Lcy06g019250 (gene) Sponge gourd (P93075) v1

BLAST of Lcy06g019250 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 8.0e-118
Identity = 218/406 (53.69%), Postives = 311/406 (76.60%), Query Frame = 0

Query: 2   DEIEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSDS 61
           +EIE+P +FLCPISLEIMKDPV + +GITYDR SIE WLF+   +SCPVTK  I+ D+D 
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDIT-DAD- 67

Query: 62  LTPNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLSCIRRLR 121
           LTPNHTLRRLIQ+WCTL++S+G++R PTP+PP+ K +I KLI  ++SS ++ + C++RLR
Sbjct: 68  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 127

Query: 122 SLASDSEANRRSLQSAGAPEFLASLVA--SSDSAVSHEALSTLHNLRLSDSNLKSLTSN- 181
            + S++  N+R L++AG PEFLA++V+  S + +++ EAL+ L++L  S++ LK+L +N 
Sbjct: 128 QIVSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDEALNLLYHLETSETVLKNLLNNK 187

Query: 182 --GDFFDSLTRVMQQGTYESRAYAVLVLKSMVEVADSVELTFLKSDLFVEIVGVLKDQTT 241
              +   SLT++MQ+G YESR YA L+LK+++EVAD ++   LK ++F E+V +L D+  
Sbjct: 188 KDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDR-I 247

Query: 242 SPQSLKAALKVLIVACPWGRNRVKAVEAGGVGALVEILL-AAAAAERRVCEMALTAVDLL 301
           S ++ KAA+ +L+  CPWGRNR KAVEAG +  ++E+L+  +  +ERR  EMA+  +DLL
Sbjct: 248 SQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLL 307

Query: 302 CGCADGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSIAKYSGSPAVLAEMAQVGI 361
           C CA+GRA  L HG  +AVV KKILRVSQ  S+RAVR+L S+ ++  +PA+L EM Q+G+
Sbjct: 308 CQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQLGV 367

Query: 362 VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSPCIPSKLVSSYP 402
           VAKLCLVLQV  G KTKEKAKE+LK+HARVW++SPC+P  ++ +YP
Sbjct: 368 VAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of Lcy06g019250 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 9.8e-116
Identity = 224/435 (51.49%), Postives = 310/435 (71.26%), Query Frame = 0

Query: 3   EIEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSDSL 62
           EIE+P +FLCPISL+IMKDPV + +GITYDR SIE WLFS   +SCPVTK  I+     L
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVIT--ETDL 63

Query: 63  TPNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLSCIRRLRS 122
           TPNHTLRRLIQ+WCTL++S+GI+R PTPKPP+ K +I KLI  +SSS  + + C++RLR 
Sbjct: 64  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 123

Query: 123 LASDSEANRRSLQSAGAPEFLASLVASS-----------------------------DSA 182
           + S++  N+R L++A  PEFLA++V++S                             DS+
Sbjct: 124 IVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSS 183

Query: 183 VS--HEALSTLHNLRLSDSNLKSLTSN---GDFFDSLTRVMQQGTYESRAYAVLVLKSMV 242
            S   EALS L++L  S++ LKSL +N    +   +LT++MQ+G YESRAYA L+LK ++
Sbjct: 184 RSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLL 243

Query: 243 EVADSVELTFLKSDLFVEIVGVLKDQTTSPQSLKAALKVLIVACPWGRNRVKAVEAGGVG 302
           EVAD +++  L+ +LF E++ +L DQ  S ++ ++A+++L++ CPWGRNR KAVE G + 
Sbjct: 244 EVADPMQIILLERELFGEVIQILHDQ-ISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 303

Query: 303 ALVEILL-AAAAAERRVCEMALTAVDLLCGCADGRAALLGHGGGMAVVSKKILRVSQVGS 362
            ++E+L+    ++ERR  EMA+  +D+LC CA+GRA  L HG  +AVVSKKILRVSQ+ S
Sbjct: 304 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 363

Query: 363 ERAVRILFSIAKYSGSPAVLAEMAQVGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWR 403
           ERAVR+L S+ ++  +P++L EM Q+G+VAKLCLVLQV  G+KTKEKAKE+LK+HARVWR
Sbjct: 364 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHARVWR 423

BLAST of Lcy06g019250 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.6e-65
Identity = 169/444 (38.06%), Postives = 240/444 (54.05%), Query Frame = 0

Query: 2   DEIEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSDS 61
           +EIE+P+YF+CPISLEIMKDPVT  SGITYDR +I  WL  +   SCPVTK P+  DSD 
Sbjct: 6   EEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSD- 65

Query: 62  LTPNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHL---SCIR 121
           LTPNH LRRLIQ WC  + + G+ R  TP+ P  K  +++ I +        L     ++
Sbjct: 66  LTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQ 125

Query: 122 RLRSLASDSEANRRSLQSAGAPEFLASLVASSDSAVS---------HEALSTLHNLRLSD 181
           +L  LA D   NRR +   G  + L   V    S             E+L  LH + +  
Sbjct: 126 KLEVLAMDGN-NRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPS 185

Query: 182 SNLKS-LTSNGDFFDSLTRVMQQGTYESRAYAVLVLKSMVEVADSVELTFLKSDLFVEIV 241
           ++ K+ L  N    +SLT V+ Q  + S+AY +++L+++ E   S  +  L  ++F  I+
Sbjct: 186 NDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIFKGII 245

Query: 242 GVLKD-----QTTSP-----------------------------QSLKAALKVLIVACPW 301
           G LKD       TSP                             Q++ AAL +L+    W
Sbjct: 246 GFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSSW 305

Query: 302 GRNRVKAVEAGGVGALVEILLAAAAAERRVCEMALTAVDLLCGCADGRAALLGHGGGMAV 361
            RNR   V+ G V  L+E+ + +   E+R+ E+ L  +  LC CA+GRA +L H GG+AV
Sbjct: 306 SRNRSLLVDLGAVSELIELEI-SYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAV 365

Query: 362 VSKKILRVSQVGSERAVRILFSIAKYSGSPAVLAEMAQVGIVAKLCLVLQVESGSKTKEK 399
           V+K++LRVS    +RA+ IL +++K+S    V+ EM  VG V KLC VL ++ G   KEK
Sbjct: 366 VTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCGLNLKEK 425

BLAST of Lcy06g019250 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.0e-48
Identity = 138/395 (34.94%), Postives = 223/395 (56.46%), Query Frame = 0

Query: 4   IEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSDSLT 63
           I++P++F CPISLE+M+DPVT+ +G TYDR SIE+W+   N+++CPVT+ P+S    +L 
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS--DFTLI 71

Query: 64  PNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLS------CI 123
           PNHTLRRLIQ WC  + S+G++R PTPK P     +  L+S AS+   +H+S       +
Sbjct: 72  PNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAAL 131

Query: 124 RRLRSLASDSEANRRSLQSAGAPEFLASLVAS--SDSAVSHEALSTLHNLRLSDSN-LKS 183
           RRLR  A DS+ NR  + +  A E L  ++ S  + S +  E+L+ L  L +++ N   S
Sbjct: 132 RRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQFVS 191

Query: 184 LTSNGDFFDSLTRVMQQGTYESRAYAVLVLKSMVEVADSVELTFLKSD---LFVEIVGVL 243
           ++S+    + LTR++   + E+R  A  +++ +     S +L    S+   +F  ++ +L
Sbjct: 192 ISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGVLDLL 251

Query: 244 KDQTTSPQSLKAALKVLIVACPWGRNRVKAVEAGGVGALVEILLAAAAAERRVCEMALTA 303
           ++  +S ++LK  +K L   C     R  A+ AG    L++ L  AA  +R   E AL  
Sbjct: 252 RNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRL--AADFDRCDTERALAT 311

Query: 304 VDLLCGCADGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSIAKYSGSPAVLAEMA 363
           V+LLC   +G AA   H   + ++ K ILRVS   +E A   L ++   +       E A
Sbjct: 312 VELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC--TAEERWREEAA 371

Query: 364 QVGIVAKLCLVLQVESGSKTKEKAKEILKMHARVW 387
             G+V +L L++Q E   + K+KA+++LK+    W
Sbjct: 372 GAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of Lcy06g019250 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.3e-48
Identity = 136/406 (33.50%), Postives = 223/406 (54.93%), Query Frame = 0

Query: 4   IEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSDSLT 63
           I++P++F CPISL++M DPVT+ +G TYDR SI++W+   N ++CPVT++ +S    +L 
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVTRVALS--DFTLI 71

Query: 64  PNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLS------CI 123
           PNHTLRRLIQ WC  + S+G++R PTPK P     +  L+S AS+   +H+S       I
Sbjct: 72  PNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAI 131

Query: 124 RRLRSLASDSEANRRSLQSAGAPEFLASLV------ASSDSAVSHEALSTLHNLRLSDSN 183
           RRLR LA DSE NR  +    A E L  ++       S  S +  E+L+ L  L ++++ 
Sbjct: 132 RRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELVSESLALLVLLHMTETE 191

Query: 184 LKSLTSNGDFFDSLTRVMQQGTYESRAYAVLVLKSMVEVADSVELTFLKS---DLFVEIV 243
            +++ S+      +TR++   + E R  A  +++ ++  A S++L  + S    +F  ++
Sbjct: 192 CEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEGVL 251

Query: 244 GVLKDQTTSPQSLKAALKVLIVACPWGRNRVKAVEAGGVGALVEILLAAAAAERRVCEMA 303
            +LK+  +S ++LK  +K +   C   + R  A+ AG  G L++ L  AA  +R   E  
Sbjct: 252 DLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRL--AADFDRCDTERG 311

Query: 304 LTAVDLLCGCADGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSIAKYSGSPAVLA 363
           L  V+LLC   +G AA   H   + ++ K ILRVS   +E A   L ++   +       
Sbjct: 312 LATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC--TAEERCRD 371

Query: 364 EMAQVGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSPCIPS 395
           E A  G+V +L L++Q +   + K KA+ +LK+    W +   + S
Sbjct: 372 EAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTVHS 410

BLAST of Lcy06g019250 vs. ExPASy TrEMBL
Match: A0A6J1KXX6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111498577 PE=4 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.9e-175
Identity = 332/411 (80.78%), Postives = 367/411 (89.29%), Query Frame = 0

Query: 1   MDEIEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSD 60
           M+EIEVP+YFLCPISL+IMKDPVT+PSGITYDR SIETWLFS  ++SCP+TKLPI  DSD
Sbjct: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60

Query: 61  SLTPNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLSCIRRL 120
           SLTPNHTLRRLIQAWCTLHS HGI+RFPTPKPP+ KPQI KLISS+SS+P S LSCIRRL
Sbjct: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120

Query: 121 RSLASDSEANRRSLQSAGAPEFLASLVASSDS--------AVSHEALSTLHNLRLSDSNL 180
           RSLA++SE+NRR ++SAGAPEFLAS++ +S S           HEALSTLHNLRLSDS L
Sbjct: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL 180

Query: 181 KSLTSNGDFFDSLTRVMQQGTYESRAYAVLVLKSMVEVADSVELTFLKSDLFVEIVGVLK 240
           KSLT N + FDSLT+VM+QGTYESRAYA L+LKS++EVAD +ELTFLKS+LFVEIVG+LK
Sbjct: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGILK 240

Query: 241 DQTTSPQSLKAALKVLIVACPWGRNRVKAVEAGGVGALVEILLAAAAAERRVCEMALTAV 300
           DQ TS QSLKAAL +LI AC WGRN+VKAVEAGGV ALVEILL  +  ERRV EMALTA+
Sbjct: 241 DQ-TSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILL--SGPERRVSEMALTAM 300

Query: 301 DLLCGCADGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSIAKYSGSPAVLAEMAQ 360
           DLLCGCA+GRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFS+AKYSGSPAVL EMAQ
Sbjct: 301 DLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQ 360

Query: 361 VGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSPCIPSKLVSSYPAN 404
           +GIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNS CIPSKL SSYPAN
Sbjct: 361 LGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of Lcy06g019250 vs. ExPASy TrEMBL
Match: A0A6J1GKE4 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111455050 PE=4 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.5e-175
Identity = 331/411 (80.54%), Postives = 367/411 (89.29%), Query Frame = 0

Query: 1   MDEIEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSD 60
           M+EIEVP+YFLCPISL+IMKDPVT+PSGITYDR SIETWLFS+ ++SCP+TKLPI  DSD
Sbjct: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSNKNASCPITKLPIPADSD 60

Query: 61  SLTPNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLSCIRRL 120
           SLTPNHTLRRLIQAWCTLHS HGI+RFPTPKPP+ KPQI KLISS+SS+P S LSCIRRL
Sbjct: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120

Query: 121 RSLASDSEANRRSLQSAGAPEFLASLVASS--------DSAVSHEALSTLHNLRLSDSNL 180
           RSLA++SE+NRR ++SAGAPEFLAS++ +S         S   HEALSTLHNLRLSDS L
Sbjct: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETASISCHEALSTLHNLRLSDSKL 180

Query: 181 KSLTSNGDFFDSLTRVMQQGTYESRAYAVLVLKSMVEVADSVELTFLKSDLFVEIVGVLK 240
           KSLT N + FDSLT+VM+QGTYESRAYA L+ KS++EVAD +ELTFLKS+LFVEIVG+LK
Sbjct: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLFKSLLEVADPMELTFLKSELFVEIVGILK 240

Query: 241 DQTTSPQSLKAALKVLIVACPWGRNRVKAVEAGGVGALVEILLAAAAAERRVCEMALTAV 300
           DQ TS QSLKAAL +LI AC WGRN+VKAVEAGGV ALVEILL  +  ERRV EMALTA+
Sbjct: 241 DQ-TSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILL--SGPERRVSEMALTAM 300

Query: 301 DLLCGCADGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSIAKYSGSPAVLAEMAQ 360
           DLLCGCA+GRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFS+AKYSGSPAVL EMAQ
Sbjct: 301 DLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQ 360

Query: 361 VGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSPCIPSKLVSSYPAN 404
           +GIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNS CIPSKL SSYPAN
Sbjct: 361 LGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of Lcy06g019250 vs. ExPASy TrEMBL
Match: A0A5D3DNW0 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004610 PE=4 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.4e-160
Identity = 299/406 (73.65%), Postives = 357/406 (87.93%), Query Frame = 0

Query: 2   DEIEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPIS-PDSD 61
           ++IEVPHYFLCPISL+IMKDPVTLPSGITYDRHSIETWLFS  +SSCP+TKLP+S  DSD
Sbjct: 3   EQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPITKLPVSLSDSD 62

Query: 62  SLTPNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLSCIRRL 121
            LTPNHTLRRLIQAWCTL+SS+G++RFPTPKPP+ K QIL +IS++++SP S +S IRRL
Sbjct: 63  LLTPNHTLRRLIQAWCTLNSSYGVERFPTPKPPIHKSQILHIISTSNTSPSSLISSIRRL 122

Query: 122 RSLASDSEANRRSLQSAGAPEFLASLVASSDSAVSHEALSTLHNLRLSDSNLKSLTSNGD 181
           RS++++SE NRR ++ AGAPEFL S++  SDS+ SHEALSTLHNLRLSDS  KSL ++ +
Sbjct: 123 RSISAESETNRRCVEFAGAPEFLVSVIVDSDSSASHEALSTLHNLRLSDSTFKSLATHPE 182

Query: 182 FFDSLTRVM--QQGTYE-SRAYAVLVLKSMVEVADSVELTFLKSDLFVEIVGVLKDQTTS 241
             +SLT  M  QQGT+E SR YAVL+LKS+VEVAD ++L+FLK +LFV+IV +LKD+++S
Sbjct: 183 VLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKPELFVQIVEILKDRSSS 242

Query: 242 PQSLKAALKVLIVACPWGRNRVKAVEAGGVGALVEILLAAAAAERRVCEMALTAVDLLCG 301
            Q  KAAL +LI   P GRNR+KAVEAGGVGALVEILL  ++ E+RVCEM LTA+DLLCG
Sbjct: 243 LQIFKAALGILIAVSPLGRNRLKAVEAGGVGALVEILL--SSPEKRVCEMTLTAMDLLCG 302

Query: 302 CADGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSIAKYSGSPAVLAEMAQVGIVA 361
           CADGRAALL HGGGMAVVSKKILRVSQ+GSERAVRIL+S+AK+SGSPAVL EMAQ+GIVA
Sbjct: 303 CADGRAALLAHGGGMAVVSKKILRVSQLGSERAVRILYSVAKFSGSPAVLMEMAQLGIVA 362

Query: 362 KLCLVLQVESGSKTKEKAKEILKMHARVWRNSPCIPSKLVSSYPAN 404
           KLCLV+Q+E+GSKTKEKAKEILKMH+RVW+NSPCIPSKL SSYP N
Sbjct: 363 KLCLVMQIENGSKTKEKAKEILKMHSRVWKNSPCIPSKLASSYPTN 406

BLAST of Lcy06g019250 vs. ExPASy TrEMBL
Match: A0A1S3B6U7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486683 PE=4 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.4e-160
Identity = 299/406 (73.65%), Postives = 357/406 (87.93%), Query Frame = 0

Query: 2   DEIEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPIS-PDSD 61
           ++IEVPHYFLCPISL+IMKDPVTLPSGITYDRHSIETWLFS  +SSCP+TKLP+S  DSD
Sbjct: 3   EQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPITKLPVSLSDSD 62

Query: 62  SLTPNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLSCIRRL 121
            LTPNHTLRRLIQAWCTL+SS+G++RFPTPKPP+ K QIL +IS++++SP S +S IRRL
Sbjct: 63  LLTPNHTLRRLIQAWCTLNSSYGVERFPTPKPPIHKSQILHIISTSNTSPSSLISSIRRL 122

Query: 122 RSLASDSEANRRSLQSAGAPEFLASLVASSDSAVSHEALSTLHNLRLSDSNLKSLTSNGD 181
           RS++++SE NRR ++ AGAPEFL S++  SDS+ SHEALSTLHNLRLSDS  KSL ++ +
Sbjct: 123 RSISAESETNRRCVEFAGAPEFLVSVIVDSDSSASHEALSTLHNLRLSDSTFKSLATHPE 182

Query: 182 FFDSLTRVM--QQGTYE-SRAYAVLVLKSMVEVADSVELTFLKSDLFVEIVGVLKDQTTS 241
             +SLT  M  QQGT+E SR YAVL+LKS+VEVAD ++L+FLK +LFV+IV +LKD+++S
Sbjct: 183 VLESLTDFMKLQQGTHESSRTYAVLILKSIVEVADPIQLSFLKPELFVQIVEILKDRSSS 242

Query: 242 PQSLKAALKVLIVACPWGRNRVKAVEAGGVGALVEILLAAAAAERRVCEMALTAVDLLCG 301
            Q  KAAL +LI   P GRNR+KAVEAGGVGALVEILL  ++ E+RVCEM LTA+DLLCG
Sbjct: 243 LQIFKAALGILIAVSPLGRNRLKAVEAGGVGALVEILL--SSPEKRVCEMTLTAMDLLCG 302

Query: 302 CADGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSIAKYSGSPAVLAEMAQVGIVA 361
           CADGRAALL HGGGMAVVSKKILRVSQ+GSERAVRIL+S+AK+SGSPAVL EMAQ+GIVA
Sbjct: 303 CADGRAALLAHGGGMAVVSKKILRVSQLGSERAVRILYSVAKFSGSPAVLMEMAQLGIVA 362

Query: 362 KLCLVLQVESGSKTKEKAKEILKMHARVWRNSPCIPSKLVSSYPAN 404
           KLCLV+Q+E+GSKTKEKAKEILKMH+RVW+NSPCIPSKL SSYP N
Sbjct: 363 KLCLVMQIENGSKTKEKAKEILKMHSRVWKNSPCIPSKLASSYPTN 406

BLAST of Lcy06g019250 vs. ExPASy TrEMBL
Match: A0A0A0LB33 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G782630 PE=4 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 2.3e-160
Identity = 297/406 (73.15%), Postives = 355/406 (87.44%), Query Frame = 0

Query: 2   DEIEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPIS-PDSD 61
           ++IEVPHYFLCPISL+IMKDPVTLPSGITYDRHSIETWLFS  +SSCPVTKLP+S  DSD
Sbjct: 3   EQIEVPHYFLCPISLQIMKDPVTLPSGITYDRHSIETWLFSGKNSSCPVTKLPVSDSDSD 62

Query: 62  SLTPNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLSCIRRL 121
            LTPNHTLRRLIQAWCTL+SSHG++RFPTPKPP+ K QIL +IS++++SP S +S IRRL
Sbjct: 63  LLTPNHTLRRLIQAWCTLNSSHGVERFPTPKPPIHKSQILHIISTSNTSPSSQISSIRRL 122

Query: 122 RSLASDSEANRRSLQSAGAPEFLASLVASSDSAVSHEALSTLHNLRLSDSNLKSLTSNGD 181
           RS++++SE NRR ++ AGAPEFL S++  SDS+ SHEALSTLHNLRLSDS  KSL +  +
Sbjct: 123 RSISAESETNRRCVEFAGAPEFLVSVIVGSDSSASHEALSTLHNLRLSDSTFKSLATRPE 182

Query: 182 FFDSLTRVM--QQGTYE-SRAYAVLVLKSMVEVADSVELTFLKSDLFVEIVGVLKDQTTS 241
           F +SLT  M  QQGT+E SR YAVL+LKS++EVA+ ++L+FLK +LFV+IV +LKD+++S
Sbjct: 183 FLESLTDFMKLQQGTHESSRTYAVLILKSIIEVAEPIQLSFLKPELFVQIVEILKDRSSS 242

Query: 242 PQSLKAALKVLIVACPWGRNRVKAVEAGGVGALVEILLAAAAAERRVCEMALTAVDLLCG 301
            Q  KAAL +LI   P GRNR+KAVEAGGV ALVEILL  ++ E+RVCEM LTA+D+LCG
Sbjct: 243 QQIFKAALGILIAVSPLGRNRLKAVEAGGVRALVEILL--SSPEKRVCEMTLTAMDILCG 302

Query: 302 CADGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSIAKYSGSPAVLAEMAQVGIVA 361
           CADGRAALL HGGGMAVVSKKILRVSQ+GSERAVRIL+S+AK+SGSPAVL EMAQ+GIVA
Sbjct: 303 CADGRAALLAHGGGMAVVSKKILRVSQLGSERAVRILYSVAKFSGSPAVLMEMAQLGIVA 362

Query: 362 KLCLVLQVESGSKTKEKAKEILKMHARVWRNSPCIPSKLVSSYPAN 404
           KLCLVLQ+E+G KTKEKAKEILKMH+R+W+NSPCIPSKL SSYP N
Sbjct: 363 KLCLVLQIENGGKTKEKAKEILKMHSRLWKNSPCIPSKLASSYPTN 406

BLAST of Lcy06g019250 vs. NCBI nr
Match: XP_023005635.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 625.2 bits (1611), Expect = 4.0e-175
Identity = 332/411 (80.78%), Postives = 367/411 (89.29%), Query Frame = 0

Query: 1   MDEIEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSD 60
           M+EIEVP+YFLCPISL+IMKDPVT+PSGITYDR SIETWLFS  ++SCP+TKLPI  DSD
Sbjct: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60

Query: 61  SLTPNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLSCIRRL 120
           SLTPNHTLRRLIQAWCTLHS HGI+RFPTPKPP+ KPQI KLISS+SS+P S LSCIRRL
Sbjct: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120

Query: 121 RSLASDSEANRRSLQSAGAPEFLASLVASSDS--------AVSHEALSTLHNLRLSDSNL 180
           RSLA++SE+NRR ++SAGAPEFLAS++ +S S           HEALSTLHNLRLSDS L
Sbjct: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETALISCHEALSTLHNLRLSDSKL 180

Query: 181 KSLTSNGDFFDSLTRVMQQGTYESRAYAVLVLKSMVEVADSVELTFLKSDLFVEIVGVLK 240
           KSLT N + FDSLT+VM+QGTYESRAYA L+LKS++EVAD +ELTFLKS+LFVEIVG+LK
Sbjct: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGILK 240

Query: 241 DQTTSPQSLKAALKVLIVACPWGRNRVKAVEAGGVGALVEILLAAAAAERRVCEMALTAV 300
           DQ TS QSLKAAL +LI AC WGRN+VKAVEAGGV ALVEILL  +  ERRV EMALTA+
Sbjct: 241 DQ-TSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILL--SGPERRVSEMALTAM 300

Query: 301 DLLCGCADGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSIAKYSGSPAVLAEMAQ 360
           DLLCGCA+GRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFS+AKYSGSPAVL EMAQ
Sbjct: 301 DLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQ 360

Query: 361 VGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSPCIPSKLVSSYPAN 404
           +GIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNS CIPSKL SSYPAN
Sbjct: 361 LGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of Lcy06g019250 vs. NCBI nr
Match: XP_022952378.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata])

HSP 1 Score: 624.8 bits (1610), Expect = 5.2e-175
Identity = 331/411 (80.54%), Postives = 367/411 (89.29%), Query Frame = 0

Query: 1   MDEIEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSD 60
           M+EIEVP+YFLCPISL+IMKDPVT+PSGITYDR SIETWLFS+ ++SCP+TKLPI  DSD
Sbjct: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSNKNASCPITKLPIPADSD 60

Query: 61  SLTPNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLSCIRRL 120
           SLTPNHTLRRLIQAWCTLHS HGI+RFPTPKPP+ KPQI KLISS+SS+P S LSCIRRL
Sbjct: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120

Query: 121 RSLASDSEANRRSLQSAGAPEFLASLVASS--------DSAVSHEALSTLHNLRLSDSNL 180
           RSLA++SE+NRR ++SAGAPEFLAS++ +S         S   HEALSTLHNLRLSDS L
Sbjct: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETASISCHEALSTLHNLRLSDSKL 180

Query: 181 KSLTSNGDFFDSLTRVMQQGTYESRAYAVLVLKSMVEVADSVELTFLKSDLFVEIVGVLK 240
           KSLT N + FDSLT+VM+QGTYESRAYA L+ KS++EVAD +ELTFLKS+LFVEIVG+LK
Sbjct: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLFKSLLEVADPMELTFLKSELFVEIVGILK 240

Query: 241 DQTTSPQSLKAALKVLIVACPWGRNRVKAVEAGGVGALVEILLAAAAAERRVCEMALTAV 300
           DQ TS QSLKAAL +LI AC WGRN+VKAVEAGGV ALVEILL  +  ERRV EMALTA+
Sbjct: 241 DQ-TSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILL--SGPERRVSEMALTAM 300

Query: 301 DLLCGCADGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSIAKYSGSPAVLAEMAQ 360
           DLLCGCA+GRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFS+AKYSGSPAVL EMAQ
Sbjct: 301 DLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGSPAVLTEMAQ 360

Query: 361 VGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSPCIPSKLVSSYPAN 404
           +GIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNS CIPSKL SSYPAN
Sbjct: 361 LGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of Lcy06g019250 vs. NCBI nr
Match: XP_023540239.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 621.7 bits (1602), Expect = 4.4e-174
Identity = 330/411 (80.29%), Postives = 365/411 (88.81%), Query Frame = 0

Query: 1   MDEIEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSD 60
           M+EIEVP+YFLCPISL+IMKDPVT+PSGITYDR SIETWLFS  ++SCP+TKLPI  DSD
Sbjct: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60

Query: 61  SLTPNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLSCIRRL 120
           SLTPNHTLRRLIQAWCTLHS HGI+RFPTPKPP+ KPQI KLISS+SS+P S LSCIRRL
Sbjct: 61  SLTPNHTLRRLIQAWCTLHSDHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120

Query: 121 RSLASDSEANRRSLQSAGAPEFLASLVASS--------DSAVSHEALSTLHNLRLSDSNL 180
           RSLA++SE+NRR ++SAGAPEFLAS++ +S         S   HEALSTLHNLRLSDS L
Sbjct: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETASISCHEALSTLHNLRLSDSKL 180

Query: 181 KSLTSNGDFFDSLTRVMQQGTYESRAYAVLVLKSMVEVADSVELTFLKSDLFVEIVGVLK 240
           KSLT N + FDSLT+VM+QGTYESRAYA L+LKS++EVAD +ELTFLKS+LFVEIVG+LK
Sbjct: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELTFLKSELFVEIVGILK 240

Query: 241 DQTTSPQSLKAALKVLIVACPWGRNRVKAVEAGGVGALVEILLAAAAAERRVCEMALTAV 300
           DQ TS QSLKAAL +LI AC WGRN+VKAVEAGGV ALVEILL  +  ERRV EM LTA+
Sbjct: 241 DQ-TSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILL--SGPERRVSEMVLTAM 300

Query: 301 DLLCGCADGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSIAKYSGSPAVLAEMAQ 360
           DLLCGCA+GRAALLGHGGGMAVVSKKILRVSQVGSERAVRILF +AKYSGSPAVL EMAQ
Sbjct: 301 DLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFWVAKYSGSPAVLTEMAQ 360

Query: 361 VGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSPCIPSKLVSSYPAN 404
           +GIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNS CIPSKL SSYPAN
Sbjct: 361 LGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of Lcy06g019250 vs. NCBI nr
Match: KAG7028156.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 621.3 bits (1601), Expect = 5.8e-174
Identity = 330/411 (80.29%), Postives = 365/411 (88.81%), Query Frame = 0

Query: 1   MDEIEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSD 60
           M+EIEVP+YFLCPISL+IMKDPVT+PSGITYDR SIETWLFS  ++SCP+TKLPI  DSD
Sbjct: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60

Query: 61  SLTPNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLSCIRRL 120
           SLTPNHTLRRLIQAWCTLHS HGI+RFPTPKPP+ KPQI KLISS+SS+P S LSCIRRL
Sbjct: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120

Query: 121 RSLASDSEANRRSLQSAGAPEFLASLVASSDS--------AVSHEALSTLHNLRLSDSNL 180
           RSLA++SE+NRR ++SAGAPEFLAS++ +S S           HEALSTLHNLRLSDS L
Sbjct: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETAPISCHEALSTLHNLRLSDSKL 180

Query: 181 KSLTSNGDFFDSLTRVMQQGTYESRAYAVLVLKSMVEVADSVELTFLKSDLFVEIVGVLK 240
           KSLT N + FDSLT+VM+QGTYESRAYA L+LKS++EVAD +EL FLKS+LFVEIVG+LK
Sbjct: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELAFLKSELFVEIVGILK 240

Query: 241 DQTTSPQSLKAALKVLIVACPWGRNRVKAVEAGGVGALVEILLAAAAAERRVCEMALTAV 300
           DQ TS QSLKAAL +LI AC WGRN+VKAVEAGGV ALVEILL  +  ERRV EMALTA+
Sbjct: 241 DQ-TSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILL--SGPERRVSEMALTAM 300

Query: 301 DLLCGCADGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSIAKYSGSPAVLAEMAQ 360
           DLLCGCA+GRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFS+AKYSG PAVL EMAQ
Sbjct: 301 DLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGCPAVLTEMAQ 360

Query: 361 VGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSPCIPSKLVSSYPAN 404
           +GIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNS CIPSKL SSYPAN
Sbjct: 361 LGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of Lcy06g019250 vs. NCBI nr
Match: KAG6596619.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 620.2 bits (1598), Expect = 1.3e-173
Identity = 329/411 (80.05%), Postives = 364/411 (88.56%), Query Frame = 0

Query: 1   MDEIEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSD 60
           M+EIEVP+YFLCPISL+IMKDPVT+PSGITYDR SIETWLFS  ++SCP+TKLPI  DSD
Sbjct: 1   MEEIEVPNYFLCPISLQIMKDPVTVPSGITYDRPSIETWLFSTKNASCPITKLPIPADSD 60

Query: 61  SLTPNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLSCIRRL 120
           SLTPNHTLRRLIQAWCTLHS HGI+RFPTPKPP+ KPQI KLISS+SS+P S LSCIRRL
Sbjct: 61  SLTPNHTLRRLIQAWCTLHSHHGIERFPTPKPPINKPQIHKLISSSSSTPSSLLSCIRRL 120

Query: 121 RSLASDSEANRRSLQSAGAPEFLASLVASS--------DSAVSHEALSTLHNLRLSDSNL 180
           RSLA++SE+NRR ++SAGAPEFLAS++ +S         S   HEALSTLHNLRLSDS L
Sbjct: 121 RSLAAESESNRRCIESAGAPEFLASVIINSISDNDNETASISCHEALSTLHNLRLSDSKL 180

Query: 181 KSLTSNGDFFDSLTRVMQQGTYESRAYAVLVLKSMVEVADSVELTFLKSDLFVEIVGVLK 240
           KSLT N + FDSLT+VM+QGTYESRAYA L+LKS++EVAD +EL FLKS+LFVEIVG+LK
Sbjct: 181 KSLTVNAELFDSLTKVMEQGTYESRAYAALLLKSLLEVADPMELAFLKSELFVEIVGILK 240

Query: 241 DQTTSPQSLKAALKVLIVACPWGRNRVKAVEAGGVGALVEILLAAAAAERRVCEMALTAV 300
           DQ TS QSLKAAL +LI AC WGRN+VKAVEAGGV ALVEILL  +  ERRV EMALTA+
Sbjct: 241 DQ-TSQQSLKAALGILITACTWGRNKVKAVEAGGVVALVEILL--SGPERRVSEMALTAM 300

Query: 301 DLLCGCADGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSIAKYSGSPAVLAEMAQ 360
           DLLCGCA+GRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFS+AKYSG PAVL EMAQ
Sbjct: 301 DLLCGCAEGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSVAKYSGCPAVLTEMAQ 360

Query: 361 VGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSPCIPSKLVSSYPAN 404
           +GIV KLCLVLQVESGSKTKEKAKEILKMHARVWRNS CIPSKL SSYPAN
Sbjct: 361 LGIVTKLCLVLQVESGSKTKEKAKEILKMHARVWRNSACIPSKLASSYPAN 408

BLAST of Lcy06g019250 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 425.2 bits (1092), Expect = 5.7e-119
Identity = 218/406 (53.69%), Postives = 311/406 (76.60%), Query Frame = 0

Query: 2   DEIEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSDS 61
           +EIE+P +FLCPISLEIMKDPV + +GITYDR SIE WLF+   +SCPVTK  I+ D+D 
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDIT-DAD- 67

Query: 62  LTPNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLSCIRRLR 121
           LTPNHTLRRLIQ+WCTL++S+G++R PTP+PP+ K +I KLI  ++SS ++ + C++RLR
Sbjct: 68  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 127

Query: 122 SLASDSEANRRSLQSAGAPEFLASLVA--SSDSAVSHEALSTLHNLRLSDSNLKSLTSN- 181
            + S++  N+R L++AG PEFLA++V+  S + +++ EAL+ L++L  S++ LK+L +N 
Sbjct: 128 QIVSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDEALNLLYHLETSETVLKNLLNNK 187

Query: 182 --GDFFDSLTRVMQQGTYESRAYAVLVLKSMVEVADSVELTFLKSDLFVEIVGVLKDQTT 241
              +   SLT++MQ+G YESR YA L+LK+++EVAD ++   LK ++F E+V +L D+  
Sbjct: 188 KDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDR-I 247

Query: 242 SPQSLKAALKVLIVACPWGRNRVKAVEAGGVGALVEILL-AAAAAERRVCEMALTAVDLL 301
           S ++ KAA+ +L+  CPWGRNR KAVEAG +  ++E+L+  +  +ERR  EMA+  +DLL
Sbjct: 248 SQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLL 307

Query: 302 CGCADGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSIAKYSGSPAVLAEMAQVGI 361
           C CA+GRA  L HG  +AVV KKILRVSQ  S+RAVR+L S+ ++  +PA+L EM Q+G+
Sbjct: 308 CQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQLGV 367

Query: 362 VAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSPCIPSKLVSSYP 402
           VAKLCLVLQV  G KTKEKAKE+LK+HARVW++SPC+P  ++ +YP
Sbjct: 368 VAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of Lcy06g019250 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 418.3 bits (1074), Expect = 6.9e-117
Identity = 224/435 (51.49%), Postives = 310/435 (71.26%), Query Frame = 0

Query: 3   EIEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSDSL 62
           EIE+P +FLCPISL+IMKDPV + +GITYDR SIE WLFS   +SCPVTK  I+     L
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVIT--ETDL 63

Query: 63  TPNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLSCIRRLRS 122
           TPNHTLRRLIQ+WCTL++S+GI+R PTPKPP+ K +I KLI  +SSS  + + C++RLR 
Sbjct: 64  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 123

Query: 123 LASDSEANRRSLQSAGAPEFLASLVASS-----------------------------DSA 182
           + S++  N+R L++A  PEFLA++V++S                             DS+
Sbjct: 124 IVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSS 183

Query: 183 VS--HEALSTLHNLRLSDSNLKSLTSN---GDFFDSLTRVMQQGTYESRAYAVLVLKSMV 242
            S   EALS L++L  S++ LKSL +N    +   +LT++MQ+G YESRAYA L+LK ++
Sbjct: 184 RSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLL 243

Query: 243 EVADSVELTFLKSDLFVEIVGVLKDQTTSPQSLKAALKVLIVACPWGRNRVKAVEAGGVG 302
           EVAD +++  L+ +LF E++ +L DQ  S ++ ++A+++L++ CPWGRNR KAVE G + 
Sbjct: 244 EVADPMQIILLERELFGEVIQILHDQ-ISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 303

Query: 303 ALVEILL-AAAAAERRVCEMALTAVDLLCGCADGRAALLGHGGGMAVVSKKILRVSQVGS 362
            ++E+L+    ++ERR  EMA+  +D+LC CA+GRA  L HG  +AVVSKKILRVSQ+ S
Sbjct: 304 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 363

Query: 363 ERAVRILFSIAKYSGSPAVLAEMAQVGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWR 403
           ERAVR+L S+ ++  +P++L EM Q+G+VAKLCLVLQV  G+KTKEKAKE+LK+HARVWR
Sbjct: 364 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHARVWR 423

BLAST of Lcy06g019250 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 251.5 bits (641), Expect = 1.1e-66
Identity = 169/444 (38.06%), Postives = 240/444 (54.05%), Query Frame = 0

Query: 2   DEIEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSDS 61
           +EIE+P+YF+CPISLEIMKDPVT  SGITYDR +I  WL  +   SCPVTK P+  DSD 
Sbjct: 20  EEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSD- 79

Query: 62  LTPNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHL---SCIR 121
           LTPNH LRRLIQ WC  + + G+ R  TP+ P  K  +++ I +        L     ++
Sbjct: 80  LTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQ 139

Query: 122 RLRSLASDSEANRRSLQSAGAPEFLASLVASSDSAVS---------HEALSTLHNLRLSD 181
           +L  LA D   NRR +   G  + L   V    S             E+L  LH + +  
Sbjct: 140 KLEVLAMDGN-NRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPS 199

Query: 182 SNLKS-LTSNGDFFDSLTRVMQQGTYESRAYAVLVLKSMVEVADSVELTFLKSDLFVEIV 241
           ++ K+ L  N    +SLT V+ Q  + S+AY +++L+++ E   S  +  L  ++F  I+
Sbjct: 200 NDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIFKGII 259

Query: 242 GVLKD-----QTTSP-----------------------------QSLKAALKVLIVACPW 301
           G LKD       TSP                             Q++ AAL +L+    W
Sbjct: 260 GFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSSW 319

Query: 302 GRNRVKAVEAGGVGALVEILLAAAAAERRVCEMALTAVDLLCGCADGRAALLGHGGGMAV 361
            RNR   V+ G V  L+E+ + +   E+R+ E+ L  +  LC CA+GRA +L H GG+AV
Sbjct: 320 SRNRSLLVDLGAVSELIELEI-SYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAV 379

Query: 362 VSKKILRVSQVGSERAVRILFSIAKYSGSPAVLAEMAQVGIVAKLCLVLQVESGSKTKEK 399
           V+K++LRVS    +RA+ IL +++K+S    V+ EM  VG V KLC VL ++ G   KEK
Sbjct: 380 VTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCGLNLKEK 439

BLAST of Lcy06g019250 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 195.7 bits (496), Expect = 7.3e-50
Identity = 138/395 (34.94%), Postives = 223/395 (56.46%), Query Frame = 0

Query: 4   IEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSDSLT 63
           I++P++F CPISLE+M+DPVT+ +G TYDR SIE+W+   N+++CPVT+ P+S    +L 
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS--DFTLI 71

Query: 64  PNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLS------CI 123
           PNHTLRRLIQ WC  + S+G++R PTPK P     +  L+S AS+   +H+S       +
Sbjct: 72  PNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAAL 131

Query: 124 RRLRSLASDSEANRRSLQSAGAPEFLASLVAS--SDSAVSHEALSTLHNLRLSDSN-LKS 183
           RRLR  A DS+ NR  + +  A E L  ++ S  + S +  E+L+ L  L +++ N   S
Sbjct: 132 RRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQFVS 191

Query: 184 LTSNGDFFDSLTRVMQQGTYESRAYAVLVLKSMVEVADSVELTFLKSD---LFVEIVGVL 243
           ++S+    + LTR++   + E+R  A  +++ +     S +L    S+   +F  ++ +L
Sbjct: 192 ISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGVLDLL 251

Query: 244 KDQTTSPQSLKAALKVLIVACPWGRNRVKAVEAGGVGALVEILLAAAAAERRVCEMALTA 303
           ++  +S ++LK  +K L   C     R  A+ AG    L++ L  AA  +R   E AL  
Sbjct: 252 RNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRL--AADFDRCDTERALAT 311

Query: 304 VDLLCGCADGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSIAKYSGSPAVLAEMA 363
           V+LLC   +G AA   H   + ++ K ILRVS   +E A   L ++   +       E A
Sbjct: 312 VELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC--TAEERWREEAA 371

Query: 364 QVGIVAKLCLVLQVESGSKTKEKAKEILKMHARVW 387
             G+V +L L++Q E   + K+KA+++LK+    W
Sbjct: 372 GAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of Lcy06g019250 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 194.5 bits (493), Expect = 1.6e-49
Identity = 136/406 (33.50%), Postives = 223/406 (54.93%), Query Frame = 0

Query: 4   IEVPHYFLCPISLEIMKDPVTLPSGITYDRHSIETWLFSDNHSSCPVTKLPISPDSDSLT 63
           I++P++F CPISL++M DPVT+ +G TYDR SI++W+   N ++CPVT++ +S    +L 
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVTRVALS--DFTLI 71

Query: 64  PNHTLRRLIQAWCTLHSSHGIQRFPTPKPPVTKPQILKLISSASSSPDSHLS------CI 123
           PNHTLRRLIQ WC  + S+G++R PTPK P     +  L+S AS+   +H+S       I
Sbjct: 72  PNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAI 131

Query: 124 RRLRSLASDSEANRRSLQSAGAPEFLASLV------ASSDSAVSHEALSTLHNLRLSDSN 183
           RRLR LA DSE NR  +    A E L  ++       S  S +  E+L+ L  L ++++ 
Sbjct: 132 RRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELVSESLALLVLLHMTETE 191

Query: 184 LKSLTSNGDFFDSLTRVMQQGTYESRAYAVLVLKSMVEVADSVELTFLKS---DLFVEIV 243
            +++ S+      +TR++   + E R  A  +++ ++  A S++L  + S    +F  ++
Sbjct: 192 CEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEGVL 251

Query: 244 GVLKDQTTSPQSLKAALKVLIVACPWGRNRVKAVEAGGVGALVEILLAAAAAERRVCEMA 303
            +LK+  +S ++LK  +K +   C   + R  A+ AG  G L++ L  AA  +R   E  
Sbjct: 252 DLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRL--AADFDRCDTERG 311

Query: 304 LTAVDLLCGCADGRAALLGHGGGMAVVSKKILRVSQVGSERAVRILFSIAKYSGSPAVLA 363
           L  V+LLC   +G AA   H   + ++ K ILRVS   +E A   L ++   +       
Sbjct: 312 LATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC--TAEERCRD 371

Query: 364 EMAQVGIVAKLCLVLQVESGSKTKEKAKEILKMHARVWRNSPCIPS 395
           E A  G+V +L L++Q +   + K KA+ +LK+    W +   + S
Sbjct: 372 EAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTVHS 410