Moc10g05770 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc10g05770 vs. NCBI nr
Match: XP_022136725.1 (mechanosensitive ion channel protein 2, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1174.5 bits (3037), Expect = 0.0e+00
Identity = 608/608 (100.00%), Postives = 608/608 (100.00%), Query Frame = 0

Query: 1   MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 60
           MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL
Sbjct: 124 MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 183

Query: 61  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 120
           PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA
Sbjct: 184 PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 243

Query: 121 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 180
           VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV
Sbjct: 244 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 303

Query: 181 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 240
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK
Sbjct: 304 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 363

Query: 241 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 300
           THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK
Sbjct: 364 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 423

Query: 301 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 360
           TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV
Sbjct: 424 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 483

Query: 361 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 420
           NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE
Sbjct: 484 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 543

Query: 421 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 480
           VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT
Sbjct: 544 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 603

Query: 481 DNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKP 540
           DNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKP
Sbjct: 604 DNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKP 663

Query: 541 STLKPGVEENLILGVALDGSKRTLPIEEDMPSSPETVKDLAARWNGNGTNGATTPDKDTK 600
           STLKPGVEENLILGVALDGSKRTLPIEEDMPSSPETVKDLAARWNGNGTNGATTPDKDTK
Sbjct: 664 STLKPGVEENLILGVALDGSKRTLPIEEDMPSSPETVKDLAARWNGNGTNGATTPDKDTK 723

Query: 601 DQTPPPSE 609
           DQTPPPSE
Sbjct: 724 DQTPPPSE 731

BLAST of Moc10g05770 vs. NCBI nr
Match: KAG6577342.1 (Mechanosensitive ion channel protein 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 979.5 bits (2531), Expect = 1.3e-281
Identity = 522/612 (85.29%), Postives = 558/612 (91.18%), Query Frame = 0

Query: 1   MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 60
           +FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW G  LICRALDPVVL
Sbjct: 124 IFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVVL 183

Query: 61  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 120
           PT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+ESSDARNMGFQFAWKA
Sbjct: 184 PTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWKA 243

Query: 121 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 180
           VYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPF+V
Sbjct: 244 VYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 303

Query: 181 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 240
           NEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIK
Sbjct: 304 NEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIK 363

Query: 241 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 300
           THLAISHLDV+KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK
Sbjct: 364 THLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 423

Query: 301 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 360
           TSHFEEYLCVKEAI LDLLRVIRHHRARLATPIRT+QKMHSDSDLESVPFSDSIFG GG 
Sbjct: 424 TSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGA 483

Query: 361 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 420
            LNRRMLMIEPPYKVYG+DRKQSHSRTSRTTGEQNGKPIARSSGD+KAAKE TPS+RKTE
Sbjct: 484 TLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTE 543

Query: 421 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 480
           V+TGE +DSD K H KVP S S+D  S+ESKHKSSSRS SSTNGI+ MP+SDAKTT SD 
Sbjct: 544 VKTGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDA 603

Query: 481 DNSFEDP-STKQSENGSGS-TKQNYKSNHPAVSLPEDVKK-TGGSSATSQPRIEGEQTPV 540
           DNS +D  S+K+S+N SGS  KQNYK  H +VS  EDVKK  G +S+ SQPRIEG+QT  
Sbjct: 604 DNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT-- 663

Query: 541 LKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPET---VKDL-AARWNGNGTNGAT 600
             PST KPGVEEN+ILGVALDGSKRTLPIE+D PSSP+T    KDL AA  NGNG NGAT
Sbjct: 664 TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGANGAT 723

Query: 601 TPDKDTKDQTPP 606
           TPDK+TK Q+PP
Sbjct: 724 TPDKETKRQSPP 731

BLAST of Moc10g05770 vs. NCBI nr
Match: XP_022929456.1 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 972.2 bits (2512), Expect = 2.0e-279
Identity = 519/612 (84.80%), Postives = 555/612 (90.69%), Query Frame = 0

Query: 1   MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 60
           +FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW G  LICRALDPVVL
Sbjct: 104 IFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVVL 163

Query: 61  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 120
           PT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+ESSDARNMGFQFAWKA
Sbjct: 164 PTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWKA 223

Query: 121 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 180
           VYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPF+V
Sbjct: 224 VYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 283

Query: 181 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 240
           NEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIK
Sbjct: 284 NEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIK 343

Query: 241 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 300
           THLAISHLDV+KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK
Sbjct: 344 THLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 403

Query: 301 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 360
           TSHFEEYLCVKEAI LDLLRVIRHHRARLATPIRT+QKMHSDSDLESVPFSDSIFG GG 
Sbjct: 404 TSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGA 463

Query: 361 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 420
            LNRRMLMIEPPYKVYG+DRKQSHSRTSRTTGEQNGKPIARSSGD+KAAKE TPS+RKTE
Sbjct: 464 TLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTE 523

Query: 421 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 480
           V+ GE +DSD K H KVP S S+D  S+ESKHKSSSRS SSTNGI+ MP+SDAKTT SD 
Sbjct: 524 VKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDA 583

Query: 481 DNSFEDP-STKQSENGSGS-TKQNYKSNHPAVSLPEDVKK-TGGSSATSQPRIEGEQTPV 540
           DNS +D  S+K+S+N SGS  KQNYK  H +VS  EDVKK  G +S+ SQPRIEG+QT  
Sbjct: 584 DNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT-- 643

Query: 541 LKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPET---VKDL-AARWNGNGTNGAT 600
             PST KPGVEEN+ILGVALDGSKRTLPIE+D PSSP+T    KDL AA  NGNG  GAT
Sbjct: 644 TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGAT 703

Query: 601 TPDKDTKDQTPP 606
           TPDK+TK  +PP
Sbjct: 704 TPDKETKRLSPP 711

BLAST of Moc10g05770 vs. NCBI nr
Match: XP_022929450.1 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929451.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929452.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929453.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929454.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929455.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 972.2 bits (2512), Expect = 2.0e-279
Identity = 519/612 (84.80%), Postives = 555/612 (90.69%), Query Frame = 0

Query: 1   MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 60
           +FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW G  LICRALDPVVL
Sbjct: 124 IFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVVL 183

Query: 61  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 120
           PT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+ESSDARNMGFQFAWKA
Sbjct: 184 PTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWKA 243

Query: 121 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 180
           VYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPF+V
Sbjct: 244 VYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 303

Query: 181 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 240
           NEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIK
Sbjct: 304 NEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIK 363

Query: 241 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 300
           THLAISHLDV+KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK
Sbjct: 364 THLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 423

Query: 301 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 360
           TSHFEEYLCVKEAI LDLLRVIRHHRARLATPIRT+QKMHSDSDLESVPFSDSIFG GG 
Sbjct: 424 TSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGA 483

Query: 361 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 420
            LNRRMLMIEPPYKVYG+DRKQSHSRTSRTTGEQNGKPIARSSGD+KAAKE TPS+RKTE
Sbjct: 484 TLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTE 543

Query: 421 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 480
           V+ GE +DSD K H KVP S S+D  S+ESKHKSSSRS SSTNGI+ MP+SDAKTT SD 
Sbjct: 544 VKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDA 603

Query: 481 DNSFEDP-STKQSENGSGS-TKQNYKSNHPAVSLPEDVKK-TGGSSATSQPRIEGEQTPV 540
           DNS +D  S+K+S+N SGS  KQNYK  H +VS  EDVKK  G +S+ SQPRIEG+QT  
Sbjct: 604 DNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT-- 663

Query: 541 LKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPET---VKDL-AARWNGNGTNGAT 600
             PST KPGVEEN+ILGVALDGSKRTLPIE+D PSSP+T    KDL AA  NGNG  GAT
Sbjct: 664 TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGAT 723

Query: 601 TPDKDTKDQTPP 606
           TPDK+TK  +PP
Sbjct: 724 TPDKETKRLSPP 731

BLAST of Moc10g05770 vs. NCBI nr
Match: XP_022929458.1 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 972.2 bits (2512), Expect = 2.0e-279
Identity = 519/612 (84.80%), Postives = 555/612 (90.69%), Query Frame = 0

Query: 1   MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 60
           +FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW G  LICRALDPVVL
Sbjct: 9   IFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVVL 68

Query: 61  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 120
           PT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+ESSDARNMGFQFAWKA
Sbjct: 69  PTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWKA 128

Query: 121 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 180
           VYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPF+V
Sbjct: 129 VYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 188

Query: 181 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 240
           NEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIK
Sbjct: 189 NEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIK 248

Query: 241 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 300
           THLAISHLDV+KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK
Sbjct: 249 THLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 308

Query: 301 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 360
           TSHFEEYLCVKEAI LDLLRVIRHHRARLATPIRT+QKMHSDSDLESVPFSDSIFG GG 
Sbjct: 309 TSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGA 368

Query: 361 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 420
            LNRRMLMIEPPYKVYG+DRKQSHSRTSRTTGEQNGKPIARSSGD+KAAKE TPS+RKTE
Sbjct: 369 TLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTE 428

Query: 421 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 480
           V+ GE +DSD K H KVP S S+D  S+ESKHKSSSRS SSTNGI+ MP+SDAKTT SD 
Sbjct: 429 VKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDA 488

Query: 481 DNSFEDP-STKQSENGSGS-TKQNYKSNHPAVSLPEDVKK-TGGSSATSQPRIEGEQTPV 540
           DNS +D  S+K+S+N SGS  KQNYK  H +VS  EDVKK  G +S+ SQPRIEG+QT  
Sbjct: 489 DNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT-- 548

Query: 541 LKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPET---VKDL-AARWNGNGTNGAT 600
             PST KPGVEEN+ILGVALDGSKRTLPIE+D PSSP+T    KDL AA  NGNG  GAT
Sbjct: 549 TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGAT 608

Query: 601 TPDKDTKDQTPP 606
           TPDK+TK  +PP
Sbjct: 609 TPDKETKRLSPP 616

BLAST of Moc10g05770 vs. ExPASy Swiss-Prot
Match: Q56X46 (Mechanosensitive ion channel protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MSL2 PE=2 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 5.5e-187
Identity = 364/608 (59.87%), Postives = 431/608 (70.89%), Query Frame = 0

Query: 1   MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 60
           +F++WGL PF R  R++LL+ +DN WKKS TY VMT Y+QPLLLW G   ICRALDPVVL
Sbjct: 121 VFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVL 180

Query: 61  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 120
           PT+ S++VK R+LNFVRSLSTVLAFAYCLSS+I+Q QK FSET+  SD RNMGFQFA KA
Sbjct: 181 PTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKA 240

Query: 121 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 180
           +YSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRPF++
Sbjct: 241 LYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVL 300

Query: 181 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 240
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREA+HIPNH+FT+NVVRNL+QKTHWRIK
Sbjct: 301 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIK 360

Query: 241 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 300
           THLAISHLDVNKINNIVADMRKVLAKNP VEQQRLHRRVFLENV PENQAL ILISCFVK
Sbjct: 361 THLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVK 420

Query: 301 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 360
           TSH EEYL VKEAI+LDLLRVI HHRARLATPIRT++KM++++D+E+ PF +S++  GGV
Sbjct: 421 TSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGESMY--GGV 480

Query: 361 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 420
              R +++IEP YK+ GED+ +S +R ++ T EQ  K      G +  +KE +  D K  
Sbjct: 481 TSRRPLMLIEPAYKINGEDKSKSQNRAAKPTAEQENK------GSNPKSKETSSPDLKAN 540

Query: 421 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 480
           V+ GE   SDT K P+                                     +T     
Sbjct: 541 VKVGESPVSDTNKVPE-------------------------------------ETVAKPV 600

Query: 481 DNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKP 540
             +   P T +    SG+ K                K++GG+  +++       T     
Sbjct: 601 IKAVSKPPTPKDTETSGTEKPK-------------AKRSGGTIKSTKTDETDSSTSSASR 660

Query: 541 STLKPGVEENLILGVALDGSKRTLPIEEDMPSSP-ETVKDLAARWNGNGTNGATTPDKDT 600
           STL    EEN++LGVAL+GSKRTLPIEE++ S P ET          +G NG    DK+ 
Sbjct: 661 STL----EENIVLGVALEGSKRTLPIEEEIHSPPMETDAKELTGARRSGGNGPLVADKEQ 666

Query: 601 KDQTPPPS 608
           KD    P+
Sbjct: 721 KDSQSQPN 666

BLAST of Moc10g05770 vs. ExPASy Swiss-Prot
Match: Q8L7W1 (Mechanosensitive ion channel protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MSL3 PE=1 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 9.4e-163
Identity = 327/580 (56.38%), Postives = 406/580 (70.00%), Query Frame = 0

Query: 2   FAVWGLGPFLRYTRSLLLH-GSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 61
           FA WGL P LR  R+ L   G+D N +KS T  ++  YLQPLLLW+G  L+CR LDP+VL
Sbjct: 123 FATWGLRPLLRLARTTLFEKGNDANSQKSSTQYIVVSYLQPLLLWSGAILLCRTLDPIVL 182

Query: 62  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 121
           P+   Q +KQR+L F RS+STVLAF+ CLSS+++Q QKFF ET   +D RNMGF FA KA
Sbjct: 183 PSSAGQAIKQRLLIFARSISTVLAFSCCLSSLLQQVQKFFMETNNPADTRNMGFSFAGKA 242

Query: 122 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 181
           VY+A WVAA SLFMELLGFSTQKWLTAGGLGTVLLTLAGREI TNFLSS+MIHATRPF++
Sbjct: 243 VYTAAWVAAASLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATRPFVL 302

Query: 182 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 241
           NEWIQTKI GYEVSGTVE VGWWSPTIIRG+DREAVHIPNHQF++N+VRNL+QKTHWRIK
Sbjct: 303 NEWIQTKIGGYEVSGTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWRIK 362

Query: 242 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 301
           THLAISHLDV+KINNIVADMRKVL+KNPQ+EQQ++HRRVFLE+++PENQAL ILISCFVK
Sbjct: 363 THLAISHLDVSKINNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALRILISCFVK 422

Query: 302 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 361
           TS FEEYLCVKEA++LDLL VIRHH ARLATPIRT+Q+M +++++++  FSD +F +   
Sbjct: 423 TSRFEEYLCVKEAVLLDLLTVIRHHGARLATPIRTVQRMRNEAEVDTAGFSDIVFNQAA- 482

Query: 362 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 421
            +NRR ++IEP YK+  +D  +S    S + G+++  P  +S    +   +  PS+ K E
Sbjct: 483 -MNRRYMLIEPSYKINSDDNSKS---PSPSPGQKSPSPGQKS---EERDLQEEPSETKAE 542

Query: 422 V-RTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSS------RSASSTNGISGMPSSDA 481
               G    S+ KK  +     S+ N+  +    S+S      +S        G P    
Sbjct: 543 TENNGSVPVSNAKKENQKAALGSNSNTGTKGSSTSTSDQPVAQKSEEKKKESVGDPHKAE 602

Query: 482 KTTRSDTDNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGE 541
           K   SD + + E   T +S+   GS K N +S            + GG S TS       
Sbjct: 603 KDEVSDDEATIE--QTLKSKAKQGSEKNNGESK----------ARDGGGSGTSSL----- 662

Query: 542 QTPVLKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSS 574
                        +EENL+LGVALDGSKRTLPI+E+  +S
Sbjct: 663 -------------LEENLVLGVALDGSKRTLPIDEEHKAS 664

BLAST of Moc10g05770 vs. ExPASy Swiss-Prot
Match: P0AEB6 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=83334 GN=ynaI PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 4.3e-22
Identity = 66/211 (31.28%), Postives = 116/211 (54.98%), Query Frame = 0

Query: 127 VAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIVNEWIQT 186
           V  V L+ E  G S    LT GG+G + + +AG++I +NF S +M++  RPF + +WI++
Sbjct: 128 VVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRS 187

Query: 187 KIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAIS 246
                E  GTV  +G W  T I   D   +++PN  F+   V N  + T+ RI T + + 
Sbjct: 188 PDRNIE--GTVAEIG-WRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLR 247

Query: 247 HLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNP-ENQALLILISCFVKTSHFE 306
           + D  K+  IV  +R++L  +P ++Q    R+  L   N   + +L I++ CF KT+ + 
Sbjct: 248 YEDAAKVGVIVEAVREMLKNHPAIDQ----RQTLLVYFNQFADSSLNIMVYCFTKTTVWA 307

Query: 307 EYLCVKEAIILDLLRVIRHHRARLATPIRTM 337
           E+L  ++ + L ++ +++ H A  A P +T+
Sbjct: 308 EWLAAQQDVYLKIIDIVQSHGADFAFPSQTL 331

BLAST of Moc10g05770 vs. ExPASy Swiss-Prot
Match: P0AEB5 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) OX=83333 GN=ynaI PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 4.3e-22
Identity = 66/211 (31.28%), Postives = 116/211 (54.98%), Query Frame = 0

Query: 127 VAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIVNEWIQT 186
           V  V L+ E  G S    LT GG+G + + +AG++I +NF S +M++  RPF + +WI++
Sbjct: 128 VVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRS 187

Query: 187 KIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAIS 246
                E  GTV  +G W  T I   D   +++PN  F+   V N  + T+ RI T + + 
Sbjct: 188 PDRNIE--GTVAEIG-WRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLR 247

Query: 247 HLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNP-ENQALLILISCFVKTSHFE 306
           + D  K+  IV  +R++L  +P ++Q    R+  L   N   + +L I++ CF KT+ + 
Sbjct: 248 YEDAAKVGVIVEAVREMLKNHPAIDQ----RQTLLVYFNQFADSSLNIMVYCFTKTTVWA 307

Query: 307 EYLCVKEAIILDLLRVIRHHRARLATPIRTM 337
           E+L  ++ + L ++ +++ H A  A P +T+
Sbjct: 308 EWLAAQQDVYLKIIDIVQSHGADFAFPSQTL 331

BLAST of Moc10g05770 vs. ExPASy Swiss-Prot
Match: O66994 (Uncharacterized MscS family protein aq_812 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_812 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.3e-12
Identity = 64/240 (26.67%), Postives = 122/240 (50.83%), Query Frame = 0

Query: 98  KFFSETTESSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTL 157
           +FF +      A  +G  F  K + + V V   +  ++  G +    L + GL  + ++L
Sbjct: 130 EFFVKVGGKDFAEEVG-DFILKILKAFVVVIVGASLLQEWGVNIGAILASVGLLGLAVSL 189

Query: 158 AGREIFTNFLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVH 217
           A ++ F N LS ++I   +P  V E ++ K    +  G+VE +G  S T IR  D+  V 
Sbjct: 190 AAKDTFENILSGLIILLDKPVKVGETVKVK----DFMGSVEDIGLRS-TKIRTFDKSLVT 249

Query: 218 IPNHQFTINVVRNLSQKTHWRIKTHLAISH-LDVNKINNIVADMRKVLAKNPQVEQQRLH 277
           IPN     N V N +++   R++ ++ + +     ++ NI+ ++R++L ++P V +    
Sbjct: 250 IPNRDIVNNHVENFTRRNKRRVRFYIGVVYSTKREQLENILKEIRELLKEHPGVAKDEKF 309

Query: 278 RRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTM 337
             V+ EN    + +L ILI  +  T+ +EEYL + E I L ++ ++  + +  A P R++
Sbjct: 310 -YVYFENYG--DSSLNILIQYYANTNDYEEYLKIIEDINLKIMEIVEKNGSSFAFPSRSV 360

BLAST of Moc10g05770 vs. ExPASy TrEMBL
Match: A0A6J1C4S5 (mechanosensitive ion channel protein 2, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111008365 PE=4 SV=1)

HSP 1 Score: 1174.5 bits (3037), Expect = 0.0e+00
Identity = 608/608 (100.00%), Postives = 608/608 (100.00%), Query Frame = 0

Query: 1   MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 60
           MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL
Sbjct: 124 MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 183

Query: 61  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 120
           PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA
Sbjct: 184 PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 243

Query: 121 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 180
           VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV
Sbjct: 244 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 303

Query: 181 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 240
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK
Sbjct: 304 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 363

Query: 241 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 300
           THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK
Sbjct: 364 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 423

Query: 301 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 360
           TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV
Sbjct: 424 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 483

Query: 361 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 420
           NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE
Sbjct: 484 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 543

Query: 421 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 480
           VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT
Sbjct: 544 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 603

Query: 481 DNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKP 540
           DNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKP
Sbjct: 604 DNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKP 663

Query: 541 STLKPGVEENLILGVALDGSKRTLPIEEDMPSSPETVKDLAARWNGNGTNGATTPDKDTK 600
           STLKPGVEENLILGVALDGSKRTLPIEEDMPSSPETVKDLAARWNGNGTNGATTPDKDTK
Sbjct: 664 STLKPGVEENLILGVALDGSKRTLPIEEDMPSSPETVKDLAARWNGNGTNGATTPDKDTK 723

Query: 601 DQTPPPSE 609
           DQTPPPSE
Sbjct: 724 DQTPPPSE 731

BLAST of Moc10g05770 vs. ExPASy TrEMBL
Match: A0A6J1ES61 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436025 PE=4 SV=1)

HSP 1 Score: 972.2 bits (2512), Expect = 9.8e-280
Identity = 519/612 (84.80%), Postives = 555/612 (90.69%), Query Frame = 0

Query: 1   MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 60
           +FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW G  LICRALDPVVL
Sbjct: 124 IFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVVL 183

Query: 61  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 120
           PT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+ESSDARNMGFQFAWKA
Sbjct: 184 PTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWKA 243

Query: 121 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 180
           VYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPF+V
Sbjct: 244 VYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 303

Query: 181 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 240
           NEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIK
Sbjct: 304 NEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIK 363

Query: 241 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 300
           THLAISHLDV+KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK
Sbjct: 364 THLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 423

Query: 301 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 360
           TSHFEEYLCVKEAI LDLLRVIRHHRARLATPIRT+QKMHSDSDLESVPFSDSIFG GG 
Sbjct: 424 TSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGA 483

Query: 361 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 420
            LNRRMLMIEPPYKVYG+DRKQSHSRTSRTTGEQNGKPIARSSGD+KAAKE TPS+RKTE
Sbjct: 484 TLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTE 543

Query: 421 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 480
           V+ GE +DSD K H KVP S S+D  S+ESKHKSSSRS SSTNGI+ MP+SDAKTT SD 
Sbjct: 544 VKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDA 603

Query: 481 DNSFEDP-STKQSENGSGS-TKQNYKSNHPAVSLPEDVKK-TGGSSATSQPRIEGEQTPV 540
           DNS +D  S+K+S+N SGS  KQNYK  H +VS  EDVKK  G +S+ SQPRIEG+QT  
Sbjct: 604 DNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT-- 663

Query: 541 LKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPET---VKDL-AARWNGNGTNGAT 600
             PST KPGVEEN+ILGVALDGSKRTLPIE+D PSSP+T    KDL AA  NGNG  GAT
Sbjct: 664 TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGAT 723

Query: 601 TPDKDTKDQTPP 606
           TPDK+TK  +PP
Sbjct: 724 TPDKETKRLSPP 731

BLAST of Moc10g05770 vs. ExPASy TrEMBL
Match: A0A6J1ES66 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111436025 PE=4 SV=1)

HSP 1 Score: 972.2 bits (2512), Expect = 9.8e-280
Identity = 519/612 (84.80%), Postives = 555/612 (90.69%), Query Frame = 0

Query: 1   MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 60
           +FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW G  LICRALDPVVL
Sbjct: 9   IFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVVL 68

Query: 61  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 120
           PT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+ESSDARNMGFQFAWKA
Sbjct: 69  PTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWKA 128

Query: 121 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 180
           VYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPF+V
Sbjct: 129 VYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 188

Query: 181 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 240
           NEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIK
Sbjct: 189 NEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIK 248

Query: 241 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 300
           THLAISHLDV+KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK
Sbjct: 249 THLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 308

Query: 301 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 360
           TSHFEEYLCVKEAI LDLLRVIRHHRARLATPIRT+QKMHSDSDLESVPFSDSIFG GG 
Sbjct: 309 TSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGA 368

Query: 361 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 420
            LNRRMLMIEPPYKVYG+DRKQSHSRTSRTTGEQNGKPIARSSGD+KAAKE TPS+RKTE
Sbjct: 369 TLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTE 428

Query: 421 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 480
           V+ GE +DSD K H KVP S S+D  S+ESKHKSSSRS SSTNGI+ MP+SDAKTT SD 
Sbjct: 429 VKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDA 488

Query: 481 DNSFEDP-STKQSENGSGS-TKQNYKSNHPAVSLPEDVKK-TGGSSATSQPRIEGEQTPV 540
           DNS +D  S+K+S+N SGS  KQNYK  H +VS  EDVKK  G +S+ SQPRIEG+QT  
Sbjct: 489 DNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT-- 548

Query: 541 LKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPET---VKDL-AARWNGNGTNGAT 600
             PST KPGVEEN+ILGVALDGSKRTLPIE+D PSSP+T    KDL AA  NGNG  GAT
Sbjct: 549 TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGAT 608

Query: 601 TPDKDTKDQTPP 606
           TPDK+TK  +PP
Sbjct: 609 TPDKETKRLSPP 616

BLAST of Moc10g05770 vs. ExPASy TrEMBL
Match: A0A6J1EN69 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436025 PE=4 SV=1)

HSP 1 Score: 972.2 bits (2512), Expect = 9.8e-280
Identity = 519/612 (84.80%), Postives = 555/612 (90.69%), Query Frame = 0

Query: 1   MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 60
           +FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW G  LICRALDPVVL
Sbjct: 104 IFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVVL 163

Query: 61  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 120
           PT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+ESSDARNMGFQFAWKA
Sbjct: 164 PTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWKA 223

Query: 121 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 180
           VYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPF+V
Sbjct: 224 VYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 283

Query: 181 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 240
           NEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIK
Sbjct: 284 NEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIK 343

Query: 241 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 300
           THLAISHLDV+KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK
Sbjct: 344 THLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 403

Query: 301 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 360
           TSHFEEYLCVKEAI LDLLRVIRHHRARLATPIRT+QKMHSDSDLESVPFSDSIFG GG 
Sbjct: 404 TSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGA 463

Query: 361 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 420
            LNRRMLMIEPPYKVYG+DRKQSHSRTSRTTGEQNGKPIARSSGD+KAAKE TPS+RKTE
Sbjct: 464 TLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTE 523

Query: 421 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 480
           V+ GE +DSD K H KVP S S+D  S+ESKHKSSSRS SSTNGI+ MP+SDAKTT SD 
Sbjct: 524 VKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDA 583

Query: 481 DNSFEDP-STKQSENGSGS-TKQNYKSNHPAVSLPEDVKK-TGGSSATSQPRIEGEQTPV 540
           DNS +D  S+K+S+N SGS  KQNYK  H +VS  EDVKK  G +S+ SQPRIEG+QT  
Sbjct: 584 DNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT-- 643

Query: 541 LKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPET---VKDL-AARWNGNGTNGAT 600
             PST KPGVEEN+ILGVALDGSKRTLPIE+D PSSP+T    KDL AA  NGNG  GAT
Sbjct: 644 TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGAT 703

Query: 601 TPDKDTKDQTPP 606
           TPDK+TK  +PP
Sbjct: 704 TPDKETKRLSPP 711

BLAST of Moc10g05770 vs. ExPASy TrEMBL
Match: A0A6J1EMU6 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111436025 PE=4 SV=1)

HSP 1 Score: 972.2 bits (2512), Expect = 9.8e-280
Identity = 519/612 (84.80%), Postives = 555/612 (90.69%), Query Frame = 0

Query: 1   MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 60
           +FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW G  LICRALDPVVL
Sbjct: 43  IFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDPVVL 102

Query: 61  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 120
           PT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+ESSDARNMGFQFAWKA
Sbjct: 103 PTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFAWKA 162

Query: 121 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 180
           VYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPF+V
Sbjct: 163 VYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 222

Query: 181 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 240
           NEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIK
Sbjct: 223 NEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIK 282

Query: 241 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 300
           THLAISHLDV+KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK
Sbjct: 283 THLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 342

Query: 301 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 360
           TSHFEEYLCVKEAI LDLLRVIRHHRARLATPIRT+QKMHSDSDLESVPFSDSIFG GG 
Sbjct: 343 TSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGA 402

Query: 361 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 420
            LNRRMLMIEPPYKVYG+DRKQSHSRTSRTTGEQNGKPIARSSGD+KAAKE TPS+RKTE
Sbjct: 403 TLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTE 462

Query: 421 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 480
           V+ GE +DSD K H KVP S S+D  S+ESKHKSSSRS SSTNGI+ MP+SDAKTT SD 
Sbjct: 463 VKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDA 522

Query: 481 DNSFEDP-STKQSENGSGS-TKQNYKSNHPAVSLPEDVKK-TGGSSATSQPRIEGEQTPV 540
           DNS +D  S+K+S+N SGS  KQNYK  H +VS  EDVKK  G +S+ SQPRIEG+QT  
Sbjct: 523 DNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQT-- 582

Query: 541 LKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPET---VKDL-AARWNGNGTNGAT 600
             PST KPGVEEN+ILGVALDGSKRTLPIE+D PSSP+T    KDL AA  NGNG  GAT
Sbjct: 583 TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGAT 642

Query: 601 TPDKDTKDQTPP 606
           TPDK+TK  +PP
Sbjct: 643 TPDKETKRLSPP 650

BLAST of Moc10g05770 vs. TAIR 10
Match: AT5G10490.1 (MSCS-like 2 )

HSP 1 Score: 655.6 bits (1690), Expect = 3.9e-188
Identity = 364/608 (59.87%), Postives = 431/608 (70.89%), Query Frame = 0

Query: 1   MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 60
           +F++WGL PF R  R++LL+ +DN WKKS TY VMT Y+QPLLLW G   ICRALDPVVL
Sbjct: 121 VFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVL 180

Query: 61  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 120
           PT+ S++VK R+LNFVRSLSTVLAFAYCLSS+I+Q QK FSET+  SD RNMGFQFA KA
Sbjct: 181 PTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKA 240

Query: 121 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 180
           +YSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRPF++
Sbjct: 241 LYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVL 300

Query: 181 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 240
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREA+HIPNH+FT+NVVRNL+QKTHWRIK
Sbjct: 301 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIK 360

Query: 241 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 300
           THLAISHLDVNKINNIVADMRKVLAKNP VEQQRLHRRVFLENV PENQAL ILISCFVK
Sbjct: 361 THLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVK 420

Query: 301 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 360
           TSH EEYL VKEAI+LDLLRVI HHRARLATPIRT++KM++++D+E+ PF +S++  GGV
Sbjct: 421 TSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGESMY--GGV 480

Query: 361 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 420
              R +++IEP YK+ GED+ +S +R ++ T EQ  K      G +  +KE +  D K  
Sbjct: 481 TSRRPLMLIEPAYKINGEDKSKSQNRAAKPTAEQENK------GSNPKSKETSSPDLKAN 540

Query: 421 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 480
           V+ GE   SDT K P+                                     +T     
Sbjct: 541 VKVGESPVSDTNKVPE-------------------------------------ETVAKPV 600

Query: 481 DNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKP 540
             +   P T +    SG+ K                K++GG+  +++       T     
Sbjct: 601 IKAVSKPPTPKDTETSGTEKPK-------------AKRSGGTIKSTKTDETDSSTSSASR 660

Query: 541 STLKPGVEENLILGVALDGSKRTLPIEEDMPSSP-ETVKDLAARWNGNGTNGATTPDKDT 600
           STL    EEN++LGVAL+GSKRTLPIEE++ S P ET          +G NG    DK+ 
Sbjct: 661 STL----EENIVLGVALEGSKRTLPIEEEIHSPPMETDAKELTGARRSGGNGPLVADKEQ 666

Query: 601 KDQTPPPS 608
           KD    P+
Sbjct: 721 KDSQSQPN 666

BLAST of Moc10g05770 vs. TAIR 10
Match: AT5G10490.2 (MSCS-like 2 )

HSP 1 Score: 655.6 bits (1690), Expect = 3.9e-188
Identity = 364/608 (59.87%), Postives = 431/608 (70.89%), Query Frame = 0

Query: 1   MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 60
           +F++WGL PF R  R++LL+ +DN WKKS TY VMT Y+QPLLLW G   ICRALDPVVL
Sbjct: 93  VFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVL 152

Query: 61  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 120
           PT+ S++VK R+LNFVRSLSTVLAFAYCLSS+I+Q QK FSET+  SD RNMGFQFA KA
Sbjct: 153 PTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKA 212

Query: 121 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 180
           +YSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRPF++
Sbjct: 213 LYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVL 272

Query: 181 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 240
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREA+HIPNH+FT+NVVRNL+QKTHWRIK
Sbjct: 273 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIK 332

Query: 241 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 300
           THLAISHLDVNKINNIVADMRKVLAKNP VEQQRLHRRVFLENV PENQAL ILISCFVK
Sbjct: 333 THLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVK 392

Query: 301 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 360
           TSH EEYL VKEAI+LDLLRVI HHRARLATPIRT++KM++++D+E+ PF +S++  GGV
Sbjct: 393 TSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGESMY--GGV 452

Query: 361 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 420
              R +++IEP YK+ GED+ +S +R ++ T EQ  K      G +  +KE +  D K  
Sbjct: 453 TSRRPLMLIEPAYKINGEDKSKSQNRAAKPTAEQENK------GSNPKSKETSSPDLKAN 512

Query: 421 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 480
           V+ GE   SDT K P+                                     +T     
Sbjct: 513 VKVGESPVSDTNKVPE-------------------------------------ETVAKPV 572

Query: 481 DNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKP 540
             +   P T +    SG+ K                K++GG+  +++       T     
Sbjct: 573 IKAVSKPPTPKDTETSGTEKPK-------------AKRSGGTIKSTKTDETDSSTSSASR 632

Query: 541 STLKPGVEENLILGVALDGSKRTLPIEEDMPSSP-ETVKDLAARWNGNGTNGATTPDKDT 600
           STL    EEN++LGVAL+GSKRTLPIEE++ S P ET          +G NG    DK+ 
Sbjct: 633 STL----EENIVLGVALEGSKRTLPIEEEIHSPPMETDAKELTGARRSGGNGPLVADKEQ 638

Query: 601 KDQTPPPS 608
           KD    P+
Sbjct: 693 KDSQSQPN 638

BLAST of Moc10g05770 vs. TAIR 10
Match: AT5G10490.3 (MSCS-like 2 )

HSP 1 Score: 655.6 bits (1690), Expect = 3.9e-188
Identity = 364/608 (59.87%), Postives = 431/608 (70.89%), Query Frame = 0

Query: 1   MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 60
           +F++WGL PF R  R++LL+ +DN WKKS TY VMT Y+QPLLLW G   ICRALDPVVL
Sbjct: 119 VFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVL 178

Query: 61  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 120
           PT+ S++VK R+LNFVRSLSTVLAFAYCLSS+I+Q QK FSET+  SD RNMGFQFA KA
Sbjct: 179 PTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKA 238

Query: 121 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 180
           +YSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRPF++
Sbjct: 239 LYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVL 298

Query: 181 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 240
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREA+HIPNH+FT+NVVRNL+QKTHWRIK
Sbjct: 299 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIK 358

Query: 241 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 300
           THLAISHLDVNKINNIVADMRKVLAKNP VEQQRLHRRVFLENV PENQAL ILISCFVK
Sbjct: 359 THLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVK 418

Query: 301 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 360
           TSH EEYL VKEAI+LDLLRVI HHRARLATPIRT++KM++++D+E+ PF +S++  GGV
Sbjct: 419 TSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGESMY--GGV 478

Query: 361 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 420
              R +++IEP YK+ GED+ +S +R ++ T EQ  K      G +  +KE +  D K  
Sbjct: 479 TSRRPLMLIEPAYKINGEDKSKSQNRAAKPTAEQENK------GSNPKSKETSSPDLKAN 538

Query: 421 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 480
           V+ GE   SDT K P+                                     +T     
Sbjct: 539 VKVGESPVSDTNKVPE-------------------------------------ETVAKPV 598

Query: 481 DNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKP 540
             +   P T +    SG+ K                K++GG+  +++       T     
Sbjct: 599 IKAVSKPPTPKDTETSGTEKPK-------------AKRSGGTIKSTKTDETDSSTSSASR 658

Query: 541 STLKPGVEENLILGVALDGSKRTLPIEEDMPSSP-ETVKDLAARWNGNGTNGATTPDKDT 600
           STL    EEN++LGVAL+GSKRTLPIEE++ S P ET          +G NG    DK+ 
Sbjct: 659 STL----EENIVLGVALEGSKRTLPIEEEIHSPPMETDAKELTGARRSGGNGPLVADKEQ 664

Query: 601 KDQTPPPS 608
           KD    P+
Sbjct: 719 KDSQSQPN 664

BLAST of Moc10g05770 vs. TAIR 10
Match: AT1G58200.1 (MSCS-like 3 )

HSP 1 Score: 575.1 bits (1481), Expect = 6.7e-164
Identity = 327/580 (56.38%), Postives = 406/580 (70.00%), Query Frame = 0

Query: 2   FAVWGLGPFLRYTRSLLLH-GSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 61
           FA WGL P LR  R+ L   G+D N +KS T  ++  YLQPLLLW+G  L+CR LDP+VL
Sbjct: 123 FATWGLRPLLRLARTTLFEKGNDANSQKSSTQYIVVSYLQPLLLWSGAILLCRTLDPIVL 182

Query: 62  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 121
           P+   Q +KQR+L F RS+STVLAF+ CLSS+++Q QKFF ET   +D RNMGF FA KA
Sbjct: 183 PSSAGQAIKQRLLIFARSISTVLAFSCCLSSLLQQVQKFFMETNNPADTRNMGFSFAGKA 242

Query: 122 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 181
           VY+A WVAA SLFMELLGFSTQKWLTAGGLGTVLLTLAGREI TNFLSS+MIHATRPF++
Sbjct: 243 VYTAAWVAAASLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATRPFVL 302

Query: 182 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 241
           NEWIQTKI GYEVSGTVE VGWWSPTIIRG+DREAVHIPNHQF++N+VRNL+QKTHWRIK
Sbjct: 303 NEWIQTKIGGYEVSGTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWRIK 362

Query: 242 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 301
           THLAISHLDV+KINNIVADMRKVL+KNPQ+EQQ++HRRVFLE+++PENQAL ILISCFVK
Sbjct: 363 THLAISHLDVSKINNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALRILISCFVK 422

Query: 302 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 361
           TS FEEYLCVKEA++LDLL VIRHH ARLATPIRT+Q+M +++++++  FSD +F +   
Sbjct: 423 TSRFEEYLCVKEAVLLDLLTVIRHHGARLATPIRTVQRMRNEAEVDTAGFSDIVFNQAA- 482

Query: 362 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 421
            +NRR ++IEP YK+  +D  +S    S + G+++  P  +S    +   +  PS+ K E
Sbjct: 483 -MNRRYMLIEPSYKINSDDNSKS---PSPSPGQKSPSPGQKS---EERDLQEEPSETKAE 542

Query: 422 V-RTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSS------RSASSTNGISGMPSSDA 481
               G    S+ KK  +     S+ N+  +    S+S      +S        G P    
Sbjct: 543 TENNGSVPVSNAKKENQKAALGSNSNTGTKGSSTSTSDQPVAQKSEEKKKESVGDPHKAE 602

Query: 482 KTTRSDTDNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGE 541
           K   SD + + E   T +S+   GS K N +S            + GG S TS       
Sbjct: 603 KDEVSDDEATIE--QTLKSKAKQGSEKNNGESK----------ARDGGGSGTSSL----- 662

Query: 542 QTPVLKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSS 574
                        +EENL+LGVALDGSKRTLPI+E+  +S
Sbjct: 663 -------------LEENLVLGVALDGSKRTLPIDEEHKAS 664

BLAST of Moc10g05770 vs. TAIR 10
Match: AT1G58200.2 (MSCS-like 3 )

HSP 1 Score: 575.1 bits (1481), Expect = 6.7e-164
Identity = 327/580 (56.38%), Postives = 406/580 (70.00%), Query Frame = 0

Query: 2   FAVWGLGPFLRYTRSLLLH-GSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 61
           FA WGL P LR  R+ L   G+D N +KS T  ++  YLQPLLLW+G  L+CR LDP+VL
Sbjct: 123 FATWGLRPLLRLARTTLFEKGNDANSQKSSTQYIVVSYLQPLLLWSGAILLCRTLDPIVL 182

Query: 62  PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 121
           P+   Q +KQR+L F RS+STVLAF+ CLSS+++Q QKFF ET   +D RNMGF FA KA
Sbjct: 183 PSSAGQAIKQRLLIFARSISTVLAFSCCLSSLLQQVQKFFMETNNPADTRNMGFSFAGKA 242

Query: 122 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 181
           VY+A WVAA SLFMELLGFSTQKWLTAGGLGTVLLTLAGREI TNFLSS+MIHATRPF++
Sbjct: 243 VYTAAWVAAASLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATRPFVL 302

Query: 182 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 241
           NEWIQTKI GYEVSGTVE VGWWSPTIIRG+DREAVHIPNHQF++N+VRNL+QKTHWRIK
Sbjct: 303 NEWIQTKIGGYEVSGTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWRIK 362

Query: 242 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 301
           THLAISHLDV+KINNIVADMRKVL+KNPQ+EQQ++HRRVFLE+++PENQAL ILISCFVK
Sbjct: 363 THLAISHLDVSKINNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALRILISCFVK 422

Query: 302 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 361
           TS FEEYLCVKEA++LDLL VIRHH ARLATPIRT+Q+M +++++++  FSD +F +   
Sbjct: 423 TSRFEEYLCVKEAVLLDLLTVIRHHGARLATPIRTVQRMRNEAEVDTAGFSDIVFNQAA- 482

Query: 362 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 421
            +NRR ++IEP YK+  +D  +S    S + G+++  P  +S    +   +  PS+ K E
Sbjct: 483 -MNRRYMLIEPSYKINSDDNSKS---PSPSPGQKSPSPGQKS---EERDLQEEPSETKAE 542

Query: 422 V-RTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSS------RSASSTNGISGMPSSDA 481
               G    S+ KK  +     S+ N+  +    S+S      +S        G P    
Sbjct: 543 TENNGSVPVSNAKKENQKAALGSNSNTGTKGSSTSTSDQPVAQKSEEKKKESVGDPHKAE 602

Query: 482 KTTRSDTDNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGE 541
           K   SD + + E   T +S+   GS K N +S            + GG S TS       
Sbjct: 603 KDEVSDDEATIE--QTLKSKAKQGSEKNNGESK----------ARDGGGSGTSSL----- 662

Query: 542 QTPVLKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSS 574
                        +EENL+LGVALDGSKRTLPI+E+  +S
Sbjct: 663 -------------LEENLVLGVALDGSKRTLPIDEEHKAS 664