Lag0004961 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0004961
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Chloroquine-resistance transporter-like
Location	chr6: 9019485 .. 9020113 (+)
RNA-Seq Expression	Lag0004961
Synteny	Lag0004961

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGATGCAGAAGATTGTGTTATCTTGAGATAAGCATATCCAAATCTGAGAATGGTCCATTAATTATCAGCCCTGGTCTCTTCTCTCTTCCATTCCACGGGACGAGTACTAGCTATTCCTTGAATCAACGTGTTAGCAACTCCCATTGGCTTGAATCGATTCCTCTGAATGATGAATTAACTTCTTTGACTCTTAATGAAATTCGGGTTTTGCATTCGAAAAGAAAAACGCCAAGCCAAAACTAATGGACATATGATCTATTGAATGCAGGTTGTGAAGGGGCTCCATTGCTTCCACTGCTTTACGTGATTACAAATTTGTCCTTCAACATAACTTTGCTCAGCTTGGTGAAGAAATCTTCGGCAGTCGTTGCTTCTCTTGTTGTGATGCTTTCAGGTTCTTTTCTTCTCTGTAGAATATATGAACTAATATTGGATAATGTTATTTCATATGCATGAGCTAAAGATTGGTCTTGTTGCCATTTGCAGTGCCAATTTCAATTTATATTCTATCACTTCCATTGCCATATCTACCTGAAGGCGCAAGCTTAAGCCCCCTCTTTCTCACGGGGAGTTTAATGCTTGTGTTGGGTCTTGCTTTTTACTACATTCCTCAGCGTTCAAAGTAA

mRNA sequence

ATGAGATGCAGAAGATTGTGTTATCTTGAGATAAGCATATCCAAATCTGAGAATGGTCCATTAATTATCAGCCCTGGTCTCTTCTCTCTTCCATTCCACGGGACGAGTTGTGAAGGGGCTCCATTGCTTCCACTGCTTTACGTGATTACAAATTTGTCCTTCAACATAACTTTGCTCAGCTTGGTGAAGAAATCTTCGGCAGTCGTTGCTTCTCTTGTTGTGATGCTTTCAGTGCCAATTTCAATTTATATTCTATCACTTCCATTGCCATATCTACCTGAAGGCGCAAGCTTAAGCCCCCTCTTTCTCACGGGGAGTTTAATGCTTGTGTTGGGTCTTGCTTTTTACTACATTCCTCAGCGTTCAAAGTAA

Coding sequence (CDS)

ATGAGATGCAGAAGATTGTGTTATCTTGAGATAAGCATATCCAAATCTGAGAATGGTCCATTAATTATCAGCCCTGGTCTCTTCTCTCTTCCATTCCACGGGACGAGTTGTGAAGGGGCTCCATTGCTTCCACTGCTTTACGTGATTACAAATTTGTCCTTCAACATAACTTTGCTCAGCTTGGTGAAGAAATCTTCGGCAGTCGTTGCTTCTCTTGTTGTGATGCTTTCAGTGCCAATTTCAATTTATATTCTATCACTTCCATTGCCATATCTACCTGAAGGCGCAAGCTTAAGCCCCCTCTTTCTCACGGGGAGTTTAATGCTTGTGTTGGGTCTTGCTTTTTACTACATTCCTCAGCGTTCAAAGTAA

Protein sequence

MRCRRLCYLEISISKSENGPLIISPGLFSLPFHGTSCEGAPLLPLLYVITNLSFNITLLSLVKKSSAVVASLVVMLSVPISIYILSLPLPYLPEGASLSPLFLTGSLMLVLGLAFYYIPQRSK

Homology

[Close]

BLAST of Lag0004961 vs. NCBI nr
Match: KAG7020259.1 (Protein CLT2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 151.0 bits (380), Expect = 6.8e-33
Identity = 80/91 (87.91%), Postives = 86/91 (94.51%), Query Frame = 0


Query: 33  HGTSCEGAPLLPLLYVITNLSFNITLLSLVKKSSAVVASLVVMLSVPISIYILSLPLPYL 92
           HG  C GAPLLPLLYVITNLSFNITLLSLVKKSSAV++SLVVMLSVP+SIYILSLPLPYL
Sbjct: 321 HGAGCGGAPLLPLLYVITNLSFNITLLSLVKKSSAVISSLVVMLSVPVSIYILSLPLPYL 380

Query: 93  PEGASLSPLFLTGSLMLVLGLAFYYIPQRSK 124
           P+GA+LSPLFLTGSLMLVLGLA YY PQR+K
Sbjct: 381 PQGATLSPLFLTGSLMLVLGLALYYSPQRTK 411

[Close]

BLAST of Lag0004961 vs. NCBI nr
Match: XP_038885312.1 (protein CLT2, chloroplastic [Benincasa hispida])

HSP 1 Score: 149.1 bits (375), Expect = 2.6e-32
Identity = 81/86 (94.19%), Postives = 85/86 (98.84%), Query Frame = 0


Query: 37  CEGAPLLPLLYVITNLSFNITLLSLVKKSSAVVASLVVMLSVPISIYILSLPLPYLPEGA 96
           CEGAPLLPLLYVITNLSFNIT+LS+VKKSSAVVASLVVMLSVP+SIYILSLPLPYLP+GA
Sbjct: 354 CEGAPLLPLLYVITNLSFNITVLSVVKKSSAVVASLVVMLSVPVSIYILSLPLPYLPQGA 413

Query: 97  SLSPLFLTGSLMLVLGLAFYYIPQRS 123
           SLSPLFLTGSLMLVLGLAFYYIPQ S
Sbjct: 414 SLSPLFLTGSLMLVLGLAFYYIPQPS 439

[Close]

BLAST of Lag0004961 vs. NCBI nr
Match: XP_022132091.1 (protein CLT2, chloroplastic-like [Momordica charantia])

HSP 1 Score: 149.1 bits (375), Expect = 2.6e-32
Identity = 81/87 (93.10%), Postives = 85/87 (97.70%), Query Frame = 0


Query: 37  CEGAPLLPLLYVITNLSFNITLLSLVKKSSAVVASLVVMLSVPISIYILSLPLPYLPEGA 96
           C+GAPLLPLLYVITNL+FNITLLSLVKK SAVVASL+VMLSVPISIYILSLPLPYLPEGA
Sbjct: 342 CKGAPLLPLLYVITNLAFNITLLSLVKKFSAVVASLLVMLSVPISIYILSLPLPYLPEGA 401

Query: 97  SLSPLFLTGSLMLVLGLAFYYIPQRSK 124
           SLSPLFLTGSL+LVLGL FYYIPQRSK
Sbjct: 402 SLSPLFLTGSLLLVLGLVFYYIPQRSK 428

[Close]

BLAST of Lag0004961 vs. NCBI nr
Match: XP_023001859.1 (protein CLT2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 147.5 bits (371), Expect = 7.5e-32
Identity = 79/87 (90.80%), Postives = 85/87 (97.70%), Query Frame = 0


Query: 37  CEGAPLLPLLYVITNLSFNITLLSLVKKSSAVVASLVVMLSVPISIYILSLPLPYLPEGA 96
           C GAPLLPLLYVITNLSFNITLLSLVKKSSAV++SLVVMLSVP+SIYILSLPLPYLP+GA
Sbjct: 340 CGGAPLLPLLYVITNLSFNITLLSLVKKSSAVISSLVVMLSVPVSIYILSLPLPYLPQGA 399

Query: 97  SLSPLFLTGSLMLVLGLAFYYIPQRSK 124
           +LSPLFLTGSLMLVLGLA YYIPQR+K
Sbjct: 400 TLSPLFLTGSLMLVLGLALYYIPQRAK 426

[Close]

BLAST of Lag0004961 vs. NCBI nr
Match: XP_023537000.1 (protein CLT2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 145.2 bits (365), Expect = 3.7e-31
Identity = 78/87 (89.66%), Postives = 84/87 (96.55%), Query Frame = 0


Query: 37  CEGAPLLPLLYVITNLSFNITLLSLVKKSSAVVASLVVMLSVPISIYILSLPLPYLPEGA 96
           C GAPLLPLLYVITNLSFNITLLSLVKKSSAV++SLVVMLSVP+SIYILSLPLPYLP+GA
Sbjct: 340 CGGAPLLPLLYVITNLSFNITLLSLVKKSSAVISSLVVMLSVPVSIYILSLPLPYLPQGA 399

Query: 97  SLSPLFLTGSLMLVLGLAFYYIPQRSK 124
           +LSPLFLTGSLMLVLGLA YY PQR+K
Sbjct: 400 TLSPLFLTGSLMLVLGLALYYSPQRTK 426

[Close]

BLAST of Lag0004961 vs. ExPASy Swiss-Prot
Match: A1L4X0 (Protein CLT2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CLT2 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 3.6e-21
Identity = 54/85 (63.53%), Postives = 70/85 (82.35%), Query Frame = 0


Query: 35  TSCEGAPLLPLLYVITNLSFNITLLSLVKKSSAVVASLVVMLSVPISIYILSLPLPYLPE 94
           + C+GAP+LPLLY+ TNL+FNI+LL LVK SSA+V+SL +MLSVP+++YI+S PLPYLP 
Sbjct: 336 SGCDGAPILPLLYISTNLAFNISLLHLVKISSAIVSSLTMMLSVPLAVYIMSKPLPYLPG 395

Query: 95  GASLSPLFLTGSLMLVLGLAFYYIP 120
           G+SLS  F  G ++LVLGL  Y IP
Sbjct: 396 GSSLSSNFTMGCIVLVLGLLLYNIP 420

[Close]

BLAST of Lag0004961 vs. ExPASy Swiss-Prot
Match: Q058P6 (Protein CLT1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CLT1 PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 8.6e-15
Identity = 42/82 (51.22%), Postives = 64/82 (78.05%), Query Frame = 0


Query: 35  TSCEGAPLLPLLYVITNLSFNITLLSLVKKSSAVVASLVVMLSVPISIYILSLPLPYLPE 94
           T CEGAPLLP+++V+ N+++NI+LL L+K SSAVV+SL   +SVPI++Y  +LPLPYL  
Sbjct: 347 TGCEGAPLLPVMFVMMNMAYNISLLRLIKISSAVVSSLASTVSVPIAVYCFTLPLPYLGV 406

Query: 95  GASLSPLFLTGSLMLVLGLAFY 117
            ++L   F+ G+++LV+G+  Y
Sbjct: 407 ASTLPRGFVAGTIILVVGMLLY 428

[Close]

BLAST of Lag0004961 vs. ExPASy Swiss-Prot
Match: Q8RWL5 (Protein CLT3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CLT3 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.2e-13
Identity = 41/80 (51.25%), Postives = 60/80 (75.00%), Query Frame = 0


Query: 37  CEGAPLLPLLYVITNLSFNITLLSLVKKSSAVVASLVVMLSVPISIYILSLPLPYLPEGA 96
           C+GAP LPLL+VI N+ +NI LL L+K SSAVV+ L   +SVPI++++ ++PLPYL   +
Sbjct: 351 CDGAPFLPLLFVIMNIGYNIALLRLLKISSAVVSCLASTVSVPIAVFLFTMPLPYLGVAS 410

Query: 97  SLSPLFLTGSLMLVLGLAFY 117
           SL   F+ G+++LVLG+  Y
Sbjct: 411 SLPKGFMGGTIILVLGMILY 430

The following BLAST results are available for this feature:

BLAST of Lag0004961 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7020259.1	6.8e-33	87.91	Protein CLT2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_038885312.1	2.6e-32	94.19	protein CLT2, chloroplastic [Benincasa hispida]	[more]
XP_022132091.1	2.6e-32	93.10	protein CLT2, chloroplastic-like [Momordica charantia]	[more]
XP_023001859.1	7.5e-32	90.80	protein CLT2, chloroplastic [Cucurbita maxima]	[more]
XP_023537000.1	3.7e-31	89.66	protein CLT2, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0004961 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A1L4X0	3.6e-21	63.53	Protein CLT2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CLT2 PE=2 SV=1	[more]
Q058P6	8.6e-15	51.22	Protein CLT1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CLT1 PE=2 SV=1	[more]
Q8RWL5	1.2e-13	51.25	Protein CLT3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CLT3 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013936	Chloroquine-resistance transporter-like	PFAM	PF08627	CRT-like	coord: 35..116 e-value: 7.1E-7 score: 28.5
IPR013936	Chloroquine-resistance transporter-like	PANTHER	PTHR31326	PROTEIN CLT2, CHLOROPLASTIC	coord: 35..122
None	No IPR available	PANTHER	PTHR31326:SF1	PROTEIN CLT2, CHLOROPLASTIC	coord: 35..122

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0004961.1	Lag0004961.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane