Moc03g24910 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc03g24910
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionberberine bridge enzyme-like 13
Locationchr3: 17885666 .. 17887132 (-)
RNA-Seq ExpressionMoc03g24910
SyntenyMoc03g24910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGTCTCCAATTACCTCTTTAGCCTATACTTTTGTTGTCATATCCTTTCTATGGGTAGCTTCTGCTTCCAACTATGAAGATTTTCTCCATTGTCTTTCTCTTCATTCTCCCAACAGTTCTTCAATTTCAAAGATTATTTACACTCCAACTGATCCTTCGTACTCTTTGATTATGAACTTCTCTACCCATAACTTAGGATTGAGGACTGCAAAATCCTTTAAGCCTCAAGCAATAGTGACGCCATTGCAAGTTTCCCACATTCAATTATCCATCATTTGCGCCAAAAAACACGGCTTCCAGATACGAACGCGAAGCGGTGGCCATGATTACGAGCGCCTTTCCTCTATCTCCTATGACGACATTCCTTTCGTTGTTGTTGATATGATAAACATTCGGTCGATCGATGTCAATGTGGAAAAAAACGTTGCATGGGTGCAATCTGGAGCCACCATCGGGGAACTGTACTATAGAATTGCACAGAAAAGCAAAACCCTAGGGTTCCCAGCTGGGCTATGCCCGACAGTTGGTGTTGGTGGGCATTTCAGTGGCGGTGGTTATGGTATGATGCTACGAAAATTTGGACTCGCTGCCGATAATGTGATCGATGCTTACTTCGTTGATGTAAACGGTAAACTCCACAATCGAAAATCGATGGGAGAGGATGTGTTTTGGGCCATTAGAGGAGGAGGAGGAGCAAGCTTTGACATTATTGTTGCATGGAAAATTAAGCTCGTCCAAGTTCCACCAGTGGTGACTGTTTTTGAAATCACAAGAAACTTGGAACAAAATGCTACCAATTTAGTCCATCGTTGGCAATACGTGGCCAACAAACAAGATGACAAACTTCTCATCAAAATAACATTAAGAGGCATGAACTCAAACAATCATCAAAATGGAAAAAGAACACGAGAAACATCTTTCGACTCCTTGTTCCTCGGACAAGCCGAAGAGCTTCTTCCCATCATGCAGAATACTTTCCCGGAGCTGGGGCTGACGAAAGAAGATTGTATAGAAATGAGTTGGATTGAATCCGTTCTCCATTTTGCCGGCTTCCGTGGAGGACAGCCGTTAGATGTCCTTCTCGATAGGAATGTGGCTTTTGAAGCCAATGGAAAAGCCAAAACCGACTATGTAAATGAGCCAATCCCCATAGTTGGGCTTCAGGGTATATGGGAATTGTTCAACGAAGAAGAAGTTGAATTTGCACAAATATTTTTTATCCCTTACGGTGGAAAAATGAATGAGATTCCTGAATCAGAGATTCCATTTCCGCATAGAGCTGGGAATCTTTACAAGATTCATTATTCGGTGGTTTGGACAGAAGAAGACGAAGATACTACACAAAAACATATAAATTGGATCCGTAAGCTTGAAAGTTACATGACTCCTTTCAAGGGCCGCGTATATCAATTATCGAGACTTGGATATAGGAATAAACAACAAGAATGGATACACGAGCTATGA

mRNA sequence

ATGAAGTCTCCAATTACCTCTTTAGCCTATACTTTTGTTGTCATATCCTTTCTATGGGTAGCTTCTGCTTCCAACTATGAAGATTTTCTCCATTGTCTTTCTCTTCATTCTCCCAACAGTTCTTCAATTTCAAAGATTATTTACACTCCAACTGATCCTTCGTACTCTTTGATTATGAACTTCTCTACCCATAACTTAGGATTGAGGACTGCAAAATCCTTTAAGCCTCAAGCAATAGTGACGCCATTGCAAGTTTCCCACATTCAATTATCCATCATTTGCGCCAAAAAACACGGCTTCCAGATACGAACGCGAAGCGGTGGCCATGATTACGAGCGCCTTTCCTCTATCTCCTATGACGACATTCCTTTCGTTGTTGTTGATATGATAAACATTCGGTCGATCGATGTCAATGTGGAAAAAAACGTTGCATGGGTGCAATCTGGAGCCACCATCGGGGAACTGTACTATAGAATTGCACAGAAAAGCAAAACCCTAGGGTTCCCAGCTGGGCTATGCCCGACAGTTGGTGTTGGTGGGCATTTCAGTGGCGGTGGTTATGGTATGATGCTACGAAAATTTGGACTCGCTGCCGATAATGTGATCGATGCTTACTTCGTTGATGTAAACGGTAAACTCCACAATCGAAAATCGATGGGAGAGGATGTGTTTTGGGCCATTAGAGGAGGAGGAGGAGCAAGCTTTGACATTATTGTTGCATGGAAAATTAAGCTCGTCCAAGTTCCACCAGTGGTGACTGTTTTTGAAATCACAAGAAACTTGGAACAAAATGCTACCAATTTAGTCCATCGTTGGCAATACGTGGCCAACAAACAAGATGACAAACTTCTCATCAAAATAACATTAAGAGGCATGAACTCAAACAATCATCAAAATGGAAAAAGAACACGAGAAACATCTTTCGACTCCTTGTTCCTCGGACAAGCCGAAGAGCTTCTTCCCATCATGCAGAATACTTTCCCGGAGCTGGGGCTGACGAAAGAAGATTGTATAGAAATGAGTTGGATTGAATCCGTTCTCCATTTTGCCGGCTTCCGTGGAGGACAGCCGTTAGATGTCCTTCTCGATAGGAATGTGGCTTTTGAAGCCAATGGAAAAGCCAAAACCGACTATGTAAATGAGCCAATCCCCATAGTTGGGCTTCAGGGTATATGGGAATTGTTCAACGAAGAAGAAGTTGAATTTGCACAAATATTTTTTATCCCTTACGGTGGAAAAATGAATGAGATTCCTGAATCAGAGATTCCATTTCCGCATAGAGCTGGGAATCTTTACAAGATTCATTATTCGGTGGTTTGGACAGAAGAAGACGAAGATACTACACAAAAACATATAAATTGGATCCGTAAGCTTGAAAGTTACATGACTCCTTTCAAGGGCCGCGTATATCAATTATCGAGACTTGGATATAGGAATAAACAACAAGAATGGATACACGAGCTATGA

Coding sequence (CDS)

ATGAAGTCTCCAATTACCTCTTTAGCCTATACTTTTGTTGTCATATCCTTTCTATGGGTAGCTTCTGCTTCCAACTATGAAGATTTTCTCCATTGTCTTTCTCTTCATTCTCCCAACAGTTCTTCAATTTCAAAGATTATTTACACTCCAACTGATCCTTCGTACTCTTTGATTATGAACTTCTCTACCCATAACTTAGGATTGAGGACTGCAAAATCCTTTAAGCCTCAAGCAATAGTGACGCCATTGCAAGTTTCCCACATTCAATTATCCATCATTTGCGCCAAAAAACACGGCTTCCAGATACGAACGCGAAGCGGTGGCCATGATTACGAGCGCCTTTCCTCTATCTCCTATGACGACATTCCTTTCGTTGTTGTTGATATGATAAACATTCGGTCGATCGATGTCAATGTGGAAAAAAACGTTGCATGGGTGCAATCTGGAGCCACCATCGGGGAACTGTACTATAGAATTGCACAGAAAAGCAAAACCCTAGGGTTCCCAGCTGGGCTATGCCCGACAGTTGGTGTTGGTGGGCATTTCAGTGGCGGTGGTTATGGTATGATGCTACGAAAATTTGGACTCGCTGCCGATAATGTGATCGATGCTTACTTCGTTGATGTAAACGGTAAACTCCACAATCGAAAATCGATGGGAGAGGATGTGTTTTGGGCCATTAGAGGAGGAGGAGGAGCAAGCTTTGACATTATTGTTGCATGGAAAATTAAGCTCGTCCAAGTTCCACCAGTGGTGACTGTTTTTGAAATCACAAGAAACTTGGAACAAAATGCTACCAATTTAGTCCATCGTTGGCAATACGTGGCCAACAAACAAGATGACAAACTTCTCATCAAAATAACATTAAGAGGCATGAACTCAAACAATCATCAAAATGGAAAAAGAACACGAGAAACATCTTTCGACTCCTTGTTCCTCGGACAAGCCGAAGAGCTTCTTCCCATCATGCAGAATACTTTCCCGGAGCTGGGGCTGACGAAAGAAGATTGTATAGAAATGAGTTGGATTGAATCCGTTCTCCATTTTGCCGGCTTCCGTGGAGGACAGCCGTTAGATGTCCTTCTCGATAGGAATGTGGCTTTTGAAGCCAATGGAAAAGCCAAAACCGACTATGTAAATGAGCCAATCCCCATAGTTGGGCTTCAGGGTATATGGGAATTGTTCAACGAAGAAGAAGTTGAATTTGCACAAATATTTTTTATCCCTTACGGTGGAAAAATGAATGAGATTCCTGAATCAGAGATTCCATTTCCGCATAGAGCTGGGAATCTTTACAAGATTCATTATTCGGTGGTTTGGACAGAAGAAGACGAAGATACTACACAAAAACATATAAATTGGATCCGTAAGCTTGAAAGTTACATGACTCCTTTCAAGGGCCGCGTATATCAATTATCGAGACTTGGATATAGGAATAAACAACAAGAATGGATACACGAGCTATGA

Protein sequence

MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMNFSTHNLGLRTAKSFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYDDIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQVPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYRNKQQEWIHEL
Homology
BLAST of Moc03g24910 vs. NCBI nr
Match: XP_022152545.1 (berberine bridge enzyme-like 13 [Momordica charantia])

HSP 1 Score: 940.6 bits (2430), Expect = 5.2e-270
Identity = 461/479 (96.24%), Postives = 467/479 (97.49%), Query Frame = 0

Query: 1   MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMN 60
           MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMN
Sbjct: 1   MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMN 60

Query: 61  FSTHNLGLRTAKSFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYD 120
           FSTHNLGLRTAKSFKPQAIVTPLQVSHIQ SIICAKKHGFQIRTRSGGHDYERLSSISYD
Sbjct: 61  FSTHNLGLRTAKSFKPQAIVTPLQVSHIQXSIICAKKHGFQIRTRSGGHDYERLSSISYD 120

Query: 121 DIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGG 180
           DIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGG
Sbjct: 121 DIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGG 180

Query: 181 HFSGGGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVA 240
           HFSGGGYGMMLRKFGLAA+NVIDAYFVDVNGKLHNRKS GEDVFWAIRGGGGASF IIVA
Sbjct: 181 HFSGGGYGMMLRKFGLAABNVIDAYFVDVNGKLHNRKSXGEDVFWAIRGGGGASFXIIVA 240

Query: 241 WKIKLVQVPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNG 300
           WKIKLVQ PPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNG
Sbjct: 241 WKIKLVQXPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNG 300

Query: 301 KRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDV 360
           KRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQ LDV
Sbjct: 301 KRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQXLDV 360

Query: 361 LLDRNVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPES 420
           LLDRNVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPES
Sbjct: 361 LLDRNVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPES 420

Query: 421 EIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYRN 480
           EIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPF  +  + + + YR+
Sbjct: 421 EIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRD 479

BLAST of Moc03g24910 vs. NCBI nr
Match: XP_022152423.1 (berberine bridge enzyme-like 15 [Momordica charantia])

HSP 1 Score: 743.8 bits (1919), Expect = 9.4e-211
Identity = 366/480 (76.25%), Postives = 420/480 (87.50%), Query Frame = 0

Query: 1   MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMN 60
           MKSPITSLAY FVV+SF+WVASASNYEDFL CLS HSPN+SSISKI+YTP D SY  I+N
Sbjct: 1   MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 60

Query: 61  FSTHNLGLRTAKSFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYD 120
           FS  NL   TAK+ KPQ IVTPLQVSHIQ SIICAK +GFQIRTRSGGHDYE LS +S D
Sbjct: 61  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVS-D 120

Query: 121 DIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGG 180
           ++PFVV+D+ N+RSIDV+VEKNVAWVQSGATIGELYYRIA+KSKTLGFPAGLC TVGVGG
Sbjct: 121 NVPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGG 180

Query: 181 HFSGGGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVA 240
           HFSGGGYG+MLRKFGLAADNVIDAYF+DVNG L +RKSMGEDVFWAIRGGGGASF II+A
Sbjct: 181 HFSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILA 240

Query: 241 WKIKLVQVPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGM-NSNNHQN 300
           WKIKL+ VPPVVTVF ITRNLEQNATNL+H+WQYVA+KQDD+LLI++ L GM NS+N+QN
Sbjct: 241 WKIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQN 300

Query: 301 GKRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLD 360
            K+T E SF+SLFLGQ +ELLPIMQ TFPELGLTKEDCIEMSWIE+VL+FAGF+ GQPL+
Sbjct: 301 XKKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQ-GQPLN 360

Query: 361 VLLDRNVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPE 420
           VLLDR++A++   K K+DYVNEPIP  G++GIWELFNEEEV+FAQ+  IPYGGKM+EI E
Sbjct: 361 VLLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISE 420

Query: 421 SEIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYRN 480
            EIPFPHR GNLYKIHY+V+   E EDT Q+ I+WIRKL SYMTPF  +  + + + YR+
Sbjct: 421 XEIPFPHRVGNLYKIHYAVL-LGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRD 477

BLAST of Moc03g24910 vs. NCBI nr
Match: XP_022152425.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 724.2 bits (1868), Expect = 7.7e-205
Identity = 354/479 (73.90%), Postives = 406/479 (84.76%), Query Frame = 0

Query: 1   MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMN 60
           MKSPI SLAY FVV+SF  VASA NYEDFLHCLS H PNSSSISKIIYTPT PSYSL++N
Sbjct: 1   MKSPIISLAYIFVVLSFARVASACNYEDFLHCLSFHFPNSSSISKIIYTPTHPSYSLVLN 60

Query: 61  FSTHNLGLRTAKSFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYD 120
           FS  NL    AK+ KPQ IVTPL+ SH+Q SIICAK +G QIRTRSGGHDYE LS +S  
Sbjct: 61  FSIQNLRFLGAKTPKPQIIVTPLEASHVQASIICAKNNGLQIRTRSGGHDYEGLSYVS-- 120

Query: 121 DIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGG 180
           ++PFVVVD+ N+RSIDV+VE+ VAWVQSGATIGELYYRIA+KSKTLGFPAGLCPTVG+GG
Sbjct: 121 NVPFVVVDLTNLRSIDVDVEQKVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGG 180

Query: 181 HFSGGGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVA 240
           HFSGGGYG+MLRKFGLAADNVIDAYF+DVNGKLH+RKSMGEDVFWAIRGGGGASF I++A
Sbjct: 181 HFSGGGYGVMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLA 240

Query: 241 WKIKLVQVPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNG 300
           WKIKL+QVP +VTVF ITRNLEQNATNL++RWQYVANKQDD+L+IKI L G NSNNH+NG
Sbjct: 241 WKIKLLQVPXLVTVFTITRNLEQNATNLIYRWQYVANKQDDRLIIKIILMGTNSNNHRNG 300

Query: 301 KRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDV 360
           KRT E  F+SLFLG+ E+LLPIMQ TFPELGL KEDCIEMSWIESVL+FA F  GQPL+V
Sbjct: 301 KRTIEAFFNSLFLGKVEDLLPIMQKTFPELGLMKEDCIEMSWIESVLYFAEFPRGQPLNV 360

Query: 361 LLDRNVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPES 420
           LLDR   F+ + K K+DYVNEP+P  GL+GIWELFNE+E +  Q+F IPYGGKMNEI ES
Sbjct: 361 LLDRRSTFKGSFKGKSDYVNEPVPKAGLEGIWELFNEKEAKMVQMFLIPYGGKMNEISES 420

Query: 421 EIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYRN 480
           EIPFPHRAGNLY+IHYS+    ++E   Q++  WIRK  SYM PF  +  + + + YR+
Sbjct: 421 EIPFPHRAGNLYQIHYSIYCGGDEE---QRNKIWIRKFYSYMAPFVSKNPRAAYINYRD 474

BLAST of Moc03g24910 vs. NCBI nr
Match: XP_022954564.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 719.9 bits (1857), Expect = 1.5e-203
Identity = 347/481 (72.14%), Postives = 405/481 (84.20%), Query Frame = 0

Query: 1   MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMN 60
           MK PIT   YTFVV+SF W ASA N+EDFLHCLSLHSPNSSSISKIIYTP D SYS ++N
Sbjct: 1   MKPPITLFVYTFVVLSFAWAASAHNHEDFLHCLSLHSPNSSSISKIIYTPNDSSYSSVLN 60

Query: 61  FSTHNLGLRTAKSFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYD 120
           FS HNL   T ++ KPQ IVTPLQV+ I+ SIICAK  GFQIRTRSGGHDYE LS +S  
Sbjct: 61  FSIHNLRFATPQTPKPQVIVTPLQVTEIKASIICAKTTGFQIRTRSGGHDYEGLSYVS-- 120

Query: 121 DIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGG 180
           D+PF+VVD+IN+RSI+V+ E NVAWVQSGATIGELYYRIA+KSKTLGFPAG+CPTVGVGG
Sbjct: 121 DVPFIVVDLINLRSINVDAENNVAWVQSGATIGELYYRIAKKSKTLGFPAGVCPTVGVGG 180

Query: 181 HFSGGGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVA 240
           HFSGGGYG+MLRKFGLA DNVIDAYF+DVNG LH+RKSMGEDVFWA+RGGGGASF I+VA
Sbjct: 181 HFSGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVA 240

Query: 241 WKIKLVQVPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNG 300
           WKIKL+QVPP+VTVF +TRNLEQNATNL+HRWQY+A+KQDD+L I+I +RG+NS N Q+G
Sbjct: 241 WKIKLLQVPPIVTVFTVTRNLEQNATNLIHRWQYIASKQDDRLFIRIIIRGVNSTNPQHG 300

Query: 301 -KRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLD 360
            +RT E +F+S+FLG++EELLPIMQNTFPELGLTKEDCIEMSWIES+L+FAGF  GQPL+
Sbjct: 301 SQRTLEAAFNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLN 360

Query: 361 VLLDRNVAFEAN-GKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIP 420
           VLLDR   F     KAK+DYVNEPIP   L+G WELFNEEE E A++   PYGG M+EI 
Sbjct: 361 VLLDRTPLFPKRFFKAKSDYVNEPIPKAALEGTWELFNEEEAEAAEMILSPYGGIMDEIS 420

Query: 421 ESEIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYR 480
           ESE PFPHRAGNLYKI + V W EE ED  ++HINWIRKL S++ P+  +  + + + YR
Sbjct: 421 ESETPFPHRAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSFVGPYVSKNPRAAYINYR 479

BLAST of Moc03g24910 vs. NCBI nr
Match: XP_023542351.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 718.4 bits (1853), Expect = 4.2e-203
Identity = 346/481 (71.93%), Postives = 404/481 (83.99%), Query Frame = 0

Query: 1   MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMN 60
           MKSPIT   YTFVV+SF W ASA N+EDFLHCLSLHSPNSSSISK IYTP D SYS ++N
Sbjct: 1   MKSPITLFVYTFVVLSFAWAASAHNHEDFLHCLSLHSPNSSSISKTIYTPNDSSYSSVLN 60

Query: 61  FSTHNLGLRTAKSFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYD 120
           FS HNL   T ++ KPQ IVTPLQV+ I+ SIICAK  GFQIRTRSGGHDYE LS +S  
Sbjct: 61  FSIHNLRFATPRTPKPQVIVTPLQVTEIKASIICAKTTGFQIRTRSGGHDYEGLSYVS-- 120

Query: 121 DIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGG 180
           D+PF+VVD+IN+RSI+V+ + NVAWVQSGATIGELYYRIA+KSKTLGFPAG+CPTVGVGG
Sbjct: 121 DVPFIVVDLINLRSINVDAKNNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGG 180

Query: 181 HFSGGGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVA 240
           HFSGGGYG+MLRKFGLA DNVIDAYF+DVNG LH+RKSMGEDVFWA+RGGGGASF I+VA
Sbjct: 181 HFSGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVA 240

Query: 241 WKIKLVQVPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNG 300
           WKIKL+QVPP+VTVF +TRNLEQNATNL+HRWQY+A+KQDD+L I+I +RG+NS N Q+G
Sbjct: 241 WKIKLLQVPPIVTVFTVTRNLEQNATNLIHRWQYIASKQDDRLFIRIIIRGVNSTNPQHG 300

Query: 301 -KRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLD 360
            +RT E +F+S+FLG++EELLPIMQNTFPELGLTKEDCIEMSWIES+L+FAGF  GQPL+
Sbjct: 301 SQRTLEAAFNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLN 360

Query: 361 VLLDRNVAFEAN-GKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIP 420
           VLLDR   F     KAK+DYVNEPIP   L+G WELF EEE E A++   PYGG M+EI 
Sbjct: 361 VLLDRTPLFPKRFFKAKSDYVNEPIPKAALEGTWELFKEEEAEAAEMILSPYGGIMDEIS 420

Query: 421 ESEIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYR 480
           ESE PFPHRAGNLYKI + V W EE ED  ++HINWIRKL SY+ P+  +  + + + YR
Sbjct: 421 ESETPFPHRAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSYVGPYVSKNPRAAYINYR 479

BLAST of Moc03g24910 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 3.1e-124
Identity = 219/467 (46.90%), Postives = 314/467 (67.24%), Query Frame = 0

Query: 15  ISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTP--TDPSYSLIMNFSTHNLGLRTAK 74
           +S++ ++ ++  +DF+ CL  +S  S  +    +T      S+ L +  S  NL      
Sbjct: 21  LSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSAQNLRYLMPS 80

Query: 75  SFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYDDIPFVVVDMINI 134
           + KP+ I  PL  +H+Q +++CAKK    +R RSGGHDYE LS +S  +  FV+VD+  +
Sbjct: 81  NPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFVIVDLSKL 140

Query: 135 RSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYGMMLR 194
           R I V++E N AWV +GA+IGE+YYRI +KSK  GFPAGLC ++G+GGH  GG YG M+R
Sbjct: 141 RQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAYGSMMR 200

Query: 195 KFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQVPPVV 254
           KFGL ADNV+DA  VD +GK+ NR +MGEDVFWAIRGGGG SF +I+AWKIKLV VP +V
Sbjct: 201 KFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPEIV 260

Query: 255 TVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSFDSLF 314
           TVF +TR LEQ+ T L+++WQ VA+K D+ L I++ ++   S   ++ +RT  TS+   F
Sbjct: 261 TVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQ-PTSKTPKSKERTISTSYQGQF 320

Query: 315 LGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAFEANG 374
           LG A  LL +MQ +FP+LGLTK+DC+E SWI+SV++ AGF    P + LLD    F+   
Sbjct: 321 LGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDGKSLFKNYF 380

Query: 375 KAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRAGNLY 434
           KAK+DYV EPIP+ GL+G+WE   EE+       + PYGG M +IPE+E PFPHR+G L+
Sbjct: 381 KAKSDYVEEPIPVEGLEGLWEKLLEEDSPLT--IWNPYGGMMAKIPETETPFPHRSGTLF 440

Query: 435 KIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYRN 480
           KI +  +W ++ + +  KH+ W+R++ SYM  +  +  + + + YR+
Sbjct: 441 KIQWLTLW-QDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRD 483

BLAST of Moc03g24910 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 5.9e-123
Identity = 219/445 (49.21%), Postives = 297/445 (66.74%), Query Frame = 0

Query: 21  ASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMNFSTHNLGLRTAKSFKPQAIV 80
           A+ SN   FL CLSL   +S+ +SK+I+TP D S+S ++  S  N         KP  I+
Sbjct: 29  ANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLIL 88

Query: 81  TPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYDDIPFVVVDMINIRSIDVNVE 140
           TP+Q S +Q ++ CA++ G  IRTRSGGHDYE LS +++   PFV++D+ N+RSI V+V+
Sbjct: 89  TPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHK--PFVILDLRNLRSITVDVD 148

Query: 141 KNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYGMMLRKFGLAADN 200
               WVQ+GATIGELYY I +K++TL FPAG+CPTVGVGGHFSGGGYG +LRK GLAAD+
Sbjct: 149 NRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADH 208

Query: 201 VIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQVPPVVTVFEITRN 260
           VIDA  VD  G++  R+ MGED FWAIRGGGG+SF ++++WKI L+ VP  VTVF +T+ 
Sbjct: 209 VIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKF 268

Query: 261 LEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSFDSLFLGQAEELL 320
            EQ+A  ++HRWQ+VA+K  D L I++ L        Q  K     SF  L+LG  + LL
Sbjct: 269 SEQSALKIIHRWQFVADKVSDDLFIRVML--------QRYKNMVRASFPGLYLGSVKNLL 328

Query: 321 PIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAFEANGKAKTDYVN 380
            ++   FPELGL ++DC EMSWIESV+ FA   G +P++VL  R  A  A  KAK+D+V 
Sbjct: 329 KMVNKEFPELGLEEDDCTEMSWIESVIWFAEL-GEEPINVLTKRTRASLA-FKAKSDFVQ 388

Query: 381 EPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRAGNLYKIHYSVVW 440
           EP+P   +  +W    E E E AQ+ F P+GGKM+EI + E PFPHR GN+Y+I Y   W
Sbjct: 389 EPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYW 448

Query: 441 TEEDEDTTQKHINWIRKLESYMTPF 466
                D  +K++ W+ ++   M+ F
Sbjct: 449 R---GDVKEKYMRWVERVYDDMSEF 458

BLAST of Moc03g24910 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 1.3e-122
Identity = 221/481 (45.95%), Postives = 321/481 (66.74%), Query Frame = 0

Query: 2   KSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLI--- 61
           K   T    T ++IS + ++S++  +DF+ CL  +S  S  I+   ++P D + +L    
Sbjct: 7   KRNATLFLVTLLLIS-VPLSSSTLQQDFVKCLVDNSDVSFPITASFFSP-DQNATLFKEE 66

Query: 62  MNFSTHNLGLRTAKSFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSIS 121
           +  +  NL   T  + KP  I  PL  +H+Q +++CAKK    +R RSGGHDYE LS ++
Sbjct: 67  LESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVA 126

Query: 122 YDDIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGV 181
            D+ PFV+VD+  +R +DV+++ N AW  +GATIGE+YYRI +KS+T GFPAGLC ++G+
Sbjct: 127 EDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGI 186

Query: 182 GGHFSGGGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDII 241
           GGH  GG YG M+RKFGL ADNV+DA  VD NG++ +R +MGEDVFWAIRGGGG SF +I
Sbjct: 187 GGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVI 246

Query: 242 VAWKIKLVQVPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQ 301
           +AWKIKLV VP  VTVF +T+ LEQ+ T ++++W+ +A+K DD L I++ +    S   +
Sbjct: 247 LAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVII-SPASKTTK 306

Query: 302 NGKRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPL 361
            G RT   S+ + FLG +  LL +MQ +FPELGLTK+DC EMSWI+SV++ AGF      
Sbjct: 307 PGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAP 366

Query: 362 DVLLDRNVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIP 421
           + LL     F+ + KAK+D+V EPIP+ GL+G+WE F EE+       + PYGG M+ I 
Sbjct: 367 EALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYGGMMSRIS 426

Query: 422 ESEIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYR 480
           ESEIPFPHR G L+KI +   W ++ + + ++H+ WIR++ SYM  +  +  + + + YR
Sbjct: 427 ESEIPFPHRNGTLFKIQWLSTW-QDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYR 481

BLAST of Moc03g24910 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 4.6e-120
Identity = 214/454 (47.14%), Postives = 293/454 (64.54%), Query Frame = 0

