Lag0004756 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0004756 vs. NCBI nr
Match: XP_023546080.1 (probable WRKY transcription factor 57 [Cucurbita pepo subsp. pepo] >XP_023546081.1 probable WRKY transcription factor 57 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 410.2 bits (1053), Expect = 1.5e-110
Identity = 230/308 (74.68%), Postives = 242/308 (78.57%), Query Frame = 0

Query: 2   DPDPPPDFSPADSSTDWTNSLASASADDAAYFFSADRDSTILTEFGWNFHSHDLQPPRFL 61
           +PD PP FS ADSST WTN+L SA   D+ YFF ADRDS +LTEFGWNF SHD QP RFL
Sbjct: 6   NPDSPPSFSTADSSTVWTNALPSA---DSFYFFPADRDSAVLTEFGWNFLSHDAQPTRFL 65

Query: 62  HSDKADFPGTSAPPIDDGVGGGLQSPDAPATAGSLNDAVASNPSLSSSSSGEPPEKQPEI 121
             DK D PGTSA  +D+   GGLQSPDAP         VASNPS+SSSSSGEPPEK PEI
Sbjct: 66  -DDKPDLPGTSALAVDE--TGGLQSPDAPV-------PVASNPSMSSSSSGEPPEKLPEI 125

Query: 122 PRKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTNSKCT 181
           P+KVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVK SPFPRSYYRCTNSKCT
Sbjct: 126 PQKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKYSPFPRSYYRCTNSKCT 185

Query: 182 VKKRVERSSEDPTVVITTYEGQHCHHTVGFPRGGLNMAHEAAFGGQFSPQMAQFYYPEAQ 241
           VKKRVERSSEDP+VVITTYEGQHCHHTVGFPRGGLNMAH+AAFG QFSPQ A  YY E Q
Sbjct: 186 VKKRVERSSEDPSVVITTYEGQHCHHTVGFPRGGLNMAHKAAFGSQFSPQGAHIYYHETQ 245

Query: 242 PLPRNNPPN-------AHQQQILPTMA---SSNA-AFEQKEPANSNSQPVPSDEGLLGDI 299
           PLPRNN  N        H  Q L T A   SSNA + EQ+EP NS  Q V SDEGLLGDI
Sbjct: 246 PLPRNNNNNNDTPTARDHHSQHLSTAAASSSSNALSLEQQEPTNSQPQ-VASDEGLLGDI 299

BLAST of Lag0004756 vs. NCBI nr
Match: XP_022997226.1 (probable WRKY transcription factor 57 isoform X1 [Cucurbita maxima] >XP_022997227.1 probable WRKY transcription factor 57 isoform X1 [Cucurbita maxima])

HSP 1 Score: 408.7 bits (1049), Expect = 4.4e-110
Identity = 229/307 (74.59%), Postives = 241/307 (78.50%), Query Frame = 0

Query: 2   DPDPPPDFSPADSSTDWTNSLASASADDAAYFFSADRDSTILTEFGWNFHSHDLQPPRFL 61
           +PD PP FS ADSST WTN+L SA   D+ YFF ADRDS +LTEFGWNF S D QP RFL
Sbjct: 6   NPDSPPSFSTADSSTIWTNALPSA---DSFYFFPADRDSAVLTEFGWNFLSQDAQPTRFL 65

Query: 62  HSDKADFPGTSAPPIDDGVGGGLQSPDAPATAGSLNDAVASNPSLSSSSSGEPPEKQPEI 121
             DK D PGTS P +D+   GGLQSPDAP         VAS PS+SSSSSGEPPEK PEI
Sbjct: 66  -DDKPDLPGTSEPAVDE--TGGLQSPDAPV-------PVASIPSMSSSSSGEPPEKLPEI 125

Query: 122 PRKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTNSKCT 181
           P+KVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVK SPFPRSYYRCTNSKCT
Sbjct: 126 PQKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKYSPFPRSYYRCTNSKCT 185

Query: 182 VKKRVERSSEDPTVVITTYEGQHCHHTVGFPRGGLNMAHEAAFGGQFSPQMAQFYYPEAQ 241
           VKKRVERSSEDP+VVITTYEGQHCHHTVGFPRGGLNMAH+AAFG QFS Q AQ YYPE Q
Sbjct: 186 VKKRVERSSEDPSVVITTYEGQHCHHTVGFPRGGLNMAHKAAFGSQFSSQGAQIYYPETQ 245

Query: 242 PLPRNNPPN------AHQQQILPTMA---SSNA-AFEQKEPANSNSQPVPSDEGLLGDIV 299
           P PRNN  N       H  Q LPT A   SSNA + EQ+EP NS  Q V SDEGLLGDIV
Sbjct: 246 PPPRNNNNNDTPTARDHHSQHLPTAAASSSSNALSLEQQEPTNSQPQ-VASDEGLLGDIV 298

BLAST of Lag0004756 vs. NCBI nr
Match: XP_022961702.1 (probable WRKY transcription factor 57 [Cucurbita moschata] >XP_022961703.1 probable WRKY transcription factor 57 [Cucurbita moschata])

HSP 1 Score: 408.3 bits (1048), Expect = 5.7e-110
Identity = 228/307 (74.27%), Postives = 241/307 (78.50%), Query Frame = 0

Query: 2   DPDPPPDFSPADSSTDWTNSLASASADDAAYFFSADRDSTILTEFGWNFHSHDLQPPRFL 61
           +PD PP FS ADSST WTN+L SA   D+ YFF ADRDS +LTEFGWNF SHD QP RFL
Sbjct: 6   NPDSPPSFSTADSSTVWTNALPSA---DSFYFFPADRDSAVLTEFGWNFLSHDAQPTRFL 65

Query: 62  HSDKADFPGTSAPPIDDGVGGGLQSPDAPATAGSLNDAVASNPSLSSSSSGEPPEKQPEI 121
             DK D PGTSA  +D+   GGLQSPDAP         VASNPS+SSSSSGEPPEK PEI
Sbjct: 66  -DDKPDLPGTSALAVDE--TGGLQSPDAPV-------PVASNPSMSSSSSGEPPEKLPEI 125

Query: 122 PRKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTNSKCT 181
           P+KVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVK SPFPRSYYRCTNSKCT
Sbjct: 126 PQKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKYSPFPRSYYRCTNSKCT 185

Query: 182 VKKRVERSSEDPTVVITTYEGQHCHHTVGFPRGGLNMAHEAAFGGQFSPQMAQFYYPEAQ 241
           VKKRVERSSEDP+VVITTYEGQHCHHT+GFPRGG NMAH+AAFG QFS Q AQ YYPE Q
Sbjct: 186 VKKRVERSSEDPSVVITTYEGQHCHHTIGFPRGGFNMAHKAAFGSQFSSQGAQIYYPETQ 245

Query: 242 PLPRNNPPN------AHQQQILPTMA---SSNA-AFEQKEPANSNSQPVPSDEGLLGDIV 299
           P PRNN  N       H  Q L T A   SSNA + EQ+EP NS  Q V SDEGLLGDIV
Sbjct: 246 PPPRNNNNNDTPTARDHHSQHLSTAAASSSSNALSLEQQEPTNSQPQ-VASDEGLLGDIV 298

BLAST of Lag0004756 vs. NCBI nr
Match: KAG6598630.1 (putative WRKY transcription factor 57, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 405.2 bits (1040), Expect = 4.8e-109
Identity = 226/307 (73.62%), Postives = 240/307 (78.18%), Query Frame = 0

Query: 2   DPDPPPDFSPADSSTDWTNSLASASADDAAYFFSADRDSTILTEFGWNFHSHDLQPPRFL 61
           +PD PP FS ADSST WTN L SA   ++ YFF ADRDS +LTEFGWNF SHD QP RFL
Sbjct: 6   NPDSPPSFSTADSSTVWTNVLPSA---ESFYFFPADRDSAVLTEFGWNFLSHDAQPTRFL 65

Query: 62  HSDKADFPGTSAPPIDDGVGGGLQSPDAPATAGSLNDAVASNPSLSSSSSGEPPEKQPEI 121
             DK D PGTSA  +  G  GGLQSPDAP         VASNPS+SSSSSGEPPEK PEI
Sbjct: 66  -DDKPDLPGTSALAV--GETGGLQSPDAPV-------PVASNPSMSSSSSGEPPEKLPEI 125

Query: 122 PRKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTNSKCT 181
           P+K+KKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVK SPFPRSYYRCTNSKCT
Sbjct: 126 PQKIKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKYSPFPRSYYRCTNSKCT 185

Query: 182 VKKRVERSSEDPTVVITTYEGQHCHHTVGFPRGGLNMAHEAAFGGQFSPQMAQFYYPEAQ 241
           VKKRVERSSEDP+VVITTYEGQHCHH++GFPRGG NMAH+AAFG QFS Q AQ YYPE Q
Sbjct: 186 VKKRVERSSEDPSVVITTYEGQHCHHSIGFPRGGFNMAHKAAFGSQFSSQGAQIYYPETQ 245

Query: 242 PLPRNNPPN------AHQQQILPTMA---SSNA-AFEQKEPANSNSQPVPSDEGLLGDIV 299
           P PRNN  N       H  Q LPT A   SSNA + EQ+EP NS  Q V SDEGLLGDIV
Sbjct: 246 PPPRNNNNNDTPTARDHHSQHLPTAAASSSSNALSLEQQEPTNSQPQ-VASDEGLLGDIV 298

BLAST of Lag0004756 vs. NCBI nr
Match: KAG7029566.1 (putative WRKY transcription factor 57, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 397.1 bits (1019), Expect = 1.3e-106
Identity = 227/321 (70.72%), Postives = 240/321 (74.77%), Query Frame = 0

Query: 2   DPDPPPDFSPADSSTDWTNSLASASADDAAYFFSADRDSTILTEFGWNFHSHDLQPPRFL 61
           +PD PP FS ADSST WTN L SA   ++ YFF ADRDS +LTEFGWNF SHD QP RFL
Sbjct: 6   NPDSPPSFSTADSSTVWTNVLPSA---ESFYFFPADRDSAVLTEFGWNFLSHDAQPTRFL 65

Query: 62  HSDKADFPGTSAPPIDDGVGGGLQSPDAPATAGSLNDAVASNPSLSSSSSGEPPEKQPEI 121
             DK D PGTSA  +  G  GGLQSPDAP         VASNPS+SSSSSGEPPEK PEI
Sbjct: 66  -DDKPDLPGTSALAV--GETGGLQSPDAPV-------PVASNPSMSSSSSGEPPEKLPEI 125

Query: 122 P--------------RKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSP 181
           P              +K+KKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVK SP
Sbjct: 126 PICFYFTNVGEIFCRQKIKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKYSP 185

Query: 182 FPRSYYRCTNSKCTVKKRVERSSEDPTVVITTYEGQHCHHTVGFPRGGLNMAHEAAFGGQ 241
           FPRSYYRCTNSKCTVKKRVERSSEDP+VVITTYEGQHCHHT+GFPRGG NMAH+AAFG Q
Sbjct: 186 FPRSYYRCTNSKCTVKKRVERSSEDPSVVITTYEGQHCHHTIGFPRGGFNMAHKAAFGSQ 245

Query: 242 FSPQMAQFYYPEAQPLPRNNPPN------AHQQQILPTMA---SSNA-AFEQKEPANSNS 299
           FS Q AQ YYPE QP PRNN  N       H  Q LPT A   SSNA + EQ+EP NS  
Sbjct: 246 FSSQGAQIYYPETQPPPRNNNNNDTPTARDHHSQHLPTAAASSSSNALSLEQQEPTNSQP 305

BLAST of Lag0004756 vs. ExPASy Swiss-Prot
Match: Q9C983 (Probable WRKY transcription factor 57 OS=Arabidopsis thaliana OX=3702 GN=WRKY57 PE=2 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 9.9e-57
Identity = 153/309 (49.51%), Postives = 186/309 (60.19%), Query Frame = 0

Query: 2   DPDPPPDFSPADSSTDWTNSLASASADDAAYFFSADRD-STILTEFGWNFHSHDLQPPRF 61
           DPD  PD S  DS+  W     + +A D+ +F   DRD S IL++FGWN H H    P  
Sbjct: 3   DPD-NPDLSNDDSA--WRE--LTLTAQDSDFF---DRDTSNILSDFGWNLH-HSSDHPHS 62

Query: 62  LHSDKADFPGTSAPPIDDGVGGGLQSPDAPATAGSLNDAVASNPSLSSSSSGEPPEKQ-- 121
           L  D      T   P    V     S  A + A +   +  +NPS +SSSS +P E    
Sbjct: 63  LRFDSDLTQTTGVKPTT--VTSSCSSSAAVSVAVT---STNNNPSATSSSSEDPAENSTA 122

Query: 122 -------PEIPRKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRS 181
                  PE P K KKK QKRIRQPRFAFMTKS+VD+LEDGYRWRKYGQKAVKNSPFPRS
Sbjct: 123 SAEKTPPPETPVKEKKKAQKRIRQPRFAFMTKSDVDNLEDGYRWRKYGQKAVKNSPFPRS 182

Query: 182 YYRCTNSKCTVKKRVERSSEDPTVVITTYEGQHCHHTVGFPRGGLNMAHEAAFGGQFSPQ 241
           YYRCTNS+CTVKKRVERSS+DP++VITTYEGQHCH T+GFPRGG+  AH+        P 
Sbjct: 183 YYRCTNSRCTVKKRVERSSDDPSIVITTYEGQHCHQTIGFPRGGILTAHD--------PH 242

Query: 242 MAQFYYPEAQPLPRNNPPNAHQQQILPTMASSNAAFEQKEP----ANSNSQPVPSDEGLL 297
               ++    PLP  NP   + Q++L  +   N A   + P     ++ +   PS+EGLL
Sbjct: 243 SFTSHHHLPPPLP--NP--YYYQELLHQLHRDNNAPSPRLPRPTTEDTPAVSTPSEEGLL 285

BLAST of Lag0004756 vs. ExPASy Swiss-Prot
Match: Q9FGZ4 (Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.5e-33
Identity = 94/239 (39.33%), Postives = 133/239 (55.65%), Query Frame = 0

Query: 86  SPDAPATAGSLNDAVASNPS-----LSSSSSGEPPEKQ----PEIPRKVKKKGQKRIRQP 145
           SP++ + + S N+A   N S     +     G+  ++Q    P++  K KKK QK+ R+ 
Sbjct: 150 SPNSTSVSSSSNEAANDNNSGKEVTVKDQEEGDQQQEQKGTKPQL--KAKKKNQKKAREA 209

Query: 146 RFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTNSKCTVKKRVERSSEDPTVV 205
           RFAF+TKS++D+L+DGYRWRKYGQKAVKNSP+PRSYYRCT   C VKKRVERSS+DP++V
Sbjct: 210 RFAFLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKKRVERSSDDPSIV 269

Query: 206 ITTYEGQHCHHTVGFPRGGLNMAHEAAFG-GQFSPQMAQFYYPEAQPL--PRNNPPNAHQ 265
           +TTYEGQH H     PRG + M        G  +   + F  P+ + L    + P N + 
Sbjct: 270 MTTYEGQHTHPFPMTPRGHIGMLTSPILDHGATTASSSSFSIPQPRYLLTQHHQPYNMYN 329

Query: 266 QQIL----------------PTMASSNAAFEQKEPANSNSQPVPSDEGLLGDIVPPGMR 297
              L                P+ +     ++  + + S S  +  D GLL DI+P  +R
Sbjct: 330 NNSLSMINRRSSDGTFVNPGPSSSFPGFGYDMSQASTSTSSSI-RDHGLLQDILPSQIR 385

BLAST of Lag0004756 vs. ExPASy Swiss-Prot
Match: Q93WV4 (WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.3e-32
Identity = 96/230 (41.74%), Postives = 127/230 (55.22%), Query Frame = 0

Query: 89  APATAGSLNDAVASNPSLSSSSS-GEPP---------------------EKQPEIPRKVK 148
           +P+      +  +++P +SSSS+ GEP                      E   ++ ++ K
Sbjct: 53  SPSVNNPYLNLTSNSPVVSSSSNEGEPKENTNDKSDQMEDNEGDLHGVGESSKQLTKQGK 112

Query: 149 KKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTNSKCTVKKRV 208
           KKG+K+ R+ R AFMTKSE+DHLEDGYRWRKYGQKAVKNSP+PRSYYRCT  KC VKKRV
Sbjct: 113 KKGEKKEREVRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRV 172

Query: 209 ERSSEDPTVVITTYEGQHCHHTVGFPRGGLNMAHEAAFGGQFSPQMAQFYYPEAQPLPRN 268
           ERS +DP++VITTYEG+H H      RG +   H     G     +  F       L   
Sbjct: 173 ERSFQDPSIVITTYEGKHNHPIPSTLRGTVAAEHLLVHRGGGGSLLHSFPRHHQDFLMMK 232

Query: 269 NPPNAHQQQILPTMASSNAAFEQKEPANS----NSQPVPSDEGLLGDIVP 293
           + P  +Q       +  + ++E     +S    N+QPV  D GLL DIVP
Sbjct: 233 HSPANYQ-------SVGSLSYEHGHGTSSYNFNNNQPV-VDYGLLQDIVP 274

BLAST of Lag0004756 vs. ExPASy Swiss-Prot
Match: O22900 (WRKY transcription factor 23 OS=Arabidopsis thaliana OX=3702 GN=WRKY23 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 8.5e-32
Identity = 101/237 (42.62%), Postives = 126/237 (53.16%), Query Frame = 0

Query: 90  PATAGSLNDAVASNPSL---------SSSSSGEPPEKQPEIPR--KVKKKGQKRIRQPRF 149
           PAT  S + + AS+ +L         +    GE  +++    +  K KK  QKR R+ R 
Sbjct: 103 PATPNSSSISSASSEALNEEKPKTEDNEEEGGEDQQEKSHTKKQLKAKKNNQKRQREARV 162

Query: 150 AFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTNSKCTVKKRVERSSEDPTVVIT 209
           AFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCT + C VKKRVERS  DP+ V+T
Sbjct: 163 AFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTTASCNVKKRVERSFRDPSTVVT 222

Query: 210 TYEGQHCH---------HTVGF--PRGGLNMAHEAAFGGQFSPQMAQFYYPEAQPLPRNN 269
           TYEGQH H          T GF    G  +      FG  F    +    P+ Q L + +
Sbjct: 223 TYEGQHTHISPLTSRPISTGGFFGSSGAASSLGNGCFG--FPIDGSTLISPQFQQLVQYH 282

Query: 270 PPNAHQQQILPTMA---------SSNAAFEQKEPANSNSQPVPSDEGLLGDIVPPGM 296
               HQQQ    M+         +S+A     +     S+ +  D GLL D+VP  M
Sbjct: 283 ----HQQQQQELMSCFGGVNEYLNSHANEYGDDNRVKKSRVLVKDNGLLQDVVPSHM 333

BLAST of Lag0004756 vs. ExPASy Swiss-Prot
Match: Q8VWJ2 (WRKY transcription factor 28 OS=Arabidopsis thaliana OX=3702 GN=WRKY28 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.1e-31
Identity = 76/132 (57.58%), Postives = 91/132 (68.94%), Query Frame = 0

Query: 81  GGGLQSPDAPATAGSLNDAVASNPSLSSSSSGEPPE---KQPEIPRKV---KKKGQKRIR 140
           GG     +   +  + + + A +P   S  S    E   ++ +I +KV   KK   K+ R
Sbjct: 97  GGACNETETRVSPSNSSSSEADHPGEDSGKSRRKRELVGEEDQISKKVGKTKKTEVKKQR 156

Query: 141 QPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTNSKCTVKKRVERSSEDPT 200
           +PR +FMTKSEVDHLEDGYRWRKYGQKAVKNSP+PRSYYRCT  KC VKKRVERS +DPT
Sbjct: 157 EPRVSFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSFQDPT 216

Query: 201 VVITTYEGQHCH 207
           VVITTYEGQH H
Sbjct: 217 VVITTYEGQHNH 228

BLAST of Lag0004756 vs. ExPASy TrEMBL
Match: A0A6J1K4F6 (probable WRKY transcription factor 57 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492193 PE=4 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 2.1e-110
Identity = 229/307 (74.59%), Postives = 241/307 (78.50%), Query Frame = 0

Query: 2   DPDPPPDFSPADSSTDWTNSLASASADDAAYFFSADRDSTILTEFGWNFHSHDLQPPRFL 61
           +PD PP FS ADSST WTN+L SA   D+ YFF ADRDS +LTEFGWNF S D QP RFL
Sbjct: 6   NPDSPPSFSTADSSTIWTNALPSA---DSFYFFPADRDSAVLTEFGWNFLSQDAQPTRFL 65

Query: 62  HSDKADFPGTSAPPIDDGVGGGLQSPDAPATAGSLNDAVASNPSLSSSSSGEPPEKQPEI 121
             DK D PGTS P +D+   GGLQSPDAP         VAS PS+SSSSSGEPPEK PEI
Sbjct: 66  -DDKPDLPGTSEPAVDE--TGGLQSPDAPV-------PVASIPSMSSSSSGEPPEKLPEI 125

Query: 122 PRKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTNSKCT 181
           P+KVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVK SPFPRSYYRCTNSKCT
Sbjct: 126 PQKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKYSPFPRSYYRCTNSKCT 185

Query: 182 VKKRVERSSEDPTVVITTYEGQHCHHTVGFPRGGLNMAHEAAFGGQFSPQMAQFYYPEAQ 241
           VKKRVERSSEDP+VVITTYEGQHCHHTVGFPRGGLNMAH+AAFG QFS Q AQ YYPE Q
Sbjct: 186 VKKRVERSSEDPSVVITTYEGQHCHHTVGFPRGGLNMAHKAAFGSQFSSQGAQIYYPETQ 245

Query: 242 PLPRNNPPN------AHQQQILPTMA---SSNA-AFEQKEPANSNSQPVPSDEGLLGDIV 299
           P PRNN  N       H  Q LPT A   SSNA + EQ+EP NS  Q V SDEGLLGDIV
Sbjct: 246 PPPRNNNNNDTPTARDHHSQHLPTAAASSSSNALSLEQQEPTNSQPQ-VASDEGLLGDIV 298

BLAST of Lag0004756 vs. ExPASy TrEMBL
Match: A0A6J1HAV4 (probable WRKY transcription factor 57 OS=Cucurbita moschata OX=3662 GN=LOC111462394 PE=4 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 2.8e-110
Identity = 228/307 (74.27%), Postives = 241/307 (78.50%), Query Frame = 0

Query: 2   DPDPPPDFSPADSSTDWTNSLASASADDAAYFFSADRDSTILTEFGWNFHSHDLQPPRFL 61
           +PD PP FS ADSST WTN+L SA   D+ YFF ADRDS +LTEFGWNF SHD QP RFL
Sbjct: 6   NPDSPPSFSTADSSTVWTNALPSA---DSFYFFPADRDSAVLTEFGWNFLSHDAQPTRFL 65

Query: 62  HSDKADFPGTSAPPIDDGVGGGLQSPDAPATAGSLNDAVASNPSLSSSSSGEPPEKQPEI 121
             DK D PGTSA  +D+   GGLQSPDAP         VASNPS+SSSSSGEPPEK PEI
Sbjct: 66  -DDKPDLPGTSALAVDE--TGGLQSPDAPV-------PVASNPSMSSSSSGEPPEKLPEI 125

Query: 122 PRKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTNSKCT 181
           P+KVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVK SPFPRSYYRCTNSKCT
Sbjct: 126 PQKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKYSPFPRSYYRCTNSKCT 185

Query: 182 VKKRVERSSEDPTVVITTYEGQHCHHTVGFPRGGLNMAHEAAFGGQFSPQMAQFYYPEAQ 241
           VKKRVERSSEDP+VVITTYEGQHCHHT+GFPRGG NMAH+AAFG QFS Q AQ YYPE Q
Sbjct: 186 VKKRVERSSEDPSVVITTYEGQHCHHTIGFPRGGFNMAHKAAFGSQFSSQGAQIYYPETQ 245

Query: 242 PLPRNNPPN------AHQQQILPTMA---SSNA-AFEQKEPANSNSQPVPSDEGLLGDIV 299
           P PRNN  N       H  Q L T A   SSNA + EQ+EP NS  Q V SDEGLLGDIV
Sbjct: 246 PPPRNNNNNDTPTARDHHSQHLSTAAASSSSNALSLEQQEPTNSQPQ-VASDEGLLGDIV 298

BLAST of Lag0004756 vs. ExPASy TrEMBL
Match: A0A6J1GGN1 (probable WRKY transcription factor 57 OS=Cucurbita moschata OX=3662 GN=LOC111454025 PE=4 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 3.5e-105
Identity = 219/306 (71.57%), Postives = 237/306 (77.45%), Query Frame = 0

Query: 1   MDPDPPPDFSPADSSTDWTNSLASASADDAAYFFSADRDSTILTEFGWNFHSHDLQPPRF 60
           MD DPPP+FS ADSST+WT+S  ++++ DA Y  S D ++TILTEFGWNF S ++QP RF
Sbjct: 1   MDRDPPPNFSTADSSTNWTSSSLASASVDADYLLSGDSETTILTEFGWNFDSDEVQPSRF 60

Query: 61  LHSDK------ADFPGTSAPPIDDGVGGGLQSPDAPATAGSLNDAVASNPSLSSSSSGEP 120
           L SD+      ADF GTS         G LQSPD P         +ASNPSLSSSSSGEP
Sbjct: 61  LDSDQIATDYNADFAGTST--------GSLQSPDVPV-------PLASNPSLSSSSSGEP 120

Query: 121 PEKQPEIPR-KVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYY 180
           PEKQPEIPR KVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYY
Sbjct: 121 PEKQPEIPRNKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYY 180

Query: 181 RCTNSKCTVKKRVERSSEDPTVVITTYEGQHCHHTVGFPR-GGLNMAHEAAFGGQFSPQM 240
           RCTNSKCTVKKRVERSSEDPTVVITTYEGQHCHHTVGFPR GGLNMAHE AFG QFSP M
Sbjct: 181 RCTNSKCTVKKRVERSSEDPTVVITTYEGQHCHHTVGFPRGGGLNMAHEPAFGTQFSPHM 240

Query: 241 AQFYYPEAQPLPRNNPPNAHQQQILPTMASSNAAFEQKEPANSNSQPVPSDEGLLGDIVP 299
           AQF+YPE  P P   PPNA     LP++A S     Q EP NS+ Q V SDEGLLGD+V 
Sbjct: 241 AQFFYPEPHPPPTITPPNAPN---LPSVAPS-----QAEPTNSSPQ-VASDEGLLGDVVA 282

BLAST of Lag0004756 vs. ExPASy TrEMBL
Match: A0A6J1KRH0 (probable WRKY transcription factor 57 OS=Cucurbita maxima OX=3661 GN=LOC111496552 PE=4 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 3.1e-101
Identity = 214/308 (69.48%), Postives = 238/308 (77.27%), Query Frame = 0

Query: 1   MDPDPPPDFSPADSSTDWTNSLASASADDAAYFFSADRDSTILTEFGWNFHSHDLQPPRF 60
           MD DPPP+FS  DSS++WT+S  ++++ DA Y  S DR+++ILTEFGWNF S ++QP RF
Sbjct: 1   MDRDPPPNFSTNDSSSNWTSSSLASASVDADYLLSGDRETSILTEFGWNFDSDEVQPSRF 60

Query: 61  LHSDK------ADFPGTSAPPIDDGVGGGLQSPDAPATAGSLNDAVASNPSLSSSSSGEP 120
           L SD+      ADF GTS         G LQSPD P         +ASNPSLSSSSSGE 
Sbjct: 61  LDSDQIATDDNADFAGTST--------GSLQSPDVPV-------PLASNPSLSSSSSGEL 120

Query: 121 PEKQPEIPR-KVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYY 180
           PEKQPEIPR KVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYY
Sbjct: 121 PEKQPEIPRNKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYY 180

Query: 181 RCTNSKCTVKKRVERSSEDPTVVITTYEGQHCHHTVGFPR-GGLNMAHEAAFGGQFSPQM 240
           RCTNSKCTVKKRVERSSEDPTVVITTYEGQHCHHTVGFPR GGLN+ HEAAFG QFSP M
Sbjct: 181 RCTNSKCTVKKRVERSSEDPTVVITTYEGQHCHHTVGFPRGGGLNLGHEAAFGTQFSPHM 240

Query: 241 --AQFYYPEAQPLPRNNPPNAHQQQILPTMASSNAAFEQKEPANSNSQPVPSDEGLLGDI 299
             AQF+YP+  P P  +PPNA     LP++A S     Q+EP NS+ Q V SDEGLLGD+
Sbjct: 241 AQAQFFYPDPHPPPTISPPNAPPN--LPSVAPS-----QEEPTNSSPQ-VASDEGLLGDV 285

BLAST of Lag0004756 vs. ExPASy TrEMBL
Match: A0A1S3BDM9 (probable WRKY transcription factor 57 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488478 PE=4 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 1.9e-90
Identity = 196/313 (62.62%), Postives = 220/313 (70.29%), Query Frame = 0

Query: 3   PDPPPDFSPADSSTDWTNSLASASADDAAYFFSADRDSTILTEFGWNFHSHDLQPPRF-- 62
           P PPP FS ADSS+  +N+L         +FF  D DSTILT+FGWNFHS   QP RF  
Sbjct: 7   PPPPPPFSTADSSSTSSNAL---------FFFPPDSDSTILTQFGWNFHSLQPQPSRFHD 66

Query: 63  LHSDKADFPGTSAPPIDDGVGGGLQSPDAPATAGS--LNDA---VASNPSLSSSSSGEPP 122
            H   +DFP TS             +   P    S  L+DA   + SNPSLSSSSSG+PP
Sbjct: 67  SHPIHSDFPATSTTTTTTTTTTTTITTTPPLIEDSAGLSDAPLPLPSNPSLSSSSSGDPP 126

Query: 123 EKQPEI--PRKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYY 182
           +K PEI  PRKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYY
Sbjct: 127 DKPPEIITPRKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYY 186

Query: 183 RCTNSKCTVKKRVERSSEDPTVVITTYEGQHCHHTVGFPRGGLNMAHEAAFGGQFSPQMA 242
           RCTNSKCTVKKRVERS EDP+VVITTYEGQHCHHTVGFPRGGL +AHE +FG QFSPQ+ 
Sbjct: 187 RCTNSKCTVKKRVERSCEDPSVVITTYEGQHCHHTVGFPRGGLTIAHETSFGSQFSPQIP 246

Query: 243 QFYYPEAQPLP--------RNNPPNAHQQQILPTMASSNAAFEQKEPANSNSQPVPSDEG 299
            F+YP+  P P         +NPP     Q  P+ +SS    EQ+EP NS+ Q +PS+EG
Sbjct: 247 HFFYPDPSPPPPPPPTTTNNHNPPTPPIDQHFPSTSSST---EQQEPPNSHLQQLPSNEG 306

BLAST of Lag0004756 vs. TAIR 10
Match: AT1G69310.1 (WRKY DNA-binding protein 57 )

HSP 1 Score: 221.9 bits (564), Expect = 7.1e-58
Identity = 153/309 (49.51%), Postives = 186/309 (60.19%), Query Frame = 0

Query: 2   DPDPPPDFSPADSSTDWTNSLASASADDAAYFFSADRD-STILTEFGWNFHSHDLQPPRF 61
           DPD  PD S  DS+  W     + +A D+ +F   DRD S IL++FGWN H H    P  
Sbjct: 3   DPD-NPDLSNDDSA--WRE--LTLTAQDSDFF---DRDTSNILSDFGWNLH-HSSDHPHS 62

Query: 62  LHSDKADFPGTSAPPIDDGVGGGLQSPDAPATAGSLNDAVASNPSLSSSSSGEPPEKQ-- 121
           L  D      T   P    V     S  A + A +   +  +NPS +SSSS +P E    
Sbjct: 63  LRFDSDLTQTTGVKPTT--VTSSCSSSAAVSVAVT---STNNNPSATSSSSEDPAENSTA 122

Query: 122 -------PEIPRKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRS 181
                  PE P K KKK QKRIRQPRFAFMTKS+VD+LEDGYRWRKYGQKAVKNSPFPRS
Sbjct: 123 SAEKTPPPETPVKEKKKAQKRIRQPRFAFMTKSDVDNLEDGYRWRKYGQKAVKNSPFPRS 182

Query: 182 YYRCTNSKCTVKKRVERSSEDPTVVITTYEGQHCHHTVGFPRGGLNMAHEAAFGGQFSPQ 241
           YYRCTNS+CTVKKRVERSS+DP++VITTYEGQHCH T+GFPRGG+  AH+        P 
Sbjct: 183 YYRCTNSRCTVKKRVERSSDDPSIVITTYEGQHCHQTIGFPRGGILTAHD--------PH 242

Query: 242 MAQFYYPEAQPLPRNNPPNAHQQQILPTMASSNAAFEQKEP----ANSNSQPVPSDEGLL 297
               ++    PLP  NP   + Q++L  +   N A   + P     ++ +   PS+EGLL
Sbjct: 243 SFTSHHHLPPPLP--NP--YYYQELLHQLHRDNNAPSPRLPRPTTEDTPAVSTPSEEGLL 285

BLAST of Lag0004756 vs. TAIR 10
Match: AT1G69310.2 (WRKY DNA-binding protein 57 )

HSP 1 Score: 221.9 bits (564), Expect = 7.1e-58
Identity = 153/309 (49.51%), Postives = 186/309 (60.19%), Query Frame = 0

Query: 2   DPDPPPDFSPADSSTDWTNSLASASADDAAYFFSADRD-STILTEFGWNFHSHDLQPPRF 61
           DPD  PD S  DS+  W     + +A D+ +F   DRD S IL++FGWN H H    P  
Sbjct: 3   DPD-NPDLSNDDSA--WRE--LTLTAQDSDFF---DRDTSNILSDFGWNLH-HSSDHPHS 62

Query: 62  LHSDKADFPGTSAPPIDDGVGGGLQSPDAPATAGSLNDAVASNPSLSSSSSGEPPEKQ-- 121
           L  D      T   P    V     S  A + A +   +  +NPS +SSSS +P E    
Sbjct: 63  LRFDSDLTQTTGVKPTT--VTSSCSSSAAVSVAVT---STNNNPSATSSSSEDPAENSTA 122

Query: 122 -------PEIPRKVKKKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRS 181
                  PE P K KKK QKRIRQPRFAFMTKS+VD+LEDGYRWRKYGQKAVKNSPFPRS
Sbjct: 123 SAEKTPPPETPVKEKKKAQKRIRQPRFAFMTKSDVDNLEDGYRWRKYGQKAVKNSPFPRS 182

Query: 182 YYRCTNSKCTVKKRVERSSEDPTVVITTYEGQHCHHTVGFPRGGLNMAHEAAFGGQFSPQ 241
           YYRCTNS+CTVKKRVERSS+DP++VITTYEGQHCH T+GFPRGG+  AH+        P 
Sbjct: 183 YYRCTNSRCTVKKRVERSSDDPSIVITTYEGQHCHQTIGFPRGGILTAHD--------PH 242

Query: 242 MAQFYYPEAQPLPRNNPPNAHQQQILPTMASSNAAFEQKEP----ANSNSQPVPSDEGLL 297
               ++    PLP  NP   + Q++L  +   N A   + P     ++ +   PS+EGLL
Sbjct: 243 SFTSHHHLPPPLP--NP--YYYQELLHQLHRDNNAPSPRLPRPTTEDTPAVSTPSEEGLL 285

BLAST of Lag0004756 vs. TAIR 10
Match: AT5G49520.1 (WRKY DNA-binding protein 48 )

HSP 1 Score: 144.8 bits (364), Expect = 1.1e-34
Identity = 94/239 (39.33%), Postives = 133/239 (55.65%), Query Frame = 0

Query: 86  SPDAPATAGSLNDAVASNPS-----LSSSSSGEPPEKQ----PEIPRKVKKKGQKRIRQP 145
           SP++ + + S N+A   N S     +     G+  ++Q    P++  K KKK QK+ R+ 
Sbjct: 150 SPNSTSVSSSSNEAANDNNSGKEVTVKDQEEGDQQQEQKGTKPQL--KAKKKNQKKAREA 209

Query: 146 RFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTNSKCTVKKRVERSSEDPTVV 205
           RFAF+TKS++D+L+DGYRWRKYGQKAVKNSP+PRSYYRCT   C VKKRVERSS+DP++V
Sbjct: 210 RFAFLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKKRVERSSDDPSIV 269

Query: 206 ITTYEGQHCHHTVGFPRGGLNMAHEAAFG-GQFSPQMAQFYYPEAQPL--PRNNPPNAHQ 265
           +TTYEGQH H     PRG + M        G  +   + F  P+ + L    + P N + 
Sbjct: 270 MTTYEGQHTHPFPMTPRGHIGMLTSPILDHGATTASSSSFSIPQPRYLLTQHHQPYNMYN 329

Query: 266 QQIL----------------PTMASSNAAFEQKEPANSNSQPVPSDEGLLGDIVPPGMR 297
              L                P+ +     ++  + + S S  +  D GLL DI+P  +R
Sbjct: 330 NNSLSMINRRSSDGTFVNPGPSSSFPGFGYDMSQASTSTSSSI-RDHGLLQDILPSQIR 385

BLAST of Lag0004756 vs. TAIR 10
Match: AT1G29860.1 (WRKY DNA-binding protein 71 )

HSP 1 Score: 141.7 bits (356), Expect = 9.3e-34
Identity = 96/230 (41.74%), Postives = 127/230 (55.22%), Query Frame = 0

Query: 89  APATAGSLNDAVASNPSLSSSSS-GEPP---------------------EKQPEIPRKVK 148
           +P+      +  +++P +SSSS+ GEP                      E   ++ ++ K
Sbjct: 53  SPSVNNPYLNLTSNSPVVSSSSNEGEPKENTNDKSDQMEDNEGDLHGVGESSKQLTKQGK 112

Query: 149 KKGQKRIRQPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTNSKCTVKKRV 208
           KKG+K+ R+ R AFMTKSE+DHLEDGYRWRKYGQKAVKNSP+PRSYYRCT  KC VKKRV
Sbjct: 113 KKGEKKEREVRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRV 172

Query: 209 ERSSEDPTVVITTYEGQHCHHTVGFPRGGLNMAHEAAFGGQFSPQMAQFYYPEAQPLPRN 268
           ERS +DP++VITTYEG+H H      RG +   H     G     +  F       L   
Sbjct: 173 ERSFQDPSIVITTYEGKHNHPIPSTLRGTVAAEHLLVHRGGGGSLLHSFPRHHQDFLMMK 232

Query: 269 NPPNAHQQQILPTMASSNAAFEQKEPANS----NSQPVPSDEGLLGDIVP 293
           + P  +Q       +  + ++E     +S    N+QPV  D GLL DIVP
Sbjct: 233 HSPANYQ-------SVGSLSYEHGHGTSSYNFNNNQPV-VDYGLLQDIVP 274

BLAST of Lag0004756 vs. TAIR 10
Match: AT2G47260.1 (WRKY DNA-binding protein 23 )

HSP 1 Score: 139.0 bits (349), Expect = 6.0e-33
Identity = 101/237 (42.62%), Postives = 126/237 (53.16%), Query Frame = 0

Query: 90  PATAGSLNDAVASNPSL---------SSSSSGEPPEKQPEIPR--KVKKKGQKRIRQPRF 149
           PAT  S + + AS+ +L         +    GE  +++    +  K KK  QKR R+ R 
Sbjct: 103 PATPNSSSISSASSEALNEEKPKTEDNEEEGGEDQQEKSHTKKQLKAKKNNQKRQREARV 162

Query: 150 AFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTNSKCTVKKRVERSSEDPTVVIT 209
           AFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCT + C VKKRVERS  DP+ V+T
Sbjct: 163 AFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTTASCNVKKRVERSFRDPSTVVT 222

Query: 210 TYEGQHCH---------HTVGF--PRGGLNMAHEAAFGGQFSPQMAQFYYPEAQPLPRNN 269
           TYEGQH H          T GF    G  +      FG  F    +    P+ Q L + +
Sbjct: 223 TYEGQHTHISPLTSRPISTGGFFGSSGAASSLGNGCFG--FPIDGSTLISPQFQQLVQYH 282

Query: 270 PPNAHQQQILPTMA---------SSNAAFEQKEPANSNSQPVPSDEGLLGDIVPPGM 296
               HQQQ    M+         +S+A     +     S+ +  D GLL D+VP  M
Sbjct: 283 ----HQQQQQELMSCFGGVNEYLNSHANEYGDDNRVKKSRVLVKDNGLLQDVVPSHM 333