Lsi08G003220 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G003220
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	cysteine proteinase inhibitor B
Location	chr08: 8561261 .. 8561659 (-)
RNA-Seq Expression	Lsi08G003220
Synteny	Lsi08G003220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGAAACAAAATTCTCTGTAATAATCGCCGTTGTGGCGGTGGCTTTGGTGGCAGCGGGCGTGGTGGTGGAAGGATACGGCGGAAAAGTAGGCGGAAGAATGGAGATCAAAGACGTGAAGAGGAATGAAGAAGTGCAGCGATTAGGAAGATTCAGCGTGGAGGAGTACAATCGGATGGGAGGCAGCGGCGGAGGAGAGGTGAAATTCGCGGCGGTGATTGCGGCGGAGAGGCAGGTGGTGTCGGGAACGAAGTACTATTTGAGAATTTTGGGGATTCAGAATGGGGAGAGGAAGATTTTTGATTCGGTTGTGATTGTGAAGCCGTGGATTGGTTCTAAGCGCCTTCTGGATTTTTCGGCTTCCAGAGTTTTGAGCACTTCAATTTTTAATTTCTGA

mRNA sequence

ATGGCGGAAACAAAATTCTCTGTAATAATCGCCGTTGTGGCGGTGGCTTTGGTGGCAGCGGGCGTGGTGGTGGAAGGATACGGCGGAAAAGTAGGCGGAAGAATGGAGATCAAAGACGTGAAGAGGAATGAAGAAGTGCAGCGATTAGGAAGATTCAGCGTGGAGGAGTACAATCGGATGGGAGGCAGCGGCGGAGGAGAGGTGAAATTCGCGGCGGTGATTGCGGCGGAGAGGCAGGTGGTGTCGGGAACGAAGTACTATTTGAGAATTTTGGGGATTCAGAATGGGGAGAGGAAGATTTTTGATTCGGTTGTGATTGTGAAGCCGTGGATTGGTTCTAAGCGCCTTCTGGATTTTTCGGCTTCCAGAGTTTTGAGCACTTCAATTTTTAATTTCTGA

Coding sequence (CDS)

ATGGCGGAAACAAAATTCTCTGTAATAATCGCCGTTGTGGCGGTGGCTTTGGTGGCAGCGGGCGTGGTGGTGGAAGGATACGGCGGAAAAGTAGGCGGAAGAATGGAGATCAAAGACGTGAAGAGGAATGAAGAAGTGCAGCGATTAGGAAGATTCAGCGTGGAGGAGTACAATCGGATGGGAGGCAGCGGCGGAGGAGAGGTGAAATTCGCGGCGGTGATTGCGGCGGAGAGGCAGGTGGTGTCGGGAACGAAGTACTATTTGAGAATTTTGGGGATTCAGAATGGGGAGAGGAAGATTTTTGATTCGGTTGTGATTGTGAAGCCGTGGATTGGTTCTAAGCGCCTTCTGGATTTTTCGGCTTCCAGAGTTTTGAGCACTTCAATTTTTAATTTCTGA

Protein sequence

MAETKFSVIIAVVAVALVAAGVVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRMGGSGGGEVKFAAVIAAERQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFSASRVLSTSIFNF

Homology

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BLAST of Lsi08G003220 vs. ExPASy Swiss-Prot
Match: Q5N806 (Cysteine proteinase inhibitor 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0915200 PE=2 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 1.6e-19
Identity = 61/136 (44.85%), Postives = 83/136 (61.03%), Query Frame = 0


Query: 8   VIIAVVAVALVAAGVVVEGYGG-------------KVGGRMEIKDVKRNEEVQRLGRFSV 67
           ++I +V VA  A+G    G GG             +VGGR E++DV+ + EVQ LGRFSV
Sbjct: 14  LLIVLVTVASAASGARSGGGGGGGIRELRGGGAGRRVGGRTEVRDVEGDREVQELGRFSV 73

Query: 68  EEYNRMGGS---GGGEVKFAAVIAAERQVVSGTKYYLRI-------LGIQNGERKIFDSV 121
           EE+NR   S   G   ++F  V+AA+RQVVSG KYYLR+        G   GE ++FD+V
Sbjct: 74  EEHNRRRRSRDCGDVRLEFGRVVAAQRQVVSGLKYYLRVAAAEEGAAGQNGGEPRVFDAV 133

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BLAST of Lsi08G003220 vs. ExPASy Swiss-Prot
Match: Q0JGM8 (Cysteine proteinase inhibitor 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0915401 PE=3 SV=2)

HSP 1 Score: 94.4 bits (233), Expect = 1.1e-18
Identity = 48/96 (50.00%), Postives = 66/96 (68.75%), Query Frame = 0


Query: 26  GYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRMGGSG--GGEVKFAAVIAAERQVVSG 85
           G G KVGGR +++DV+ N EVQ LG F V E+NR GGS   G  + F+ V+AA+ QVVSG
Sbjct: 44  GRGRKVGGRTDVEDVEGNREVQELGLFCVVEHNRRGGSATRGRGLVFSRVVAAQTQVVSG 103

Query: 86  TKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDF 120
            KYYLRI   +  +  +FD+VV+VK W+ S+ ++ F
Sbjct: 104 IKYYLRIAAQEADDELVFDAVVVVKAWVPSREMVSF 139

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BLAST of Lsi08G003220 vs. ExPASy Swiss-Prot
Match: Q10993 (Cysteine proteinase inhibitor B OS=Helianthus annuus OX=4232 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.1e-18
Identity = 49/97 (50.52%), Postives = 72/97 (74.23%), Query Frame = 0


Query: 32  GGRMEIKDVKRNEEVQRLGRFSVEEYNRM---GGSGGGEVKFAAVIAAERQVVSGTKYYL 91
           GGR ++K+VK + E+Q+LG +SV+EYNR+     +G G++KF+ VIAAE QVV+GTKYYL
Sbjct: 3   GGRTKVKNVKTDTEIQQLGSYSVDEYNRLQRTKKTGAGDLKFSQVIAAETQVVAGTKYYL 62

Query: 92  RILGI-QNGERKIFDSVVIVKPWIGSKRLLDFSASRV 125
           +I  I + G+ K+FD+ V+V+ W  SK+LL F  + V
Sbjct: 63  KIEAITKGGKMKVFDAEVVVQSWKHSKKLLGFKPAPV 99

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BLAST of Lsi08G003220 vs. ExPASy Swiss-Prot
Match: A2XS65 (Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_014908 PE=3 SV=2)

HSP 1 Score: 84.3 bits (207), Expect = 1.1e-15
Identity = 49/107 (45.79%), Postives = 67/107 (62.62%), Query Frame = 0


Query: 31  VGGRMEIKDVKRNEEVQRLGRFSVEEYNRM---GGSGGG------EVKFAAVIAAERQVV 90
           VGGR EI+DV  N+ VQ LGRF+V E+NR    GGSGG       ++ FA V+ A++QVV
Sbjct: 39  VGGRTEIRDVGSNKAVQSLGRFAVAEHNRRLRHGGSGGPADPVPVKLAFARVVEAQKQVV 98

Query: 91  SGTKYYLRIL--------GIQNGERKIFDSVVIVKPWIGSKRLLDFS 121
           SG  YYL++         G   G  ++FD+VV+VK W+ SK L+ F+
Sbjct: 99  SGVAYYLKVAASARDPRGGAAAGGDRVFDAVVVVKAWLKSKELVSFT 145

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BLAST of Lsi08G003220 vs. ExPASy Swiss-Prot
Match: P0C579 (Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0350100 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.1e-15
Identity = 49/107 (45.79%), Postives = 67/107 (62.62%), Query Frame = 0


Query: 31  VGGRMEIKDVKRNEEVQRLGRFSVEEYNRM---GGSGGG------EVKFAAVIAAERQVV 90
           VGGR EI+DV  N+ VQ LGRF+V E+NR    GGSGG       ++ FA V+ A++QVV
Sbjct: 39  VGGRTEIRDVGSNKAVQSLGRFAVAEHNRRLRHGGSGGPADPVPVKLAFARVVEAQKQVV 98

Query: 91  SGTKYYLRIL--------GIQNGERKIFDSVVIVKPWIGSKRLLDFS 121
           SG  YYL++         G   G  ++FD+VV+VK W+ SK L+ F+
Sbjct: 99  SGVAYYLKVAASARDPRGGAAAGGDRVFDAVVVVKAWLKSKELVSFT 145

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BLAST of Lsi08G003220 vs. ExPASy TrEMBL
Match: A0A0A0KC20 (Cystatin OS=Cucumis sativus OX=3659 GN=Csa_6G022340 PE=4 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.1e-47
Identity = 107/132 (81.06%), Postives = 117/132 (88.64%), Query Frame = 0


Query: 1   MAETKFSVIIAVVAVALVAAGVVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRM 60
           MA+TK SV++ V+AVALVA   +VEGYGG+VGGRME+KDV+RNEEVQRLGRFSVEEYNR 
Sbjct: 1   MAKTKLSVMVVVLAVALVA---LVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRR 60

Query: 61  GGSGGGEVKFAAVIAAERQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFS 120
            G GGGEVKF AV+AAERQVVSGTKYYLRILG QNGERK+FDSVVIVKPWIGSKRLLDFS
Sbjct: 61  MG-GGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFS 120

Query: 121 ASRVLSTSIFNF 133
            S V  T IFNF
Sbjct: 121 PSAVFRTPIFNF 128

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BLAST of Lsi08G003220 vs. ExPASy TrEMBL
Match: A0A1S3CPD1 (cysteine proteinase inhibitor B OS=Cucumis melo OX=3656 GN=LOC103503076 PE=4 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 9.5e-47
Identity = 109/132 (82.58%), Postives = 114/132 (86.36%), Query Frame = 0


Query: 1   MAETKFSVIIAVVAVALVAAGVVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRM 60
           MA+TK SV++   AVALV   VVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNR 
Sbjct: 1   MAKTKLSVMLVAAAVALV---VVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRR 60

Query: 61  GGSGGGEVKFAAVIAAERQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFS 120
            G GGGEVKF AV+AA+RQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFS
Sbjct: 61  MG-GGGEVKFTAVVAADRQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFS 120

Query: 121 ASRVLSTSIFNF 133
            S V ST    F
Sbjct: 121 PSTVFSTPFSYF 128

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BLAST of Lsi08G003220 vs. ExPASy TrEMBL
Match: A0A6J1H8E3 (cysteine proteinase inhibitor B OS=Cucurbita moschata OX=3662 GN=LOC111461002 PE=4 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 3.1e-37
Identity = 84/125 (67.20%), Postives = 104/125 (83.20%), Query Frame = 0


Query: 9   IIAVVAVALVAAGVVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRMG-GSGGGE 68
           +IAVV +AL+   V+ EGY G++GGR  IKDV+ NEEVQ LGRFSVEEYNR    SGGGE
Sbjct: 8   LIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRSRRSGGGE 67

Query: 69  VKFAAVIAAERQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFSASRVLST 128
           V+F AV+ AERQVV+GTKYYLRILGI+NG ++IF+SVV+VKPW+GSK+L+DFSASR+  +
Sbjct: 68  VEFLAVLVAERQVVAGTKYYLRILGIENGRKRIFESVVVVKPWLGSKQLIDFSASRLFPS 127

Query: 129 SIFNF 133
            I NF
Sbjct: 128 RISNF 132

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BLAST of Lsi08G003220 vs. ExPASy TrEMBL
Match: A0A6J1KTZ7 (cysteine proteinase inhibitor B-like OS=Cucurbita maxima OX=3661 GN=LOC111498186 PE=4 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.5e-36
Identity = 82/125 (65.60%), Postives = 105/125 (84.00%), Query Frame = 0


Query: 9   IIAVVAVALVAAGVVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRMG-GSGGGE 68
           +IAVV VAL+   ++ EGY G++GGR  IKDV+ N+EVQ LGRFSVEEYNR    SGGG+
Sbjct: 8   LIAVVVVALLEVALLAEGYSGRIGGRRRIKDVRSNKEVQGLGRFSVEEYNRRSRQSGGGK 67

Query: 69  VKFAAVIAAERQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFSASRVLST 128
           V+F AV+AAERQVV+GTKYYLRI+GI+NG ++IF+SVV+VKPW+GSK+L+DFSASR+  +
Sbjct: 68  VEFLAVVAAERQVVAGTKYYLRIVGIENGRKRIFESVVVVKPWLGSKQLIDFSASRLFPS 127

Query: 129 SIFNF 133
            I NF
Sbjct: 128 RISNF 132

[Close]

BLAST of Lsi08G003220 vs. ExPASy TrEMBL
Match: A0A5A7VC11 (Cysteine proteinase inhibitor B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1312G00040 PE=4 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 3.4e-36
Identity = 85/98 (86.73%), Postives = 87/98 (88.78%), Query Frame = 0


Query: 35  MEIKDVKRNEEVQRLGRFSVEEYNRMGGSGGGEVKFAAVIAAERQVVSGTKYYLRILGIQ 94
           MEIKDVKRNEEVQRLGRFSVEEYNR  G GGGEVKF AV+AA+RQVVSGTKYYLRILGIQ
Sbjct: 1   MEIKDVKRNEEVQRLGRFSVEEYNRRMG-GGGEVKFTAVVAADRQVVSGTKYYLRILGIQ 60

Query: 95  NGERKIFDSVVIVKPWIGSKRLLDFSASRVLSTSIFNF 133
           NGERKIFDSVVIVKPWIGSKRLLDFS S V ST    F
Sbjct: 61  NGERKIFDSVVIVKPWIGSKRLLDFSPSTVFSTPFSYF 97

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BLAST of Lsi08G003220 vs. NCBI nr
Match: XP_038886316.1 (cysteine proteinase inhibitor 4 [Benincasa hispida])

HSP 1 Score: 203.8 bits (517), Expect = 9.4e-49
Identity = 112/132 (84.85%), Postives = 116/132 (87.88%), Query Frame = 0


Query: 1   MAETKFSVIIAVVAVALVAAGVVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRM 60
           M +TK SV +A VAVALVAA VV EGYGG+VGGRMEIKDVKRNEEVQRLGRFSVEEYN  
Sbjct: 1   MGKTKLSV-MAAVAVALVAAAVVAEGYGGRVGGRMEIKDVKRNEEVQRLGRFSVEEYNHR 60

Query: 61  GGSGGGEVKFAAVIAAERQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFS 120
            G GGG+VKFAAVIAAERQVVSG KYYLRILGIQNGE KIFDSVVIVKPWIGSKRLLDFS
Sbjct: 61  MGGGGGKVKFAAVIAAERQVVSGIKYYLRILGIQNGESKIFDSVVIVKPWIGSKRLLDFS 120

Query: 121 ASRVLSTSIFNF 133
            S V ST IFNF
Sbjct: 121 PSIVSSTPIFNF 131

[Close]

BLAST of Lsi08G003220 vs. NCBI nr
Match: XP_004138536.1 (cysteine proteinase inhibitor B [Cucumis sativus] >KGN45922.1 hypothetical protein Csa_005586 [Cucumis sativus])

HSP 1 Score: 199.1 bits (505), Expect = 2.3e-47
Identity = 107/132 (81.06%), Postives = 117/132 (88.64%), Query Frame = 0


Query: 1   MAETKFSVIIAVVAVALVAAGVVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRM 60
           MA+TK SV++ V+AVALVA   +VEGYGG+VGGRME+KDV+RNEEVQRLGRFSVEEYNR 
Sbjct: 1   MAKTKLSVMVVVLAVALVA---LVEGYGGRVGGRMEVKDVRRNEEVQRLGRFSVEEYNRR 60

Query: 61  GGSGGGEVKFAAVIAAERQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFS 120
            G GGGEVKF AV+AAERQVVSGTKYYLRILG QNGERK+FDSVVIVKPWIGSKRLLDFS
Sbjct: 61  MG-GGGEVKFTAVVAAERQVVSGTKYYLRILGTQNGERKVFDSVVIVKPWIGSKRLLDFS 120

Query: 121 ASRVLSTSIFNF 133
            S V  T IFNF
Sbjct: 121 PSAVFRTPIFNF 128

[Close]

BLAST of Lsi08G003220 vs. NCBI nr
Match: XP_008465470.1 (PREDICTED: cysteine proteinase inhibitor B [Cucumis melo])

HSP 1 Score: 196.1 bits (497), Expect = 2.0e-46
Identity = 109/132 (82.58%), Postives = 114/132 (86.36%), Query Frame = 0


Query: 1   MAETKFSVIIAVVAVALVAAGVVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRM 60
           MA+TK SV++   AVALV   VVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNR 
Sbjct: 1   MAKTKLSVMLVAAAVALV---VVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRR 60

Query: 61  GGSGGGEVKFAAVIAAERQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFS 120
            G GGGEVKF AV+AA+RQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFS
Sbjct: 61  MG-GGGEVKFTAVVAADRQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFS 120

Query: 121 ASRVLSTSIFNF 133
            S V ST    F
Sbjct: 121 PSTVFSTPFSYF 128

[Close]

BLAST of Lsi08G003220 vs. NCBI nr
Match: XP_022960190.1 (cysteine proteinase inhibitor B [Cucurbita moschata])

HSP 1 Score: 164.5 bits (415), Expect = 6.4e-37
Identity = 84/125 (67.20%), Postives = 104/125 (83.20%), Query Frame = 0


Query: 9   IIAVVAVALVAAGVVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRMG-GSGGGE 68
           +IAVV +AL+   V+ EGY G++GGR  IKDV+ NEEVQ LGRFSVEEYNR    SGGGE
Sbjct: 8   LIAVVVLALLEVAVLAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRSRRSGGGE 67

Query: 69  VKFAAVIAAERQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFSASRVLST 128
           V+F AV+ AERQVV+GTKYYLRILGI+NG ++IF+SVV+VKPW+GSK+L+DFSASR+  +
Sbjct: 68  VEFLAVLVAERQVVAGTKYYLRILGIENGRKRIFESVVVVKPWLGSKQLIDFSASRLFPS 127

Query: 129 SIFNF 133
            I NF
Sbjct: 128 RISNF 132

[Close]

BLAST of Lsi08G003220 vs. NCBI nr
Match: KAG7025292.1 (hypothetical protein SDJN02_11787, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 162.5 bits (410), Expect = 2.4e-36
Identity = 83/125 (66.40%), Postives = 103/125 (82.40%), Query Frame = 0


Query: 9   IIAVVAVALVAAGVVVEGYGGKVGGRMEIKDVKRNEEVQRLGRFSVEEYNRMG-GSGGGE 68
           +IAVV +AL+   ++ EGY G++GGR  IKDV+ NEEVQ LGRFSVEEYNR    SGGGE
Sbjct: 8   LIAVVVLALLEVAILAEGYSGRIGGRRRIKDVRSNEEVQGLGRFSVEEYNRRSRRSGGGE 67

Query: 69  VKFAAVIAAERQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSKRLLDFSASRVLST 128
           VKF AV+ AERQVV+GTKYYLRILGI+NG ++IF+SVV+VKPW+ SK+L+DFSASR+  +
Sbjct: 68  VKFLAVLVAERQVVAGTKYYLRILGIENGRKRIFESVVVVKPWLRSKQLIDFSASRLFPS 127

Query: 129 SIFNF 133
            I NF
Sbjct: 128 RISNF 132

[Close]

BLAST of Lsi08G003220 vs. TAIR 10
Match: AT2G31980.1 (PHYTOCYSTATIN 2 )

HSP 1 Score: 81.3 bits (199), Expect = 6.6e-16
Identity = 40/105 (38.10%), Postives = 69/105 (65.71%), Query Frame = 0


Query: 31  VGGRMEIKDVKRNEEVQRLGRFSVEEYNRMGGSGGGEV--------------KFAAVIAA 90
           +GG+  + +++ N E+Q+LGR+ VE++N+   +  G +              +F+ V++A
Sbjct: 36  LGGKSGVPNIRTNREIQQLGRYCVEQFNQQAQNEQGNIGSIAKTDTAISNPLQFSRVVSA 95

Query: 91  ERQVVSGTKYYLRILGIQ-NGERKIFDSVVIVKPWIGSKRLLDFS 121
           ++QVV+G KYYLRI   Q NG  ++FDSVV+++PW+ SK+LL F+
Sbjct: 96  QKQVVAGLKYYLRIEVTQPNGSTRMFDSVVVIQPWLHSKQLLGFT 140

[Close]

BLAST of Lsi08G003220 vs. TAIR 10
Match: AT3G12490.1 (cystatin B )

HSP 1 Score: 61.2 bits (147), Expect = 7.1e-10
Identity = 31/89 (34.83%), Postives = 58/89 (65.17%), Query Frame = 0


Query: 31  VGGRMEIKDVKRNEEVQRLGRFSVEEYNRMGGSGGGEVKFAAVIAAERQVVSGTKYYLRI 90
           VGG  ++   + + EV+ L RF+V+E+N+        ++FA V+ A+ QVV+GT ++L +
Sbjct: 4   VGGVGDVPANQNSGEVESLARFAVDEHNK---KENALLEFARVVKAKEQVVAGTLHHLTL 63

Query: 91  LGIQNGERKIFDSVVIVKPWIGSKRLLDF 120
             ++ G++K++++ V VKPW+  K L +F
Sbjct: 64  EILEAGQKKLYEAKVWVKPWLNFKELQEF 89

[Close]

BLAST of Lsi08G003220 vs. TAIR 10
Match: AT3G12490.2 (cystatin B )

HSP 1 Score: 59.3 bits (142), Expect = 2.7e-09
Identity = 36/125 (28.80%), Postives = 72/125 (57.60%), Query Frame = 0


Query: 1   MAETKFSVIIAVVAVALVAAGVVVEGYG------GKVGGRMEIKDVKRNEEVQRLGRFSV 60
           M  ++F + I   +++L  + ++    G        VGG  ++   + + EV+ L RF+V
Sbjct: 1   MMRSRFLLFIVFFSLSLFISSLIASDLGFCNEEMALVGGVGDVPANQNSGEVESLARFAV 60

Query: 61  EEYNRMGGSGGGEVKFAAVIAAERQVVSGTKYYLRILGIQNGERKIFDSVVIVKPWIGSK 120
           +E+N+        ++FA V+ A+ QVV+GT ++L +  ++ G++K++++ V VKPW+  K
Sbjct: 61  DEHNK---KENALLEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFK 120

[Close]

BLAST of Lsi08G003220 vs. TAIR 10
Match: AT2G40880.1 (cystatin A )

HSP 1 Score: 57.8 bits (138), Expect = 7.8e-09
Identity = 28/92 (30.43%), Postives = 55/92 (59.78%), Query Frame = 0


Query: 31  VGGRMEIKDVKRNEEVQRLGRFSVEEYNRMGGSGGGEVKFAAVIAAERQVVSGTKYYLRI 90
           +GG  +++  + + E++ L RF+++E+N+        ++F  ++ A  QVV+GT Y+L +
Sbjct: 35  LGGVHDLRGNQNSGEIESLARFAIQEHNKQQNK---ILEFKKIVKAREQVVAGTMYHLTL 94

Query: 91  LGIQNGERKIFDSVVIVKPWIGSKRLLDFSAS 123
              +  + K F++ V VKPW+  K+L +F  S
Sbjct: 95  EAKEGDQTKNFEAKVWVKPWMNFKQLQEFKES 123

[Close]

BLAST of Lsi08G003220 vs. TAIR 10
Match: AT5G05110.1 (Cystatin/monellin family protein )

HSP 1 Score: 55.8 bits (133), Expect = 3.0e-08
Identity = 33/91 (36.26%), Postives = 55/91 (60.44%), Query Frame = 0


Query: 30  KVGGRMEIK-DVKRNEEVQRLGRFSVEEYNRMGGSGGGEVKFAAVIAAERQVVSGTKYYL 89
           K+GG  + K D    +E+  +  F+V+E+NR   +    ++ A V+ A  QVV+G  Y L
Sbjct: 44  KLGGFSDSKNDWNGGKEIDDIALFAVQEHNRRENA---VLELARVLKATEQVVAGKLYRL 103

Query: 90  RILGIQNGERKIFDSVVIVKPWIGSKRLLDF 120
            +  I+ GE+KI+++ V VKPW+  K+L +F
Sbjct: 104 TLEVIEAGEKKIYEAKVWVKPWMNFKQLQEF 131

The following BLAST results are available for this feature:

BLAST of Lsi08G003220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5N806	1.6e-19	44.85	Cysteine proteinase inhibitor 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01...	[more]
Q0JGM8	1.1e-18	50.00	Cysteine proteinase inhibitor 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01...	[more]
Q10993	1.1e-18	50.52	Cysteine proteinase inhibitor B OS=Helianthus annuus OX=4232 PE=1 SV=1	[more]
A2XS65	1.1e-15	45.79	Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_0...	[more]
P0C579	1.1e-15	45.79	Cysteine proteinase inhibitor 10 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]

BLAST of Lsi08G003220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KC20	1.1e-47	81.06	Cystatin OS=Cucumis sativus OX=3659 GN=Csa_6G022340 PE=4 SV=1	[more]
A0A1S3CPD1	9.5e-47	82.58	cysteine proteinase inhibitor B OS=Cucumis melo OX=3656 GN=LOC103503076 PE=4 SV=...	[more]
A0A6J1H8E3	3.1e-37	67.20	cysteine proteinase inhibitor B OS=Cucurbita moschata OX=3662 GN=LOC111461002 PE...	[more]
A0A6J1KTZ7	1.5e-36	65.60	cysteine proteinase inhibitor B-like OS=Cucurbita maxima OX=3661 GN=LOC111498186...	[more]
A0A5A7VC11	3.4e-36	86.73	Cysteine proteinase inhibitor B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_...	[more]

BLAST of Lsi08G003220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886316.1	9.4e-49	84.85	cysteine proteinase inhibitor 4 [Benincasa hispida]	[more]
XP_004138536.1	2.3e-47	81.06	cysteine proteinase inhibitor B [Cucumis sativus] >KGN45922.1 hypothetical prote...	[more]
XP_008465470.1	2.0e-46	82.58	PREDICTED: cysteine proteinase inhibitor B [Cucumis melo]	[more]
XP_022960190.1	6.4e-37	67.20	cysteine proteinase inhibitor B [Cucurbita moschata]	[more]
KAG7025292.1	2.4e-36	66.40	hypothetical protein SDJN02_11787, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Lsi08G003220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G31980.1	6.6e-16	38.10	PHYTOCYSTATIN 2	[more]
AT3G12490.1	7.1e-10	34.83	cystatin B	[more]
AT3G12490.2	2.7e-09	28.80	cystatin B	[more]
AT2G40880.1	7.8e-09	30.43	cystatin A	[more]
AT5G05110.1	3.0e-08	36.26	Cystatin/monellin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 29..122 e-value: 0.002 score: 27.4
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 37..120 e-value: 5.4E-12 score: 45.9
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 43..119 e-value: 2.33447E-11 score: 54.7876
None	No IPR available	GENE3D	3.10.450.10		coord: 29..123 e-value: 6.6E-25 score: 89.3
None	No IPR available	PANTHER	PTHR47373:SF1	CYSTEINE PROTEINASE INHIBITOR 2	coord: 7..122
None	No IPR available	PANTHER	PTHR47373	CYSTEINE PROTEINASE INHIBITOR 2	coord: 7..122
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 37..120

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G003220.1	Lsi08G003220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0016020	membrane
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity