Lsi09G005860 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G005860
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr09: 6309483 .. 6311024 (+)
RNA-Seq ExpressionLsi09G005860
SyntenyLsi09G005860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATGCTCAAGAGGCTAACTCAAATCCCTCAAATACCCATTTCTTCCCATTCGTTTCTGATTCTTGCAGCTCCTTTTTGTACTCATCTTCATCTTCATCCGTCTTCACAACCCACAACTGAAATCGAAAGAATCGCCAAAATTATCAACGACCACCCATTTCCCGACCACCCCCTTCAACCCATTCTTCTTCACCACATTCCATCTCCTCTTCCTTCAAACACTTTCCTCAATGACGTTCTTGGCCGCCTCTTTGCAGCCCATTCCAATGGCCTCAAAGCCTTAGAGTTTTTCAAATTCTGCCTTCACCATTCTCAAGCTTCTTCCCCAACTCCAGACGCTTTCGAGAAGACGCTTCACATTCTCGCCAGGATGAGGTATTTTGATCAATCATGGGAATTGATGCGTGAGATTGGACGAACTCACCCTTTTTTGCTTACTCTCAAGTCCATGAGCATCTTGCTTTCGAAGATCGCGAAGTTTCAATCATTCGAAGAAACTATCGAAGCGTTTCAGAGAATGGAGAATGAAGTGTTTGTTGGAAGGAAATTTGGTACTGAGGAATTTAATGTACTCCTTCGAGCTTTTTGCACTCAGAGACAGATGAAAGAGGCGCGATCAGTGTTCCAAAAGATGTATTCTCGATTTCCCCCAAATACTAAAACCATGAATCTCTTGCTTTTAGGTTTTAAGGAATCGACCGATGTTACTGCTGTTGAGCTTTTTTATCATGAGATGATTAAGAGAGGTTTCAAGCCAAATGCTGTGACTTATAGCATTAGAATTGATGCATATTGCAAAAGAGGTTGTTTTGTGGATGGTTTGAGAGTTCTTAAAGAAATGGAGAGGGCAAAATTTGAGCCAACATTGGAAACAATCACTACTCTGATCCATGGAGCAGGGTTAACAAAGGATAAAACCAAAGCACGCCAACTGTTTGATGAAATTACTCTGAGAAACTTATGTCCTGACATTGGGGCTTATAATGCTTTGATAAGTTCCTTGATTAGGTCTGATGATGTAAAGTGTGCAGCAGCTTTAATGGAAGATATGGAAGCTAAACACATTGGACATGACAGTCTGACCTATCATATGATGTTCTCAGGCCTGATGCGATTGGAGGATGTTGGTGGGTTTTATGAGTTGTATAGCAAGATGGTACGAAGAAATTTCGTGCCTAAAACACGAACGGTAATCATGATAATGAAGTTTTTCTGCGAAAATCGCCGAGTTGATTTGGGTTTGGAATTGTGGGGTTATCTGGTAGAGAAGGGTTACTGTCCTCATAGTCATGTTTTGGATCTGTTGGTGACAGGATTATGTGCCCGTGGAATGGTCCTTCAAGCATTTGAGTGCTCCAAACAAATGTTGGAGAGAGGGAGACAAATGAGTGAAGCAGCATTTCTGATCATGAAGAGATCTCTTTTGCAGGCACATGCAACAGACAATTATGGGGAGCTTGAACAGTTGAGGAAGAAGCTTAAAACTGTTTTGCCCCCACCAAACCAGCTTTCATCTGAGATTTCAGCATCCTCATAG

mRNA sequence

ATGGCGATGCTCAAGAGGCTAACTCAAATCCCTCAAATACCCATTTCTTCCCATTCGTTTCTGATTCTTGCAGCTCCTTTTTGTACTCATCTTCATCTTCATCCGTCTTCACAACCCACAACTGAAATCGAAAGAATCGCCAAAATTATCAACGACCACCCATTTCCCGACCACCCCCTTCAACCCATTCTTCTTCACCACATTCCATCTCCTCTTCCTTCAAACACTTTCCTCAATGACGTTCTTGGCCGCCTCTTTGCAGCCCATTCCAATGGCCTCAAAGCCTTAGAGTTTTTCAAATTCTGCCTTCACCATTCTCAAGCTTCTTCCCCAACTCCAGACGCTTTCGAGAAGACGCTTCACATTCTCGCCAGGATGAGGTATTTTGATCAATCATGGGAATTGATGCGTGAGATTGGACGAACTCACCCTTTTTTGCTTACTCTCAAGTCCATGAGCATCTTGCTTTCGAAGATCGCGAAGTTTCAATCATTCGAAGAAACTATCGAAGCGTTTCAGAGAATGGAGAATGAAGTGTTTGTTGGAAGGAAATTTGGTACTGAGGAATTTAATGTACTCCTTCGAGCTTTTTGCACTCAGAGACAGATGAAAGAGGCGCGATCAGTGTTCCAAAAGATGTATTCTCGATTTCCCCCAAATACTAAAACCATGAATCTCTTGCTTTTAGGTTTTAAGGAATCGACCGATGTTACTGCTGTTGAGCTTTTTTATCATGAGATGATTAAGAGAGGTTTCAAGCCAAATGCTGTGACTTATAGCATTAGAATTGATGCATATTGCAAAAGAGGTTGTTTTGTGGATGGTTTGAGAGTTCTTAAAGAAATGGAGAGGGCAAAATTTGAGCCAACATTGGAAACAATCACTACTCTGATCCATGGAGCAGGGTTAACAAAGGATAAAACCAAAGCACGCCAACTGTTTGATGAAATTACTCTGAGAAACTTATGTCCTGACATTGGGGCTTATAATGCTTTGATAAGTTCCTTGATTAGGTCTGATGATGTAAAGTGTGCAGCAGCTTTAATGGAAGATATGGAAGCTAAACACATTGGACATGACAGTCTGACCTATCATATGATGTTCTCAGGCCTGATGCGATTGGAGGATGTTGGTGGGTTTTATGAGTTGTATAGCAAGATGGTACGAAGAAATTTCGTGCCTAAAACACGAACGGTAATCATGATAATGAAGTTTTTCTGCGAAAATCGCCGAGTTGATTTGGGTTTGGAATTGTGGGGTTATCTGGTAGAGAAGGGTTACTGTCCTCATAGTCATGTTTTGGATCTGTTGGTGACAGGATTATGTGCCCGTGGAATGGTCCTTCAAGCATTTGAGTGCTCCAAACAAATGTTGGAGAGAGGGAGACAAATGAGTGAAGCAGCATTTCTGATCATGAAGAGATCTCTTTTGCAGGCACATGCAACAGACAATTATGGGGAGCTTGAACAGTTGAGGAAGAAGCTTAAAACTGTTTTGCCCCCACCAAACCAGCTTTCATCTGAGATTTCAGCATCCTCATAG

Coding sequence (CDS)

ATGGCGATGCTCAAGAGGCTAACTCAAATCCCTCAAATACCCATTTCTTCCCATTCGTTTCTGATTCTTGCAGCTCCTTTTTGTACTCATCTTCATCTTCATCCGTCTTCACAACCCACAACTGAAATCGAAAGAATCGCCAAAATTATCAACGACCACCCATTTCCCGACCACCCCCTTCAACCCATTCTTCTTCACCACATTCCATCTCCTCTTCCTTCAAACACTTTCCTCAATGACGTTCTTGGCCGCCTCTTTGCAGCCCATTCCAATGGCCTCAAAGCCTTAGAGTTTTTCAAATTCTGCCTTCACCATTCTCAAGCTTCTTCCCCAACTCCAGACGCTTTCGAGAAGACGCTTCACATTCTCGCCAGGATGAGGTATTTTGATCAATCATGGGAATTGATGCGTGAGATTGGACGAACTCACCCTTTTTTGCTTACTCTCAAGTCCATGAGCATCTTGCTTTCGAAGATCGCGAAGTTTCAATCATTCGAAGAAACTATCGAAGCGTTTCAGAGAATGGAGAATGAAGTGTTTGTTGGAAGGAAATTTGGTACTGAGGAATTTAATGTACTCCTTCGAGCTTTTTGCACTCAGAGACAGATGAAAGAGGCGCGATCAGTGTTCCAAAAGATGTATTCTCGATTTCCCCCAAATACTAAAACCATGAATCTCTTGCTTTTAGGTTTTAAGGAATCGACCGATGTTACTGCTGTTGAGCTTTTTTATCATGAGATGATTAAGAGAGGTTTCAAGCCAAATGCTGTGACTTATAGCATTAGAATTGATGCATATTGCAAAAGAGGTTGTTTTGTGGATGGTTTGAGAGTTCTTAAAGAAATGGAGAGGGCAAAATTTGAGCCAACATTGGAAACAATCACTACTCTGATCCATGGAGCAGGGTTAACAAAGGATAAAACCAAAGCACGCCAACTGTTTGATGAAATTACTCTGAGAAACTTATGTCCTGACATTGGGGCTTATAATGCTTTGATAAGTTCCTTGATTAGGTCTGATGATGTAAAGTGTGCAGCAGCTTTAATGGAAGATATGGAAGCTAAACACATTGGACATGACAGTCTGACCTATCATATGATGTTCTCAGGCCTGATGCGATTGGAGGATGTTGGTGGGTTTTATGAGTTGTATAGCAAGATGGTACGAAGAAATTTCGTGCCTAAAACACGAACGGTAATCATGATAATGAAGTTTTTCTGCGAAAATCGCCGAGTTGATTTGGGTTTGGAATTGTGGGGTTATCTGGTAGAGAAGGGTTACTGTCCTCATAGTCATGTTTTGGATCTGTTGGTGACAGGATTATGTGCCCGTGGAATGGTCCTTCAAGCATTTGAGTGCTCCAAACAAATGTTGGAGAGAGGGAGACAAATGAGTGAAGCAGCATTTCTGATCATGAAGAGATCTCTTTTGCAGGCACATGCAACAGACAATTATGGGGAGCTTGAACAGTTGAGGAAGAAGCTTAAAACTGTTTTGCCCCCACCAAACCAGCTTTCATCTGAGATTTCAGCATCCTCATAG

Protein sequence

MAMLKRLTQIPQIPISSHSFLILAAPFCTHLHLHPSSQPTTEIERIAKIINDHPFPDHPLQPILLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFEKTLHILARMRYFDQSWELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVFVGRKFGTEEFNVLLRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAVELFYHEMIKRGFKPNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHGAGLTKDKTKARQLFDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHDSLTYHMMFSGLMRLEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWGYLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAHATDNYGELEQLRKKLKTVLPPPNQLSSEISASS
Homology
BLAST of Lsi09G005860 vs. ExPASy Swiss-Prot
Match: Q9M2C8 (Pentatricopeptide repeat-containing protein At3g61360 OS=Arabidopsis thaliana OX=3702 GN=At3g61360 PE=2 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 1.2e-158
Identity = 286/491 (58.25%), Postives = 368/491 (74.95%), Query Frame = 0

Query: 15  ISSHSFLILAAP-FCTHLHLHPSSQPTTEIERIAKIINDHPFPDHPLQPILLHHIPSPLP 74
           ISS S+  L AP   +   L  +S   TEIERI  IIN HPFP+HP+QPIL  HIP    
Sbjct: 5   ISSDSYRRLLAPVLSSSSSLSSTSINRTEIERITIIINGHPFPNHPIQPILAKHIPLSSL 64

Query: 75  SNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFEKTLHILARMRYFDQSW 134
           S  F+++VLGRLFAAHSNGLKALEFFK+ L  S+ SSPT D+FEKTLHILARMRYFDQ+W
Sbjct: 65  SPEFVSEVLGRLFAAHSNGLKALEFFKYSLKSSK-SSPTSDSFEKTLHILARMRYFDQAW 124

Query: 135 ELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVFVGRKFGTEEFNVL 194
            LM E+ + +P LL+ KSMSILL KIAKF S+EET+EAF +ME E+F  +KFG +EFN+L
Sbjct: 125 ALMAEVRKDYPNLLSFKSMSILLCKIAKFGSYEETLEAFVKMEKEIF-RKKFGVDEFNIL 184

Query: 195 LRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAVELFYHEMIKRGFK 254
           LRAFCT+R+MKEARS+F+K++SRF P+ KTMN+LLLGFKE+ DVTA ELFYHEM+KRGFK
Sbjct: 185 LRAFCTEREMKEARSIFEKLHSRFNPDVKTMNILLLGFKEAGDVTATELFYHEMVKRGFK 244

Query: 255 PNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHGAGLTKDKTKARQL 314
           PN+VTY IRID +CK+  F + LR+ ++M+R  F+ T++ +TTLIHG+G+ ++K KARQL
Sbjct: 245 PNSVTYGIRIDGFCKKRNFGEALRLFEDMDRLDFDITVQILTTLIHGSGVARNKIKARQL 304

Query: 315 FDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHDSLTYHMMFSGLMR 374
           FDEI+ R L PD GAYNAL+SSL++  DV  A  +M++ME K I  DS+T+H MF G+M+
Sbjct: 305 FDEISKRGLTPDCGAYNALMSSLMKCGDVSGAIKVMKEMEEKGIEPDSVTFHSMFIGMMK 364

Query: 375 LEDVG--GFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWGYLVEKGYCPHS 434
            ++ G  G  E Y KM  R+ VPKT T++M+MK FC N  V+LGL+LW Y++EKGYCPH 
Sbjct: 365 SKEFGFNGVCEYYQKMKERSLVPKTPTIVMLMKLFCHNGEVNLGLDLWKYMLEKGYCPHG 424

Query: 435 HVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAHATDNYGELEQL 494
           H L+LL T LCAR     AFECS Q +ERGR +SE  + +++ SL   +      EL++ 
Sbjct: 425 HALELLTTALCARRRANDAFECSWQTVERGRCVSEPVYRMLETSLSSNNELKKLEELKEE 484

Query: 495 RKKLKTVLPPP 503
            +KL + LPPP
Sbjct: 485 IQKLHSFLPPP 493

BLAST of Lsi09G005860 vs. ExPASy Swiss-Prot
Match: Q9FZ19 (Putative pentatricopeptide repeat-containing protein At1g02420 OS=Arabidopsis thaliana OX=3702 GN=At1g02420 PE=3 SV=2)

HSP 1 Score: 230.3 bits (586), Expect = 4.8e-59
Identity = 138/432 (31.94%), Postives = 231/432 (53.47%), Query Frame = 0

Query: 74  SNTFLNDVLGRLFAAHSNGLKALEFFKFC-----LHHSQASSPTPDAFEKTLHILARMRY 133
           S   ++ VL R+  +H N ++ LEF+++       +HS  S  T       L+IL R R 
Sbjct: 70  SKDLIDRVLKRVRFSHGNPIQTLEFYRYASAIRGFYHSSFSLDT------MLYILGRNRK 129

Query: 134 FDQSWELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVFVGRKFGTE 193
           FDQ WEL+ E  R    L++ ++M ++L ++AK  S  +T+E+F + +    V   F T 
Sbjct: 130 FDQIWELLIETKRKDRSLISPRTMQVVLGRVAKLCSVRQTVESFWKFKR--LVPDFFDTA 189

Query: 194 EFNVLLRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAVELFYHEMI 253
            FN LLR  C ++ M +AR+V+  +  +F P+ +T N+LL G+K S +    E F+ EM 
Sbjct: 190 CFNALLRTLCQEKSMTDARNVYHSLKHQFQPDLQTFNILLSGWKSSEE---AEAFFEEMK 249

Query: 254 KRGFKPNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHGAGLTKDKT 313
            +G KP+ VTY+  ID YCK        +++ +M   +  P + T TT+I G GL     
Sbjct: 250 GKGLKPDVVTYNSLIDVYCKDREIEKAYKLIDKMREEEETPDVITYTTVIGGLGLIGQPD 309

Query: 314 KARQLFDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHDSLTYHMMF 373
           KAR++  E+      PD+ AYNA I +   +  +  A  L+++M  K +  ++ TY++ F
Sbjct: 310 KAREVLKEMKEYGCYPDVAAYNAAIRNFCIARRLGDADKLVDEMVKKGLSPNATTYNLFF 369

Query: 374 SGLMRLEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWGYLVEKGYC 433
             L    D+G  +ELY +M+    +P T++ + ++K F  + +VD+ + LW  +V KG+ 
Sbjct: 370 RVLSLANDLGRSWELYVRMLGNECLPNTQSCMFLIKMFKRHEKVDMAMRLWEDMVVKGFG 429

Query: 434 PHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAHATDNYGEL 493
            +S V D+L+  LC    V +A +C  +M+E+G + S  +F  +K  +  A+  D    L
Sbjct: 430 SYSLVSDVLLDLLCDLAKVEEAEKCLLEMVEKGHRPSNVSFKRIKLLMELANKHDEVNNL 489

Query: 494 EQLRKKLKTVLP 501
            Q      T +P
Sbjct: 490 IQKMAIFSTEIP 490

BLAST of Lsi09G005860 vs. ExPASy Swiss-Prot
Match: Q9C9A2 (Pentatricopeptide repeat-containing protein At1g71060, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71060 PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 5.9e-41
Identity = 130/501 (25.95%), Postives = 240/501 (47.90%), Query Frame = 0

Query: 6   RLTQIPQIPISSHSFLILAAPFCTHLHLHPSSQPT--------TEIERIAKIINDHPFPD 65
           R++      + S  ++  A+ F  +   H SS  T         + ERI KI+    F D
Sbjct: 21  RISSSSSPSLESIPWIHKASNFTLYGSFHASSVETQVSANDASQDAERICKILT--KFTD 80

Query: 66  HPLQPILLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFE 125
             ++ +L  +  S   S   + +VL +L  A   G+ AL  FK+   + +    T   + 
Sbjct: 81  SKVETLL--NEASVKLSPALIEEVLKKLSNA---GVLALSVFKWA-ENQKGFKHTTSNYN 140

Query: 126 KTLHILARMRYFDQSWELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMEN 185
             +  L +++ F   W L+ ++      LL+ ++ +++  + A+ +  +E I AF +ME 
Sbjct: 141 ALIESLGKIKQFKLIWSLVDDMKAKK--LLSKETFALISRRYARARKVKEAIGAFHKMEE 200

Query: 186 EVFVGRKFGTEEFNVLLRAFCTQRQMKEARSVFQKM-YSRFPPNTKTMNLLLLGFKESTD 245
               G K  + +FN +L      R + +A+ VF KM   RF P+ K+  +LL G+ +  +
Sbjct: 201 ---FGFKMESSDFNRMLDTLSKSRNVGDAQKVFDKMKKKRFEPDIKSYTILLEGWGQELN 260

Query: 246 VTAVELFYHEMIKRGFKPNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITT 305
           +  V+    EM   GF+P+ V Y I I+A+CK   + + +R   EME+   +P+     +
Sbjct: 261 LLRVDEVNREMKDEGFEPDVVAYGIIINAHCKAKKYEEAIRFFNEMEQRNCKPSPHIFCS 320

Query: 306 LIHGAGLTKDKTKARQLFDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKH 365
           LI+G G  K    A + F+         +   YNAL+ +   S  ++ A   +++M  K 
Sbjct: 321 LINGLGSEKKLNDALEFFERSKSSGFPLEAPTYNALVGAYCWSQRMEDAYKTVDEMRLKG 380

Query: 366 IGHDSLTYHMMFSGLMRLEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGL 425
           +G ++ TY ++   L+R++     YE+Y  M   +  P   T  ++++ FC   R+D+ +
Sbjct: 381 VGPNARTYDIILHHLIRMQRSKEAYEVYQTM---SCEPTVSTYEIMVRMFCNKERLDMAI 440

Query: 426 ELWGYLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSL 485
           ++W  +  KG  P  H+   L+T LC    + +A E   +ML+ G +     F  +K++L
Sbjct: 441 KIWDEMKGKGVLPGMHMFSSLITALCHENKLDEACEYFNEMLDVGIRPPGHMFSRLKQTL 500

Query: 486 L----QAHATDNYGELEQLRK 494
           L    +   TD   ++++LRK
Sbjct: 501 LDEGRKDKVTDLVVKMDRLRK 505

BLAST of Lsi09G005860 vs. ExPASy Swiss-Prot
Match: Q9FVX2 (Pentatricopeptide repeat-containing protein At1g77360, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g77360 PE=2 SV=2)

HSP 1 Score: 166.8 bits (421), Expect = 6.5e-40
Identity = 107/429 (24.94%), Postives = 213/429 (49.65%), Query Frame = 0

Query: 74  SNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFEKTLHILARMRYFDQSW 133
           S   + DVL R     + GL    FF++     +    +  A+   +   A++R +   W
Sbjct: 99  SQEVVEDVLNRF---RNAGLLTYRFFQWS-EKQRHYEHSVRAYHMMIESTAKIRQYKLMW 158

Query: 134 ELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVFVGRKFGTEEFNVL 193
           +L+  + +    +L +++  I++ K A+ Q  +E I AF  ME             FN L
Sbjct: 159 DLINAMRKKK--MLNVETFCIVMRKYARAQKVDEAIYAFNVMEKYDLPPNLVA---FNGL 218

Query: 194 LRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAVELFYHEMIKRGFK 253
           L A C  + +++A+ VF+ M  RF P++KT ++LL G+ +  ++      + EMI  G  
Sbjct: 219 LSALCKSKNVRKAQEVFENMRDRFTPDSKTYSILLEGWGKEPNLPKAREVFREMIDAGCH 278

Query: 254 PNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHGAGLTKDKTKARQL 313
           P+ VTYSI +D  CK G   + L +++ M+ +  +PT    + L+H  G      +A   
Sbjct: 279 PDIVTYSIMVDILCKAGRVDEALGIVRSMDPSICKPTTFIYSVLVHTYGTENRLEEAVDT 338

Query: 314 FDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHDSLTYHMMFSGLMR 373
           F E+    +  D+  +N+LI +  +++ +K    ++++M++K +  +S + +++   L+ 
Sbjct: 339 FLEMERSGMKADVAVFNSLIGAFCKANRMKNVYRVLKEMKSKGVTPNSKSCNIILRHLIE 398

Query: 374 LEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWGYLVEKGYCPHSHV 433
             +    ++++ KM+ +   P   T  M++K FCE + ++   ++W Y+ +KG  P  H 
Sbjct: 399 RGEKDEAFDVFRKMI-KVCEPDADTYTMVIKMFCEKKEMETADKVWKYMRKKGVFPSMHT 458

Query: 434 LDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAHATDNYGELEQLRK 493
             +L+ GLC      +A    ++M+E G + S   F  +++ L++    D    L+ L +
Sbjct: 459 FSVLINGLCEERTTQKACVLLEEMIEMGIRPSGVTFGRLRQLLIKEERED---VLKFLNE 514

Query: 494 KLKTVLPPP 503
           K+  ++  P
Sbjct: 519 KMNVLVNEP 514

BLAST of Lsi09G005860 vs. ExPASy Swiss-Prot
Match: Q9S7R4 (Pentatricopeptide repeat-containing protein At1g74900, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OTP43 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 1.1e-34
Identity = 107/426 (25.12%), Postives = 197/426 (46.24%), Query Frame = 0

Query: 39  PTTEIERIAKIINDHPFPDHPLQPILLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEF 98
           P  +   IAK+I   P   H     LL    +P   N  +N VL RL+   ++G KAL+F
Sbjct: 21  PPADSAAIAKLILSSPNTTHQDDQFLLSTKTTPWTPN-LVNSVLKRLW---NHGPKALQF 80

Query: 99  FKFCLHHSQASSPTPDAFEKTLHILARMRYFDQSWELMREIGRTHPFLLTLKSMSILLSK 158
           F F  +H +       +F+  + I AR+      W L+  + R+     + K+ +I+  +
Sbjct: 81  FHFLDNHHREYVHDASSFDLAIDIAARLHLHPTVWSLIHRM-RSLRIGPSPKTFAIVAER 140

Query: 159 IAKFQSFEETIEAFQRMENEVFVGRKFGTEEFNVLLRAFCTQRQMKEARSVFQKMYSRFP 218
            A     ++ ++ F  M      G       FN +L   C  +++++A  +F+ +  RF 
Sbjct: 141 YASAGKPDKAVKLFLNMHEH---GCFQDLASFNTILDVLCKSKRVEKAYELFRALRGRFS 200

Query: 219 PNTKTMNLLLLGFKESTDVTAVELFYHEMIKRGFKPNAVTYSIRIDAYCKRGCFVDGLRV 278
            +T T N++L G+              EM++RG  PN  TY+  +  + + G        
Sbjct: 201 VDTVTYNVILNGWCLIKRTPKALEVLKEMVERGINPNLTTYNTMLKGFFRAGQIRHAWEF 260

Query: 279 LKEMERAKFEPTLETITTLIHGAGLTKDKTKARQLFDEITLRNLCPDIGAYNALISSLIR 338
             EM++   E  + T TT++HG G+  +  +AR +FDE+    + P +  YNA+I  L +
Sbjct: 261 FLEMKKRDCEIDVVTYTTVVHGFGVAGEIKRARNVFDEMIREGVLPSVATYNAMIQVLCK 320

Query: 339 SDDVKCAAALMEDMEAKHIGHDSLTYHMMFSGLMRLEDVGGFYELYSKMVRRNFVPKTRT 398
            D+V+ A  + E+M  +    +  TY+++  GL    +     EL  +M      P  +T
Sbjct: 321 KDNVENAVVMFEEMVRRGYEPNVTTYNVLIRGLFHAGEFSRGEELMQRMENEGCEPNFQT 380

Query: 399 VIMIMKFFCENRRVDLGLELWGYLVEKGYCPHSHVLDLLVTGLCARGM---VLQAFECSK 458
             M+++++ E   V+  L L+  +      P+    ++L++G+  R     ++ A +   
Sbjct: 381 YNMMIRYYSECSEVEKALGLFEKMGSGDCLPNLDTYNILISGMFVRKRSEDMVVAGKLLL 438

Query: 459 QMLERG 462
           +M+ERG
Sbjct: 441 EMVERG 438

BLAST of Lsi09G005860 vs. ExPASy TrEMBL
Match: A0A5A7UJI2 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001000 PE=4 SV=1)

HSP 1 Score: 906.4 bits (2341), Expect = 5.6e-260
Identity = 456/512 (89.06%), Postives = 475/512 (92.77%), Query Frame = 0

Query: 1   MAMLKRLTQIPQIPISSHSFLILAAPFCTHLHLHPSSQPTTEIERIAKIINDHPFPDHPL 60
           MA+L +LTQIPQ PISSHSFLI AA +CTHLH     + TTEIERIAKIINDHPFPD PL
Sbjct: 1   MAILMKLTQIPQKPISSHSFLIYAASYCTHLH-----KSTTEIERIAKIINDHPFPDQPL 60

Query: 61  QPILLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFEKTL 120
            P  LH IPSPLPSNTFLNDVLG LFAAHSNGLKALEFFKFCLHHSQA SPTPDAFEKTL
Sbjct: 61  HPTFLHLIPSPLPSNTFLNDVLGHLFAAHSNGLKALEFFKFCLHHSQA-SPTPDAFEKTL 120

Query: 121 HILARMRYFDQSWELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVF 180
           HILARMRYFDQSWELMREI +THPFLLTLKSMSILLS+IAKF+SFEETIEAFQRMENEVF
Sbjct: 121 HILARMRYFDQSWELMREIRQTHPFLLTLKSMSILLSRIAKFRSFEETIEAFQRMENEVF 180

Query: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAV 240
           VGRKFGTEEFNVLLRAFCTQRQMKEARSVF KMYSRFPPN KT+NLLLLGFKES+D+T+V
Sbjct: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFHKMYSRFPPNIKTINLLLLGFKESSDITSV 240

Query: 241 ELFYHEMIKRGFKPNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHG 300
           ELFYHEMIKRGFKPNAVTYSIRIDAYCK+GCFVDGLRV KEMERAK EPTLETITTLIHG
Sbjct: 241 ELFYHEMIKRGFKPNAVTYSIRIDAYCKKGCFVDGLRVFKEMERAKLEPTLETITTLIHG 300

Query: 301 AGLTKDKTKARQLFDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHD 360
           AG+ KDKTKARQLFDEI LRNLCPDIGAYNALISSLIRS DVK AAALMEDMEAKHIGHD
Sbjct: 301 AGIVKDKTKARQLFDEIPLRNLCPDIGAYNALISSLIRSGDVKSAAALMEDMEAKHIGHD 360

Query: 361 SLTYHMMFSGLMRLEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWG 420
           S+TYHMMF GL+RLEDVGGF ELYSKMVR+NFVPKTRT +MIMKFFCENRRVDL L  W 
Sbjct: 361 SVTYHMMFLGLIRLEDVGGFRELYSKMVRQNFVPKTRTTVMIMKFFCENRRVDLALGFWA 420

Query: 421 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAH 480
           YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIM+RSLL+A 
Sbjct: 421 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMERSLLKAR 480

Query: 481 ATDNYGELEQLRKKLKTVLPPPNQLSSEISAS 513
           ATD YGELEQLRKKLKTVLPPPNQL SEI AS
Sbjct: 481 ATDKYGELEQLRKKLKTVLPPPNQLLSEIPAS 506

BLAST of Lsi09G005860 vs. ExPASy TrEMBL
Match: A0A5D3BMK0 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1728G00390 PE=4 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 9.5e-260
Identity = 456/512 (89.06%), Postives = 475/512 (92.77%), Query Frame = 0

Query: 1   MAMLKRLTQIPQIPISSHSFLILAAPFCTHLHLHPSSQPTTEIERIAKIINDHPFPDHPL 60
           MA+L +LTQIPQ PISSHSFLI AA +CTHLH     + TTEIERIAKIINDHPFPD PL
Sbjct: 1   MAILMKLTQIPQKPISSHSFLIYAASYCTHLH-----KSTTEIERIAKIINDHPFPDQPL 60

Query: 61  QPILLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFEKTL 120
            P LLH IPSPLPSNTFLNDVLG LFAAHSNGLKALEFFKFCLHHSQA SPTPDAFEKTL
Sbjct: 61  HPTLLHLIPSPLPSNTFLNDVLGHLFAAHSNGLKALEFFKFCLHHSQA-SPTPDAFEKTL 120

Query: 121 HILARMRYFDQSWELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVF 180
           HILARMRYFDQSWELMREI +THPFLLTLKSMSILLS+IAKF+SFEETIEAFQRMENEVF
Sbjct: 121 HILARMRYFDQSWELMREIRQTHPFLLTLKSMSILLSRIAKFRSFEETIEAFQRMENEVF 180

Query: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAV 240
           VGRKFGTEEFNVLLRAFCTQRQMKEARSVF KMYSRFPPN KT+NLLLLGFKES+D+T+V
Sbjct: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFHKMYSRFPPNIKTINLLLLGFKESSDITSV 240

Query: 241 ELFYHEMIKRGFKPNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHG 300
           ELFYHEMIKRGFKPNAVTYSIRIDAYCK+GCFVDGLRV KEMERAK EPTLETITTLIHG
Sbjct: 241 ELFYHEMIKRGFKPNAVTYSIRIDAYCKKGCFVDGLRVFKEMERAKLEPTLETITTLIHG 300

Query: 301 AGLTKDKTKARQLFDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHD 360
           AG+ KDKTKARQLFDEI LRNLCPDIGAYNALISSLIRS DVK AAALMEDMEAKHIGHD
Sbjct: 301 AGIVKDKTKARQLFDEIPLRNLCPDIGAYNALISSLIRSGDVKSAAALMEDMEAKHIGHD 360

Query: 361 SLTYHMMFSGLMRLEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWG 420
           S+TYHMMF GL+RLEDVGGF ELYSKMVR+NFVPKTRT + IMKFFCENRRVDL L  W 
Sbjct: 361 SVTYHMMFLGLIRLEDVGGFRELYSKMVRQNFVPKTRTTVTIMKFFCENRRVDLALGFWA 420

Query: 421 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAH 480
           YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIM+RSLL+A 
Sbjct: 421 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMERSLLKAR 480

Query: 481 ATDNYGELEQLRKKLKTVLPPPNQLSSEISAS 513
           ATD YGELEQLRKKLKTVLPPPNQL SEI AS
Sbjct: 481 ATDKYGELEQLRKKLKTVLPPPNQLLSEIPAS 506

BLAST of Lsi09G005860 vs. ExPASy TrEMBL
Match: A0A1S4DS46 (pentatricopeptide repeat-containing protein At3g61360 OS=Cucumis melo OX=3656 GN=LOC103504627 PE=4 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 9.5e-260
Identity = 456/512 (89.06%), Postives = 475/512 (92.77%), Query Frame = 0

Query: 1   MAMLKRLTQIPQIPISSHSFLILAAPFCTHLHLHPSSQPTTEIERIAKIINDHPFPDHPL 60
           MA+L +LTQIPQ PISSHSFLI AA +CTHLH     + TTEIERIAKIINDHPFPD PL
Sbjct: 5   MAILMKLTQIPQKPISSHSFLIYAASYCTHLH-----KSTTEIERIAKIINDHPFPDQPL 64

Query: 61  QPILLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFEKTL 120
            P LLH IPSPLPSNTFLNDVLG LFAAHSNGLKALEFFKFCLHHSQA SPTPDAFEKTL
Sbjct: 65  HPTLLHLIPSPLPSNTFLNDVLGHLFAAHSNGLKALEFFKFCLHHSQA-SPTPDAFEKTL 124

Query: 121 HILARMRYFDQSWELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVF 180
           HILARMRYFDQSWELMREI +THPFLLTLKSMSILLS+IAKF+SFEETIEAFQRMENEVF
Sbjct: 125 HILARMRYFDQSWELMREIRQTHPFLLTLKSMSILLSRIAKFRSFEETIEAFQRMENEVF 184

Query: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAV 240
           VGRKFGTEEFNVLLRAFCTQRQMKEARSVF KMYSRFPPN KT+NLLLLGFKES+D+T+V
Sbjct: 185 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFHKMYSRFPPNIKTINLLLLGFKESSDITSV 244

Query: 241 ELFYHEMIKRGFKPNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHG 300
           ELFYHEMIKRGFKPNAVTYSIRIDAYCK+GCFVDGLRV KEMERAK EPTLETITTLIHG
Sbjct: 245 ELFYHEMIKRGFKPNAVTYSIRIDAYCKKGCFVDGLRVFKEMERAKLEPTLETITTLIHG 304

Query: 301 AGLTKDKTKARQLFDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHD 360
           AG+ KDKTKARQLFDEI LRNLCPDIGAYNALISSLIRS DVK AAALMEDMEAKHIGHD
Sbjct: 305 AGIVKDKTKARQLFDEIPLRNLCPDIGAYNALISSLIRSGDVKSAAALMEDMEAKHIGHD 364

Query: 361 SLTYHMMFSGLMRLEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWG 420
           S+TYHMMF GL+RLEDVGGF ELYSKMVR+NFVPKTRT + IMKFFCENRRVDL L  W 
Sbjct: 365 SVTYHMMFLGLIRLEDVGGFRELYSKMVRQNFVPKTRTTVTIMKFFCENRRVDLALGFWA 424

Query: 421 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAH 480
           YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIM+RSLL+A 
Sbjct: 425 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMERSLLKAR 484

Query: 481 ATDNYGELEQLRKKLKTVLPPPNQLSSEISAS 513
           ATD YGELEQLRKKLKTVLPPPNQL SEI AS
Sbjct: 485 ATDKYGELEQLRKKLKTVLPPPNQLLSEIPAS 510

BLAST of Lsi09G005860 vs. ExPASy TrEMBL
Match: A0A6J1EM60 (pentatricopeptide repeat-containing protein At3g61360 OS=Cucurbita moschata OX=3662 GN=LOC111435788 PE=4 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 8.3e-256
Identity = 451/513 (87.91%), Postives = 474/513 (92.40%), Query Frame = 0

Query: 1   MAMLKRLTQIPQIPISSHSFLILAAPFCTHLHLHPSSQPTTEIERIAKIINDHPFPDHPL 60
           M +  RLTQI QI I  HSFLI AAPF T  HLH SS+P  EIERI KIINDHPFPD PL
Sbjct: 1   MVIPTRLTQISQIRIFFHSFLIPAAPFST--HLHQSSEPGGEIERITKIINDHPFPDQPL 60

Query: 61  QPILLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFEKTL 120
           +P LLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQA SPTPDAFEKTL
Sbjct: 61  RPTLLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQA-SPTPDAFEKTL 120

Query: 121 HILARMRYFDQSWELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVF 180
           HILARMRYFDQSWELMREI RTHPFLLTLKSMSILL+KIAKFQSFEETIEAF+RMENEVF
Sbjct: 121 HILARMRYFDQSWELMREIQRTHPFLLTLKSMSILLTKIAKFQSFEETIEAFRRMENEVF 180

Query: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAV 240
           VGRKFGTEEFNVLLRAFCTQRQMKEARSVF KMYSRFPPNTKTMNLLLLGFKES++VTAV
Sbjct: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFLKMYSRFPPNTKTMNLLLLGFKESSNVTAV 240

Query: 241 ELFYHEMIKRGFKPNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHG 300
           ELFYHEMI+RGFKP+AVTYSIR+DAYCKRGCFVDGLRV +EM+RAKFEPTLETITTLIHG
Sbjct: 241 ELFYHEMIRRGFKPDAVTYSIRMDAYCKRGCFVDGLRVFEEMKRAKFEPTLETITTLIHG 300

Query: 301 AGLTKDKTKARQLFDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHD 360
           AG+ KD  KARQLFDE+ LRNLCPDIGAYNALISSLIRSDDV  AAALMEDMEAKHIGHD
Sbjct: 301 AGVAKDIAKARQLFDEMPLRNLCPDIGAYNALISSLIRSDDVNSAAALMEDMEAKHIGHD 360

Query: 361 SLTYHMMFSGLMRLEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWG 420
           S+TYHMMF GLM+LEDV GFYELY KM+RRNFVPKTRTV+MIMKFFCENRRVDLGL+LWG
Sbjct: 361 SMTYHMMFVGLMKLEDVHGFYELYRKMIRRNFVPKTRTVVMIMKFFCENRRVDLGLDLWG 420

Query: 421 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAH 480
           YLVEKGYCPHSHVLD+LVTGLCARGMV +AFECSKQMLERGRQMS+AAFLIM+RSLLQA 
Sbjct: 421 YLVEKGYCPHSHVLDVLVTGLCARGMVHEAFECSKQMLERGRQMSDAAFLIMERSLLQAD 480

Query: 481 ATDNYGELEQLRKKLKTVLPPPNQLSSEISASS 514
           A D  GELEQLRKKLKTVLPPP QL  EI ASS
Sbjct: 481 AKDILGELEQLRKKLKTVLPPPKQLPYEIPASS 510

BLAST of Lsi09G005860 vs. ExPASy TrEMBL
Match: A0A0A0KRS8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G173440 PE=4 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 1.7e-253
Identity = 444/501 (88.62%), Postives = 467/501 (93.21%), Query Frame = 0

Query: 1   MAMLKRLTQIPQIPISSHSFLILAAPFCTHLHLHPSSQPTTEIERIAKIINDHPFPDHPL 60
           MA+L +LT IPQ PISSHSFLILAA +CT  +LHPSS+ TTEIERIAKIINDHPFPD PL
Sbjct: 1   MAVLMKLTLIPQKPISSHSFLILAASYCT--NLHPSSKSTTEIERIAKIINDHPFPDQPL 60

Query: 61  QPILLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFEKTL 120
            P LLH IPSPLPSNTFLNDVLG+LFAAHSNGLKALEFFKFCLHHSQA  PT DAFEKTL
Sbjct: 61  HPTLLHLIPSPLPSNTFLNDVLGQLFAAHSNGLKALEFFKFCLHHSQA-PPTSDAFEKTL 120

Query: 121 HILARMRYFDQSWELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVF 180
           HIL+RMRYFDQSWELMREI +THPFLLTLKSMSILLS+IAKF SFEETIEAFQRMENEVF
Sbjct: 121 HILSRMRYFDQSWELMREIRQTHPFLLTLKSMSILLSRIAKFLSFEETIEAFQRMENEVF 180

Query: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAV 240
           VGRKFGTEEFNVLLRAFCTQRQMKEARSVF KMYSRFPPN KT+NLLLLGFKES+D+T+V
Sbjct: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFHKMYSRFPPNIKTINLLLLGFKESSDITSV 240

Query: 241 ELFYHEMIKRGFKPNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHG 300
           ELFYHEMIKRGFKPNAVTYSIRIDAYCK+GCFVDGLRV KEMERAK EPTLETITTLIHG
Sbjct: 241 ELFYHEMIKRGFKPNAVTYSIRIDAYCKKGCFVDGLRVFKEMERAKCEPTLETITTLIHG 300

Query: 301 AGLTKDKTKARQLFDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHD 360
           AG+ KDKTKARQLFDEI LRNLCPDIGAYNALISSLIRS DVK AA++MEDMEAKHI HD
Sbjct: 301 AGIVKDKTKARQLFDEIPLRNLCPDIGAYNALISSLIRSGDVKSAASVMEDMEAKHIEHD 360

Query: 361 SLTYHMMFSGLMRLEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWG 420
           S+TYHMMFSGL+RLEDVGGFYELY KMV RNFVPKTRT +MIMKFFCENRRVDLGL  W 
Sbjct: 361 SVTYHMMFSGLIRLEDVGGFYELYIKMVGRNFVPKTRTAVMIMKFFCENRRVDLGLGFWA 420

Query: 421 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAH 480
           YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIM+R LL+AH
Sbjct: 421 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMERCLLKAH 480

Query: 481 ATDNYGELEQLRKKLKTVLPP 502
           ATD Y ELE+LRKKLKTVLPP
Sbjct: 481 ATDKYEELERLRKKLKTVLPP 498

BLAST of Lsi09G005860 vs. NCBI nr
Match: XP_038876095.1 (pentatricopeptide repeat-containing protein At3g61360 [Benincasa hispida] >XP_038876096.1 pentatricopeptide repeat-containing protein At3g61360 [Benincasa hispida])

HSP 1 Score: 948.7 bits (2451), Expect = 2.0e-272
Identity = 470/513 (91.62%), Postives = 489/513 (95.32%), Query Frame = 0

Query: 1   MAMLKRLTQIPQIPISSHSFLILAAPFCTHLHLHPSSQPTTEIERIAKIINDHPFPDHPL 60
           MA+L+RLTQIPQ+PIS HSFLI AAPFCTH+HLHPSSQP TE+ERIAKIINDHPFPDHPL
Sbjct: 1   MAILQRLTQIPQLPISFHSFLIYAAPFCTHIHLHPSSQPKTEVERIAKIINDHPFPDHPL 60

Query: 61  QPILLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFEKTL 120
            P LLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALE FKFCLHHSQ  SPTPDAFEKTL
Sbjct: 61  HPTLLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALELFKFCLHHSQ-PSPTPDAFEKTL 120

Query: 121 HILARMRYFDQSWELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVF 180
           HILARMRYFDQSWELMREI RTHPFLLTLKSMSIL+SKIAKFQSFEETIEAF RMENEVF
Sbjct: 121 HILARMRYFDQSWELMREIQRTHPFLLTLKSMSILISKIAKFQSFEETIEAFHRMENEVF 180

Query: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAV 240
           VGRKFG EEFNVLLRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKES+DVTAV
Sbjct: 181 VGRKFGIEEFNVLLRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESSDVTAV 240

Query: 241 ELFYHEMIKRGFKPNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHG 300
           ELFYHEMI+RGFKPNAVTYSIRIDAYCKRG FVDGLRV +EMERAK EPTLETITTLIHG
Sbjct: 241 ELFYHEMIRRGFKPNAVTYSIRIDAYCKRGYFVDGLRVFEEMERAKLEPTLETITTLIHG 300

Query: 301 AGLTKDKTKARQLFDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHD 360
           AG+ KDKTKARQLFDEITLRNLCPDIGAYNALISSLIRS+DVK AAALMEDMEAKHIGHD
Sbjct: 301 AGVAKDKTKARQLFDEITLRNLCPDIGAYNALISSLIRSNDVKSAAALMEDMEAKHIGHD 360

Query: 361 SLTYHMMFSGLMRLEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWG 420
           S+TYHM+FSGLMRLEDVGGFYELYSKM+R+NFVPKTRTV+MIMKF CENRRVDLGL+ WG
Sbjct: 361 SMTYHMLFSGLMRLEDVGGFYELYSKMIRQNFVPKTRTVVMIMKFLCENRRVDLGLDFWG 420

Query: 421 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAH 480
           YLVEKGYCPHSHVLDLLVTGLCARGMVLQA ECSKQMLERGRQMSEAAFLIMKR+LLQAH
Sbjct: 421 YLVEKGYCPHSHVLDLLVTGLCARGMVLQASECSKQMLERGRQMSEAAFLIMKRTLLQAH 480

Query: 481 ATDNYGELEQLRKKLKTVLPPPNQLSSEISASS 514
           ATD YGELEQLR KL+TVLPPP QLS EI ASS
Sbjct: 481 ATDKYGELEQLRNKLQTVLPPPKQLSFEIPASS 512

BLAST of Lsi09G005860 vs. NCBI nr
Match: KAA0055168.1 (pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 906.4 bits (2341), Expect = 1.1e-259
Identity = 456/512 (89.06%), Postives = 475/512 (92.77%), Query Frame = 0

Query: 1   MAMLKRLTQIPQIPISSHSFLILAAPFCTHLHLHPSSQPTTEIERIAKIINDHPFPDHPL 60
           MA+L +LTQIPQ PISSHSFLI AA +CTHLH     + TTEIERIAKIINDHPFPD PL
Sbjct: 1   MAILMKLTQIPQKPISSHSFLIYAASYCTHLH-----KSTTEIERIAKIINDHPFPDQPL 60

Query: 61  QPILLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFEKTL 120
            P  LH IPSPLPSNTFLNDVLG LFAAHSNGLKALEFFKFCLHHSQA SPTPDAFEKTL
Sbjct: 61  HPTFLHLIPSPLPSNTFLNDVLGHLFAAHSNGLKALEFFKFCLHHSQA-SPTPDAFEKTL 120

Query: 121 HILARMRYFDQSWELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVF 180
           HILARMRYFDQSWELMREI +THPFLLTLKSMSILLS+IAKF+SFEETIEAFQRMENEVF
Sbjct: 121 HILARMRYFDQSWELMREIRQTHPFLLTLKSMSILLSRIAKFRSFEETIEAFQRMENEVF 180

Query: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAV 240
           VGRKFGTEEFNVLLRAFCTQRQMKEARSVF KMYSRFPPN KT+NLLLLGFKES+D+T+V
Sbjct: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFHKMYSRFPPNIKTINLLLLGFKESSDITSV 240

Query: 241 ELFYHEMIKRGFKPNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHG 300
           ELFYHEMIKRGFKPNAVTYSIRIDAYCK+GCFVDGLRV KEMERAK EPTLETITTLIHG
Sbjct: 241 ELFYHEMIKRGFKPNAVTYSIRIDAYCKKGCFVDGLRVFKEMERAKLEPTLETITTLIHG 300

Query: 301 AGLTKDKTKARQLFDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHD 360
           AG+ KDKTKARQLFDEI LRNLCPDIGAYNALISSLIRS DVK AAALMEDMEAKHIGHD
Sbjct: 301 AGIVKDKTKARQLFDEIPLRNLCPDIGAYNALISSLIRSGDVKSAAALMEDMEAKHIGHD 360

Query: 361 SLTYHMMFSGLMRLEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWG 420
           S+TYHMMF GL+RLEDVGGF ELYSKMVR+NFVPKTRT +MIMKFFCENRRVDL L  W 
Sbjct: 361 SVTYHMMFLGLIRLEDVGGFRELYSKMVRQNFVPKTRTTVMIMKFFCENRRVDLALGFWA 420

Query: 421 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAH 480
           YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIM+RSLL+A 
Sbjct: 421 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMERSLLKAR 480

Query: 481 ATDNYGELEQLRKKLKTVLPPPNQLSSEISAS 513
           ATD YGELEQLRKKLKTVLPPPNQL SEI AS
Sbjct: 481 ATDKYGELEQLRKKLKTVLPPPNQLLSEIPAS 506

BLAST of Lsi09G005860 vs. NCBI nr
Match: TYK00295.1 (pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 905.6 bits (2339), Expect = 2.0e-259
Identity = 456/512 (89.06%), Postives = 475/512 (92.77%), Query Frame = 0

Query: 1   MAMLKRLTQIPQIPISSHSFLILAAPFCTHLHLHPSSQPTTEIERIAKIINDHPFPDHPL 60
           MA+L +LTQIPQ PISSHSFLI AA +CTHLH     + TTEIERIAKIINDHPFPD PL
Sbjct: 1   MAILMKLTQIPQKPISSHSFLIYAASYCTHLH-----KSTTEIERIAKIINDHPFPDQPL 60

Query: 61  QPILLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFEKTL 120
            P LLH IPSPLPSNTFLNDVLG LFAAHSNGLKALEFFKFCLHHSQA SPTPDAFEKTL
Sbjct: 61  HPTLLHLIPSPLPSNTFLNDVLGHLFAAHSNGLKALEFFKFCLHHSQA-SPTPDAFEKTL 120

Query: 121 HILARMRYFDQSWELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVF 180
           HILARMRYFDQSWELMREI +THPFLLTLKSMSILLS+IAKF+SFEETIEAFQRMENEVF
Sbjct: 121 HILARMRYFDQSWELMREIRQTHPFLLTLKSMSILLSRIAKFRSFEETIEAFQRMENEVF 180

Query: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAV 240
           VGRKFGTEEFNVLLRAFCTQRQMKEARSVF KMYSRFPPN KT+NLLLLGFKES+D+T+V
Sbjct: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFHKMYSRFPPNIKTINLLLLGFKESSDITSV 240

Query: 241 ELFYHEMIKRGFKPNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHG 300
           ELFYHEMIKRGFKPNAVTYSIRIDAYCK+GCFVDGLRV KEMERAK EPTLETITTLIHG
Sbjct: 241 ELFYHEMIKRGFKPNAVTYSIRIDAYCKKGCFVDGLRVFKEMERAKLEPTLETITTLIHG 300

Query: 301 AGLTKDKTKARQLFDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHD 360
           AG+ KDKTKARQLFDEI LRNLCPDIGAYNALISSLIRS DVK AAALMEDMEAKHIGHD
Sbjct: 301 AGIVKDKTKARQLFDEIPLRNLCPDIGAYNALISSLIRSGDVKSAAALMEDMEAKHIGHD 360

Query: 361 SLTYHMMFSGLMRLEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWG 420
           S+TYHMMF GL+RLEDVGGF ELYSKMVR+NFVPKTRT + IMKFFCENRRVDL L  W 
Sbjct: 361 SVTYHMMFLGLIRLEDVGGFRELYSKMVRQNFVPKTRTTVTIMKFFCENRRVDLALGFWA 420

Query: 421 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAH 480
           YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIM+RSLL+A 
Sbjct: 421 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMERSLLKAR 480

Query: 481 ATDNYGELEQLRKKLKTVLPPPNQLSSEISAS 513
           ATD YGELEQLRKKLKTVLPPPNQL SEI AS
Sbjct: 481 ATDKYGELEQLRKKLKTVLPPPNQLLSEIPAS 506

BLAST of Lsi09G005860 vs. NCBI nr
Match: XP_016898809.1 (PREDICTED: pentatricopeptide repeat-containing protein At3g61360 [Cucumis melo])

HSP 1 Score: 905.6 bits (2339), Expect = 2.0e-259
Identity = 456/512 (89.06%), Postives = 475/512 (92.77%), Query Frame = 0

Query: 1   MAMLKRLTQIPQIPISSHSFLILAAPFCTHLHLHPSSQPTTEIERIAKIINDHPFPDHPL 60
           MA+L +LTQIPQ PISSHSFLI AA +CTHLH     + TTEIERIAKIINDHPFPD PL
Sbjct: 5   MAILMKLTQIPQKPISSHSFLIYAASYCTHLH-----KSTTEIERIAKIINDHPFPDQPL 64

Query: 61  QPILLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFEKTL 120
            P LLH IPSPLPSNTFLNDVLG LFAAHSNGLKALEFFKFCLHHSQA SPTPDAFEKTL
Sbjct: 65  HPTLLHLIPSPLPSNTFLNDVLGHLFAAHSNGLKALEFFKFCLHHSQA-SPTPDAFEKTL 124

Query: 121 HILARMRYFDQSWELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVF 180
           HILARMRYFDQSWELMREI +THPFLLTLKSMSILLS+IAKF+SFEETIEAFQRMENEVF
Sbjct: 125 HILARMRYFDQSWELMREIRQTHPFLLTLKSMSILLSRIAKFRSFEETIEAFQRMENEVF 184

Query: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAV 240
           VGRKFGTEEFNVLLRAFCTQRQMKEARSVF KMYSRFPPN KT+NLLLLGFKES+D+T+V
Sbjct: 185 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFHKMYSRFPPNIKTINLLLLGFKESSDITSV 244

Query: 241 ELFYHEMIKRGFKPNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHG 300
           ELFYHEMIKRGFKPNAVTYSIRIDAYCK+GCFVDGLRV KEMERAK EPTLETITTLIHG
Sbjct: 245 ELFYHEMIKRGFKPNAVTYSIRIDAYCKKGCFVDGLRVFKEMERAKLEPTLETITTLIHG 304

Query: 301 AGLTKDKTKARQLFDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHD 360
           AG+ KDKTKARQLFDEI LRNLCPDIGAYNALISSLIRS DVK AAALMEDMEAKHIGHD
Sbjct: 305 AGIVKDKTKARQLFDEIPLRNLCPDIGAYNALISSLIRSGDVKSAAALMEDMEAKHIGHD 364

Query: 361 SLTYHMMFSGLMRLEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWG 420
           S+TYHMMF GL+RLEDVGGF ELYSKMVR+NFVPKTRT + IMKFFCENRRVDL L  W 
Sbjct: 365 SVTYHMMFLGLIRLEDVGGFRELYSKMVRQNFVPKTRTTVTIMKFFCENRRVDLALGFWA 424

Query: 421 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAH 480
           YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIM+RSLL+A 
Sbjct: 425 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMERSLLKAR 484

Query: 481 ATDNYGELEQLRKKLKTVLPPPNQLSSEISAS 513
           ATD YGELEQLRKKLKTVLPPPNQL SEI AS
Sbjct: 485 ATDKYGELEQLRKKLKTVLPPPNQLLSEIPAS 510

BLAST of Lsi09G005860 vs. NCBI nr
Match: XP_022929103.1 (pentatricopeptide repeat-containing protein At3g61360 [Cucurbita moschata] >XP_022929104.1 pentatricopeptide repeat-containing protein At3g61360 [Cucurbita moschata] >XP_022929105.1 pentatricopeptide repeat-containing protein At3g61360 [Cucurbita moschata] >XP_022929106.1 pentatricopeptide repeat-containing protein At3g61360 [Cucurbita moschata])

HSP 1 Score: 892.5 bits (2305), Expect = 1.7e-255
Identity = 451/513 (87.91%), Postives = 474/513 (92.40%), Query Frame = 0

Query: 1   MAMLKRLTQIPQIPISSHSFLILAAPFCTHLHLHPSSQPTTEIERIAKIINDHPFPDHPL 60
           M +  RLTQI QI I  HSFLI AAPF T  HLH SS+P  EIERI KIINDHPFPD PL
Sbjct: 1   MVIPTRLTQISQIRIFFHSFLIPAAPFST--HLHQSSEPGGEIERITKIINDHPFPDQPL 60

Query: 61  QPILLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFEKTL 120
           +P LLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQA SPTPDAFEKTL
Sbjct: 61  RPTLLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQA-SPTPDAFEKTL 120

Query: 121 HILARMRYFDQSWELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVF 180
           HILARMRYFDQSWELMREI RTHPFLLTLKSMSILL+KIAKFQSFEETIEAF+RMENEVF
Sbjct: 121 HILARMRYFDQSWELMREIQRTHPFLLTLKSMSILLTKIAKFQSFEETIEAFRRMENEVF 180

Query: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAV 240
           VGRKFGTEEFNVLLRAFCTQRQMKEARSVF KMYSRFPPNTKTMNLLLLGFKES++VTAV
Sbjct: 181 VGRKFGTEEFNVLLRAFCTQRQMKEARSVFLKMYSRFPPNTKTMNLLLLGFKESSNVTAV 240

Query: 241 ELFYHEMIKRGFKPNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHG 300
           ELFYHEMI+RGFKP+AVTYSIR+DAYCKRGCFVDGLRV +EM+RAKFEPTLETITTLIHG
Sbjct: 241 ELFYHEMIRRGFKPDAVTYSIRMDAYCKRGCFVDGLRVFEEMKRAKFEPTLETITTLIHG 300

Query: 301 AGLTKDKTKARQLFDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHD 360
           AG+ KD  KARQLFDE+ LRNLCPDIGAYNALISSLIRSDDV  AAALMEDMEAKHIGHD
Sbjct: 301 AGVAKDIAKARQLFDEMPLRNLCPDIGAYNALISSLIRSDDVNSAAALMEDMEAKHIGHD 360

Query: 361 SLTYHMMFSGLMRLEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWG 420
           S+TYHMMF GLM+LEDV GFYELY KM+RRNFVPKTRTV+MIMKFFCENRRVDLGL+LWG
Sbjct: 361 SMTYHMMFVGLMKLEDVHGFYELYRKMIRRNFVPKTRTVVMIMKFFCENRRVDLGLDLWG 420

Query: 421 YLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAH 480
           YLVEKGYCPHSHVLD+LVTGLCARGMV +AFECSKQMLERGRQMS+AAFLIM+RSLLQA 
Sbjct: 421 YLVEKGYCPHSHVLDVLVTGLCARGMVHEAFECSKQMLERGRQMSDAAFLIMERSLLQAD 480

Query: 481 ATDNYGELEQLRKKLKTVLPPPNQLSSEISASS 514
           A D  GELEQLRKKLKTVLPPP QL  EI ASS
Sbjct: 481 AKDILGELEQLRKKLKTVLPPPKQLPYEIPASS 510

BLAST of Lsi09G005860 vs. TAIR 10
Match: AT3G61360.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 561.2 bits (1445), Expect = 8.4e-160
Identity = 286/491 (58.25%), Postives = 368/491 (74.95%), Query Frame = 0

Query: 15  ISSHSFLILAAP-FCTHLHLHPSSQPTTEIERIAKIINDHPFPDHPLQPILLHHIPSPLP 74
           ISS S+  L AP   +   L  +S   TEIERI  IIN HPFP+HP+QPIL  HIP    
Sbjct: 5   ISSDSYRRLLAPVLSSSSSLSSTSINRTEIERITIIINGHPFPNHPIQPILAKHIPLSSL 64

Query: 75  SNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFEKTLHILARMRYFDQSW 134
           S  F+++VLGRLFAAHSNGLKALEFFK+ L  S+ SSPT D+FEKTLHILARMRYFDQ+W
Sbjct: 65  SPEFVSEVLGRLFAAHSNGLKALEFFKYSLKSSK-SSPTSDSFEKTLHILARMRYFDQAW 124

Query: 135 ELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVFVGRKFGTEEFNVL 194
            LM E+ + +P LL+ KSMSILL KIAKF S+EET+EAF +ME E+F  +KFG +EFN+L
Sbjct: 125 ALMAEVRKDYPNLLSFKSMSILLCKIAKFGSYEETLEAFVKMEKEIF-RKKFGVDEFNIL 184

Query: 195 LRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAVELFYHEMIKRGFK 254
           LRAFCT+R+MKEARS+F+K++SRF P+ KTMN+LLLGFKE+ DVTA ELFYHEM+KRGFK
Sbjct: 185 LRAFCTEREMKEARSIFEKLHSRFNPDVKTMNILLLGFKEAGDVTATELFYHEMVKRGFK 244

Query: 255 PNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHGAGLTKDKTKARQL 314
           PN+VTY IRID +CK+  F + LR+ ++M+R  F+ T++ +TTLIHG+G+ ++K KARQL
Sbjct: 245 PNSVTYGIRIDGFCKKRNFGEALRLFEDMDRLDFDITVQILTTLIHGSGVARNKIKARQL 304

Query: 315 FDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHDSLTYHMMFSGLMR 374
           FDEI+ R L PD GAYNAL+SSL++  DV  A  +M++ME K I  DS+T+H MF G+M+
Sbjct: 305 FDEISKRGLTPDCGAYNALMSSLMKCGDVSGAIKVMKEMEEKGIEPDSVTFHSMFIGMMK 364

Query: 375 LEDVG--GFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWGYLVEKGYCPHS 434
            ++ G  G  E Y KM  R+ VPKT T++M+MK FC N  V+LGL+LW Y++EKGYCPH 
Sbjct: 365 SKEFGFNGVCEYYQKMKERSLVPKTPTIVMLMKLFCHNGEVNLGLDLWKYMLEKGYCPHG 424

Query: 435 HVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAHATDNYGELEQL 494
           H L+LL T LCAR     AFECS Q +ERGR +SE  + +++ SL   +      EL++ 
Sbjct: 425 HALELLTTALCARRRANDAFECSWQTVERGRCVSEPVYRMLETSLSSNNELKKLEELKEE 484

Query: 495 RKKLKTVLPPP 503
            +KL + LPPP
Sbjct: 485 IQKLHSFLPPP 493

BLAST of Lsi09G005860 vs. TAIR 10
Match: AT1G02420.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 230.3 bits (586), Expect = 3.4e-60
Identity = 138/432 (31.94%), Postives = 231/432 (53.47%), Query Frame = 0

Query: 74  SNTFLNDVLGRLFAAHSNGLKALEFFKFC-----LHHSQASSPTPDAFEKTLHILARMRY 133
           S   ++ VL R+  +H N ++ LEF+++       +HS  S  T       L+IL R R 
Sbjct: 70  SKDLIDRVLKRVRFSHGNPIQTLEFYRYASAIRGFYHSSFSLDT------MLYILGRNRK 129

Query: 134 FDQSWELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVFVGRKFGTE 193
           FDQ WEL+ E  R    L++ ++M ++L ++AK  S  +T+E+F + +    V   F T 
Sbjct: 130 FDQIWELLIETKRKDRSLISPRTMQVVLGRVAKLCSVRQTVESFWKFKR--LVPDFFDTA 189

Query: 194 EFNVLLRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAVELFYHEMI 253
            FN LLR  C ++ M +AR+V+  +  +F P+ +T N+LL G+K S +    E F+ EM 
Sbjct: 190 CFNALLRTLCQEKSMTDARNVYHSLKHQFQPDLQTFNILLSGWKSSEE---AEAFFEEMK 249

Query: 254 KRGFKPNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHGAGLTKDKT 313
            +G KP+ VTY+  ID YCK        +++ +M   +  P + T TT+I G GL     
Sbjct: 250 GKGLKPDVVTYNSLIDVYCKDREIEKAYKLIDKMREEEETPDVITYTTVIGGLGLIGQPD 309

Query: 314 KARQLFDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHDSLTYHMMF 373
           KAR++  E+      PD+ AYNA I +   +  +  A  L+++M  K +  ++ TY++ F
Sbjct: 310 KAREVLKEMKEYGCYPDVAAYNAAIRNFCIARRLGDADKLVDEMVKKGLSPNATTYNLFF 369

Query: 374 SGLMRLEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWGYLVEKGYC 433
             L    D+G  +ELY +M+    +P T++ + ++K F  + +VD+ + LW  +V KG+ 
Sbjct: 370 RVLSLANDLGRSWELYVRMLGNECLPNTQSCMFLIKMFKRHEKVDMAMRLWEDMVVKGFG 429

Query: 434 PHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAHATDNYGEL 493
            +S V D+L+  LC    V +A +C  +M+E+G + S  +F  +K  +  A+  D    L
Sbjct: 430 SYSLVSDVLLDLLCDLAKVEEAEKCLLEMVEKGHRPSNVSFKRIKLLMELANKHDEVNNL 489

Query: 494 EQLRKKLKTVLP 501
            Q      T +P
Sbjct: 490 IQKMAIFSTEIP 490

BLAST of Lsi09G005860 vs. TAIR 10
Match: AT1G71060.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 170.2 bits (430), Expect = 4.2e-42
Identity = 130/501 (25.95%), Postives = 240/501 (47.90%), Query Frame = 0

Query: 6   RLTQIPQIPISSHSFLILAAPFCTHLHLHPSSQPT--------TEIERIAKIINDHPFPD 65
           R++      + S  ++  A+ F  +   H SS  T         + ERI KI+    F D
Sbjct: 21  RISSSSSPSLESIPWIHKASNFTLYGSFHASSVETQVSANDASQDAERICKILT--KFTD 80

Query: 66  HPLQPILLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFE 125
             ++ +L  +  S   S   + +VL +L  A   G+ AL  FK+   + +    T   + 
Sbjct: 81  SKVETLL--NEASVKLSPALIEEVLKKLSNA---GVLALSVFKWA-ENQKGFKHTTSNYN 140

Query: 126 KTLHILARMRYFDQSWELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMEN 185
             +  L +++ F   W L+ ++      LL+ ++ +++  + A+ +  +E I AF +ME 
Sbjct: 141 ALIESLGKIKQFKLIWSLVDDMKAKK--LLSKETFALISRRYARARKVKEAIGAFHKMEE 200

Query: 186 EVFVGRKFGTEEFNVLLRAFCTQRQMKEARSVFQKM-YSRFPPNTKTMNLLLLGFKESTD 245
               G K  + +FN +L      R + +A+ VF KM   RF P+ K+  +LL G+ +  +
Sbjct: 201 ---FGFKMESSDFNRMLDTLSKSRNVGDAQKVFDKMKKKRFEPDIKSYTILLEGWGQELN 260

Query: 246 VTAVELFYHEMIKRGFKPNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITT 305
           +  V+    EM   GF+P+ V Y I I+A+CK   + + +R   EME+   +P+     +
Sbjct: 261 LLRVDEVNREMKDEGFEPDVVAYGIIINAHCKAKKYEEAIRFFNEMEQRNCKPSPHIFCS 320

Query: 306 LIHGAGLTKDKTKARQLFDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKH 365
           LI+G G  K    A + F+         +   YNAL+ +   S  ++ A   +++M  K 
Sbjct: 321 LINGLGSEKKLNDALEFFERSKSSGFPLEAPTYNALVGAYCWSQRMEDAYKTVDEMRLKG 380

Query: 366 IGHDSLTYHMMFSGLMRLEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGL 425
           +G ++ TY ++   L+R++     YE+Y  M   +  P   T  ++++ FC   R+D+ +
Sbjct: 381 VGPNARTYDIILHHLIRMQRSKEAYEVYQTM---SCEPTVSTYEIMVRMFCNKERLDMAI 440

Query: 426 ELWGYLVEKGYCPHSHVLDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSL 485
           ++W  +  KG  P  H+   L+T LC    + +A E   +ML+ G +     F  +K++L
Sbjct: 441 KIWDEMKGKGVLPGMHMFSSLITALCHENKLDEACEYFNEMLDVGIRPPGHMFSRLKQTL 500

Query: 486 L----QAHATDNYGELEQLRK 494
           L    +   TD   ++++LRK
Sbjct: 501 LDEGRKDKVTDLVVKMDRLRK 505

BLAST of Lsi09G005860 vs. TAIR 10
Match: AT1G77360.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 166.8 bits (421), Expect = 4.6e-41
Identity = 107/429 (24.94%), Postives = 213/429 (49.65%), Query Frame = 0

Query: 74  SNTFLNDVLGRLFAAHSNGLKALEFFKFCLHHSQASSPTPDAFEKTLHILARMRYFDQSW 133
           S   + DVL R     + GL    FF++     +    +  A+   +   A++R +   W
Sbjct: 99  SQEVVEDVLNRF---RNAGLLTYRFFQWS-EKQRHYEHSVRAYHMMIESTAKIRQYKLMW 158

Query: 134 ELMREIGRTHPFLLTLKSMSILLSKIAKFQSFEETIEAFQRMENEVFVGRKFGTEEFNVL 193
           +L+  + +    +L +++  I++ K A+ Q  +E I AF  ME             FN L
Sbjct: 159 DLINAMRKKK--MLNVETFCIVMRKYARAQKVDEAIYAFNVMEKYDLPPNLVA---FNGL 218

Query: 194 LRAFCTQRQMKEARSVFQKMYSRFPPNTKTMNLLLLGFKESTDVTAVELFYHEMIKRGFK 253
           L A C  + +++A+ VF+ M  RF P++KT ++LL G+ +  ++      + EMI  G  
Sbjct: 219 LSALCKSKNVRKAQEVFENMRDRFTPDSKTYSILLEGWGKEPNLPKAREVFREMIDAGCH 278

Query: 254 PNAVTYSIRIDAYCKRGCFVDGLRVLKEMERAKFEPTLETITTLIHGAGLTKDKTKARQL 313
           P+ VTYSI +D  CK G   + L +++ M+ +  +PT    + L+H  G      +A   
Sbjct: 279 PDIVTYSIMVDILCKAGRVDEALGIVRSMDPSICKPTTFIYSVLVHTYGTENRLEEAVDT 338

Query: 314 FDEITLRNLCPDIGAYNALISSLIRSDDVKCAAALMEDMEAKHIGHDSLTYHMMFSGLMR 373
           F E+    +  D+  +N+LI +  +++ +K    ++++M++K +  +S + +++   L+ 
Sbjct: 339 FLEMERSGMKADVAVFNSLIGAFCKANRMKNVYRVLKEMKSKGVTPNSKSCNIILRHLIE 398

Query: 374 LEDVGGFYELYSKMVRRNFVPKTRTVIMIMKFFCENRRVDLGLELWGYLVEKGYCPHSHV 433
             +    ++++ KM+ +   P   T  M++K FCE + ++   ++W Y+ +KG  P  H 
Sbjct: 399 RGEKDEAFDVFRKMI-KVCEPDADTYTMVIKMFCEKKEMETADKVWKYMRKKGVFPSMHT 458

Query: 434 LDLLVTGLCARGMVLQAFECSKQMLERGRQMSEAAFLIMKRSLLQAHATDNYGELEQLRK 493
             +L+ GLC      +A    ++M+E G + S   F  +++ L++    D    L+ L +
Sbjct: 459 FSVLINGLCEERTTQKACVLLEEMIEMGIRPSGVTFGRLRQLLIKEERED---VLKFLNE 514

Query: 494 KLKTVLPPP 503
           K+  ++  P
Sbjct: 519 KMNVLVNEP 514

BLAST of Lsi09G005860 vs. TAIR 10
Match: AT1G74900.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 148.3 bits (373), Expect = 1.7e-35
Identity = 102/406 (25.12%), Postives = 187/406 (46.06%), Query Frame = 0

Query: 39  PTTEIERIAKIINDHPFPDHPLQPILLHHIPSPLPSNTFLNDVLGRLFAAHSNGLKALEF 98
           P  +   IAK+I   P   H     LL    +P   N  +N VL RL+   ++G KAL+F
Sbjct: 21  PPADSAAIAKLILSSPNTTHQDDQFLLSTKTTPWTPN-LVNSVLKRLW---NHGPKALQF 80

Query: 99  FKFCLHHSQASSPTPDAFEKTLHILARMRYFDQSWELMREIGRTHPFLLTLKSMSILLSK 158
           F F  +H +       +F+  + I AR+      W L+  + R+     + K+ +I+  +
Sbjct: 81  FHFLDNHHREYVHDASSFDLAIDIAARLHLHPTVWSLIHRM-RSLRIGPSPKTFAIVAER 140

Query: 159 IAKFQSFEETIEAFQRMENEVFVGRKFGTEEFNVLLRAFCTQRQMKEARSVFQKMYSRFP 218
            A     ++ ++ F  M      G       FN +L   C  +++++A  +F+ +  RF 
Sbjct: 141 YASAGKPDKAVKLFLNMHEH---GCFQDLASFNTILDVLCKSKRVEKAYELFRALRGRFS 200

Query: 219 PNTKTMNLLLLGFKESTDVTAVELFYHEMIKRGFKPNAVTYSIRIDAYCKRGCFVDGLRV 278
            +T T N++L G+              EM++RG  PN  TY+  +  + + G        
Sbjct: 201 VDTVTYNVILNGWCLIKRTPKALEVLKEMVERGINPNLTTYNTMLKGFFRAGQIRHAWEF 260

Query: 279 LKEMERAKFEPTLETITTLIHGAGLTKDKTKARQLFDEITLRNLCPDIGAYNALISSLIR 338
             EM++   E  + T TT++HG G+  +  +AR +FDE+    + P +  YNA+I  L +
Sbjct: 261 FLEMKKRDCEIDVVTYTTVVHGFGVAGEIKRARNVFDEMIREGVLPSVATYNAMIQVLCK 320

Query: 339 SDDVKCAAALMEDMEAKHIGHDSLTYHMMFSGLMRLEDVGGFYELYSKMVRRNFVPKTRT 398
            D+V+ A  + E+M  +    +  TY+++  GL    +     EL  +M      P  +T
Sbjct: 321 KDNVENAVVMFEEMVRRGYEPNVTTYNVLIRGLFHAGEFSRGEELMQRMENEGCEPNFQT 380

Query: 399 VIMIMKFFCENRRVDLGLELWGYLVEKGYCPHSHVLDLLVTGLCAR 445
             M+++++ E   V+  L L+  +      P+    ++L++G+  R
Sbjct: 381 YNMMIRYYSECSEVEKALGLFEKMGSGDCLPNLDTYNILISGMFVR 418

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M2C81.2e-15858.25Pentatricopeptide repeat-containing protein At3g61360 OS=Arabidopsis thaliana OX... [more]
Q9FZ194.8e-5931.94Putative pentatricopeptide repeat-containing protein At1g02420 OS=Arabidopsis th... [more]
Q9C9A25.9e-4125.95Pentatricopeptide repeat-containing protein At1g71060, mitochondrial OS=Arabidop... [more]
Q9FVX26.5e-4024.94Pentatricopeptide repeat-containing protein At1g77360, mitochondrial OS=Arabidop... [more]
Q9S7R41.1e-3425.12Pentatricopeptide repeat-containing protein At1g74900, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A5A7UJI25.6e-26089.06Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A5D3BMK09.5e-26089.06Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S4DS469.5e-26089.06pentatricopeptide repeat-containing protein At3g61360 OS=Cucumis melo OX=3656 GN... [more]
A0A6J1EM608.3e-25687.91pentatricopeptide repeat-containing protein At3g61360 OS=Cucurbita moschata OX=3... [more]
A0A0A0KRS81.7e-25388.62Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G173440 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038876095.12.0e-27291.62pentatricopeptide repeat-containing protein At3g61360 [Benincasa hispida] >XP_03... [more]
KAA0055168.11.1e-25989.06pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa][more]
TYK00295.12.0e-25989.06pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa][more]
XP_016898809.12.0e-25989.06PREDICTED: pentatricopeptide repeat-containing protein At3g61360 [Cucumis melo][more]
XP_022929103.11.7e-25587.91pentatricopeptide repeat-containing protein At3g61360 [Cucurbita moschata] >XP_0... [more]
Match NameE-valueIdentityDescription
AT3G61360.18.4e-16058.25Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G02420.13.4e-6031.94Pentatricopeptide repeat (PPR) superfamily protein [more]
AT1G71060.14.2e-4225.95Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G77360.14.6e-4124.94Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G74900.11.7e-3525.12Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 190..216
e-value: 1.3E-4
score: 19.9
coord: 257..290
e-value: 1.2E-8
score: 32.6
coord: 363..394
e-value: 6.1E-4
score: 17.8
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 190..214
e-value: 0.0028
score: 17.8
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 324..372
e-value: 7.7E-8
score: 32.4
coord: 219..268
e-value: 2.9E-10
score: 40.2
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 360..394
score: 9.45966
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 220..254
score: 9.054091
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 186..216
score: 8.527949
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 255..289
score: 11.673842
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 84..282
e-value: 3.0E-26
score: 94.6
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 304..511
e-value: 3.6E-29
score: 104.1
NoneNo IPR availablePANTHERPTHR45613PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 29..500
NoneNo IPR availablePANTHERPTHR45613:SF143OS06G0694600 PROTEINcoord: 29..500

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G005860.1Lsi09G005860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005515 protein binding