Lag0017476 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0017476
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionWAT1-related protein
Locationchr5: 4123821 .. 4125587 (+)
RNA-Seq ExpressionLag0017476
SyntenyLag0017476
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAAAGAAACAATGGAAGATTTTGCAATAATGGGTGGGTTGGTGGGAGTGCAGTTTGTTTATGCAGGGAACTCTGTTTTCATGAGTTTTCTAATGTCTTTGGGAATTGATCCTTTAACTCTTGTCATCTTCTCAACCTTCTCAACTTTTCTCATTGTCTCTCCCATTGCAGTTTATTTTGAAAGGTTCTTCTTTTCTCTCTTTGTTTAGCCCGTTCGATAACGATTTTGTTTTTAGTTTTCTGTTTTTGAAGATGGGCCCGTTTCTTCACGATTTCTTTACTAGGGTTGAAGAACGTTTGTAAGAACTCGGAGGGGAAAGTAGTGTTTATAAGTTTAATTTTAAAAAACCAGACACTAAAAATCAAATGGTTATGGAAGGGAGCTTTGTTTTTCTCCTTTGTTTTTGGTTGAATGTAAATGATGAAACAGAGAAGTTTGTGGTTTTGCAGGTCTAATTGGCCAAAGAAACTTAGTCTAAAGTTGGTTTCTCAGCTTTTTCTCATATCCTTTGGAGGGTAAGCATTGAAAAGTTTCCATTTCCACAGCATCTTTAGGTTCTATAGCTATGAAAAGTGATTTGGTAGATTGTGTTTGTTTGCAGGGTTACATTGTTTCAGTCCCTTTTCTTGAAAGGAATACAGCTAACTTCGCCGACGTTAGCGACGGCGATGCCGAATCTCGCCCCGGGAATGATCTTTGCCATTGCTTGGATCTTTAGGTACATTGAAGCAAGTTTCTGTTATGGTTAAAATAAGGTTTGTTTAAGAGCTTTTGTTTGTGTTGTTTGTTTAATGCAGATTAGAAAAAGTTCGGCTAAGTTGCATATATAGCAAGATCAAGATTTTAGGAACATTTCTATGTGTGATAGGCGCGTTCACGATGAGCGCAATGCACGGCGCTGCAAAAGATAATATCACGCAGCGATCGCCTGCGACCGAGCATACGTTCAATGAACAGAAGATCCTCGGTTGCTTGTATCTGTTCTCGGCGATCATCGTTTTGTCGAGCATAGTTGTTCTACAGGTATGTACATGTACAAGTGTGTTTTGTTTGCACATATCTTGGTATGAAGAATGCAAAAGGTGAGAGGGAGATTTCGATGTTTTGAATCGTGTGAGAATGGACAGGCATCGACGCTAGGCGATTTCCCGGCACCGATGTCGTTGTGCGCGATAACGTCGCTGATTGGAGTGATCTTAACCATAGTTGTACAGATAGTTCAAAATCAGAAACTGGAAATTGGTTGGCCACTTCTTAGTGTTAAAGAATTGGCTGCCTATTGTATTCTAGTAAGCAGCCTTCAATTCTGTGCTATTTTGAGATCATTCTTTGTACATATTGTGTGCATATTGATGTATGCTTTTACAAATCTTGCTGACGAGAAGGGCGGAACCATTAGCGGGGCTTGCGTGAGCTTCAACGGGTGGGCGATGAAGAAACGAGGGCCGGTTCTCGTGTCGATGTTTAGTCCGATCGGAACCGTTTGCTCACTCATTCTCTCCGTTTTCACCTGGGGAGAATCCATCAACATTGGGAGGTACATTTTCCACCCTTTTTTCGTTTTTCGTCGTCGACGTTTCGTCCTAGTTAAGCAAGACTTTGGAGCATAATGTTAATATTTGGCTCTCATTGCAGCCTTGCTGGTATGTTCCTCATGTTCACCGGGCTCTACTTCGTGCTGTGGGCGAAAGGGAAGGAACGTTACGACAACCGAAACGGCCCCGAGAGTGAGTTCGACGTTGACAAGCCTCTTTTAAGTTGA

mRNA sequence

ATGAAGAAAGAAACAATGGAAGATTTTGCAATAATGGGTGGGTTGGTGGGAGTGCAGTTTGTTTATGCAGGGAACTCTGTTTTCATGAGTTTTCTAATGTCTTTGGGAATTGATCCTTTAACTCTTGTCATCTTCTCAACCTTCTCAACTTTTCTCATTGTCTCTCCCATTGCAGTTTATTTTGAAAGGTCTAATTGGCCAAAGAAACTTAGTCTAAAGTTGGTTTCTCAGCTTTTTCTCATATCCTTTGGAGGGGTTACATTGTTTCAGTCCCTTTTCTTGAAAGGAATACAGCTAACTTCGCCGACGTTAGCGACGGCGATGCCGAATCTCGCCCCGGGAATGATCTTTGCCATTGCTTGGATCTTTAGATTAGAAAAAGTTCGGCTAAGTTGCATATATAGCAAGATCAAGATTTTAGGAACATTTCTATGTGTGATAGGCGCGTTCACGATGAGCGCAATGCACGGCGCTGCAAAAGATAATATCACGCAGCGATCGCCTGCGACCGAGCATACGTTCAATGAACAGAAGATCCTCGGTTGCTTGTATCTGTTCTCGGCGATCATCGTTTTGTCGAGCATAGTTGTTCTACAGGCATCGACGCTAGGCGATTTCCCGGCACCGATGTCGTTGTGCGCGATAACGTCGCTGATTGGAGTGATCTTAACCATAGTTGTACAGATAGTTCAAAATCAGAAACTGGAAATTGGTTGGCCACTTCTTAGTGTTAAAGAATTGGCTGCCTATTGTATTCTAGGCGGAACCATTAGCGGGGCTTGCGTGAGCTTCAACGGGTGGGCGATGAAGAAACGAGGGCCGGTTCTCGTGTCGATGTTTAGTCCGATCGGAACCGTTTGCTCACTCATTCTCTCCGTTTTCACCTGGGGAGAATCCATCAACATTGGGAGCCTTGCTGGTATGTTCCTCATGTTCACCGGGCTCTACTTCGTGCTGTGGGCGAAAGGGAAGGAACGTTACGACAACCGAAACGGCCCCGAGAGTGAGTTCGACGTTGACAAGCCTCTTTTAAGTTGA

Coding sequence (CDS)

ATGAAGAAAGAAACAATGGAAGATTTTGCAATAATGGGTGGGTTGGTGGGAGTGCAGTTTGTTTATGCAGGGAACTCTGTTTTCATGAGTTTTCTAATGTCTTTGGGAATTGATCCTTTAACTCTTGTCATCTTCTCAACCTTCTCAACTTTTCTCATTGTCTCTCCCATTGCAGTTTATTTTGAAAGGTCTAATTGGCCAAAGAAACTTAGTCTAAAGTTGGTTTCTCAGCTTTTTCTCATATCCTTTGGAGGGGTTACATTGTTTCAGTCCCTTTTCTTGAAAGGAATACAGCTAACTTCGCCGACGTTAGCGACGGCGATGCCGAATCTCGCCCCGGGAATGATCTTTGCCATTGCTTGGATCTTTAGATTAGAAAAAGTTCGGCTAAGTTGCATATATAGCAAGATCAAGATTTTAGGAACATTTCTATGTGTGATAGGCGCGTTCACGATGAGCGCAATGCACGGCGCTGCAAAAGATAATATCACGCAGCGATCGCCTGCGACCGAGCATACGTTCAATGAACAGAAGATCCTCGGTTGCTTGTATCTGTTCTCGGCGATCATCGTTTTGTCGAGCATAGTTGTTCTACAGGCATCGACGCTAGGCGATTTCCCGGCACCGATGTCGTTGTGCGCGATAACGTCGCTGATTGGAGTGATCTTAACCATAGTTGTACAGATAGTTCAAAATCAGAAACTGGAAATTGGTTGGCCACTTCTTAGTGTTAAAGAATTGGCTGCCTATTGTATTCTAGGCGGAACCATTAGCGGGGCTTGCGTGAGCTTCAACGGGTGGGCGATGAAGAAACGAGGGCCGGTTCTCGTGTCGATGTTTAGTCCGATCGGAACCGTTTGCTCACTCATTCTCTCCGTTTTCACCTGGGGAGAATCCATCAACATTGGGAGCCTTGCTGGTATGTTCCTCATGTTCACCGGGCTCTACTTCGTGCTGTGGGCGAAAGGGAAGGAACGTTACGACAACCGAAACGGCCCCGAGAGTGAGTTCGACGTTGACAAGCCTCTTTTAAGTTGA

Protein sequence

MKKETMEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVYFERSNWPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIAWIFRLEKVRLSCIYSKIKILGTFLCVIGAFTMSAMHGAAKDNITQRSPATEHTFNEQKILGCLYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKLEIGWPLLSVKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVFTWGESINIGSLAGMFLMFTGLYFVLWAKGKERYDNRNGPESEFDVDKPLLS
Homology
BLAST of Lag0017476 vs. NCBI nr
Match: XP_038906245.1 (WAT1-related protein At5g47470 [Benincasa hispida])

HSP 1 Score: 590.1 bits (1520), Expect = 1.2e-164
Identity = 312/351 (88.89%), Postives = 327/351 (93.16%), Query Frame = 0

Query: 1   MKKETMEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVY 60
           MK+ETME+FAIM GLVGVQFVYAGNSVFMSFLMSLGI+PLTLVIFSTFSTFLIVSPIAVY
Sbjct: 1   MKRETMENFAIMSGLVGVQFVYAGNSVFMSFLMSLGINPLTLVIFSTFSTFLIVSPIAVY 60

Query: 61  FERSNWPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIA 120
           FER +WPKKLSLKL+ QL LISFGGVTLFQSLFLKGIQLTSP LATAMPNL PG+IFAIA
Sbjct: 61  FERHDWPKKLSLKLILQLLLISFGGVTLFQSLFLKGIQLTSPALATAMPNLTPGLIFAIA 120

Query: 121 WIFRLEKVRLSCIYSKIKILGTFLCVIGAFTMSAMH-----GAAKDNITQRSPATEHTFN 180
           WIFRLEKV+LSCIYSKIKILGTFLCVIGAFTMS MH      AAKDNI ++SP +E  FN
Sbjct: 121 WIFRLEKVQLSCIYSKIKILGTFLCVIGAFTMSIMHSASSDAAAKDNIPRQSPPSEQVFN 180

Query: 181 EQKILGCLYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKL 240
           EQKILGC+YLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGV++TIVVQIVQ QKL
Sbjct: 181 EQKILGCVYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVLITIVVQIVQYQKL 240

Query: 241 EIGWPLL-SVKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVF 300
           +IGWPLL S KELAAY +LGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVF
Sbjct: 241 DIGWPLLISGKELAAYFVLGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVF 300

Query: 301 TWGESINIGSLAGMFLMFTGLYFVLWAKGKERYDNRNGPESEFDVDKPLLS 346
           T GESINIGSLAGMFLMFTGLYFVLWAKGKERY NRN PESEFDVDKPLLS
Sbjct: 301 TLGESINIGSLAGMFLMFTGLYFVLWAKGKERYVNRNCPESEFDVDKPLLS 351

BLAST of Lag0017476 vs. NCBI nr
Match: XP_008448840.2 (PREDICTED: WAT1-related protein At5g47470 [Cucumis melo])

HSP 1 Score: 570.1 bits (1468), Expect = 1.3e-158
Identity = 299/351 (85.19%), Postives = 322/351 (91.74%), Query Frame = 0

Query: 1   MKKETMEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVY 60
           MK+ETMEDFAIM GLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSP+AVY
Sbjct: 12  MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 71

Query: 61  FERSNWPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIA 120
           FER +WPKKLSLKL+ QL LISFGGVTLFQSL LKGIQLTSPTLATAMPNLAPG+IFAIA
Sbjct: 72  FERYSWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 131

Query: 121 WIFRLEKVRLSCIYSKIKILGTFLCVIGAFTMSAMHGAA-----KDNITQRSPATEHTFN 180
           WIFRLEKV+LSCIYSKIKILGT LCVIGAFTMS MH A+     KD++ ++SP +EH F+
Sbjct: 132 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSVMHSASSDTAGKDSVPRQSPLSEHMFD 191

Query: 181 EQKILGCLYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKL 240
           EQKILGC YLFSAII+LSS+VVLQASTLGDFPAPMSLCAITSLIGV+LTI VQIVQ  ++
Sbjct: 192 EQKILGCAYLFSAIIILSSVVVLQASTLGDFPAPMSLCAITSLIGVLLTIGVQIVQYHRI 251

Query: 241 EIGWPLL-SVKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVF 300
           +I WPLL SVKELAAY I+GGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSL+LSV 
Sbjct: 252 DIAWPLLISVKELAAYVIVGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVV 311

Query: 301 TWGESINIGSLAGMFLMFTGLYFVLWAKGKERYDNRNGPESEFDVDKPLLS 346
           T GESINIGSLAGMF+MFTGLYFVLWAKGKERY + N  ESEFDVDKPLLS
Sbjct: 312 TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYVSLNHLESEFDVDKPLLS 362

BLAST of Lag0017476 vs. NCBI nr
Match: XP_004147210.1 (WAT1-related protein At5g47470 isoform X1 [Cucumis sativus])

HSP 1 Score: 567.4 bits (1461), Expect = 8.5e-158
Identity = 299/351 (85.19%), Postives = 318/351 (90.60%), Query Frame = 0

Query: 1   MKKETMEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVY 60
           MK+ETMEDFAIM GLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSP+AVY
Sbjct: 1   MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 60

Query: 61  FERSNWPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIA 120
           FER NWPKKLSLKL+ QL LISFGGVTLFQSL LKGIQLTSPTLATAMPNLAPG+IFAIA
Sbjct: 61  FERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 120

Query: 121 WIFRLEKVRLSCIYSKIKILGTFLCVIGAFTMSAMHG-----AAKDNITQRSPATEHTFN 180
           WIFRLEKV+LSCIYSKIKILGT LCVIGAFTMS MH      A K++I Q+SP +E  F+
Sbjct: 121 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFD 180

Query: 181 EQKILGCLYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKL 240
           EQKILGC YLF AII+LS IVVLQA+TLGDFPAPMSLCAITSLIGV+LTI VQI+Q  ++
Sbjct: 181 EQKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRI 240

Query: 241 EIGWPLL-SVKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVF 300
           +I WPLL  VKELAAY ILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSL+LSV 
Sbjct: 241 DIAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVI 300

Query: 301 TWGESINIGSLAGMFLMFTGLYFVLWAKGKERYDNRNGPESEFDVDKPLLS 346
           T GESINIGSLAGMF+MFTGLYFVLWAKGKERY NRN  ESEFDVDKPLLS
Sbjct: 301 TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYVNRNHLESEFDVDKPLLS 351

BLAST of Lag0017476 vs. NCBI nr
Match: KAA0041079.1 (WAT1-related protein [Cucumis melo var. makuwa] >TYK12029.1 WAT1-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 561.2 bits (1445), Expect = 6.1e-156
Identity = 295/346 (85.26%), Postives = 317/346 (91.62%), Query Frame = 0

Query: 6   MEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVYFERSN 65
           MEDFAIM GLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSP+AVYFER +
Sbjct: 1   MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYS 60

Query: 66  WPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIAWIFRL 125
           WPKKLSLKL+ QL LISFGGVTLFQSL LKGIQLTSPTLATAMPNLAPG+IFAIAWIFRL
Sbjct: 61  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 120

Query: 126 EKVRLSCIYSKIKILGTFLCVIGAFTMSAMHGAA-----KDNITQRSPATEHTFNEQKIL 185
           EKV+LSCIYSKIKILGT LCVIGAFTMS MH A+     KD++ ++SP +EH F+EQKIL
Sbjct: 121 EKVQLSCIYSKIKILGTLLCVIGAFTMSVMHSASSDTAGKDSVPRQSPLSEHMFDEQKIL 180

Query: 186 GCLYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKLEIGWP 245
           GC YLFSAII+LSS+VVLQASTLGDFPAPMSLCAITSLIGV+LTI VQIVQ  +++I WP
Sbjct: 181 GCAYLFSAIIILSSVVVLQASTLGDFPAPMSLCAITSLIGVLLTIGVQIVQYHRIDIAWP 240

Query: 246 LL-SVKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVFTWGES 305
           LL SVKELAAY I+GGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSL+LSV T GES
Sbjct: 241 LLISVKELAAYVIVGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVVTLGES 300

Query: 306 INIGSLAGMFLMFTGLYFVLWAKGKERYDNRNGPESEFDVDKPLLS 346
           INIGSLAGMF+MFTGLYFVLWAKGKERY + N  ESEFDVDKPLLS
Sbjct: 301 INIGSLAGMFMMFTGLYFVLWAKGKERYVSLNHLESEFDVDKPLLS 346

BLAST of Lag0017476 vs. NCBI nr
Match: XP_011650397.1 (WAT1-related protein At5g47470 isoform X2 [Cucumis sativus] >KAE8650056.1 hypothetical protein Csa_009979 [Cucumis sativus])

HSP 1 Score: 545.0 bits (1403), Expect = 4.5e-151
Identity = 287/337 (85.16%), Postives = 306/337 (90.80%), Query Frame = 0

Query: 1   MKKETMEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVY 60
           MK+ETMEDFAIM GLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSP+AVY
Sbjct: 1   MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 60

Query: 61  FERSNWPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIA 120
           FER NWPKKLSLKL+ QL LISFGGVTLFQSL LKGIQLTSPTLATAMPNLAPG+IFAIA
Sbjct: 61  FERYNWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 120

Query: 121 WIFRLEKVRLSCIYSKIKILGTFLCVIGAFTMSAMHG-----AAKDNITQRSPATEHTFN 180
           WIFRLEKV+LSCIYSKIKILGT LCVIGAFTMS MH      A K++I Q+SP +E  F+
Sbjct: 121 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFD 180

Query: 181 EQKILGCLYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKL 240
           EQKILGC YLF AII+LS IVVLQA+TLGDFPAPMSLCAITSLIGV+LTI VQI+Q  ++
Sbjct: 181 EQKILGCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRI 240

Query: 241 EIGWPLL-SVKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVF 300
           +I WPLL  VKELAAY ILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSL+LSV 
Sbjct: 241 DIAWPLLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVI 300

Query: 301 TWGESINIGSLAGMFLMFTGLYFVLWAKGKERYDNRN 332
           T GESINIGSLAGMF+MFTGLYFVLWAKGKERY NRN
Sbjct: 301 TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYVNRN 337

BLAST of Lag0017476 vs. ExPASy Swiss-Prot
Match: Q9FGL0 (WAT1-related protein At5g47470 OS=Arabidopsis thaliana OX=3702 GN=At5g47470 PE=3 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 2.5e-112
Identity = 209/346 (60.40%), Postives = 261/346 (75.43%), Query Frame = 0

Query: 1   MKKETMEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVY 60
           MK E +E+  I+GGLV VQFVYAGNS+ MS+LMSLG+ P T+VIFSTF+TF+I+SP A+ 
Sbjct: 20  MKTEMIEEMVIVGGLVMVQFVYAGNSLLMSYLMSLGLGPFTIVIFSTFATFIILSPFAIL 79

Query: 61  FERSNWPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIA 120
           FER  WP +LSL+L+ +L LISF GVTLFQSLFL+GI+LTSP +ATAMPNLAPG+IF IA
Sbjct: 80  FERKQWPNELSLRLIGKLVLISFAGVTLFQSLFLEGIRLTSPAMATAMPNLAPGLIFFIA 139

Query: 121 WIFRLEKVRLSCIYSKIKILGTFLCVIGAFTMSAMHGAAKDNITQRSPATEHTFNEQKIL 180
           WI  LEK+ L C+YSK+KILGT LCV GA  MS MH  +  +  +        F+  K++
Sbjct: 140 WIVGLEKMNLKCVYSKLKILGTLLCVFGALAMSVMHSTSISH-KEEDDTPIFVFDRDKVV 199

Query: 181 GCLYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKLEI-GW 240
           GC+YL  A+ VLS+ VVLQASTL +FPAP+SL AIT+L+GV++T VV ++QN+K ++   
Sbjct: 200 GCIYLLGAVFVLSTNVVLQASTLAEFPAPISLSAITALLGVLITTVVLLLQNRKTKVLAS 259

Query: 241 PLLSVKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVFTWGES 300
            L+S   L  Y +L G +SGACVSFNGWAMKKRGPV VSMFSP  TV S+  +V T GES
Sbjct: 260 SLISFGNLVGYSVLAGAVSGACVSFNGWAMKKRGPVFVSMFSPFATVISVAFAVLTLGES 319

Query: 301 INIGSLAGMFLMFTGLYFVLWAKGKERYDNRNGPESEFDVDKPLLS 346
           +++GS+ GM LMF GLY VLWAKGKE +      ESEFD  KPLLS
Sbjct: 320 VSLGSVGGMVLMFVGLYLVLWAKGKEGFSEIESFESEFDSKKPLLS 364

BLAST of Lag0017476 vs. ExPASy Swiss-Prot
Match: F4JMI7 (WAT1-related protein At4g16620 OS=Arabidopsis thaliana OX=3702 GN=At4g16620 PE=2 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 5.7e-88
Identity = 177/357 (49.58%), Postives = 241/357 (67.51%), Query Frame = 0

Query: 1   MKKETMEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVY 60
           MK+ET+ +  I+GGL G Q +YAGNS  +S L+SLGIDPL +VI  TF++ L+++P+A  
Sbjct: 2   MKRETLIEAGIIGGLAGAQVIYAGNSELLSQLLSLGIDPLLIVILCTFASVLLITPLAFL 61

Query: 61  FERSNWPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIA 120
            ER  WP+ LS KL  +L L++  GVTLFQ LFL+G++ TS ++ATAMPNL P  IF IA
Sbjct: 62  LERKLWPRSLSFKLKIKLVLVALAGVTLFQGLFLEGMKHTSASMATAMPNLCPAFIFVIA 121

Query: 121 WIFRLEKVRLSCIYSKIKILGTFLCVIGAFTMSAMHGAAKDNITQRS---PATEHTFNEQ 180
           W   +EKV+LSC+YS++K+ GT LCV+GA  MS MH       + ++      E   ++ 
Sbjct: 122 WAAGMEKVKLSCMYSRVKMGGTVLCVMGALIMSLMHSTTATLSSVKTIPIVPDEVVVDKD 181

Query: 181 KILGCLYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKLEI 240
           KILGCLYL  AI  LSS +VLQAS L +FPAP+S+ ++ SL+G I T+ +Q      +E+
Sbjct: 182 KILGCLYLLLAICGLSSSIVLQASILAEFPAPISMFSMVSLMGGITTVALQYALKGSMEM 241

Query: 241 G-WPLLSVKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVFTW 300
           G   ++ +  L  Y ILGG +SG  +SFN W +K++GPV+VS+FSPI TV  +++S FT 
Sbjct: 242 GSASVIGLGHLVGYAILGGLVSGGGLSFNAWVIKRKGPVIVSLFSPIATVVCVVVSAFTM 301

Query: 301 GESINIGSLAGMFLMFTGLYFVLWAKGKE---------RYDNRNGPESEFDVDKPLL 345
            ES N+GS AGM LMF GLYFVLWAKGKE         + D  +   +EFD+ KPLL
Sbjct: 302 EESFNLGSFAGMALMFGGLYFVLWAKGKEDCEEIDEMKQDDEESLLRTEFDLQKPLL 358

BLAST of Lag0017476 vs. ExPASy Swiss-Prot
Match: Q8GXB4 (WAT1-related protein At1g09380 OS=Arabidopsis thaliana OX=3702 GN=At1g09380 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.4e-33
Identity = 109/344 (31.69%), Postives = 171/344 (49.71%), Query Frame = 0

Query: 1   MKKETMEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLV----IFSTFSTFLIVSP 60
           M K  M  F  M   V VQ  YAG ++     M  G+ PL LV    IF+T +TF    P
Sbjct: 1   MAKSDMLPFLAM---VLVQIGYAGMNITSKMAMEAGMKPLILVAYRQIFATIATF----P 60

Query: 61  IAVYFERSNWPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMI 120
           +A + ER   P K++L+++ Q+F  S  G T  Q L+  G+Q +SPT+A A+ NL P + 
Sbjct: 61  VAFFLERKTRP-KITLRILVQVFFCSITGATGNQVLYFVGLQNSSPTIACALTNLLPAVT 120

Query: 121 FAIAWIFRLEKVRLSCIYSKIKILGTFLCVIGAFTMSAMHG------------AAKDNIT 180
           F +A IFR E V +     + K++GT +CVIGA  +S  HG            A  +NIT
Sbjct: 121 FLLAAIFRQETVGIKKASGQAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAYAENIT 180

Query: 181 QRSPATEHTFNEQKILGCLYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILT 240
           +   ++ H+      LG   + +A +  ++  ++Q      F AP +   +  L+G I  
Sbjct: 181 KHGSSSGHS---NFFLGPFLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLLMCLMGSIQC 240

Query: 241 IVVQIVQNQKLEIGWPLLSVKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIG 300
             + ++ +  +   W L S     +    G   S        WAM+++GP+ VS+FSP+ 
Sbjct: 241 GAIALISDHTIS-DWSLSSPLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLL 300

Query: 301 TVCSLILSVFTWG---ESINIGSLAGMFLMFTGLYFVLWAKGKE 326
            V   ++++F+W    E +  G+  G  L+  GLY VLW K +E
Sbjct: 301 LV---VVAIFSWALLEEKLYTGTFMGSALVVIGLYGVLWGKDRE 329

BLAST of Lag0017476 vs. ExPASy Swiss-Prot
Match: Q9M0B8 (WAT1-related protein At4g30420 OS=Arabidopsis thaliana OX=3702 GN=At4g30420 PE=3 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 5.2e-33
Identity = 102/326 (31.29%), Postives = 160/326 (49.08%), Query Frame = 0

Query: 18  VQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVYFERSN--WPKKLSLKLV 77
           +Q  YAG ++F    +  G+ P   +++      + + P      R +      L LK  
Sbjct: 6   IQLCYAGVTLFARATLVHGLSPRVFILYRQAFATIFIFPFLYLSRRKSKIAISSLDLKSF 65

Query: 78  SQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIAWIFRLEKVRLSCIYS 137
           S +FL+S  G+T+ Q+L+L+G+ LTS ++ +A+ N+ P + F I+++   EK+ L  I  
Sbjct: 66  SLIFLVSLIGITINQNLYLEGLYLTSSSMGSAVGNIIPAITFLISFLAGYEKLNLRDIRG 125

Query: 138 KIKILGTFLCVIGAFTMSAMHGAAKDNITQRSPATEHTFNEQK-----ILGCLYLFSAII 197
             KI GT LCV GA +M+ + G    N     P  +      K     ++GCL+LFS+ +
Sbjct: 126 LAKIAGTILCVAGAISMTLLRGPKILNSESALPIAKSVLGHLKDQNTWLIGCLFLFSSTL 185

Query: 198 VLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKLEIGWPLLSVKELAAY 257
             S  ++LQ      +P  +SL A   L G I   VV     +K    W L S  E A  
Sbjct: 186 CWSFWLILQVPISAYYPDNLSLSAWMCLFGTIQCAVVTFFL-EKDPNAWILHSYSEFATC 245

Query: 258 CILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVFTWGESINIGSLAGMFL 317
              G   S    +   WA+ KRGPV  ++F+P+ TV   IL+   + E I  GSL G   
Sbjct: 246 LYAGIGASALSFTVQAWAIAKRGPVFSALFNPLCTVIVTILAALFFHEEIYTGSLIGGLG 305

Query: 318 MFTGLYFVLWAKGKERYDNRNGPESE 337
           +  GLY VLW K K+   N++  +++
Sbjct: 306 VILGLYTVLWGKAKDVMMNQDQRDND 330

BLAST of Lag0017476 vs. ExPASy Swiss-Prot
Match: O80638 (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.2e-32
Identity = 112/339 (33.04%), Postives = 167/339 (49.26%), Query Frame = 0

Query: 15  LVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVYFERSNWPKKLSLKL 74
           +V +QF YAG S+   F ++ G+ P  L  +      + ++P A + +R   P K++L +
Sbjct: 12  VVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRP-KMTLSI 71

Query: 75  VSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIAWIFRLEKVRLSCIY 134
             ++ L+     T+ Q+L+  G++ TS T   AM N+ P   F +AWIFRLEKV +  I+
Sbjct: 72  FFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNVKKIH 131

Query: 135 SKIKILGTFLCVIGAFTMSAMHG-------AAKDNITQRSPATEHTFNEQKILGCLYLFS 194
           S+ KILGT + V GA  M+ + G       A   +I Q S  T     +    G   +  
Sbjct: 132 SQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQDSSNT--GVKQDLTKGASLIAI 191

Query: 195 AIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKLEIGWPL-LSVKE 254
             I  +  + LQA TL  +P  +SL A    +G I + +V +   +     W + L  K 
Sbjct: 192 GCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHLDSKL 251

Query: 255 LAAYCILGGTI-SGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVFTWGESINIGSL 314
           LAA  + GG I SG      G  MK RGPV V+ F+P+  V   IL      E + +G +
Sbjct: 252 LAA--VYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMFLGRI 311

Query: 315 AGMFLMFTGLYFVLWAKGKERYDNRNGPESEF-DVDKPL 344
            G  ++  GLY VLW K K+       P S F D+DK L
Sbjct: 312 LGAIVIVLGLYSVLWGKSKDE------PSSSFSDMDKEL 339

BLAST of Lag0017476 vs. ExPASy TrEMBL
Match: A0A1S3BKN0 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103490881 PE=3 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 6.4e-159
Identity = 299/351 (85.19%), Postives = 322/351 (91.74%), Query Frame = 0

Query: 1   MKKETMEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVY 60
           MK+ETMEDFAIM GLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSP+AVY
Sbjct: 12  MKRETMEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVY 71

Query: 61  FERSNWPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIA 120
           FER +WPKKLSLKL+ QL LISFGGVTLFQSL LKGIQLTSPTLATAMPNLAPG+IFAIA
Sbjct: 72  FERYSWPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIA 131

Query: 121 WIFRLEKVRLSCIYSKIKILGTFLCVIGAFTMSAMHGAA-----KDNITQRSPATEHTFN 180
           WIFRLEKV+LSCIYSKIKILGT LCVIGAFTMS MH A+     KD++ ++SP +EH F+
Sbjct: 132 WIFRLEKVQLSCIYSKIKILGTLLCVIGAFTMSVMHSASSDTAGKDSVPRQSPLSEHMFD 191

Query: 181 EQKILGCLYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKL 240
           EQKILGC YLFSAII+LSS+VVLQASTLGDFPAPMSLCAITSLIGV+LTI VQIVQ  ++
Sbjct: 192 EQKILGCAYLFSAIIILSSVVVLQASTLGDFPAPMSLCAITSLIGVLLTIGVQIVQYHRI 251

Query: 241 EIGWPLL-SVKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVF 300
           +I WPLL SVKELAAY I+GGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSL+LSV 
Sbjct: 252 DIAWPLLISVKELAAYVIVGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVV 311

Query: 301 TWGESINIGSLAGMFLMFTGLYFVLWAKGKERYDNRNGPESEFDVDKPLLS 346
           T GESINIGSLAGMF+MFTGLYFVLWAKGKERY + N  ESEFDVDKPLLS
Sbjct: 312 TLGESINIGSLAGMFMMFTGLYFVLWAKGKERYVSLNHLESEFDVDKPLLS 362

BLAST of Lag0017476 vs. ExPASy TrEMBL
Match: A0A5D3CP19 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1017G00410 PE=3 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 3.0e-156
Identity = 295/346 (85.26%), Postives = 317/346 (91.62%), Query Frame = 0

Query: 6   MEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVYFERSN 65
           MEDFAIM GLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSP+AVYFER +
Sbjct: 1   MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYS 60

Query: 66  WPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIAWIFRL 125
           WPKKLSLKL+ QL LISFGGVTLFQSL LKGIQLTSPTLATAMPNLAPG+IFAIAWIFRL
Sbjct: 61  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 120

Query: 126 EKVRLSCIYSKIKILGTFLCVIGAFTMSAMHGAA-----KDNITQRSPATEHTFNEQKIL 185
           EKV+LSCIYSKIKILGT LCVIGAFTMS MH A+     KD++ ++SP +EH F+EQKIL
Sbjct: 121 EKVQLSCIYSKIKILGTLLCVIGAFTMSVMHSASSDTAGKDSVPRQSPLSEHMFDEQKIL 180

Query: 186 GCLYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKLEIGWP 245
           GC YLFSAII+LSS+VVLQASTLGDFPAPMSLCAITSLIGV+LTI VQIVQ  +++I WP
Sbjct: 181 GCAYLFSAIIILSSVVVLQASTLGDFPAPMSLCAITSLIGVLLTIGVQIVQYHRIDIAWP 240

Query: 246 LL-SVKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVFTWGES 305
           LL SVKELAAY I+GGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSL+LSV T GES
Sbjct: 241 LLISVKELAAYVIVGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVVTLGES 300

Query: 306 INIGSLAGMFLMFTGLYFVLWAKGKERYDNRNGPESEFDVDKPLLS 346
           INIGSLAGMF+MFTGLYFVLWAKGKERY + N  ESEFDVDKPLLS
Sbjct: 301 INIGSLAGMFMMFTGLYFVLWAKGKERYVSLNHLESEFDVDKPLLS 346

BLAST of Lag0017476 vs. ExPASy TrEMBL
Match: A0A6J1L2K3 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111498520 PE=3 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 3.5e-149
Identity = 275/342 (80.41%), Postives = 302/342 (88.30%), Query Frame = 0

Query: 6   MEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVYFERSN 65
           MEDF IM GLV VQFVYAGNSVFMSFLMSL IDPLTL+IF+TF TFLIVSP+A+YFERSN
Sbjct: 1   MEDFGIMSGLVAVQFVYAGNSVFMSFLMSLSIDPLTLIIFTTFFTFLIVSPVALYFERSN 60

Query: 66  WPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIAWIFRL 125
           WP KLSLKL+SQ+ LISFGGVTLFQSLFLKGIQLTSP++ATAMPNLAPG+IF IAWIFRL
Sbjct: 61  WPTKLSLKLISQILLISFGGVTLFQSLFLKGIQLTSPSVATAMPNLAPGLIFVIAWIFRL 120

Query: 126 EKVRLSCIYSKIKILGTFLCVIGAFTMSAMHGAAKDNITQ--RSPATEHTFNEQKILGCL 185
           EKVRLSCIYSKIKILGTFLCVIGAFTMS MH    D      R PA +  F+EQKI+GC+
Sbjct: 121 EKVRLSCIYSKIKILGTFLCVIGAFTMSIMHSTLSDAAANVGRRPA-DQAFDEQKIVGCV 180

Query: 186 YLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKLEIGWPLLS 245
           YL SA++VLSS+VVLQASTLGDFP PMSLCAITSL+GV++TIV+ I   QKLEIGW  LS
Sbjct: 181 YLISAVVVLSSVVVLQASTLGDFPTPMSLCAITSLVGVLITIVIHIALYQKLEIGWSYLS 240

Query: 246 VKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVFTWGESINIG 305
           V++L AYC+L GT+SGACVSFNGWAMKKRGPVLVSMFSPIGTVCSL+LSVFT GE INIG
Sbjct: 241 VEKLIAYCVLSGTVSGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVFTLGEPINIG 300

Query: 306 SLAGMFLMFTGLYFVLWAKGKERYDNRNGPESEFDVDKPLLS 346
           SL GMFLMFTGLYFVLWAK KE Y NRN PESEFD+D+PLLS
Sbjct: 301 SLVGMFLMFTGLYFVLWAKRKEGYANRNRPESEFDIDEPLLS 341

BLAST of Lag0017476 vs. ExPASy TrEMBL
Match: A0A0A0L5H5 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_3G019970 PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 1.0e-148
Identity = 283/332 (85.24%), Postives = 301/332 (90.66%), Query Frame = 0

Query: 6   MEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVYFERSN 65
           MEDFAIM GLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSP+AVYFER N
Sbjct: 1   MEDFAIMSGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPVAVYFERYN 60

Query: 66  WPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIAWIFRL 125
           WPKKLSLKL+ QL LISFGGVTLFQSL LKGIQLTSPTLATAMPNLAPG+IFAIAWIFRL
Sbjct: 61  WPKKLSLKLILQLVLISFGGVTLFQSLLLKGIQLTSPTLATAMPNLAPGLIFAIAWIFRL 120

Query: 126 EKVRLSCIYSKIKILGTFLCVIGAFTMSAMHG-----AAKDNITQRSPATEHTFNEQKIL 185
           EKV+LSCIYSKIKILGT LCVIGAFTMS MH      A K++I Q+SP +E  F+EQKIL
Sbjct: 121 EKVQLSCIYSKIKILGTLLCVIGAFTMSIMHSAPSDVAGKNSIPQQSPLSEQLFDEQKIL 180

Query: 186 GCLYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKLEIGWP 245
           GC YLF AII+LS IVVLQA+TLGDFPAPMSLCAITSLIGV+LTI VQI+Q  +++I WP
Sbjct: 181 GCAYLFVAIIILSCIVVLQAATLGDFPAPMSLCAITSLIGVLLTIGVQIIQYHRIDIAWP 240

Query: 246 LL-SVKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVFTWGES 305
           LL  VKELAAY ILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSL+LSV T GES
Sbjct: 241 LLIGVKELAAYVILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLVLSVITLGES 300

Query: 306 INIGSLAGMFLMFTGLYFVLWAKGKERYDNRN 332
           INIGSLAGMF+MFTGLYFVLWAKGKERY NRN
Sbjct: 301 INIGSLAGMFMMFTGLYFVLWAKGKERYVNRN 332

BLAST of Lag0017476 vs. ExPASy TrEMBL
Match: A0A6J1DJ66 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111021397 PE=3 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 8.6e-148
Identity = 276/343 (80.47%), Postives = 304/343 (88.63%), Query Frame = 0

Query: 6   MEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVYFERSN 65
           MED AIMGGLVGVQFVYAGNSV MSF MS+GIDPLTLVIFSTFSTFLIVSPIAVYFERS 
Sbjct: 1   MEDLAIMGGLVGVQFVYAGNSVLMSFFMSMGIDPLTLVIFSTFSTFLIVSPIAVYFERSK 60

Query: 66  WPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIAWIFRL 125
           WP KL+LKL+SQL LISFGGVTLFQSLFLKGIQLTSP+LATAMPNLAPG+IF IAWIFRL
Sbjct: 61  WPNKLTLKLISQLLLISFGGVTLFQSLFLKGIQLTSPSLATAMPNLAPGLIFVIAWIFRL 120

Query: 126 EKVRLSCIYSKIKILGTFLCVIGAFTMSAMHGAAK--DNITQRSPATEHTFNEQKILGCL 185
           EKV LSCIYSKIKILGT LCV+GAFTM+ MH A+K    I       +   + QKILGCL
Sbjct: 121 EKVELSCIYSKIKILGTLLCVVGAFTMTIMHSASKVDSTILPLFDEQDQILDRQKILGCL 180

Query: 186 YLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKLEIGWP-LL 245
           YLF+A++VLSSIVVLQA+TLGDFPAPMSLCAITSLIGV+ TI VQIV+ + +EI WP  +
Sbjct: 181 YLFTAVVVLSSIVVLQAATLGDFPAPMSLCAITSLIGVMFTIGVQIVEYKTVEIVWPWFV 240

Query: 246 SVKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVFTWGESINI 305
           SVKE+AAYC++GG++SGACVSFNGWAMKKRGPVLVSMFSPIGTV SLILS+FT GESIN+
Sbjct: 241 SVKEMAAYCVIGGSVSGACVSFNGWAMKKRGPVLVSMFSPIGTVSSLILSLFTLGESINV 300

Query: 306 GSLAGMFLMFTGLYFVLWAKGKERYDNRNGPESEFDVDKPLLS 346
           GSLAGMFLMFTGLYFVLWAK KE Y  RNGPESEFD+DKPLL+
Sbjct: 301 GSLAGMFLMFTGLYFVLWAKRKESYGTRNGPESEFDIDKPLLT 343

BLAST of Lag0017476 vs. TAIR 10
Match: AT5G47470.1 (Nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 406.8 bits (1044), Expect = 1.8e-113
Identity = 209/346 (60.40%), Postives = 261/346 (75.43%), Query Frame = 0

Query: 1   MKKETMEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVY 60
           MK E +E+  I+GGLV VQFVYAGNS+ MS+LMSLG+ P T+VIFSTF+TF+I+SP A+ 
Sbjct: 20  MKTEMIEEMVIVGGLVMVQFVYAGNSLLMSYLMSLGLGPFTIVIFSTFATFIILSPFAIL 79

Query: 61  FERSNWPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIA 120
           FER  WP +LSL+L+ +L LISF GVTLFQSLFL+GI+LTSP +ATAMPNLAPG+IF IA
Sbjct: 80  FERKQWPNELSLRLIGKLVLISFAGVTLFQSLFLEGIRLTSPAMATAMPNLAPGLIFFIA 139

Query: 121 WIFRLEKVRLSCIYSKIKILGTFLCVIGAFTMSAMHGAAKDNITQRSPATEHTFNEQKIL 180
           WI  LEK+ L C+YSK+KILGT LCV GA  MS MH  +  +  +        F+  K++
Sbjct: 140 WIVGLEKMNLKCVYSKLKILGTLLCVFGALAMSVMHSTSISH-KEEDDTPIFVFDRDKVV 199

Query: 181 GCLYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKLEI-GW 240
           GC+YL  A+ VLS+ VVLQASTL +FPAP+SL AIT+L+GV++T VV ++QN+K ++   
Sbjct: 200 GCIYLLGAVFVLSTNVVLQASTLAEFPAPISLSAITALLGVLITTVVLLLQNRKTKVLAS 259

Query: 241 PLLSVKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVFTWGES 300
            L+S   L  Y +L G +SGACVSFNGWAMKKRGPV VSMFSP  TV S+  +V T GES
Sbjct: 260 SLISFGNLVGYSVLAGAVSGACVSFNGWAMKKRGPVFVSMFSPFATVISVAFAVLTLGES 319

Query: 301 INIGSLAGMFLMFTGLYFVLWAKGKERYDNRNGPESEFDVDKPLLS 346
           +++GS+ GM LMF GLY VLWAKGKE +      ESEFD  KPLLS
Sbjct: 320 VSLGSVGGMVLMFVGLYLVLWAKGKEGFSEIESFESEFDSKKPLLS 364

BLAST of Lag0017476 vs. TAIR 10
Match: AT4G16620.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 325.9 bits (834), Expect = 4.0e-89
Identity = 177/357 (49.58%), Postives = 241/357 (67.51%), Query Frame = 0

Query: 1   MKKETMEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVY 60
           MK+ET+ +  I+GGL G Q +YAGNS  +S L+SLGIDPL +VI  TF++ L+++P+A  
Sbjct: 2   MKRETLIEAGIIGGLAGAQVIYAGNSELLSQLLSLGIDPLLIVILCTFASVLLITPLAFL 61

Query: 61  FERSNWPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIA 120
            ER  WP+ LS KL  +L L++  GVTLFQ LFL+G++ TS ++ATAMPNL P  IF IA
Sbjct: 62  LERKLWPRSLSFKLKIKLVLVALAGVTLFQGLFLEGMKHTSASMATAMPNLCPAFIFVIA 121

Query: 121 WIFRLEKVRLSCIYSKIKILGTFLCVIGAFTMSAMHGAAKDNITQRS---PATEHTFNEQ 180
           W   +EKV+LSC+YS++K+ GT LCV+GA  MS MH       + ++      E   ++ 
Sbjct: 122 WAAGMEKVKLSCMYSRVKMGGTVLCVMGALIMSLMHSTTATLSSVKTIPIVPDEVVVDKD 181

Query: 181 KILGCLYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKLEI 240
           KILGCLYL  AI  LSS +VLQAS L +FPAP+S+ ++ SL+G I T+ +Q      +E+
Sbjct: 182 KILGCLYLLLAICGLSSSIVLQASILAEFPAPISMFSMVSLMGGITTVALQYALKGSMEM 241

Query: 241 G-WPLLSVKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVFTW 300
           G   ++ +  L  Y ILGG +SG  +SFN W +K++GPV+VS+FSPI TV  +++S FT 
Sbjct: 242 GSASVIGLGHLVGYAILGGLVSGGGLSFNAWVIKRKGPVIVSLFSPIATVVCVVVSAFTM 301

Query: 301 GESINIGSLAGMFLMFTGLYFVLWAKGKE---------RYDNRNGPESEFDVDKPLL 345
            ES N+GS AGM LMF GLYFVLWAKGKE         + D  +   +EFD+ KPLL
Sbjct: 302 EESFNLGSFAGMALMFGGLYFVLWAKGKEDCEEIDEMKQDDEESLLRTEFDLQKPLL 358

BLAST of Lag0017476 vs. TAIR 10
Match: AT1G09380.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 145.2 bits (365), Expect = 9.7e-35
Identity = 109/344 (31.69%), Postives = 171/344 (49.71%), Query Frame = 0

Query: 1   MKKETMEDFAIMGGLVGVQFVYAGNSVFMSFLMSLGIDPLTLV----IFSTFSTFLIVSP 60
           M K  M  F  M   V VQ  YAG ++     M  G+ PL LV    IF+T +TF    P
Sbjct: 1   MAKSDMLPFLAM---VLVQIGYAGMNITSKMAMEAGMKPLILVAYRQIFATIATF----P 60

Query: 61  IAVYFERSNWPKKLSLKLVSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMI 120
           +A + ER   P K++L+++ Q+F  S  G T  Q L+  G+Q +SPT+A A+ NL P + 
Sbjct: 61  VAFFLERKTRP-KITLRILVQVFFCSITGATGNQVLYFVGLQNSSPTIACALTNLLPAVT 120

Query: 121 FAIAWIFRLEKVRLSCIYSKIKILGTFLCVIGAFTMSAMHG------------AAKDNIT 180
           F +A IFR E V +     + K++GT +CVIGA  +S  HG            A  +NIT
Sbjct: 121 FLLAAIFRQETVGIKKASGQAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAYAENIT 180

Query: 181 QRSPATEHTFNEQKILGCLYLFSAIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILT 240
           +   ++ H+      LG   + +A +  ++  ++Q      F AP +   +  L+G I  
Sbjct: 181 KHGSSSGHS---NFFLGPFLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLLMCLMGSIQC 240

Query: 241 IVVQIVQNQKLEIGWPLLSVKELAAYCILGGTISGACVSFNGWAMKKRGPVLVSMFSPIG 300
             + ++ +  +   W L S     +    G   S        WAM+++GP+ VS+FSP+ 
Sbjct: 241 GAIALISDHTIS-DWSLSSPLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLL 300

Query: 301 TVCSLILSVFTWG---ESINIGSLAGMFLMFTGLYFVLWAKGKE 326
            V   ++++F+W    E +  G+  G  L+  GLY VLW K +E
Sbjct: 301 LV---VVAIFSWALLEEKLYTGTFMGSALVVIGLYGVLWGKDRE 329

BLAST of Lag0017476 vs. TAIR 10
Match: AT4G30420.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 143.3 bits (360), Expect = 3.7e-34
Identity = 102/326 (31.29%), Postives = 160/326 (49.08%), Query Frame = 0

Query: 18  VQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVYFERSN--WPKKLSLKLV 77
           +Q  YAG ++F    +  G+ P   +++      + + P      R +      L LK  
Sbjct: 6   IQLCYAGVTLFARATLVHGLSPRVFILYRQAFATIFIFPFLYLSRRKSKIAISSLDLKSF 65

Query: 78  SQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIAWIFRLEKVRLSCIYS 137
           S +FL+S  G+T+ Q+L+L+G+ LTS ++ +A+ N+ P + F I+++   EK+ L  I  
Sbjct: 66  SLIFLVSLIGITINQNLYLEGLYLTSSSMGSAVGNIIPAITFLISFLAGYEKLNLRDIRG 125

Query: 138 KIKILGTFLCVIGAFTMSAMHGAAKDNITQRSPATEHTFNEQK-----ILGCLYLFSAII 197
             KI GT LCV GA +M+ + G    N     P  +      K     ++GCL+LFS+ +
Sbjct: 126 LAKIAGTILCVAGAISMTLLRGPKILNSESALPIAKSVLGHLKDQNTWLIGCLFLFSSTL 185

Query: 198 VLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKLEIGWPLLSVKELAAY 257
             S  ++LQ      +P  +SL A   L G I   VV     +K    W L S  E A  
Sbjct: 186 CWSFWLILQVPISAYYPDNLSLSAWMCLFGTIQCAVVTFFL-EKDPNAWILHSYSEFATC 245

Query: 258 CILGGTISGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVFTWGESINIGSLAGMFL 317
              G   S    +   WA+ KRGPV  ++F+P+ TV   IL+   + E I  GSL G   
Sbjct: 246 LYAGIGASALSFTVQAWAIAKRGPVFSALFNPLCTVIVTILAALFFHEEIYTGSLIGGLG 305

Query: 318 MFTGLYFVLWAKGKERYDNRNGPESE 337
           +  GLY VLW K K+   N++  +++
Sbjct: 306 VILGLYTVLWGKAKDVMMNQDQRDND 330

BLAST of Lag0017476 vs. TAIR 10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 142.1 bits (357), Expect = 8.2e-34
Identity = 112/339 (33.04%), Postives = 167/339 (49.26%), Query Frame = 0

Query: 15  LVGVQFVYAGNSVFMSFLMSLGIDPLTLVIFSTFSTFLIVSPIAVYFERSNWPKKLSLKL 74
           +V +QF YAG S+   F ++ G+ P  L  +      + ++P A + +R   P K++L +
Sbjct: 12  VVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRP-KMTLSI 71

Query: 75  VSQLFLISFGGVTLFQSLFLKGIQLTSPTLATAMPNLAPGMIFAIAWIFRLEKVRLSCIY 134
             ++ L+     T+ Q+L+  G++ TS T   AM N+ P   F +AWIFRLEKV +  I+
Sbjct: 72  FFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNVKKIH 131

Query: 135 SKIKILGTFLCVIGAFTMSAMHG-------AAKDNITQRSPATEHTFNEQKILGCLYLFS 194
           S+ KILGT + V GA  M+ + G       A   +I Q S  T     +    G   +  
Sbjct: 132 SQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQDSSNT--GVKQDLTKGASLIAI 191

Query: 195 AIIVLSSIVVLQASTLGDFPAPMSLCAITSLIGVILTIVVQIVQNQKLEIGWPL-LSVKE 254
             I  +  + LQA TL  +P  +SL A    +G I + +V +   +     W + L  K 
Sbjct: 192 GCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHLDSKL 251

Query: 255 LAAYCILGGTI-SGACVSFNGWAMKKRGPVLVSMFSPIGTVCSLILSVFTWGESINIGSL 314
           LAA  + GG I SG      G  MK RGPV V+ F+P+  V   IL      E + +G +
Sbjct: 252 LAA--VYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMFLGRI 311

Query: 315 AGMFLMFTGLYFVLWAKGKERYDNRNGPESEF-DVDKPL 344
            G  ++  GLY VLW K K+       P S F D+DK L
Sbjct: 312 LGAIVIVLGLYSVLWGKSKDE------PSSSFSDMDKEL 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038906245.11.2e-16488.89WAT1-related protein At5g47470 [Benincasa hispida][more]
XP_008448840.21.3e-15885.19PREDICTED: WAT1-related protein At5g47470 [Cucumis melo][more]
XP_004147210.18.5e-15885.19WAT1-related protein At5g47470 isoform X1 [Cucumis sativus][more]
KAA0041079.16.1e-15685.26WAT1-related protein [Cucumis melo var. makuwa] >TYK12029.1 WAT1-related protein... [more]
XP_011650397.14.5e-15185.16WAT1-related protein At5g47470 isoform X2 [Cucumis sativus] >KAE8650056.1 hypoth... [more]
Match NameE-valueIdentityDescription
Q9FGL02.5e-11260.40WAT1-related protein At5g47470 OS=Arabidopsis thaliana OX=3702 GN=At5g47470 PE=3... [more]
F4JMI75.7e-8849.58WAT1-related protein At4g16620 OS=Arabidopsis thaliana OX=3702 GN=At4g16620 PE=2... [more]
Q8GXB41.4e-3331.69WAT1-related protein At1g09380 OS=Arabidopsis thaliana OX=3702 GN=At1g09380 PE=2... [more]
Q9M0B85.2e-3331.29WAT1-related protein At4g30420 OS=Arabidopsis thaliana OX=3702 GN=At4g30420 PE=3... [more]
O806381.2e-3233.04WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2... [more]
Match NameE-valueIdentityDescription
A0A1S3BKN06.4e-15985.19WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103490881 PE=3 SV=1[more]
A0A5D3CP193.0e-15685.26WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold101... [more]
A0A6J1L2K33.5e-14980.41WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111498520 PE=3 SV=1[more]
A0A0A0L5H51.0e-14885.24WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_3G019970 PE=3 SV=1[more]
A0A6J1DJ668.6e-14880.47WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111021397 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G47470.11.8e-11360.40Nodulin MtN21 /EamA-like transporter family protein [more]
AT4G16620.14.0e-8949.58nodulin MtN21 /EamA-like transporter family protein [more]
AT1G09380.19.7e-3531.69nodulin MtN21 /EamA-like transporter family protein [more]
AT4G30420.13.7e-3431.29nodulin MtN21 /EamA-like transporter family protein [more]
AT2G39510.18.2e-3433.04nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 180..319
e-value: 1.4E-9
score: 38.2
coord: 18..152
e-value: 7.2E-9
score: 35.9
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 10..345
NoneNo IPR availablePANTHERPTHR31218:SF14OS11G0588100 PROTEINcoord: 10..345
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 242..324

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0017476.1Lag0017476.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity