ QI+ D +FLE + IMDYSLL+G
Sbjct: 616 GRYTSKPEGEIDETTTLKDLDLNFAFRLQRNWYQELMTQIKRDCEFLEAERIMDYSLLVG 675

Query: 657 VHYRAPQQLQSHISYSRSMRADGLGILAEDDPFEDDMSTYPQGLVLVPRTDDNSVVVGPH 716
           VH+R                         DD   D M   P    ++      S      
Sbjct: 676 VHFR-------------------------DDNTGDKMGLSP---FVLRSGKIESYQSEKF 735

Query: 717 IRGSRLRAYGDEEVDLLLPGTARLQIQLGVNMPARAEQI----------PGKEEKQMFHE 776
           +RG R      +++D +L G   L I+LG NMPARAE++           G    Q   E
Sbjct: 736 MRGCRFLEAELQDMDRILAGRKPL-IRLGANMPARAERMARRSDYDQYSSGGTNYQSHGE 746

Query: 777 AYDVVLYLGIIDILQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSERFLDFI 818
            Y+VVLY GIIDILQ+Y++SKKIEHAYKS+Q D  SISAVDP  YS RF DFI
Sbjct: 796 VYEVVLYFGIIDILQDYDISKKIEHAYKSLQADPASISAVDPKLYSRRFRDFI 746

BLAST of HG10020201 vs. ExPASy TrEMBL
Match: A0A5D3DPG7 (1-phosphatidylinositol-4-phosphate 5-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002960 PE=4 SV=1)

HSP 1 Score: 1632.8 bits (4227), Expect = 0.0e+00
Identity = 804/832 (96.63%), Postives = 816/832 (98.08%), Query Frame = 0

Query: 1   MSGPEAIVENVEGALSSAERTKSLDAISIKDFASAITNGETVHTSESARFRVGELLLVNG 60
           MSGPEAIVENV+GALSSAERTKSLDAISIKDF SAITNGETVH+SESARFRVGELLLVNG
Sbjct: 1   MSGPEAIVENVDGALSSAERTKSLDAISIKDFTSAITNGETVHSSESARFRVGELLLVNG 60

Query: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEFSGGYMHG 120
           ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMR G GKIRFPSGAVYEGEFSGGYMHG
Sbjct: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRHGNGKIRFPSGAVYEGEFSGGYMHG 120

Query: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG 180
           TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG
Sbjct: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG 180

Query: 181 NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLKDGKGSFY 240
           NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVG WT GLKDGKGSFY
Sbjct: 181 NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGTWTRGLKDGKGSFY 240

Query: 241 PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDK 300
           PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDK
Sbjct: 241 PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDK 300

Query: 301 ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTKIPILER 360
           ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHD SSGLSEYE GGNEIDTKIPILER
Sbjct: 301 ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDPSSGLSEYEFGGNEIDTKIPILER 360

Query: 361 EYMQGVLISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420
           EYMQGVLISELV+DNSFTSPSS+RAKR+QRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI
Sbjct: 361 EYMQGVLISELVLDNSFTSPSSRRAKRKQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420

Query: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480
           RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR
Sbjct: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480

Query: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540
           EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD
Sbjct: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540

Query: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600
           YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA
Sbjct: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600

Query: 601 DKVEIDENTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP 660
           DKVEIDENTILKDLDLNYSFYLEPTWR ALLKQIEIDSKFLE QNIMDYSLLLGVHYRAP
Sbjct: 601 DKVEIDENTILKDLDLNYSFYLEPTWREALLKQIEIDSKFLEEQNIMDYSLLLGVHYRAP 660

Query: 661 QQLQSHISYSRSMRADGLGILAEDDPFEDDMSTYPQGLVLVPRTDDNSVVVGPHIRGSRL 720
           QQLQS++SYSRSMRADGLGILAE+DPFEDD+STYPQGLVLVPRTDDNSVVVGPHIRGSRL
Sbjct: 661 QQLQSNMSYSRSMRADGLGILAEEDPFEDDISTYPQGLVLVPRTDDNSVVVGPHIRGSRL 720

Query: 721 R---AYGDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780
           R   A GDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI
Sbjct: 721 RASSATGDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780

Query: 781 LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSERFLDFIFQKVFPQNALAS 830
           LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYS+RFL+FI  +VFPQNA AS
Sbjct: 781 LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSKRFLEFIISRVFPQNARAS 832

BLAST of HG10020201 vs. ExPASy TrEMBL
Match: A0A1S3B5X1 (1-phosphatidylinositol-4-phosphate 5-kinase OS=Cucumis melo OX=3656 GN=LOC103486523 PE=4 SV=1)

HSP 1 Score: 1632.8 bits (4227), Expect = 0.0e+00
Identity = 804/832 (96.63%), Postives = 816/832 (98.08%), Query Frame = 0

Query: 1   MSGPEAIVENVEGALSSAERTKSLDAISIKDFASAITNGETVHTSESARFRVGELLLVNG 60
           MSGPEAIVENV+GALSSAERTKSLDAISIKDF SAITNGETVH+SESARFRVGELLLVNG
Sbjct: 1   MSGPEAIVENVDGALSSAERTKSLDAISIKDFTSAITNGETVHSSESARFRVGELLLVNG 60

Query: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEFSGGYMHG 120
           ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMR G GKIRFPSGAVYEGEFSGGYMHG
Sbjct: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRHGNGKIRFPSGAVYEGEFSGGYMHG 120

Query: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG 180
           TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG
Sbjct: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG 180

Query: 181 NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLKDGKGSFY 240
           NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVG WT GLKDGKGSFY
Sbjct: 181 NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGTWTRGLKDGKGSFY 240

Query: 241 PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDK 300
           PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDK
Sbjct: 241 PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDK 300

Query: 301 ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTKIPILER 360
           ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHD SSGLSEYE GGNEIDTKIPILER
Sbjct: 301 ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDPSSGLSEYEFGGNEIDTKIPILER 360

Query: 361 EYMQGVLISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420
           EYMQGVLISELV+DNSFTSPSS+RAKR+QRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI
Sbjct: 361 EYMQGVLISELVLDNSFTSPSSRRAKRKQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420

Query: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480
           RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR
Sbjct: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480

Query: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540
           EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD
Sbjct: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540

Query: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600
           YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA
Sbjct: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600

Query: 601 DKVEIDENTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP 660
           DKVEIDENTILKDLDLNYSFYLEPTWR ALLKQIEIDSKFLE QNIMDYSLLLGVHYRAP
Sbjct: 601 DKVEIDENTILKDLDLNYSFYLEPTWREALLKQIEIDSKFLEEQNIMDYSLLLGVHYRAP 660

Query: 661 QQLQSHISYSRSMRADGLGILAEDDPFEDDMSTYPQGLVLVPRTDDNSVVVGPHIRGSRL 720
           QQLQS++SYSRSMRADGLGILAE+DPFEDD+STYPQGLVLVPRTDDNSVVVGPHIRGSRL
Sbjct: 661 QQLQSNMSYSRSMRADGLGILAEEDPFEDDISTYPQGLVLVPRTDDNSVVVGPHIRGSRL 720

Query: 721 R---AYGDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780
           R   A GDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI
Sbjct: 721 RASSATGDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780

Query: 781 LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSERFLDFIFQKVFPQNALAS 830
           LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYS+RFL+FI  +VFPQNA AS
Sbjct: 781 LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSKRFLEFIISRVFPQNARAS 832

BLAST of HG10020201 vs. ExPASy TrEMBL
Match: A0A0A0LB60 (1-phosphatidylinositol-4-phosphate 5-kinase OS=Cucumis sativus OX=3659 GN=Csa_3G743450 PE=4 SV=1)

HSP 1 Score: 1629.4 bits (4218), Expect = 0.0e+00
Identity = 801/832 (96.27%), Postives = 815/832 (97.96%), Query Frame = 0

Query: 1   MSGPEAIVENVEGALSSAERTKSLDAISIKDFASAITNGETVHTSESARFRVGELLLVNG 60
           MSGPEAIVENVEGAL+SAERTKSLDAISIKDF SAITNGETVH+SESARFRVGELLLVNG
Sbjct: 1   MSGPEAIVENVEGALTSAERTKSLDAISIKDFTSAITNGETVHSSESARFRVGELLLVNG 60

Query: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEFSGGYMHG 120
           ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMR G GKIRFPSGAVYEGEFSGGYMHG
Sbjct: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRHGNGKIRFPSGAVYEGEFSGGYMHG 120

Query: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG 180
           TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG
Sbjct: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG 180

Query: 181 NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLKDGKGSFY 240
           NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVG WT GLKDGKGSFY
Sbjct: 181 NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGTWTRGLKDGKGSFY 240

Query: 241 PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDK 300
           PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVG+NQQSSRVSSDK
Sbjct: 241 PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGQNQQSSRVSSDK 300

Query: 301 ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTKIPILER 360
           ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHD SSG SEYELG NEIDTKIPILER
Sbjct: 301 ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDPSSGFSEYELGRNEIDTKIPILER 360

Query: 361 EYMQGVLISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420
           EYMQGVLISELV++NSFTSPSS+RAKR+QRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI
Sbjct: 361 EYMQGVLISELVLENSFTSPSSRRAKRKQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420

Query: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480
           RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR
Sbjct: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480

Query: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540
           EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD
Sbjct: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540

Query: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600
           YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA
Sbjct: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600

Query: 601 DKVEIDENTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP 660
           DKVEIDENTILKDLDLNYSF+LEPTWR ALLKQIEIDSKFLE QNIMDYSLLLGVHYRAP
Sbjct: 601 DKVEIDENTILKDLDLNYSFFLEPTWREALLKQIEIDSKFLEEQNIMDYSLLLGVHYRAP 660

Query: 661 QQLQSHISYSRSMRADGLGILAEDDPFEDDMSTYPQGLVLVPRTDDNSVVVGPHIRGSRL 720
           QQLQSHISYSRSMRADGLGILAEDDPFEDD+STYPQGLVLVPRTDDNSV+VGPHIRGSRL
Sbjct: 661 QQLQSHISYSRSMRADGLGILAEDDPFEDDISTYPQGLVLVPRTDDNSVIVGPHIRGSRL 720

Query: 721 R---AYGDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780
           R   A GDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI
Sbjct: 721 RASSATGDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780

Query: 781 LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSERFLDFIFQKVFPQNALAS 830
           LQEYNMSKKIEHAYKS+QFDSLSISAVDPTFYS+RFL+FI  +VFPQNA AS
Sbjct: 781 LQEYNMSKKIEHAYKSMQFDSLSISAVDPTFYSKRFLEFIISRVFPQNARAS 832

BLAST of HG10020201 vs. ExPASy TrEMBL
Match: A0A6J1CU82 (1-phosphatidylinositol-4-phosphate 5-kinase OS=Momordica charantia OX=3673 GN=LOC111014279 PE=4 SV=1)

HSP 1 Score: 1604.3 bits (4153), Expect = 0.0e+00
Identity = 788/832 (94.71%), Postives = 809/832 (97.24%), Query Frame = 0

Query: 1   MSGPEAIVENVEGALSSAERTKSLDAISIKDFASAITNGETVHTSESARFRVGELLLVNG 60
           MSGPEAIVENVEGALSSAERTKSLDAIS KDF SAITNGE VHTSESARFRVGELLL+NG
Sbjct: 1   MSGPEAIVENVEGALSSAERTKSLDAISSKDFTSAITNGEAVHTSESARFRVGELLLLNG 60

Query: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEFSGGYMHG 120
           ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQG G+IRFPSGAVYEGEFSGGYMHG
Sbjct: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGNGRIRFPSGAVYEGEFSGGYMHG 120

Query: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG 180
           TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGD+FEGSWMQG PEGPGKYTW NGN+YLG
Sbjct: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDIFEGSWMQGIPEGPGKYTWTNGNIYLG 180

Query: 181 NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLKDGKGSFY 240
           NMKAG MSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVG WT GLKDGKGSFY
Sbjct: 181 NMKAGKMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGTWTRGLKDGKGSFY 240

Query: 241 PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDK 300
           PKGSR+ AVQEIYLKALRKRGLLPDL+ +NHAHIHHATSGDMGNFKVGENQQSSRVSSDK
Sbjct: 241 PKGSRITAVQEIYLKALRKRGLLPDLRKQNHAHIHHATSGDMGNFKVGENQQSSRVSSDK 300

Query: 301 ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTKIPILER 360
           +SKGNLLNLEQYR+KNVSLERRWSLEVSIEKVLGHDSSSGLSEY+LGG+E+D KIPILER
Sbjct: 301 LSKGNLLNLEQYRSKNVSLERRWSLEVSIEKVLGHDSSSGLSEYDLGGHEVDAKIPILER 360

Query: 361 EYMQGVLISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420
           EYMQGVLISELVIDNSF+SPSS+RAKR+Q+KLVKEVKKPGEMIIKGHRSYDLMLSLQLGI
Sbjct: 361 EYMQGVLISELVIDNSFSSPSSRRAKRKQKKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420

Query: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480
           RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR
Sbjct: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480

Query: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540
           EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD
Sbjct: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540

Query: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600
           YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA
Sbjct: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600

Query: 601 DKVEIDENTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP 660
           DKVEIDENTILKDLDLNY+FYLEPTWR ALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP
Sbjct: 601 DKVEIDENTILKDLDLNYTFYLEPTWRVALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP 660

Query: 661 QQLQSHISYSRSMRADGLGILAEDDPFEDDMSTYPQGLVLVPRTDDNSVVVGPHIRGSRL 720
           Q LQSHISY+RSMRADGLGILAEDDPFE+D+S YPQGLVLVPR DDNSVVVGPHIRGSRL
Sbjct: 661 QHLQSHISYNRSMRADGLGILAEDDPFEEDISNYPQGLVLVPRIDDNSVVVGPHIRGSRL 720

Query: 721 R---AYGDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780
           R   A G EEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI
Sbjct: 721 RASSAAGGEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780

Query: 781 LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSERFLDFIFQKVFPQNALAS 830
           LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYS RFL+FI QKVFPQNALAS
Sbjct: 781 LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSHRFLEFI-QKVFPQNALAS 831

BLAST of HG10020201 vs. ExPASy TrEMBL
Match: A0A6J1F969 (1-phosphatidylinositol-4-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111441972 PE=4 SV=1)

HSP 1 Score: 1567.7 bits (4058), Expect = 0.0e+00
Identity = 768/831 (92.42%), Postives = 797/831 (95.91%), Query Frame = 0

Query: 1   MSGPEAIVENVEGALSSAERTKSLDAISIKDFASAITNGETVHTSESARFRVGELLLVNG 60
           MSGP AIV+NVEG LSSAERTKSLDAI+ K+F SAI NGETVHTSESARFRVGELLLVNG
Sbjct: 1   MSGPVAIVDNVEGGLSSAERTKSLDAITSKEFTSAIINGETVHTSESARFRVGELLLVNG 60

Query: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEFSGGYMHG 120
           ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMR G GKIRFPSGAVYEGEFSGGYMHG
Sbjct: 61  ELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRHGNGKIRFPSGAVYEGEFSGGYMHG 120

Query: 121 TGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLG 180
           TGTY GPDNL YKGRWKLNLKHGLGYQVYPNGD+FEGSWMQGTPEGPGKYTW NGNVYLG
Sbjct: 121 TGTYTGPDNLVYKGRWKLNLKHGLGYQVYPNGDIFEGSWMQGTPEGPGKYTWVNGNVYLG 180

Query: 181 NMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLKDGKGSFY 240
           NMKAG MSGKGTLTWINGDSFEGNWL GMMHGFGVYMWSDGGCYVG WT GLKDGKGSFY
Sbjct: 181 NMKAGAMSGKGTLTWINGDSFEGNWLSGMMHGFGVYMWSDGGCYVGTWTRGLKDGKGSFY 240

Query: 241 PKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDK 300
           PKGSRLPA QEIYLKALRK+GLLPD + +NHAHIHHA SGDMGNFKVGENQQSSRV+SDK
Sbjct: 241 PKGSRLPAGQEIYLKALRKKGLLPDSRKQNHAHIHHAISGDMGNFKVGENQQSSRVTSDK 300

Query: 301 ISKGNLLNLEQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTKIPILER 360
           +SKG+LLNLEQYR+KNVSLERRWSLEVSIEKVLGHDSSSGLS+YE+GGN +DTKIPILER
Sbjct: 301 LSKGSLLNLEQYRSKNVSLERRWSLEVSIEKVLGHDSSSGLSDYEMGGNVVDTKIPILER 360

Query: 361 EYMQGVLISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGI 420
           EYMQGVLISELVIDNSF+SPSS+RA+RRQ+KLVKEV KPGEMIIKGHRSYDLMLSLQLGI
Sbjct: 361 EYMQGVLISELVIDNSFSSPSSRRARRRQKKLVKEVNKPGEMIIKGHRSYDLMLSLQLGI 420

Query: 421 RYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480
           RYTVGKITP+QRREVRSSDFGPRASFWM FPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR
Sbjct: 421 RYTVGKITPVQRREVRSSDFGPRASFWMTFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLR 480

Query: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540
           EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD
Sbjct: 481 EMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPD 540

Query: 541 YHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600
           YHHHVRSYENTLITKFFGLHRIKP+SGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA
Sbjct: 541 YHHHVRSYENTLITKFFGLHRIKPASGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSA 600

Query: 601 DKVEIDENTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP 660
           DKVEIDENTILKDLDLNYSFYLEPTWR ALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP
Sbjct: 601 DKVEIDENTILKDLDLNYSFYLEPTWREALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAP 660

Query: 661 QQLQSHISYSRSMRADGLGILAEDDPFEDDMSTYPQGLVLVPRTDDNSVVVGPHIRGSRL 720
           QQ QSH+SY+RSMRADGLGILAEDDPFE D+STYPQGLVLVPR DD+SVVVG HIRGSRL
Sbjct: 661 QQPQSHLSYNRSMRADGLGILAEDDPFE-DISTYPQGLVLVPRIDDDSVVVGSHIRGSRL 720

Query: 721 R---AYGDEEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780
           R   A GD EVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI
Sbjct: 721 RASSAAGDGEVDLLLPGTARLQIQLGVNMPARAEQIPGKEEKQMFHEAYDVVLYLGIIDI 780

Query: 781 LQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSERFLDFIFQKVFPQNALA 829
           LQEYNMSKKIEHAYKS+QFDS+SIS+VDPTFYS+RFL FI Q+VFPQNA A
Sbjct: 781 LQEYNMSKKIEHAYKSLQFDSISISSVDPTFYSQRFLQFIIQRVFPQNARA 830

BLAST of HG10020201 vs. TAIR 10
Match: AT3G09920.1 (phosphatidyl inositol monophosphate 5 kinase )

HSP 1 Score: 1248.0 bits (3228), Expect = 0.0e+00
Identity = 620/822 (75.43%), Postives = 703/822 (85.52%), Query Frame = 0

Query: 10  NVEGALSSAERTKSLDAISIKDFASAITNGETVHTSESARFRVGELLLVNGELYSGSLLG 69
           +V GA+S AERTKS+DA++ K+  SA+ +GE   TSE ARFRV EL+L +GE YSGSLLG
Sbjct: 5   DVRGAVSFAERTKSVDALTKKEILSALNSGEVSETSEDARFRVRELVLPDGESYSGSLLG 64

Query: 70  NIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEFSGGYMHGTGTYIGPDN 129
           N+PEG GKY+WSDGC+Y+GEWRRGMR G G +R+ SGA Y+GEFSGGYMHG+GTY+  + 
Sbjct: 65  NVPEGPGKYIWSDGCVYDGEWRRGMRHGIGNMRWASGASYDGEFSGGYMHGSGTYVDANK 124

Query: 130 LTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLGNMKAGGMSG 189
           LTYKGRW+LNLKHGLGYQVYPNGDVFEGSW+QG  EGPGKYTWAN NVYLG+MK G MSG
Sbjct: 125 LTYKGRWRLNLKHGLGYQVYPNGDVFEGSWIQGLGEGPGKYTWANKNVYLGDMKGGKMSG 184

Query: 190 KGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLKDGKGSFYPKGSRLPAV 249
           KGTLTW+ GDS+EG+WL GMMHG GVY WSDGGCYVG WT GLKDGKGSFY  G+R+P V
Sbjct: 185 KGTLTWVTGDSYEGSWLNGMMHGVGVYTWSDGGCYVGTWTRGLKDGKGSFYSAGTRVPVV 244

Query: 250 QEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDKISKGNLLNL 309
           QE YL ALRKRG+LPD++ +N      A+S +M N +VG N+       +K+SKG+L+NL
Sbjct: 245 QEFYLNALRKRGVLPDMRRQNQV----ASSVNMENLRVGVNR-------NKLSKGSLINL 304

Query: 310 EQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTK--IPILEREYMQGVL 369
           EQ R   VSLERRWSLEVSIEKV+GH  S   +     G+ +  K  IPILEREYMQGVL
Sbjct: 305 EQSRNGRVSLERRWSLEVSIEKVIGHGYSDLSTAVLDSGSSVQYKANIPILEREYMQGVL 364

Query: 370 ISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGIRYTVGKI 429
           ISELV++N F S +S+RAKR+ ++LVKE KKPGE++IKGHRSYDLMLSLQLGIRYTVGKI
Sbjct: 365 ISELVVNNGF-SRTSRRAKRKHKRLVKEAKKPGEVVIKGHRSYDLMLSLQLGIRYTVGKI 424

Query: 430 TPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLREMFKIDA 489
           TPIQRR+VR++DFGPRASFWM FP+ GS +TPPH SEDFKWKDYCPMVFRNLREMFKIDA
Sbjct: 425 TPIQRRQVRTADFGPRASFWMTFPRAGSTMTPPHHSEDFKWKDYCPMVFRNLREMFKIDA 484

Query: 490 ADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPDYHHHVRS 549
           ADYMMSICGND LRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPDYHHHV++
Sbjct: 485 ADYMMSICGNDTLRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPDYHHHVKT 544

Query: 550 YENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSADKVEIDE 609
           YENTLITKFFGLHRIKPSSGQKFRFVVMGNMF T+LRIHRRFDLKGSSLGRSADKVEIDE
Sbjct: 545 YENTLITKFFGLHRIKPSSGQKFRFVVMGNMFFTDLRIHRRFDLKGSSLGRSADKVEIDE 604

Query: 610 NTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAPQQLQSHI 669
           NTILKDLDLNYSF+LE +WR  LL+Q+EIDSKFLE QNIMDYSLLLGVH+RAPQ L+S +
Sbjct: 605 NTILKDLDLNYSFFLETSWREGLLRQLEIDSKFLEAQNIMDYSLLLGVHHRAPQHLRSQL 664

Query: 670 SYSRSMRADGLGILAEDDPFEDDMSTYPQGLVLVPRTDDNSVVVGPHIRGSRLR--AYGD 729
             S+S+  D L  +AEDD  EDDM +Y +GLVLVPR  +N+ V GPHIRGSRLR  A GD
Sbjct: 665 VRSQSITTDALESVAEDDTIEDDMLSYHEGLVLVPRGSENT-VTGPHIRGSRLRASAVGD 724

Query: 730 EEVDLLLPGTARLQIQLGVNMPARAEQIPGKE--EKQMFHEAYDVVLYLGIIDILQEYNM 789
           EEVDLLLPGTARLQIQ GVNMPARAE IPG+E  EKQ+ H+  DVVLYLGIIDILQEYNM
Sbjct: 725 EEVDLLLPGTARLQIQQGVNMPARAELIPGREDKEKQILHDCCDVVLYLGIIDILQEYNM 784

Query: 790 SKKIEHAYKSIQFDSLSISAVDPTFYSERFLDFIFQKVFPQN 826
           +KKIEHAYKS+ FDSLSISAVDPTFYS+RFL+FI +KVFPQN
Sbjct: 785 TKKIEHAYKSLHFDSLSISAVDPTFYSQRFLEFI-KKVFPQN 812

BLAST of HG10020201 vs. TAIR 10
Match: AT3G09920.2 (phosphatidyl inositol monophosphate 5 kinase )

HSP 1 Score: 1248.0 bits (3228), Expect = 0.0e+00
Identity = 620/822 (75.43%), Postives = 703/822 (85.52%), Query Frame = 0

Query: 10  NVEGALSSAERTKSLDAISIKDFASAITNGETVHTSESARFRVGELLLVNGELYSGSLLG 69
           +V GA+S AERTKS+DA++ K+  SA+ +GE   TSE ARFRV EL+L +GE YSGSLLG
Sbjct: 5   DVRGAVSFAERTKSVDALTKKEILSALNSGEVSETSEDARFRVRELVLPDGESYSGSLLG 64

Query: 70  NIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEFSGGYMHGTGTYIGPDN 129
           N+PEG GKY+WSDGC+Y+GEWRRGMR G G +R+ SGA Y+GEFSGGYMHG+GTY+  + 
Sbjct: 65  NVPEGPGKYIWSDGCVYDGEWRRGMRHGIGNMRWASGASYDGEFSGGYMHGSGTYVDANK 124

Query: 130 LTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLGNMKAGGMSG 189
           LTYKGRW+LNLKHGLGYQVYPNGDVFEGSW+QG  EGPGKYTWAN NVYLG+MK G MSG
Sbjct: 125 LTYKGRWRLNLKHGLGYQVYPNGDVFEGSWIQGLGEGPGKYTWANKNVYLGDMKGGKMSG 184

Query: 190 KGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLKDGKGSFYPKGSRLPAV 249
           KGTLTW+ GDS+EG+WL GMMHG GVY WSDGGCYVG WT GLKDGKGSFY  G+R+P V
Sbjct: 185 KGTLTWVTGDSYEGSWLNGMMHGVGVYTWSDGGCYVGTWTRGLKDGKGSFYSAGTRVPVV 244

Query: 250 QEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDKISKGNLLNL 309
           QE YL ALRKRG+LPD++ +N      A+S +M N +VG N+       +K+SKG+L+NL
Sbjct: 245 QEFYLNALRKRGVLPDMRRQNQV----ASSVNMENLRVGVNR-------NKLSKGSLINL 304

Query: 310 EQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTK--IPILEREYMQGVL 369
           EQ R   VSLERRWSLEVSIEKV+GH  S   +     G+ +  K  IPILEREYMQGVL
Sbjct: 305 EQSRNGRVSLERRWSLEVSIEKVIGHGYSDLSTAVLDSGSSVQYKANIPILEREYMQGVL 364

Query: 370 ISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGIRYTVGKI 429
           ISELV++N F S +S+RAKR+ ++LVKE KKPGE++IKGHRSYDLMLSLQLGIRYTVGKI
Sbjct: 365 ISELVVNNGF-SRTSRRAKRKHKRLVKEAKKPGEVVIKGHRSYDLMLSLQLGIRYTVGKI 424

Query: 430 TPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLREMFKIDA 489
           TPIQRR+VR++DFGPRASFWM FP+ GS +TPPH SEDFKWKDYCPMVFRNLREMFKIDA
Sbjct: 425 TPIQRRQVRTADFGPRASFWMTFPRAGSTMTPPHHSEDFKWKDYCPMVFRNLREMFKIDA 484

Query: 490 ADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPDYHHHVRS 549
           ADYMMSICGND LRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPDYHHHV++
Sbjct: 485 ADYMMSICGNDTLRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPDYHHHVKT 544

Query: 550 YENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSADKVEIDE 609
           YENTLITKFFGLHRIKPSSGQKFRFVVMGNMF T+LRIHRRFDLKGSSLGRSADKVEIDE
Sbjct: 545 YENTLITKFFGLHRIKPSSGQKFRFVVMGNMFFTDLRIHRRFDLKGSSLGRSADKVEIDE 604

Query: 610 NTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAPQQLQSHI 669
           NTILKDLDLNYSF+LE +WR  LL+Q+EIDSKFLE QNIMDYSLLLGVH+RAPQ L+S +
Sbjct: 605 NTILKDLDLNYSFFLETSWREGLLRQLEIDSKFLEAQNIMDYSLLLGVHHRAPQHLRSQL 664

Query: 670 SYSRSMRADGLGILAEDDPFEDDMSTYPQGLVLVPRTDDNSVVVGPHIRGSRLR--AYGD 729
             S+S+  D L  +AEDD  EDDM +Y +GLVLVPR  +N+ V GPHIRGSRLR  A GD
Sbjct: 665 VRSQSITTDALESVAEDDTIEDDMLSYHEGLVLVPRGSENT-VTGPHIRGSRLRASAVGD 724

Query: 730 EEVDLLLPGTARLQIQLGVNMPARAEQIPGKE--EKQMFHEAYDVVLYLGIIDILQEYNM 789
           EEVDLLLPGTARLQIQ GVNMPARAE IPG+E  EKQ+ H+  DVVLYLGIIDILQEYNM
Sbjct: 725 EEVDLLLPGTARLQIQQGVNMPARAELIPGREDKEKQILHDCCDVVLYLGIIDILQEYNM 784

