Lag0005077 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0005077
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein NRT1/ PTR FAMILY 1.2-like
Locationchr6: 10311162 .. 10313702 (+)
RNA-Seq ExpressionLag0005077
SyntenyLag0005077
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCTCTGGAAGACGGGGAGAAGACCAGAATCGTCGAAATCAGTAGCGACGGAGCCAATCAAGCTTCAAATTACAGAAATGGTGGTTTGAAAACCATGCCCTTCATAATCGGTACTCACTTCGATTTCCAATCACTGATTTACTCGAGTTCTTTCAAATTTATAACGAACTCAACCATGCCCTTGGCTTTGCTTTACTTCAATTTGCGACAGTTAATGAGGCATTTGAGAGGGTTGCAAGTTTGGGGCTAACTCCAAACATGGTGTTTTACTTGAGGGATGTCTTCGGGTTTCAAATTGCTGCTGCTTCTATCATTCTGTCTCTGTGGTCTGCTGCTTCCAATGCCTTGGCCATTGTTGGAGCTGTTCTTGCTGATTCTTACTTTGGACGTTTCCGTGTTATAATCCTTGGATCCTTCTGTAGCCTTCTTGTAAGCCTCGTTGATTGTCGAAAATGAACTGTTTCCAATTCAATTGATGTAATAATCTGTTGTGTGGTAGTGTTAAAGAACAGAATCCGTTGGTGATATAGTGCAAGAAACCTTATAGGAACTTAAATGAATAGTATATGAACCTCTAAAGATTCTCAGGGCCTGAAATCCCCTTGCTTGTGTCTTTGACCACTTGATAGTTTAATTTAGATTCCAGCTGCGTGTGTAAGATTATGAAGTTTACAGTTTACCATATTTCTTCAGGGGATGACTCTTCTGTGGTTAACAGCAATGATCCCACAACTGAAATCTTCATCTTGTTCCCTCTCTGGAACATTGTGCAACTCTGGTAATCCTTACCAGCTCGCTGTACTGTTCTCTTCTTTGGCATTTATATCTATTGGAGCTGGTTGCATTAGGCCGTGTTCTATAGCCTTTGGAGCAGACCAACTCACCAATGAGGAAAAGCCGAATAACGAGCGTGTCTTGGATTCCTACTTCAATTGGTATTATGTATCAATTGGAATATCTTCGATTATTTCATTAACTCTAATTGTGTACATTCAAGATAAATTTGGATGGGGGATTGGATTTGCAGTTCCTGCAGGTCTCATGCTTTTTTCTGTGCTAATATTCCTAGTGGGTTCTTCTTTGTACGTGAAAGTGAAGCCTGCACAGAATTTGTTAATTGGGTTATTACAAGTACTTGTTGTGGCCTTTAAGAACCGAAGGTTGTCGCTTCCTCATAGTAACTTTGATCAGTATTACCTAGGCCATGATCCGAAATTTCTCATTCCAACCAATTCTATGAGGTATGTAGGTCAATGTCATGTTCATCTCTATTATTATGATCTTAATTCTTAAGAATATGCATATTACTCTTCTGCTCAGAATGTCTTGATGTCTAAATCATATTCATAGGTGACTTGTTTCTTAAAATAAATAGAGTTGTTTTTTCATTAAAGATTCGTAGGTTTGTGAATGGTGTCTCAGATGCACTATAATATTAACTTTTAGGAGCTATTTGAAAGCTTTTAAACCAATTTTGAAAACAAACACGTAAACTCTTATCCAGGAGAGCTCCTTAAACTGAATTCCTAAAGATTAACACTAAGCATTCTTGACTGTTAACTGTTGGTTCATTCCATATTTCCTTGATATTTGATAATTCCCAATTTTGTTCCTAAGAATATGAAATGTCTGATTTAGTAGTGTTCCCCCTTCTTGTAGGTGTCTGAATAAGGCATGTCTTATAAAAGACGCCGAGAGTAATTTAAATCCAGATGGCTCGGTGTCCAATCCATGGAACCTATGCTCTGTGGATCAAGTTGAGTCACTCAAAGTGTTTCTAAGAATCATCCCAATGTGGTCAACAGGCATTCTCATGCTTGTCTGCTTAAATCAAAATTCATTTGGAACGATCCAAGCCAAGACTATGAACAGACACATTCTCGGTAGTTTCGAAATCCCTGCAGGATCCATCACTATATTCATGGTTGCTTCTTTATCTATATGGATTACGTTTTATGATCGAATAATGGTGCCTTTACTAGCAAGATATGGTCATGCTGGAGGACTCAGTCCTCAATTAAGAATGGGAATCGGTTTGCTTCTCTCGAGCTTCGCAATGGCAATTTCAGCCATCGTTGCAGGTACAAGGCGTAGGCTTGCCATAGAAGAAGGTTTAGAAGACCAACCAAATGCTGTCATTGACATGTCAGCTCTATGGATGGTACCTCAATGTGCAGTCCTGGGAGTTGCTGAAGCTTTGAACTCGGTCGGACAGCTGGAGTATTTCTACTTGCGGGTGCCAAAGACATTGTCTAGCGTTGCAGTTGCTCTATACACAGTTGAGATGGCTGTAGCTAACTTGGTGGGAAGCATTCTTGTGCAGATAGTAAATGGCATAACAGGAGAAGGAAATAAGACAAGCTGGTTGGACAACAATATCAACAAGGGTCACCTGGATTACTTTTACTGGTTATGTGCTGCATTGGCTTTGATAAACTTTTTTGGTTATCTTATATTTTGTTGGGCTTATGAGAGACAGGGCAATGAATCAAATGAGATGGAAGACTTTGAGTATAGGAACTTACCTTCATCTTAG

mRNA sequence

ATGGAGGCTCTGGAAGACGGGGAGAAGACCAGAATCGTCGAAATCAGTAGCGACGGAGCCAATCAAGCTTCAAATTACAGAAATGGTGGTTTGAAAACCATGCCCTTCATAATCGTTAATGAGGCATTTGAGAGGGTTGCAAGTTTGGGGCTAACTCCAAACATGGTGTTTTACTTGAGGGATGTCTTCGGGTTTCAAATTGCTGCTGCTTCTATCATTCTGTCTCTGTGGTCTGCTGCTTCCAATGCCTTGGCCATTGTTGGAGCTGTTCTTGCTGATTCTTACTTTGGACGTTTCCGTGTTATAATCCTTGGATCCTTCTGTAGCCTTCTTGGGATGACTCTTCTGTGGTTAACAGCAATGATCCCACAACTGAAATCTTCATCTTGTTCCCTCTCTGGAACATTGTGCAACTCTGGTAATCCTTACCAGCTCGCTGTACTGTTCTCTTCTTTGGCATTTATATCTATTGGAGCTGGTTGCATTAGGCCGTGTTCTATAGCCTTTGGAGCAGACCAACTCACCAATGAGGAAAAGCCGAATAACGAGCGTGTCTTGGATTCCTACTTCAATTGGTATTATGTATCAATTGGAATATCTTCGATTATTTCATTAACTCTAATTGTGTACATTCAAGATAAATTTGGATGGGGGATTGGATTTGCAGTTCCTGCAGGTCTCATGCTTTTTTCTGTGCTAATATTCCTAGTGGGTTCTTCTTTGTACGTGAAAGTGAAGCCTGCACAGAATTTGTTAATTGGGTTATTACAAGTACTTGTTGTGGCCTTTAAGAACCGAAGGTTGTCGCTTCCTCATAGTAACTTTGATCAGTATTACCTAGGCCATGATCCGAAATTTCTCATTCCAACCAATTCTATGAGGTGTCTGAATAAGGCATGTCTTATAAAAGACGCCGAGAGTAATTTAAATCCAGATGGCTCGGTGTCCAATCCATGGAACCTATGCTCTGTGGATCAAGTTGAGTCACTCAAAGTGTTTCTAAGAATCATCCCAATGTGGTCAACAGGCATTCTCATGCTTGTCTGCTTAAATCAAAATTCATTTGGAACGATCCAAGCCAAGACTATGAACAGACACATTCTCGGTAGTTTCGAAATCCCTGCAGGATCCATCACTATATTCATGGTTGCTTCTTTATCTATATGGATTACGTTTTATGATCGAATAATGGTGCCTTTACTAGCAAGATATGGTCATGCTGGAGGACTCAGTCCTCAATTAAGAATGGGAATCGGTTTGCTTCTCTCGAGCTTCGCAATGGCAATTTCAGCCATCGTTGCAGGTACAAGGCGTAGGCTTGCCATAGAAGAAGGTTTAGAAGACCAACCAAATGCTGTCATTGACATGTCAGCTCTATGGATGGTACCTCAATGTGCAGTCCTGGGAGTTGCTGAAGCTTTGAACTCGGTCGGACAGCTGGAGTATTTCTACTTGCGGGTGCCAAAGACATTGTCTAGCGTTGCAGTTGCTCTATACACAGTTGAGATGGCTGTAGCTAACTTGGTGGGAAGCATTCTTGTGCAGATAGTAAATGGCATAACAGGAGAAGGAAATAAGACAAGCTGGTTGGACAACAATATCAACAAGGGTCACCTGGATTACTTTTACTGGTTATGTGCTGCATTGGCTTTGATAAACTTTTTTGGTTATCTTATATTTTGTTGGGCTTATGAGAGACAGGGCAATGAATCAAATGAGATGGAAGACTTTGAGTATAGGAACTTACCTTCATCTTAG

Coding sequence (CDS)