Query: 15  ISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMNFSTHNLGLRTAKSF 74
           IS L  A  SN+EDFL CLS    +++   K+I+T  D S+  I++ S  N     +++ 
Sbjct: 17  ISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETP 76

Query: 75  KPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYDDIPFVVVDMINIRS 134
           KP +I+TP++ S +Q  I CA+ HG  +RTRS GH YE LS I+Y+  PF V+D+ N+RS
Sbjct: 77  KPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNK-PFAVIDLRNLRS 136

Query: 135 IDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYGMMLRKF 194
           I ++V+    WVQ+GAT GELYY I + +K+L FPAG+ PTVGVGG FSGGGYG +LRK+
Sbjct: 137 ISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKY 196

Query: 195 GLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQVPPVVTV 254
           GLAADN+IDA  VD +G++ +R++MGED FWAIRGGGG+SF +I++WK+KLV VP  +TV
Sbjct: 197 GLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITV 256

Query: 255 FEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSFDSLFLG 314
           F++ +  ++ A  ++ +WQY A+K  D L I+ TL   N N            F  L++G
Sbjct: 257 FKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSNKN-------AVHALFTGLYIG 316

Query: 315 QAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAFEANGKA 374
               LL +M+  FPELGL KE C EMSWIESVL FA F  G+ L VL +R      + K 
Sbjct: 317 PVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLTNRE-RTSLSFKG 376

Query: 375 KTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRAGNLYKI 434
           K D+V EPIP   +Q IW      E    +I   P+GGKM+E+ E E PFPHR GNLY+I
Sbjct: 377 KDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEI 436

Query: 435 HYSVVWTEEDEDT---TQKHINWIRKLESYMTPF 466
            Y   W EE++     T K++ W+  +  +MTP+
Sbjct: 437 QYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPY 461

BLAST of Moc03g24910 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 4.6e-120
Identity = 221/475 (46.53%), Postives = 301/475 (63.37%), Query Frame = 0

Query: 7   SLAYTFVVISFLWVASAS--NYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMNFSTH 66
           +L      + F+W +S+S  N E F  CL+ +S     IS  I+   + SYS ++  +  
Sbjct: 4   ALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIR 63

Query: 67  NLGLRTAKSFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYDDIPF 126
           NL   T  + KP  I+     SH+Q +I C K+H  Q++ RSGGHDY+ LS ++Y   PF
Sbjct: 64  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 123

Query: 127 VVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSG 186
            V+DM N+RS+DV+V    AWVQ+GA +GE+YY I +KSKTL +PAG+CPTVGVGGH SG
Sbjct: 124 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 183

Query: 187 GGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIK 246
           GGYG M+RK+GL  DN IDA  VDVNGK+ +RK MGED++WAI GGGG S+ +++A+KI 
Sbjct: 184 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 243

Query: 247 LVQVPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTR 306
           LV+VP  VTVF I+R LEQNAT+++HRWQ VA K  D+L I+  +  +N     + ++T 
Sbjct: 244 LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGT--VSSQKTV 303

Query: 307 ETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDR 366
            T+F ++FLG    LL I+   FPELGL + DC E SWI+SVL +   + G    +LL R
Sbjct: 304 RTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQR 363

Query: 367 NVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPF 426
           N       K K+DYV EPI   GL+ IW+     E+E   + F PYGG+M  I  +  PF
Sbjct: 364 NQPVNYL-KRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMGRISSTVTPF 423

Query: 427 PHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYRN 480
           P+RAGNL+KI Y   W   DE  T +++   RKL  +MTPF  +  + S   YR+
Sbjct: 424 PYRAGNLWKIQYGANW--RDETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRD 471

BLAST of Moc03g24910 vs. ExPASy TrEMBL
Match: A0A6J1DE84 (berberine bridge enzyme-like 13 OS=Momordica charantia OX=3673 GN=LOC111020245 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 1.5e-270
Identity = 462/479 (96.45%), Postives = 467/479 (97.49%), Query Frame = 0

Query: 1   MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMN 60
           MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMN
Sbjct: 1   MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMN 60

Query: 61  FSTHNLGLRTAKSFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYD 120
           FSTHNLGLRTAKSFKPQAIVTPLQVSHIQ SIICAKKHGFQIRTRSGGHDYERLSSISYD
Sbjct: 61  FSTHNLGLRTAKSFKPQAIVTPLQVSHIQXSIICAKKHGFQIRTRSGGHDYERLSSISYD 120

Query: 121 DIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGG 180
           DIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGG
Sbjct: 121 DIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGG 180

Query: 181 HFSGGGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVA 240
           HFSGGGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKS GEDVFWAIRGGGGASF IIVA
Sbjct: 181 HFSGGGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSXGEDVFWAIRGGGGASFXIIVA 240

Query: 241 WKIKLVQVPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNG 300
           WKIKLVQ PPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNG
Sbjct: 241 WKIKLVQXPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNG 300

Query: 301 KRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDV 360
           KRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQ LDV
Sbjct: 301 KRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQXLDV 360

Query: 361 LLDRNVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPES 420
           LLDRNVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPES
Sbjct: 361 LLDRNVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPES 420

Query: 421 EIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYRN 480
           EIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPF  +  + + + YR+
Sbjct: 421 EIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRD 479

BLAST of Moc03g24910 vs. ExPASy TrEMBL
Match: A0A6J1DET9 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020159 PE=3 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 4.5e-211
Identity = 366/480 (76.25%), Postives = 420/480 (87.50%), Query Frame = 0

Query: 1   MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMN 60
           MKSPITSLAY FVV+SF+WVASASNYEDFL CLS HSPN+SSISKI+YTP D SY  I+N
Sbjct: 1   MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNSSISKIVYTPIDISYFSILN 60

Query: 61  FSTHNLGLRTAKSFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYD 120
           FS  NL   TAK+ KPQ IVTPLQVSHIQ SIICAK +GFQIRTRSGGHDYE LS +S D
Sbjct: 61  FSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVS-D 120

Query: 121 DIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGG 180
           ++PFVV+D+ N+RSIDV+VEKNVAWVQSGATIGELYYRIA+KSKTLGFPAGLC TVGVGG
Sbjct: 121 NVPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGG 180

Query: 181 HFSGGGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVA 240
           HFSGGGYG+MLRKFGLAADNVIDAYF+DVNG L +RKSMGEDVFWAIRGGGGASF II+A
Sbjct: 181 HFSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILA 240

Query: 241 WKIKLVQVPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGM-NSNNHQN 300
           WKIKL+ VPPVVTVF ITRNLEQNATNL+H+WQYVA+KQDD+LLI++ L GM NS+N+QN
Sbjct: 241 WKIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQN 300

Query: 301 GKRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLD 360
            K+T E SF+SLFLGQ +ELLPIMQ TFPELGLTKEDCIEMSWIE+VL+FAGF+ GQPL+
Sbjct: 301 XKKTLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQ-GQPLN 360

Query: 361 VLLDRNVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPE 420
           VLLDR++A++   K K+DYVNEPIP  G++GIWELFNEEEV+FAQ+  IPYGGKM+EI E
Sbjct: 361 VLLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISE 420

Query: 421 SEIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYRN 480
            EIPFPHR GNLYKIHY+V+   E EDT Q+ I+WIRKL SYMTPF  +  + + + YR+
Sbjct: 421 XEIPFPHRVGNLYKIHYAVL-LGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRD 477

BLAST of Moc03g24910 vs. ExPASy TrEMBL
Match: A0A6J1DDW6 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020161 PE=3 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 3.7e-205
Identity = 354/479 (73.90%), Postives = 406/479 (84.76%), Query Frame = 0

Query: 1   MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMN 60
           MKSPI SLAY FVV+SF  VASA NYEDFLHCLS H PNSSSISKIIYTPT PSYSL++N
Sbjct: 1   MKSPIISLAYIFVVLSFARVASACNYEDFLHCLSFHFPNSSSISKIIYTPTHPSYSLVLN 60

Query: 61  FSTHNLGLRTAKSFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYD 120
           FS  NL    AK+ KPQ IVTPL+ SH+Q SIICAK +G QIRTRSGGHDYE LS +S  
Sbjct: 61  FSIQNLRFLGAKTPKPQIIVTPLEASHVQASIICAKNNGLQIRTRSGGHDYEGLSYVS-- 120

Query: 121 DIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGG 180
           ++PFVVVD+ N+RSIDV+VE+ VAWVQSGATIGELYYRIA+KSKTLGFPAGLCPTVG+GG
Sbjct: 121 NVPFVVVDLTNLRSIDVDVEQKVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGG 180

Query: 181 HFSGGGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVA 240
           HFSGGGYG+MLRKFGLAADNVIDAYF+DVNGKLH+RKSMGEDVFWAIRGGGGASF I++A
Sbjct: 181 HFSGGGYGVMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLA 240

Query: 241 WKIKLVQVPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNG 300
           WKIKL+QVP +VTVF ITRNLEQNATNL++RWQYVANKQDD+L+IKI L G NSNNH+NG
Sbjct: 241 WKIKLLQVPXLVTVFTITRNLEQNATNLIYRWQYVANKQDDRLIIKIILMGTNSNNHRNG 300

Query: 301 KRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDV 360
           KRT E  F+SLFLG+ E+LLPIMQ TFPELGL KEDCIEMSWIESVL+FA F  GQPL+V
Sbjct: 301 KRTIEAFFNSLFLGKVEDLLPIMQKTFPELGLMKEDCIEMSWIESVLYFAEFPRGQPLNV 360

Query: 361 LLDRNVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPES 420
           LLDR   F+ + K K+DYVNEP+P  GL+GIWELFNE+E +  Q+F IPYGGKMNEI ES
Sbjct: 361 LLDRRSTFKGSFKGKSDYVNEPVPKAGLEGIWELFNEKEAKMVQMFLIPYGGKMNEISES 420

Query: 421 EIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYRN 480
           EIPFPHRAGNLY+IHYS+    ++E   Q++  WIRK  SYM PF  +  + + + YR+
Sbjct: 421 EIPFPHRAGNLYQIHYSIYCGGDEE---QRNKIWIRKFYSYMAPFVSKNPRAAYINYRD 474

BLAST of Moc03g24910 vs. ExPASy TrEMBL
Match: A0A6J1GTC1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456799 PE=3 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 7.0e-204
Identity = 347/481 (72.14%), Postives = 405/481 (84.20%), Query Frame = 0

Query: 1   MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMN 60
           MK PIT   YTFVV+SF W ASA N+EDFLHCLSLHSPNSSSISKIIYTP D SYS ++N
Sbjct: 1   MKPPITLFVYTFVVLSFAWAASAHNHEDFLHCLSLHSPNSSSISKIIYTPNDSSYSSVLN 60

Query: 61  FSTHNLGLRTAKSFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYD 120
           FS HNL   T ++ KPQ IVTPLQV+ I+ SIICAK  GFQIRTRSGGHDYE LS +S  
Sbjct: 61  FSIHNLRFATPQTPKPQVIVTPLQVTEIKASIICAKTTGFQIRTRSGGHDYEGLSYVS-- 120

Query: 121 DIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGG 180
           D+PF+VVD+IN+RSI+V+ E NVAWVQSGATIGELYYRIA+KSKTLGFPAG+CPTVGVGG
Sbjct: 121 DVPFIVVDLINLRSINVDAENNVAWVQSGATIGELYYRIAKKSKTLGFPAGVCPTVGVGG 180

Query: 181 HFSGGGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVA 240
           HFSGGGYG+MLRKFGLA DNVIDAYF+DVNG LH+RKSMGEDVFWA+RGGGGASF I+VA
Sbjct: 181 HFSGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVA 240

Query: 241 WKIKLVQVPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNG 300
           WKIKL+QVPP+VTVF +TRNLEQNATNL+HRWQY+A+KQDD+L I+I +RG+NS N Q+G
Sbjct: 241 WKIKLLQVPPIVTVFTVTRNLEQNATNLIHRWQYIASKQDDRLFIRIIIRGVNSTNPQHG 300

Query: 301 -KRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLD 360
            +RT E +F+S+FLG++EELLPIMQNTFPELGLTKEDCIEMSWIES+L+FAGF  GQPL+
Sbjct: 301 SQRTLEAAFNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLN 360

Query: 361 VLLDRNVAFEAN-GKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIP 420
           VLLDR   F     KAK+DYVNEPIP   L+G WELFNEEE E A++   PYGG M+EI 
Sbjct: 361 VLLDRTPLFPKRFFKAKSDYVNEPIPKAALEGTWELFNEEEAEAAEMILSPYGGIMDEIS 420

Query: 421 ESEIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYR 480
           ESE PFPHRAGNLYKI + V W EE ED  ++HINWIRKL S++ P+  +  + + + YR
Sbjct: 421 ESETPFPHRAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSFVGPYVSKNPRAAYINYR 479

BLAST of Moc03g24910 vs. ExPASy TrEMBL
Match: A0A6J1K043 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490403 PE=3 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 3.9e-202
Identity = 344/481 (71.52%), Postives = 400/481 (83.16%), Query Frame = 0

Query: 1   MKSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMN 60
           MKSPIT   YTFVV+SF W ASA N+EDFLHCLSLHSPNSSSISK IYTP D SYS ++N
Sbjct: 1   MKSPITLFVYTFVVLSFAWAASAHNHEDFLHCLSLHSPNSSSISKTIYTPNDSSYSSVLN 60

Query: 61  FSTHNLGLRTAKSFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYD 120
           FS HNL   T ++ KPQ IVTPLQVS I+ SIICAK  GFQIRTRSGGHDYE LS +S  
Sbjct: 61  FSIHNLRFATPRTPKPQVIVTPLQVSEIKASIICAKTTGFQIRTRSGGHDYEGLSYVS-- 120

Query: 121 DIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGG 180
           D+PF++VD+IN+RSI+V+ + NVAWVQSGATIGELYYRIA+KSKTLGFPAG+CPTVGVGG
Sbjct: 121 DVPFIIVDLINLRSINVDAKNNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGG 180

Query: 181 HFSGGGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVA 240
           HFSGGGYG+MLRKFGLA DNVIDAYF+DVNG LH+RKSMGEDVFWA+RGGGGASF I+VA
Sbjct: 181 HFSGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVA 240

Query: 241 WKIKLVQVPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNG 300
           WKIKL+QVPP+VTVF + R LEQNATNL+HRWQY+A+KQDD+L I+I +RG+NS N Q G
Sbjct: 241 WKIKLLQVPPIVTVFTVPRTLEQNATNLIHRWQYIASKQDDRLFIRIIIRGVNSTNPQQG 300

Query: 301 -KRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLD 360
            +RT E +F+S+FLG++EELLPIMQNTFPELGLTKEDCIEMSWIES+L+FAGF  GQPL+
Sbjct: 301 SQRTLEAAFNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLN 360

Query: 361 VLLDRNVAFEAN-GKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIP 420
           VLLDR   F     KAK+DYVNEPIP   L+G WELF EEE E A++   PYGG M+EI 
Sbjct: 361 VLLDRTPLFPKRFFKAKSDYVNEPIPKAALEGTWELFKEEEAEAAEMILSPYGGIMDEIS 420

Query: 421 ESEIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYR 480
           ESE PFPHRAGNLYKI + V W EE ED  ++HINWIRKL SY+ P+     + + + YR
Sbjct: 421 ESETPFPHRAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSYVAPYVSNNPRAAYINYR 479

BLAST of Moc03g24910 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 446.8 bits (1148), Expect = 2.2e-125
Identity = 219/467 (46.90%), Postives = 314/467 (67.24%), Query Frame = 0

Query: 15  ISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTP--TDPSYSLIMNFSTHNLGLRTAK 74
           +S++ ++ ++  +DF+ CL  +S  S  +    +T      S+ L +  S  NL      
Sbjct: 21  LSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSAQNLRYLMPS 80

Query: 75  SFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYDDIPFVVVDMINI 134
           + KP+ I  PL  +H+Q +++CAKK    +R RSGGHDYE LS +S  +  FV+VD+  +
Sbjct: 81  NPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFVIVDLSKL 140

Query: 135 RSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYGMMLR 194
           R I V++E N AWV +GA+IGE+YYRI +KSK  GFPAGLC ++G+GGH  GG YG M+R
Sbjct: 141 RQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAYGSMMR 200

Query: 195 KFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQVPPVV 254
           KFGL ADNV+DA  VD +GK+ NR +MGEDVFWAIRGGGG SF +I+AWKIKLV VP +V
Sbjct: 201 KFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPEIV 260

Query: 255 TVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSFDSLF 314
           TVF +TR LEQ+ T L+++WQ VA+K D+ L I++ ++   S   ++ +RT  TS+   F
Sbjct: 261 TVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQ-PTSKTPKSKERTISTSYQGQF 320

Query: 315 LGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAFEANG 374
           LG A  LL +MQ +FP+LGLTK+DC+E SWI+SV++ AGF    P + LLD    F+   
Sbjct: 321 LGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDGKSLFKNYF 380

Query: 375 KAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRAGNLY 434
           KAK+DYV EPIP+ GL+G+WE   EE+       + PYGG M +IPE+E PFPHR+G L+
Sbjct: 381 KAKSDYVEEPIPVEGLEGLWEKLLEEDSPLT--IWNPYGGMMAKIPETETPFPHRSGTLF 440

Query: 435 KIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYRN 480
           KI +  +W ++ + +  KH+ W+R++ SYM  +  +  + + + YR+
Sbjct: 441 KIQWLTLW-QDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRD 483

BLAST of Moc03g24910 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 442.6 bits (1137), Expect = 4.2e-124
Identity = 219/445 (49.21%), Postives = 297/445 (66.74%), Query Frame = 0

Query: 21  ASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMNFSTHNLGLRTAKSFKPQAIV 80
           A+ SN   FL CLSL   +S+ +SK+I+TP D S+S ++  S  N         KP  I+
Sbjct: 29  ANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLIL 88

Query: 81  TPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYDDIPFVVVDMINIRSIDVNVE 140
           TP+Q S +Q ++ CA++ G  IRTRSGGHDYE LS +++   PFV++D+ N+RSI V+V+
Sbjct: 89  TPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHK--PFVILDLRNLRSITVDVD 148

Query: 141 KNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYGMMLRKFGLAADN 200
               WVQ+GATIGELYY I +K++TL FPAG+CPTVGVGGHFSGGGYG +LRK GLAAD+
Sbjct: 149 NRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADH 208

Query: 201 VIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQVPPVVTVFEITRN 260
           VIDA  VD  G++  R+ MGED FWAIRGGGG+SF ++++WKI L+ VP  VTVF +T+ 
Sbjct: 209 VIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKF 268

Query: 261 LEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSFDSLFLGQAEELL 320
            EQ+A  ++HRWQ+VA+K  D L I++ L        Q  K     SF  L+LG  + LL
Sbjct: 269 SEQSALKIIHRWQFVADKVSDDLFIRVML--------QRYKNMVRASFPGLYLGSVKNLL 328

Query: 321 PIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAFEANGKAKTDYVN 380
            ++   FPELGL ++DC EMSWIESV+ FA   G +P++VL  R  A  A  KAK+D+V 
Sbjct: 329 KMVNKEFPELGLEEDDCTEMSWIESVIWFAEL-GEEPINVLTKRTRASLA-FKAKSDFVQ 388

Query: 381 EPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRAGNLYKIHYSVVW 440
           EP+P   +  +W    E E E AQ+ F P+GGKM+EI + E PFPHR GN+Y+I Y   W
Sbjct: 389 EPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYW 448

Query: 441 TEEDEDTTQKHINWIRKLESYMTPF 466
                D  +K++ W+ ++   M+ F
Sbjct: 449 R---GDVKEKYMRWVERVYDDMSEF 458

BLAST of Moc03g24910 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 441.4 bits (1134), Expect = 9.3e-124
Identity = 221/481 (45.95%), Postives = 321/481 (66.74%), Query Frame = 0

Query: 2   KSPITSLAYTFVVISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLI--- 61
           K   T    T ++IS + ++S++  +DF+ CL  +S  S  I+   ++P D + +L    
Sbjct: 7   KRNATLFLVTLLLIS-VPLSSSTLQQDFVKCLVDNSDVSFPITASFFSP-DQNATLFKEE 66

Query: 62  MNFSTHNLGLRTAKSFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSIS 121
           +  +  NL   T  + KP  I  PL  +H+Q +++CAKK    +R RSGGHDYE LS ++
Sbjct: 67  LESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVA 126

Query: 122 YDDIPFVVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGV 181
            D+ PFV+VD+  +R +DV+++ N AW  +GATIGE+YYRI +KS+T GFPAGLC ++G+
Sbjct: 127 EDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGI 186

Query: 182 GGHFSGGGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDII 241
           GGH  GG YG M+RKFGL ADNV+DA  VD NG++ +R +MGEDVFWAIRGGGG SF +I
Sbjct: 187 GGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVI 246

Query: 242 VAWKIKLVQVPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQ 301
           +AWKIKLV VP  VTVF +T+ LEQ+ T ++++W+ +A+K DD L I++ +    S   +
Sbjct: 247 LAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVII-SPASKTTK 306

Query: 302 NGKRTRETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPL 361
            G RT   S+ + FLG +  LL +MQ +FPELGLTK+DC EMSWI+SV++ AGF      
Sbjct: 307 PGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAP 366

Query: 362 DVLLDRNVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIP 421
           + LL     F+ + KAK+D+V EPIP+ GL+G+WE F EE+       + PYGG M+ I 
Sbjct: 367 EALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYGGMMSRIS 426

Query: 422 ESEIPFPHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYR 480
           ESEIPFPHR G L+KI +   W ++ + + ++H+ WIR++ SYM  +  +  + + + YR
Sbjct: 427 ESEIPFPHRNGTLFKIQWLSTW-QDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYR 481

BLAST of Moc03g24910 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 433.0 bits (1112), Expect = 3.3e-121
Identity = 221/475 (46.53%), Postives = 301/475 (63.37%), Query Frame = 0

Query: 7   SLAYTFVVISFLWVASAS--NYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMNFSTH 66
           +L      + F+W +S+S  N E F  CL+ +S     IS  I+   + SYS ++  +  
Sbjct: 4   ALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIR 63

Query: 67  NLGLRTAKSFKPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYDDIPF 126
           NL   T  + KP  I+     SH+Q +I C K+H  Q++ RSGGHDY+ LS ++Y   PF
Sbjct: 64  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 123

Query: 127 VVVDMINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSG 186
            V+DM N+RS+DV+V    AWVQ+GA +GE+YY I +KSKTL +PAG+CPTVGVGGH SG
Sbjct: 124 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 183

Query: 187 GGYGMMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIK 246
           GGYG M+RK+GL  DN IDA  VDVNGK+ +RK MGED++WAI GGGG S+ +++A+KI 
Sbjct: 184 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 243

Query: 247 LVQVPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTR 306
           LV+VP  VTVF I+R LEQNAT+++HRWQ VA K  D+L I+  +  +N     + ++T 
Sbjct: 244 LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGT--VSSQKTV 303

Query: 307 ETSFDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDR 366
            T+F ++FLG    LL I+   FPELGL + DC E SWI+SVL +   + G    +LL R
Sbjct: 304 RTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQR 363

Query: 367 NVAFEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPF 426
           N       K K+DYV EPI   GL+ IW+     E+E   + F PYGG+M  I  +  PF
Sbjct: 364 NQPVNYL-KRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMGRISSTVTPF 423

Query: 427 PHRAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFKGRVYQLSRLGYRN 480
           P+RAGNL+KI Y   W   DE  T +++   RKL  +MTPF  +  + S   YR+
Sbjct: 424 PYRAGNLWKIQYGANW--RDETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRD 471

BLAST of Moc03g24910 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 433.0 bits (1112), Expect = 3.3e-121
Identity = 214/454 (47.14%), Postives = 293/454 (64.54%), Query Frame = 0

Query: 15  ISFLWVASASNYEDFLHCLSLHSPNSSSISKIIYTPTDPSYSLIMNFSTHNLGLRTAKSF 74
           IS L  A  SN+EDFL CLS    +++   K+I+T  D S+  I++ S  N     +++ 
Sbjct: 17  ISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETP 76

Query: 75  KPQAIVTPLQVSHIQLSIICAKKHGFQIRTRSGGHDYERLSSISYDDIPFVVVDMINIRS 134
           KP +I+TP++ S +Q  I CA+ HG  +RTRS GH YE LS I+Y+  PF V+D+ N+RS
Sbjct: 77  KPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNK-PFAVIDLRNLRS 136

Query: 135 IDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYGMMLRKF 194
           I ++V+    WVQ+GAT GELYY I + +K+L FPAG+ PTVGVGG FSGGGYG +LRK+
Sbjct: 137 ISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKY 196

Query: 195 GLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQVPPVVTV 254
           GLAADN+IDA  VD +G++ +R++MGED FWAIRGGGG+SF +I++WK+KLV VP  +TV
Sbjct: 197 GLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITV 256

Query: 255 FEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSFDSLFLG 314
           F++ +  ++ A  ++ +WQY A+K  D L I+ TL   N N            F  L++G
Sbjct: 257 FKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSNKN-------AVHALFTGLYIG 316

Query: 315 QAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAFEANGKA 374
               LL +M+  FPELGL KE C EMSWIESVL FA F  G+ L VL +R      + K 
Sbjct: 317 PVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLTNRE-RTSLSFKG 376

Query: 375 KTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRAGNLYKI 434
           K D+V EPIP   +Q IW      E    +I   P+GGKM+E+ E E PFPHR GNLY+I
Sbjct: 377 KDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEI 436

Query: 435 HYSVVWTEEDEDT---TQKHINWIRKLESYMTPF 466
            Y   W EE++     T K++ W+  +  +MTP+
Sbjct: 437 QYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPY 461

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152545.15.2e-27096.24berberine bridge enzyme-like 13 [Momordica charantia][more]
XP_022152423.19.4e-21176.25berberine bridge enzyme-like 15 [Momordica charantia][more]
XP_022152425.17.7e-20573.90berberine bridge enzyme-like 18 [Momordica charantia][more]
XP_022954564.11.5e-20372.14berberine bridge enzyme-like 18 [Cucurbita moschata][more]
XP_023542351.14.2e-20371.93berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q93ZA33.1e-12446.90Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=... [more]
Q9SVG55.9e-12349.21Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=... [more]
O647431.3e-12245.95Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV... [more]
Q9FI214.6e-12047.14Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=... [more]
Q9SA854.6e-12046.53Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1DE841.5e-27096.45berberine bridge enzyme-like 13 OS=Momordica charantia OX=3673 GN=LOC111020245 P... [more]
A0A6J1DET94.5e-21176.25berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020159 P... [more]
A0A6J1DDW63.7e-20573.90berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020161 P... [more]
A0A6J1GTC17.0e-20472.14berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456799 PE... [more]
A0A6J1K0433.9e-20271.52berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490403 PE=3... [more]
Match NameE-valueIdentityDescription
AT1G30760.12.2e-12546.90FAD-binding Berberine family protein [more]
AT4G20820.14.2e-12449.21FAD-binding Berberine family protein [more]
AT2G34790.19.3e-12445.95FAD-binding Berberine family protein [more]
AT1G30700.13.3e-12146.53FAD-binding Berberine family protein [more]
AT5G44440.13.3e-12147.14FAD-binding Berberine family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 137..367
e-value: 4.5E-130
score: 436.4
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 23..132
e-value: 5.9E-35
score: 121.3
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 76..214
e-value: 2.6E-24
score: 85.5
NoneNo IPR availableGENE3D3.40.462.20coord: 251..469
e-value: 4.5E-130
score: 436.4
NoneNo IPR availablePANTHERPTHR32448:SF142CANNABIDIOLIC ACID SYNTHASE-LIKEcoord: 9..479
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 9..479
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 72..248
score: 15.742038
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 19..249

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc03g24910.1Moc03g24910.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0071949 FAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding