Lag0036958 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0036958 vs. NCBI nr
Match: XP_038899591.1 (triacylglycerol lipase OBL1-like [Benincasa hispida])

HSP 1 Score: 728.0 bits (1878), Expect = 5.3e-206
Identity = 356/482 (73.86%), Postives = 408/482 (84.65%), Query Frame = 0

Query: 6   EHAFSRNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKGIEDSLRKFTDRLSIVISM 65
           E  FS+NY+ILKPENAN+ DLFLFLLPFG  R+R+FIDC  G ED  + F DR  I ISM
Sbjct: 5   ESEFSKNYVILKPENANLLDLFLFLLPFG-LRKRKFIDCPLGKEDPFKNFRDRWLIFISM 64

Query: 66  VLQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLVWPDKSSPEYMSLA 125
           +LQ IL   ATPLAKLDSFLL L NFISFNGGV G+  KIL  K  +  ++ SPEY S+ 
Sbjct: 65  LLQFILAIIATPLAKLDSFLLKLLNFISFNGGVSGILSKILKGKSFLEVNEDSPEYTSVV 124

Query: 126 AFTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWN 185
            F DWRRDLDSSI+P DTFRYYSALTVMAAK+SYE +P+VQSVVN RW+M LLGYF+FWN
Sbjct: 125 GFADWRRDLDSSIKPDDTFRYYSALTVMAAKVSYESKPFVQSVVNDRWKMKLLGYFNFWN 184

Query: 186 DFQRKATTQAFMFETSATDPNVTVVAFRGTSPLDTYDWQVDADFSWYEIEGVGRMHSGFM 245
           DFQ KATTQAFMFE +A DPNV VVAFRGTS LD+YDWQVDADFSWYEIEGVGR+HSGFM
Sbjct: 185 DFQGKATTQAFMFENTAIDPNVIVVAFRGTSLLDSYDWQVDADFSWYEIEGVGRIHSGFM 244

Query: 246 KALGLQKATGWPKELPGPQQHDFAYYTLRQKLRDIATANHNARFIITGHSLGGALATVFV 305
           KALGLQKATGWPKELP  Q  +FAYYT+RQKLRDIA  N  ARFIITGHSLGGALAT+FV
Sbjct: 245 KALGLQKATGWPKELPKSQLQEFAYYTIRQKLRDIAKTNDKARFIITGHSLGGALATLFV 304

Query: 306 TILALHHESSILEKLEAVYTYGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPRVP 365
           T+L+LH ES+ILEKLE +YTYGQPRVG++ FAKFMV+TVQKYGFKY+RYVYSSDLVPR+P
Sbjct: 305 TVLSLHDESTILEKLEGIYTYGQPRVGDQQFAKFMVNTVQKYGFKYHRYVYSSDLVPRLP 364

Query: 366 FDAIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPRFLSAWWEVIRSWIIPFV 425
            DAI FKY+HFG+ V+FN+LYRGRI+KEQP KNY SL W IP++LSAWWE+IRS IIP V
Sbjct: 365 SDAILFKYKHFGRCVFFNTLYRGRIVKEQPNKNYFSLVWVIPKYLSAWWELIRSLIIPLV 424

Query: 426 RGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDIEP 485
           +GFDYYE+LLM+G RVVGL +PG+AAHFP+NYVN TRLGKL +    ++E+PIL DDIE 
Sbjct: 425 KGFDYYESLLMKGGRVVGLFIPGLAAHFPLNYVNSTRLGKLNV--PDKVEDPILGDDIED 483

Query: 486 DY 488
           D+
Sbjct: 485 DF 483

BLAST of Lag0036958 vs. NCBI nr
Match: XP_023523371.1 (uncharacterized protein LOC111787588 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 721.5 bits (1861), Expect = 5.0e-204
Identity = 351/484 (72.52%), Postives = 412/484 (85.12%), Query Frame = 0

Query: 5   QEHAFSRNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKGIEDSLRKFTDRLSIVIS 64
           +E  FS+NY+ILKP+NA + DLFLFLLPFG  R+ +FIDC KG EDS   FTDR SI IS
Sbjct: 4   KECEFSKNYMILKPDNAKLLDLFLFLLPFG-LRKTKFIDCPKGYEDSFNNFTDRWSIFIS 63

Query: 65  MVLQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLVWPDKSSPEYMSL 124
           ++LQIIL A ATPLAKLDSFL+NLFNFISFNGG+LGL  KIL  K LV P++ S EY S+
Sbjct: 64  VLLQIILAAAATPLAKLDSFLVNLFNFISFNGGILGLLSKILKGKGLVKPNEDSSEYTSV 123

Query: 125 AAFTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFW 184
             F DWR DLDSSI   DTFRYY  LT+MAAKI+YE +P+VQSVVN RW+M LLG+F+FW
Sbjct: 124 LGFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFNFW 183

Query: 185 NDFQRKATTQAFMFETSAT-DPNVTVVAFRGTSPLDTYDWQVDADFSWYEIEGVGRMHSG 244
           NDFQ KATTQAFMF+ +AT DPN+ V+AFRGTSPLDTYDWQVDADFSWY+IEGVGR+HSG
Sbjct: 184 NDFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTYDWQVDADFSWYDIEGVGRIHSG 243

Query: 245 FMKALGLQKATGWPKELPGPQQHDFAYYTLRQKLRDIATANHNARFIITGHSLGGALATV 304
           FMKALGLQKATGWPKEL  P QHDFAYYTLRQKLRDIA AN  ARFIITGHSLGGALAT+
Sbjct: 244 FMKALGLQKATGWPKELTKP-QHDFAYYTLRQKLRDIAKANAKARFIITGHSLGGALATL 303

Query: 305 FVTILALHHESSILEKLEAVYTYGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPR 364
           FVT+L++H E+++LEKL+ VYTYGQPRVG+R FA+FMV++VQKYGFKYYRYVYSSDLVPR
Sbjct: 304 FVTLLSMHDETAVLEKLQGVYTYGQPRVGDRRFAEFMVNSVQKYGFKYYRYVYSSDLVPR 363

Query: 365 VPFDAIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPRFLSAWWEVIRSWIIP 424
           +P D + FKY+HF + +YFNSLY+GRI+KEQP KNY SL W IP++LSAWWE++RS+I P
Sbjct: 364 IPSDGVIFKYKHFARSIYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELVRSFITP 423

Query: 425 FVRGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDI 484
            V GFDYYE+LLM  AR+VGL++PG+AAHFP+NYVN  RLGKL+     ++E+PILEDDI
Sbjct: 424 LVWGFDYYESLLMSAARIVGLIIPGLAAHFPLNYVNSIRLGKLK--ELDEVEDPILEDDI 483

Query: 485 EPDY 488
           E DY
Sbjct: 484 EGDY 483

BLAST of Lag0036958 vs. NCBI nr
Match: XP_011654113.1 (uncharacterized protein LOC101203640 [Cucumis sativus] >KGN55199.1 hypothetical protein Csa_012910 [Cucumis sativus])

HSP 1 Score: 714.9 bits (1844), Expect = 4.7e-202
Identity = 344/482 (71.37%), Postives = 406/482 (84.23%), Query Frame = 0

Query: 6   EHAFSRNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKGIEDSLRKFTDRLSIVISM 65
           E+ FS+NY+ILKPENAN+ DLFLFLLPFG  R+++FIDC  G ED    FTDR  IV+SM
Sbjct: 5   ENEFSKNYVILKPENANLLDLFLFLLPFG-LRKKKFIDCPYGKEDYFTNFTDRWLIVVSM 64

Query: 66  VLQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLVWPDKSSPEYMSLA 125
           +LQ IL   ATPLAKLD+FLL LFNFISFNGG LG+  KIL  K  V  ++ S EY S+ 
Sbjct: 65  LLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYTSVV 124

Query: 126 AFTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWN 185
            F DWRRDLDSSI+P  +FRYYSALT MA KISYE +P+VQSVVN RW+M LLGYF+FWN
Sbjct: 125 GFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFNFWN 184

Query: 186 DFQRKATTQAFMFETSATDPNVTVVAFRGTSPLDTYDWQVDADFSWYEIEGVGRMHSGFM 245
           DFQ + TTQAFMF+ +A DPNVT+VAFRGTSPLD YDWQVD DFSWYEIE VG +HSGFM
Sbjct: 185 DFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHSGFM 244

Query: 246 KALGLQKATGWPKELPGPQQHDFAYYTLRQKLRDIATANHNARFIITGHSLGGALATVFV 305
           KALGLQKATGWPKELP  Q H+FAYYTLR++LRDIA AN  ARFI TGHSLGGALAT+FV
Sbjct: 245 KALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFV 304

Query: 306 TILALHHESSILEKLEAVYTYGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPRVP 365
           T+L LH ES+ILEKL++VYTYGQPRVG++ FAKFM++ +QKYGFKY+RYVYS DLVPRVP
Sbjct: 305 TVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVPRVP 364

Query: 366 FDAIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPRFLSAWWEVIRSWIIPFV 425
            DA+ FKY+HFG+ VYFNSLY+GRI+KEQP KNY SL W IP++LSAWWE+IRS+IIPFV
Sbjct: 365 SDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFIIPFV 424

Query: 426 RGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDIEP 485
           +GFDYYE+LLM+GAR+VGL++PG+ AHFP+NYVN+TRLGKL +    ++E+PIL  +IE 
Sbjct: 425 KGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNV--PDEVEDPILRYEIED 483

Query: 486 DY 488
           DY
Sbjct: 485 DY 483

BLAST of Lag0036958 vs. NCBI nr
Match: XP_022940183.1 (uncharacterized protein LOC111445886 [Cucurbita moschata])

HSP 1 Score: 714.5 bits (1843), Expect = 6.1e-202
Identity = 348/484 (71.90%), Postives = 411/484 (84.92%), Query Frame = 0

Query: 5   QEHAFSRNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKGIEDSLRKFTDRLSIVIS 64
           +E  FS+NY+ILKP +A + DLFLFLLPFG  R+ +FIDC +G EDS   FTDR SI IS
Sbjct: 4   KECEFSKNYMILKPNDAKLLDLFLFLLPFG-LRKTKFIDCPEGNEDSYNNFTDRWSIFIS 63

Query: 65  MVLQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLVWPDKSSPEYMSL 124
           ++LQIIL A ATPLAKLDSFL+NLFNFISFNGG+LGL  KIL  K LV P++ S +Y S+
Sbjct: 64  VLLQIILAAAATPLAKLDSFLVNLFNFISFNGGILGLLAKILKGKGLVKPNEDSSDYTSV 123

Query: 125 AAFTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFW 184
             F DWR DLDSSI   DTFRYY  LT+MAAKI+YE +P+VQSVVN RW+M LLG+F+FW
Sbjct: 124 VGFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFNFW 183

Query: 185 NDFQRKATTQAFMFETSAT-DPNVTVVAFRGTSPLDTYDWQVDADFSWYEIEGVGRMHSG 244
           NDFQ KATTQAFMF+ +AT DPN+ V+AFRGTSPLDTYDWQVDADFSWY+IEGVGR+HSG
Sbjct: 184 NDFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTYDWQVDADFSWYDIEGVGRIHSG 243

Query: 245 FMKALGLQKATGWPKELPGPQQHDFAYYTLRQKLRDIATANHNARFIITGHSLGGALATV 304
           FMKALGLQKATGWPKEL  P QHDFAYYTLRQKLRDIA AN  ARFIITGHSLGGALAT+
Sbjct: 244 FMKALGLQKATGWPKELTKP-QHDFAYYTLRQKLRDIAKANAKARFIITGHSLGGALATL 303

Query: 305 FVTILALHHESSILEKLEAVYTYGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPR 364
           FVT+L+LH E+ ILEKL+ VYTYGQPRVG+R FA+FMV++VQ+YGFKYYRYVYSSDLVPR
Sbjct: 304 FVTLLSLHDETPILEKLQGVYTYGQPRVGDRRFAEFMVNSVQQYGFKYYRYVYSSDLVPR 363

Query: 365 VPFDAIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPRFLSAWWEVIRSWIIP 424
           +P D + FKY+HFG+ +YFN+LY+GRI+K QP KNY SL W IP++LSAWWE++RS+I P
Sbjct: 364 IPSDGVIFKYKHFGRSIYFNTLYKGRIVKGQPNKNYFSLLWVIPKYLSAWWELVRSFITP 423

Query: 425 FVRGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDI 484
            V GFDYYE+LLM  AR++GL++PG+AAHFP+NYVN TRLGKL+     ++E+PILEDDI
Sbjct: 424 LVWGFDYYESLLMSAARIIGLIIPGLAAHFPLNYVNSTRLGKLK--APDEVEDPILEDDI 483

Query: 485 EPDY 488
           E DY
Sbjct: 484 EGDY 483

BLAST of Lag0036958 vs. NCBI nr
Match: XP_008452563.1 (PREDICTED: uncharacterized protein LOC103493549 [Cucumis melo])

HSP 1 Score: 711.8 bits (1836), Expect = 3.9e-201
Identity = 344/482 (71.37%), Postives = 405/482 (84.02%), Query Frame = 0

Query: 6   EHAFSRNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKGIEDSLRKFTDRLSIVISM 65
           E+ FS+NY+ILKPENAN+ DLFLFLLPFG  R+++FIDC  G ED    F DR  I +SM
Sbjct: 5   ENEFSKNYVILKPENANLLDLFLFLLPFG-LRKKKFIDCPYGKEDYFTNFADRWLIFVSM 64

Query: 66  VLQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLVWPDKSSPEYMSLA 125
           +LQ IL   ATPLAKLD+FLL LFNFISFNGG+LG+  KIL  K  V   + S EY S+ 
Sbjct: 65  LLQFILALIATPLAKLDAFLLKLFNFISFNGGLLGILSKILRGKGFVKVKEDSSEYTSVV 124

Query: 126 AFTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWN 185
            F DWRRDLDSSI+   +FRYYSALTVMA KISYE +P+VQSVVN RW+M LLGYF+FWN
Sbjct: 125 GFADWRRDLDSSIKSEKSFRYYSALTVMATKISYESKPFVQSVVNDRWKMKLLGYFNFWN 184

Query: 186 DFQRKATTQAFMFETSATDPNVTVVAFRGTSPLDTYDWQVDADFSWYEIEGVGRMHSGFM 245
           DFQ KATTQAF FE +A DPNV +VAFRGTSPLD YDWQVD D SWYEIEGVGR+HSGFM
Sbjct: 185 DFQGKATTQAFTFENTAIDPNVVIVAFRGTSPLDAYDWQVDVDLSWYEIEGVGRIHSGFM 244

Query: 246 KALGLQKATGWPKELPGPQQHDFAYYTLRQKLRDIATANHNARFIITGHSLGGALATVFV 305
           KALGLQKATGWPKELP  Q H+FAYYTLR++LRDIA AN  ARFI TGHSLGGALAT+FV
Sbjct: 245 KALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFV 304

Query: 306 TILALHHESSILEKLEAVYTYGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPRVP 365
           T+L+LH ES+ILEKL++VYTYGQPRVG++ FAKFMV+ VQKYGFKY+RYVYS DLVPRVP
Sbjct: 305 TVLSLHDESTILEKLDSVYTYGQPRVGDQQFAKFMVNCVQKYGFKYHRYVYSYDLVPRVP 364

Query: 366 FDAIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPRFLSAWWEVIRSWIIPFV 425
            DA+ FKY+HFG+ VYFNSLY+GRI+KEQP +NY SL W IP++LSAWWE+IRS+IIPFV
Sbjct: 365 SDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNRNYFSLLWVIPKYLSAWWELIRSFIIPFV 424

Query: 426 RGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDIEP 485
           +GFDYYE+LLM+GAR+VGLL+PG++AHFP+NYVN+TRLGKL +    ++E+P L  +IE 
Sbjct: 425 KGFDYYESLLMKGARLVGLLIPGLSAHFPLNYVNVTRLGKLNV--PDEVEDPFLGYEIED 483

Query: 486 DY 488
           D+
Sbjct: 485 DF 483

BLAST of Lag0036958 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.2e-77
Identity = 184/546 (33.70%), Postives = 278/546 (50.92%), Query Frame = 0

Query: 13  YLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKG---IEDSLRKFTD------------ 72
           +LI+  EN  ++DL  F +        +F+  S G   +E+ L +  D            
Sbjct: 24  FLIVSHENGGIWDLVRFGVWGNKESGDKFLHYSAGGGLLEEHLVRSDDSGGGDDRGGEVP 83

Query: 73  --RLSIVISMVLQIILLAFATPL---AKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLV 132
             R  I +S++++ ++  F  P+     L  F LNLF   S NG  LGL + IL   K+V
Sbjct: 84  DHRWVIFVSIIVRKLIAIFGKPMEWTGYLVEFFLNLF---SLNGNFLGLLYNILH-GKVV 143

Query: 133 WPDKSSPEYMSLAAFTDWRRDLDSS------IEPSDTF-------RYYSALTVMAAKISY 192
            P + S  ++S     D R +L  S      I   D +       R    L +MA+K++Y
Sbjct: 144 MPHRGSETFISAIGHLDGRINLYKSETLTKEIGEPDFWQKIGIGHRDLMDLCMMASKLAY 203

Query: 193 EFQPYVQSVVNHRWRMNLLGYFDFWNDFQRKATTQAFMFETSATDPNVTVVAFRGTSPLD 252
           E +  V++VVN  W+M+ + +++ WNDF+++ +TQ F+      D N+ +V+FRGT P D
Sbjct: 204 ENEKVVRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDANLILVSFRGTEPFD 263

Query: 253 TYDWQVDADFSWYEIEGVGRMHSGFMKALGLQKATG------------------------ 312
             DW  D D+SWYEI  +G++H GF++ALGL   T                         
Sbjct: 264 ADDWITDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFVNNLKFANLENVHAT 323

Query: 313 WPKELPGPQQHDF----------------------------------AYYTLRQKLRDIA 372
            P          F                                  AYY +R KL+ + 
Sbjct: 324 IPPSESSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPERTAYYVVRSKLKRLL 383

Query: 373 TANHNARFIITGHSLGGALATVFVTILALHHESSILEKLEAVYTYGQPRVGNRDFAKFMV 432
             + NA+F++TGHSLGGALA +F  +L LH E  ++E+L  +YTYGQPRVGNR   +FM 
Sbjct: 384 KEHKNAKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQPRVGNRQLGRFME 443

Query: 433 DTVQKYGFKYYRYVYSSDLVPRVPFDAIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYIS 468
             ++    KY+R VY +DLVPR+P+D   F ++HFG   Y+NSLY  + + E+P  NY  
Sbjct: 444 AHLEHPVPKYFRVVYCNDLVPRLPYDNKTFLFKHFGICQYYNSLYIEQNINEEPNPNYFG 503

BLAST of Lag0036958 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 4.5e-75
Identity = 170/503 (33.80%), Postives = 272/503 (54.08%), Query Frame = 0

Query: 2   GSDQEHAFSRNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKGIEDSLRKFTD---- 61
           GS        NYLI++P      DLF + +      + +F++     E S     +    
Sbjct: 8   GSGSNPGQVSNYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAED 67

Query: 62  -RLSIVISMVLQIILLAFATPLAKLDSFLLNLF-NFISFNGGVLGLFFKILTVKKLVWPD 121
            R  IV+S++++ I+    TP+ +   F+++ F N  S NGG  GL  +++   K+V P+
Sbjct: 68  HRWVIVVSILVRKIIRLLRTPM-EFTGFVVDFFLNLFSANGGFFGLLLRLIQA-KVVIPE 127

Query: 122 KSSPEYMSLAAFTDWRRDLDSS------IEPSDTF-----------RYYSALTVMAAKIS 181
           + S  ++S     D R  L         +E  D+            R    L VMA+K++
Sbjct: 128 RGSVTFVSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLA 187

Query: 182 YEFQPYVQSVVNHRWRMNLLGYFDFWNDFQRKATTQAFMFETSATDPNVTVVAFRGTSPL 241
           YE    V++VV+  W+MNL+ + D WND+Q++ +TQ F+F     D N+ V++FRGT P 
Sbjct: 188 YENAKVVENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPF 247

Query: 242 DTYDWQVDADFSWYEIEGVGRMHSGFMKALGLQ-------------KATGWPKELPGPQQ 301
           D  DW  D D+SWYE+  VG++H GF++A+GL              + T   +E      
Sbjct: 248 DADDWGTDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNL 307

Query: 302 HDF----AYYTLRQKLRDIATANHNARFIITGHSLGGALATVFVTILALHHESSILEKLE 361
            D     AYY +R  L+ + + + NARF++TGHSLGGALA +F T+L L+ E+ I+++L 
Sbjct: 308 LDMVERSAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLL 367

Query: 362 AVYTYGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPRVPFDAIFFKYRHFGKVVY 421
            VYT+GQPR+GNR+   FM   + +   +Y+R VY +D+VPR+P+D   F Y+HFG  ++
Sbjct: 368 GVYTFGQPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLF 427

Query: 422 FNSLYRGRILKEQPYKNYISLFWAIPRFLSAWWEVIRSWIIPFVRGFDYYETLLMRGARV 465
           ++S Y     +++P  N   L + I   + A WE++R   + +  G DY E       R+
Sbjct: 428 YDSFYNETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHGPDYKEGWFRILFRL 487

BLAST of Lag0036958 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 4.4e-70
Identity = 168/507 (33.14%), Postives = 255/507 (50.30%), Query Frame = 0

Query: 10  SRNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKGIEDSLRKFTDR--LSIVISMVL 69
           S ++LI+ P+     DLF  ++        +F   S     +   +  R  +S+ I  +L
Sbjct: 9   STSHLIVSPDEGTFLDLFKHIVLSDLGSGAKFFRASDQRVPATAAYYSRWPVSVFICKIL 68

Query: 70  QIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLVWPDKSSPEYMSLAAF 129
           Q+    F  P A L      L NF   N G LG+   I  + +L  P +   +++S   +
Sbjct: 69  QL----FQMPAAMLGHLTDFLLNFYYQNHGFLGILRNIFLI-RLKIPKRGEADFISTIGY 128

Query: 130 TDWRRDL--------------------------DSSIEPSDTFRYYSALTVMAAKISYEF 189
            D R DL                          +S I+ +   R    L +MA+K++YE 
Sbjct: 129 LDSRMDLHGTPMVSHQADEVISNADNPSLKEGHNSKIKGALGNRSLMDLCIMASKLAYEN 188

Query: 190 QPYVQSVVNHRWRMNLLGYFDFWNDFQRKATTQAFMFETSATDPNVTVVAFRGTSPLDTY 249
              V+ VV   W+M+ +  +   N FQ    T AF+F     D N+ V++FRGT P    
Sbjct: 189 TKVVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIVISFRGTGPFSIP 248

Query: 250 DWQVDADFSWYEIEGVGRMHSGFMKALGL--------------QKATGWPKELPGPQQ-- 309
           +W  D DFS   +   G +H GF++A+GL               K+ G   EL    +  
Sbjct: 249 NWCTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPGSITELRKESEMA 308

Query: 310 -------HDFAYYTLRQKLRDIATANHNARFIITGHSLGGALATVFVTILALHHESSILE 369
                  +D  Y+     L+ +   + NA+F++TGHSLGGALA +F  IL +  E+ +L+
Sbjct: 309 PDHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTCILEIQQETEVLD 368

Query: 370 KLEAVYTYGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPRVPFDAIFFKYRHFGK 429
           +L  VYT+GQPR+GN +   FM + +     +Y+R VY +D+VPRVPFD +FF + HFG 
Sbjct: 369 RLLNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVPRVPFDDVFFTFEHFGT 428

Query: 430 VVYFNSLYRGRILKEQPYKNYISLFWAIPRFLSAWWEVIRSWIIPFVRGFDYYETLLMRG 466
            +Y++S + G   KE+P +N   +  AI   ++AWWE+ RS+I+  V G +Y ET   R 
Sbjct: 429 CIYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNHVYGAEYKETWESRM 488

BLAST of Lag0036958 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 6.0e-11
Identity = 56/160 (35.00%), Postives = 80/160 (50.00%), Query Frame = 0

Query: 209 VVAFRGTSPLDTYDWQVDADFSWYEIEGV---GRMHSGFMKALGLQKATGWPKELPGPQQ 268
           V++FRG+  ++  +W  + +F   EI  +    R H GF         + W         
Sbjct: 99  VLSFRGSRSIE--NWIGNLNFDLKEINDICSGCRGHDGF--------TSSWRSVAD---- 158

Query: 269 HDFAYYTLRQKLRDIATANHNARFIITGHSLGGALATVFVTILALHHESSILEKLEAVYT 328
                 TLRQK+ D    + + R + TGHSLGGALATV    L  +           V++
Sbjct: 159 ------TLRQKVEDAVREHPDYRVVFTGHSLGGALATVAGADLRGNGYDI------DVFS 218

Query: 329 YGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPRVP 366
           YG PRVGNR FA+F+  TVQ  G   YR  +++D+VPR+P
Sbjct: 219 YGAPRVGNRAFAEFL--TVQT-GGTLYRITHTNDIVPRLP 229

BLAST of Lag0036958 vs. ExPASy Swiss-Prot
Match: P61871 (Lipase OS=Rhizopus niveus OX=4844 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 5.3e-07
Identity = 44/156 (28.21%), Postives = 75/156 (48.08%), Query Frame = 0

Query: 210 VAFRGTSPLDTYDWQVDADFSWYEIEGVGRMHSGFMKALGLQKATGWPKELPGPQQHDFA 269
           + FRGT+   +    +  +FS Y+     ++H+GF+ +                +Q    
Sbjct: 201 LVFRGTNSFRSAITDIVFNFSDYKPVKGAKVHAGFLSSY---------------EQVVND 260

Query: 270 YYTLRQKLRDIATANHNARFIITGHSLGGALATVFVTILALHHESSILEKLEAVYTYGQP 329
           Y+ +   +++  TA+   + I+TGHSLGGA A +   +     E  +  K  +++T G P
Sbjct: 261 YFPV---VQEQLTAHPTYKVIVTGHSLGGAQA-LLAGMDLYQREPRLSPKNLSIFTVGGP 320

Query: 330 RVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPRVP 366
           RVGN  FA +    V+  G  + R V+  D+VP VP
Sbjct: 321 RVGNPTFAYY----VESTGIPFQRTVHKRDIVPHVP 333

BLAST of Lag0036958 vs. ExPASy TrEMBL
Match: A0A0A0L2D5 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639870 PE=4 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 2.3e-202
Identity = 344/482 (71.37%), Postives = 406/482 (84.23%), Query Frame = 0

Query: 6   EHAFSRNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKGIEDSLRKFTDRLSIVISM 65
           E+ FS+NY+ILKPENAN+ DLFLFLLPFG  R+++FIDC  G ED    FTDR  IV+SM
Sbjct: 5   ENEFSKNYVILKPENANLLDLFLFLLPFG-LRKKKFIDCPYGKEDYFTNFTDRWLIVVSM 64

Query: 66  VLQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLVWPDKSSPEYMSLA 125
           +LQ IL   ATPLAKLD+FLL LFNFISFNGG LG+  KIL  K  V  ++ S EY S+ 
Sbjct: 65  LLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYTSVV 124

Query: 126 AFTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWN 185
            F DWRRDLDSSI+P  +FRYYSALT MA KISYE +P+VQSVVN RW+M LLGYF+FWN
Sbjct: 125 GFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFNFWN 184

Query: 186 DFQRKATTQAFMFETSATDPNVTVVAFRGTSPLDTYDWQVDADFSWYEIEGVGRMHSGFM 245
           DFQ + TTQAFMF+ +A DPNVT+VAFRGTSPLD YDWQVD DFSWYEIE VG +HSGFM
Sbjct: 185 DFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHSGFM 244

Query: 246 KALGLQKATGWPKELPGPQQHDFAYYTLRQKLRDIATANHNARFIITGHSLGGALATVFV 305
           KALGLQKATGWPKELP  Q H+FAYYTLR++LRDIA AN  ARFI TGHSLGGALAT+FV
Sbjct: 245 KALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFV 304

Query: 306 TILALHHESSILEKLEAVYTYGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPRVP 365
           T+L LH ES+ILEKL++VYTYGQPRVG++ FAKFM++ +QKYGFKY+RYVYS DLVPRVP
Sbjct: 305 TVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLVPRVP 364

Query: 366 FDAIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPRFLSAWWEVIRSWIIPFV 425
            DA+ FKY+HFG+ VYFNSLY+GRI+KEQP KNY SL W IP++LSAWWE+IRS+IIPFV
Sbjct: 365 SDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFIIPFV 424

Query: 426 RGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDIEP 485
           +GFDYYE+LLM+GAR+VGL++PG+ AHFP+NYVN+TRLGKL +    ++E+PIL  +IE 
Sbjct: 425 KGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNV--PDEVEDPILRYEIED 483

Query: 486 DY 488
           DY
Sbjct: 485 DY 483

BLAST of Lag0036958 vs. ExPASy TrEMBL
Match: A0A6J1FNK8 (uncharacterized protein LOC111445886 OS=Cucurbita moschata OX=3662 GN=LOC111445886 PE=4 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 2.9e-202
Identity = 348/484 (71.90%), Postives = 411/484 (84.92%), Query Frame = 0

Query: 5   QEHAFSRNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKGIEDSLRKFTDRLSIVIS 64
           +E  FS+NY+ILKP +A + DLFLFLLPFG  R+ +FIDC +G EDS   FTDR SI IS
Sbjct: 4   KECEFSKNYMILKPNDAKLLDLFLFLLPFG-LRKTKFIDCPEGNEDSYNNFTDRWSIFIS 63

Query: 65  MVLQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLVWPDKSSPEYMSL 124
           ++LQIIL A ATPLAKLDSFL+NLFNFISFNGG+LGL  KIL  K LV P++ S +Y S+
Sbjct: 64  VLLQIILAAAATPLAKLDSFLVNLFNFISFNGGILGLLAKILKGKGLVKPNEDSSDYTSV 123

Query: 125 AAFTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFW 184
             F DWR DLDSSI   DTFRYY  LT+MAAKI+YE +P+VQSVVN RW+M LLG+F+FW
Sbjct: 124 VGFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFNFW 183

Query: 185 NDFQRKATTQAFMFETSAT-DPNVTVVAFRGTSPLDTYDWQVDADFSWYEIEGVGRMHSG 244
           NDFQ KATTQAFMF+ +AT DPN+ V+AFRGTSPLDTYDWQVDADFSWY+IEGVGR+HSG
Sbjct: 184 NDFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTYDWQVDADFSWYDIEGVGRIHSG 243

Query: 245 FMKALGLQKATGWPKELPGPQQHDFAYYTLRQKLRDIATANHNARFIITGHSLGGALATV 304
           FMKALGLQKATGWPKEL  P QHDFAYYTLRQKLRDIA AN  ARFIITGHSLGGALAT+
Sbjct: 244 FMKALGLQKATGWPKELTKP-QHDFAYYTLRQKLRDIAKANAKARFIITGHSLGGALATL 303

Query: 305 FVTILALHHESSILEKLEAVYTYGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPR 364
           FVT+L+LH E+ ILEKL+ VYTYGQPRVG+R FA+FMV++VQ+YGFKYYRYVYSSDLVPR
Sbjct: 304 FVTLLSLHDETPILEKLQGVYTYGQPRVGDRRFAEFMVNSVQQYGFKYYRYVYSSDLVPR 363

Query: 365 VPFDAIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPRFLSAWWEVIRSWIIP 424
           +P D + FKY+HFG+ +YFN+LY+GRI+K QP KNY SL W IP++LSAWWE++RS+I P
Sbjct: 364 IPSDGVIFKYKHFGRSIYFNTLYKGRIVKGQPNKNYFSLLWVIPKYLSAWWELVRSFITP 423

Query: 425 FVRGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDI 484
            V GFDYYE+LLM  AR++GL++PG+AAHFP+NYVN TRLGKL+     ++E+PILEDDI
Sbjct: 424 LVWGFDYYESLLMSAARIIGLIIPGLAAHFPLNYVNSTRLGKLK--APDEVEDPILEDDI 483

Query: 485 EPDY 488
           E DY
Sbjct: 484 EGDY 483

BLAST of Lag0036958 vs. ExPASy TrEMBL
Match: A0A1S3BUY0 (uncharacterized protein LOC103493549 OS=Cucumis melo OX=3656 GN=LOC103493549 PE=4 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 1.9e-201
Identity = 344/482 (71.37%), Postives = 405/482 (84.02%), Query Frame = 0

Query: 6   EHAFSRNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKGIEDSLRKFTDRLSIVISM 65
           E+ FS+NY+ILKPENAN+ DLFLFLLPFG  R+++FIDC  G ED    F DR  I +SM
Sbjct: 5   ENEFSKNYVILKPENANLLDLFLFLLPFG-LRKKKFIDCPYGKEDYFTNFADRWLIFVSM 64

Query: 66  VLQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLVWPDKSSPEYMSLA 125
           +LQ IL   ATPLAKLD+FLL LFNFISFNGG+LG+  KIL  K  V   + S EY S+ 
Sbjct: 65  LLQFILALIATPLAKLDAFLLKLFNFISFNGGLLGILSKILRGKGFVKVKEDSSEYTSVV 124

Query: 126 AFTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWN 185
            F DWRRDLDSSI+   +FRYYSALTVMA KISYE +P+VQSVVN RW+M LLGYF+FWN
Sbjct: 125 GFADWRRDLDSSIKSEKSFRYYSALTVMATKISYESKPFVQSVVNDRWKMKLLGYFNFWN 184

Query: 186 DFQRKATTQAFMFETSATDPNVTVVAFRGTSPLDTYDWQVDADFSWYEIEGVGRMHSGFM 245
           DFQ KATTQAF FE +A DPNV +VAFRGTSPLD YDWQVD D SWYEIEGVGR+HSGFM
Sbjct: 185 DFQGKATTQAFTFENTAIDPNVVIVAFRGTSPLDAYDWQVDVDLSWYEIEGVGRIHSGFM 244

Query: 246 KALGLQKATGWPKELPGPQQHDFAYYTLRQKLRDIATANHNARFIITGHSLGGALATVFV 305
           KALGLQKATGWPKELP  Q H+FAYYTLR++LRDIA AN  ARFI TGHSLGGALAT+FV
Sbjct: 245 KALGLQKATGWPKELPKTQTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFV 304

Query: 306 TILALHHESSILEKLEAVYTYGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPRVP 365
           T+L+LH ES+ILEKL++VYTYGQPRVG++ FAKFMV+ VQKYGFKY+RYVYS DLVPRVP
Sbjct: 305 TVLSLHDESTILEKLDSVYTYGQPRVGDQQFAKFMVNCVQKYGFKYHRYVYSYDLVPRVP 364

Query: 366 FDAIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPRFLSAWWEVIRSWIIPFV 425
            DA+ FKY+HFG+ VYFNSLY+GRI+KEQP +NY SL W IP++LSAWWE+IRS+IIPFV
Sbjct: 365 SDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNRNYFSLLWVIPKYLSAWWELIRSFIIPFV 424

Query: 426 RGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDIEP 485
           +GFDYYE+LLM+GAR+VGLL+PG++AHFP+NYVN+TRLGKL +    ++E+P L  +IE 
Sbjct: 425 KGFDYYESLLMKGARLVGLLIPGLSAHFPLNYVNVTRLGKLNV--PDEVEDPFLGYEIED 483

Query: 486 DY 488
           D+
Sbjct: 485 DF 483

BLAST of Lag0036958 vs. ExPASy TrEMBL
Match: A0A6J1CEI9 (uncharacterized protein LOC111010491 OS=Momordica charantia OX=3673 GN=LOC111010491 PE=4 SV=1)

HSP 1 Score: 703.0 bits (1813), Expect = 8.9e-199
Identity = 338/485 (69.69%), Postives = 407/485 (83.92%), Query Frame = 0

Query: 3   SDQEHAFSRNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKGIEDS-LRKFTDRLSI 62
           + +EH FS+NYLILKP+NAN+ DLFLFLLPFG+R+R +FIDC +G EDS    FT+R  I
Sbjct: 2   ASKEHEFSKNYLILKPQNANIVDLFLFLLPFGFRKRTKFIDCPEGKEDSYTSSFTNRWLI 61

Query: 63  VISMVLQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLVWPDKSSPEY 122
            IS+VLQIILLA ATPLAKLD+FLLN+FNFISFNGG+LGL  +IL  + LV PD++S EY
Sbjct: 62  FISIVLQIILLAIATPLAKLDAFLLNVFNFISFNGGILGLLSRILKGESLVKPDENSAEY 121

Query: 123 MSLAAFTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYF 182
            S+  F DWRRDLD SI+  + FRYY  LTVMAA++SYE +P+V SVVN RW+M LLG+F
Sbjct: 122 TSVVGFADWRRDLDKSIKAEEIFRYYGGLTVMAAQLSYESKPFVLSVVNDRWKMKLLGHF 181

Query: 183 DFWNDFQRKATTQAFMFETSATDPNVTVVAFRGTSPLDTYDWQVDADFSWYEIEGVGRMH 242
           +FWNDFQ KATTQAFMF+ +A DPNVTVVAFRGTSPLD YDWQVDADFSWY+IEGVGR+H
Sbjct: 182 NFWNDFQGKATTQAFMFQNTAADPNVTVVAFRGTSPLDAYDWQVDADFSWYDIEGVGRIH 241

Query: 243 SGFMKALGLQKATGWPKELPGPQQHDFAYYTLRQKLRDIATANHNARFIITGHSLGGALA 302
           SGFMKALGLQKATGWP+EL    +H FAYYTLRQKLRDIA +N NA+FIITGHSLGGALA
Sbjct: 242 SGFMKALGLQKATGWPEELV-KSKHHFAYYTLRQKLRDIAKSNDNAKFIITGHSLGGALA 301

Query: 303 TVFVTILALHHESSILEKLEAVYTYGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLV 362
            +FVT+L+LH +S++LEKL+AVYTY QPRVG+R FA+FMV+TV+KYGFKY+RYVYSSDLV
Sbjct: 302 ALFVTVLSLHRDSAVLEKLQAVYTYSQPRVGDRRFAEFMVNTVKKYGFKYHRYVYSSDLV 361

Query: 363 PRVPFDAIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPRFLSAWWEVIRSWI 422
           PRVPFD + FKY+HFG+ +YFNSLY+GRI+KE+P KNY SL W +P++L+AWWE+IRS+I
Sbjct: 362 PRVPFDGVIFKYKHFGRCIYFNSLYKGRIVKEEPNKNYFSLIWVMPKYLNAWWELIRSFI 421

Query: 423 IPFVRGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILED 482
           +P V+GFDY+E+LLM+GARVVGL +PG+AAH P +YVN  RLGK     Q        ED
Sbjct: 422 VPLVKGFDYHESLLMKGARVVGLFIPGLAAHIPTDYVNSIRLGKFNPPPQE------FED 479

Query: 483 DIEPD 487
            IE D
Sbjct: 482 SIEAD 479

BLAST of Lag0036958 vs. ExPASy TrEMBL
Match: A0A6J1J3Y9 (uncharacterized protein LOC111481121 OS=Cucurbita maxima OX=3661 GN=LOC111481121 PE=4 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 1.3e-197
Identity = 341/480 (71.04%), Postives = 408/480 (85.00%), Query Frame = 0

Query: 9   FSRNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKGIEDSLRKFTDRLSIVISMVLQ 68
           FS+NY+ILKP +A + DLFLFLLPFG  R+ +FIDC +G E S   FTDR  I IS++LQ
Sbjct: 8   FSKNYMILKPNDAKLLDLFLFLLPFG-LRKTKFIDCPEGNEASYSNFTDRWFIFISILLQ 67

Query: 69  IILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLVWPDKSSPEYMSLAAFT 128
           IIL A ATPL+KLDSFL+NLFNFISFNGG+LGL  KIL  K LV P++ S +Y S+  F 
Sbjct: 68  IILAAAATPLSKLDSFLVNLFNFISFNGGILGLLSKILKGKGLVKPNEDSSDYTSVLGFA 127

Query: 129 DWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWNDFQ 188
           DWR DLDSSI   DTFRYY  LT+MAAKI+YE +P+VQSVVN RW+M LLG+F+FWNDFQ
Sbjct: 128 DWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFNFWNDFQ 187

Query: 189 RKATTQAFMFETSAT-DPNVTVVAFRGTSPLDTYDWQVDADFSWYEIEGVGRMHSGFMKA 248
            KATTQAFMF+ +AT DPN+ V+AFRGTSPLDT DWQV+ADFSWY+IEG+GR+HSGFMKA
Sbjct: 188 GKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTDDWQVNADFSWYDIEGLGRIHSGFMKA 247

Query: 249 LGLQKATGWPKELPGPQQHDFAYYTLRQKLRDIATANHNARFIITGHSLGGALATVFVTI 308
           LGLQKATGWPKEL  P QHDFAYY+LRQKLRDIA AN  ARFIITGHSLGGALAT+FVT+
Sbjct: 248 LGLQKATGWPKELTKP-QHDFAYYSLRQKLRDIAKANAKARFIITGHSLGGALATLFVTL 307

Query: 309 LALHHESSILEKLEAVYTYGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPRVPFD 368
           L+LH E++ILEKL+ VYTYGQPRVG+R FA+FMV+TVQKYGFKYYRYVYSSDLVPR+P D
Sbjct: 308 LSLHDETTILEKLQGVYTYGQPRVGDRRFAEFMVNTVQKYGFKYYRYVYSSDLVPRIPSD 367

Query: 369 AIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPRFLSAWWEVIRSWIIPFVRG 428
            + FKY+HFG+ +YFNSLY+GRI+KEQP KNY SL W IP++LSAWWE++RS+I P + G
Sbjct: 368 GVIFKYKHFGRSIYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELVRSFITPLMWG 427

Query: 429 FDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKLRMARQRQLEEPILEDDIEPDY 488
           FD+YE+LLM  AR+VGL++PG+AAHFP+NYVN  RLGKL++    ++E+PILEDDIE +Y
Sbjct: 428 FDHYESLLMSAARMVGLIIPGLAAHFPLNYVNSIRLGKLKV--PDEIEDPILEDDIEGEY 483

BLAST of Lag0036958 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 409.8 bits (1052), Expect = 3.0e-114
Identity = 213/460 (46.30%), Postives = 298/460 (64.78%), Query Frame = 0

Query: 9   FSRNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKG-IEDSLRKFTDRLSIVISMVL 68
           F  +Y ++ P  A+  DL L L         RFID     ++   R F  R  + +++ L
Sbjct: 8   FCNSYFLVDPTKASFLDLLLLLFSSN-LTSARFIDSPPDTLKGFRRSFASRWILALAIFL 67

Query: 69  QIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLVWPDKSSPEYMSLAAF 128
           Q +L+  + P A +   L    N ++ NGG   L   +++  KLV PDKSS  Y S    
Sbjct: 68  QKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMS-GKLVKPDKSSATYTSFIGC 127

Query: 129 TDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWNDF 188
           +D R +LD  I    +  Y S L++MA+KISYE +PY+ SVV + W+M+L+G +DF+N F
Sbjct: 128 SDRRIELDEKINVG-SIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNAF 187

Query: 189 QRKATTQAFMFETSATDPNVTVVAFRGTSPLDTYDWQVDADFSWYEIEGVGRMHSGFMKA 248
           Q    TQAF+F+TS+T+P++ VV+FRGT P +  DW  D D SWYE++ VG++H+GF +A
Sbjct: 188 QESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRA 247

Query: 249 LGLQKATGWPKELPGPQQHDFAYYTLRQKLRDIATANHNARFIITGHSLGGALATVFVTI 308
           LGLQK  GWPKE      H +AYYT+RQ LRD    N N ++I+TGHSLGGALA +F  I
Sbjct: 248 LGLQK-DGWPKENIS-LLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPAI 307

Query: 309 LALHHESSILEKLEAVYTYGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPRVPF- 368
           LA+H E  +L+KLE +YT+GQPRVG+ DF +FM   V+K+G +Y R+VY++D+VPRVPF 
Sbjct: 308 LAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPFD 367

Query: 369 DAIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPRFLSAWWEVIRSWIIPFVR 428
           D   F Y+H+G    FNSLY+G++ ++ P  NY +L W IP+ L+  WE IRS+I+ F +
Sbjct: 368 DKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLLTGLWEFIRSFILQFWK 427

Query: 429 GFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKL 467
           G +Y E  LMR  RVVG++ PG + HFP +YVN TRLG L
Sbjct: 428 GDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGL 462

BLAST of Lag0036958 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 404.4 bits (1038), Expect = 1.3e-112
Identity = 196/461 (42.52%), Postives = 302/461 (65.51%), Query Frame = 0

Query: 10  SRNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKGIEDSLRKFTDRLSIVISMVLQI 69
           ++NY +L P  A V DL   L       R+      + IED L +F  R  I +S+V+Q 
Sbjct: 4   TKNYFVLDPREATVSDLIHLLFSSDLGDRKFIHSSEERIEDDLSRFRRRWIIFVSIVIQK 63

Query: 70  ILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLVWPDKSSPEYMSLAAFTD 129
           +++ F  PL  +   L    N +S NGG L +    L    ++WP+K+S  + SL    D
Sbjct: 64  LIILFKKPLYFVGFSLACWLNLLSSNGGFL-MILPNLFKGNIIWPEKASATFASLNGNLD 123

Query: 130 WRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWNDFQR 189
            R +L+  +E     RY + L++MA+K+SYE   +V SV+++ W+M+LLG++  WN +Q+
Sbjct: 124 RRVELNPKVERGSK-RYKAMLSIMASKLSYENINFVSSVLHNHWKMDLLGFYSCWNGYQK 183

Query: 190 KATTQAFMFETSATDPNVTVVAFRGTSPLDTYDWQVDADFSWYEIEGVGRMHSGFMKALG 249
           + +T+  + + ++TDPN+ +V+FRGT P D  DW  D D SWYE++ VG++H GFMKALG
Sbjct: 184 QRSTEVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKNVGKIHGGFMKALG 243

Query: 250 LQKATGWPKELPGPQQHD----FAYYTLRQKLRDIATANHNARFIITGHSLGGALATVFV 309
           LQK  GWPKE+   +  +    +AYYT+R+ L++I   N  ++FI+TGHSLGGALA +F 
Sbjct: 244 LQK-EGWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFILTGHSLGGALAILFT 303

Query: 310 TILALHHESSILEKLEAVYTYGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPRVP 369
            +L +H E  +LE+LE VYT+GQPRVG+ +F  FM D+++K+  KY RYVY +D+VPR+P
Sbjct: 304 AVLVMHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKDSLKKFDVKYERYVYCNDMVPRLP 363

Query: 370 FDAIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPRFLSAWWEVIRSWIIPFV 429
           FD     ++HFG  +Y++S Y+G++ +E+P KNY +L W +P+ ++A WE+IRS+++P+ 
Sbjct: 364 FDDKTLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLVWVLPKIMNALWELIRSFVMPYW 423

Query: 430 RGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLGKL 467
           +G ++ E   +R  RVV LL+PG+ AHFP  Y+N+T LG L
Sbjct: 424 KGGEFREGWFLRCFRVVALLIPGLPAHFPNEYINVTLLGDL 461

BLAST of Lag0036958 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 361.3 bits (926), Expect = 1.2e-99
Identity = 188/461 (40.78%), Postives = 292/461 (63.34%), Query Frame = 0

Query: 11  RNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCS-KGIEDSLRKFTDRLSIVISMVLQI 70
           +NY +L P  A V DL + LL +     R+F+D S + +E+ L +F  R  I +S+V+Q 
Sbjct: 5   KNYFVLDPREATVTDL-MRLLFYSDLENRKFVDTSVENLENRLCEFRGRWIIFVSIVVQK 64

Query: 71  ILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLVWPDKSSPEYMSLAAFTD 130
           +L+    PL+ L   L    N  S NGG   +F   L   + +WP+K+S  + S+    D
Sbjct: 65  LLIILRKPLSFLGFALGFWLNLPSSNGGFFKIFLN-LVKGRFIWPEKTSATFASINGNLD 124

Query: 131 WRRD--LDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWNDF 190
            + +  L  SI+  D  RY   L++MA+K++YE + +++SV+   W+M+LLG++   NDF
Sbjct: 125 QKVELGLGRSIKIGDE-RYKPLLSIMASKLAYENEHFIRSVLQDHWQMDLLGFYSCPNDF 184

Query: 191 QRKATTQAFMFETSATDPNVTVVAFRGTSPLDTYDWQVDADFSWYEIEGVGRMHSGFMKA 250
            +  +T+  +   +  +PN+ VV+FRGT P +  DW  D D SW+ +  VG++H GFMKA
Sbjct: 185 DQTRSTEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVMNVGKIHGGFMKA 244

Query: 251 LGLQKATGWPKELPGPQQHD----FAYYTLRQKLRDIATANHNARFIITGHSLGGALATV 310
           LGL K  GW +E+   Q  +     AYYT+ ++L+++   N  ++FI++GHSLGGALA +
Sbjct: 245 LGLPK-EGWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFILSGHSLGGALAIL 304

Query: 311 FVTILALHHESSILEKLEAVYTYGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPR 370
           F  +L +H E  +LE+LE VYT+GQPRVG+ DF  +M D ++++  KY RYVY +D+VPR
Sbjct: 305 FTAVLIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKDKLKEFDVKYKRYVYCNDMVPR 364

Query: 371 VPFDAIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPRFLSAWWEVIRSWIIP 430
           +PFD     ++HFG  +Y +S Y+G++ +E+P KNY ++FW IP+ ++A WE+IRS+II 
Sbjct: 365 LPFDDKTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFWVIPKIINAVWELIRSFIIS 424

Query: 431 FVRGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRLG 465
             +G +Y E  L+   R+V LL+PG+ AHFP  YVN+  LG
Sbjct: 425 CWQGREYREGWLLTCFRLVALLIPGLPAHFPNEYVNVALLG 461

BLAST of Lag0036958 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 354.4 bits (908), Expect = 1.5e-97
Identity = 194/471 (41.19%), Postives = 284/471 (60.30%), Query Frame = 0

Query: 1   MGSDQEHAFSRNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKGIEDSLRKFTDRLS 60
           M SD +    R YLIL+PE    ++L + LL  G   + R +D S+  E S   F  R  
Sbjct: 2   MNSDDDDE-PRGYLILRPEELRPWEL-VRLLFSGDIEKPRSVDSSETEEHS---FRHRWL 61

Query: 61  IVISMVLQIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLVWPDKSSPE 120
           I +S+VL  +L  F+  LA + S L    NF+S N    GLF +     ++V P ++S  
Sbjct: 62  IFVSLVLLKLLRFFSKLLALVGSALEFSLNFLS-NNSFSGLFLR----GEVVMPQRTSEN 121

Query: 121 YMSLAAFTDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGY 180
           Y S     D R  LD ++   D  +YY+AL++MA+KI+YE    ++ VV + W M  LG 
Sbjct: 122 YQSFIGHLDTRVSLDMTLNREDGEKYYAALSIMASKIAYENAARIKHVVENHWNMKYLGL 181

Query: 181 FDFWNDFQRKATTQAFMFETSAT------DPNVTVVAFRGTSPLDTYDWQVDADFSWYEI 240
            D+WN++Q K TTQAF+  T  T           VVAFRGT   ++ DW  D D +W+E+
Sbjct: 182 VDYWNEYQEKETTQAFIMSTDETTTRSNGQETTVVVAFRGTELFNSEDWCSDFDITWFEL 241

Query: 241 EGVGRMHSGFMKALGLQKATGWPKE-LPGP-QQHDFAYYTLRQKLRDIATANHNARFIIT 300
             +G +H GFMKALGLQ    WPKE L  P ++   AYY++R  L+ +   N N +F++T
Sbjct: 242 PNIGNIHGGFMKALGLQNNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNKNTKFVLT 301

Query: 301 GHSLGGALATVFVTILALHHESSILEKLEAVYTYGQPRVGNRDFAKFMVDTVQKYGFKYY 360
           GHSLGGALA +F  +L +HHE+ +LE+++ VYTYGQPRVG+  F +FM   ++KY  KYY
Sbjct: 302 GHSLGGALAILFTAVLVIHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEKYNIKYY 361

Query: 361 RYVYSSDLVPRVPFDAIFFKYRHFGKVVYFNSLYRGRILKEQPYKNYISLFWAIPRFLSA 420
           R+VY++D+VPR+P+D     ++HFG  +Y++  Y+ ++++EQ  +N+  L   I    SA
Sbjct: 362 RFVYNNDIVPRLPYDDKDLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLRGIIKMMWSA 421

Query: 421 WWEVIRSWIIPFVRGFDYYETLLMRGARVVGLLVPGVAAHFPVNYVNITRL 464
             E IRS+ I   +G +Y E  L++G R +G++VPGV+ H P +YVN TRL
Sbjct: 422 ILEFIRSFTIVAEKGSEYSEGWLLKGGRALGIIVPGVSNHTPQDYVNATRL 462

BLAST of Lag0036958 vs. TAIR 10
Match: AT1G45201.2 (triacylglycerol lipase-like 1 )

HSP 1 Score: 332.4 bits (851), Expect = 6.1e-91
Identity = 175/384 (45.57%), Postives = 247/384 (64.32%), Query Frame = 0

Query: 9   FSRNYLILKPENANVFDLFLFLLPFGWRRRRRFIDCSKG-IEDSLRKFTDRLSIVISMVL 68
           F  +Y ++ P  A+  DL L L         RFID     ++   R F  R  + +++ L
Sbjct: 8   FCNSYFLVDPTKASFLDLLLLLFSSN-LTSARFIDSPPDTLKGFRRSFASRWILALAIFL 67

Query: 69  QIILLAFATPLAKLDSFLLNLFNFISFNGGVLGLFFKILTVKKLVWPDKSSPEYMSLAAF 128
           Q +L+  + P A +   L    N ++ NGG   L   +++  KLV PDKSS  Y S    
Sbjct: 68  QKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMS-GKLVKPDKSSATYTSFIGC 127

Query: 129 TDWRRDLDSSIEPSDTFRYYSALTVMAAKISYEFQPYVQSVVNHRWRMNLLGYFDFWNDF 188
           +D R +LD  I    +  Y S L++MA+KISYE +PY+ SVV + W+M+L+G +DF+N F
Sbjct: 128 SDRRIELDEKINVG-SIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNAF 187

Query: 189 QRKATTQAFMFETSATDPNVTVVAFRGTSPLDTYDWQVDADFSWYEIEGVGRMHSGFMKA 248
           Q    TQAF+F+TS+T+P++ VV+FRGT P +  DW  D D SWYE++ VG++H+GF +A
Sbjct: 188 QESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRA 247

Query: 249 LGLQKATGWPKELPGPQQHDFAYYTLRQKLRDIATANHNARFIITGHSLGGALATVFVTI 308
           LGLQK  GWPKE      H +AYYT+RQ LRD    N N ++I+TGHSLGGALA +F  I
Sbjct: 248 LGLQK-DGWPKENIS-LLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPAI 307

Query: 309 LALHHESSILEKLEAVYTYGQPRVGNRDFAKFMVDTVQKYGFKYYRYVYSSDLVPRVPF- 368
           LA+H E  +L+KLE +YT+GQPRVG+ DF +FM   V+K+G +Y R+VY++D+VPRVPF 
Sbjct: 308 LAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPFD 367

Query: 369 DAIFFKYRHFGKVVYFNSLYRGRI 391
           D   F Y+H+G    FNSLY+G++
Sbjct: 368 DKYLFSYKHYGPCNSFNSLYKGKV 386