ATGGAGGCTCTGGAAGACGGGGAGAAGACCAGAATCGTCGAAATCAGTAGCGACGGAGCCAATCAAGCTTCAAATTACAGAAATGGTGGTTTGAAAACCATGCCCTTCATAATCGTTAATGAGGCATTTGAGAGGGTTGCAAGTTTGGGGCTAACTCCAAACATGGTGTTTTACTTGAGGGATGTCTTCGGGTTTCAAATTGCTGCTGCTTCTATCATTCTGTCTCTGTGGTCTGCTGCTTCCAATGCCTTGGCCATTGTTGGAGCTGTTCTTGCTGATTCTTACTTTGGACGTTTCCGTGTTATAATCCTTGGATCCTTCTGTAGCCTTCTTGGGATGACTCTTCTGTGGTTAACAGCAATGATCCCACAACTGAAATCTTCATCTTGTTCCCTCTCTGGAACATTGTGCAACTCTGGTAATCCTTACCAGCTCGCTGTACTGTTCTCTTCTTTGGCATTTATATCTATTGGAGCTGGTTGCATTAGGCCGTGTTCTATAGCCTTTGGAGCAGACCAACTCACCAATGAGGAAAAGCCGAATAACGAGCGTGTCTTGGATTCCTACTTCAATTGGTATTATGTATCAATTGGAATATCTTCGATTATTTCATTAACTCTAATTGTGTACATTCAAGATAAATTTGGATGGGGGATTGGATTTGCAGTTCCTGCAGGTCTCATGCTTTTTTCTGTGCTAATATTCCTAGTGGGTTCTTCTTTGTACGTGAAAGTGAAGCCTGCACAGAATTTGTTAATTGGGTTATTACAAGTACTTGTTGTGGCCTTTAAGAACCGAAGGTTGTCGCTTCCTCATAGTAACTTTGATCAGTATTACCTAGGCCATGATCCGAAATTTCTCATTCCAACCAATTCTATGAGGTGTCTGAATAAGGCATGTCTTATAAAAGACGCCGAGAGTAATTTAAATCCAGATGGCTCGGTGTCCAATCCATGGAACCTATGCTCTGTGGATCAAGTTGAGTCACTCAAAGTGTTTCTAAGAATCATCCCAATGTGGTCAACAGGCATTCTCATGCTTGTCTGCTTAAATCAAAATTCATTTGGAACGATCCAAGCCAAGACTATGAACAGACACATTCTCGGTAGTTTCGAAATCCCTGCAGGATCCATCACTATATTCATGGTTGCTTCTTTATCTATATGGATTACGTTTTATGATCGAATAATGGTGCCTTTACTAGCAAGATATGGTCATGCTGGAGGACTCAGTCCTCAATTAAGAATGGGAATCGGTTTGCTTCTCTCGAGCTTCGCAATGGCAATTTCAGCCATCGTTGCAGGTACAAGGCGTAGGCTTGCCATAGAAGAAGGTTTAGAAGACCAACCAAATGCTGTCATTGACATGTCAGCTCTATGGATGGTACCTCAATGTGCAGTCCTGGGAGTTGCTGAAGCTTTGAACTCGGTCGGACAGCTGGAGTATTTCTACTTGCGGGTGCCAAAGACATTGTCTAGCGTTGCAGTTGCTCTATACACAGTTGAGATGGCTGTAGCTAACTTGGTGGGAAGCATTCTTGTGCAGATAGTAAATGGCATAACAGGAGAAGGAAATAAGACAAGCTGGTTGGACAACAATATCAACAAGGGTCACCTGGATTACTTTTACTGGTTATGTGCTGCATTGGCTTTGATAAACTTTTTTGGTTATCTTATATTTTGTTGGGCTTATGAGAGACAGGGCAATGAATCAAATGAGATGGAAGACTTTGAGTATAGGAACTTACCTTCATCTTAG

Protein sequence

MEALEDGEKTRIVEISSDGANQASNYRNGGLKTMPFIIVNEAFERVASLGLTPNMVFYLRDVFGFQIAAASIILSLWSAASNALAIVGAVLADSYFGRFRVIILGSFCSLLGMTLLWLTAMIPQLKSSSCSLSGTLCNSGNPYQLAVLFSSLAFISIGAGCIRPCSIAFGADQLTNEEKPNNERVLDSYFNWYYVSIGISSIISLTLIVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSSLYVKVKPAQNLLIGLLQVLVVAFKNRRLSLPHSNFDQYYLGHDPKFLIPTNSMRCLNKACLIKDAESNLNPDGSVSNPWNLCSVDQVESLKVFLRIIPMWSTGILMLVCLNQNSFGTIQAKTMNRHILGSFEIPAGSITIFMVASLSIWITFYDRIMVPLLARYGHAGGLSPQLRMGIGLLLSSFAMAISAIVAGTRRRLAIEEGLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQLEYFYLRVPKTLSSVAVALYTVEMAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLDYFYWLCAALALINFFGYLIFCWAYERQGNESNEMEDFEYRNLPSS
Homology
BLAST of Lag0005077 vs. NCBI nr
Match: XP_022962308.1 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 975.3 bits (2520), Expect = 2.3e-280
Identity = 498/585 (85.13%), Postives = 532/585 (90.94%), Query Frame = 0

Query: 1   MEALEDGEKTRIVEISSDGANQASNYRNGGLKTMPFIIVNEAFERVASLGLTPNMVFYLR 60
           ME LEDGEKT I    S+ A QASNY NGGLKTMPFIIVNE FERVASLGLTPNMVFYLR
Sbjct: 1   MEVLEDGEKTGIAASGSEEAEQASNYTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DVFGFQIAAASIILSLWSAASNALAIVGAVLADSYFGRFRVIILGSFCSLLGMTLLWLTA 120
           DVFGF+IAAASIILSLWSAASNALAIVGAVLADSY GRF VIILGS  SLLGM LLWLTA
Sbjct: 61  DVFGFEIAAASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 MIPQLKSSSCSLSGTLCNSGNPYQLAVLFSSLAFISIGAGCIRPCSIAFGADQLTNEEKP 180
           +IPQL+ +SCS SG LC+S NPYQLAVLFSSL FISIGAGCIRPCS+AFGADQLT EEKP
Sbjct: 121 IIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 NNERVLDSYFNWYYVSIGISSIISLTLIVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSS 240
           NNE VLDSYFNWYY SIGI++II+L+LIV+IQDKFGWGIGFAVPA LMLFSVLIFLVGSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIIALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPAQNLLIGLLQVLVVAFKNRRLSLPHSNFDQYYLGHDPKFLIPTNSMRCLNKAC 300
           LYVKVKP Q+LL G L+V+VVAFKNR+LSLP SNFDQYYLG DPK  IPTNSMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCRIPTNSMRYLNKAC 300

Query: 301 LIKDAESNLNPDGSVSNPWNLCSVDQVESLKVFLRIIPMWSTGILMLVCLNQNSFGTIQA 360
           LIKDA++NLNPDGSVSNPWNLCSVDQVESLK FLRIIPMWSTGILMLVCLNQNSFGTIQA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 KTMNRHILGSFEIPAGSITIFMVASLSIWITFYDRIMVPLLARYGHAGGLSPQLRMGIGL 420
           KTMNRHI  SFEIPAGSITIFMV SL  WI FYDRI+VPLLAR+GHAGGLSP+LR+GIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSPKLRIGIGL 420

Query: 421 LLSSFAMAISAIVAGTRRRLAIEEGLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQL 480
           LLS FAMAISA VA  RRR+AIE+G EDQPNAVI+MSALWMVPQC VLGVAEALNSVGQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFYLRVPKTLSSVAVALYTVEMAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLD 540
           EYFY ++PKTLSSVAVALY+ EMAVANLVGSI+VQ+VN ITGEGNKTSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALINFFGYLIFCWAYERQGNESNEMEDFEYRNLPSS 586
           YFYWLCAALALINFFGYL++CWA  RQ  E NEMEDFEYRNLPSS
Sbjct: 541 YFYWLCAALALINFFGYLVYCWALGRQDKEPNEMEDFEYRNLPSS 585

BLAST of Lag0005077 vs. NCBI nr
Match: KAG6598782.1 (Protein NRT1/ PTR FAMILY 1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 972.6 bits (2513), Expect = 1.5e-279
Identity = 496/585 (84.79%), Postives = 530/585 (90.60%), Query Frame = 0

Query: 1   MEALEDGEKTRIVEISSDGANQASNYRNGGLKTMPFIIVNEAFERVASLGLTPNMVFYLR 60
           ME LEDGEK+ I    S+GA QASNY NGGLKTMPFIIVNE FERVASLGLTPNMVFYLR
Sbjct: 1   MEVLEDGEKSGIAASGSEGAEQASNYTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DVFGFQIAAASIILSLWSAASNALAIVGAVLADSYFGRFRVIILGSFCSLLGMTLLWLTA 120
           DVFGF+IAAASIILSLWSAASNALAIVGAVLADSY GRF VIILGS  SLLGM LLWLTA
Sbjct: 61  DVFGFEIAAASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 MIPQLKSSSCSLSGTLCNSGNPYQLAVLFSSLAFISIGAGCIRPCSIAFGADQLTNEEKP 180
           +IPQL+  SCS SG LC+S NPYQLAVLFSSL FISIGAGCIRPCS+AFGADQLT EEKP
Sbjct: 121 IIPQLRPESCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 NNERVLDSYFNWYYVSIGISSIISLTLIVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSS 240
           NNE VLDSYFNWYY SIGI++I++L+LIV+IQDKFGWGIGFAVPA LMLFSVLIFLVGSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIVALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPAQNLLIGLLQVLVVAFKNRRLSLPHSNFDQYYLGHDPKFLIPTNSMRCLNKAC 300
           LYVKVKP Q+LL G L+V+VVAFKNR+LSLP SNFDQYYLG DPK  IPTNSMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCRIPTNSMRYLNKAC 300

Query: 301 LIKDAESNLNPDGSVSNPWNLCSVDQVESLKVFLRIIPMWSTGILMLVCLNQNSFGTIQA 360
           LIKDA++NLNPDGSVSNPWNLCSVDQVESLK FLRIIPMWSTGILMLVCLNQNSFGTIQA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 KTMNRHILGSFEIPAGSITIFMVASLSIWITFYDRIMVPLLARYGHAGGLSPQLRMGIGL 420
           KTMNRHI  SFEIPAGSITIFMV SL  WI FYDRI+VPLLAR+GHAGGLSP+LR+GIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSPKLRIGIGL 420

Query: 421 LLSSFAMAISAIVAGTRRRLAIEEGLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQL 480
           LLS FAMAISA VA  RRR+AIE+G EDQPNAVI+MSALWMVPQC VLGVAEALNSVGQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFYLRVPKTLSSVAVALYTVEMAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLD 540
           EYFY ++PKTLSSVAVALY+ EM VANLVGSI+VQ+VN ITGEGNKTSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYSFEMVVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALINFFGYLIFCWAYERQGNESNEMEDFEYRNLPSS 586
           YFYWLCAALALINFFGYL+ CWA  RQ  E NEMEDFEYRNLPSS
Sbjct: 541 YFYWLCAALALINFFGYLVCCWALGRQDKEPNEMEDFEYRNLPSS 585

BLAST of Lag0005077 vs. NCBI nr
Match: XP_023545603.1 (protein NRT1/ PTR FAMILY 1.2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 970.7 bits (2508), Expect = 5.7e-279
Identity = 495/585 (84.62%), Postives = 531/585 (90.77%), Query Frame = 0

Query: 1   MEALEDGEKTRIVEISSDGANQASNYRNGGLKTMPFIIVNEAFERVASLGLTPNMVFYLR 60
           ME LEDGEKT I    S+GA QASNY NGGLKTMPFIIVNE FERVASLGLTPNMVFYLR
Sbjct: 1   MEVLEDGEKTGIAVSGSEGAEQASNYTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DVFGFQIAAASIILSLWSAASNALAIVGAVLADSYFGRFRVIILGSFCSLLGMTLLWLTA 120
           DVFGFQIA ASIILSLWSAASNALAIVGAVLADSY GRF VIILGS  SLLGM LLWLTA
Sbjct: 61  DVFGFQIADASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 MIPQLKSSSCSLSGTLCNSGNPYQLAVLFSSLAFISIGAGCIRPCSIAFGADQLTNEEKP 180
           +IPQL+ +SCS SG LC+S NPYQLAVLFSSL FISIGAGCIRPCS+AFGADQLT EEKP
Sbjct: 121 IIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 NNERVLDSYFNWYYVSIGISSIISLTLIVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSS 240
           NN+ VLDSYFNWYY SIGI++II+L+LIV+IQD+FGWGIGFAVPA LMLFSVLIFLVGSS
Sbjct: 181 NNDSVLDSYFNWYYASIGIATIIALSLIVFIQDQFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPAQNLLIGLLQVLVVAFKNRRLSLPHSNFDQYYLGHDPKFLIPTNSMRCLNKAC 300
           LYVKVKP Q+LL G L+V+VVAFKNR+LSLP SNFDQYYLG DPK  IPT+SMRCLNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCPIPTDSMRCLNKAC 300

Query: 301 LIKDAESNLNPDGSVSNPWNLCSVDQVESLKVFLRIIPMWSTGILMLVCLNQNSFGTIQA 360
           LIKD ++NLNPDGSVSNPWNLCSVDQVESLK FLRIIPMWSTGILMLVCLNQNSFGTIQA
Sbjct: 301 LIKDGKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 KTMNRHILGSFEIPAGSITIFMVASLSIWITFYDRIMVPLLARYGHAGGLSPQLRMGIGL 420
           KTMNRHI  +FEIPAGSITIFMV SL  WI FYDRI+VPLLAR+GHAGGLSP+LR+GIGL
Sbjct: 361 KTMNRHITRTFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSPKLRIGIGL 420

Query: 421 LLSSFAMAISAIVAGTRRRLAIEEGLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQL 480
           LLS FAMAISA VA  RRR+AIE+G EDQPNAVI+MSALWMVPQC VLGVAEALNSVGQ+
Sbjct: 421 LLSFFAMAISAGVAYIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFYLRVPKTLSSVAVALYTVEMAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLD 540
           EYFY ++PKTLSSVAVALY+ EMAVANLVGSI+VQ+VN ITGEGNKTSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALINFFGYLIFCWAYERQGNESNEMEDFEYRNLPSS 586
           YFYWLCAALALINFFGYL+ CWA  RQ  E NEMEDFEYRNLPSS
Sbjct: 541 YFYWLCAALALINFFGYLVCCWALGRQHKEPNEMEDFEYRNLPSS 585

BLAST of Lag0005077 vs. NCBI nr
Match: XP_022996584.1 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 967.6 bits (2500), Expect = 4.8e-278
Identity = 496/585 (84.79%), Postives = 528/585 (90.26%), Query Frame = 0

Query: 1   MEALEDGEKTRIVEISSDGANQASNYRNGGLKTMPFIIVNEAFERVASLGLTPNMVFYLR 60
           ME LEDGEKT I    S+G  QASN+ NGGLKTMPFIIVNE FERVASLGLTPNMVFYLR
Sbjct: 1   MEVLEDGEKTGISASGSEGTEQASNHTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DVFGFQIAAASIILSLWSAASNALAIVGAVLADSYFGRFRVIILGSFCSLLGMTLLWLTA 120
           DVFGFQIA ASIILSLWSAASNALAIVGAVLADSY GRF VIILGS  SLLGM LLWLTA
Sbjct: 61  DVFGFQIADASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 MIPQLKSSSCSLSGTLCNSGNPYQLAVLFSSLAFISIGAGCIRPCSIAFGADQLTNEEKP 180
           +IPQL+ +SCS SG LC+S NPYQLAVLFSSL FISIGAGCIRPCS+AFGADQLT EEKP
Sbjct: 121 IIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 NNERVLDSYFNWYYVSIGISSIISLTLIVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSS 240
           NNE VLDSYFNWYY SIGI++I +L+LIV+IQDKFGWGIGFAVPA LMLFSVLIFLVGSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIFALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPAQNLLIGLLQVLVVAFKNRRLSLPHSNFDQYYLGHDPKFLIPTNSMRCLNKAC 300
           LYVKVKP Q+LL G L+V+VVAFKNR+LSLP SNFDQYYLG DPK  IPTNSMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGCDPKCPIPTNSMRYLNKAC 300

Query: 301 LIKDAESNLNPDGSVSNPWNLCSVDQVESLKVFLRIIPMWSTGILMLVCLNQNSFGTIQA 360
           LIKDA++NLNPDGSVSNPWNLCSVDQVESLK FLRIIPMWSTGILMLVCLNQNSFGTIQA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 KTMNRHILGSFEIPAGSITIFMVASLSIWITFYDRIMVPLLARYGHAGGLSPQLRMGIGL 420
           KTMNRHI  SFEIPAGSITIFMV SL  WI FYDRI+VPLLARYGHAGGLSP+LR+GIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARYGHAGGLSPKLRIGIGL 420

Query: 421 LLSSFAMAISAIVAGTRRRLAIEEGLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQL 480
           LLS FAMAISA VA  RRR+AIE+G EDQPNAVI+MSALWMVPQC VLGVAEALNSVGQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFYLRVPKTLSSVAVALYTVEMAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLD 540
           EYFY ++PK LSSVAVALY+ EMAVANLVGSI+VQ+VN ITGEGNKTSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKMLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALINFFGYLIFCWAYERQGNESNEMEDFEYRNLPSS 586
           YFYWLCAALALINFFGYL+ CWA  RQ  E NEMEDFEYRNLPSS
Sbjct: 541 YFYWLCAALALINFFGYLVCCWALGRQDKEPNEMEDFEYRNLPSS 585

BLAST of Lag0005077 vs. NCBI nr
Match: XP_022132097.1 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Momordica charantia])

HSP 1 Score: 940.6 bits (2430), Expect = 6.3e-270
Identity = 475/589 (80.65%), Postives = 527/589 (89.47%), Query Frame = 0

Query: 1   MEALEDGEKTRIVEISSDGANQASNYRNGGLKTMPFIIVNEAFERVASLGLTPNMVFYLR 60
           ME +E+ EKT I + SS+G +QASN R GGL+TMPFIIVNE FE++ASLGLTPNMVFYLR
Sbjct: 1   MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR 60

Query: 61  DVFGFQIAAASIILSLWSAASNALAIVGAVLADSYFGRFRVIILGSFCSLLGMTLLWLTA 120
           D +GFQIAAAS ILSLWSAASNALAI GAVLADSY GRFR IILGSF S LGM LLWLTA
Sbjct: 61  DDYGFQIAAASSILSLWSAASNALAIAGAVLADSYVGRFRSIILGSFSSFLGMALLWLTA 120

Query: 121 MIPQLKSSSCSLSGTLCNSGNPYQLAVLFSSLAFISIGAGCIRPCSIAFGADQLTNEEKP 180
           MIPQLK SSCS SG LC+S NPYQLA+LF+SL FISIGAGCIRPCSIAFGADQLTNE+KP
Sbjct: 121 MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP 180

Query: 181 NNERVLDSYFNWYYVSIGISSIISLTLIVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSS 240
           NNE VLD+YFNWYY S GI+S+I+LTLIVYIQD+FGW  GFAVPA LMLFSVLIFLVGSS
Sbjct: 181 NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPAQNLLIGLLQVLVVAFKNRRLSLPHSNFDQYYLGHDPKFLIPTNSMRCLNKAC 300
           LYVKVKP Q+LL GLLQV+VVAFKNRRLSLP+SN+DQYYLG DPKFL+PT+SMRCLNKAC
Sbjct: 241 LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC 300

Query: 301 LIKDAESNLNPDGSVSNPWNLCSVDQVESLKVFLRIIPMWSTGILMLVCLNQNSFGTIQA 360
           LIKDAE++LNPDGS+SNPWNLCSV+ VESLK FLRIIPMWSTGILMLVCLNQNSFGT+QA
Sbjct: 301 LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA 360

Query: 361 KTMNRHILGSFEIPAGSITIFMVASLSIWITFYDRIMVPLLARYGHAGGLSPQLRMGIGL 420
           KTMNRHI GSFEIPAGSI + M+ SLS+W+ FYDRIMVPLL R GHAGGLSP+LRMGIGL
Sbjct: 361 KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL 420

Query: 421 LLSSFAMAISAIVAGTRRRLAIEEGLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQL 480
           LLS FAMAISA+V GTRRR+A EEGLEDQP AVIDMS LW+VPQC VLGVAE  N++GQ+
Sbjct: 421 LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI 480

Query: 481 EYFYLRVPKTLSSVAVALYTVEMAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLD 540
           EYFY ++PKTLSSVAVALYTVEMAVA+LVGS LVQ+VNGITG+GN+TSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD 540

Query: 541 YFYWLCAALALINFFGYLIFCWAY----ERQGNESNEMEDFEYRNLPSS 586
           YFYWLCAALAL+NF GYL+    Y     R+ NESNE++DFEYR LPSS
Sbjct: 541 YFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 589

BLAST of Lag0005077 vs. ExPASy Swiss-Prot
Match: Q9M817 (Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 4.4e-149
Identity = 268/544 (49.26%), Postives = 376/544 (69.12%), Query Frame = 0

Query: 29  GGLKTMPFIIVNEAFERVASLGLTPNMVFYLRDVFGFQIAAASIILSLWSAASNALAIVG 88
           GG+ TMPFII NEAFE+VAS GL PNM+ YL   + F +A  + +L +WSAASN   ++G
Sbjct: 23  GGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAASNFTPLLG 82

Query: 89  AVLADSYFGRFRVIILGSFCSLLGMTLLWLTAMIPQLKSSSC--SLSGTLCNSGNPYQLA 148
           A L+DSY GRF  I + S  S LGM LLWLTAM+PQ+K S C  + +G+ C S    QLA
Sbjct: 83  AFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCGSSTASQLA 142

Query: 149 VLFSSLAFISIGAGCIRPCSIAFGADQLTNEEKPNNERVLDSYFNWYYVSIGISSIISLT 208
           +L+S+ A ISIG+G IRPCS+AFGADQL N+E P NERVL+S+F WYY S  ++ +I+ T
Sbjct: 143 LLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSAVAVLIAFT 202

Query: 209 LIVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSSLYVKVKPAQNLLIGLLQVLVVAFKNR 268
            IVYIQ+  GW IGF VPA LML + L+F++ S LYV     ++L  GL Q +V A+K R
Sbjct: 203 GIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQAIVAAYKKR 262

Query: 269 RLSLP--HSNFDQYYLGHDPKFLIPTNSMRCLNKACLIKDAESNLNPDGSVSNPWNLCSV 328
           +LSLP  H +FD YY   D +   P+  +R LNKACLI + E  +  DG   NPW LC+ 
Sbjct: 263 KLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFALNPWRLCTT 322

Query: 329 DQVESLKVFLRIIPMWSTGILMLVCLNQNSFGTIQAKTMNRHIL---GSFEIPAGSITIF 388
           D+VE LK  +++IP+WSTGI+M +  +Q+SF  +QA +M+R +     SF++PAGS  +F
Sbjct: 323 DKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQVPAGSFGMF 382

Query: 389 MVASLSIWITFYDRIMVPLLARY-GHAGGLSPQLRMGIGLLLSSFAMAISAIVAGTRRRL 448
            + +L++W+  YDR ++PL ++  G    LS +LRMG+GL +S  AMAISA+V   RR+ 
Sbjct: 383 TIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAMVESFRRKK 442

Query: 449 AIEEGLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQLEYFYLRVPKTLSSVAVALYT 508
           AI +G  +  NAV+D+SA+W+VPQ  + G+AEAL ++GQ E+FY   PK++SS+A +L+ 
Sbjct: 443 AISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMSSIAASLFG 502

Query: 509 VEMAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLDYFYWLCAALALINFFGYLIF 565
           + MAVA+L+ S+++  VN +T    K SW+ +NINKGH +Y+YW+ A ++ IN   Y+I 
Sbjct: 503 LGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFINVIYYVIC 562

BLAST of Lag0005077 vs. ExPASy Swiss-Prot
Match: Q8LPL2 (Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2)

HSP 1 Score: 507.7 bits (1306), Expect = 1.8e-142
Identity = 255/542 (47.05%), Postives = 370/542 (68.27%), Query Frame = 0

Query: 29  GGLKTMPFIIVNEAFERVASLGLTPNMVFYLRDVFGFQIAAASIILSLWSAASNALAIVG 88
           GGL TMPFII NE FE+VAS GL  NM+ YL   +   +     +L +W AA+N + +VG
Sbjct: 25  GGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVKGQTVLFMWVAATNFMPLVG 84

Query: 89  AVLADSYFGRFRVIILGSFCSLLGMTLLWLTAMIPQLKSSSC-SLSGTLCNSGNPYQLAV 148
           A L+DSY GRF  I++ S  SLLGM +LWLTAM+PQ+K S C + +GT C+S    QLA+
Sbjct: 85  AFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLAL 144

Query: 149 LFSSLAFISIGAGCIRPCSIAFGADQLTNEEKPNNERVLDSYFNWYYVSIGISSIISLTL 208
           L+++ A ISIG+G IRPCS+AFGADQL N+E P NERVL+S+F WYY S  ++ +I+ T+
Sbjct: 145 LYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFTV 204

Query: 209 IVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSSLYVKVKPAQNLLIGLLQVLVVAFKNRR 268
           IVYIQD  GW IGF +PA LML +  +F+  S LYVK   +++L  GL QV+  A+  R 
Sbjct: 205 IVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKRN 264

Query: 269 LSLP--HSNFDQYYLGHDPKFLIPTNSMRCLNKACLIKDAESNLNPDGSVSNPWNLCSVD 328
           L+LP  H + D YY   D +   P++ +R LNKAC I + + +L  DG   N W LC+ D
Sbjct: 265 LTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAISNRDEDLGSDGLALNQWRLCTTD 324

Query: 329 QVESLKVFLRIIPMWSTGILMLVCLNQNSFGTIQAKTMNRHIL--GSFEIPAGSITIFMV 388
           QVE LK  +++IP+WSTGI+M + ++QNSF  +QAK+M+R +    +F+IPAGS  +F +
Sbjct: 325 QVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMFTI 384

Query: 389 ASLSIWITFYDRIMVPLLARY-GHAGGLSPQLRMGIGLLLSSFAMAISAIVAGTRRRLAI 448
            +L  W+  YDR ++PL ++  G    ++ ++RMG+GL +S  AMA+SA V   RR+ AI
Sbjct: 385 IALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKTAI 444

Query: 449 EEGLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQLEYFYLRVPKTLSSVAVALYTVE 508
            +GL +  N+ + +SA+W+VPQ  + G+AEAL  +GQ E+FY   PK++SS+A +L+ + 
Sbjct: 445 SQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFGLG 504

Query: 509 MAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLDYFYWLCAALALINFFGYLIFCW 565
           MAVAN++ S+++  V   + +GN  SW+++NINKGH DY+YW+ A L+ +N   Y++  W
Sbjct: 505 MAVANILASVILNAVKNSSKQGN-VSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVCSW 564

BLAST of Lag0005077 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 4.6e-106
Identity = 213/562 (37.90%), Postives = 336/562 (59.79%), Query Frame = 0

Query: 29  GGLKTMPFIIVNEAFERVASLGLTPNMVFYLRDVFGFQIAAASIILSLWSAASNALAIVG 88
           GG + + FI+ NE  ER+ S+GL  N + YL  VF  +   A+ ++++WS  +N   +VG
Sbjct: 53  GGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVG 112

Query: 89  AVLADSYFGRFRVIILGSFCSLLGMTLLWLTAMIPQLKSSSCSLSGTL-CNSGNPYQLAV 148
           A ++D+Y GRF+ I   SF +LLG+  + LTA  PQL  +SC+    L C   N  Q+ V
Sbjct: 113 AYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGV 172

Query: 149 LFSSLAFISIGAGCIRPCSIAFGADQLTNEEKPNNERVLDSYFNWYYVSIGISSIISLTL 208
           L   L F+S+G+G IRPCSI FG DQ  ++      + + S+FNWYY++  +  II+ T+
Sbjct: 173 LLLGLCFLSVGSGGIRPCSIPFGVDQF-DQRTEEGVKGVASFFNWYYMTFTVVLIITQTV 232

Query: 209 IVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSSLYVKVKPAQNLLIGLLQVLVVAFKNRR 268
           +VYIQD+  W IGF++P GLM  +V++F  G   YV VKP  ++  G+ QV+V A K R+
Sbjct: 233 VVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRK 292

Query: 269 LSLPHSNFDQYYLGHDPKF-------LIPTNSMRCLNKACLIKDAESNLNPDGSVSNPWN 328
           L LP  + D     +DP         L  +N  RCL+KA ++   E +L P+G  ++ W 
Sbjct: 293 LKLPAED-DGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADKWR 352

Query: 329 LCSVDQVESLKVFLRIIPMWSTGILMLVCL-NQNSFGTIQAKTMNRHILGSFEIPAGSIT 388
           LCSV +VE +K  +RI+P+WS GI+ L  +  Q +F   QA  M+R++   FEIPAGS++
Sbjct: 353 LCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLS 412

Query: 389 IFMVASLSIWITFYDRIMVPLLARY-GHAGGLSPQLRMGIGLLLSSFAMAISAIVAGTRR 448
           +  + ++ I++ FYDR+ VP + R  GH  G++   R+G G++ + F+M ++ IV   RR
Sbjct: 413 VISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRR 472

Query: 449 RLAIEEGLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQLEYFYLRVPKTLSSVAVAL 508
             +I  G    P  +  MS  W+ PQ  ++G+ EA N +GQ+E+F  + P+ + S+A +L
Sbjct: 473 IRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSL 532

Query: 509 YTVEMAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLDYFYWLCAALALINFFGYL 568
           +++  A ++ + S LV +V+  +G  ++  WL+ N+N G LDYFY+L A L ++N    L
Sbjct: 533 FSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVN----L 592

Query: 569 IFCWAYER--QGNESNEMEDFE 579
           ++ W   R  +      +EDFE
Sbjct: 593 VYFWYCARGYRYKVGLPIEDFE 603

BLAST of Lag0005077 vs. ExPASy Swiss-Prot
Match: Q9M9V7 (Protein NRT1/ PTR FAMILY 2.9 OS=Arabidopsis thaliana OX=3702 GN=NPF2.9 PE=1 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 3.5e-98
Identity = 205/539 (38.03%), Postives = 322/539 (59.74%), Query Frame = 0

Query: 30  GLKTMPFIIVNEAFERVASLGLTPNMVFYLRDVFGFQIAAASIILSLWSAASNALAIVGA 89
           G K MPFII NE FE++  +G + N+V YL  VF  +   A+ +++++   SN   IV A
Sbjct: 22  GWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITAAKVVNIYGGTSNFGTIVAA 81

Query: 90  VLADSYFGRFRVIILGSFCSLLGMTLLWLTAMIPQLKSSSCSLS-GTLCNSGNPYQLAVL 149
            L DSYFGR++ +        LG   + LTA+I  L  + C+   G++CN  +  Q+  L
Sbjct: 82  FLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQCAKEIGSVCNGPSIGQIMFL 141

Query: 150 FSSLAFISIGAGCIRPCSIAFGADQLTNEEKPNNERVLDSYFNWYYVSIGISSIISLTLI 209
             ++  + IGAG IRPC++ FGADQ   + K   +R ++S+FNWY+ +   + ++SLTLI
Sbjct: 142 AGAMVLLVIGAGGIRPCNLPFGADQFDPKTK-EGKRGIESFFNWYFFTFTFAQMVSLTLI 201

Query: 210 VYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSSLYVKVKPAQNLLIGLLQVLVVAFKNRRL 269
           VY+Q    W IG A+PA LML   +IF  GS LYVKVK + + +  + +V+VVA K RRL
Sbjct: 202 VYVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKASGSPIHSITRVIVVAIKKRRL 261

Query: 270 SLPHSNFDQYYLGHDPK--FLIPTNSMRCLNKACLIKDAESNLNPDGSVSNPWNLCSVDQ 329
                N    Y+  D K   L  T   R L+K+  I+  +  LN DGS  + W LCS+ Q
Sbjct: 262 KPVGPNELYNYIASDFKNSKLGHTEQFRFLDKSA-IQTQDDKLNKDGSPVDAWKLCSMQQ 321

Query: 330 VESLKVFLRIIPMW-STGILMLVCLNQNSFGTIQAKTMNRHI-LGSFEIPAGSITIFMVA 389
           VE +K  +R++P+W S  +  L  + Q ++   Q+   +R +  GSF+IPAGS T+F++ 
Sbjct: 322 VEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSDRRLGPGSFQIPAGSYTVFLML 381

Query: 390 SLSIWITFYDRIMVPLLARY-GHAGGLSPQLRMGIGLLLSSFAMAISAIVAGTRRRLAIE 449
            ++I+I  YDR++VP L +Y G  GG++   R+G GL L   +M +SAIV   RR++A+ 
Sbjct: 382 GMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLFLCITSMMVSAIVEQYRRKVALT 441

Query: 450 E---GLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQLEYFYLRVPKTLSSVAVALYT 509
           +   GL  +  A+  MS +W++PQ  ++G+A+AL  VGQ+E++Y + P+ + S A +LY 
Sbjct: 442 KPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAGVGQMEFYYKQFPENMRSFAGSLYY 501

Query: 510 VEMAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLDYFYWLCAALALINFFGYLI 560
             + +A+ + + L+  V+  T   +  SWL  ++NKG L+YFY+L A +  +N   +L+
Sbjct: 502 CGIGLASYLSTFLLSAVHDTTEGFSGGSWLPEDLNKGRLEYFYFLVAGMMTLNLAYFLL 558

BLAST of Lag0005077 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 3.0e-97
Identity = 211/567 (37.21%), Postives = 332/567 (58.55%), Query Frame = 0

Query: 4   LEDGEKTRIVEISSDGANQASNYRNGGLKTMPFIIVNEAFERVASLGLTPNMVFYLRDVF 63
           +E+  K +I E      +   N   GGL TMPFI  NE  E++A +G   NM+ YL    
Sbjct: 1   MEEQSKNKISE-EEKQLHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQL 60

Query: 64  GFQIAAASIILSLWSAASNALAIVGAVLADSYFGRFRVIILGSFCSLLGMTLLWLTAMIP 123
              +  A+  L+ ++  S+   ++GA +ADS+ GRF  I   S    +GMTLL ++A+IP
Sbjct: 61  HLPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIP 120

Query: 124 QLKSSSCSLSGTLCNSGNPYQLAVLFSSLAFISIGAGCIRPCSIAFGADQLTNEEKPNNE 183
            L+   C     +C   +  QL++L+ +L   ++G+G IRPC +AFGADQ  +E  PN  
Sbjct: 121 TLRPPPCK-GEEVCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQF-DESDPNQT 180

Query: 184 RVLDSYFNWYYVSIGISSIISLTLIVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSSLYV 243
               +YFNWYY  +G + ++++T++V+IQD  GWG+G  +P   M  SV+ F+ G  LY 
Sbjct: 181 TKTWNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYR 240

Query: 244 KVKPAQNLLIGLLQVLVVAFKNRRLSLPHSNFDQYY---------LGHDPKFLIPTNSMR 303
            + PA +    L+QV V AF+ R+L +       Y+         LG     L  T  M 
Sbjct: 241 HLVPAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGK---LTHTKHMS 300

Query: 304 CLNKACLIKDAESNLNPDGSVSNPWNLCSVDQVESLKVFLRIIPMWSTGILMLVC-LNQN 363
            L+KA ++ + E NL P G + N W L +V +VE LK  +R+ P+ ++GIL++     Q 
Sbjct: 301 FLDKAAIVTE-EDNLKP-GQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQG 360

Query: 364 SFGTIQAKTMNRHILGSFEIPAGSITIFMVASLSIWITFYDRIMVPLLARY-GHAGGLSP 423
           +F   QAKTMNRH+  SF+IPAGS+++F   ++   I FYDR+ V +  ++ G   G++ 
Sbjct: 361 TFSLQQAKTMNRHLTNSFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITF 420

Query: 424 QLRMGIGLLLSSFAMAISAIVAGTRRRLAIEEGLEDQPNAVIDMSALWMVPQCAVLGVAE 483
             RMGIG ++S  A  ++  V   R+ +AIE GL D+P+ ++ +S LW++PQ  + GVAE
Sbjct: 421 LHRMGIGFVISIIATLVAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAE 480

Query: 484 ALNSVGQLEYFYLRVPKTLSSVAVALYTVEMAVANLVGSILVQIVNGITGEGNKTSWL-D 543
           A  S+G LE+FY + P+++ S A AL+ + +++ N V ++LV +V+  + + + ++WL D
Sbjct: 481 AFMSIGHLEFFYDQAPESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPD 540

Query: 544 NNINKGHLDYFYWLCAALALINFFGYL 559
           NN+N+G L+YFYWL   L  +N   YL
Sbjct: 541 NNLNRGRLEYFYWLITVLQAVNLVYYL 559

BLAST of Lag0005077 vs. ExPASy TrEMBL
Match: A0A6J1HEG1 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462798 PE=3 SV=1)

HSP 1 Score: 975.3 bits (2520), Expect = 1.1e-280
Identity = 498/585 (85.13%), Postives = 532/585 (90.94%), Query Frame = 0

Query: 1   MEALEDGEKTRIVEISSDGANQASNYRNGGLKTMPFIIVNEAFERVASLGLTPNMVFYLR 60
           ME LEDGEKT I    S+ A QASNY NGGLKTMPFIIVNE FERVASLGLTPNMVFYLR
Sbjct: 1   MEVLEDGEKTGIAASGSEEAEQASNYTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DVFGFQIAAASIILSLWSAASNALAIVGAVLADSYFGRFRVIILGSFCSLLGMTLLWLTA 120
           DVFGF+IAAASIILSLWSAASNALAIVGAVLADSY GRF VIILGS  SLLGM LLWLTA
Sbjct: 61  DVFGFEIAAASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 MIPQLKSSSCSLSGTLCNSGNPYQLAVLFSSLAFISIGAGCIRPCSIAFGADQLTNEEKP 180
           +IPQL+ +SCS SG LC+S NPYQLAVLFSSL FISIGAGCIRPCS+AFGADQLT EEKP
Sbjct: 121 IIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 NNERVLDSYFNWYYVSIGISSIISLTLIVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSS 240
           NNE VLDSYFNWYY SIGI++II+L+LIV+IQDKFGWGIGFAVPA LMLFSVLIFLVGSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIIALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPAQNLLIGLLQVLVVAFKNRRLSLPHSNFDQYYLGHDPKFLIPTNSMRCLNKAC 300
           LYVKVKP Q+LL G L+V+VVAFKNR+LSLP SNFDQYYLG DPK  IPTNSMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCRIPTNSMRYLNKAC 300

Query: 301 LIKDAESNLNPDGSVSNPWNLCSVDQVESLKVFLRIIPMWSTGILMLVCLNQNSFGTIQA 360
           LIKDA++NLNPDGSVSNPWNLCSVDQVESLK FLRIIPMWSTGILMLVCLNQNSFGTIQA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 KTMNRHILGSFEIPAGSITIFMVASLSIWITFYDRIMVPLLARYGHAGGLSPQLRMGIGL 420
           KTMNRHI  SFEIPAGSITIFMV SL  WI FYDRI+VPLLAR+GHAGGLSP+LR+GIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSPKLRIGIGL 420

Query: 421 LLSSFAMAISAIVAGTRRRLAIEEGLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQL 480
           LLS FAMAISA VA  RRR+AIE+G EDQPNAVI+MSALWMVPQC VLGVAEALNSVGQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFYLRVPKTLSSVAVALYTVEMAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLD 540
           EYFY ++PKTLSSVAVALY+ EMAVANLVGSI+VQ+VN ITGEGNKTSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALINFFGYLIFCWAYERQGNESNEMEDFEYRNLPSS 586
           YFYWLCAALALINFFGYL++CWA  RQ  E NEMEDFEYRNLPSS
Sbjct: 541 YFYWLCAALALINFFGYLVYCWALGRQDKEPNEMEDFEYRNLPSS 585

BLAST of Lag0005077 vs. ExPASy TrEMBL
Match: A0A6J1K947 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491783 PE=3 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.3e-278
Identity = 496/585 (84.79%), Postives = 528/585 (90.26%), Query Frame = 0

Query: 1   MEALEDGEKTRIVEISSDGANQASNYRNGGLKTMPFIIVNEAFERVASLGLTPNMVFYLR 60
           ME LEDGEKT I    S+G  QASN+ NGGLKTMPFIIVNE FERVASLGLTPNMVFYLR
Sbjct: 1   MEVLEDGEKTGISASGSEGTEQASNHTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DVFGFQIAAASIILSLWSAASNALAIVGAVLADSYFGRFRVIILGSFCSLLGMTLLWLTA 120
           DVFGFQIA ASIILSLWSAASNALAIVGAVLADSY GRF VIILGS  SLLGM LLWLTA
Sbjct: 61  DVFGFQIADASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 MIPQLKSSSCSLSGTLCNSGNPYQLAVLFSSLAFISIGAGCIRPCSIAFGADQLTNEEKP 180
           +IPQL+ +SCS SG LC+S NPYQLAVLFSSL FISIGAGCIRPCS+AFGADQLT EEKP
Sbjct: 121 IIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 NNERVLDSYFNWYYVSIGISSIISLTLIVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSS 240
           NNE VLDSYFNWYY SIGI++I +L+LIV+IQDKFGWGIGFAVPA LMLFSVLIFLVGSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIFALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPAQNLLIGLLQVLVVAFKNRRLSLPHSNFDQYYLGHDPKFLIPTNSMRCLNKAC 300
           LYVKVKP Q+LL G L+V+VVAFKNR+LSLP SNFDQYYLG DPK  IPTNSMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGCDPKCPIPTNSMRYLNKAC 300

Query: 301 LIKDAESNLNPDGSVSNPWNLCSVDQVESLKVFLRIIPMWSTGILMLVCLNQNSFGTIQA 360
           LIKDA++NLNPDGSVSNPWNLCSVDQVESLK FLRIIPMWSTGILMLVCLNQNSFGTIQA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 KTMNRHILGSFEIPAGSITIFMVASLSIWITFYDRIMVPLLARYGHAGGLSPQLRMGIGL 420
           KTMNRHI  SFEIPAGSITIFMV SL  WI FYDRI+VPLLARYGHAGGLSP+LR+GIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARYGHAGGLSPKLRIGIGL 420

Query: 421 LLSSFAMAISAIVAGTRRRLAIEEGLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQL 480
           LLS FAMAISA VA  RRR+AIE+G EDQPNAVI+MSALWMVPQC VLGVAEALNSVGQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFYLRVPKTLSSVAVALYTVEMAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLD 540
           EYFY ++PK LSSVAVALY+ EMAVANLVGSI+VQ+VN ITGEGNKTSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKMLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALINFFGYLIFCWAYERQGNESNEMEDFEYRNLPSS 586
           YFYWLCAALALINFFGYL+ CWA  RQ  E NEMEDFEYRNLPSS
Sbjct: 541 YFYWLCAALALINFFGYLVCCWALGRQDKEPNEMEDFEYRNLPSS 585

BLAST of Lag0005077 vs. ExPASy TrEMBL
Match: A0A6J1BSW7 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005055 PE=3 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 3.0e-270
Identity = 475/589 (80.65%), Postives = 527/589 (89.47%), Query Frame = 0

Query: 1   MEALEDGEKTRIVEISSDGANQASNYRNGGLKTMPFIIVNEAFERVASLGLTPNMVFYLR 60
           ME +E+ EKT I + SS+G +QASN R GGL+TMPFIIVNE FE++ASLGLTPNMVFYLR
Sbjct: 1   MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR 60

Query: 61  DVFGFQIAAASIILSLWSAASNALAIVGAVLADSYFGRFRVIILGSFCSLLGMTLLWLTA 120
           D +GFQIAAAS ILSLWSAASNALAI GAVLADSY GRFR IILGSF S LGM LLWLTA
Sbjct: 61  DDYGFQIAAASSILSLWSAASNALAIAGAVLADSYVGRFRSIILGSFSSFLGMALLWLTA 120

Query: 121 MIPQLKSSSCSLSGTLCNSGNPYQLAVLFSSLAFISIGAGCIRPCSIAFGADQLTNEEKP 180
           MIPQLK SSCS SG LC+S NPYQLA+LF+SL FISIGAGCIRPCSIAFGADQLTNE+KP
Sbjct: 121 MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP 180

Query: 181 NNERVLDSYFNWYYVSIGISSIISLTLIVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSS 240
           NNE VLD+YFNWYY S GI+S+I+LTLIVYIQD+FGW  GFAVPA LMLFSVLIFLVGSS
Sbjct: 181 NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPAQNLLIGLLQVLVVAFKNRRLSLPHSNFDQYYLGHDPKFLIPTNSMRCLNKAC 300
           LYVKVKP Q+LL GLLQV+VVAFKNRRLSLP+SN+DQYYLG DPKFL+PT+SMRCLNKAC
Sbjct: 241 LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC 300

Query: 301 LIKDAESNLNPDGSVSNPWNLCSVDQVESLKVFLRIIPMWSTGILMLVCLNQNSFGTIQA 360
           LIKDAE++LNPDGS+SNPWNLCSV+ VESLK FLRIIPMWSTGILMLVCLNQNSFGT+QA
Sbjct: 301 LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA 360

Query: 361 KTMNRHILGSFEIPAGSITIFMVASLSIWITFYDRIMVPLLARYGHAGGLSPQLRMGIGL 420
           KTMNRHI GSFEIPAGSI + M+ SLS+W+ FYDRIMVPLL R GHAGGLSP+LRMGIGL
Sbjct: 361 KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL 420

Query: 421 LLSSFAMAISAIVAGTRRRLAIEEGLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQL 480
           LLS FAMAISA+V GTRRR+A EEGLEDQP AVIDMS LW+VPQC VLGVAE  N++GQ+
Sbjct: 421 LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI 480

Query: 481 EYFYLRVPKTLSSVAVALYTVEMAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLD 540
           EYFY ++PKTLSSVAVALYTVEMAVA+LVGS LVQ+VNGITG+GN+TSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD 540

Query: 541 YFYWLCAALALINFFGYLIFCWAY----ERQGNESNEMEDFEYRNLPSS 586
           YFYWLCAALAL+NF GYL+    Y     R+ NESNE++DFEYR LPSS
Sbjct: 541 YFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 589

BLAST of Lag0005077 vs. ExPASy TrEMBL
Match: A0A6J1BQY7 (protein NRT1/ PTR FAMILY 1.2-like OS=Momordica charantia OX=3673 GN=LOC111004925 PE=3 SV=1)

HSP 1 Score: 898.7 bits (2321), Expect = 1.3e-257
Identity = 442/589 (75.04%), Postives = 517/589 (87.78%), Query Frame = 0

Query: 1   MEALEDGEKTRIVEISSDGANQASNYRNGGLKTMPFIIVNEAFERVASLGLTPNMVFYLR 60
           ME +E+ EKT I + S +G +QASN R GGL+TMPFII NE FE+++SLGL+PNM+FY R
Sbjct: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR 60

Query: 61  DVFGFQIAAASIILSLWSAASNALAIVGAVLADSYFGRFRVIILGSFCSLLGMTLLWLTA 120
           D +GFQIAAAS +LSLWSAASNAL+I GAVLADSY GRFRVIILGSFCS LGM LLWLTA
Sbjct: 61  DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA 120

Query: 121 MIPQLKSSSCSLSGTLCNSGNPYQLAVLFSSLAFISIGAGCIRPCSIAFGADQLTNEEKP 180
            IPQLK SSCS SG LC+S N YQLAVLF+SL FISIGAGCI+PCS+AFGADQLTNE+  
Sbjct: 121 TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS 180

Query: 181 NNERVLDSYFNWYYVSIGISSIISLTLIVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSS 240
           +N+ VLD+YFNWYY + GIS++I +T+IVYIQD+FGWG G+ VPA LML S LIFL GSS
Sbjct: 181 SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS 240

Query: 241 LYVKVKPAQNLLIGLLQVLVVAFKNRRLSLPHSNFDQYYLGHDPKFLIPTNSMRCLNKAC 300
           LYVKVKPAQ+L  G+ QV+VVAFKNRRLSLP+SNFDQYYLGHDPK L+PT+SMRCLNKAC
Sbjct: 241 LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC 300

Query: 301 LIKDAESNLNPDGSVSNPWNLCSVDQVESLKVFLRIIPMWSTGILMLVCLNQNSFGTIQA 360
           LIKDAE++LNPDGSVSNPWNLCSVDQVESLK F+RIIP+WSTGI+M+VC+NQN+ G +QA
Sbjct: 301 LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA 360

Query: 361 KTMNRHILGSFEIPAGSITIFMVASLSIWITFYDRIMVPLLARYGHAGGLSPQLRMGIGL 420
           K MNRHI GSFE PAGSI +FM+ SLS+W+ FYDRI++PLL R GHAG LSP+LR+GIGL
Sbjct: 361 KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL 420

Query: 421 LLSSFAMAISAIVAGTRRRLAIEEGLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQL 480
           LLS FAMAI+A+V  TRRR+AIEEGLEDQPNAV+DMSALW+VPQC VLGVAE  N+VGQ+
Sbjct: 421 LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI 480

Query: 481 EYFYLRVPKTLSSVAVALYTVEMAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLD 540
           EYF+ ++ K  SS+AVAL+TVEM+VANLVGSILVQIVNGITG+GN+TSWLDNN+NKGHLD
Sbjct: 481 EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD 540

Query: 541 YFYWLCAALALINFFGYLIFCWAY----ERQGNESNEMEDFEYRNLPSS 586
           YFYWLCAALAL+N  GYL++  AY    + QGNES+E++DFEYRNLPSS
Sbjct: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589

BLAST of Lag0005077 vs. ExPASy TrEMBL
Match: A0A6J1HGQ1 (protein NRT1/ PTR FAMILY 1.2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462798 PE=3 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 5.8e-229
Identity = 402/473 (84.99%), Postives = 434/473 (91.75%), Query Frame = 0

Query: 113 MTLLWLTAMIPQLKSSSCSLSGTLCNSGNPYQLAVLFSSLAFISIGAGCIRPCSIAFGAD 172
           M LLWLTA+IPQL+ +SCS SG LC+S NPYQLAVLFSSL FISIGAGCIRPCS+AFGAD
Sbjct: 1   MILLWLTAIIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGAD 60

Query: 173 QLTNEEKPNNERVLDSYFNWYYVSIGISSIISLTLIVYIQDKFGWGIGFAVPAGLMLFSV 232
           QLT EEKPNNE VLDSYFNWYY SIGI++II+L+LIV+IQDKFGWGIGFAVPA LMLFSV
Sbjct: 61  QLTKEEKPNNESVLDSYFNWYYASIGIATIIALSLIVFIQDKFGWGIGFAVPAVLMLFSV 120

Query: 233 LIFLVGSSLYVKVKPAQNLLIGLLQVLVVAFKNRRLSLPHSNFDQYYLGHDPKFLIPTNS 292
           LIFLVGSSLYVKVKP Q+LL G L+V+VVAFKNR+LSLP SNFDQYYLG DPK  IPTNS
Sbjct: 121 LIFLVGSSLYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCRIPTNS 180

Query: 293 MRCLNKACLIKDAESNLNPDGSVSNPWNLCSVDQVESLKVFLRIIPMWSTGILMLVCLNQ 352
           MR LNKACLIKDA++NLNPDGSVSNPWNLCSVDQVESLK FLRIIPMWSTGILMLVCLNQ
Sbjct: 181 MRYLNKACLIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQ 240

Query: 353 NSFGTIQAKTMNRHILGSFEIPAGSITIFMVASLSIWITFYDRIMVPLLARYGHAGGLSP 412
           NSFGTIQAKTMNRHI  SFEIPAGSITIFMV SL  WI FYDRI+VPLLAR+GHAGGLSP
Sbjct: 241 NSFGTIQAKTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSP 300

Query: 413 QLRMGIGLLLSSFAMAISAIVAGTRRRLAIEEGLEDQPNAVIDMSALWMVPQCAVLGVAE 472
           +LR+GIGLLLS FAMAISA VA  RRR+AIE+G EDQPNAVI+MSALWMVPQC VLGVAE
Sbjct: 301 KLRIGIGLLLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAE 360

Query: 473 ALNSVGQLEYFYLRVPKTLSSVAVALYTVEMAVANLVGSILVQIVNGITGEGNKTSWLDN 532
           ALNSVGQ+EYFY ++PKTLSSVAVALY+ EMAVANLVGSI+VQ+VN ITGEGNKTSWLDN
Sbjct: 361 ALNSVGQVEYFYSQMPKTLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDN 420

Query: 533 NINKGHLDYFYWLCAALALINFFGYLIFCWAYERQGNESNEMEDFEYRNLPSS 586
           N+NKGHLDYFYWLCAALALINFFGYL++CWA  RQ  E NEMEDFEYRNLPSS
Sbjct: 421 NVNKGHLDYFYWLCAALALINFFGYLVYCWALGRQDKEPNEMEDFEYRNLPSS 473

BLAST of Lag0005077 vs. TAIR 10
Match: AT1G52190.1 (Major facilitator superfamily protein )

HSP 1 Score: 529.6 bits (1363), Expect = 3.1e-150
Identity = 268/544 (49.26%), Postives = 376/544 (69.12%), Query Frame = 0

Query: 29  GGLKTMPFIIVNEAFERVASLGLTPNMVFYLRDVFGFQIAAASIILSLWSAASNALAIVG 88
           GG+ TMPFII NEAFE+VAS GL PNM+ YL   + F +A  + +L +WSAASN   ++G
Sbjct: 23  GGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAASNFTPLLG 82

Query: 89  AVLADSYFGRFRVIILGSFCSLLGMTLLWLTAMIPQLKSSSC--SLSGTLCNSGNPYQLA 148
           A L+DSY GRF  I + S  S LGM LLWLTAM+PQ+K S C  + +G+ C S    QLA
Sbjct: 83  AFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCGSSTASQLA 142

Query: 149 VLFSSLAFISIGAGCIRPCSIAFGADQLTNEEKPNNERVLDSYFNWYYVSIGISSIISLT 208
           +L+S+ A ISIG+G IRPCS+AFGADQL N+E P NERVL+S+F WYY S  ++ +I+ T
Sbjct: 143 LLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSAVAVLIAFT 202

Query: 209 LIVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSSLYVKVKPAQNLLIGLLQVLVVAFKNR 268
            IVYIQ+  GW IGF VPA LML + L+F++ S LYV     ++L  GL Q +V A+K R
Sbjct: 203 GIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQAIVAAYKKR 262

Query: 269 RLSLP--HSNFDQYYLGHDPKFLIPTNSMRCLNKACLIKDAESNLNPDGSVSNPWNLCSV 328
           +LSLP  H +FD YY   D +   P+  +R LNKACLI + E  +  DG   NPW LC+ 
Sbjct: 263 KLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFALNPWRLCTT 322

Query: 329 DQVESLKVFLRIIPMWSTGILMLVCLNQNSFGTIQAKTMNRHIL---GSFEIPAGSITIF 388
           D+VE LK  +++IP+WSTGI+M +  +Q+SF  +QA +M+R +     SF++PAGS  +F
Sbjct: 323 DKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQVPAGSFGMF 382

Query: 389 MVASLSIWITFYDRIMVPLLARY-GHAGGLSPQLRMGIGLLLSSFAMAISAIVAGTRRRL 448
            + +L++W+  YDR ++PL ++  G    LS +LRMG+GL +S  AMAISA+V   RR+ 
Sbjct: 383 TIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAMVESFRRKK 442

Query: 449 AIEEGLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQLEYFYLRVPKTLSSVAVALYT 508
           AI +G  +  NAV+D+SA+W+VPQ  + G+AEAL ++GQ E+FY   PK++SS+A +L+ 
Sbjct: 443 AISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMSSIAASLFG 502

Query: 509 VEMAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLDYFYWLCAALALINFFGYLIF 565
           + MAVA+L+ S+++  VN +T    K SW+ +NINKGH +Y+YW+ A ++ IN   Y+I 
Sbjct: 503 LGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFINVIYYVIC 562

BLAST of Lag0005077 vs. TAIR 10
Match: AT3G16180.1 (Major facilitator superfamily protein )

HSP 1 Score: 507.7 bits (1306), Expect = 1.3e-143
Identity = 255/542 (47.05%), Postives = 370/542 (68.27%), Query Frame = 0

Query: 29  GGLKTMPFIIVNEAFERVASLGLTPNMVFYLRDVFGFQIAAASIILSLWSAASNALAIVG 88
           GGL TMPFII NE FE+VAS GL  NM+ YL   +   +     +L +W AA+N + +VG
Sbjct: 25  GGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVKGQTVLFMWVAATNFMPLVG 84

Query: 89  AVLADSYFGRFRVIILGSFCSLLGMTLLWLTAMIPQLKSSSC-SLSGTLCNSGNPYQLAV 148
           A L+DSY GRF  I++ S  SLLGM +LWLTAM+PQ+K S C + +GT C+S    QLA+
Sbjct: 85  AFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLAL 144

Query: 149 LFSSLAFISIGAGCIRPCSIAFGADQLTNEEKPNNERVLDSYFNWYYVSIGISSIISLTL 208
           L+++ A ISIG+G IRPCS+AFGADQL N+E P NERVL+S+F WYY S  ++ +I+ T+
Sbjct: 145 LYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFTV 204

Query: 209 IVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSSLYVKVKPAQNLLIGLLQVLVVAFKNRR 268
           IVYIQD  GW IGF +PA LML +  +F+  S LYVK   +++L  GL QV+  A+  R 
Sbjct: 205 IVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKRN 264

Query: 269 LSLP--HSNFDQYYLGHDPKFLIPTNSMRCLNKACLIKDAESNLNPDGSVSNPWNLCSVD 328
           L+LP  H + D YY   D +   P++ +R LNKAC I + + +L  DG   N W LC+ D
Sbjct: 265 LTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAISNRDEDLGSDGLALNQWRLCTTD 324

Query: 329 QVESLKVFLRIIPMWSTGILMLVCLNQNSFGTIQAKTMNRHIL--GSFEIPAGSITIFMV 388
           QVE LK  +++IP+WSTGI+M + ++QNSF  +QAK+M+R +    +F+IPAGS  +F +
Sbjct: 325 QVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMFTI 384

Query: 389 ASLSIWITFYDRIMVPLLARY-GHAGGLSPQLRMGIGLLLSSFAMAISAIVAGTRRRLAI 448
            +L  W+  YDR ++PL ++  G    ++ ++RMG+GL +S  AMA+SA V   RR+ AI
Sbjct: 385 IALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKTAI 444

Query: 449 EEGLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQLEYFYLRVPKTLSSVAVALYTVE 508
            +GL +  N+ + +SA+W+VPQ  + G+AEAL  +GQ E+FY   PK++SS+A +L+ + 
Sbjct: 445 SQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFGLG 504

Query: 509 MAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLDYFYWLCAALALINFFGYLIFCW 565
           MAVAN++ S+++  V   + +GN  SW+++NINKGH DY+YW+ A L+ +N   Y++  W
Sbjct: 505 MAVANILASVILNAVKNSSKQGN-VSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVCSW 564

BLAST of Lag0005077 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 386.7 bits (992), Expect = 3.2e-107
Identity = 213/562 (37.90%), Postives = 336/562 (59.79%), Query Frame = 0

Query: 29  GGLKTMPFIIVNEAFERVASLGLTPNMVFYLRDVFGFQIAAASIILSLWSAASNALAIVG 88
           GG + + FI+ NE  ER+ S+GL  N + YL  VF  +   A+ ++++WS  +N   +VG
Sbjct: 53  GGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVG 112

Query: 89  AVLADSYFGRFRVIILGSFCSLLGMTLLWLTAMIPQLKSSSCSLSGTL-CNSGNPYQLAV 148
           A ++D+Y GRF+ I   SF +LLG+  + LTA  PQL  +SC+    L C   N  Q+ V
Sbjct: 113 AYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGV 172

Query: 149 LFSSLAFISIGAGCIRPCSIAFGADQLTNEEKPNNERVLDSYFNWYYVSIGISSIISLTL 208
           L   L F+S+G+G IRPCSI FG DQ  ++      + + S+FNWYY++  +  II+ T+
Sbjct: 173 LLLGLCFLSVGSGGIRPCSIPFGVDQF-DQRTEEGVKGVASFFNWYYMTFTVVLIITQTV 232

Query: 209 IVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSSLYVKVKPAQNLLIGLLQVLVVAFKNRR 268
           +VYIQD+  W IGF++P GLM  +V++F  G   YV VKP  ++  G+ QV+V A K R+
Sbjct: 233 VVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRK 292

Query: 269 LSLPHSNFDQYYLGHDPKF-------LIPTNSMRCLNKACLIKDAESNLNPDGSVSNPWN 328
           L LP  + D     +DP         L  +N  RCL+KA ++   E +L P+G  ++ W 
Sbjct: 293 LKLPAED-DGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADKWR 352

Query: 329 LCSVDQVESLKVFLRIIPMWSTGILMLVCL-NQNSFGTIQAKTMNRHILGSFEIPAGSIT 388
           LCSV +VE +K  +RI+P+WS GI+ L  +  Q +F   QA  M+R++   FEIPAGS++
Sbjct: 353 LCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLS 412

Query: 389 IFMVASLSIWITFYDRIMVPLLARY-GHAGGLSPQLRMGIGLLLSSFAMAISAIVAGTRR 448
           +  + ++ I++ FYDR+ VP + R  GH  G++   R+G G++ + F+M ++ IV   RR
Sbjct: 413 VISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRR 472

Query: 449 RLAIEEGLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQLEYFYLRVPKTLSSVAVAL 508
             +I  G    P  +  MS  W+ PQ  ++G+ EA N +GQ+E+F  + P+ + S+A +L
Sbjct: 473 IRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSL 532

Query: 509 YTVEMAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLDYFYWLCAALALINFFGYL 568
           +++  A ++ + S LV +V+  +G  ++  WL+ N+N G LDYFY+L A L ++N    L
Sbjct: 533 FSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVN----L 592

Query: 569 IFCWAYER--QGNESNEMEDFE 579
           ++ W   R  +      +EDFE
Sbjct: 593 VYFWYCARGYRYKVGLPIEDFE 603

BLAST of Lag0005077 vs. TAIR 10
Match: AT1G18880.1 (Major facilitator superfamily protein )

HSP 1 Score: 360.5 bits (924), Expect = 2.5e-99
Identity = 205/539 (38.03%), Postives = 322/539 (59.74%), Query Frame = 0

Query: 30  GLKTMPFIIVNEAFERVASLGLTPNMVFYLRDVFGFQIAAASIILSLWSAASNALAIVGA 89
           G K MPFII NE FE++  +G + N+V YL  VF  +   A+ +++++   SN   IV A
Sbjct: 22  GWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITAAKVVNIYGGTSNFGTIVAA 81

Query: 90  VLADSYFGRFRVIILGSFCSLLGMTLLWLTAMIPQLKSSSCSLS-GTLCNSGNPYQLAVL 149
            L DSYFGR++ +        LG   + LTA+I  L  + C+   G++CN  +  Q+  L
Sbjct: 82  FLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQCAKEIGSVCNGPSIGQIMFL 141

Query: 150 FSSLAFISIGAGCIRPCSIAFGADQLTNEEKPNNERVLDSYFNWYYVSIGISSIISLTLI 209
             ++  + IGAG IRPC++ FGADQ   + K   +R ++S+FNWY+ +   + ++SLTLI
Sbjct: 142 AGAMVLLVIGAGGIRPCNLPFGADQFDPKTK-EGKRGIESFFNWYFFTFTFAQMVSLTLI 201

Query: 210 VYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSSLYVKVKPAQNLLIGLLQVLVVAFKNRRL 269
           VY+Q    W IG A+PA LML   +IF  GS LYVKVK + + +  + +V+VVA K RRL
Sbjct: 202 VYVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKASGSPIHSITRVIVVAIKKRRL 261

Query: 270 SLPHSNFDQYYLGHDPK--FLIPTNSMRCLNKACLIKDAESNLNPDGSVSNPWNLCSVDQ 329
                N    Y+  D K   L  T   R L+K+  I+  +  LN DGS  + W LCS+ Q
Sbjct: 262 KPVGPNELYNYIASDFKNSKLGHTEQFRFLDKSA-IQTQDDKLNKDGSPVDAWKLCSMQQ 321

Query: 330 VESLKVFLRIIPMW-STGILMLVCLNQNSFGTIQAKTMNRHI-LGSFEIPAGSITIFMVA 389
           VE +K  +R++P+W S  +  L  + Q ++   Q+   +R +  GSF+IPAGS T+F++ 
Sbjct: 322 VEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSDRRLGPGSFQIPAGSYTVFLML 381

Query: 390 SLSIWITFYDRIMVPLLARY-GHAGGLSPQLRMGIGLLLSSFAMAISAIVAGTRRRLAIE 449
            ++I+I  YDR++VP L +Y G  GG++   R+G GL L   +M +SAIV   RR++A+ 
Sbjct: 382 GMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLFLCITSMMVSAIVEQYRRKVALT 441

Query: 450 E---GLEDQPNAVIDMSALWMVPQCAVLGVAEALNSVGQLEYFYLRVPKTLSSVAVALYT 509
           +   GL  +  A+  MS +W++PQ  ++G+A+AL  VGQ+E++Y + P+ + S A +LY 
Sbjct: 442 KPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAGVGQMEFYYKQFPENMRSFAGSLYY 501

Query: 510 VEMAVANLVGSILVQIVNGITGEGNKTSWLDNNINKGHLDYFYWLCAALALINFFGYLI 560
             + +A+ + + L+  V+  T   +  SWL  ++NKG L+YFY+L A +  +N   +L+
Sbjct: 502 CGIGLASYLSTFLLSAVHDTTEGFSGGSWLPEDLNKGRLEYFYFLVAGMMTLNLAYFLL 558

BLAST of Lag0005077 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 357.5 bits (916), Expect = 2.1e-98
Identity = 211/567 (37.21%), Postives = 332/567 (58.55%), Query Frame = 0

Query: 4   LEDGEKTRIVEISSDGANQASNYRNGGLKTMPFIIVNEAFERVASLGLTPNMVFYLRDVF 63
           +E+  K +I E      +   N   GGL TMPFI  NE  E++A +G   NM+ YL    
Sbjct: 1   MEEQSKNKISE-EEKQLHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQL 60

Query: 64  GFQIAAASIILSLWSAASNALAIVGAVLADSYFGRFRVIILGSFCSLLGMTLLWLTAMIP 123
              +  A+  L+ ++  S+   ++GA +ADS+ GRF  I   S    +GMTLL ++A+IP
Sbjct: 61  HLPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIP 120

Query: 124 QLKSSSCSLSGTLCNSGNPYQLAVLFSSLAFISIGAGCIRPCSIAFGADQLTNEEKPNNE 183
            L+   C     +C   +  QL++L+ +L   ++G+G IRPC +AFGADQ  +E  PN  
Sbjct: 121 TLRPPPCK-GEEVCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQF-DESDPNQT 180

Query: 184 RVLDSYFNWYYVSIGISSIISLTLIVYIQDKFGWGIGFAVPAGLMLFSVLIFLVGSSLYV 243
               +YFNWYY  +G + ++++T++V+IQD  GWG+G  +P   M  SV+ F+ G  LY 
Sbjct: 181 TKTWNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYR 240

Query: 244 KVKPAQNLLIGLLQVLVVAFKNRRLSLPHSNFDQYY---------LGHDPKFLIPTNSMR 303
            + PA +    L+QV V AF+ R+L +       Y+         LG     L  T  M 
Sbjct: 241 HLVPAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGK---LTHTKHMS 300

Query: 304 CLNKACLIKDAESNLNPDGSVSNPWNLCSVDQVESLKVFLRIIPMWSTGILMLVC-LNQN 363
            L+KA ++ + E NL P G + N W L +V +VE LK  +R+ P+ ++GIL++     Q 
Sbjct: 301 FLDKAAIVTE-EDNLKP-GQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQG 360

Query: 364 SFGTIQAKTMNRHILGSFEIPAGSITIFMVASLSIWITFYDRIMVPLLARY-GHAGGLSP 423
           +F   QAKTMNRH+  SF+IPAGS+++F   ++   I FYDR+ V +  ++ G   G++ 
Sbjct: 361 TFSLQQAKTMNRHLTNSFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITF 420

Query: 424 QLRMGIGLLLSSFAMAISAIVAGTRRRLAIEEGLEDQPNAVIDMSALWMVPQCAVLGVAE 483
             RMGIG ++S  A  ++  V   R+ +AIE GL D+P+ ++ +S LW++PQ  + GVAE
Sbjct: 421 LHRMGIGFVISIIATLVAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAE 480

Query: 484 ALNSVGQLEYFYLRVPKTLSSVAVALYTVEMAVANLVGSILVQIVNGITGEGNKTSWL-D 543
           A  S+G LE+FY + P+++ S A AL+ + +++ N V ++LV +V+  + + + ++WL D
Sbjct: 481 AFMSIGHLEFFYDQAPESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPD 540

Query: 544 NNINKGHLDYFYWLCAALALINFFGYL 559
           NN+N+G L+YFYWL   L  +N   YL
Sbjct: 541 NNLNRGRLEYFYWLITVLQAVNLVYYL 559

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022962308.12.3e-28085.13protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Cucurbita moschata][more]
KAG6598782.11.5e-27984.79Protein NRT1/ PTR FAMILY 1.2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023545603.15.7e-27984.62protein NRT1/ PTR FAMILY 1.2-like [Cucurbita pepo subsp. pepo][more]
XP_022996584.14.8e-27884.79protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Cucurbita maxima][more]
XP_022132097.16.3e-27080.65protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9M8174.4e-14949.26Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1[more]
Q8LPL21.8e-14247.05Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2[more]
Q8RX774.6e-10637.90Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV... [more]
Q9M9V73.5e-9838.03Protein NRT1/ PTR FAMILY 2.9 OS=Arabidopsis thaliana OX=3702 GN=NPF2.9 PE=1 SV=1[more]
Q9SX203.0e-9737.21Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HEG11.1e-28085.13protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1K9472.3e-27884.79protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1BSW73.0e-27080.65protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1BQY71.3e-25775.04protein NRT1/ PTR FAMILY 1.2-like OS=Momordica charantia OX=3673 GN=LOC111004925... [more]
A0A6J1HGQ15.8e-22984.99protein NRT1/ PTR FAMILY 1.2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT1G52190.13.1e-15049.26Major facilitator superfamily protein [more]
AT3G16180.11.3e-14347.05Major facilitator superfamily protein [more]
AT1G69870.13.2e-10737.90nitrate transporter 1.7 [more]
AT1G18880.12.5e-9938.03Major facilitator superfamily protein [more]
AT1G68570.12.1e-9837.21Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 5..574
e-value: 3.0E-159
score: 532.6
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 32..572
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 99..530
e-value: 3.8E-81
score: 272.9
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 25..567
NoneNo IPR availablePANTHERPTHR11654:SF529SUBFAMILY NOT NAMEDcoord: 25..567
NoneNo IPR availableCDDcd17416MFS_NPF1_2coord: 34..564
e-value: 2.8973E-171
score: 491.396

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0005077.1Lag0005077.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity