Tan0021488 (gene) Snake gourd v1

Overview
NameTan0021488
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionpalmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
LocationLG06: 10302954 .. 10305914 (+)
RNA-Seq ExpressionTan0021488
SyntenyTan0021488
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGCATCCAAACAAGAACCAAAGGACATGGTGAAATCTCCTCCAAGGCTCTTTAGGAGGAAAAAATGGACGAAGTTGGACAAAGATGTTGCATTTTCCTTTCTCCTTTTCCATCTACTTTGTATTTTCGCACCGTTTTGTTTCACTTGGGGTCGATTGTTGCTTTCTTTAGTATTAGCCAATTTAACAGGTATGGCAATCAGTGTTTCATATCATAGAAATCTTGCCCATCGAAGCTTCAAACTTCACAAATGGCTTGAGTATTCGCTTGCTTATCTTGCAGCTCATGCACTTCAGGTTCGACATTTTGAAACCTTAATTATGCTCATGGAACAATAAGCTAGGAATAAATGTGATAGTTATAGTTTTATTTGCAAGTTGTACATTCTTGTTTTGTAGCATAGAAATGATTATTTGATAACTGTGTTATGTAATAATTTGCATCACACACTACTAGACATCAAAACATAGAGTTTTATTTTTTCTCTTTTTTGGAGTTCAACAATATGTGCAATGTATGGGTTGAAAATTTAAATCTTTGATTTTTAGTTGACGATATAATATCTTAACCAGTTGAACTATACTCAAGTTATCGTTCAATTATATAAAAAGAAGAGTAGAGTAATGTTTTTGCAGGTTAATAAAGAGCACAACTTGGGTGCAGTTTGTGCAACTCATTTTTCCACTACAAGATTTATCACTTTTTTTTTAAAAAAGAACCAAAATATTTTTTTTATGTGGTTACAGAGTGGGCTGCAAAATATATTGGGTGTAGTGGAGGGTGCACCAAGTATTTTTCATTAATAAAAGCTTTGAAGATGAGGTCTACATGGATAAATATTTTTTTTTTCCTTTTTTATTTTTATTAAACCATGGAATATTAGCTTGCCAAAACTAGTGATAAGCAATAGAGTTGCATTCTCAAACATATATGCTCAACTAATCTTTTGTTTCAAATATCTATTACGTTTGGTAATGGTGGTTTGTTTTCTAAATTTTAGGGGGATCCAATCGATTGGGTGAGTACACACAGATGCCATCATCGATTCGTTGATACAGAAAGAGACCCACATAGTCCTATATATGGATTTTGGTTTAGTCAAATGACATGGTTTTTTGACTCCTATAACTTGACAAGAAAAGTATGTCCAAACTATTTTAATAATTTTCAAAAGATAGACAGAACCAGCTTTGTGTTCTTCTTGAAGCATGGATATCCAGACAATGTCACTGACTTGGAGAGACAAGCCTTCTATAGGTTTATGCATAAAACATATTTTCTTCATCCACTTGCTCTTTCAGCATTGTTGTATGCTATTGGAGGAGTTCCTTTTATCATCTGGGGAATGGTAAAATAAAATTACTCTCATTCTTTTTCCTTTTAATTATTAACCACATTATCAATGATGTATGCTAAGTATTCCTCAAAAAATAAAATGTATATGCTAAGTATATTAATTTTATATAAACCTTTTAAAATTAATTTATATCCCATCTTTATTATGAATTCAACTTTTTTTAGGTCAAAATACCATTTTGGTTCCTGAAAATATATTGGTATGTTGTCTTTTATTGAATGGAAATTATTATTATTCTGCTCCAGAGTCATGACTATGATATATCATTTTTTGTAATACCTTCTATCAAATATTGTACTTTTTTTTTTTCCTGAAACTATCAAATATTGTGCTTACAAAAGTCCTTTATGTTTTTGAAACCATTCATAGTGTGTGAGGTTCGTACTGAGCTTGCATATAACATCTACGGTAAATTCAGTATGTCATATATGGGGAAAGCAACCATGGAATACTGGCGATTTGTCTAGAAACAATTGGTAAGATACTTCACCCCTTTTTTATTCTCTAGCCTTTGAAAGTGAAAGATGATTTCAATGGATTTAAATTTTTATTTTAACACTTAAATTTTCCTACTTATATTGGATTCTCTATAGATTTTTCAAGTGTTTTATTTTAGTCATTAAAATTAATATATATTTTTTTATTATTTTTTAACATGACCGAAATTCGTATATGGAAACCTCGCCCAAAATATAGGAGGTAACTTGTAGACATTTAGTAGACAATCTGAAAATAGAATTTTGGAAACAATAGATTCAATGAAAACAGAGTTGTACTTTATGTTTTCATATATATGTTTGGTAGCAAATTTATAAATTAGACCAATTTAAAACAATTACTCGAAATGTATTCTATAATATATTTATACACCATACTACTACTTGGAGTTACAAAACTAAAATGTTTATTTTTATAAATTTTATATAACTATTATTTTATAATATCATATAACTTATAATATATGATATTATACATATTTTAATTTAACAAAAATAAATTGAGTTTATAACTTGAAATACTGTATTTATTAAAAATTTTATCTTTTTAATATAATGTTGCATTTTAAAATTTTTAATTCAAAATCTAAATATAATGAAAGCATAAAAATGTTGTTTTCAAAATTTTCACTATTTGGATCGACACATAATCAAGAATAGATTTTGAAAACACATCTATCAAATGCATAATCACTTTAAATCTGAAAACATAAAACAAAATTCAGATTGCTGACCAAACGTACCTATTTATCGAAAAAAATGAATAAAAAGATTTTATTAAATAAATTATTAAAACATTAATAACAATTAGAAAAGTGATTTTTATATATAAAAAAAAATTAAATATATAGTTATTATTTTTCTTCAGGTTGGTGGCTTTATTTACTTATGGAGAAGGATGGCACAATAACCACCATGCTTTTGAGTATTCAGCAAGGCATGGTCATAAATGGTGGCAAATTGACGTTGGTTGGTATGTTATTAAGTTTCTTCAAGTCATTGGATTGGCCACTAATGTGAAATTACCTTCTGAAAAGTACAAGCAAAAGTTGGCGGCTTTGGACAAATCAAATCGCACCCTTCTTAAGCCTATGATTAATTAA

mRNA sequence

ATGGAAGCATCCAAACAAGAACCAAAGGACATGGTGAAATCTCCTCCAAGGCTCTTTAGGAGGAAAAAATGGACGAAGTTGGACAAAGATGTTGCATTTTCCTTTCTCCTTTTCCATCTACTTTGTATTTTCGCACCGTTTTGTTTCACTTGGGGTCGATTGTTGCTTTCTTTAGTATTAGCCAATTTAACAGGTATGGCAATCAGTGTTTCATATCATAGAAATCTTGCCCATCGAAGCTTCAAACTTCACAAATGGCTTGAGTATTCGCTTGCTTATCTTGCAGCTCATGCACTTCAGGGGGATCCAATCGATTGGGTGAGTACACACAGATGCCATCATCGATTCGTTGATACAGAAAGAGACCCACATAGTCCTATATATGGATTTTGGTTTAGTCAAATGACATGGTTTTTTGACTCCTATAACTTGACAAGAAAAGTATGTCCAAACTATTTTAATAATTTTCAAAAGATAGACAGAACCAGCTTTGTGTTCTTCTTGAAGCATGGATATCCAGACAATGTCACTGACTTGGAGAGACAAGCCTTCTATAGGTTTATGCATAAAACATATTTTCTTCATCCACTTGCTCTTTCAGCATTGTTGTATGCTATTGGAGGAGTTCCTTTTATCATCTGGGGAATGTGTGTGAGGTTCGTACTGAGCTTGCATATAACATCTACGGTAAATTCAGTATGTCATATATGGGGAAAGCAACCATGGAATACTGGCGATTTGTCTAGAAACAATTGGTTGGTGGCTTTATTTACTTATGGAGAAGGATGGCACAATAACCACCATGCTTTTGAGTATTCAGCAAGGCATGGTCATAAATGGTGGCAAATTGACGTTGGTTGGTATGTTATTAAGTTTCTTCAAGTCATTGGATTGGCCACTAATGTGAAATTACCTTCTGAAAAGTACAAGCAAAAGTTGGCGGCTTTGGACAAATCAAATCGCACCCTTCTTAAGCCTATGATTAATTAA

Coding sequence (CDS)

ATGGAAGCATCCAAACAAGAACCAAAGGACATGGTGAAATCTCCTCCAAGGCTCTTTAGGAGGAAAAAATGGACGAAGTTGGACAAAGATGTTGCATTTTCCTTTCTCCTTTTCCATCTACTTTGTATTTTCGCACCGTTTTGTTTCACTTGGGGTCGATTGTTGCTTTCTTTAGTATTAGCCAATTTAACAGGTATGGCAATCAGTGTTTCATATCATAGAAATCTTGCCCATCGAAGCTTCAAACTTCACAAATGGCTTGAGTATTCGCTTGCTTATCTTGCAGCTCATGCACTTCAGGGGGATCCAATCGATTGGGTGAGTACACACAGATGCCATCATCGATTCGTTGATACAGAAAGAGACCCACATAGTCCTATATATGGATTTTGGTTTAGTCAAATGACATGGTTTTTTGACTCCTATAACTTGACAAGAAAAGTATGTCCAAACTATTTTAATAATTTTCAAAAGATAGACAGAACCAGCTTTGTGTTCTTCTTGAAGCATGGATATCCAGACAATGTCACTGACTTGGAGAGACAAGCCTTCTATAGGTTTATGCATAAAACATATTTTCTTCATCCACTTGCTCTTTCAGCATTGTTGTATGCTATTGGAGGAGTTCCTTTTATCATCTGGGGAATGTGTGTGAGGTTCGTACTGAGCTTGCATATAACATCTACGGTAAATTCAGTATGTCATATATGGGGAAAGCAACCATGGAATACTGGCGATTTGTCTAGAAACAATTGGTTGGTGGCTTTATTTACTTATGGAGAAGGATGGCACAATAACCACCATGCTTTTGAGTATTCAGCAAGGCATGGTCATAAATGGTGGCAAATTGACGTTGGTTGGTATGTTATTAAGTTTCTTCAAGTCATTGGATTGGCCACTAATGTGAAATTACCTTCTGAAAAGTACAAGCAAAAGTTGGCGGCTTTGGACAAATCAAATCGCACCCTTCTTAAGCCTATGATTAATTAA

Protein sequence

MEASKQEPKDMVKSPPRLFRRKKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLVLANLTGMAISVSYHRNLAHRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDLERQAFYRFMHKTYFLHPLALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGKQPWNTGDLSRNNWLVALFTYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLATNVKLPSEKYKQKLAALDKSNRTLLKPMIN
Homology
BLAST of Tan0021488 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 354.0 bits (907), Expect = 1.8e-96
Identity = 165/294 (56.12%), Postives = 206/294 (70.07%), Query Frame = 0

Query: 22  KKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLVLANLTG-MAISVSYHRNLAHRS 81
           ++W  +D       L  HLL + APF F W  + ++  L  +TG + I++S+HRNL+H++
Sbjct: 96  REWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKA 155

Query: 82  FKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIYGFWFSQMTWFFD 141
           FKL KWLEY  AY  A ALQG+PIDWVSTHR HH+F D++RDPHSP+ GFWFS M W FD
Sbjct: 156 FKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFD 215

Query: 142 SYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDLERQAFYRFMHKTYFLHPLALS 201
           +  +T++                       G P+NV DLE+Q FYRF+  TY LHPLAL+
Sbjct: 216 TNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTTYILHPLALA 275

Query: 202 ALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGKQPWNTGDLSRNNWLVALFTYG 261
             LYA+GG PFI+WGM VR V   HIT  VNS CH+WGKQ WNTGDLS+NNW VA   +G
Sbjct: 276 VALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFG 335

Query: 262 EGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLATNVKLPSEKYKQKLA 315
           EGWHNNHHAFE+SARHG +WWQ+D+ WYV+KFLQ IGLAT+VKLPSE  KQ++A
Sbjct: 336 EGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMA 367

BLAST of Tan0021488 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 2.0e-87
Identity = 147/298 (49.33%), Postives = 196/298 (65.77%), Query Frame = 0

Query: 17  RLFRRKKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLVLANLTGMAISVSYHRNL 76
           R    +KW +LD   AF+ L  H LC+ APF FTW  L ++L++  + G+ I+VSYHRNL
Sbjct: 27  RAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITVSYHRNL 86

Query: 77  AHRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIYGFWFSQMT 136
           AHRSFK+ KWLEY  AY    A+QGDPIDWVSTHR HH+F D++RDPHSP  GFWFS + 
Sbjct: 87  AHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLL 146

Query: 137 WFFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDLERQAFYRFMHKTYFLHP 196
           W FD+  L  K                       G   NV DL+RQ +Y+F+ +T   H 
Sbjct: 147 WLFDTGYLVEKC----------------------GRRTNVEDLKRQWYYKFLQRTVLYHI 206

Query: 197 LALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGKQPWNTGDLSRNNWLVAL 256
           L    LLY  GG+ F+ WGM +   +  H+T  +NS+CH+WG + W T D SRN W +++
Sbjct: 207 LTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSV 266

Query: 257 FTYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLATNVKLPSEKYKQKLA 315
           F++GE WHNNHHAFE SAR G +WWQID+ WY+++FL++IGLAT+VKLPSE  ++++A
Sbjct: 267 FSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRMA 302

BLAST of Tan0021488 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.9e-85
Identity = 148/294 (50.34%), Postives = 191/294 (64.97%), Query Frame = 0

Query: 22  KKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLVLANLTGM-AISVSYHRNLAHRS 81
           +KW   D          H+LC+FAP  F+W    +   L  + G+   ++S+HRNL HRS
Sbjct: 38  RKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRS 97

Query: 82  FKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIYGFWFSQMTWFFD 141
           FKL K+LEY  AY+  HALQGDP+ WVSTHR HH++ DT  DPHSPI GFWF  + W FD
Sbjct: 98  FKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFD 157

Query: 142 SYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDLERQAFYRFMHKTYFLHPLALS 201
           S  +  K C  Y                     +N  DL +Q++YRF+ +T+  H    +
Sbjct: 158 SKYIIEK-CGRY---------------------ENAGDLMKQSYYRFLERTFVYHVYLQA 217

Query: 202 ALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGKQPWNTGDLSRNNWLVALFTYG 261
           ALLY  GG PFI+WGM VR +L  H++  VNSVCH WG +PWNTGDLS NNW +A+ T G
Sbjct: 218 ALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWFIAMLTSG 277

Query: 262 EGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLATNVKLPSEKYKQKLA 315
           EGWHNNHHAFEYSARHG +WWQID  WY+IK L+ +GLAT++K+PSE +K+K++
Sbjct: 278 EGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVPSEIHKRKMS 309

BLAST of Tan0021488 vs. ExPASy Swiss-Prot
Match: Q9SID2 (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=2)

HSP 1 Score: 316.6 bits (810), Expect = 3.3e-85
Identity = 146/297 (49.16%), Postives = 194/297 (65.32%), Query Frame = 0

Query: 18  LFRRKKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLVLANLTGMAISVSYHRNLA 77
           +F  ++W +LD     +    H L + APF FTW  L ++ +   + G+ I+VSYHRNLA
Sbjct: 30  VFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLA 89

Query: 78  HRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIYGFWFSQMTW 137
           HRSFK+ KWLEY LAY A  A+QGDPIDWVSTHR HH+F D+ERDPHSP  GFWFS + W
Sbjct: 90  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLW 149

Query: 138 FFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDLERQAFYRFMHKTYFLHPL 197
            +DS  L  K                       G   NV DL+RQ FYRF+ KT   H L
Sbjct: 150 IYDSAYLVSKC----------------------GRRANVEDLKRQWFYRFLQKTVLFHIL 209

Query: 198 ALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGKQPWNTGDLSRNNWLVALF 257
            L   L+ +GG+ F+ WGM V   L +H+T  +NS+CHIWG + W T D SRN W +++F
Sbjct: 210 GLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVF 269

Query: 258 TYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLATNVKLPSEKYKQKLA 315
           ++GE WHNNHHAFE SAR G +WWQID+ WY+++F ++IGLAT+VK+P+E  ++++A
Sbjct: 270 SFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQRRRMA 304

BLAST of Tan0021488 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 5.6e-85
Identity = 150/277 (54.15%), Postives = 187/277 (67.51%), Query Frame = 0

Query: 39  HLLCIFAPFCFTWGRLLLSLVLANLTGM-AISVSYHRNLAHRSFKLHKWLEYSLAYLAAH 98
           H+LC+ APF F+W    LSL L  + G+   ++SYHRNL HRSFKL K+LEY  AY+  H
Sbjct: 52  HVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSYHRNLTHRSFKLPKYLEYFFAYVGLH 111

Query: 99  ALQGDPIDWVSTHRCHHRFVDTERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQ 158
           ALQGDP+ WVSTHR HH+F DT  DPHSPI GFWFS + W FDS  +  + C  Y     
Sbjct: 112 ALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGFWFSHIFWLFDSKYILEE-CGRY----- 171

Query: 159 KIDRTSFVFFLKHGYPDNVTDLERQAFYRFMHKTYFLHPLALSALLYAIGGVPFIIWGMC 218
                           +N  DL +Q++YRF+ +T+  H    +ALLY  GG PFI+WGM 
Sbjct: 172 ----------------ENAGDLLKQSYYRFLERTFVFHVYLQAALLYMFGGFPFIVWGMA 231

Query: 219 VRFVLSLHITSTVNSVCHIWGKQPWNTGDLSRNNWLVALFTYGEGWHNNHHAFEYSARHG 278
           VR V   H +  VNSVCH +G Q W+TGDLS NNW +A+ T GEGWHNNHHAFEYSARHG
Sbjct: 232 VRTVWGFHASWLVNSVCHRYGHQAWDTGDLSTNNWFIAMLTSGEGWHNNHHAFEYSARHG 291

Query: 279 HKWWQIDVGWYVIKFLQVIGLATNVKLPSEKYKQKLA 315
            +WWQID  WYVIK L+ +GLAT+VK+PSE +K+K++
Sbjct: 292 IEWWQIDTTWYVIKLLEYLGLATDVKVPSEVHKRKMS 306

BLAST of Tan0021488 vs. NCBI nr
Match: XP_038899717.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 588.2 bits (1515), Expect = 4.4e-164
Identity = 271/329 (82.37%), Postives = 302/329 (91.79%), Query Frame = 0

Query: 2   EASKQEPKDMV-KSPPRLFRRKKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLVL 61
           EASK+ PK+MV KSPPRLFRRKKWTK D++VAF FL+ HLLCI APF F+W  L L+L L
Sbjct: 3   EASKEGPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLILHLLCILAPFYFSWTCLFLALGL 62

Query: 62  ANLTGMAISVSYHRNLAHRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDTE 121
           + LTGM ISVSYHRNLAHRSFKL KWLEYSLAY+A HA+QGDPIDWVSTHRCHHR+VD+E
Sbjct: 63  SYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRYVDSE 122

Query: 122 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDLE 181
           RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQK++R +F+F++KHG+PDNVTDLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGHPDNVTDLE 182

Query: 182 RQAFYRFMHKTYFLHPLALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGKQ 241
           +Q FYRF+HKTY+LHPLALS LLYAIGGVPFIIWGMCVRFV SLH+TS VNSVCHIWGKQ
Sbjct: 183 KQRFYRFIHKTYWLHPLALSVLLYAIGGVPFIIWGMCVRFVFSLHVTSMVNSVCHIWGKQ 242

Query: 242 PWNTGDLSRNNWLVALFTYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLAT 301
           PWNTGDLS+NNW+VA+ T+GEGWHNNHHAFEYSARHGH+WWQID GWY+IKFLQVIGLAT
Sbjct: 243 PWNTGDLSKNNWMVAILTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLQVIGLAT 302

Query: 302 NVKLPSEKYKQKLAALDKSNRTLLKPMIN 330
           NVKLPSE  KQKLAAL+KS  T LKPMI+
Sbjct: 303 NVKLPSE--KQKLAALNKSKPTFLKPMID 329

BLAST of Tan0021488 vs. NCBI nr
Match: XP_008451258.1 (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 584.7 bits (1506), Expect = 4.9e-163
Identity = 271/329 (82.37%), Postives = 299/329 (90.88%), Query Frame = 0

Query: 2   EASKQEPKDMV-KSPPRLFRRKKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLVL 61
           EASK+E K+MV  SP RLF+RKKWTK DK+VAF FLL HLLCI APF F+W  L LSL L
Sbjct: 3   EASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSLGL 62

Query: 62  ANLTGMAISVSYHRNLAHRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDTE 121
           ANLTGM ISVSYHRNLAHRSFKL KWLEYSLAY+AAHA+QGDPIDWVSTHRCHHRFVD+E
Sbjct: 63  ANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 122

Query: 122 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDLE 181
           RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQK++R +F+F++KHGY DNVTDLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTDLE 182

Query: 182 RQAFYRFMHKTYFLHPLALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGKQ 241
           +Q FYRF+HKTY+LHPLALS LLYAIGG+PFIIWGMCVRFV+SLH TS +NSVCHIWG Q
Sbjct: 183 KQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWGNQ 242

Query: 242 PWNTGDLSRNNWLVALFTYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLAT 301
           PWNTGDLS+NNW+VA+FT+GEGWHNNHHAFEYSARHGH+WWQID GWY+IKFLQVIG AT
Sbjct: 243 PWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGFAT 302

Query: 302 NVKLPSEKYKQKLAALDKSNRTLLKPMIN 330
           NVKLPSE  KQK AAL+KS  T LKPMI+
Sbjct: 303 NVKLPSE--KQKWAALNKSKPTFLKPMID 329

BLAST of Tan0021488 vs. NCBI nr
Match: XP_011659770.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus])

HSP 1 Score: 583.2 bits (1502), Expect = 1.4e-162
Identity = 271/329 (82.37%), Postives = 303/329 (92.10%), Query Frame = 0

Query: 3   ASKQEPKDMV-KSPPRLFRRKKWTKLDKDVAFSFLLFHLLCIFAP-FCFTWGRLLLSLVL 62
           ASK++PK+MV  SP RLF+RKKWTK DK+VAF FLL HLL I AP F F+WG LLLSL L
Sbjct: 4   ASKEDPKEMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLSLGL 63

Query: 63  ANLTGMAISVSYHRNLAHRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDTE 122
           ANLTGMAISVSYHRNLAHRSFKL KWLEYSLAY+AAHA+QGDPIDWVSTHRCHHRFVD+E
Sbjct: 64  ANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 123

Query: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDLE 182
           RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNN+QK++R  F+F++KHGYPDNVTDLE
Sbjct: 124 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLE 183

Query: 183 RQAFYRFMHKTYFLHPLALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGKQ 242
           +Q FYRF+HKTY+LHPLALS LLYA+GG+PFIIWG+CVRFVLSLH TS +NSVCHIWG Q
Sbjct: 184 KQKFYRFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQ 243

Query: 243 PWNTGDLSRNNWLVALFTYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLAT 302
           PWNTGDLS+NNW+VA+FT+GEGWHNNHHAFEYSARHGH+WWQID GWY+IKFLQVIGLAT
Sbjct: 244 PWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLAT 303

Query: 303 NVKLPSEKYKQKLAALDKSNRTLLKPMIN 330
           NVKLPSE  KQK+AAL+KS  T L+PMI+
Sbjct: 304 NVKLPSE--KQKMAALNKSKPTFLRPMID 330

BLAST of Tan0021488 vs. NCBI nr
Match: XP_011659261.2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus] >KAE8646293.1 hypothetical protein Csa_016268 [Cucumis sativus])

HSP 1 Score: 572.4 bits (1474), Expect = 2.5e-159
Identity = 266/329 (80.85%), Postives = 296/329 (89.97%), Query Frame = 0

Query: 2   EASKQEPKDMV-KSPPRLFRRKKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLVL 61
           E SK++PK+MV KSPPRLFRRKKWTK D++VAF FL  HLLCI APF F+W  LLL+L L
Sbjct: 3   EVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGL 62

Query: 62  ANLTGMAISVSYHRNLAHRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDTE 121
           + LTGM ISVSYHRNLAHRSFKL KWLEYSLAY+A HA+QGDPIDWVSTHRCHHRFVD+ 
Sbjct: 63  SYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSA 122

Query: 122 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDLE 181
           RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNN+QK++R   +F++KHGYPDNVTDLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLE 182

Query: 182 RQAFYRFMHKTYFLHPLALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGKQ 241
           +Q FYRF+HKTY+LHPLALS LLYAIGG+PFIIWGMCVRFV SLH+TS VNSVCHIWG Q
Sbjct: 183 KQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQ 242

Query: 242 PWNTGDLSRNNWLVALFTYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLAT 301
           PWNTGDLS+NNW+VAL T+GEGWHNNHHAFEYSARHGH+WWQID GWY+I+FLQVIGLAT
Sbjct: 243 PWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLAT 302

Query: 302 NVKLPSEKYKQKLAALDKSNRTLLKPMIN 330
           NVKLPSE  KQKLAAL+KS    LK MI+
Sbjct: 303 NVKLPSE--KQKLAALNKSKPIFLKLMID 329

BLAST of Tan0021488 vs. NCBI nr
Match: XP_022959667.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 567.8 bits (1462), Expect = 6.2e-158
Identity = 261/316 (82.59%), Postives = 286/316 (90.51%), Query Frame = 0

Query: 1   MEASKQEPKDMV-KSPPRLFRRKKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLV 60
           MEA+KQEPKDMV  SPP LFRRKKWTKLDK++A  FL+ HLLC+FAPFCFTWGR  L+++
Sbjct: 1   MEATKQEPKDMVINSPPSLFRRKKWTKLDKNLALVFLILHLLCVFAPFCFTWGRFFLAIL 60

Query: 61  LANLTGMAISVSYHRNLAHRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDT 120
           LANLTG+ ISVSYHRNLAHRSFKL KWLEYSLAY  AHALQGDPIDWVSTHRCHHR+VD+
Sbjct: 61  LANLTGIGISVSYHRNLAHRSFKLPKWLEYSLAYCGAHALQGDPIDWVSTHRCHHRYVDS 120

Query: 121 ERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDL 180
           ERDPHSPIYGFWFSQ+ W FDSYNLT+KVCPNY N  QKI+R  F+ F+KHGYPDNV DL
Sbjct: 121 ERDPHSPIYGFWFSQIMWLFDSYNLTKKVCPNY-NTSQKIERGKFMSFMKHGYPDNVNDL 180

Query: 181 ERQAFYRFMHKTYFLHPLALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGK 240
             Q+FY+FMH+TYFLHPLAL ALLYAIGG PFIIWGM VRFVLSLHITS VNSVCHIWGK
Sbjct: 181 LNQSFYKFMHRTYFLHPLALMALLYAIGGAPFIIWGMSVRFVLSLHITSMVNSVCHIWGK 240

Query: 241 QPWNTGDLSRNNWLVALFTYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLA 300
           QPWNTGD+SRNNWLVALFT+GEGWHNNHHAFEYSARHGH+WWQID GWY+IK LQVIGLA
Sbjct: 241 QPWNTGDMSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKLLQVIGLA 300

Query: 301 TNVKLPSEKYKQKLAA 316
           T+VKLPS+ YKQKLAA
Sbjct: 301 THVKLPSQNYKQKLAA 315

BLAST of Tan0021488 vs. ExPASy TrEMBL
Match: A0A1S3BS49 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492608 PE=3 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.4e-163
Identity = 271/329 (82.37%), Postives = 299/329 (90.88%), Query Frame = 0

Query: 2   EASKQEPKDMV-KSPPRLFRRKKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLVL 61
           EASK+E K+MV  SP RLF+RKKWTK DK+VAF FLL HLLCI APF F+W  L LSL L
Sbjct: 3   EASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSLGL 62

Query: 62  ANLTGMAISVSYHRNLAHRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDTE 121
           ANLTGM ISVSYHRNLAHRSFKL KWLEYSLAY+AAHA+QGDPIDWVSTHRCHHRFVD+E
Sbjct: 63  ANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 122

Query: 122 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDLE 181
           RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQK++R +F+F++KHGY DNVTDLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTDLE 182

Query: 182 RQAFYRFMHKTYFLHPLALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGKQ 241
           +Q FYRF+HKTY+LHPLALS LLYAIGG+PFIIWGMCVRFV+SLH TS +NSVCHIWG Q
Sbjct: 183 KQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWGNQ 242

Query: 242 PWNTGDLSRNNWLVALFTYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLAT 301
           PWNTGDLS+NNW+VA+FT+GEGWHNNHHAFEYSARHGH+WWQID GWY+IKFLQVIG AT
Sbjct: 243 PWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGFAT 302

Query: 302 NVKLPSEKYKQKLAALDKSNRTLLKPMIN 330
           NVKLPSE  KQK AAL+KS  T LKPMI+
Sbjct: 303 NVKLPSE--KQKWAALNKSKPTFLKPMID 329

BLAST of Tan0021488 vs. ExPASy TrEMBL
Match: A0A0A0KAB1 (FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G376370 PE=3 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 1.9e-160
Identity = 267/329 (81.16%), Postives = 297/329 (90.27%), Query Frame = 0

Query: 2   EASKQEPKDMV-KSPPRLFRRKKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLVL 61
           E SK++PK+MV KSPPRLFRRKKWTK D++VAF FL  HLLCI APF F+W  LLL+L L
Sbjct: 3   EVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGL 62

Query: 62  ANLTGMAISVSYHRNLAHRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDTE 121
           + LTGM ISVSYHRNLAHRSFKL KWLEYSLAY+A HA+QGDPIDWVSTHRCHHRFVD+ 
Sbjct: 63  SYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSA 122

Query: 122 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDLE 181
           RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNN+QK++R  F+F++KHGYPDNVTDLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLE 182

Query: 182 RQAFYRFMHKTYFLHPLALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGKQ 241
           +Q FYRF+HKTY+LHPLALS LLYAIGG+PFIIWGMCVRFV SLH+TS VNSVCHIWG Q
Sbjct: 183 KQKFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQ 242

Query: 242 PWNTGDLSRNNWLVALFTYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLAT 301
           PWNTGDLS+NNW+VAL T+GEGWHNNHHAFEYSARHGH+WWQID GWY+I+FLQVIGLAT
Sbjct: 243 PWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLAT 302

Query: 302 NVKLPSEKYKQKLAALDKSNRTLLKPMIN 330
           NVKLPSE  KQKLAAL+KS    LK MI+
Sbjct: 303 NVKLPSE--KQKLAALNKSKPIFLKLMID 329

BLAST of Tan0021488 vs. ExPASy TrEMBL
Match: A0A6J1H566 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460678 PE=3 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 3.0e-158
Identity = 261/316 (82.59%), Postives = 286/316 (90.51%), Query Frame = 0

Query: 1   MEASKQEPKDMV-KSPPRLFRRKKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLV 60
           MEA+KQEPKDMV  SPP LFRRKKWTKLDK++A  FL+ HLLC+FAPFCFTWGR  L+++
Sbjct: 1   MEATKQEPKDMVINSPPSLFRRKKWTKLDKNLALVFLILHLLCVFAPFCFTWGRFFLAIL 60

Query: 61  LANLTGMAISVSYHRNLAHRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDT 120
           LANLTG+ ISVSYHRNLAHRSFKL KWLEYSLAY  AHALQGDPIDWVSTHRCHHR+VD+
Sbjct: 61  LANLTGIGISVSYHRNLAHRSFKLPKWLEYSLAYCGAHALQGDPIDWVSTHRCHHRYVDS 120

Query: 121 ERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDL 180
           ERDPHSPIYGFWFSQ+ W FDSYNLT+KVCPNY N  QKI+R  F+ F+KHGYPDNV DL
Sbjct: 121 ERDPHSPIYGFWFSQIMWLFDSYNLTKKVCPNY-NTSQKIERGKFMSFMKHGYPDNVNDL 180

Query: 181 ERQAFYRFMHKTYFLHPLALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGK 240
             Q+FY+FMH+TYFLHPLAL ALLYAIGG PFIIWGM VRFVLSLHITS VNSVCHIWGK
Sbjct: 181 LNQSFYKFMHRTYFLHPLALMALLYAIGGAPFIIWGMSVRFVLSLHITSMVNSVCHIWGK 240

Query: 241 QPWNTGDLSRNNWLVALFTYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLA 300
           QPWNTGD+SRNNWLVALFT+GEGWHNNHHAFEYSARHGH+WWQID GWY+IK LQVIGLA
Sbjct: 241 QPWNTGDMSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKLLQVIGLA 300

Query: 301 TNVKLPSEKYKQKLAA 316
           T+VKLPS+ YKQKLAA
Sbjct: 301 THVKLPSQNYKQKLAA 315

BLAST of Tan0021488 vs. ExPASy TrEMBL
Match: A0A6J1KRZ2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111497720 PE=3 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 3.9e-158
Identity = 258/316 (81.65%), Postives = 287/316 (90.82%), Query Frame = 0

Query: 1   MEASKQEPKDMV-KSPPRLFRRKKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLV 60
           MEASKQEPKDMV  +PP LFRRKKWTKLDK++A  FL+ HLLC+FAPFCFTWGR  L+++
Sbjct: 1   MEASKQEPKDMVINTPPSLFRRKKWTKLDKNLALVFLILHLLCVFAPFCFTWGRFFLTIL 60

Query: 61  LANLTGMAISVSYHRNLAHRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDT 120
           LANLTG+ ISVSYHRNLAHRSFKL KWLEYSLAY  AHALQGDPIDWVSTHRCHHR+ D+
Sbjct: 61  LANLTGIGISVSYHRNLAHRSFKLPKWLEYSLAYCGAHALQGDPIDWVSTHRCHHRYTDS 120

Query: 121 ERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDL 180
           ERDPHSPIYGFWFSQ+TW FDSYNLT+KVCPNY N  QKI+R+ F+ F+KHGYPDNV DL
Sbjct: 121 ERDPHSPIYGFWFSQITWLFDSYNLTKKVCPNY-NTSQKIERSKFMSFMKHGYPDNVNDL 180

Query: 181 ERQAFYRFMHKTYFLHPLALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGK 240
             ++FY+FMH+TYFLHPLAL  LLY+IGG PFIIWGM VRFVLSLHITS VNSVCH+WGK
Sbjct: 181 LNESFYKFMHRTYFLHPLALLVLLYSIGGAPFIIWGMSVRFVLSLHITSMVNSVCHVWGK 240

Query: 241 QPWNTGDLSRNNWLVALFTYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLA 300
           QPWNTGD+SRNNWLVALFT+GEGWHNNHHAFEYSARHGH+WWQID+GWY+IKFLQVIGLA
Sbjct: 241 QPWNTGDMSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDLGWYIIKFLQVIGLA 300

Query: 301 TNVKLPSEKYKQKLAA 316
           T+VKLPSE YKQKL A
Sbjct: 301 THVKLPSENYKQKLPA 315

BLAST of Tan0021488 vs. ExPASy TrEMBL
Match: A0A1S3BQG5 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492609 PE=3 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 6.7e-158
Identity = 260/330 (78.79%), Postives = 294/330 (89.09%), Query Frame = 0

Query: 2   EASKQEPKDMV--KSPPRLFRRKKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLV 61
           E SK++PK+++  KSPP+LFRRKKWTK D++ AF FL FHLLCI APFCF+W RLLL+L 
Sbjct: 4   EVSKEKPKEIILMKSPPKLFRRKKWTKFDRNFAFYFLTFHLLCILAPFCFSWTRLLLALG 63

Query: 62  LANLTGMAISVSYHRNLAHRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDT 121
           L+ L+GM ISVSYHRNLAHRSFKL KW EYSLAY+AAHA+QGDPIDWVSTHRCHHRFVD+
Sbjct: 64  LSCLSGMGISVSYHRNLAHRSFKLPKWFEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 123

Query: 122 ERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDL 181
           ERDPHSPIYGFWFSQMTW FDSYNLTRKVCPNYFNN+QK +R  F+ ++KHGYPDNV DL
Sbjct: 124 ERDPHSPIYGFWFSQMTWLFDSYNLTRKVCPNYFNNYQKAERNIFMLYMKHGYPDNVPDL 183

Query: 182 ERQAFYRFMHKTYFLHPLALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGK 241
           E+Q FYRF+HKTY+LHPLALS LLYAIGG+PFIIWGMCVRFV S+H+TS VNSVCH+WG 
Sbjct: 184 EKQWFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSVHVTSMVNSVCHMWGN 243

Query: 242 QPWNTGDLSRNNWLVALFTYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLA 301
           QPWNTGDLS+NNW+VAL T+GEGWHNNHHAFEYSARHG +WWQID GWY I+FL+VIGLA
Sbjct: 244 QPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGLEWWQIDYGWYFIRFLEVIGLA 303

Query: 302 TNVKLPSEKYKQKLAALDKSNRTLLKPMIN 330
           TNVK PSE  KQKLAAL+KS  T LKP IN
Sbjct: 304 TNVKFPSE--KQKLAALNKSKPTFLKPTIN 331

BLAST of Tan0021488 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 354.0 bits (907), Expect = 1.3e-97
Identity = 165/294 (56.12%), Postives = 206/294 (70.07%), Query Frame = 0

Query: 22  KKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLVLANLTG-MAISVSYHRNLAHRS 81
           ++W  +D       L  HLL + APF F W  + ++  L  +TG + I++S+HRNL+H++
Sbjct: 96  REWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKA 155

Query: 82  FKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIYGFWFSQMTWFFD 141
           FKL KWLEY  AY  A ALQG+PIDWVSTHR HH+F D++RDPHSP+ GFWFS M W FD
Sbjct: 156 FKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFD 215

Query: 142 SYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDLERQAFYRFMHKTYFLHPLALS 201
           +  +T++                       G P+NV DLE+Q FYRF+  TY LHPLAL+
Sbjct: 216 TNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTTYILHPLALA 275

Query: 202 ALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGKQPWNTGDLSRNNWLVALFTYG 261
             LYA+GG PFI+WGM VR V   HIT  VNS CH+WGKQ WNTGDLS+NNW VA   +G
Sbjct: 276 VALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFG 335

Query: 262 EGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLATNVKLPSEKYKQKLA 315
           EGWHNNHHAFE+SARHG +WWQ+D+ WYV+KFLQ IGLAT+VKLPSE  KQ++A
Sbjct: 336 EGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMA 367

BLAST of Tan0021488 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 323.9 bits (829), Expect = 1.5e-88
Identity = 147/298 (49.33%), Postives = 196/298 (65.77%), Query Frame = 0

Query: 17  RLFRRKKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLVLANLTGMAISVSYHRNL 76
           R    +KW +LD   AF+ L  H LC+ APF FTW  L ++L++  + G+ I+VSYHRNL
Sbjct: 27  RAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITVSYHRNL 86

Query: 77  AHRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIYGFWFSQMT 136
           AHRSFK+ KWLEY  AY    A+QGDPIDWVSTHR HH+F D++RDPHSP  GFWFS + 
Sbjct: 87  AHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLL 146

Query: 137 WFFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDLERQAFYRFMHKTYFLHP 196
           W FD+  L  K                       G   NV DL+RQ +Y+F+ +T   H 
Sbjct: 147 WLFDTGYLVEKC----------------------GRRTNVEDLKRQWYYKFLQRTVLYHI 206

Query: 197 LALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGKQPWNTGDLSRNNWLVAL 256
           L    LLY  GG+ F+ WGM +   +  H+T  +NS+CH+WG + W T D SRN W +++
Sbjct: 207 LTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSV 266

Query: 257 FTYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLATNVKLPSEKYKQKLA 315
           F++GE WHNNHHAFE SAR G +WWQID+ WY+++FL++IGLAT+VKLPSE  ++++A
Sbjct: 267 FSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRMA 302

BLAST of Tan0021488 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 316.6 bits (810), Expect = 2.3e-86
Identity = 146/297 (49.16%), Postives = 194/297 (65.32%), Query Frame = 0

Query: 18  LFRRKKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLVLANLTGMAISVSYHRNLA 77
           +F  ++W +LD     +    H L + APF FTW  L ++ +   + G+ I+VSYHRNLA
Sbjct: 30  VFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLA 89

Query: 78  HRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIYGFWFSQMTW 137
           HRSFK+ KWLEY LAY A  A+QGDPIDWVSTHR HH+F D+ERDPHSP  GFWFS + W
Sbjct: 90  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLW 149

Query: 138 FFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDLERQAFYRFMHKTYFLHPL 197
            +DS  L  K                       G   NV DL+RQ FYRF+ KT   H L
Sbjct: 150 IYDSAYLVSKC----------------------GRRANVEDLKRQWFYRFLQKTVLFHIL 209

Query: 198 ALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGKQPWNTGDLSRNNWLVALF 257
            L   L+ +GG+ F+ WGM V   L +H+T  +NS+CHIWG + W T D SRN W +++F
Sbjct: 210 GLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVF 269

Query: 258 TYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLATNVKLPSEKYKQKLA 315
           ++GE WHNNHHAFE SAR G +WWQID+ WY+++F ++IGLAT+VK+P+E  ++++A
Sbjct: 270 SFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQRRRMA 304

BLAST of Tan0021488 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 312.8 bits (800), Expect = 3.4e-85
Identity = 146/291 (50.17%), Postives = 185/291 (63.57%), Query Frame = 0

Query: 17  RLFRRKKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLVLANLTGMAISVSYHRNL 76
           R F  + WT  D      F+  HLL + APF F+W    +   L  + G+ I++SYHRNL
Sbjct: 84  RSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICITLSYHRNL 143

Query: 77  AHRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIYGFWFSQMT 136
           +HRSF L KWLEY  AY    A QGDPI+WVS HR HH+  +T+RDPHSP  GFWFS M 
Sbjct: 144 SHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMA 203

Query: 137 WFFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDLERQAFYRFMHKTYFLHP 196
           W FD+ ++                          G  +NV DL RQ FYRF+ +T  LH 
Sbjct: 204 WIFDTSSILENC----------------------GGEENVDDLVRQPFYRFLQRTVLLHM 263

Query: 197 LALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGKQPWNTGDLSRNNWLVAL 256
           +A S L Y  GG+P ++WG+ +   + LH+T  VNSVCHIWG + WNT D S+NNW VA+
Sbjct: 264 MAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWWVAI 323

Query: 257 FTYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLATNVKLPSE 308
            T GEGWHNNHHAFE+SARHG +WWQ+D+ W +I+FL+ IGLATNVKLP+E
Sbjct: 324 LTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTE 352

BLAST of Tan0021488 vs. TAIR 10
Match: AT1G06100.1 (Fatty acid desaturase family protein )

HSP 1 Score: 308.1 bits (788), Expect = 8.3e-84
Identity = 153/306 (50.00%), Postives = 193/306 (63.07%), Query Frame = 0

Query: 9   KDMVKSPPRLFRRKKWTKLDKDVAFSFLLFHLLCIFAPFCFTWGRLLLSLVLANLTGMAI 68
           K  V+   R +  +KWT+ D   A +    HLLC+ APF + W      ++LA LT + I
Sbjct: 13  KKSVRKEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRFGIILAILTNLCI 72

Query: 69  SVSYHRNLAHRSFKLHKWLEYSLAYLAAHALQGDPIDWVSTHRCHHRFVDTERDPHSPIY 128
           + SYHRNL HRSFKL KWLEY  AY A  ALQGDP+DWVS HR HH+F D++RDPHSPI 
Sbjct: 73  TFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQFTDSDRDPHSPIE 132

Query: 129 GFWFSQMTWFFDSYNLTRKVCPNYFNNFQKIDRTSFVFFLKHGYPDNVTDLERQAFYRFM 188
           GFWFS + W FD+ +  R+ C                     G  +NV DL++Q FYRF+
Sbjct: 133 GFWFSHVLWIFDT-DYIREKC---------------------GRRNNVMDLKQQWFYRFL 192

Query: 189 HKTYFLHPLALSALLYAIGGVPFIIWGMCVRFVLSLHITSTVNSVCHIWGKQPWNTGDLS 248
            KT  LH LA   L+Y  GG+P++ W +    V+  H T  VNS CHI G Q W T D S
Sbjct: 193 KKTLVLHILAFWTLIYLWGGLPYLTWTVGFGGVIGYHGTWLVNSACHICGSQAWQTNDTS 252

Query: 249 RNNWLVALFTYGEGWHNNHHAFEYSARHGHKWWQIDVGWYVIKFLQVIGLATNVKLPSEK 308
           RN W +AL T GE WHNNHHAFE SARHG +W+Q+D+ WY+I F Q +GLATNVKLP++ 
Sbjct: 253 RNVWWLALLTMGESWHNNHHAFETSARHGLEWYQLDITWYLIWFFQALGLATNVKLPTDA 296

Query: 309 YKQKLA 315
            K+K+A
Sbjct: 313 QKRKMA 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949X01.8e-9656.12Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
O657972.0e-8749.33Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=... [more]
P0DOW21.9e-8550.34Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1[more]
Q9SID23.3e-8549.16Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=... [more]
P0DOW35.6e-8554.15Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038899717.14.4e-16482.37palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B... [more]
XP_008451258.14.9e-16382.37PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
XP_011659770.11.4e-16282.37palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumi... [more]
XP_011659261.22.5e-15980.85palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumi... [more]
XP_022959667.16.2e-15882.59palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
Match NameE-valueIdentityDescription
A0A1S3BS492.4e-16382.37palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A0A0KAB11.9e-16081.16FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G3763... [more]
A0A6J1H5663.0e-15882.59palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1KRZ23.9e-15881.65palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A1S3BQG56.7e-15878.79palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
Match NameE-valueIdentityDescription
AT3G15850.11.3e-9756.12fatty acid desaturase 5 [more]
AT1G06080.11.5e-8849.33delta 9 desaturase 1 [more]
AT2G31360.12.3e-8649.1616:0delta9 desaturase 2 [more]
AT3G15870.13.4e-8550.17Fatty acid desaturase family protein [more]
AT1G06100.18.3e-8450.00Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015876Acyl-CoA desaturasePRINTSPR00075FACDDSATRASEcoord: 73..93
score: 36.84
coord: 110..139
score: 47.19
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 12..314
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 64..303
e-value: 4.3179E-69
score: 211.646
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 51..282
e-value: 4.7E-14
score: 52.8
NoneNo IPR availablePANTHERPTHR11351:SF87FATTY ACID DESATURASE, TYPE 1, CORE-RELATEDcoord: 12..314

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0021488.1Tan0021488.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0009979 16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water