HG10012473 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10012473
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionProtein-serine/threonine kinase
LocationChr01: 21518878 .. 21521713 (-)
RNA-Seq ExpressionHG10012473
SyntenyHG10012473
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCTAAAAACCTCTGTGAAACCTTCTCCAAGAGCCTGATCGACGAGGTTCAGAAATGGGGTTGTATGAAACAGACTGGGGTTAGTTTGAGATACATGATGGAGTTTGGTTCCAAGCCCACTCCTAAGAATTTGCTTATTTCTGCTCAGTTTCTTCACAAGGAACTTCCAATTCGGATTGCTAGAAGAGCTATTGAACTTGAGAATCTTCCTTATGGCCTCTCTGTGAAACCTGCTGTTTTGAAGGTCTTTTTTCTTTACCTTACTCTTCTGTTTTTGTTTCCCCTTATATTTGCTTTGCATTTTGTTGAATTGGTTGTGGGTTCTTTCTGAATTGTCGAATTTATGAGGATTTTCTGACTGAGGAATTAGGAATCTGGTGATTGATGTAATGTTTTTTTTTTTGTTTCTGTCATAATTTCTCTTCACTTTTCTTCCGATTTTGGTTTCTTTTCATCATTTCTTGAGAATTTTCTGAAAATACTGTTTTGTTATGTTTATATTCTACTTGTTTGTTTGAAATTTCCAATTTGGGATGTGCATCTTTTTTACTGTACATGCTTGATTTAATTATACAGTGACTCAGTGAGAGCAGTTGGCTAAATTCTGCTCAATTATTTAATTCTCCATTCCTTGCTGTTCTAGAAGATTCCCTTTTGATTCAAACATCCTAAATTCTTTTGCTGGTTAACTGTTTCATGCAGAGCTGGACTTTTTTTTTTTTTTGAATTATTGTTTTTAAGAGTTCGAATTTTTGTTGTATTTTGTATAAAGTCAACATTTGGGGCATGTTTTCCTTGAAAAGTACTTGCATGAATAATGAAATGTTTAATTGTTTAAAGTACTTGCATCAAAATAATTTTCTGCTTTTTAGAATAAAAGTTCTGGGAAATTATTTTATGGTAATTGTTTTATAAAGTTGTTCTAAAACTCTTCTATGTTTCAGAAAAATGAACTTATTGTTCATCTCAAAATGACAAAATCACATTCTTGTGAATAGTGATTTCAATGTATACAATAATGTGAGAACTTTTACAAGTTCTTTAACCACAATCTTCGAAAACATTAAACTGTTTTTGAACCAGTTTTCCAATTAGTATTCCATTTGTTTCTTATTAGAGATTCACGGTGTACGAAAAGCAGAATTCTGTATATTATATTTTCTAGCACAACCTTTCCCTGGTTTTACTTTTCTCTAATGTTTTCTTAGGTTCGTGACTGGTATGTGGATTCTTTTCGTGACCTTCGGTCCTTTCCCGAGATAAAAAGTTCAGATGATGAAAAAGAGTTTACTCAAATGATAAAAGCAATTAAGGTGAGACACAACAACGTGGTTCAAACGATGGCTCTAGGTGTTCAACAGCTGAAGAAAGGCCTAGGTCTAAATAATATTGGATCTCTAGATCTTCACGAGATTCATCAGTTCTTGGATCGCTTCTACATGTCGAGAATTGGGATTCGCATGCTCATTGGTTGGTCAAATTCCTTAGACAAAAGTCCCAAGTCCCATATTTTCTGTCTTCTCCACTTCCTTCTCTATTTGTCTTTGTAATTTAAGATTTTCTTTGATGGGAGGAGGGGAAAAATCTAAATCATGCCATTATTTTGTGATTTCTAGAGGCTTAACATATGGTTTGTGAAATTTGATTGTCACTCAGGGCAGCATGTGGAATTACACAATCCCAATCCCCCTCCCGACCGTGTAGGATATATACACACAAAAATGTCCCCTGTTGATGTTGCGCAGAGCGCCAGTGAGGATGCTCGTGCAATCTGTTTACGTGAGTATGGTAGTGCCCCTAATATCAAAATCTATGGAGATCCGAGTTTTACATTTCCGTAAGTTCTTCATTTTGACTTTGCTCACGTGTTATTTACTATAAAGTTTCGCTCTTCTTGACTTCAATCTTTCTGTTCCGAGTGTTTCTGCTGATACTTATGCCATTTTTTGCCAGTGTGGTTACATATGGGCATGAATATTGACTTTAATCTCTCGTTTTTTGTTTACTTTTTCAGTTACGTTCCGACACACTTGCATCTTATGGTCTTTGAGTTGGTTAAAAACTCATTACGTGCCGTGCAAGAGTGTTTCATGGATTCTGATAAAGTTGCTCCTCCAGTTAGAATCATAGTTGCTGATGGAATTGAGGATGTCACTATTAAGGTATTGTTCTTGTTCATCACCAAAGTCAAGAGACTTCAATTTCTCAATGGAGTCTCAATATAAACTGTGTATATGACTGACTTGTAGGTATCAGATGAGGGCGGTGGCATACCCCGAAGCGGCCTTCCTAGAATTTTCACTTACCTCTACACCACTGCAAAAGACCCTCTGGACGAACATCCAGATCTGGGAACCGTGGATTTGGTGACAATGGCTGGATATGGTTATGGCCTTCCGATAAGCCGTCTGTATGCTCGTTATTTTGGTGGCGATCTGCAAGTGATCTCCATGGAAGGATATGGTAAGTAGAATCTAGCTGTTTTCAGTATTTTCAAGTACAGTACTGTGAATTCTGTTTCTTCGACACGGTTCACCTATTTCAATGCATCCAACCATTTCTCATCCAACTAGAGAATAAATAAAAGCTTCATGTTCCTTCATTGATTGGTTGTTTCGATGACGGTTACTCATTGTACTTAGTTTCAGTATCTATGGATGTAAATAATAGTTGAGTTGACATATCTGAGTGAGTTTTGAAATGTTCATTTCTAACCGGTCGGTATGGTCGTTTTCGGGGTGATAACTGCTGTTGTGATTGAACTGTACACAGGAACAGATGCATATCTTCACCTGTCTCGTCTGGGGGATTCACAAGAGCCTCTGCCATGA

mRNA sequence

ATGGCGGCTAAAAACCTCTGTGAAACCTTCTCCAAGAGCCTGATCGACGAGGTTCAGAAATGGGGTTGTATGAAACAGACTGGGGTTAGTTTGAGATACATGATGGAGTTTGGTTCCAAGCCCACTCCTAAGAATTTGCTTATTTCTGCTCAGTTTCTTCACAAGGAACTTCCAATTCGGATTGCTAGAAGAGCTATTGAACTTGAGAATCTTCCTTATGGCCTCTCTGTGAAACCTGCTGTTTTGAAGGTTCGTGACTGGTATGTGGATTCTTTTCGTGACCTTCGGTCCTTTCCCGAGATAAAAAGTTCAGATGATGAAAAAGAGTTTACTCAAATGATAAAAGCAATTAAGGTGAGACACAACAACGTGGTTCAAACGATGGCTCTAGGTGTTCAACAGCTGAAGAAAGGCCTAGGTCTAAATAATATTGGATCTCTAGATCTTCACGAGATTCATCAGTTCTTGGATCGCTTCTACATGTCGAGAATTGGGATTCGCATGCTCATTGGGCAGCATGTGGAATTACACAATCCCAATCCCCCTCCCGACCGTGTAGGATATATACACACAAAAATGTCCCCTGTTGATGTTGCGCAGAGCGCCAGTGAGGATGCTCGTGCAATCTGTTTACGTGAGTATGGTAGTGCCCCTAATATCAAAATCTATGGAGATCCGAGTTTTACATTTCCTTACGTTCCGACACACTTGCATCTTATGGTCTTTGAGTTGGTTAAAAACTCATTACGTGCCGTGCAAGAGTGTTTCATGGATTCTGATAAAGTTGCTCCTCCAGTTAGAATCATAGTTGCTGATGGAATTGAGGATGTCACTATTAAGGTATCAGATGAGGGCGGTGGCATACCCCGAAGCGGCCTTCCTAGAATTTTCACTTACCTCTACACCACTGCAAAAGACCCTCTGGACGAACATCCAGATCTGGGAACCGTGGATTTGGTGACAATGGCTGGATATGGTTATGGCCTTCCGATAAGCCGTCTGTATGCTCGTTATTTTGGTGGCGATCTGCAAGTGATCTCCATGGAAGGATATGGAACAGATGCATATCTTCACCTGTCTCGTCTGGGGGATTCACAAGAGCCTCTGCCATGA

Coding sequence (CDS)

ATGGCGGCTAAAAACCTCTGTGAAACCTTCTCCAAGAGCCTGATCGACGAGGTTCAGAAATGGGGTTGTATGAAACAGACTGGGGTTAGTTTGAGATACATGATGGAGTTTGGTTCCAAGCCCACTCCTAAGAATTTGCTTATTTCTGCTCAGTTTCTTCACAAGGAACTTCCAATTCGGATTGCTAGAAGAGCTATTGAACTTGAGAATCTTCCTTATGGCCTCTCTGTGAAACCTGCTGTTTTGAAGGTTCGTGACTGGTATGTGGATTCTTTTCGTGACCTTCGGTCCTTTCCCGAGATAAAAAGTTCAGATGATGAAAAAGAGTTTACTCAAATGATAAAAGCAATTAAGGTGAGACACAACAACGTGGTTCAAACGATGGCTCTAGGTGTTCAACAGCTGAAGAAAGGCCTAGGTCTAAATAATATTGGATCTCTAGATCTTCACGAGATTCATCAGTTCTTGGATCGCTTCTACATGTCGAGAATTGGGATTCGCATGCTCATTGGGCAGCATGTGGAATTACACAATCCCAATCCCCCTCCCGACCGTGTAGGATATATACACACAAAAATGTCCCCTGTTGATGTTGCGCAGAGCGCCAGTGAGGATGCTCGTGCAATCTGTTTACGTGAGTATGGTAGTGCCCCTAATATCAAAATCTATGGAGATCCGAGTTTTACATTTCCTTACGTTCCGACACACTTGCATCTTATGGTCTTTGAGTTGGTTAAAAACTCATTACGTGCCGTGCAAGAGTGTTTCATGGATTCTGATAAAGTTGCTCCTCCAGTTAGAATCATAGTTGCTGATGGAATTGAGGATGTCACTATTAAGGTATCAGATGAGGGCGGTGGCATACCCCGAAGCGGCCTTCCTAGAATTTTCACTTACCTCTACACCACTGCAAAAGACCCTCTGGACGAACATCCAGATCTGGGAACCGTGGATTTGGTGACAATGGCTGGATATGGTTATGGCCTTCCGATAAGCCGTCTGTATGCTCGTTATTTTGGTGGCGATCTGCAAGTGATCTCCATGGAAGGATATGGAACAGATGCATATCTTCACCTGTCTCGTCTGGGGGATTCACAAGAGCCTCTGCCATGA

Protein sequence

MAAKNLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIRIARRAIELENLPYGLSVKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVRHNNVVQTMALGVQQLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPNPPPDRVGYIHTKMSPVDVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLMVFELVKNSLRAVQECFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYLYTTAKDPLDEHPDLGTVDLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLSRLGDSQEPLP
Homology
BLAST of HG10012473 vs. NCBI nr
Match: XP_038894097.1 (pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial-like isoform X1 [Benincasa hispida])

HSP 1 Score: 741.5 bits (1913), Expect = 3.5e-210
Identity = 363/370 (98.11%), Postives = 366/370 (98.92%), Query Frame = 0

Query: 1   MAAKNLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60
           MAAK LCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSVKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120
           IARRAIELENLPYGLSVKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR
Sbjct: 61  IARRAIELENLPYGLSVKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120

Query: 121 HNNVVQTMALGVQQLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVV TMALGV+QLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPTMALGVRQLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDRVGYIHTKMSPVDVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240
           PPPDRVGYIHTKMSPV+VAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM
Sbjct: 181 PPPDRVGYIHTKMSPVEVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQECFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300
           VFELVKNSLRAVQE FMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL
Sbjct: 241 VFELVKNSLRAVQERFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300

Query: 301 YTTAKDPLDEHPDLGTVDLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTAKDPLDEHPDLGT DLVTMAGYGYGLPISRLYARYFGGDLQ+ISMEGYGTDAYLHLS
Sbjct: 301 YTTAKDPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQMISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 370

BLAST of HG10012473 vs. NCBI nr
Match: XP_004147627.2 (pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial [Cucumis sativus] >KGN57202.1 hypothetical protein Csa_011161 [Cucumis sativus])

HSP 1 Score: 730.3 bits (1884), Expect = 8.1e-207
Identity = 356/370 (96.22%), Postives = 364/370 (98.38%), Query Frame = 0

Query: 1   MAAKNLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60
           MAAK LCETFSK+LIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCETFSKTLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSVKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120
           IARRAIELENLPYGLS+KPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR
Sbjct: 61  IARRAIELENLPYGLSMKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120

Query: 121 HNNVVQTMALGVQQLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVV TMALGV+QLKKGLGLNN+GSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPTMALGVKQLKKGLGLNNVGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDRVGYIHTKMSPVDVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240
           PPPD VGYIHTKMSPV+VAQSASEDARAICLREYGSAP+IKIYGDPSFTFPYVPTHLHLM
Sbjct: 181 PPPDCVGYIHTKMSPVNVAQSASEDARAICLREYGSAPDIKIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQECFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300
           VFELVKNSLRAVQE FMDSDKV PPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL
Sbjct: 241 VFELVKNSLRAVQESFMDSDKVPPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300

Query: 301 YTTAKDPLDEHPDLGTVDLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTAK+PL+EHPDLGT DLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTAKEPLEEHPDLGTTDLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 370

BLAST of HG10012473 vs. NCBI nr
Match: XP_004147906.3 (pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial [Cucumis sativus] >KAE8649559.1 hypothetical protein Csa_018139 [Cucumis sativus])

HSP 1 Score: 717.6 bits (1851), Expect = 5.5e-203
Identity = 351/370 (94.86%), Postives = 360/370 (97.30%), Query Frame = 0

Query: 1   MAAKNLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60
           MAAK LCE+FSKSLID+VQKWGCMKQTGVSLRYMMEFGSKPT KNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCESFSKSLIDDVQKWGCMKQTGVSLRYMMEFGSKPTAKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSVKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120
           IARRAIELENLPYGLSVKPAVLKVRDWY+DSFRDLRSFPEIK+SDDEKEFTQMIKAIKVR
Sbjct: 61  IARRAIELENLPYGLSVKPAVLKVRDWYLDSFRDLRSFPEIKNSDDEKEFTQMIKAIKVR 120

Query: 121 HNNVVQTMALGVQQLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVV TMALGVQQLKKGL LNN+G  +LHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPTMALGVQQLKKGLRLNNVGYQNLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDRVGYIHTKMSPVDVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240
           PPPD VGYIHTKMSPV VA+SASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM
Sbjct: 181 PPPDCVGYIHTKMSPVKVAESASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQECFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300
           VFELVKNSLRAVQE FMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLP+IFTYL
Sbjct: 241 VFELVKNSLRAVQERFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300

Query: 301 YTTAKDPLDEHPDLGTVDLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTAK+PLDEHPDLGT DLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTAKNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 370

BLAST of HG10012473 vs. NCBI nr
Match: KAA0052157.1 (pyruvate dehydrogenase (acetyl-transferring) kinase [Cucumis melo var. makuwa] >TYK05122.1 pyruvate dehydrogenase (acetyl-transferring) kinase [Cucumis melo var. makuwa])

HSP 1 Score: 712.6 bits (1838), Expect = 1.8e-201
Identity = 347/370 (93.78%), Postives = 359/370 (97.03%), Query Frame = 0

Query: 1   MAAKNLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60
           MAAK LCE FSK+LIDEVQKWGCMKQTGVSLRYMMEFGS+PTPKNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCEAFSKTLIDEVQKWGCMKQTGVSLRYMMEFGSRPTPKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSVKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120
           IARR +ELENLPYGLS+KPAVLKVRDWYVDSFRD+RSFPEIKSSDDEKEFTQMIKA+KVR
Sbjct: 61  IARRVVELENLPYGLSLKPAVLKVRDWYVDSFRDVRSFPEIKSSDDEKEFTQMIKAVKVR 120

Query: 121 HNNVVQTMALGVQQLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVV TMALGV+QLKKGLGLNN+GS DLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPTMALGVKQLKKGLGLNNVGS-DLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDRVGYIHTKMSPVDVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240
           PPPD VGYIHTKMSPV+VAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM
Sbjct: 181 PPPDCVGYIHTKMSPVNVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQECFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300
           VFELVKNSLRAVQE F+DSDKV PPVRIIVADGIEDVTIKVSDEGGGIPRSGLP IFTYL
Sbjct: 241 VFELVKNSLRAVQEHFVDSDKVPPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPSIFTYL 300

Query: 301 YTTAKDPLDEHPDLGTVDLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTAK+PLDEHPDLGT + VTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTAKEPLDEHPDLGTTESVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 369

BLAST of HG10012473 vs. NCBI nr
Match: XP_008438992.1 (PREDICTED: pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 712.6 bits (1838), Expect = 1.8e-201
Identity = 347/370 (93.78%), Postives = 359/370 (97.03%), Query Frame = 0

Query: 1   MAAKNLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60
           MAAK LCE FSK+LIDEVQKWGCMKQTGVSLRYMMEFGS+PTPKNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCEAFSKTLIDEVQKWGCMKQTGVSLRYMMEFGSRPTPKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSVKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120
           IARR +ELENLPYGLS+KPAVLKVRDWYVDSFRD+RSFPEIKSSDDEKEFTQMIKA+KVR
Sbjct: 61  IARRVVELENLPYGLSLKPAVLKVRDWYVDSFRDVRSFPEIKSSDDEKEFTQMIKAVKVR 120

Query: 121 HNNVVQTMALGVQQLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVV TMALGV+QLKKGLGLNN+GS DLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPTMALGVKQLKKGLGLNNVGS-DLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDRVGYIHTKMSPVDVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240
           PPPD VGYIHTKMSPV+VAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM
Sbjct: 181 PPPDCVGYIHTKMSPVNVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQECFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300
           VFELVKNSLRAVQE F+DSDKV PPVRIIVADGIEDVTIKVSDEGGGIPRSGLP IFTYL
Sbjct: 241 VFELVKNSLRAVQERFVDSDKVPPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPSIFTYL 300

Query: 301 YTTAKDPLDEHPDLGTVDLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTAK+PLDEHPDLGT + VTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTAKEPLDEHPDLGTTESVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 369

BLAST of HG10012473 vs. ExPASy Swiss-Prot
Match: Q9SBJ1 ([Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PDK PE=1 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 1.5e-179
Identity = 308/371 (83.02%), Postives = 338/371 (91.11%), Query Frame = 0

Query: 1   MAAKNLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60
           MA K  CE F KSLI++V KWGCMKQTGVSLRYMMEFGSKPT +NLLISAQFLHKELPIR
Sbjct: 1   MAVKKACEMFPKSLIEDVHKWGCMKQTGVSLRYMMEFGSKPTERNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSVKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120
           +ARRAIEL+ LPYGLS KPAVLKVRDWY++SFRD+R+FPEIK S DEK+FTQMIKA+KVR
Sbjct: 61  VARRAIELQTLPYGLSDKPAVLKVRDWYLESFRDMRAFPEIKDSGDEKDFTQMIKAVKVR 120

Query: 121 HNNVVQTMALGVQQLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVV  MALGV QLKKG+   N G+LD  EIHQFLDRFY+SRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPMMALGVNQLKKGM---NSGNLD--EIHQFLDRFYLSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDRVGYIHTKMSPVDVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240
           PP   VGYIHTKMSP++VA++ASEDAR+IC REYGSAP I IYGDPSFTFPYVPTHLHLM
Sbjct: 181 PPLHTVGYIHTKMSPMEVARNASEDARSICFREYGSAPEINIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQECFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300
           ++ELVKNSLRAVQE F+DSD+VAPP+RIIVADGIEDVTIKVSDEGGGI RSGLPRIFTYL
Sbjct: 241 MYELVKNSLRAVQERFVDSDRVAPPIRIIVADGIEDVTIKVSDEGGGIARSGLPRIFTYL 300

Query: 301 YTTAKDPLDEHPDLGTVDL-VTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHL 360
           Y+TA++PL+E  DLG  D+ VTMAGYGYGLPISRLYARYFGGDLQ+ISMEGYGTDAYLHL
Sbjct: 301 YSTARNPLEEDVDLGIADVPVTMAGYGYGLPISRLYARYFGGDLQIISMEGYGTDAYLHL 360

Query: 361 SRLGDSQEPLP 371
           SRLGDSQEPLP
Sbjct: 361 SRLGDSQEPLP 366

BLAST of HG10012473 vs. ExPASy Swiss-Prot
Match: Q9P6P9 ([Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pkp1 PE=3 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 5.7e-70
Identity = 161/425 (37.88%), Postives = 231/425 (54.35%), Query Frame = 0

Query: 12  KSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIRIARRAIELENL 71
           K+L ++V       QTG+SL+ ++ FG  PTP  L  +  FL  ELPIR+ARR  +L+NL
Sbjct: 6   KTLQEKVNLLAQYPQTGLSLKQLVYFGKNPTPGTLFRAGLFLRDELPIRLARRIQDLQNL 65

Query: 72  PYGLSVKPAVLKVRDWYVDSFRDL------------------RSFPEIKSS--DDE---- 131
              L     +  V+  Y  S  ++                   + P+ +SS  D E    
Sbjct: 66  SPMLRSMKRISSVKAAYGRSMEEIIELKGVELPKCLPKHARYHNAPKWRSSLMDSEILHN 125

Query: 132 ---------------------------------------KEFTQMIKAIKVRHNNVVQTM 191
                                                    F  ++  I+ RH+NV   +
Sbjct: 126 PSLANTHLDSSKGRYFETDFSDQDNGVDCNWPESLLKFNSNFAYLLNTIRTRHDNVAVEI 185

Query: 192 ALGVQQLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPNPPPDRVGY 251
           AL +Q+ ++         +D + I  FLDRFYMSRIGIRML+GQ++ L +  P  + VG 
Sbjct: 186 ALDIQEYRR-----KTNQID-NSIQIFLDRFYMSRIGIRMLLGQYIALVSEPPRENYVGV 245

Query: 252 IHTKMSPVDVAQSASEDARAICLREYG--SAPNIKIYGDPSFTFPYVPTHLHLMVFELVK 311
           I T+ +   + + A+E+A+ IC   YG   AP I+I  DPS    YV +HL+  VFE++K
Sbjct: 246 ISTRANIYQIIEGAAENAKYICRLAYGLFEAPEIQIICDPSLEMMYVESHLNHAVFEILK 305

Query: 312 NSLRAVQECFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYLYTTAKD 370
           NSLRA  E         PP+++IVA G ED+TIK+SDEGGGI R  +P +++Y++TTA  
Sbjct: 306 NSLRATVEFHGVDSDFFPPIKVIVAKGQEDITIKISDEGGGISRRNIPLVWSYMFTTASP 365

BLAST of HG10012473 vs. ExPASy Swiss-Prot
Match: P91622 ([Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial OS=Drosophila melanogaster OX=7227 GN=Pdk PE=2 SV=2)

HSP 1 Score: 250.4 bits (638), Expect = 3.2e-65
Identity = 148/352 (42.05%), Postives = 208/352 (59.09%), Query Frame = 0

Query: 29  VSLRYMMEFGSKPTPKNLLISAQFLHKELPIRIARRAIELENLPYGLSVKPAVLKVRDWY 88
           +S++  M+FG     K   I   FL KELP+R+A    E+  LP  L    +V +V  WY
Sbjct: 33  LSIKQFMDFGQNACEKKSYI---FLRKELPVRLANIMKEIALLPDNLLHTRSVSEVSSWY 92

Query: 89  VDSFRDLRSFPEIKSSDDE-KEFTQMIKAIKVRHNNVVQTMALGVQQLKKGLGLNNIGSL 148
           V SF D+  + + + + D  ++F   +  I+ RHN+VVQTMA GV ++K+  G   + + 
Sbjct: 93  VKSFEDVLVYEKAEPTHDNLQKFVADLDLIRNRHNDVVQTMAQGVIEMKENEG-GQVDAP 152

Query: 149 DLHEIHQFLDRFYMSRIGIRMLIGQHVELH--NPNPPPDRVGYIHTKMSPVDVAQSASED 208
               I  FLDR YMSRI IRMLI QH  L   NP+     +G +       DV + A E+
Sbjct: 153 TESSIQYFLDRLYMSRISIRMLINQHTLLFGGNPHAGGRHIGCLDPACDLSDVVRDAYEN 212

Query: 209 ARAICLREYGSAPNIKIY---GDPSFTFP----YVPTHLHLMVFELVKNSLRAVQECF-M 268
           AR +C + Y ++P ++I     +P    P    YVP+HL+ M+FEL KNS+RAV E    
Sbjct: 213 ARFLCDQYYLTSPALEIQQHSSEPGDNLPIRTVYVPSHLYYMLFELFKNSMRAVVEHHGH 272

Query: 269 DSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYLYTTAKDPLDEHPDLGTV 328
           D++   PP+++ +  G ED+ +K+SD+GGGIPRS   ++F Y+Y+TA  P     DL TV
Sbjct: 273 DNNDTLPPLKVAICKGKEDICVKISDQGGGIPRSQTDQLFKYMYSTAPQP--SKSDLHTV 332

Query: 329 DLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLSRLGDSQEPL 370
            L   AGYGYGLPISRLYARYF GD+ ++S EG+GTDA ++L  L D    L
Sbjct: 333 PL---AGYGYGLPISRLYARYFHGDIVLLSCEGFGTDAIIYLKALSDEANEL 375

BLAST of HG10012473 vs. ExPASy Swiss-Prot
Match: Q63065 ([Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial OS=Rattus norvegicus OX=10116 GN=Pdk1 PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 3.6e-64
Identity = 148/356 (41.57%), Postives = 210/356 (58.99%), Query Frame = 0

Query: 29  VSLRYMMEFGSKPTPKNLLISAQFLHKELPIRIARRAIELENLPYGLSVKPAVLKVRDWY 88
           +S++  ++FGS    +    S  FL +ELP+R+A    E+  LP  L   P+V  V+ WY
Sbjct: 57  LSMKQFLDFGSVNACEK--TSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWY 116

Query: 89  VDSFRDLRSFPEIKSSDDEK---EFTQMIKAIKVRHNNVVQTMALGVQQLKKGLGLNNIG 148
           + S ++L  F + KS++D K   EFT  +  I+ RHN+V+ TMA GV + K+  G++ + 
Sbjct: 117 IQSLQELLDFKD-KSAEDAKTIYEFTDTVIRIRNRHNDVIPTMAQGVTEYKESFGVDPVT 176

Query: 149 SLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPNPPPD---RVGYIHTKMSPVDVAQSA 208
           S     +  FLDRFYMSRI IRML+ QH  L      P     +G I+     V+V +  
Sbjct: 177 S---QNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGSPSHRKHIGSINPNCDVVEVIKDG 236

Query: 209 SEDARAICLREYGSAP-------NIKIYGDPSFTFPYVPTHLHLMVFELVKNSLRAVQEC 268
            E+AR +C   Y ++P       N K  G P     YVP+HL+ MVFEL KN++RA  E 
Sbjct: 237 YENARRLCDLYYVNSPELELEELNAKSPGQP-IQVVYVPSHLYHMVFELFKNAMRATMEH 296

Query: 269 FMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYLYTTAKDPLDEHPDLG 328
             D   V PP+++ V  G ED+T+K+SD GGG+P   + R+F Y+Y+TA  P  E     
Sbjct: 297 HADKG-VYPPIQVHVTLGEEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVE----- 356

Query: 329 TVDLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLSRLG-DSQEPLP 371
           T   V +AG+GYGLPISRLYA+YF GDL++ S+EGYGTDA +++  L  +S E LP
Sbjct: 357 TSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTESIERLP 399

BLAST of HG10012473 vs. ExPASy Swiss-Prot
Match: Q8BFP9 ([Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial OS=Mus musculus OX=10090 GN=Pdk1 PE=1 SV=2)

HSP 1 Score: 246.5 bits (628), Expect = 4.7e-64
Identity = 146/355 (41.13%), Postives = 211/355 (59.44%), Query Frame = 0

Query: 29  VSLRYMMEFGSKPTPKNLLISAQFLHKELPIRIARRAIELENLPYGLSVKPAVLKVRDWY 88
           +S++  ++FGS    +    S  FL +ELP+R+A    E+  LP  L   P+V  V+ WY
Sbjct: 57  LSMKQFLDFGSVNACEK--TSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWY 116

Query: 89  VDSFRDLRSFPEIKSSDDEK---EFTQMIKAIKVRHNNVVQTMALGVQQLKKGLGLNNIG 148
           + S ++L  F + KS++D K   EFT  +  I+ RHN+V+ TMA GV + K+  G++ + 
Sbjct: 117 IQSLQELLDFKD-KSAEDAKTIYEFTDTVIRIRNRHNDVIPTMAQGVTEYKESFGVDPVT 176

Query: 149 SLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPNPPPD---RVGYIHTKMSPVDVAQSA 208
           S     +  FLDRFYMSRI IRML+ QH  L      P     +G I+     V+V +  
Sbjct: 177 S---QNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGSPSHRKHIGSINPNCDVVEVIKDG 236

Query: 209 SEDARAICLREYGSAPNIKI----YGDPSFTFP--YVPTHLHLMVFELVKNSLRAVQECF 268
            E+AR +C   Y ++P +++       P  T    YVP+HL+ MVFEL KN++RA  E  
Sbjct: 237 YENARRLCDLYYVNSPELELEELNAKSPGQTIQVVYVPSHLYHMVFELFKNAMRATMEHH 296

Query: 269 MDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYLYTTAKDPLDEHPDLGT 328
            D   V PP+++ V  G ED+T+K+SD GGG+P   + R+F Y+Y+TA  P  E     T
Sbjct: 297 ADKG-VYPPIQVHVTLGEEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVE-----T 356

Query: 329 VDLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLSRLG-DSQEPLP 371
              V +AG+GYGLPISRLYA+YF GDL++ S+EGYGTDA +++  L  +S E LP
Sbjct: 357 SRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTESVERLP 399

BLAST of HG10012473 vs. ExPASy TrEMBL
Match: A0A0A0L984 (Protein-serine/threonine kinase OS=Cucumis sativus OX=3659 GN=Csa_3G171100 PE=3 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 3.9e-207
Identity = 356/370 (96.22%), Postives = 364/370 (98.38%), Query Frame = 0

Query: 1   MAAKNLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60
           MAAK LCETFSK+LIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCETFSKTLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSVKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120
           IARRAIELENLPYGLS+KPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR
Sbjct: 61  IARRAIELENLPYGLSMKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120

Query: 121 HNNVVQTMALGVQQLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVV TMALGV+QLKKGLGLNN+GSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPTMALGVKQLKKGLGLNNVGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDRVGYIHTKMSPVDVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240
           PPPD VGYIHTKMSPV+VAQSASEDARAICLREYGSAP+IKIYGDPSFTFPYVPTHLHLM
Sbjct: 181 PPPDCVGYIHTKMSPVNVAQSASEDARAICLREYGSAPDIKIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQECFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300
           VFELVKNSLRAVQE FMDSDKV PPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL
Sbjct: 241 VFELVKNSLRAVQESFMDSDKVPPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300

Query: 301 YTTAKDPLDEHPDLGTVDLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTAK+PL+EHPDLGT DLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTAKEPLEEHPDLGTTDLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 370

BLAST of HG10012473 vs. ExPASy TrEMBL
Match: A0A0A0L2H2 (Protein-serine/threonine kinase OS=Cucumis sativus OX=3659 GN=Csa_4G304770 PE=3 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 1.3e-202
Identity = 350/370 (94.59%), Postives = 360/370 (97.30%), Query Frame = 0

Query: 1   MAAKNLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60
           MAAK LCE+FSKSLID+VQKWGCMKQTGVSLRYMMEFGSKPT KNLLISAQFL+KELPIR
Sbjct: 1   MAAKKLCESFSKSLIDDVQKWGCMKQTGVSLRYMMEFGSKPTAKNLLISAQFLYKELPIR 60

Query: 61  IARRAIELENLPYGLSVKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120
           IARRAIELENLPYGLSVKPAVLKVRDWY+DSFRDLRSFPEIK+SDDEKEFTQMIKAIKVR
Sbjct: 61  IARRAIELENLPYGLSVKPAVLKVRDWYLDSFRDLRSFPEIKNSDDEKEFTQMIKAIKVR 120

Query: 121 HNNVVQTMALGVQQLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVV TMALGVQQLKKGL LNN+G  +LHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPTMALGVQQLKKGLRLNNVGYQNLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDRVGYIHTKMSPVDVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240
           PPPD VGYIHTKMSPV VA+SASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM
Sbjct: 181 PPPDCVGYIHTKMSPVKVAESASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQECFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300
           VFELVKNSLRAVQE FMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLP+IFTYL
Sbjct: 241 VFELVKNSLRAVQERFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPKIFTYL 300

Query: 301 YTTAKDPLDEHPDLGTVDLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTAK+PLDEHPDLGT DLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTAKNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 370

BLAST of HG10012473 vs. ExPASy TrEMBL
Match: A0A5A7UCH7 (Protein-serine/threonine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1769G00260 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 8.5e-202
Identity = 347/370 (93.78%), Postives = 359/370 (97.03%), Query Frame = 0

Query: 1   MAAKNLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60
           MAAK LCE FSK+LIDEVQKWGCMKQTGVSLRYMMEFGS+PTPKNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCEAFSKTLIDEVQKWGCMKQTGVSLRYMMEFGSRPTPKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSVKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120
           IARR +ELENLPYGLS+KPAVLKVRDWYVDSFRD+RSFPEIKSSDDEKEFTQMIKA+KVR
Sbjct: 61  IARRVVELENLPYGLSLKPAVLKVRDWYVDSFRDVRSFPEIKSSDDEKEFTQMIKAVKVR 120

Query: 121 HNNVVQTMALGVQQLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVV TMALGV+QLKKGLGLNN+GS DLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPTMALGVKQLKKGLGLNNVGS-DLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDRVGYIHTKMSPVDVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240
           PPPD VGYIHTKMSPV+VAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM
Sbjct: 181 PPPDCVGYIHTKMSPVNVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQECFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300
           VFELVKNSLRAVQE F+DSDKV PPVRIIVADGIEDVTIKVSDEGGGIPRSGLP IFTYL
Sbjct: 241 VFELVKNSLRAVQEHFVDSDKVPPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPSIFTYL 300

Query: 301 YTTAKDPLDEHPDLGTVDLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTAK+PLDEHPDLGT + VTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTAKEPLDEHPDLGTTESVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 369

BLAST of HG10012473 vs. ExPASy TrEMBL
Match: A0A1S3AYB5 (Protein-serine/threonine kinase OS=Cucumis melo OX=3656 GN=LOC103483922 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 8.5e-202
Identity = 347/370 (93.78%), Postives = 359/370 (97.03%), Query Frame = 0

Query: 1   MAAKNLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60
           MAAK LCE FSK+LIDEVQKWGCMKQTGVSLRYMMEFGS+PTPKNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCEAFSKTLIDEVQKWGCMKQTGVSLRYMMEFGSRPTPKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSVKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120
           IARR +ELENLPYGLS+KPAVLKVRDWYVDSFRD+RSFPEIKSSDDEKEFTQMIKA+KVR
Sbjct: 61  IARRVVELENLPYGLSLKPAVLKVRDWYVDSFRDVRSFPEIKSSDDEKEFTQMIKAVKVR 120

Query: 121 HNNVVQTMALGVQQLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVV TMALGV+QLKKGLGLNN+GS DLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPTMALGVKQLKKGLGLNNVGS-DLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDRVGYIHTKMSPVDVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240
           PPPD VGYIHTKMSPV+VAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM
Sbjct: 181 PPPDCVGYIHTKMSPVNVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQECFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300
           VFELVKNSLRAVQE F+DSDKV PPVRIIVADGIEDVTIKVSDEGGGIPRSGLP IFTYL
Sbjct: 241 VFELVKNSLRAVQERFVDSDKVPPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPSIFTYL 300

Query: 301 YTTAKDPLDEHPDLGTVDLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTAK+PLDEHPDLGT + VTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTAKEPLDEHPDLGTTESVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 369

BLAST of HG10012473 vs. ExPASy TrEMBL
Match: A0A6J1IPR6 (Protein-serine/threonine kinase OS=Cucurbita maxima OX=3661 GN=LOC111479408 PE=3 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 3.2e-201
Identity = 348/370 (94.05%), Postives = 356/370 (96.22%), Query Frame = 0

Query: 1   MAAKNLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60
           MAAK LCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFG KPTPKNLLISAQFLHKELPIR
Sbjct: 1   MAAKKLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGLKPTPKNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSVKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120
           IARRAIELENLPYGLSVKPAVLKVRDWY+DSFRDLR+FPEIK+ DDEKEFTQMIKAIKVR
Sbjct: 61  IARRAIELENLPYGLSVKPAVLKVRDWYLDSFRDLRAFPEIKNPDDEKEFTQMIKAIKVR 120

Query: 121 HNNVVQTMALGVQQLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVV  MALGVQQLKK + L+NIG  DLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPMMALGVQQLKKSIALDNIGYQDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDRVGYIHTKMSPVDVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240
           PPPD VGYIHTKMSPV VA+SASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHL LM
Sbjct: 181 PPPDCVGYIHTKMSPVKVAESASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLQLM 240

Query: 241 VFELVKNSLRAVQECFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300
           VFELVKNSLRAVQE FMDSDKVAPPV+IIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL
Sbjct: 241 VFELVKNSLRAVQERFMDSDKVAPPVKIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300

Query: 301 YTTAKDPLDEHPDLGTVDLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360
           YTTAK+PLDEHPDLGT DLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS
Sbjct: 301 YTTAKNPLDEHPDLGTADLVTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHLS 360

Query: 361 RLGDSQEPLP 371
           RLGDSQEPLP
Sbjct: 361 RLGDSQEPLP 370

BLAST of HG10012473 vs. TAIR 10
Match: AT3G06483.1 (pyruvate dehydrogenase kinase )

HSP 1 Score: 630.2 bits (1624), Expect = 1.1e-180
Identity = 308/371 (83.02%), Postives = 338/371 (91.11%), Query Frame = 0

Query: 1   MAAKNLCETFSKSLIDEVQKWGCMKQTGVSLRYMMEFGSKPTPKNLLISAQFLHKELPIR 60
           MA K  CE F KSLI++V KWGCMKQTGVSLRYMMEFGSKPT +NLLISAQFLHKELPIR
Sbjct: 1   MAVKKACEMFPKSLIEDVHKWGCMKQTGVSLRYMMEFGSKPTERNLLISAQFLHKELPIR 60

Query: 61  IARRAIELENLPYGLSVKPAVLKVRDWYVDSFRDLRSFPEIKSSDDEKEFTQMIKAIKVR 120
           +ARRAIEL+ LPYGLS KPAVLKVRDWY++SFRD+R+FPEIK S DEK+FTQMIKA+KVR
Sbjct: 61  VARRAIELQTLPYGLSDKPAVLKVRDWYLESFRDMRAFPEIKDSGDEKDFTQMIKAVKVR 120

Query: 121 HNNVVQTMALGVQQLKKGLGLNNIGSLDLHEIHQFLDRFYMSRIGIRMLIGQHVELHNPN 180
           HNNVV  MALGV QLKKG+   N G+LD  EIHQFLDRFY+SRIGIRMLIGQHVELHNPN
Sbjct: 121 HNNVVPMMALGVNQLKKGM---NSGNLD--EIHQFLDRFYLSRIGIRMLIGQHVELHNPN 180

Query: 181 PPPDRVGYIHTKMSPVDVAQSASEDARAICLREYGSAPNIKIYGDPSFTFPYVPTHLHLM 240
           PP   VGYIHTKMSP++VA++ASEDAR+IC REYGSAP I IYGDPSFTFPYVPTHLHLM
Sbjct: 181 PPLHTVGYIHTKMSPMEVARNASEDARSICFREYGSAPEINIYGDPSFTFPYVPTHLHLM 240

Query: 241 VFELVKNSLRAVQECFMDSDKVAPPVRIIVADGIEDVTIKVSDEGGGIPRSGLPRIFTYL 300
           ++ELVKNSLRAVQE F+DSD+VAPP+RIIVADGIEDVTIKVSDEGGGI RSGLPRIFTYL
Sbjct: 241 MYELVKNSLRAVQERFVDSDRVAPPIRIIVADGIEDVTIKVSDEGGGIARSGLPRIFTYL 300

Query: 301 YTTAKDPLDEHPDLGTVDL-VTMAGYGYGLPISRLYARYFGGDLQVISMEGYGTDAYLHL 360
           Y+TA++PL+E  DLG  D+ VTMAGYGYGLPISRLYARYFGGDLQ+ISMEGYGTDAYLHL
Sbjct: 301 YSTARNPLEEDVDLGIADVPVTMAGYGYGLPISRLYARYFGGDLQIISMEGYGTDAYLHL 360

Query: 361 SRLGDSQEPLP 371
           SRLGDSQEPLP
Sbjct: 361 SRLGDSQEPLP 366

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894097.13.5e-21098.11pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial-like isoform ... [more]
XP_004147627.28.1e-20796.22pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial [Cucumis sati... [more]
XP_004147906.35.5e-20394.86pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial [Cucumis sati... [more]
KAA0052157.11.8e-20193.78pyruvate dehydrogenase (acetyl-transferring) kinase [Cucumis melo var. makuwa] >... [more]
XP_008438992.11.8e-20193.78PREDICTED: pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial is... [more]
Match NameE-valueIdentityDescription
Q9SBJ11.5e-17983.02[Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial OS=Arabidop... [more]
Q9P6P95.7e-7037.88[Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial OS=Schizosa... [more]
P916223.2e-6542.05[Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial OS=Drosophi... [more]
Q630653.6e-6441.57[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial O... [more]
Q8BFP94.7e-6441.13[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial O... [more]
Match NameE-valueIdentityDescription
A0A0A0L9843.9e-20796.22Protein-serine/threonine kinase OS=Cucumis sativus OX=3659 GN=Csa_3G171100 PE=3 ... [more]
A0A0A0L2H21.3e-20294.59Protein-serine/threonine kinase OS=Cucumis sativus OX=3659 GN=Csa_4G304770 PE=3 ... [more]
A0A5A7UCH78.5e-20293.78Protein-serine/threonine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3AYB58.5e-20293.78Protein-serine/threonine kinase OS=Cucumis melo OX=3656 GN=LOC103483922 PE=3 SV=... [more]
A0A6J1IPR63.2e-20194.05Protein-serine/threonine kinase OS=Cucurbita maxima OX=3661 GN=LOC111479408 PE=3... [more]
Match NameE-valueIdentityDescription
AT3G06483.11.1e-18083.02pyruvate dehydrogenase kinase [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004358Signal transduction histidine kinase-related protein, C-terminalPRINTSPR00344BCTRLSENSORcoord: 347..360
score: 28.11
coord: 277..291
score: 40.34
coord: 323..341
score: 35.59
IPR003594Histidine kinase/HSP90-like ATPaseSMARTSM00387HKATPase_4coord: 232..363
e-value: 6.7E-11
score: 52.2
IPR003594Histidine kinase/HSP90-like ATPasePFAMPF02518HATPase_ccoord: 234..360
e-value: 1.1E-14
score: 54.9
IPR036890Histidine kinase/HSP90-like ATPase superfamilyGENE3D3.30.565.10coord: 192..369
e-value: 1.5E-51
score: 176.2
IPR036890Histidine kinase/HSP90-like ATPase superfamilySUPERFAMILY55874ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinasecoord: 188..359
IPR036784Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain superfamilyGENE3D1.20.140.20coord: 4..183
e-value: 9.8E-63
score: 213.2
IPR036784Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain superfamilySUPERFAMILY69012alpha-ketoacid dehydrogenase kinase, N-terminal domaincoord: 16..180
IPR018955Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase, N-terminalPFAMPF10436BCDHK_Adom3coord: 28..189
e-value: 3.1E-55
score: 186.5
IPR039028PDK/BCKDK protein kinasePANTHERPTHR11947PYRUVATE DEHYDROGENASE KINASEcoord: 10..370
NoneNo IPR availablePANTHERPTHR11947:SF42BNAA05G30880D PROTEINcoord: 10..370
NoneNo IPR availableCDDcd16929HATPase_PDK-likecoord: 194..360
e-value: 1.02651E-77
score: 234.927
IPR005467Histidine kinase domainPROSITEPS50109HIS_KINcoord: 234..363
score: 17.719801

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10012473.1HG10012473.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0010906 regulation of glucose metabolic process
biological_process GO:0016310 phosphorylation
cellular_component GO:0005759 mitochondrial matrix
molecular_function GO:0005524 ATP binding
molecular_function GO:0009927 histidine phosphotransfer kinase activity
molecular_function GO:0042803 protein homodimerization activity
molecular_function GO:0004740 pyruvate dehydrogenase (acetyl-transferring) kinase activity
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0016772 transferase activity, transferring phosphorus-containing groups