Lag0005112 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0005112
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	calmodulin-like protein 2
Location	chr6: 10833769 .. 10834293 (+)
RNA-Seq Expression	Lag0005112
Synteny	Lag0005112

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTATCTCCAGATTCATTGGGGACTTTCTACAAACACTTGGTGGGCGACCACCACGGGCGGCGGAATCCGAGGGCGGTCCGAGGGCACACAGAGTGGTGTACGACGACGTGGTGGTGAGGGCGTTGATCACGGTGTTCGGGATGAGGGAAAACGGGAGGATCAAGAAGGAAAAGGCGAAGGGGGTGGTGGAGAGGCTGGGGCTGATAGAGAGCGAAGAAGAGAAGGAGAGATTCGAGCTGCCGGCGGAGGGCGGCGACGAGGTGGCGGTGGAGGAGGTGATGGGGGAGGAGGAGGAAGGGAAAAGAAACGAGCTGCTGTGGCAGGCTTTCAAGATATTTGATGTGGATGGAGATGGATTCATTGACAAATTGGAGTTGAAGAGAGTGCTTGAATGTTTGGGGTTGGATAAAGGGTGGGGGATTAGGGAGATTGAGAAAATGGTTAGTGTTGTGGATGTCAATTTGGATGGCAAAGTTGATTTTACTGAGTTTGAATTGATGATGGGTGTTAAATGTTCTTGA

mRNA sequence

ATGGGTATCTCCAGATTCATTGGGGACTTTCTACAAACACTTGGTGGGCGACCACCACGGGCGGCGGAATCCGAGGGCGGTCCGAGGGCACACAGAGTGGTGTACGACGACGTGGTGGTGAGGGCGTTGATCACGGTGTTCGGGATGAGGGAAAACGGGAGGATCAAGAAGGAAAAGGCGAAGGGGGTGGTGGAGAGGCTGGGGCTGATAGAGAGCGAAGAAGAGAAGGAGAGATTCGAGCTGCCGGCGGAGGGCGGCGACGAGGTGGCGGTGGAGGAGGTGATGGGGGAGGAGGAGGAAGGGAAAAGAAACGAGCTGCTGTGGCAGGCTTTCAAGATATTTGATGTGGATGGAGATGGATTCATTGACAAATTGGAGTTGAAGAGAGTGCTTGAATGTTTGGGGTTGGATAAAGGGTGGGGGATTAGGGAGATTGAGAAAATGGTTAGTGTTGTGGATGTCAATTTGGATGGCAAAGTTGATTTTACTGAGTTTGAATTGATGATGGGTGTTAAATGTTCTTGA

Coding sequence (CDS)

ATGGGTATCTCCAGATTCATTGGGGACTTTCTACAAACACTTGGTGGGCGACCACCACGGGCGGCGGAATCCGAGGGCGGTCCGAGGGCACACAGAGTGGTGTACGACGACGTGGTGGTGAGGGCGTTGATCACGGTGTTCGGGATGAGGGAAAACGGGAGGATCAAGAAGGAAAAGGCGAAGGGGGTGGTGGAGAGGCTGGGGCTGATAGAGAGCGAAGAAGAGAAGGAGAGATTCGAGCTGCCGGCGGAGGGCGGCGACGAGGTGGCGGTGGAGGAGGTGATGGGGGAGGAGGAGGAAGGGAAAAGAAACGAGCTGCTGTGGCAGGCTTTCAAGATATTTGATGTGGATGGAGATGGATTCATTGACAAATTGGAGTTGAAGAGAGTGCTTGAATGTTTGGGGTTGGATAAAGGGTGGGGGATTAGGGAGATTGAGAAAATGGTTAGTGTTGTGGATGTCAATTTGGATGGCAAAGTTGATTTTACTGAGTTTGAATTGATGATGGGTGTTAAATGTTCTTGA

Protein sequence

MGISRFIGDFLQTLGGRPPRAAESEGGPRAHRVVYDDVVVRALITVFGMRENGRIKKEKAKGVVERLGLIESEEEKERFELPAEGGDEVAVEEVMGEEEEGKRNELLWQAFKIFDVDGDGFIDKLELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFTEFELMMGVKCS

Homology

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BLAST of Lag0005112 vs. NCBI nr
Match: XP_022951438.1 (calmodulin-like protein 2 [Cucurbita moschata] >XP_023537761.1 calmodulin-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 289.7 bits (740), Expect = 1.7e-74
Identity = 153/179 (85.47%), Postives = 165/179 (92.18%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGRPPRAAESE---GGPRAHRVVYDDVVVRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+GGR  RAA+S    G P  HRV++DDV++RALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTVGGR-SRAADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVERLGLIESEEEKERFELPAE-GGDEVAVEEVMGE-EEEGKRNELLWQAFKIFD 120
           EKAKGVVE+LGLI+SEEEKE FELPA+ GGDEVAVEEV+GE EEEGKRNELLW+AFKIFD
Sbjct: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFD 120

Query: 121 VDGDGFIDKLELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFTEFELMMGVKCS 175
           VDGDGFID +ELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDF EFELMMGVKCS
Sbjct: 121 VDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKCS 178

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BLAST of Lag0005112 vs. NCBI nr
Match: KAG6585405.1 (putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020321.1 putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 287.3 bits (734), Expect = 8.6e-74
Identity = 152/179 (84.92%), Postives = 164/179 (91.62%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGRPPRAAESE---GGPRAHRVVYDDVVVRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+ GR  RAA+S    G P  HRV++DDV++RALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTVSGR-SRAADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVERLGLIESEEEKERFELPAE-GGDEVAVEEVMGE-EEEGKRNELLWQAFKIFD 120
           EKAKGVVE+LGLI+SEEEKE FELPA+ GGDEVAVEEV+GE EEEGKRNELLW+AFKIFD
Sbjct: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFD 120

Query: 121 VDGDGFIDKLELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFTEFELMMGVKCS 175
           VDGDGFID +ELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDF EFELMMGVKCS
Sbjct: 121 VDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKCS 178

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BLAST of Lag0005112 vs. NCBI nr
Match: XP_023002517.1 (calmodulin-like protein 2 [Cucurbita maxima])

HSP 1 Score: 285.4 bits (729), Expect = 3.3e-73
Identity = 152/179 (84.92%), Postives = 164/179 (91.62%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGRPPRAAESE---GGPRAHRVVYDDVVVRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+GGR  RAA+S    G P   RV++DDV++RALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTVGGR-SRAADSAADGGAPSPCRVIHDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVERLGLIESEEEKERFELPAE-GGDEVAVEEVMGE-EEEGKRNELLWQAFKIFD 120
           EKAKGVVE+LGLI+SEEEKE FELPA+ GGDEVAVEEV+GE EEEGKRNELLW+AFKIFD
Sbjct: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFD 120

Query: 121 VDGDGFIDKLELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFTEFELMMGVKCS 175
           VDGDGFID +ELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDF EFELMMGVKCS
Sbjct: 121 VDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKCS 178

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BLAST of Lag0005112 vs. NCBI nr
Match: XP_038886166.1 (calmodulin-like protein 2 [Benincasa hispida])

HSP 1 Score: 284.6 bits (727), Expect = 5.6e-73
Identity = 150/178 (84.27%), Postives = 162/178 (91.01%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGRPPRAAES---EGGPRAHRVVYDDVVVRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT GGR   AAES   +G  R HR+VYDDV++RALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTFGGRSRSAAESTIDDGKTRPHRMVYDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVERLGLIESEEEKERFEL--PAEGGDEVAVEEVMGEEEEGKRNELLWQAFKIFD 120
           EKAKGVVE+LGLI+SEEEK RFEL   AEGGDEVAVEEV+G EEEGKRNELL++AFKIFD
Sbjct: 61  EKAKGVVEKLGLIQSEEEKARFELATAAEGGDEVAVEEVIG-EEEGKRNELLYEAFKIFD 120

Query: 121 VDGDGFIDKLELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFTEFELMMGVKC 174
           VDGDGFID +ELKRVL+CLGLDKGWGIREIEKMVSVVDVNLDGKVDF+EFELMMGVKC
Sbjct: 121 VDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKC 177

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BLAST of Lag0005112 vs. NCBI nr
Match: XP_008445056.1 (PREDICTED: calmodulin-like protein 7 [Cucumis melo] >KAA0065018.1 calmodulin-like protein 7 [Cucumis melo var. makuwa])

HSP 1 Score: 271.2 bits (692), Expect = 6.4e-69
Identity = 145/177 (81.92%), Postives = 159/177 (89.83%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGRPPRAAES---EGGPRAHRVVYDDVVVRALITVFGMRENGRIKK 60
           MGISRFIGDFLQTLGGR   AAES    G  + +R+VYDDV++RALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAAESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVERLGLIESEEEKERFELPA-EGGDEVAVEEVMGEEEEGKRNELLWQAFKIFDV 120
           EKAKGVVE+LGLI   EEKE+FEL A EGGDEVAVEEV+G EEEGKRNELL++AFKIFDV
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELAAGEGGDEVAVEEVIG-EEEGKRNELLYEAFKIFDV 120

Query: 121 DGDGFIDKLELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFTEFELMMGVKC 174
           DGDGFID +ELKRV++CLGLDKGWGIREIEKMVSVVDVNLDGKVDF+EFELMMGVKC
Sbjct: 121 DGDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKC 173

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BLAST of Lag0005112 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.2e-11
Identity = 48/143 (33.57%), Postives = 76/143 (53.15%), Query Frame = 0


Query: 36  DDVVVRALITVFGMRENGRIKKEKAKGVVERLGLIESEEEKERF--ELPAEGGDEVAVEE 95
           D   +  +  +F    +G+I  ++     + LG+I  E+E  +   ++   G   V +EE
Sbjct: 2   DSTELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEE 61

Query: 96  -------VMGEEEEGKRNELLWQAFKIFDVDGDGFIDKLELKRVLECLGLDKGWGIREIE 155
                  +M E+E+    E + +AF +FD +GDGFI   ELK VL  LGL +G  + E  
Sbjct: 62  FGELYKTIMVEDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECR 121

Query: 156 KMVSVVDVNLDGKVDFTEFELMM 170
           KM+  VDV+ DG+V++ EF  MM
Sbjct: 122 KMIMQVDVDGDGRVNYMEFRQMM 144

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BLAST of Lag0005112 vs. ExPASy Swiss-Prot
Match: Q84UL5 (Probable calcium-binding protein CML32 OS=Oryza sativa subsp. japonica OX=39947 GN=CML32 PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.8e-11
Identity = 37/74 (50.00%), Postives = 49/74 (66.22%), Query Frame = 0


Query: 96  GEEEEGKRNELLWQAFKIFDVDGDGFIDKLELKRVLECLGLDKGWGIREIEKMVSVVDVN 155
           G+EEE  R     +AFK+FDVDGDGFI   EL+ VL+ LGL +   +  + +M+  VD N
Sbjct: 120 GDEEEEMR-----EAFKVFDVDGDGFISASELQEVLKKLGLPEAGSLATVREMICNVDRN 179

Query: 156 LDGKVDFTEFELMM 170
            DG+VDF EF+ MM
Sbjct: 180 SDGRVDFGEFKSMM 188

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BLAST of Lag0005112 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 8.2e-11
Identity = 34/63 (53.97%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 107 LWQAFKIFDVDGDGFIDKLELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFTEFE 166
           L +AFK+FD +GDGFI   EL+ VL+ LGL +G  +  +EKM+  VD N DG+VDF EF+
Sbjct: 121 LAEAFKVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFK 180

Query: 167 LMM 170
            MM
Sbjct: 181 NMM 183

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BLAST of Lag0005112 vs. ExPASy Swiss-Prot
Match: Q0JC44 (Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 GN=CML22 PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.4e-10
Identity = 50/173 (28.90%), Postives = 82/173 (47.40%), Query Frame = 0


Query: 18  PPRAAESEGGPRAHRVVYDDVVVRALITVFGMRENGRIKKEKAKGVVERLGL-------- 77
           PP A    G  +  +   D   +  +  +F    +GRI +E+ +  + +LG+        
Sbjct: 71  PPGAGAGAGSKKKQQQQADAAELARVFELFDRNGDGRITREELEDSLGKLGIPVPADELA 130

Query: 78  -------------IESEEEKERFELPAEGGDEVAVEEVMGEEEEGKRNELLWQAFKIFDV 137
                        ++ EE  E +     GGD+   ++   +EEE + +  + +AF++FD 
Sbjct: 131 AVIARIDANGDGCVDVEEFGELYRSIMAGGDD--SKDGRAKEEEEEEDGDMREAFRVFDA 190

Query: 138 DGDGFIDKLELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFTEFELMM 170
           +GDG+I   EL  VL  LGL +G    E  +M+  VD + DG+VDF EF  MM
Sbjct: 191 NGDGYITVDELGAVLASLGLKQGRTAEECRRMIGQVDRDGDGRVDFHEFLQMM 241

[Close]

BLAST of Lag0005112 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.4e-10
Identity = 43/130 (33.08%), Postives = 75/130 (57.69%), Query Frame = 0


Query: 46  VFGMRENGRIKKEKAKGVVERLGLIESEEEKE----RFELPAEGGDEVAVEEVMGEE--E 105
           +F    +G+I K + K   + +G++  E E      + ++  +G  ++     + +E  E
Sbjct: 12  MFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQEMVE 71

Query: 106 EGKRNELLWQAFKIFDVDGDGFIDKLELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGK 165
           E +  E + +AF++FD +GDGFI   EL+ VL  +GL +G  + + +KM+S VDV+ DG 
Sbjct: 72  EKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDVDGDGM 131

Query: 166 VDFTEFELMM 170
           V+F EF+ MM
Sbjct: 132 VNFKEFKQMM 141

The following BLAST results are available for this feature:

BLAST of Lag0005112 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022951438.1	1.7e-74	85.47	calmodulin-like protein 2 [Cucurbita moschata] >XP_023537761.1 calmodulin-like p...	[more]
KAG6585405.1	8.6e-74	84.92	putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023002517.1	3.3e-73	84.92	calmodulin-like protein 2 [Cucurbita maxima]	[more]
XP_038886166.1	5.6e-73	84.27	calmodulin-like protein 2 [Benincasa hispida]	[more]
XP_008445056.1	6.4e-69	81.92	PREDICTED: calmodulin-like protein 7 [Cucumis melo] >KAA0065018.1 calmodulin-lik...	[more]

BLAST of Lag0005112 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZR02	2.2e-11	33.57	Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1	[more]
Q84UL5	2.8e-11	50.00	Probable calcium-binding protein CML32 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SVG9	8.2e-11	53.97	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]
Q0JC44	1.4e-10	28.90	Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SU00	1.4e-10	33.08	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 106..134 e-value: 0.0039 score: 26.4 coord: 144..172 e-value: 1.2 score: 17.1
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 109..169 e-value: 1.2E-10 score: 41.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 140..174 score: 8.962221
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 102..137 score: 15.210791
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 110..165 e-value: 2.34259E-14 score: 62.1801
None	No IPR available	GENE3D	1.10.238.10		coord: 10..172 e-value: 2.3E-19 score: 72.1
None	No IPR available	PANTHER	PTHR10891:SF743	CALCIUM-BINDING PROTEIN CML42	coord: 100..170
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 100..170
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 115..127
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 46..169

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0005112.1	Lag0005112.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding