Homology
BLAST of Lsi09G003440 vs. ExPASy Swiss-Prot
Match:
A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)
HSP 1 Score: 901.7 bits (2329), Expect = 5.4e-261
Identity = 461/749 (61.55%), Postives = 568/749 (75.83%), Query Frame = 0
Query: 11 FLLNFHGNVA----MKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYTSFLPKT 70
FLL FH + A + T ++++ T + YI+HV PE + ++ DLESWY SFLP T
Sbjct: 11 FLLTFHVHNAQGSELPTTTTESTETSSSKIYIIHVTGPEGKMLTESEDLESWYHSFLPPT 70
Query: 71 IETSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYLG 130
+ +S EQ R++YSY++V+ GF+A LT+E++ ++E+K+GFISA P+ +L+ TTHTP++LG
Sbjct: 71 LMSSEEQPRVIYSYKNVLRGFAASLTQEELSAVEKKNGFISAHPQRVLHRQTTHTPKFLG 130
Query: 131 LNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKL 190
L Q G+WK+SNFGKGVIIGVLD+GI P HPSF+D G+P PP KWKGRC+ + CNNKL
Sbjct: 131 LQQDTGVWKESNFGKGVIIGVLDSGITPGHPSFSDVGIPPPPPKWKGRCDLNVTACNNKL 190
Query: 191 IGARTFNLANTVLMG---ETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLA 250
IGAR FNLA + G E PIDE+GHGTHTASTAAG FV AE LGNA+G A G+AP A
Sbjct: 191 IGARAFNLAAEAMNGKKAEAPIDEDGHGTHTASTAAGAFVNYAEVLGNAKGTAAGMAPHA 250
Query: 251 HVAMYKVC-SKGCQSSDILAGLDAAIDDGVDVLSISIGSGSGYSLVDDDVAIGAFAAIKK 310
H+A+YKVC + C SDILA LDAA++DGVDV+SIS+G +D AIGAFAA++K
Sbjct: 251 HLAIYKVCFGEDCPESDILAALDAAVEDGVDVISISLGLSEPPPFFNDSTAIGAFAAMQK 310
Query: 311 GIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIAALAKLGNDEVHVGESLFQPRDF 370
GIFVSC+AGNSGP S++ N APWILTVGASTIDR I A AKLGN + GES+FQP F
Sbjct: 311 GIFVSCAAGNSGPFNSSIVNAAPWILTVGASTIDRRIVATAKLGNGQEFDGESVFQPSSF 370
Query: 371 TSTFLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAA 430
T T LPL YAG +G + S FC GSL + +GKVV+CE GG I R+ KG VK AGGAA
Sbjct: 371 TPTLLPLAYAGKNGKEESAFCANGSLDDSAFRGKVVLCERGGGIARIAKGEEVKRAGGAA 430
Query: 431 MILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTD 490
MIL+N + N F+ SAD H LPAT VSY AG++IKAYI+S+ PTA+I FKGT+IG S
Sbjct: 431 MILMNDETNAFSLSADVHALPATHVSYAAGIEIKAYINSTATPTATILFKGTVIGNS--- 490
Query: 491 FSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPIDSNKNTKSTFNVMSGTSMSC 550
+PA+ASFSSRGP L S GILKPDI GPGVNILAAWPFP+ ++ ++K TFN+ SGTSMSC
Sbjct: 491 LAPAVASFSSRGPNLPSPGILKPDIIGPGVNILAAWPFPLSNSTDSKLTFNIESGTSMSC 550
Query: 551 PHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGSGHVNP 610
PHLSGIAAL+K+ HP+WSPAAIKSAIMTSAD K IVD+ L+P + FATGSGHVNP
Sbjct: 551 PHLSGIAALLKSSHPHWSPAAIKSAIMTSADTINLGNKLIVDETLQPTDLFATGSGHVNP 610
Query: 611 SKATDPGLVYDIQADDYVRYLCGL-YTDVQVSMIVRRQITCSTVSKIREGDLNYPSFSVT 670
S+A DPGLVYDIQ DDY+ YLCGL Y++ +V +I R+I CS + I EG+LNYPSFSV
Sbjct: 611 SRANDPGLVYDIQPDDYIPYLCGLGYSETEVGIIAHRKIKCS--ASIPEGELNYPSFSVE 670
Query: 671 LGASQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDAL 730
LG+S+TF+RTVTNVG+A+S Y IV AP GV V+V P L FS +N+K TYSVTFS L
Sbjct: 671 LGSSKTFTRTVTNVGEAHSSYDLIVAAPQGVDVKVQPYKLNFSEVNQKETYSVTFSRTGL 730
Query: 731 VKVTSEFSEGYLKWVSDKHLVRSPISVKF 751
T E+++G+LKWVS KH VRSPISVKF
Sbjct: 731 GNKTQEYAQGFLKWVSTKHTVRSPISVKF 754
BLAST of Lsi09G003440 vs. ExPASy Swiss-Prot
Match:
A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)
HSP 1 Score: 827.4 bits (2136), Expect = 1.3e-238
Identity = 437/757 (57.73%), Postives = 550/757 (72.66%), Query Frame = 0
Query: 1 MVVLPLFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYT 60
M + +F I FLL+FH A ++ LQTYIVHV +P+ + ++ DLES+Y
Sbjct: 1 MTCICIFSIAFLLSFHLTTAQRST--------GLQTYIVHVDKPDAQVLANSADLESYYN 60
Query: 61 SFLPKTIETSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTH 120
SFLP T+ S SR+++SY HV +GF+A+L+ E+VK ME+K GF+SA+ E +L LHTTH
Sbjct: 61 SFLPATVSGSEVPSRIIHSYHHVATGFAAKLSGEEVKEMEKKAGFVSAKLEKVLTLHTTH 120
Query: 121 TPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEF-GA 180
TP +LGL Q G W++SN+GKGVIIG+LDTGI P HPSF+D MP PPAKWKG+CEF G
Sbjct: 121 TPNFLGLYQNMGFWQESNYGKGVIIGLLDTGITPGHPSFSDVNMPSPPAKWKGKCEFTGN 180
Query: 181 SICNNKLIGARTFNLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGI 240
+ CN K+IGAR F + G P DE GHGTHTASTAAG FV A GNA G AVG+
Sbjct: 181 ATCNKKIIGARNFISGS----GVPPTDEEGHGTHTASTAAGNFVNDANVFGNANGTAVGM 240
Query: 241 APLAHVAMYKVCSK-GCQSSDILAGLDAAIDDGVDVLSISIGSGSGYS--LVDDDVAIGA 300
APLAH+AMYKVCS+ GC +DILA LDAAIDDGVDVLS+S+G GYS D++AIGA
Sbjct: 241 APLAHIAMYKVCSEDGCSDADILAALDAAIDDGVDVLSLSLG---GYSDPFYYDNIAIGA 300
Query: 301 FAAIKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIAALAKLGNDEVHVGESL 360
FAAI+KGIFVS SAGN GP STL+NEAPWILTVGAST DR I A A LGN + + GES
Sbjct: 301 FAAIRKGIFVSASAGNDGPLNSTLSNEAPWILTVGASTHDRKIVATAVLGNGQQYDGESA 360
Query: 361 FQPRDFTSTFLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVK 420
FQP DF T LPLVY G S + + FC GSL DVKGKVVVC+ GG++ R++K VK
Sbjct: 361 FQPADFPHTLLPLVYPGTS-DEEAAFCSSGSLDKFDVKGKVVVCDRGGDVARLEKSQTVK 420
Query: 421 NAGGAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTII 480
+AGGAAMIL N + +G T ADAHVLPAT V Y AG IK+YI+S+ PTA I FKGTII
Sbjct: 421 DAGGAAMILANLEIDGEGTFADAHVLPATHVGYAAGEMIKSYINSTSTPTAGILFKGTII 480
Query: 481 GGSNTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPIDSNKNTKSTFNVMS 540
G + SP+++SFSSRGP L+S GI+KPDI GPGVNILAAWP +++ T TFN++S
Sbjct: 481 GFKS---SPSVSSFSSRGPNLASPGIVKPDIIGPGVNILAAWPVSVENKTGTDLTFNIIS 540
Query: 541 GTSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATG 600
GTSMSCPHLSGI AL+K+ HP+WSPAAIKSAIMTSAD +G+PI+D+ PA+ FATG
Sbjct: 541 GTSMSCPHLSGIVALLKSAHPDWSPAAIKSAIMTSADQSNLEGQPILDERNLPADIFATG 600
Query: 601 SGHVNPSKATDPGLVYDIQADDYVRYLCGL-YTDVQVSMIVRRQITCSTVSKIREGDLNY 660
+GHVNPSKA+DPGL+YDIQ +DY++YLCGL Y + + +IV+ + C S I E +LNY
Sbjct: 601 AGHVNPSKASDPGLIYDIQLEDYIQYLCGLGYREKDIGLIVQETVKCE--SSISEAELNY 660
Query: 661 PSFSVTLG-ASQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSV 720
PSFS+ LG +Q ++RTVTNVGDA+S Y+ + GV + V P+ L F+ + ++ TY+V
Sbjct: 661 PSFSIILGPKTQNYTRTVTNVGDASSTYTVNIAKTQGVDIVVEPATLVFTKMYQQATYTV 720
Query: 721 TFSVDALVKVTSEFSEGYLKWVSDKHLVRSPISVKFK 752
+F+ T F +G + W S++++VRSPISVK +
Sbjct: 721 SFTQSG--DFTDRFVQGAISWSSNEYVVRSPISVKLE 734
BLAST of Lsi09G003440 vs. ExPASy Swiss-Prot
Match:
A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)
HSP 1 Score: 808.9 bits (2088), Expect = 4.8e-233
Identity = 424/729 (58.16%), Postives = 542/729 (74.35%), Query Frame = 0
Query: 27 DTSNTIKLQTYIVHVQQPELESSDDTFDLESWYTSFLPKTIETSNEQSRLLYSYRHVMSG 86
D SN L TYIVHV++P++ SD DL ++Y S LP++ +T+N+ R++++YR+V++G
Sbjct: 38 DQSN---LSTYIVHVRKPQVIQSD---DLHTFYYSLLPESTKTTNQ--RIVFTYRNVVNG 97
Query: 87 FSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGKGVIIG 146
F+ +LT E+ K++++ + +SARPE IL+LHTTHTP +LGL Q GLWK SN GKGVIIG
Sbjct: 98 FAVKLTPEEAKALQQNEEVVSARPEKILSLHTTHTPSFLGLQQGLGLWKGSNSGKGVIIG 157
Query: 147 VLDTGINPDHPSFNDDGMPQPPAKWKGRCEF-GASICNNKLIGARTFNLANTVLMGETPI 206
+LDTGI+P HPSF+D+GMP PPAKW G CEF G CNNK+IGAR F + + P
Sbjct: 158 ILDTGISPFHPSFSDEGMPSPPAKWNGICEFTGKRTCNNKIIGARNFVKTKNLTL---PF 217
Query: 207 DENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLAHVAMYKVCS-KGCQSSDILAGL 266
D+ GHGTHTASTAAG V+GA GNA G AVG+AP AH+AMYKVC GC S ILAG+
Sbjct: 218 DDVGHGTHTASTAAGRPVQGANVYGNANGTAVGMAPDAHIAMYKVCGLVGCSESAILAGM 277
Query: 267 DAAIDDGVDVLSISIGSGSGYSLVDDDVAIGAFAAIKKGIFVSCSAGNSGPSKSTLANEA 326
D A+DDGVDVLS+S+G SG +D +A+GAF AI+KGIFVSCSA NSGP+ S+L+NEA
Sbjct: 278 DTAVDDGVDVLSLSLGGPSG-PFFEDPIALGAFGAIQKGIFVSCSAANSGPAYSSLSNEA 337
Query: 327 PWILTVGASTIDRTIAALAKLGNDEVHVGESLFQPRDFTSTFLPLVYAGNSGTKR-SEFC 386
PWILTVGAS+IDRTI A AKLGN + +VG+S+FQP+DF + LPLVYAG +G S FC
Sbjct: 338 PWILTVGASSIDRTIMATAKLGNGKEYVGQSVFQPKDFAPSLLPLVYAGANGNNNFSVFC 397
Query: 387 VQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAAMILINQKPNGFTTSADAHVLP 446
SL DV+GKVV+CE GG + RV KG VK+AGGAAMIL+N F AD HVLP
Sbjct: 398 APESLNRSDVEGKVVLCEDGGFVPRVFKGKAVKDAGGAAMILMNSVLEDFNPIADVHVLP 457
Query: 447 ATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTDFSPAMASFSSRGPCLSSHGIL 506
A +SYEAGL +K YI+S+ PTA+I F+GT+IG +P + SFSSRGP +S GIL
Sbjct: 458 AVHISYEAGLALKEYINSTSTPTATILFEGTVIGNL---LAPQVTSFSSRGPSKASPGIL 517
Query: 507 KPDITGPGVNILAAWPFPIDSNKNTKSTFNVMSGTSMSCPHLSGIAALIKNVHPNWSPAA 566
KPDI GPG+NILAAWP +D+ +T FN++SGTSMSCPHLSGIAAL+KN HP+WSPAA
Sbjct: 518 KPDIIGPGLNILAAWPVSLDN--STTPPFNIISGTSMSCPHLSGIAALLKNSHPDWSPAA 577
Query: 567 IKSAIMTSADVKTPQGKPIVDQDLKPANFFATGSGHVNPSKATDPGLVYDIQADDYVRYL 626
IKSAIMT+A G PI+DQ L PA+ FATG+GHVNP KA DPGLVYDI+ +DY+ YL
Sbjct: 578 IKSAIMTTASQVNLGGTPILDQRLVPADVFATGAGHVNPVKANDPGLVYDIEPNDYIPYL 637
Query: 627 CGL-YTDVQVSMIVRRQITCSTVSKIREGDLNYPSFSVTLG-ASQTFSRTVTNVGDANSV 686
CGL YTD +V +I+++++ CS V+ I E +LNYPSFS+ LG +Q ++RTV NVG ANS
Sbjct: 638 CGLNYTDREVGVILQQRVRCSEVNHIAEAELNYPSFSILLGNTTQLYTRTVANVGPANST 697
Query: 687 YSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDALVKVTSEFSEGYLKWVSDKHL 746
Y+A + PVGV + ++P+ L F+ + +K+TYSV+F + + F++G LKWVS K+
Sbjct: 698 YTAEIGVPVGVGMSLSPAQLTFTEVGQKLTYSVSFIPFSEDRDNHTFAQGSLKWVSGKYS 749
Query: 747 VRSPISVKF 751
VRSPIS F
Sbjct: 758 VRSPISFIF 749
BLAST of Lsi09G003440 vs. ExPASy Swiss-Prot
Match:
A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)
HSP 1 Score: 796.6 bits (2056), Expect = 2.5e-229
Identity = 425/727 (58.46%), Postives = 534/727 (73.45%), Query Frame = 0
Query: 34 LQTYIVHVQQPELESS-DDTFDLESWYTSFLPKTIETSNEQSRLLYSYRHVMSGFSARLT 93
L TYIVHV++ E+E T +L +W+ SFLP ETSN + R+++SYR+V SGF+ RLT
Sbjct: 38 LTTYIVHVKKLEIEGPLQSTEELHTWHHSFLP---ETSN-KDRMVFSYRNVASGFAVRLT 97
Query: 94 EEQVKSMEEKDGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGI 153
E+ +++EK+ +S RPE L+LHTTHTP +LGL Q GLW DSN GKGVIIGV+DTGI
Sbjct: 98 PEEANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDTGI 157
Query: 154 NPDHPSFNDDGMPQPPAKWKGRCEF-GASICNNKLIGARTFNLANTVLMGETPIDENGHG 213
P H SFND+GMP PPAKWKG CEF G S+CNNKLIGAR NL + + E P ++ HG
Sbjct: 158 YPFHLSFNDEGMPPPPAKWKGHCEFTGGSVCNNKLIGAR--NLVKSAIQ-EPPYEDFFHG 217
Query: 214 THTASTAAGTFVEGAEALGNARGKAVGIAPLAHVAMYKVCSK----GCQSSDILAGLDAA 273
THTA+ AAG FVEGA GNARG A G+AP AH+A+YKVCS C S ILA +D A
Sbjct: 218 THTAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAILAAMDIA 277
Query: 274 IDDGVDVLSISIGSGSGYSLVDDDVAIGAFAAIKKGIFVSCSAGNSGPSKSTLANEAPWI 333
I+DGVDVLS+S+G GS +D +AIGAFAA +KGIFVSCSA NSGP S+L+NEAPWI
Sbjct: 278 IEDGVDVLSLSLGLGS-LPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLSNEAPWI 337
Query: 334 LTVGASTIDRTIAALAKLGNDEVHVGESLFQPRDFTSTFLPLVYAGNSGTKRSEFCVQGS 393
LTVGASTIDR I+A AKLGN + GE+LFQP+DF+S LPLVYA S C GS
Sbjct: 338 LTVGASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQLLPLVYAAAEKNNSSALCAPGS 397
Query: 394 LANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAAMILINQKPNGFTTSADAHVLPATRV 453
L N++VKGKVVVC+ GG I + KG V +AGG+AMIL N + GFTT A+AHVLPA V
Sbjct: 398 LRNINVKGKVVVCDLGGGIPFIAKGQEVLDAGGSAMILANIENFGFTTLANAHVLPAVHV 457
Query: 454 SYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTDFSPAMASFSSRGPCLSSHGILKPDI 513
SY A L IKAYI+S+ PTA++ F+GTIIG S +P++A+FSSRGP S GILKPDI
Sbjct: 458 SYAASLAIKAYINSTYTPTATVLFQGTIIGDS---LAPSVAAFSSRGPSQQSPGILKPDI 517
Query: 514 TGPGVNILAAWPFPIDSNKNTKSTFNVMSGTSMSCPHLSGIAALIKNVHPNWSPAAIKSA 573
GPGVNILAAW +D N F+++SGTSMSCPHLSGIAAL+K+ HP+WSPAAIKSA
Sbjct: 518 IGPGVNILAAWAVSVD---NKIPAFDIISGTSMSCPHLSGIAALLKSAHPDWSPAAIKSA 577
Query: 574 IMTSADVKTPQGKPIVDQDLKPANFFATGSGHVNPSKATDPGLVYDIQADDYVRYLCGL- 633
IMT+A+ +G PI+DQ L+PA+ FATG+GHVNP +A DPGLVYDIQ +DYV YLCGL
Sbjct: 578 IMTTANTLNLRGLPILDQRLQPADIFATGAGHVNPVRANDPGLVYDIQPEDYVPYLCGLG 637
Query: 634 YTDVQVSMIVRRQITCSTVSKIREGDLNYPSFSVTLGA-SQTFSRTVTNVGDANSVYSAI 693
Y+D +V++IV+R + C V I + +LNYPSFS+ LG+ SQ ++RT+TNVG ANS Y+
Sbjct: 638 YSDREVTIIVQRSVRCFNVKSIAQAELNYPSFSILLGSDSQFYTRTLTNVGPANSTYTVK 697
Query: 694 VEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDALV-KVTSEFSEGYLKWVSDKHLVRS 752
++ P+ + + V+PS + F+ +N+K+ Y V F + F++G + WVSDKH+VR+
Sbjct: 698 IDVPLAMGISVSPSQITFTQVNQKVAYFVDFIPQIKENRGNHTFAQGAITWVSDKHVVRT 750
BLAST of Lsi09G003440 vs. ExPASy Swiss-Prot
Match:
G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)
HSP 1 Score: 790.8 bits (2041), Expect = 1.3e-227
Identity = 427/756 (56.48%), Postives = 533/756 (70.50%), Query Frame = 0
Query: 6 LFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYTSFLPK 65
L + F+L +A + N+ + I L TYIVHV++ E +S + DL SWY SFLP+
Sbjct: 9 LVSLIFILCSISMLAAEENLE--HDQINLMTYIVHVKKSENVASHQSEDLHSWYHSFLPQ 68
Query: 66 TIETSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYL 125
T + R+++SYR V SGF+ +LT E+ KS++EK +SARPE L LHTTHTP +L
Sbjct: 69 TFP---HKERMVFSYRKVASGFAVKLTPEEAKSLQEKGEIVSARPERTLELHTTHTPTFL 128
Query: 126 GLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEF-GASICNN 185
GL Q GLW D N GKGVIIG++DTGI P HPSFND+GMP PPAKWKG CEF G +CNN
Sbjct: 129 GLKQGQGLWSDDNLGKGVIIGIIDTGIFPLHPSFNDEGMPPPPAKWKGHCEFTGGQVCNN 188
Query: 186 KLIGARTFNLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLAH 245
KLIGAR NL + + E P + HGTHTA+ AAG F+E A GNA+G A G+AP AH
Sbjct: 189 KLIGAR--NLVKSAIQ-EPPFENFFHGTHTAAEAAGRFIEDASVFGNAKGVAAGMAPNAH 248
Query: 246 VAMYKVCSK--GCQSSDILAGLDAAIDDGVDVLSISIGSGSGYSLVDDDVAIGAFAAIKK 305
+A+YKVC+ GC S ILA +D AI+DGVDVLS+S+G GS +D +AIGAFAA +
Sbjct: 249 LAIYKVCNDKIGCTESAILAAMDIAIEDGVDVLSLSLGLGS-LPFFEDPIAIGAFAATQN 308
Query: 306 GIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIAALAKLGNDEVHVGESLFQPRDF 365
G+FVSCSA NSGP STL+NEAPWILTVGASTIDR I A AKLGN E + GE+LFQP+DF
Sbjct: 309 GVFVSCSAANSGPGYSTLSNEAPWILTVGASTIDRKIVASAKLGNGEEYEGETLFQPKDF 368
Query: 366 TSTFLPLVYAGNSG----TKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNA 425
+ LPLVY G+ G T+ C+ GSL N+D+ GKVV+C+ GN+ + KG V N+
Sbjct: 369 SQQLLPLVYPGSFGYGNQTQNQSLCLPGSLKNIDLSGKVVLCDV-GNVSSIVKGQEVLNS 428
Query: 426 GGAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGG 485
GG AMIL N + GF+T A AHVLPA VSY AGL IK+YI S+ NPTA++ FKGTIIG
Sbjct: 429 GGIAMILANSEALGFSTFAIAHVLPAVEVSYAAGLTIKSYIKSTYNPTATLIFKGTIIGD 488
Query: 486 SNTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPIDSNKNTKSTFNVMSGT 545
S +P++ FSSRGP S GILKPDI GPGVNILAAW +D N F+++SGT
Sbjct: 489 S---LAPSVVYFSSRGPSQESPGILKPDIIGPGVNILAAWAVSVD---NKIPAFDIVSGT 548
Query: 546 SMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGSG 605
SMSCPHLSGIAALIK+ HP+WSPAAIKSAIMT+A+ G PI+DQ L PA+ FATG+G
Sbjct: 549 SMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANTLNLGGIPILDQRLFPADIFATGAG 608
Query: 606 HVNPSKATDPGLVYDIQADDYVRYLCGL-YTDVQVSMIVRRQITCSTVSKIREGDLNYPS 665
HVNP KA DPGLVYDI+ +DYV YLCGL Y+D ++ +IV+ ++ CS V I E LNYPS
Sbjct: 609 HVNPVKANDPGLVYDIEPEDYVPYLCGLGYSDKEIEVIVQWKVKCSNVKSIPEAQLNYPS 668
Query: 666 FSVTLGA-SQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTF 725
FS+ LG+ SQ ++RT+TNVG ANS Y +E P+ + + V PS + F+ +NEK+++SV F
Sbjct: 669 FSILLGSDSQYYTRTLTNVGFANSTYKVELEVPLALGMSVNPSEITFTEVNEKVSFSVEF 728
Query: 726 SVDALV-KVTSEFSEGYLKWVSDKHLVRSPISVKFK 752
+ F +G L WVSD+H VR PISV FK
Sbjct: 729 IPQIKENRRNHTFGQGSLTWVSDRHAVRIPISVIFK 748
BLAST of Lsi09G003440 vs. ExPASy TrEMBL
Match:
A0A1S3AT16 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482683 PE=3 SV=1)
HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 602/754 (79.84%), Postives = 663/754 (87.93%), Query Frame = 0
Query: 1 MVVLPLFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYT 60
MV LP CIFF LNFHG VAMKT +S TSNT LQTYIVHV+QP LE DT DL++WYT
Sbjct: 1 MVFLPFICIFFFLNFHGYVAMKTELSSTSNT-NLQTYIVHVKQPVLEILGDTIDLQNWYT 60
Query: 61 SFLPKTIE-TSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTT 120
SFLP+TIE +S+EQ RLLYSYRHVMSGFSARLT+EQVK+MEEKDGFISA PET LNLHTT
Sbjct: 61 SFLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTT 120
Query: 121 HTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGA 180
HTPEYLGLN+ FGLWK+SNFGKGVIIGVLDTGI+P+HPSFND+GM PPAKWKGRCEFGA
Sbjct: 121 HTPEYLGLNKHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGA 180
Query: 181 SICNNKLIGARTFNLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGI 240
SICNNKLIGARTFNLAN ++G++P DENGHGTHTASTAAGTFV+GAEALGNARGKAVG+
Sbjct: 181 SICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGM 240
Query: 241 APLAHVAMYKVCS-KGCQSSDILAGLDAAIDDGVDVLSISIGSGSGYSLVDDDVAIGAFA 300
APLAH+AMYKVCS GC SSDILA LDAAIDDGVDVLS+S+G+ S D +A+GAFA
Sbjct: 241 APLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPS-TPFFRDTIAMGAFA 300
Query: 301 AIKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIAALAKLGNDEVHVGESLFQ 360
AIKKGIFVSCSAGNSGPSK+TLANEAPWILTVGASTIDR I ALAKLG+ EV+ GESLFQ
Sbjct: 301 AIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQ 360
Query: 361 PRDFTSTFLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNA 420
PR+F S FLPLVYAG SG + SE+C+QGSL L+V GKVVVCE GG I RV KGLVVKN
Sbjct: 361 PRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNG 420
Query: 421 GGAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGG 480
GGAAMILINQKP GF+T A+AHVLP T +SYE GLKIKAYI+SS NP ASISFKGT++G
Sbjct: 421 GGAAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGN 480
Query: 481 SNTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPI--DSNKNTKSTFNVMS 540
T FSPAMASFSSRGPC +S GILKPDITGPGVNILAAWPFP+ ++N NTKSTFNV+S
Sbjct: 481 RATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVIS 540
Query: 541 GTSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATG 600
GTSMSCPHLSGIAALIK+ HPNWSPAAIKSAIMTSADV+ PQGKPI+DQDLKPANFFA G
Sbjct: 541 GTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMG 600
Query: 601 SGHVNPSKATDPGLVYDIQADDYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYP 660
SGHVNPSKA +PGLVYDIQ DDY+ YLC LYTD QVS+IVR+Q+TCSTV +IREGDLNYP
Sbjct: 601 SGHVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYP 660
Query: 661 SFSVTLGA-SQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVT 720
SF+V+LGA SQTF+RTVTNVGDANSVY IVEAP GVSV+VTPSNLKFS LNEK+TYSVT
Sbjct: 661 SFAVSLGAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVT 720
Query: 721 FSVDALVKVTSEFSEGYLKWVSDKHLVRSPISVK 750
FS V+ TSEFSEGYL WVS K +VRSPISVK
Sbjct: 721 FSRIDFVRTTSEFSEGYLIWVSKKLMVRSPISVK 752
BLAST of Lsi09G003440 vs. ExPASy TrEMBL
Match:
A0A0A0KMQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157280 PE=3 SV=1)
HSP 1 Score: 1154.0 bits (2984), Expect = 0.0e+00
Identity = 587/734 (79.97%), Postives = 651/734 (88.69%), Query Frame = 0
Query: 21 MKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYTSFLPKTIE-TSNEQSRLLYS 80
MKT + SNT LQTYIVHV+QPE+E DT DL++WYTSFLP+TIE +SNEQSRLLYS
Sbjct: 1 MKTELWSASNT-NLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYS 60
Query: 81 YRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNF 140
YRHV+SGFSARLT+EQVK+MEEKDGFISA PET LNLHTTHTPEYLGLNQ FGLWK+SNF
Sbjct: 61 YRHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNF 120
Query: 141 GKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNLANTVL 200
GKGVIIGVLDTGI+P+HPSFND+GMP PPAKWKGRCEFGASICNNKLIGARTFNLAN V
Sbjct: 121 GKGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVS 180
Query: 201 MGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLAHVAMYKVCS-KGCQSS 260
+G++P DENGHGTHTASTAAGTFV+GAEALGNARGKAVG+APLAH+A+YKVCS KGC SS
Sbjct: 181 IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSS 240
Query: 261 DILAGLDAAIDDGVDVLSISIGSGSGYSLVDDDVAIGAFAAIKKGIFVSCSAGNSGPSKS 320
DILA LDAAIDDGVDVLS+S+G+ S D +A+GAFAAIKKGIFVSCSAGNSGPSK+
Sbjct: 241 DILAALDAAIDDGVDVLSLSLGAPS-TPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKN 300
Query: 321 TLANEAPWILTVGASTIDRTIAALAKLGNDEVHVGESLFQPRDFTSTFLPLVYAGNSGTK 380
TLANEAPWILTVGASTIDR I ALAKL + +V GESLFQPRDF+S FLPLVYAG SG +
Sbjct: 301 TLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIE 360
Query: 381 RSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAAMILINQKPNGFTTSAD 440
SE+CV+GSL L+V GK+VVCE GG IGR+ KGLVVKN GGAAMIL+NQKP+GF+T A+
Sbjct: 361 GSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAE 420
Query: 441 AHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTDFSPAMASFSSRGPCLS 500
AHVLP T +SYE GLKIK YI+SS NP ASISF+GT++G T FSPAMASFSSRGPC +
Sbjct: 421 AHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQA 480
Query: 501 SHGILKPDITGPGVNILAAWPFPI--DSNKNTKSTFNVMSGTSMSCPHLSGIAALIKNVH 560
S GILKPDITGPGVNILAAWPFP+ ++N NTKSTFNV+SGTSMSCPHLSGIAALIK+ H
Sbjct: 481 SPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNH 540
Query: 561 PNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGSGHVNPSKATDPGLVYDIQA 620
PNWSPAAIKSAIMTSADV+ PQGKPIVDQDLKPANFFA GSGHVNPSKA +PGLVYDIQ
Sbjct: 541 PNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQP 600
Query: 621 DDYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYPSFSVTLGA-SQTFSRTVTNV 680
DDYV YLC LYTD QVS+IVRRQ+TCSTVS+IREGDLNYPSF+V+LGA SQ F+RTVTNV
Sbjct: 601 DDYVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNV 660
Query: 681 GDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDALVKVTSEFSEGYLKW 740
GDANSVY AIV+AP GVSVRVTP NLKFS LNEK+TYSVTFS V+ SEFSEGYL W
Sbjct: 661 GDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIW 720
Query: 741 VSDKHLVRSPISVK 750
VS+KH+VRSPISVK
Sbjct: 721 VSNKHIVRSPISVK 732
BLAST of Lsi09G003440 vs. ExPASy TrEMBL
Match:
A0A5A7THE4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003290 PE=3 SV=1)
HSP 1 Score: 1147.1 bits (2966), Expect = 0.0e+00
Identity = 587/734 (79.97%), Postives = 646/734 (88.01%), Query Frame = 0
Query: 21 MKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYTSFLPKTIE-TSNEQSRLLYS 80
MKT +S TSNT LQTYIVHV+QP LE DT DL++WYTSFLP+TIE +S+EQ RLLYS
Sbjct: 1 MKTELSSTSNT-NLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYS 60
Query: 81 YRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNF 140
YRHVMSGFSARLT+EQVK+MEEKDGFISA PET LNLHTTHTPEYLGLN FGLWK+SNF
Sbjct: 61 YRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNF 120
Query: 141 GKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNLANTVL 200
GKGVIIGVLDTGI+P+HPSFND+GM PPAKWKGRCEFGASICNNKLIGARTFNLAN +
Sbjct: 121 GKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFV 180
Query: 201 MGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLAHVAMYKVCS-KGCQSS 260
+G++P DENGHGTHTASTAAGTFV+GAEALGNARGKAVG+APLAH+AMYKVCS GC SS
Sbjct: 181 IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSS 240
Query: 261 DILAGLDAAIDDGVDVLSISIGSGSGYSLVDDDVAIGAFAAIKKGIFVSCSAGNSGPSKS 320
DILA LDAAIDDGVDVLS+S+G+ S D +AIGAFAAIKKGIFVSCSAGNSGPSK+
Sbjct: 241 DILAALDAAIDDGVDVLSLSLGAPS-TPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKN 300
Query: 321 TLANEAPWILTVGASTIDRTIAALAKLGNDEVHVGESLFQPRDFTSTFLPLVYAGNSGTK 380
TLANEAPWILTVGASTIDR I ALAKLG+ EV+ GESLFQPR+F S FLPLVYAG SG +
Sbjct: 301 TLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIE 360
Query: 381 RSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAAMILINQKPNGFTTSAD 440
SE+C+QGSL L+V GKVVVCE GG I RV KGLVVKN GGAAMILINQKP GF+T A+
Sbjct: 361 GSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAE 420
Query: 441 AHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTDFSPAMASFSSRGPCLS 500
AHVLP T +SYE GLKIKAYI+SS NP ASISFKGT++G T FSPAMASFSSRGPC +
Sbjct: 421 AHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQA 480
Query: 501 SHGILKPDITGPGVNILAAWPFPI--DSNKNTKSTFNVMSGTSMSCPHLSGIAALIKNVH 560
S GILKPDITGPGVNILAAWPFP+ ++N NTKSTFNV+SGTSMSCPHLSGIAALIK+ H
Sbjct: 481 SPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYH 540
Query: 561 PNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGSGHVNPSKATDPGLVYDIQA 620
PNWSPAAIKSAIMTSADV+ PQGKPI+DQDLKPANFFA GSGHVNPSKA +PGLVYDIQ
Sbjct: 541 PNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQP 600
Query: 621 DDYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYPSFSVTLGA-SQTFSRTVTNV 680
DDY+ YLC LYTD QVS+IVR+Q+TCSTV +IREGDLNYPSF+V+L A SQTF+RTVTNV
Sbjct: 601 DDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNV 660
Query: 681 GDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDALVKVTSEFSEGYLKW 740
GDANSVY IVEAP GVSV+VTPSNLKFS LNEK+TYSVTFS V+ TSEFSEGYL W
Sbjct: 661 GDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIW 720
Query: 741 VSDKHLVRSPISVK 750
VS K +VRSPISVK
Sbjct: 721 VSKKLMVRSPISVK 732
BLAST of Lsi09G003440 vs. ExPASy TrEMBL
Match:
A0A5A7TMM4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003280 PE=3 SV=1)
HSP 1 Score: 1075.1 bits (2779), Expect = 0.0e+00
Identity = 546/757 (72.13%), Postives = 633/757 (83.62%), Query Frame = 0
Query: 1 MVVLPLFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPEL----ESSDDTFDLE 60
MVVL IFFLLN +VA K + T KLQTYIVHV+QP++ ESS+D +E
Sbjct: 1 MVVLRFLSIFFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSND--HIE 60
Query: 61 SWYTSFLPKTIETSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNL 120
SWY SF+PK+ ET+ EQ +LLYSYR+VMSGFSARLT EQVK+ME+KDGFISA PETI++L
Sbjct: 61 SWYMSFIPKSTETTVEQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSL 120
Query: 121 HTTHTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCE 180
HTTHTPEYLGLNQQFG+WK+SNFGKGVI+GVLDTGI+P+HPSFND+GM PPAKWKG+CE
Sbjct: 121 HTTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCE 180
Query: 181 FGASICNNKLIGARTFNLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKA 240
F +S+CNNKLIGARTFNL N LM E+P DE GHGTHTASTAAG FVE AEALGNA+GKA
Sbjct: 181 FNSSLCNNKLIGARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKA 240
Query: 241 VGIAPLAHVAMYKVCS-KGCQSSDILAGLDAAIDDGVDVLSISIGSGSGYSLVDDDVAIG 300
GIAPLAH+A+YKVCS K C SSD+ AG+DAAIDDGVDVLSIS+GS S D++A+
Sbjct: 241 AGIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRS-VPFFKDNIAVA 300
Query: 301 AFAAIKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIAALAKLGNDEVHVGES 360
F AI+KGIFVS SAGNSGP STL+N+APWILTVGASTI+R I A+AKLGN E + GES
Sbjct: 301 TFGAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGES 360
Query: 361 LFQPRDFTSTFLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVV 420
L+QP DF S FLPLVYAGN K FC +GSL N+DVKGKVVVCE G +GRV KGLVV
Sbjct: 361 LYQPNDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVV 420
Query: 421 KNAGGAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTI 480
KNAGGAAMILINQ+ +GF+T ++AHVLPAT VSY+AG+ IK+YI+SSQNPTASISFKGT+
Sbjct: 421 KNAGGAAMILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTV 480
Query: 481 IGGSNTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPIDSNKNTKSTFNVM 540
IG + +P+MASFSSRGPCL S GILKPDITGPGVNILAAWPFP+D++ NTKSTFNV+
Sbjct: 481 IGDGDDFSAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNDTNTKSTFNVI 540
Query: 541 SGTSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFAT 600
SGTSMSCPHLSGIAALIK+VHPNWSPAAIKSAIMT+A++KTPQG+PIVDQDL+PANFFA
Sbjct: 541 SGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPIVDQDLQPANFFAM 600
Query: 601 GSGHVNPSKATDPGLVYDIQADDYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNY 660
G+GHVNPSKA DPGLVYDIQ DDY+ YLCGLY D +VS+IV R + C V IREGDLNY
Sbjct: 601 GAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVPSIREGDLNY 660
Query: 661 PSFSVTLGASQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVT 720
PSFSV LG QTF RTVTNVG+ANSVY+AIVEAP+GVS+ V P L FS +N+ +T++VT
Sbjct: 661 PSFSVALGGLQTFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVT 720
Query: 721 FSVDALVKVTSEFSEGYLKWV--SDKHLVRSPISVKF 751
F+ VK+ EF EGYLKWV S K++VRSP+SVKF
Sbjct: 721 FNRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPVSVKF 754
BLAST of Lsi09G003440 vs. ExPASy TrEMBL
Match:
A0A1S3AUP1 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103483066 PE=3 SV=1)
HSP 1 Score: 1068.1 bits (2761), Expect = 1.6e-308
Identity = 542/753 (71.98%), Postives = 630/753 (83.67%), Query Frame = 0
Query: 5 PLFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPEL----ESSDDTFDLESWYT 64
PL+ FFLLN +VA K + T KLQTYIVHV+QP++ ESS+D +ESWY
Sbjct: 16 PLY--FFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSND--HIESWYM 75
Query: 65 SFLPKTIETSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTH 124
SF+PK+ ET+ EQ +LLYSYR+VMSGFSARLT EQVK+ME+KDGFISA PETI++LHTTH
Sbjct: 76 SFIPKSTETTVEQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLHTTH 135
Query: 125 TPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGAS 184
TPEYLGLNQQFG+WK+SNFGKGVI+GVLDTGI+P+HPSFND+GM PPAKWKG+CEF +S
Sbjct: 136 TPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEFNSS 195
Query: 185 ICNNKLIGARTFNLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIA 244
+CNNKLIGARTFNL N LM E+P DE GHGTHTASTAAG FVE AEALGNA+GKA GIA
Sbjct: 196 LCNNKLIGARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAAGIA 255
Query: 245 PLAHVAMYKVCS-KGCQSSDILAGLDAAIDDGVDVLSISIGSGSGYSLVDDDVAIGAFAA 304
PLAH+A+YKVCS K C SSD+ AG+DAAIDDGVDVLSIS+GS S D++A+ F A
Sbjct: 256 PLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRS-VPFFKDNIAVATFGA 315
Query: 305 IKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIAALAKLGNDEVHVGESLFQP 364
I+KGIFVS SAGNSGP STL+N+APWILTVGASTI+R I A+AKLGN E + GESL+QP
Sbjct: 316 IQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLYQP 375
Query: 365 RDFTSTFLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAG 424
DF S FLPLVYAGN K FC +GSL N+DVKGKVVVCE G +GRV KGLVVKNAG
Sbjct: 376 NDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVKNAG 435
Query: 425 GAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGS 484
GAAMILINQ+ +GF+T ++AHVLPAT VSY+AG+ IK+YI+SSQNPTASISFKGT+IG
Sbjct: 436 GAAMILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVIGDG 495
Query: 485 NTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPIDSNKNTKSTFNVMSGTS 544
+ +P+MASFSSRGPCL S GILKPDITGPGVNILAAWPFP+D++ NTKSTFNV+SGTS
Sbjct: 496 DDFSAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNDTNTKSTFNVISGTS 555
Query: 545 MSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGSGH 604
MSCPHLSGIAALIK+VHPNWSPAAIKSAIMT+A++KTPQG+PI DQDL+PANFFA G+GH
Sbjct: 556 MSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPITDQDLQPANFFAMGAGH 615
Query: 605 VNPSKATDPGLVYDIQADDYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYPSFS 664
VNPSKA DPGLVYDIQ DDY+ YLCGLY D +VS+IV R + C V IREGDLNYPSFS
Sbjct: 616 VNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVLSIREGDLNYPSFS 675
Query: 665 VTLGASQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVD 724
V LG QTF RTVTNVG+ANSVY+AIVEAP+GVS+ V P L FS +N+ +T++VTF+
Sbjct: 676 VALGGLQTFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVTFNRI 735
Query: 725 ALVKVTSEFSEGYLKWV--SDKHLVRSPISVKF 751
VK+ EF EGYLKWV S K++VRSP+SVKF
Sbjct: 736 RWVKIVGEFGEGYLKWVSKSKKYVVRSPVSVKF 763
BLAST of Lsi09G003440 vs. NCBI nr
Match:
XP_038906452.1 (subtilisin-like protease 4 [Benincasa hispida])
HSP 1 Score: 1208.7 bits (3126), Expect = 0.0e+00
Identity = 608/732 (83.06%), Postives = 668/732 (91.26%), Query Frame = 0
Query: 21 MKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYTSFLPKTIETSNEQSRLLYSY 80
MK+ +S TSN IKLQTYIVHV++ EL D DLESWYTSFLP+TI+TSNEQSRLLYS+
Sbjct: 1 MKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPETIKTSNEQSRLLYSF 60
Query: 81 RHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFG 140
R+VMSGFSARLTEE VK+MEEK+GFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFG
Sbjct: 61 RNVMSGFSARLTEE-VKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFG 120
Query: 141 KGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNLANTVLM 200
KGVIIGVLDTGI+PDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFN AN+ LM
Sbjct: 121 KGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNRANSALM 180
Query: 201 GETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLAHVAMYKVCS-KGCQSSD 260
GE+PIDENGHGTHTASTAAGTFVEGAEALGNARGKAVG+APLAH+AMYKVCS KGC SSD
Sbjct: 181 GESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSSSD 240
Query: 261 ILAGLDAAIDDGVDVLSISIGSGSGYSLVDDDVAIGAFAAIKKGIFVSCSAGNSGPSKST 320
ILA LDAAI DGVDVLS+S+G+ S + D++AIGAFAAIK GIFVSCSAGNSGPSKST
Sbjct: 241 ILAALDAAIHDGVDVLSLSLGAPSRPFYI-DNIAIGAFAAIKMGIFVSCSAGNSGPSKST 300
Query: 321 LANEAPWILTVGASTIDRTIAALAKLGNDEVHVGESLFQPRDFTSTFLPLVYAGNSGTKR 380
LANEAPWILTVGASTIDRTI ALA+LGN EVHVGESLFQPRDF S+FLPLVYAG+SG K
Sbjct: 301 LANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVKG 360
Query: 381 SEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAAMILINQKPNGFTTSADA 440
SEFCVQGSL NL+VKGK+VVCE GG IGR+ KG+VVKNAGGAAMI+INQK + F+T A+A
Sbjct: 361 SEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAEA 420
Query: 441 HVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTDFSPAMASFSSRGPCLSS 500
H+LPAT VSYEAGLKIK+YI SSQNP ASISF+GT+IG T FSPAMASFSSRGPCL S
Sbjct: 421 HILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFSSRGPCLPS 480
Query: 501 HGILKPDITGPGVNILAAWPFPIDSNKNTKSTFNVMSGTSMSCPHLSGIAALIKNVHPNW 560
GILKPDITGPGVNILAAWPF +D+N N KSTFNV+SGTSMSCPHLSGIAALIK+VHPNW
Sbjct: 481 PGILKPDITGPGVNILAAWPFSLDNNTNKKSTFNVISGTSMSCPHLSGIAALIKSVHPNW 540
Query: 561 SPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGSGHVNPSKATDPGLVYDIQADDY 620
SPAAIKSAIMTSADV+ QG+PIVDQDLKPANFFA GSGHVNPSKA DPGLVYDIQ DDY
Sbjct: 541 SPAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQPDDY 600
Query: 621 VRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYPSFSVTLGASQTFSRTVTNVGDAN 680
+ YLCGLY+D QVS+IVRR++TCST+S+IREGDLNYPSF+VTLG SQ F+RTVTNVGDAN
Sbjct: 601 IPYLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTLGPSQMFNRTVTNVGDAN 660
Query: 681 SVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDALVKVTSEFSEGYLKWVSDK 740
S+YSAIVEAP+GVSV+VTP+NLKFS + EK+TYSVTFS D LV+ TSEFSEGYL WVS+K
Sbjct: 661 SIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVRTTSEFSEGYLIWVSNK 720
Query: 741 HLVRSPISVKFK 752
H+VRSPISVK K
Sbjct: 721 HMVRSPISVKLK 730
BLAST of Lsi09G003440 vs. NCBI nr
Match:
XP_004147599.2 (subtilisin-like protease SBT1.7 [Cucumis sativus] >KAE8647878.1 hypothetical protein Csa_000520 [Cucumis sativus])
HSP 1 Score: 1182.2 bits (3057), Expect = 0.0e+00
Identity = 602/754 (79.84%), Postives = 666/754 (88.33%), Query Frame = 0
Query: 1 MVVLPLFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYT 60
MV LP CIFF LNFHG VAMKT + SNT LQTYIVHV+QPE+E DT DL++WYT
Sbjct: 1 MVFLPFICIFFFLNFHGYVAMKTELWSASNT-NLQTYIVHVKQPEVEILGDTIDLQNWYT 60
Query: 61 SFLPKTIE-TSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTT 120
SFLP+TIE +SNEQSRLLYSYRHV+SGFSARLT+EQVK+MEEKDGFISA PET LNLHTT
Sbjct: 61 SFLPETIEASSNEQSRLLYSYRHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTT 120
Query: 121 HTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGA 180
HTPEYLGLNQ FGLWK+SNFGKGVIIGVLDTGI+P+HPSFND+GMP PPAKWKGRCEFGA
Sbjct: 121 HTPEYLGLNQHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGA 180
Query: 181 SICNNKLIGARTFNLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGI 240
SICNNKLIGARTFNLAN V +G++P DENGHGTHTASTAAGTFV+GAEALGNARGKAVG+
Sbjct: 181 SICNNKLIGARTFNLANNVSIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGM 240
Query: 241 APLAHVAMYKVCS-KGCQSSDILAGLDAAIDDGVDVLSISIGSGSGYSLVDDDVAIGAFA 300
APLAH+A+YKVCS KGC SSDILA LDAAIDDGVDVLS+S+G+ S D +A+GAFA
Sbjct: 241 APLAHIAVYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGAPS-TPFFKDTIAVGAFA 300
Query: 301 AIKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIAALAKLGNDEVHVGESLFQ 360
AIKKGIFVSCSAGNSGPSK+TLANEAPWILTVGASTIDR I ALAKL + +V GESLFQ
Sbjct: 301 AIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQ 360
Query: 361 PRDFTSTFLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNA 420
PRDF+S FLPLVYAG SG + SE+CV+GSL L+V GK+VVCE GG IGR+ KGLVVKN
Sbjct: 361 PRDFSSKFLPLVYAGKSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNG 420
Query: 421 GGAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGG 480
GGAAMIL+NQKP+GF+T A+AHVLP T +SYE GLKIK YI+SS NP ASISF+GT++G
Sbjct: 421 GGAAMILVNQKPDGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGN 480
Query: 481 SNTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPI--DSNKNTKSTFNVMS 540
T FSPAMASFSSRGPC +S GILKPDITGPGVNILAAWPFP+ ++N NTKSTFNV+S
Sbjct: 481 RATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVIS 540
Query: 541 GTSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATG 600
GTSMSCPHLSGIAALIK+ HPNWSPAAIKSAIMTSADV+ PQGKPIVDQDLKPANFFA G
Sbjct: 541 GTSMSCPHLSGIAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMG 600
Query: 601 SGHVNPSKATDPGLVYDIQADDYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYP 660
SGHVNPSKA +PGLVYDIQ DDYV YLC LYTD QVS+IVRRQ+TCSTVS+IREGDLNYP
Sbjct: 601 SGHVNPSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYP 660
Query: 661 SFSVTLGA-SQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVT 720
SF+V+LGA SQ F+RTVTNVGDANSVY AIV+AP GVSVRVTP NLKFS LNEK+TYSVT
Sbjct: 661 SFAVSLGADSQAFNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVT 720
Query: 721 FSVDALVKVTSEFSEGYLKWVSDKHLVRSPISVK 750
FS V+ SEFSEGYL WVS+KH+VRSPISVK
Sbjct: 721 FSRIDFVRTRSEFSEGYLIWVSNKHIVRSPISVK 752
BLAST of Lsi09G003440 vs. NCBI nr
Match:
XP_008437177.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])
HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 602/754 (79.84%), Postives = 663/754 (87.93%), Query Frame = 0
Query: 1 MVVLPLFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYT 60
MV LP CIFF LNFHG VAMKT +S TSNT LQTYIVHV+QP LE DT DL++WYT
Sbjct: 1 MVFLPFICIFFFLNFHGYVAMKTELSSTSNT-NLQTYIVHVKQPVLEILGDTIDLQNWYT 60
Query: 61 SFLPKTIE-TSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTT 120
SFLP+TIE +S+EQ RLLYSYRHVMSGFSARLT+EQVK+MEEKDGFISA PET LNLHTT
Sbjct: 61 SFLPETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTT 120
Query: 121 HTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGA 180
HTPEYLGLN+ FGLWK+SNFGKGVIIGVLDTGI+P+HPSFND+GM PPAKWKGRCEFGA
Sbjct: 121 HTPEYLGLNKHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGA 180
Query: 181 SICNNKLIGARTFNLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGI 240
SICNNKLIGARTFNLAN ++G++P DENGHGTHTASTAAGTFV+GAEALGNARGKAVG+
Sbjct: 181 SICNNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGM 240
Query: 241 APLAHVAMYKVCS-KGCQSSDILAGLDAAIDDGVDVLSISIGSGSGYSLVDDDVAIGAFA 300
APLAH+AMYKVCS GC SSDILA LDAAIDDGVDVLS+S+G+ S D +A+GAFA
Sbjct: 241 APLAHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPS-TPFFRDTIAMGAFA 300
Query: 301 AIKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIAALAKLGNDEVHVGESLFQ 360
AIKKGIFVSCSAGNSGPSK+TLANEAPWILTVGASTIDR I ALAKLG+ EV+ GESLFQ
Sbjct: 301 AIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQ 360
Query: 361 PRDFTSTFLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNA 420
PR+F S FLPLVYAG SG + SE+C+QGSL L+V GKVVVCE GG I RV KGLVVKN
Sbjct: 361 PRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNG 420
Query: 421 GGAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGG 480
GGAAMILINQKP GF+T A+AHVLP T +SYE GLKIKAYI+SS NP ASISFKGT++G
Sbjct: 421 GGAAMILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGN 480
Query: 481 SNTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPI--DSNKNTKSTFNVMS 540
T FSPAMASFSSRGPC +S GILKPDITGPGVNILAAWPFP+ ++N NTKSTFNV+S
Sbjct: 481 RATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVIS 540
Query: 541 GTSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATG 600
GTSMSCPHLSGIAALIK+ HPNWSPAAIKSAIMTSADV+ PQGKPI+DQDLKPANFFA G
Sbjct: 541 GTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMG 600
Query: 601 SGHVNPSKATDPGLVYDIQADDYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYP 660
SGHVNPSKA +PGLVYDIQ DDY+ YLC LYTD QVS+IVR+Q+TCSTV +IREGDLNYP
Sbjct: 601 SGHVNPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYP 660
Query: 661 SFSVTLGA-SQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVT 720
SF+V+LGA SQTF+RTVTNVGDANSVY IVEAP GVSV+VTPSNLKFS LNEK+TYSVT
Sbjct: 661 SFAVSLGAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVT 720
Query: 721 FSVDALVKVTSEFSEGYLKWVSDKHLVRSPISVK 750
FS V+ TSEFSEGYL WVS K +VRSPISVK
Sbjct: 721 FSRIDFVRTTSEFSEGYLIWVSKKLMVRSPISVK 752
BLAST of Lsi09G003440 vs. NCBI nr
Match:
KAA0042822.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])
HSP 1 Score: 1147.1 bits (2966), Expect = 0.0e+00
Identity = 587/734 (79.97%), Postives = 646/734 (88.01%), Query Frame = 0
Query: 21 MKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYTSFLPKTIE-TSNEQSRLLYS 80
MKT +S TSNT LQTYIVHV+QP LE DT DL++WYTSFLP+TIE +S+EQ RLLYS
Sbjct: 1 MKTELSSTSNT-NLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYS 60
Query: 81 YRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNF 140
YRHVMSGFSARLT+EQVK+MEEKDGFISA PET LNLHTTHTPEYLGLN FGLWK+SNF
Sbjct: 61 YRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNF 120
Query: 141 GKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNLANTVL 200
GKGVIIGVLDTGI+P+HPSFND+GM PPAKWKGRCEFGASICNNKLIGARTFNLAN +
Sbjct: 121 GKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFV 180
Query: 201 MGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLAHVAMYKVCS-KGCQSS 260
+G++P DENGHGTHTASTAAGTFV+GAEALGNARGKAVG+APLAH+AMYKVCS GC SS
Sbjct: 181 IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSS 240
Query: 261 DILAGLDAAIDDGVDVLSISIGSGSGYSLVDDDVAIGAFAAIKKGIFVSCSAGNSGPSKS 320
DILA LDAAIDDGVDVLS+S+G+ S D +AIGAFAAIKKGIFVSCSAGNSGPSK+
Sbjct: 241 DILAALDAAIDDGVDVLSLSLGAPS-TPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKN 300
Query: 321 TLANEAPWILTVGASTIDRTIAALAKLGNDEVHVGESLFQPRDFTSTFLPLVYAGNSGTK 380
TLANEAPWILTVGASTIDR I ALAKLG+ EV+ GESLFQPR+F S FLPLVYAG SG +
Sbjct: 301 TLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIE 360
Query: 381 RSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAAMILINQKPNGFTTSAD 440
SE+C+QGSL L+V GKVVVCE GG I RV KGLVVKN GGAAMILINQKP GF+T A+
Sbjct: 361 GSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAE 420
Query: 441 AHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTDFSPAMASFSSRGPCLS 500
AHVLP T +SYE GLKIKAYI+SS NP ASISFKGT++G T FSPAMASFSSRGPC +
Sbjct: 421 AHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQA 480
Query: 501 SHGILKPDITGPGVNILAAWPFPI--DSNKNTKSTFNVMSGTSMSCPHLSGIAALIKNVH 560
S GILKPDITGPGVNILAAWPFP+ ++N NTKSTFNV+SGTSMSCPHLSGIAALIK+ H
Sbjct: 481 SPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYH 540
Query: 561 PNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFATGSGHVNPSKATDPGLVYDIQA 620
PNWSPAAIKSAIMTSADV+ PQGKPI+DQDLKPANFFA GSGHVNPSKA +PGLVYDIQ
Sbjct: 541 PNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQP 600
Query: 621 DDYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNYPSFSVTLGA-SQTFSRTVTNV 680
DDY+ YLC LYTD QVS+IVR+Q+TCSTV +IREGDLNYPSF+V+L A SQTF+RTVTNV
Sbjct: 601 DDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNV 660
Query: 681 GDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDALVKVTSEFSEGYLKW 740
GDANSVY IVEAP GVSV+VTPSNLKFS LNEK+TYSVTFS V+ TSEFSEGYL W
Sbjct: 661 GDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIW 720
Query: 741 VSDKHLVRSPISVK 750
VS K +VRSPISVK
Sbjct: 721 VSKKLMVRSPISVK 732
BLAST of Lsi09G003440 vs. NCBI nr
Match:
KAA0042821.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])
HSP 1 Score: 1075.1 bits (2779), Expect = 0.0e+00
Identity = 546/757 (72.13%), Postives = 633/757 (83.62%), Query Frame = 0
Query: 1 MVVLPLFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPEL----ESSDDTFDLE 60
MVVL IFFLLN +VA K + T KLQTYIVHV+QP++ ESS+D +E
Sbjct: 1 MVVLRFLSIFFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSND--HIE 60
Query: 61 SWYTSFLPKTIETSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNL 120
SWY SF+PK+ ET+ EQ +LLYSYR+VMSGFSARLT EQVK+ME+KDGFISA PETI++L
Sbjct: 61 SWYMSFIPKSTETTVEQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSL 120
Query: 121 HTTHTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCE 180
HTTHTPEYLGLNQQFG+WK+SNFGKGVI+GVLDTGI+P+HPSFND+GM PPAKWKG+CE
Sbjct: 121 HTTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCE 180
Query: 181 FGASICNNKLIGARTFNLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKA 240
F +S+CNNKLIGARTFNL N LM E+P DE GHGTHTASTAAG FVE AEALGNA+GKA
Sbjct: 181 FNSSLCNNKLIGARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKA 240
Query: 241 VGIAPLAHVAMYKVCS-KGCQSSDILAGLDAAIDDGVDVLSISIGSGSGYSLVDDDVAIG 300
GIAPLAH+A+YKVCS K C SSD+ AG+DAAIDDGVDVLSIS+GS S D++A+
Sbjct: 241 AGIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRS-VPFFKDNIAVA 300
Query: 301 AFAAIKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIAALAKLGNDEVHVGES 360
F AI+KGIFVS SAGNSGP STL+N+APWILTVGASTI+R I A+AKLGN E + GES
Sbjct: 301 TFGAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGES 360
Query: 361 LFQPRDFTSTFLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVV 420
L+QP DF S FLPLVYAGN K FC +GSL N+DVKGKVVVCE G +GRV KGLVV
Sbjct: 361 LYQPNDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVV 420
Query: 421 KNAGGAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTI 480
KNAGGAAMILINQ+ +GF+T ++AHVLPAT VSY+AG+ IK+YI+SSQNPTASISFKGT+
Sbjct: 421 KNAGGAAMILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTV 480
Query: 481 IGGSNTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAWPFPIDSNKNTKSTFNVM 540
IG + +P+MASFSSRGPCL S GILKPDITGPGVNILAAWPFP+D++ NTKSTFNV+
Sbjct: 481 IGDGDDFSAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNDTNTKSTFNVI 540
Query: 541 SGTSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDLKPANFFAT 600
SGTSMSCPHLSGIAALIK+VHPNWSPAAIKSAIMT+A++KTPQG+PIVDQDL+PANFFA
Sbjct: 541 SGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPIVDQDLQPANFFAM 600
Query: 601 GSGHVNPSKATDPGLVYDIQADDYVRYLCGLYTDVQVSMIVRRQITCSTVSKIREGDLNY 660
G+GHVNPSKA DPGLVYDIQ DDY+ YLCGLY D +VS+IV R + C V IREGDLNY
Sbjct: 601 GAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVPSIREGDLNY 660
Query: 661 PSFSVTLGASQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVT 720
PSFSV LG QTF RTVTNVG+ANSVY+AIVEAP+GVS+ V P L FS +N+ +T++VT
Sbjct: 661 PSFSVALGGLQTFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVT 720
Query: 721 FSVDALVKVTSEFSEGYLKWV--SDKHLVRSPISVKF 751
F+ VK+ EF EGYLKWV S K++VRSP+SVKF
Sbjct: 721 FNRIRWVKIVGEFGEGYLKWVSKSKKYVVRSPVSVKF 754
BLAST of Lsi09G003440 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 604.4 bits (1557), Expect = 1.3e-172
Identity = 358/766 (46.74%), Postives = 481/766 (62.79%), Query Frame = 0
Query: 10 FFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLES-WYTSFLPKTIE 69
FFLL G + ++ SD TYIVH+ + ++ SS FDL S WY S ++
Sbjct: 11 FFLLLCLGFCHVSSSSSDQG------TYIVHMAKSQMPSS---FDLHSNWYDS----SLR 70
Query: 70 TSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYLGLN 129
+ ++ + LLY+Y + + GFS RLT+E+ S+ + G IS PE LHTT TP +LGL+
Sbjct: 71 SISDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD 130
Query: 130 QQFG-LWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCE----FGASICN 189
+ L+ ++ V++GVLDTG+ P+ S++D+G P+ WKG CE F AS+CN
Sbjct: 131 EHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCN 190
Query: 190 NKLIGARTFNLANTVLMG--------ETPIDENGHGTHTASTAAGTFVEGAEALGNARGK 249
KLIGAR F MG +P D++GHGTHT+STAAG+ VEGA LG A G
Sbjct: 191 RKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGT 250
Query: 250 AVGIAPLAHVAMYKVC-SKGCQSSDILAGLDAAIDDGVDVLSISIGSGSGYSLVDDDVAI 309
A G+AP A VA+YKVC GC SSDILA +D AI D V+VLS+S+G G D VAI
Sbjct: 251 ARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMS-DYYRDGVAI 310
Query: 310 GAFAAIKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIAALAKLGNDEVHVGE 369
GAFAA+++GI VSCSAGN+GPS S+L+N APWI TVGA T+DR ALA LGN + G
Sbjct: 311 GAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGV 370
Query: 370 SLFQPRDFTSTFLPLVYAGN-SGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGL 429
SLF+ LP +YAGN S C+ G+L VKGK+V+C+ G N RV+KG
Sbjct: 371 SLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGD 430
Query: 430 VVKNAGGAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKG 489
VVK AGG MIL N NG ADAH+LPAT V +AG I+ Y+++ NPTASIS G
Sbjct: 431 VVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILG 490
Query: 490 TIIGGSNTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAW-----PFPIDSNKNT 549
T++G SP +A+FSSRGP + ILKPD+ PGVNILAAW P + S+ +
Sbjct: 491 TVVG---VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASD-SR 550
Query: 550 KSTFNVMSGTSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVD-QDL 609
+ FN++SGTSMSCPH+SG+AAL+K+VHP WSPAAI+SA+MT+A GKP++D
Sbjct: 551 RVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATG 610
Query: 610 KPANFFATGSGHVNPSKATDPGLVYDIQADDYVRYLCGL-YTDVQVSMIVRRQITCSTVS 669
KP+ F G+GHV+P+ AT+PGL+YD+ +DY+ +LC L YT Q+ + RR TC
Sbjct: 611 KPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSK 670
Query: 670 KIREGDLNYPSFSVTLG--ASQTFSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFS 729
DLNYPSF+V + + ++RTVT+VG A + + GV + V P+ L F
Sbjct: 671 SYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFK 730
Query: 730 SLNEKITYSVTFSVDALVKVTSEFSEGYLKWVSDKHLVRSPISVKF 751
NEK +Y+VTF+VD+ K + S G ++W KH+V SP+++ +
Sbjct: 731 EANEKKSYTVTFTVDS-SKPSGSNSFGSIEWSDGKHVVGSPVAISW 756
BLAST of Lsi09G003440 vs. TAIR 10
Match:
AT1G04110.1 (Subtilase family protein )
HSP 1 Score: 594.0 bits (1530), Expect = 1.7e-169
Identity = 355/790 (44.94%), Postives = 483/790 (61.14%), Query Frame = 0
Query: 5 PLF-CIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYTSFL 64
P F CI FLL S +S ++ QTYIV + P E++ W+ SFL
Sbjct: 5 PFFLCIIFLL----------FCSSSSEILQKQTYIVQL-HPNSETAKTFASKFDWHLSFL 64
Query: 65 PKTI-----ETSNEQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHT 124
+ + E SRLLYSY + GF+A+LTE + + + ++ RP+ +L + T
Sbjct: 65 QEAVLGVEEEEEEPSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQT 124
Query: 125 THTPEYLGLN--QQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCE 184
T++ ++LGL+ G+W S FG+G IIGVLDTG+ P+ PSF+D GMP P KWKG C+
Sbjct: 125 TYSYKFLGLDGFGNSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQ 184
Query: 185 ----FGASICNNKLIGARTFNLANTVLMG--ETP---------IDENGHGTHTASTAAGT 244
F +S CN KLIGAR F + V E+P D GHGTHTAST G+
Sbjct: 185 EGESFSSSSCNRKLIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGS 244
Query: 245 FVEGAEALGNARGKAVGIAPLAHVAMYKVC-SKGCQSSDILAGLDAAIDDGVDVLSISIG 304
V A LGN G A G+AP AH+A+YKVC GC SSDILA +D AI D VDVLS+S+G
Sbjct: 245 SVSMANVLGNGAGVARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLG 304
Query: 305 SGSGYSLVDDDVAIGAFAAIKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIA 364
G L DD +AIG F A+++GI V C+AGN+GP +S++AN APW+ T+GA T+DR
Sbjct: 305 -GFPIPLYDDTIAIGTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFP 364
Query: 365 ALAKLGNDEVHVGESLFQPRDFTST--FLPLVYAGNSGTKRSEFCVQGSLANLDVKGKVV 424
A+ +L N ++ GESL+ + + + ++Y G K SEFC++GSL +++GK+V
Sbjct: 365 AVVRLANGKLLYGESLYPGKGIKNAGREVEVIYV-TGGDKGSEFCLRGSLPREEIRGKMV 424
Query: 425 VCETGGNIGRVKKGLVVKNAGGAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAY 484
+C+ G N GR +KG VK AGG AMIL N + N S D H+LPAT + Y + +KAY
Sbjct: 425 ICDRGVN-GRSEKGEAVKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAY 484
Query: 485 ISSSQNPTASISFKGTIIGGSNTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAW 544
++++ P A I F GT+IG S +P +A FS+RGP L++ ILKPD+ PGVNI+AAW
Sbjct: 485 VNATVKPKARIIFGGTVIGRSR---APEVAQFSARGPSLANPSILKPDMIAPGVNIIAAW 544
Query: 545 P-------FPIDSNKNTKSTFNVMSGTSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTS 604
P P DS + F VMSGTSMSCPH+SGI ALI++ +PNWSPAAIKSA+MT+
Sbjct: 545 PQNLGPTGLPYDSR---RVNFTVMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTT 604
Query: 605 ADVKTPQGKPIVDQDLKPANFFATGSGHVNPSKATDPGLVYDIQADDYVRYLCGL-YTDV 664
AD+ QGK I D + KPA FA G+GHVNP KA +PGLVY+IQ DY+ YLC L +T
Sbjct: 605 ADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRS 664
Query: 665 QVSMIVRRQITCSTVSKIREG-DLNYPSFSVTLGASQT---FSRTVTNVGDANSVYSAIV 724
+ I + ++C+ + + G LNYPS +V +T +R VTNVG NS+YS V
Sbjct: 665 DILAITHKNVSCNGILRKNPGFSLNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNV 724
Query: 725 EAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDALVK--VTSEFSEGYLKWVSDKHL--- 752
+AP G+ V V P L F +++ ++Y V F + + + F++G L WV+ +L
Sbjct: 725 KAPEGIKVIVNPKRLVFKHVDQTLSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNLMQR 773
BLAST of Lsi09G003440 vs. TAIR 10
Match:
AT3G14067.1 (Subtilase family protein )
HSP 1 Score: 582.8 bits (1501), Expect = 4.0e-166
Identity = 346/759 (45.59%), Postives = 466/759 (61.40%), Query Frame = 0
Query: 28 TSNTIKLQTYIVHVQ---QPELESSDDTFDLESWYTSFLPKTIETSNEQSRLLYSYRHVM 87
+S++ L++YIVHVQ +P L SS + +W+ S L +++ +S + + LLYSY +
Sbjct: 24 SSSSDGLESYIVHVQRSHKPSLFSSHN-----NWHVSLL-RSLPSSPQPATLLYSYSRAV 83
Query: 88 SGFSARLTEEQVKSMEEKDGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGKGVI 147
GFSARL+ Q ++ IS P+ +HTTHTP +LG +Q GLW +SN+G+ VI
Sbjct: 84 HGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVI 143
Query: 148 IGVLDTGINPDHPSFNDDGMPQPPAKWKGRCEFG----ASICNNKLIGARTF-------- 207
+GVLDTGI P+HPSF+D G+ P+ WKG CE G AS CN KLIGAR F
Sbjct: 144 VGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQR 203
Query: 208 --NLANTVLMGETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLAHVAMYKV 267
+ +P D GHGTHTASTAAG+ V A ARG A G+A A +A YK+
Sbjct: 204 NGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKI 263
Query: 268 C-SKGCQSSDILAGLDAAIDDGVDVLSISIG-SGSGYSLVDDDVAIGAFAAIKKGIFVSC 327
C + GC SDILA +D A+ DGV V+S+S+G SGS D +AIGAF A + GI VSC
Sbjct: 264 CWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSC 323
Query: 328 SAGNSGPSKSTLANEAPWILTVGASTIDRTIAALAKLGNDEVHVGESLFQPRDFTSTFLP 387
SAGNSGP+ T N APWILTVGAST+DR AA A G+ +V G SL+ + L
Sbjct: 324 SAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLS 383
Query: 388 LVYAGNSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAAMILINQ 447
LVY+G+ G S C G L + V+GK+V+C+ GGN RV+KG VK AGGA MIL N
Sbjct: 384 LVYSGDCG---SRLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANT 443
Query: 448 KPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTDFSPAMA 507
+G +AD+H++PAT V +AG +I+ YI +S +PTA ISF GT+IG S SP +A
Sbjct: 444 AESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPP--SPRVA 503
Query: 508 SFSSRGPCLSSHGILKPDITGPGVNILAAW-----PFPIDSNKNTKSTFNVMSGTSMSCP 567
+FSSRGP + ILKPD+ PGVNILA W P +D + + FN++SGTSMSCP
Sbjct: 504 AFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR-RVQFNIISGTSMSCP 563
Query: 568 HLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVDQDL-KPANFFATGSGHVNP 627
H+SG+AAL++ HP+WSPAAIKSA++T+A G+PI D K +N F G+GHV+P
Sbjct: 564 HVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDP 623
Query: 628 SKATDPGLVYDIQADDYVRYLCGLYTDVQVSMIVRRQIT----CSTVSKIREGDLNYPSF 687
+KA +PGLVYDI+ +YV +LC + + ++ + T C T GDLNYPSF
Sbjct: 624 NKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSF 683
Query: 688 SV---TLGASQTFSRTVTNVG-DANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSV 747
SV + G + R V NVG + ++VY V++P V + V+PS L FS + Y V
Sbjct: 684 SVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEV 743
Query: 748 TFSVDAL---VKVTSEFSEGYLKWVSDKHLVRSPISVKF 751
TF L V G ++W +H+V+SP++V++
Sbjct: 744 TFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769
BLAST of Lsi09G003440 vs. TAIR 10
Match:
AT2G05920.1 (Subtilase family protein )
HSP 1 Score: 572.0 bits (1473), Expect = 7.0e-163
Identity = 343/744 (46.10%), Postives = 459/744 (61.69%), Query Frame = 0
Query: 30 NTIKLQTYIVHVQQPELESSDDTFDLESWYTSFLPKTIETSNEQSRLLYSYRHVMSGFSA 89
+T +TYI+ V + S T WYTS L N +S LLY+Y GFSA
Sbjct: 23 HTTAKKTYIIRVNHSDKPESFLTH--HDWYTSQL-------NSESSLLYTYTTSFHGFSA 82
Query: 90 RLTEEQVKS-MEEKDGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGKGVIIGVL 149
L + S + + + + + LHTT TPE+LGLN +FG+ + GVIIGVL
Sbjct: 83 YLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVL 142
Query: 150 DTGINPDHPSFNDDGMPQPPAKWKGRCE----FGASICNNKLIGARTFNLANTVLMG--- 209
DTG+ P+ SF+D MP+ P+KWKG CE F + +CN KLIGAR+F+ + G
Sbjct: 143 DTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGF 202
Query: 210 ------ETPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGIAPLAHVAMYKVC-SKG 269
+P D +GHGTHT++TAAG+ V A LG A G A G+A A VA YKVC S G
Sbjct: 203 SSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTG 262
Query: 270 CQSSDILAGLDAAIDDGVDVLSISIGSGSGYSLVDDDVAIGAFAAIKKGIFVSCSAGNSG 329
C SDILA +D AI DGVDVLS+S+G GS D +AIGAF+A+++G+FVSCSAGNSG
Sbjct: 263 CFGSDILAAMDRAILDGVDVLSLSLGGGSA-PYYRDTIAIGAFSAMERGVFVSCSAGNSG 322
Query: 330 PSKSTLANEAPWILTVGASTIDRTIAALAKLGNDEVHVGESLFQPRDFTSTFLPLVY-AG 389
P+++++AN APW++TVGA T+DR A A LGN + G SL+ + L LVY G
Sbjct: 323 PTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKG 382
Query: 390 NSGTKRSEFCVQGSLANLDVKGKVVVCETGGNIGRVKKGLVVKNAGGAAMILINQKPNGF 449
NS + S C+ GSL + V+GK+VVC+ G N RV+KG VV++AGG MI+ N +G
Sbjct: 383 NSSS--SNLCLPGSLDSSIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGE 442
Query: 450 TTSADAHVLPATRVSYEAGLKIKAYISSSQNPTASISFKGTIIGGSNTDFSPAMASFSSR 509
AD+H+LPA V + G ++ Y+ S PTA + FKGT++ + SP +A+FSSR
Sbjct: 443 ELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL---DVKPSPVVAAFSSR 502
Query: 510 GPCLSSHGILKPDITGPGVNILAAWP---FPIDSNKNTKST-FNVMSGTSMSCPHLSGIA 569
GP + ILKPD+ GPGVNILA W P +K+++ T FN+MSGTSMSCPH+SG+A
Sbjct: 503 GPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLA 562
Query: 570 ALIKNVHPNWSPAAIKSAIMTSADVKTPQGKPIVD-QDLKPANFFATGSGHVNPSKATDP 629
L+K HP WSP+AIKSA+MT+A V P+ D D +N +A GSGHV+P KA P
Sbjct: 563 GLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSP 622
Query: 630 GLVYDIQADDYVRYLCGL-YT-DVQVSMIVRRQITCSTVSKIRE-GDLNYPSFSVTLGAS 689
GLVYDI ++Y+R+LC L YT D V+++ R + CS K + G LNYPSFSV G
Sbjct: 623 GLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCS--KKFSDPGQLNYPSFSVLFGGK 682
Query: 690 QT--FSRTVTNVGDANSVYSAIVEAPVGVSVRVTPSNLKFSSLNEKITYSVTFSVDALVK 748
+ ++R VTNVG A+SVY V V + V PS L F S+ EK Y+VTF V
Sbjct: 683 RVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVS 742
BLAST of Lsi09G003440 vs. TAIR 10
Match:
AT1G01900.1 (subtilase family protein )
HSP 1 Score: 552.4 bits (1422), Expect = 5.7e-157
Identity = 331/781 (42.38%), Postives = 471/781 (60.31%), Query Frame = 0
Query: 1 MVVLPLFCIFFLLNFHGNVAMKTNVSDTSNTIKLQTYIVHVQQPELESSDDTFDLESWYT 60
M+ F +FF L F +NVS + QTY++H ++ + S +
Sbjct: 18 MMFFRSFIVFFFLIF-----FASNVSS-----RKQTYVIHT-----VTTSTKHIVTSLFN 77
Query: 61 SFLPKTIETSN-EQSRLLYSYRHVMSGFSARLTEEQVKSMEEKDGFISARPETILNLHTT 120
S + I + + Y Y + MSGFSA LT++Q+ +++ GFISA P+ +L+LHTT
Sbjct: 78 SLQTENINDDDFSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTT 137
Query: 121 HTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGINPDHPSFNDDGMPQPPAKWKGRCE--- 180
++ E+LGL GLW +++ VIIG++DTGI+P+H SF D M P++W+G C+
Sbjct: 138 YSHEFLGLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGT 197
Query: 181 -FGASICNNKLIGARTFNLANTVLMGE--------TPIDENGHGTHTASTAAGTFVEGAE 240
F +S CN K+IGA F ++G+ + D GHGTHTASTAAG V A
Sbjct: 198 NFSSSECNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKAN 257
Query: 241 ALGNARGKAVGIAPLAHVAMYKVC-SKGCQSSDILAGLDAAIDDGVDVLSISIGSGSGYS 300
G A+G A G+ + +A YK C + GC S+D++A +D AI DGVDV+S+S+G GS
Sbjct: 258 YFGQAKGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLG-GSSRP 317
Query: 301 LVDDDVAIGAFAAIKKGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIAALAKLG 360
D +AI F A++K IFVSCSAGNSGP+ ST++N APW++TV AS DRT A+ ++G
Sbjct: 318 FYVDPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIG 377
Query: 361 NDEVHVGESLFQPRDFTSTFLPLVYAGNSGTKR-SEFCVQGSLANLDVKGKVVVCETGGN 420
N + VG SL++ + + LPL + +G + + FC++ SL V+GK+V+C G +
Sbjct: 378 NRKSLVGSSLYKGKSLKN--LPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGAS 437
Query: 421 IGRVKKGLVVKNAGGAAMILINQKPNGFTTSADAHVLPATRVSYEAGLKIKAYISSSQNP 480
GR KG VK +GGAAM+L++ + G AD HVLPA + + G + Y++ + N
Sbjct: 438 -GRTAKGEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAANA 497
Query: 481 TASISFKGTIIGGSNTDFSPAMASFSSRGPCLSSHGILKPDITGPGVNILAAW-PFPIDS 540
TAS+ F+GT G + +P +A+FSSRGP ++ I KPDI PG+NILA W PF S
Sbjct: 498 TASVRFRGTAYGAT----APMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPS 557
Query: 541 ---NKNTKSTFNVMSGTSMSCPHLSGIAALIKNVHPNWSPAAIKSAIMTSADVKTPQGKP 600
+ + FN++SGTSM+CPH+SGIAALIK+VH +WSPA IKSAIMT+A + + +P
Sbjct: 558 LLRSDPRRVQFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRP 617
Query: 601 IVDQDL----KPANFFATGSGHVNPSKATDPGLVYDIQADDYVRYLCGL-YTDVQVSMIV 660
I D+ A FA G+G+V+P++A DPGLVYD DY+ YLC L YT ++ +
Sbjct: 618 IGDRGAAGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFS 677
Query: 661 RRQITC-STVSKIREGDLNYPSFSVTL--GA---SQTFSRTVTNVGDANSVYSAIVEAPV 720
TC S + GDLNYPSF+V L GA + + RTVTNVG Y VE P
Sbjct: 678 GTNYTCASNAVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPK 737
Query: 721 GVSVRVTPSNLKFSSLNEKITYSVTFSVDALVKVTSEFSEGYLKWVSDKHLVRSPISVKF 752
GV VRV P LKF E+++Y+VT+ +A + +S S G L W+ DK+ VRSPI+V +
Sbjct: 738 GVKVRVEPKVLKFQKARERLSYTVTYDAEA-SRNSSSSSFGVLVWICDKYNVRSPIAVTW 774
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| A9QY38 | 5.4e-261 | 61.55 | Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1 | [more] |
| A0A0M3R8G2 | 1.3e-238 | 57.73 | Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1 | [more] |
| A9QY39 | 4.8e-233 | 58.16 | Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1 | [more] |
| A9QY40 | 2.5e-229 | 58.46 | Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1 | [more] |
| G7KEU7 | 1.3e-227 | 56.48 | Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A1S3AT16 | 0.0e+00 | 79.84 | subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482683 PE=3 SV=... | [more] |
| A0A0A0KMQ7 | 0.0e+00 | 79.97 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157280 PE=3 SV=1 | [more] |
| A0A5A7THE4 | 0.0e+00 | 79.97 | Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... | [more] |
| A0A5A7TMM4 | 0.0e+00 | 72.13 | Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... | [more] |
| A0A1S3AUP1 | 1.6e-308 | 71.98 | subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103483066 PE=3 SV=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_038906452.1 | 0.0e+00 | 83.06 | subtilisin-like protease 4 [Benincasa hispida] | [more] |
| XP_004147599.2 | 0.0e+00 | 79.84 | subtilisin-like protease SBT1.7 [Cucumis sativus] >KAE8647878.1 hypothetical pro... | [more] |
| XP_008437177.1 | 0.0e+00 | 79.84 | PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo] | [more] |
| KAA0042822.1 | 0.0e+00 | 79.97 | subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa] | [more] |
| KAA0042821.1 | 0.0e+00 | 72.13 | subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa] | [more] |