Query: 790 SKKIEHAYKSIQFDSLSISAVDPTFYSERFLDFIFQKVFPQN 826
           +KKIEHAYKS+ FDSLSISAVDPTFYS+RFL+FI +KVFPQN
Sbjct: 785 TKKIEHAYKSLHFDSLSISAVDPTFYSQRFLEFI-KKVFPQN 812

BLAST of HG10020201 vs. TAIR 10
Match: AT3G09920.3 (phosphatidyl inositol monophosphate 5 kinase )

HSP 1 Score: 1248.0 bits (3228), Expect = 0.0e+00
Identity = 620/822 (75.43%), Postives = 703/822 (85.52%), Query Frame = 0

Query: 10  NVEGALSSAERTKSLDAISIKDFASAITNGETVHTSESARFRVGELLLVNGELYSGSLLG 69
           +V GA+S AERTKS+DA++ K+  SA+ +GE   TSE ARFRV EL+L +GE YSGSLLG
Sbjct: 5   DVRGAVSFAERTKSVDALTKKEILSALNSGEVSETSEDARFRVRELVLPDGESYSGSLLG 64

Query: 70  NIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEFSGGYMHGTGTYIGPDN 129
           N+PEG GKY+WSDGC+Y+GEWRRGMR G G +R+ SGA Y+GEFSGGYMHG+GTY+  + 
Sbjct: 65  NVPEGPGKYIWSDGCVYDGEWRRGMRHGIGNMRWASGASYDGEFSGGYMHGSGTYVDANK 124

Query: 130 LTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWANGNVYLGNMKAGGMSG 189
           LTYKGRW+LNLKHGLGYQVYPNGDVFEGSW+QG  EGPGKYTWAN NVYLG+MK G MSG
Sbjct: 125 LTYKGRWRLNLKHGLGYQVYPNGDVFEGSWIQGLGEGPGKYTWANKNVYLGDMKGGKMSG 184

Query: 190 KGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLKDGKGSFYPKGSRLPAV 249
           KGTLTW+ GDS+EG+WL GMMHG GVY WSDGGCYVG WT GLKDGKGSFY  G+R+P V
Sbjct: 185 KGTLTWVTGDSYEGSWLNGMMHGVGVYTWSDGGCYVGTWTRGLKDGKGSFYSAGTRVPVV 244

Query: 250 QEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQSSRVSSDKISKGNLLNL 309
           QE YL ALRKRG+LPD++ +N      A+S +M N +VG N+       +K+SKG+L+NL
Sbjct: 245 QEFYLNALRKRGVLPDMRRQNQV----ASSVNMENLRVGVNR-------NKLSKGSLINL 304

Query: 310 EQYRTKNVSLERRWSLEVSIEKVLGHDSSSGLSEYELGGNEIDTK--IPILEREYMQGVL 369
           EQ R   VSLERRWSLEVSIEKV+GH  S   +     G+ +  K  IPILEREYMQGVL
Sbjct: 305 EQSRNGRVSLERRWSLEVSIEKVIGHGYSDLSTAVLDSGSSVQYKANIPILEREYMQGVL 364

Query: 370 ISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPGEMIIKGHRSYDLMLSLQLGIRYTVGKI 429
           ISELV++N F S +S+RAKR+ ++LVKE KKPGE++IKGHRSYDLMLSLQLGIRYTVGKI
Sbjct: 365 ISELVVNNGF-SRTSRRAKRKHKRLVKEAKKPGEVVIKGHRSYDLMLSLQLGIRYTVGKI 424

Query: 430 TPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQSEDFKWKDYCPMVFRNLREMFKIDA 489
           TPIQRR+VR++DFGPRASFWM FP+ GS +TPPH SEDFKWKDYCPMVFRNLREMFKIDA
Sbjct: 425 TPIQRRQVRTADFGPRASFWMTFPRAGSTMTPPHHSEDFKWKDYCPMVFRNLREMFKIDA 484

Query: 490 ADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPDYHHHVRS 549
           ADYMMSICGND LRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPDYHHHV++
Sbjct: 485 ADYMMSICGNDTLRELSSPGKSGSVFFLSQDDRFMIKTLRKSEVKVLLRMLPDYHHHVKT 544

Query: 550 YENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTELRIHRRFDLKGSSLGRSADKVEIDE 609
           YENTLITKFFGLHRIKPSSGQKFRFVVMGNMF T+LRIHRRFDLKGSSLGRSADKVEIDE
Sbjct: 545 YENTLITKFFGLHRIKPSSGQKFRFVVMGNMFFTDLRIHRRFDLKGSSLGRSADKVEIDE 604

Query: 610 NTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEVQNIMDYSLLLGVHYRAPQQLQSHI 669
           NTILKDLDLNYSF+LE +WR  LL+Q+EIDSKFLE QNIMDYSLLLGVH+RAPQ L+S +
Sbjct: 605 NTILKDLDLNYSFFLETSWREGLLRQLEIDSKFLEAQNIMDYSLLLGVHHRAPQHLRSQL 664

Query: 670 SYSRSMRADGLGILAEDDPFEDDMSTYPQGLVLVPRTDDNSVVVGPHIRGSRLR--AYGD 729
             S+S+  D L  +AEDD  EDDM +Y +GLVLVPR  +N+ V GPHIRGSRLR  A GD
Sbjct: 665 VRSQSITTDALESVAEDDTIEDDMLSYHEGLVLVPRGSENT-VTGPHIRGSRLRASAVGD 724

Query: 730 EEVDLLLPGTARLQIQLGVNMPARAEQIPGKE--EKQMFHEAYDVVLYLGIIDILQEYNM 789
           EEVDLLLPGTARLQIQ GVNMPARAE IPG+E  EKQ+ H+  DVVLYLGIIDILQEYNM
Sbjct: 725 EEVDLLLPGTARLQIQQGVNMPARAELIPGREDKEKQILHDCCDVVLYLGIIDILQEYNM 784

Query: 790 SKKIEHAYKSIQFDSLSISAVDPTFYSERFLDFIFQKVFPQN 826
           +KKIEHAYKS+ FDSLSISAVDPTFYS+RFL+FI +KVFPQN
Sbjct: 785 TKKIEHAYKSLHFDSLSISAVDPTFYSQRFLEFI-KKVFPQN 812

BLAST of HG10020201 vs. TAIR 10
Match: AT1G60890.1 (Phosphatidylinositol-4-phosphate 5-kinase family protein )

HSP 1 Score: 770.8 bits (1989), Expect = 1.1e-222
Identity = 412/797 (51.69%), Postives = 525/797 (65.87%), Query Frame = 0

Query: 51  RVGELLLVNGELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYE 110
           R  E +  NG++YSG L G +P G GKY W DG IYEG+W  G   G GK+ + SGA YE
Sbjct: 4   RPAERVFSNGDVYSGQLKGTLPHGKGKYAWPDGIIYEGDWEEGKISGRGKLMWSSGAKYE 63

Query: 111 GEFSGGYMHGTGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKY 170
           G+FSGGY+HG GT   PD   Y G W++N++HGLG + Y N DV++GSW +G  +G G Y
Sbjct: 64  GDFSGGYLHGFGTLTSPDGSVYAGAWRMNVRHGLGRKEYCNSDVYDGSWREGLQDGSGSY 123

Query: 171 TWANGNVYLGNMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTS 230
           +W NGN ++GN K G MSG+G ++W NGD F G WL G+ HG GVY ++DGG Y G W+ 
Sbjct: 124 SWYNGNRFIGNWKKGKMSGRGVMSWANGDLFNGFWLNGLRHGSGVYKYADGGFYFGTWSR 183

Query: 231 GLKDGKGSFYPKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGEN 290
           GLKDG G FYP GS+ P+++                  K H H  +    D GNF +  N
Sbjct: 184 GLKDGSGVFYPAGSKHPSLK------------------KWHRHFGY---DDTGNFLLSHN 243

Query: 291 QQSSRVSSDKISKGNLLNLEQY-------RTKNVSLERRWSLEVSIEKVLGH-DSSSGLS 350
             +  +   + SK    +L +        RT     +  WS        + H  SS    
Sbjct: 244 -STINIDDLRTSKAVSRSLSELTTTSGLTRTSERYPDDYWSTSDPPRDFMHHGPSSKSAR 303

Query: 351 EYELGGNEIDTKIPIL-EREYMQGVLISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPG- 410
             + G +EI  K PI+ EREYMQGVLI E ++     S      + R   L KEV     
Sbjct: 304 SVDSGQSEIRDKNPIVFEREYMQGVLIKERIM-----SSIDMSHRARPLNLTKEVTVSAC 363

Query: 411 EMIIKGHRSYDLMLSLQLGIRYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPP 470
              + G  ++ LML+LQLGIRYTVGKITP+  REVR+SDF  RA   M FP+ GSQ TPP
Sbjct: 364 VSFLGGKWNHYLMLNLQLGIRYTVGKITPVPPREVRASDFSERARIMMFFPRNGSQYTPP 423

Query: 471 HQSEDFKWKDYCPMVFRNLREMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDR 530
           H+S DF WKDYCPMVFRNLREMFK+DAADYMMSICG+D LRE+SSPGKSGS+F+LS DDR
Sbjct: 424 HKSIDFDWKDYCPMVFRNLREMFKLDAADYMMSICGDDGLREISSPGKSGSIFYLSHDDR 483

Query: 531 FMIKTLRKSEVKVLLRMLPDYHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFC 590
           F+IKTL++SE+KVLLRMLP Y+ HV  YENTLITKFFG+HRIK   G+K RFVVMGNMFC
Sbjct: 484 FVIKTLKRSELKVLLRMLPRYYEHVGDYENTLITKFFGVHRIKLKWGKKVRFVVMGNMFC 543

Query: 591 TELRIHRRFDLKGSSLGRSADKVEIDENTILKDLDLNYSFYLEPTWRAALLKQIEIDSKF 650
           TEL+IHRR+DLKGS+ GR  +K +I E T LKDLDL Y F+++   R AL KQI +D  F
Sbjct: 544 TELKIHRRYDLKGSTQGRYTEKNKIGEKTTLKDLDLAYEFHMDKLLREALFKQIILDCSF 603

Query: 651 LEVQNIMDYSLLLGVHYRAPQQLQSHISYSRSM---RADGLGILAEDDPFEDDMSTYPQG 710
           LE   I+DYSLLLG+H+RAP  L   +     M    +D +G +  + P E   S  P+G
Sbjct: 604 LESLQILDYSLLLGLHFRAPDPLTDILEPPNEMSDQESDSVGSVDVNLPREP--SIPPKG 663

Query: 711 LVLVPRTDDNSV--VVGPHIRGSRLRAY--GDEEVDLLLPGTARLQIQLGVNMPARAEQI 770
           L++V   + NSV    GPHIRGS LRA+  G++EVDL+LPGTARL++QLGVNMPA+A   
Sbjct: 664 LLMVTH-EPNSVNTAPGPHIRGSTLRAFSVGEKEVDLILPGTARLRVQLGVNMPAQAHHK 723

Query: 771 PGKEEKQM----FHEAYDVVLYLGIIDILQEYNMSKKIEHAYKSIQFDSLSISAVDPTFY 827
            G++ ++       E YDVV+Y+GIIDILQEYNM KK+EH  KS+++D ++ISA++PT Y
Sbjct: 724 LGQDNEESGTVELFEVYDVVVYMGIIDILQEYNMKKKVEHTCKSMKYDPMTISAIEPTLY 769

BLAST of HG10020201 vs. TAIR 10
Match: AT1G60890.2 (Phosphatidylinositol-4-phosphate 5-kinase family protein )

HSP 1 Score: 769.2 bits (1985), Expect = 3.3e-222
Identity = 411/794 (51.76%), Postives = 524/794 (65.99%), Query Frame = 0

Query: 54  ELLLVNGELYSGSLLGNIPEGTGKYVWSDGCIYEGEWRRGMRQGTGKIRFPSGAVYEGEF 113
           E +  NG++YSG L G +P G GKY W DG IYEG+W  G   G GK+ + SGA YEG+F
Sbjct: 19  ERVFSNGDVYSGQLKGTLPHGKGKYAWPDGIIYEGDWEEGKISGRGKLMWSSGAKYEGDF 78

Query: 114 SGGYMHGTGTYIGPDNLTYKGRWKLNLKHGLGYQVYPNGDVFEGSWMQGTPEGPGKYTWA 173
           SGGY+HG GT   PD   Y G W++N++HGLG + Y N DV++GSW +G  +G G Y+W 
Sbjct: 79  SGGYLHGFGTLTSPDGSVYAGAWRMNVRHGLGRKEYCNSDVYDGSWREGLQDGSGSYSWY 138

Query: 174 NGNVYLGNMKAGGMSGKGTLTWINGDSFEGNWLGGMMHGFGVYMWSDGGCYVGMWTSGLK 233
           NGN ++GN K G MSG+G ++W NGD F G WL G+ HG GVY ++DGG Y G W+ GLK
Sbjct: 139 NGNRFIGNWKKGKMSGRGVMSWANGDLFNGFWLNGLRHGSGVYKYADGGFYFGTWSRGLK 198

Query: 234 DGKGSFYPKGSRLPAVQEIYLKALRKRGLLPDLKNKNHAHIHHATSGDMGNFKVGENQQS 293
           DG G FYP GS+ P+++                  K H H  +    D GNF +  N  +
Sbjct: 199 DGSGVFYPAGSKHPSLK------------------KWHRHFGY---DDTGNFLLSHN-ST 258

Query: 294 SRVSSDKISKGNLLNLEQY-------RTKNVSLERRWSLEVSIEKVLGH-DSSSGLSEYE 353
             +   + SK    +L +        RT     +  WS        + H  SS      +
Sbjct: 259 INIDDLRTSKAVSRSLSELTTTSGLTRTSERYPDDYWSTSDPPRDFMHHGPSSKSARSVD 318

Query: 354 LGGNEIDTKIPIL-EREYMQGVLISELVIDNSFTSPSSKRAKRRQRKLVKEVKKPG-EMI 413
            G +EI  K PI+ EREYMQGVLI E ++     S      + R   L KEV        
Sbjct: 319 SGQSEIRDKNPIVFEREYMQGVLIKERIM-----SSIDMSHRARPLNLTKEVTVSACVSF 378

Query: 414 IKGHRSYDLMLSLQLGIRYTVGKITPIQRREVRSSDFGPRASFWMNFPKEGSQLTPPHQS 473
           + G  ++ LML+LQLGIRYTVGKITP+  REVR+SDF  RA   M FP+ GSQ TPPH+S
Sbjct: 379 LGGKWNHYLMLNLQLGIRYTVGKITPVPPREVRASDFSERARIMMFFPRNGSQYTPPHKS 438

Query: 474 EDFKWKDYCPMVFRNLREMFKIDAADYMMSICGNDALRELSSPGKSGSVFFLSQDDRFMI 533
            DF WKDYCPMVFRNLREMFK+DAADYMMSICG+D LRE+SSPGKSGS+F+LS DDRF+I
Sbjct: 439 IDFDWKDYCPMVFRNLREMFKLDAADYMMSICGDDGLREISSPGKSGSIFYLSHDDRFVI 498

Query: 534 KTLRKSEVKVLLRMLPDYHHHVRSYENTLITKFFGLHRIKPSSGQKFRFVVMGNMFCTEL 593
           KTL++SE+KVLLRMLP Y+ HV  YENTLITKFFG+HRIK   G+K RFVVMGNMFCTEL
Sbjct: 499 KTLKRSELKVLLRMLPRYYEHVGDYENTLITKFFGVHRIKLKWGKKVRFVVMGNMFCTEL 558

Query: 594 RIHRRFDLKGSSLGRSADKVEIDENTILKDLDLNYSFYLEPTWRAALLKQIEIDSKFLEV 653
           +IHRR+DLKGS+ GR  +K +I E T LKDLDL Y F+++   R AL KQI +D  FLE 
Sbjct: 559 KIHRRYDLKGSTQGRYTEKNKIGEKTTLKDLDLAYEFHMDKLLREALFKQIILDCSFLES 618

Query: 654 QNIMDYSLLLGVHYRAPQQLQSHISYSRSM---RADGLGILAEDDPFEDDMSTYPQGLVL 713
             I+DYSLLLG+H+RAP  L   +     M    +D +G +  + P E   S  P+GL++
Sbjct: 619 LQILDYSLLLGLHFRAPDPLTDILEPPNEMSDQESDSVGSVDVNLPREP--SIPPKGLLM 678

Query: 714 VPRTDDNSV--VVGPHIRGSRLRAY--GDEEVDLLLPGTARLQIQLGVNMPARAEQIPGK 773
           V   + NSV    GPHIRGS LRA+  G++EVDL+LPGTARL++QLGVNMPA+A    G+
Sbjct: 679 VTH-EPNSVNTAPGPHIRGSTLRAFSVGEKEVDLILPGTARLRVQLGVNMPAQAHHKLGQ 738

Query: 774 EEKQM----FHEAYDVVLYLGIIDILQEYNMSKKIEHAYKSIQFDSLSISAVDPTFYSER 827
           + ++       E YDVV+Y+GIIDILQEYNM KK+EH  KS+++D ++ISA++PT YS+R
Sbjct: 739 DNEESGTVELFEVYDVVVYMGIIDILQEYNMKKKVEHTCKSMKYDPMTISAIEPTLYSKR 781